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Find video protocols related to scientific articles indexed in Pubmed.
Gilvimarinus polysaccharolyticus sp. nov., an agar-digesting bacterium isolated from seaweed, and emended description of the genus Gilvimarinus.
Int. J. Syst. Evol. Microbiol.
PUBLISHED: 11-14-2014
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A taxonomic study was carried out on strain YN3T, which was isolated from a seaweed sample on the coast of Weihai, China. The bacterium was Gram-stain-negative, rod-shaped, and could grow at pH 5.0-10.0 and 4-32 °C in presence of 0-9.0 % (w/v) NaCl. Strain YN3T was positive for hydrolysis of polysaccharides, such as agar, starch and xylan. The predominant respiratory quinone was ubiquinone-8. The major fatty acids were C16:1?7c and/or iso-C15:0 2-OH, C16:0 and C18:1?7c. The main polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine, and two unidentified glycolipids. The genomic DNA G + C content was 49.4 mol %. Phylogenetic analysis based on the 16S rRNA gene sequences indicated that strain YN3T should be assigned to the genus Gilvimarinus. 'Gilvimarinus agarilyticus' KCTC 23325T and Gilvimarinus chinensis QM42T had the closest phylogenetic relationship with strain YN3T, and showed 97.9 % and 95.8 % sequence similarities, respectively. On the basis of phenotypic, chemotaxonomic and genotypic data and DNA-DNA hybridization studies, we propose that strain YN3T represents a novel species of the genus Gilvimarinus, for which the name Gilvimarinus polysaccharolyticus sp. nov. is proposed. The type strain is YN3T (= KCTC 32438T = JCM 19198T). An emended description of the genus Gilvimarinus is also presented.
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Selective Inhibition of Mutant Isocitrate Dehydrogenase 1 (IDH1) via Disruption of a Metal Binding Network by an Allosteric Small Molecule.
J. Biol. Chem.
PUBLISHED: 11-14-2014
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Cancer-associated point mutations in isocitrate dehydrogenase 1 and 2 (IDH1, IDH2) confer a neomorphic enzymatic activity: the reduction of alpha-ketoglutarate (?KG) to D-2-hydroxyglutaric acid (2HG), which is proposed to act as an oncogenic metabolite by inducing hypermethylation of histones and DNA. While selective inhibitors of mutant IDH1 and IDH2 have been identified and are currently under investigation as potential cancer therapeutics, the mechanistic basis for their selectivity is not yet well-understood. A high-throughput screen for selective inhibitors of IDH1 bearing the oncogenic mutation R132H identified Compound 1, a bis-imidazole phenol that inhibits 2HG production in cells. We investigated the mode of inhibition of Compound 1 and a previously published IDH1 mutant inhibitor with a different chemical scaffold. Steady-state kinetics and biophysical studies show that both of these compounds selectively inhibit mutant IDH1 by binding to an allosteric site, and that inhibition is competitive with respect to Mg(2+). A crystal structure of Compound 1 complexed with R132H IDH1 indicates that the inhibitor binds at the dimer interface and makes a direct contact with a residue involved in binding of the catalytically essential divalent cation. These results show that targeting a divalent cation binding residue can enable selective inhibition of mutant IDH1, and suggest that differences in magnesium binding between wild-type and mutant enzymes may contribute to the inhibitors' selectivity for the mutant enzyme.
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Muriicola marianensis sp. nov., a novel bacterium isolated from Mariana Trench.
Int. J. Syst. Evol. Microbiol.
PUBLISHED: 11-08-2014
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A Gram-negative, aerobic, orange-pigmented, rod-shaped and non-motile bacterium, designated A6B8T, was isolated from the seawater of the Mariana Trench. The isolate grew at 4-50 °C (optimum 30-35 °C), pH 6.5-8.0 (optimum pH 7.5) and with 0.5-4.0% (w/v) NaCl (optimum 1.0-2.0%). The phylogenetic analysis based on 16S rRNA gene sequences revealed that strain A6B8T was most closely related to the genus Muriicola by sharing the highest sequence similarity of 97.7% with M. jejuensis EM44T. Chemotaxonomic analysis showed MK-6 was the predominant isoprenoid and iso-C15 : 0, iso-C15 : 1 G and iso-C17 : 0 3-OH were the major cellular fatty acids. The polar lipids profile of strain A6B8T included one phosphatidylethanolamine, three unidentified aminolipids and four unidentified lipids. The genomic DNA G+C content was 47.1 mol%. The DNA-DNA relatedness value (23.3 %) clearly demonstrated that strains A6B8T and M. jejuensis EM44T were representatives of two different species. Based on the phenotypic, phylogenetic and chemotaxonomic characterizations, strain A6B8T (= CGMCC 1.12606T = KCTC 32436T) is considered to represent a novel species of the genus Muriicola, for which the name Muriicola marianensis sp. nov. is proposed.
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[An in vitro comparison of the influence of different surface treatment on shear bond strength between zirconia and enamel].
Shanghai Kou Qiang Yi Xue
PUBLISHED: 10-24-2014
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To compare the shear bond strength of zirconia to enamel using different surface treatments, in order to provide evidence for clinical practice.
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[Study on HPLC fingerprint of Hedyotis chrysotricha].
Zhong Yao Cai
PUBLISHED: 10-23-2014
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To establish HPLC fingerprint of Hedyotis chrysotricha.
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[Study on ecological suitability regionalization of Eucommia ulmoides in Guizhou].
Zhong Yao Cai
PUBLISHED: 10-23-2014
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To study the ecological suitability regionalization of Eucommia ulmoides, for selecting artificial planting base and high-quality industrial raw material purchase area of the herb in Guizhou.
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Phototherapy: Designing Multi-Branched Gold Nanoechinus for NIR Light Activated Dual Modal Photodynamic and Photothermal Therapy in the Second Biological Window (Adv. Mater. 39/2014).
Adv. Mater. Weinheim
PUBLISHED: 10-17-2014
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Gold nanoechinus can act as dual modal nanomaterial-mediated photodynamic therapy and photothermal therapy reagents for complete destruction of solid tumors upon NIR light irradiation, as discussed by K. C. Hwang and co-workers on page 6689.
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Rh(III)-catalyzed dual directing group assisted sterically hindered C-H bond activation: a unique route to meta and ortho substituted benzofurans.
Org. Biomol. Chem.
PUBLISHED: 10-15-2014
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A new strategy for the synthesis of highly substituted benzofurans from meta-substituted hydroxybenzenes and alkynes via a rhodium(iii)-catalyzed activation of a sterically hindered C-H bond is demonstrated. A possible mechanism involving dual directing group assisted ortho C-H bond activation is proposed.
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?-Carotene As a Lipophilic Scavenger of Nitric Oxide.
J Phys Chem B
PUBLISHED: 09-26-2014
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The efficient bleaching following continuous bubbling of gaseous nitric oxide (NO(•)) to ?-carotene (?-Car) dissolved in n-hexane under anaerobic conditions results from an initial addition of two NO(•) followed by fragmentation coupled with further NO(•) addition as shown by mass spectrometry (MS). Density functional theory (DFT) calculations demonstrated that hydrogen atom transfer (HAT) and electron transfer (ET) from ?-Car to NO(•) are strongly energetically unfavorable in contrast to radical adduct formation (RAF) followed by degradation. The results indicated the lowest energy for addition of the first NO(•) at C7 with an activation free energy of ?G(?) = 74.40 kJ mol(-1) and a rate constant of 0.56 s(-1), followed by trans-addition of a second NO(•) at C8 with ?G(?) = 55.51 kJ mol(-1). MS confirmed the formation of a dinitrosyl-?-Car (596.6 m/z), and of a ?-Car fragment (400.4 m/z) formed by C7/C8 bond cleavage and suggested to be of importance for progression of bleaching. Up to eight reaction products with increasing mass of 28 m/z are assigned to continuous addition of NO(•) to the initially formed fragment forming nitroxides. Continuous wave photolysis of sodium nitroprusside (SNP) as a NO(•) source dissolved together with ?-Car in 4:1 (v/v) methanol:tetrahydrofuran gradually bleached ?-Car. Nanosecond laser flash photolysis at 355 nm followed by transient absorption spectroscopy showed a ?-Car derived intermediate with an absorption maximum around 420 nm in agreement with a prediction (425 nm) from time-dependent DFT (TDDFT) for the trans-C7,8 dinitrosyl adduct of ?-Car. The NO(•) adduct of ?-Car decays with a rate constant of ?10(7) s(-1) at 25 °C.
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Total glucosides of peony attenuates 2,4,6-trinitrobenzene sulfonic acid/ethanol-induced colitis in rats through adjustment of TH1/TH2 cytokines polarization.
Cell Biochem. Biophys.
PUBLISHED: 09-24-2014
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The present study is to investigate effects of total glucosides of peony (TGP) on 2,4,6-trinitrobenzene sulfonic acid (TNBS)/ethanol-induced colitis in rats and to explore potential clinical use of TGP for treatment of inflammatory bowel disease. Sixty Sprague-Dawley rats were randomly grouped into normal controls, model controls, sulfasalazine (SASP) controls (100 mg/kg/day), and low, medium, and high-dose TGP groups (25, 50, and 100 mg/kg/day, respectively). 24 h following colonic instillation of TNBS, TGP, and SASP were given by gastric gavage three times a day for 7 days. Disease activity index (DAI), colon macroscopic damage index (CMDI), histopathological score (HPS), and myeloperoxidase (MPO) activity were evaluated. Levels of serum TNF-?, IL-1?, and IL-10 were measured by ELISA, and expression of TNF-?, IL-1?, and IL-10 mRNA and protein in colonic tissues was detected by RT-PCR and western blot, respectively. Compared with rats in the model controls, TGP (50 or 100 mg/kg/day)-treated rats with TNBS/ethanol-induced colitis showed significant improvements of DAI, CMDI, HPS, and MPO activity. Moreover, administration of TGP (50 or 100 mg/kg/day) decreased the up-regulated levels of serum TNF-? and IL-1?, and expression of TNF-? and IL-1? mRNA and protein in colonic tissues, and increased the serum IL-10 and colonic IL-10 mRNA and protein level. And there was no significant difference compared with administration of SASP (P > 0.05). TGP attenuates TNBS/ethanol-induced colitis in rats and its efficacy is similar to SASP, the potential mechanism might be related to the adjustment of Th1/Th2 cytokines polarization by decreasing pro-inflammatory cytokine TNF-? and IL-1?, and increasing anti-inflammatory cytokine IL-10.
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Draft genomic DNA sequence of strain Halomonas sp. FS-N4 exhibiting high catalase activity.
Mar Genomics
PUBLISHED: 08-29-2014
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Halomonas sp. FS-N4 is a bacterium, which can grow in the medium Marine Broth 2216 with 5M initial hydrogen peroxide concentration, shows a strong oxidation resistance, and the crude enzyme activity can reach as high as 13.33katal/mg. We reported the draft genome sequence of H. sp. FS-N4, showing that it contains 3434 protein-coding genes, including the genes putatively involved in the response to the oxidative stress, among which a phytochrome-like gene might be a key point to survive in the environment with high concentration of hydrogen peroxide and exhibit high catalase activity.
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A pH-responsive prodrug for real-time drug release monitoring and targeted cancer therapy.
Chem. Commun. (Camb.)
PUBLISHED: 08-23-2014
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A novel cancer targeting and pH-responsive prodrug was successfully designed and synthesized. This M-prodrug was demonstrated to have real-time drug release monitoring capability based on the concept of contact-mediated quenching between doxorubicin and a coumarin derivative.
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Novel Inhibitory Effects of Glycyrrhizic Acid on the Accumulation of Advanced Glycation End Product and Its Receptor Expression.
Nat Prod Bioprospect
PUBLISHED: 08-17-2014
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Beneficial effects of glycyrrhizic acid (GA), a bioactive extract of licorice root, in the prevention of metabolic syndrome have been consistently reported while advanced glycation end products (AGE) and receptor for advanced glycation end product (RAGE) are the leading factors in the development of diabetes mellitus. The aim of this study was to investigate the effects of GA on the AGE-RAGE axis using high-fat/high-sucrose (HF/HS) diet-induced metabolic syndrome rat models. Twenty four male Sprague-Dawley rats were randomly assigned into three groups for 4 weeks: (1) Group A, normal diet with standard rat chow; (2) Group B, HF/HS diet; (3) Group C, HF/HS diet and oral administration of 100 mg/kg GA per day. The results showed that HF/HS diet elevated the fasting blood glucose level and insulin resistance index which was prevented by GA supplementation. GA treatment significantly lowered the circulating AGE independent of its glucose-lowering effect. HF/HS diet also triggered RAGE upregulation in the abdominal muscles while GA administration downregulated RAGE expression in the abdominal muscles, aorta and subcutaneous adipose tissues. In conclusion, HF/HS diet could cause glucose intolerance, insulin resistance and upregulation of RAGE expression while GA ameliorated the metabolic dysregulation besides exhibiting inhibitory effects on the AGE-RAGE axis.
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Attenuation of neuropathic pain by saikosaponin a in a rat model of chronic constriction injury.
Neurochem. Res.
PUBLISHED: 08-09-2014
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Despite immense advances in the treatment strategies, the effective treatment of patients suffering from neuropathic pain remains challenging. Saikosaponin a possesses anti-inflammatory activity. However, the role of saikosaponin a in neuropathic pain is still unclear. Therefore, the objective of this study was to investigate the effects of saikosaponin a on neuropathic pain. Neuropathic pain was induced by chronic constriction injury (CCI) of the sciatic nerve in rats. After CCI, rats were administered saikosaponin a (6.25, 12.50 and 25.00 mg/kg intraperitoneal, once daily) for 14 days. Mechanical withdrawal threshold and thermal withdrawal latency were assessed before surgery and on days 1, 3, 7, and 14 after CCI. Our results showed that CCI significantly decreased mechanical withdrawal threshold and thermal withdrawal latency on days 1, 3, 7 and 14, as compared with sham groups, however, saikosaponin a reversed this effects. In addition, saikosaponin a inhibited CCI-induced the levels of TNF-?, IL-1?, IL-2 in spinal cord. Western blot analysis demonstrated that saikosaponin a reduced the elevated expression of p-p38 mitogen-activated protein kinase (MAPK) and NF-?B in the spinal cord induced by CCI. These results suggest that saikosaponin a could effectively attenuate neuropathic pain in CCI rats by inhibiting the activation of p38 MAPK and NF-?B signaling pathways in spinal cord.
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Cobalt-catalyzed hydroarylative cyclization of 1,6-enynes with aromatic ketones and esters via C-H activation.
Org. Lett.
PUBLISHED: 08-06-2014
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A highly chemo- and stereoselective cobalt-catalyzed hydroarylative cyclization of 1,6-enynes with aromatic ketones and esters to synthesize functionalized pyrrolidines and dihydrofurans is described. A mechanism involving cobaltacycle triggered C-H activation of aromatic ketones and esters was proposed.
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[The current status of physical activity in urban school-aged children and its association with obesity].
Zhonghua Liu Xing Bing Xue Za Zhi
PUBLISHED: 07-11-2014
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To understand the current status of physical activity among urban school-aged children and its association with obesity.
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[Trends on the prevalence rates of obesity and cardiometabolic among children and adolescents in Beijing, during 2004-2013].
Zhonghua Liu Xing Bing Xue Za Zhi
PUBLISHED: 07-11-2014
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To analyze the trends on the prevalence rates of obesity and cardiometabolic among children and adolescents in Beijing, during 2004-2013.
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PIM inhibitors target CD25-positive AML cells through concomitant suppression of STAT5 activation and degradation of MYC oncogene.
Blood
PUBLISHED: 07-08-2014
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Postchemotherapy relapse presents a major unmet medical need in acute myeloid leukemia (AML), where treatment options are limited. CD25 is a leukemic stem cell marker and a conspicuous prognostic marker for overall/relapse-free survival in AML. Rare occurrence of genetic alterations among PIM family members imposes a substantial hurdle in formulating a compelling patient stratification strategy for the clinical development of selective PIM inhibitors in cancer. Here we show that CD25, a bona fide STAT5 regulated gene, is a mechanistically relevant predictive biomarker for sensitivity to PIM kinase inhibitors. Alone or in combination with tyrosine kinase inhibitors, PIM inhibitors can suppress STAT5 activation and significantly shorten the half-life of MYC to achieve substantial growth inhibition of high CD25-expressing AML cells. Our results highlight the importance of STAT5 and MYC in rendering cancer cells sensitive to PIM inhibitors. Because the presence of a CD25-positive subpopulation in leukemic blasts correlates with poor overall or relapse-free survival, our data suggest that a combination of PIM inhibitors with chemotherapy and tyrosine kinase inhibitors could improve long-term therapeutic outcomes in CD25-positive AML.
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Impact of microRNA-134 on neural cell survival against ischemic injury in primary cultured neuronal cells and mouse brain with ischemic stroke by targeting HSPA12B.
Brain Res.
PUBLISHED: 07-05-2014
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As a newly discovered member of the HSP70 family, heat shock protein A12B (HSPA12B) is involved in brain ischemic injury. According to our previous study, microRNA-134 (miR-134) could target HSPA12B by binding to its 3'-untranslated region (UTR). However, the regulation of miR-134 on HSPA12B and their role in protecting neuronal cells from ischemic injury are unclear. In this study, the miR-134 expression level was manipulated, and the HSPA12B protein levels were also determined in oxygen-glucose deprivation (OGD)-treated primary cultured neuronal cells in vitro and mouse brain after middle cerebral artery occlusion (MCAO)-induced ischemic stroke in vivo. The results showed that miR-134 expression levels increased in primary cultured neuronal cells and mouse brain from 12h to 7 day reoxygenation/reperfusion after 1h OGD or 1h MCAO treatment. miR-134 overexpression promoted neuronal cell death and apoptosis by decreasing HSPA12B protein levels. Conversely, downregulating miR-134 reduced neuronal cell death and apoptosis by enhancing HSPA12B protein levels. Also, HSPA12B siRNA could block miR-134 inhibitor-mediated neuroprotection against OGD-induced neuronal cell injury in vitro. Taken together, miR-134 might influence neuronal cell survival against ischemic injury in primary cultured neuronal cells and mouse brain with ischemic stroke by negatively modulating HSPA12B protein expression in a posttranscriptional manner.
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90d Exposure to Nonylphenol has Adverse Effects on the Spermatogenesis and Sperm Maturation of Adult Male Rats.
Biomed. Environ. Sci.
PUBLISHED: 07-03-2014
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This study was conducted to elucidate the reproductive effect of NP on testis, epididymis and epididymal sperm in vivo. Adult male Sprague-Dawley rats were gavaged with NP at 0, 40, 100, or 250 mg/kg body weight (bw) on alternate days for 90 d. The results showed that oral administration of NP may damage the structure and function of testis, induce apoptosis and oxidative stress in epididymis or even have cytotoxic effects on epididymal sperm.
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[Dietary habits of school-age children and its associations with blood pressure level in Beijing, China].
Zhonghua Yu Fang Yi Xue Za Zhi
PUBLISHED: 07-03-2014
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To study the dietary habits of school-age children in urban and rural districts and their association with blood pressure levels in Beijing, China.
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Spatial distribution and source apportionment of atmospheric dust fall at Beijing during spring of 2008-2009.
Environ Sci Pollut Res Int
PUBLISHED: 05-22-2014
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Beijing is a megacity, where atmospheric dust fall amount is great, and its resultant air pollution is serious. So, analyzing the chemical elements in atmospheric dust fall and revealing its various sources can provide a scientific basis for taking effective measures to improve atmospheric environmental quality. In this paper, we investigated the spatial and temporal distribution of dust fall in Beijing, based on the dust samples collected in the spring of 2008 and 2009 at 18 observation sites laid out in Beijing and then analyzed the sources of atmospheric dust fall based on the test of samples, adopting enrichment factor and factor analysis methods. Our results found that the dust fall quantity in the observation periods was respectively 33.6230 t km(-2) and 28.7130 t km(-2); the dust fall quantity varied significantly in different months in the spring, but the variation trend was similar at the sites. There were two centers of large quantity in Beijing; one was in the southwest of downtown, and the other was in the northeast of downtown. The spatial distribution of dust fall generally showed a structural feature of three loops; the northwestern mountainous area was a small quantity belt; the plain area around the downtown was a large quantity belt, and the central downtown was a center of small quantity. Soil dust, construction dust, coal dust, and vehicle exhaust were the four major sources of dust fall in spring of Beijing, respectively, accounting for 38.50, 22.25, 14.06, and 20.82 % of the total dust fall quantity.
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Tetracycline- and furazolidone-containing quadruple regimen as rescue treatment for Helicobacter pylori infection: a single center retrospective study.
Helicobacter
PUBLISHED: 05-21-2014
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Eradication rate of Helicobacter pylori decreases worldwide, while antibiotics resistance rates of H. pylori increase rapidly in recent years. In most cases, H. pylori would be resistant to clarithromycin, metronidazole, and quinolone if these antibiotics had been used as component of eradication regimen. H. pylori strains resistant to both tetracycline and furazolidone are rare. The aim of our study was to evaluate efficacy and side effects of tetracycline- and furazolidone-containing quadruple regimen as rescue treatment.
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Combination of PIM and JAK2 inhibitors synergistically suppresses MPN cell proliferation and overcomes drug resistance.
Oncotarget
PUBLISHED: 05-17-2014
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Inhibitors of JAK2 kinase are emerging as an important treatment modality for myeloproliferative neoplasms (MPN). However, similar to other kinase inhibitors, resistance to JAK2 inhibitors may eventually emerge through a variety of mechanisms. Effective drug combination is one way to enhance therapeutic efficacy and combat resistance against JAK2 inhibitors. To identify potential combination partners for JAK2 compounds in MPN cell lines, we performed pooled shRNA screen targeting 5,000 genes in the presence or absence of JAK2 blockade. One of the top hits identified was MYC, an oncogenic transcription factor that is difficult to inhibit directly, but could be targeted by modulation of upstream regulatory elements such as kinases. We demonstrate herein that PIM kinase inhibitors efficiently suppress MYC protein levels in MPN cell lines. Importantly, overexpression of MYC restores the viability of PIM inhibitor-treated cells, revealing causal relationship between MYC down-regulation and cell growth inhibition by PIM compounds. Combination of various PIM inhibitors with a JAK2 inhibitor results in significant synergistic growth inhibition of multiple MPN cancer cell lines and induction of apoptosis. Mechanistic studies revealed strong downregulation of phosphorylated forms of S6 and 4EBP1 by JAK2/PIM inhibitor combination treatment. Finally, such combination was effective in eradicating in vitro JAK2 inhibitor-resistant MPN clones, where MYC is consistently up-regulated. These findings demonstrate that simultaneous suppression of JAK2 and PIM kinase activity by small molecule inhibitors is more effective than either agent alone in suppressing MPN cell growth. Our data suggest that JAK2 and PIM combination might warrant further investigation for the treatment of JAK2-driven hematologic malignancies.
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Effects of lycopene on number and function of human peripheral blood endothelial progenitor cells cultivated with high glucose.
Nutr Res Pract
PUBLISHED: 05-15-2014
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The objectives of this study were to investigate the effects of lycopene on the migration, adhesion, tube formation capacity, and p38 mitogen-activated protein kinase (p38 MAPK) activity of endothelial progenitor cells (EPCs) cultivated with high glucose (HG) and as well as explore the mechanism behind the protective effects of lycopene on peripheral blood EPCs.
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One-pot synthesis of highly substituted polyheteroaromatic compounds by rhodium(III)-catalyzed multiple C-H activation and annulation.
Angew. Chem. Int. Ed. Engl.
PUBLISHED: 05-11-2014
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A new method for the synthesis of highly substituted naphthyridine-based polyheteroaromatic compounds in high yields proceeds through rhodium(III)-catalyzed multiple C-H bond cleavage and C-C and C-N bond formation in a one-pot process. Such highly substituted polyheteroaromatic compounds have attracted much attention because of their unique ?-conjugation, which make them suitable materials for organic semiconductors and luminescent materials. Furthermore, a possible mechanism, which involves multiple chelation-assisted ortho C-H activation, alkyne insertion, and reductive elimination, is proposed for this transformation.
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Alkene-assisted nickel-catalyzed regioselective 1,4-addition of organoboronic acid to dienones: a direct route to all-carbon quaternary centers.
Org. Lett.
PUBLISHED: 05-09-2014
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A nickel-catalyzed highly regioselective 1,4-addition reaction of boronic acids to dienones to form products with an all-carbon quaternary center is described. The 3-alkenyl group of dienones is the key for the reaction to proceed smoothly. A mechanism involving the coordination of the dienyl group to the nickel center is proposed.
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Draft genome sequence of Microbulbifer elongatus strain HZ11, a brown seaweed-degrading bacterium with potential ability to produce bioethanol from alginate.
Mar Genomics
PUBLISHED: 05-07-2014
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Microbulbifer elongatus strain HZ11, was a new strain of M. elongates DSM 6810(T), which has the ability to degrade brown seaweeds such as Laminaria japonica into single cell detritus particles. Here we report a high quality draft genome of M. elongatus strain HZ11, which comprises 4,223,108bp in 9 contigs with the G+C content of 56.70%. A total of 3293 protein-coding sequences were predicted, including nine alginate lyases (EC 4.2.2.3), five agarases (EC 3.2.1.81), 2-dehydro-3-deoxygluconate kinase (EC 2.7.1.45) and all enzymes involved in the Entner-Doudoroff pathway. Our results suggest that strain HZ11 has the potential ability to produce bioethanol from alginate with moderate genetic modification, which may significantly increase the yield of bioethanol from brown seaweed and the utilization rate of brown seaweeds.
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A Sub-Element in PRE enhances nuclear export of intronless mRNAs by recruiting the TREX complex via ZC3H18.
Nucleic Acids Res.
PUBLISHED: 04-29-2014
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Viral RNA elements that facilitate mRNA export are useful tools for identifying cellular RNA export factors. Here we show that hepatitis B virus post-transcriptional element (PRE) is one such element, and using PRE several new cellular mRNA export factors were identified. We found that PRE drastically enhances the cytoplasmic accumulation of cDNA transcripts independent of any viral protein. Systematic deletion analysis revealed the existence of a 116 nt functional Sub-Element of PRE (SEP1). The RNP that forms on the SEP1 RNA was affinity purified, in which TREX components as well as several other proteins were identified. TREX components and the SEP1-associating protein ZC3H18 are required for SEP1-mediated mRNA export. Significantly, ZC3H18 directly binds to the SEP1 RNA, interacts with TREX and is required for stable association of TREX with the SEP1-containing mRNA. Requirements for SEP1-mediated mRNA export are similar to those for splicing-dependent mRNA export. Consistent with these similarities, several SEP1-interacting proteins, including ZC3H18, ARS2, Acinus and Brr2, are required for efficient nuclear export of polyA RNAs. Together, our data indicate that SEP1 enhances mRNA export by recruiting TREX via ZC3H18. The new mRNA export factors that we identified might be involved in cap- and splicing-dependent TREX recruitment to cellular mRNAs.
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[Comparison of chromosome karyotype between myelodysplastic syndrome and acute leukemia patients confirmed at the same period].
Zhongguo Shi Yan Xue Ye Xue Za Zhi
PUBLISHED: 04-26-2014
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This study was purposed to compare and analyze the relationship between the abnormality of chromosome karyotypes and diagnosis, prognosis of MDS and AML patients, as well as to explore the characteristics of chromosome prognostic stratification in MDS and AML patients of different ages. The cytogenetic karyotype analysis was performed in 134 cases of MDS and 123 cases of AML by using bone marrow short-term culture and R-banding technique. The results indicated that the detected rates of chromosome abnormal karyotypes in MDS and AML patients were 41% and 61% respectively. The abnormal karyotype analysis of MDS and AML group showed that the abnormal karyotype in MDS group displayed number abnormality as the dominate (mainly the +8), while the abnormal karyotype in AML group displayed structure abnormality as the dominant [mainly, t(15;17) and t(8;21)]. The detected abnormal karyotype are mainly for the +8 which has ambiguous correlation with FAB subtype; the detection rates of complex karyotype abnormalities, favourable prognosis karyotype as well as poor prognosis karyotype in the MDS group obviously higher than that of AML group. Among patients with MDS transformed into AML, 12 cases had chromosome abnormal karyotype. There were 3 cases of chromosome abnormal karyotype in AML group which were transformed by MDS. The analysis of age stratification between two groups showed that the detected rate of abnormal karyotype was enhanced with the increase of age in MDS group, and detected rate in ? 60 years old group was obviously higher than that in patients with ? 30 age group.The detected rate of complex karyotype abnormalities in three age groups of MDS did not show statistical difference; the detected rate of abnormal karyotype in AML group decreased with the increase of age, the detected rate in ? 30 years old group was obviously higher than that in ? 60 age group,while the detection rate of complex karyotype abnormalities showed that the detected rate in patients ? 60 years old group was obviously higher than that in patients with ? 30 years old group; Analysis of karyotype prognosis revealed that the detected rate of poor prognosis karyotype increased along with the age growth both in MDS and AML groups, and detected rate in ? 60 years old group was obviously higher than that in ? 30 years old group; while analysis of favourable prognosis karyotype in MDS and AML group showed that the detected rate in ? 30 years old group was obviously higher than that in ? 60 years old group. It is concluded that the patients with MDS and AML have higher chromosomal abnormalities,which have important reference value for the diagnosis, treatment and prognosis, meanwhile, the analysis of chromosome karyotype provides an important basis for prognostic stratification.
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[Efficacy and safety of 14-day amoxicillin and furazolidone-based quadruple rescue regimen for eradication of Helicobacter pylori: a retrospective study].
Zhonghua Yi Xue Za Zhi
PUBLISHED: 04-26-2014
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To evaluate the efficacy, compliance and adverse effects of 14-day amoxicillin and furazolidone-based quadruple regimen as rescue treatment for Helicobacter pylori (H.pylori) infection.
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Platinum sensitivity and CD133 expression as risk and prognostic predictors of central nervous system metastases in patients with epithelial ovarian cancer.
BMC Cancer
PUBLISHED: 04-09-2014
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To characterize prognostic and risk factors of central nervous system (CNS) metastases in patients with epithelial ovarian cancer (EOC).
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[Association between different types of obesity and cardiovascular risk factors in school-aged children].
Zhonghua Liu Xing Bing Xue Za Zhi
PUBLISHED: 04-02-2014
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The aim of this paper was to analyze the association between different types of obesity and cardiovascular risk factors (CRFs)in school-aged children.
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Differential expression of alpha-adrenoceptor subtypes in rat dorsal root ganglion after chronic constriction injury.
J. Huazhong Univ. Sci. Technol. Med. Sci.
PUBLISHED: 03-24-2014
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mRNAs of alpha-adrenoceptor (?-AR) subtypes are found in neurons in dorsal root ganglion (DRG) and change after peripheral nerve injury. In this study, the distribution of ?-AR subtype proteins was studied in L5 DRG of normal rats and rats with chronic constriction injury of sciatic nerve (CCI). Using immunofluorescence technique, it was found that ?1A-, ?1B-, and ?2A-AR proteins were expressed in large, medium, and small size neurons in normal DRG, and significantly increased in all size neurons 14 days after CCI. ?1D- and ?2C-AR was also expressed in all size neurons in normal DRG. However, ?1D-AR was significantly increased and ?2C-AR was decreased in small size neurons 14 days post CCI. ?2B-AR neurons were not detectable in normal and CCI DRG. Co-expression of ?1A- and ?2A-AR in the same neuron was observed in normal DRG and increased post CCI. Collectively, these results indicated that there is distinct distribution of ?-AR subtypes in DRG neurons, and the distribution and levels of expression of ?-AR subtypes change differently after CCI. The up-regulation of ?-AR subtypes in DRG neurons may play an important role in the process of generating and transmitting neuropathic pain.
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[Research progress of bonding strength between porcelain veneer and enamel].
Shanghai Kou Qiang Yi Xue
PUBLISHED: 03-11-2014
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Porcelain veneer had gained more and more attention in dental clinical applications due to its advantages such as good esthetic effects and minor invasiveness. The reliable and consistent adhesive bonding were the key to success. The enamel which featured high mineralization and low moisture would be the ideal bonding part for porcelain veneer. This article was aimed to summarize the research progress regarding to those factors that might had effect on the bonding strength between the porcelain veneer and the enamel including the restoration types of resin adhesives and bonding surface preparations.
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Prevalence of adiposity and associated cardiometabolic risk factors in the samoan genome-wide association study.
Am. J. Hum. Biol.
PUBLISHED: 03-10-2014
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To describe the prevalence of obesity-related noncommunicable diseases (NCDs) and associated risk factors in a sample of Samoan adults studied in 2010 as part of a genome-wide assocation study (GWAS) for obesity related traits.
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An obesity genetic risk score predicts risk of insulin resistance among Chinese children.
Endocrine
PUBLISHED: 02-14-2014
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A great number of body mass index (BMI)/obesity-related loci have been identified by recent genome-wide association studies. The objective of the study is to investigate the associations of 11 obesity-related loci with insulin resistance (IR) in a Chinese children population. Participants included 3,468 Chinese children, aged 6-18 years. The 75 percentile (equal to 2.93) of homeostasis model assessment of IR (HOMA-IR) index was considered as the cut-off of IR. A total of 868 IR cases and 2,600 control children were identified. In age- and sex-adjusted model, only two SNPs in/near GNPDA2 and KCTD15 genes were significantly associated with risk of IR [GNPDA2 rs10938397: allelic odds ratio (OR) = 1.19, 95 % confidence interval (CI) 1.06-1.34, P = 0.003; KCTD15 rs29941: allelic OR = 1.15, 95 % CI = 1.01-1.31, P = 0.034]; genetic risk score was also significantly associated risk of IR (OR = 1.08, 95 % 1.04-1.12, P = 1.18 × 10(-4)). After additional adjustment for BMI, none remained significant. The associations of GNPDA2 rs10938397 and the SNPs in combination with risk of IR remained statistically significant after correction for multiple testing. The present study demonstrated that the associations of GNPDA2 rs10938397 and the SNPs in combination with risk of IR were statistically significant, which were dependent on BMI.
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Designing Multi-Branched Gold Nanoechinus for NIR Light Activated Dual Modal Photodynamic and Photothermal Therapy in the Second Biological Window.
Adv. Mater. Weinheim
PUBLISHED: 02-13-2014
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Gold nanoechinus can sensitize the formation of singlet oxygen in the first and the second near-infra red (NIR) biological windows and exert in vivo dual modal photodynamic and photothermal therapeutic effects (PDT and PTT) to destruct the tumors completely. This is the first literature example of the destruction of tumors in NIR window II induced by dual modal nanomaterial-mediated photodynamic and photothermal therapy (NmPDT & NmPTT).
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Arginyltransferase ATE1 catalyzes midchain arginylation of proteins at side chain carboxylates in vivo.
Chem. Biol.
PUBLISHED: 02-13-2014
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Arginylation is an emerging posttranslational modification mediated by Arg-tRNA-protein-transferase (ATE1). It is believed that ATE1 links Arg solely to the N terminus of proteins, requiring prior proteolysis or action by Met-aminopeptidases to expose the arginylated site. Here, we tested the possibility of Arg linkage to midchain sites within intact protein targets and found that many proteins in vivo are modified on the side chains of Asp and Glu by unconventional chemistry that targets the carboxy rather than the amino groups at the target sites. Such arginylation appears to be functionally regulated, and it can be directly mediated by ATE1, in addition to the more conventional ATE1-mediated linkage of Arg to the N-terminal alpha amino group. This midchain arginylation implies an unconventional mechanism of ATE1 action that likely facilitates its major biological role.
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Increased serum amyloid A and its association with autoantibodies, acute phase reactants and disease activity in patients with rheumatoid arthritis.
Mol Med Rep
PUBLISHED: 01-25-2014
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Determination of disease activity in patients with rheumatoid arthritis (RA) has become an important component for RA management. The aim of the present study was to investigate the association between circulating levels of serum amyloid A (SAA) and disease activity in RA patients. The types of disease and the respective number of patients enrolled in the present study were as follows: RA, 88; osteoarthritis (OA), 54; systemic lupus erythematosus (SLE), 43; and other autoimmune diseases, 30, as well as 50 healthy controls (HC). SAA levels were measured using an ELISA assay and western blot analysis was used to detect serum SAA levels. The correlations between SAA levels and disease activity score for 28 joints (DAS28), erythrocyte sedimentation rate (ESR) and C?reactive protein (CRP), respectively, were evaluated; in addition, the presence and absence of rheumatoid factor (RF) and anti?cyclic citrullinated peptide antibody (anti?CCP) were detected in respect to SAA levels. The results of the present study demonstrated that serum levels of SAA in RA patients were significantly increased compared to those of the OA, SLE, others and HC patients (P<0.05). SAA levels were found to be positively correlated with DAS28, ESR and CRP levels (R2=0.6174, 0.4422 and 0.3919, respectively). In addition, anti?CCP was not correlated with DAS28 (R2=0.0154). Furthermore, increased SAA levels were detected in patients with positive anti?CCP compared with those in anti?CCP negative subjects (P<0.01). In conclusion, the results of the present study provided further evidence for possible roles of SAA in RA, which indicated that it may be a useful biomarker for assessing disease severity and may provide additional information about disease activity.
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Electron transfer from plant phenolates to carotenoid radical cations. Antioxidant interaction entering the Marcus theory inverted region.
J. Agric. Food Chem.
PUBLISHED: 01-21-2014
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?-Carotene, lycopene, and zeaxanthin are maximally regenerated by plant phenolates from their radical cations formed during laser flash photolysis in 9:1 (v/v) chloroform/methanol for a driving force corresponding to the reorganization energy according to the Marcus theory. For ?-carotene, the reorganization energy has values of 0.41 ± 0.04 and 0.40 ± 0.04 eV for the plant phenols in the presence of 1 and 2 equiv of base, respectively, at 23 °C. For a driving force lower than the reorganization energy, regeneration of the carotenoids is less efficient as is seen for m-hydroxybenzoic acid, vanillic acid, and p-coumaric acid. For a driving force above the maximum rate as determined to have kET = 6.3 × 10(9) L·mol(-1)·s(-1) for syringic acid and ?-carotene, the reaction becomes gradually slower and regeneration less efficient as is seen for the more reducing caffeic acid, rutin, and quercetin corresponding to an inverted region for the rate of electron transfer. Lycopene and zeaxanthin show a similar behavior for the same series of plant phenols with slightly lower reorganization energy, in agreement with the lower reduction potential of their radical cations, while, for the ketocarotenoids astaxanthin and canthaxanthin, fast reactions with a solvent of radical cations inhibit regeneration from being detected. Intermediate reducing plant phenols accordingly yield maximal protection of carotenoids against photobleaching in foods and beverages.
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Derivation and validation of a risk score for contrast-induced nephropathy after cardiac catheterization in Chinese patients.
Clin. Exp. Nephrol.
PUBLISHED: 01-16-2014
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To derive and validate a risk score for prediction of contrast-induced nephropathy (CIN) in the Chinese patients undergoing cardiac catheterization.
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Differentiation of Brain Abscesses from Glioblastomas and Metastatic Brain Tumors: Comparisons of Diagnostic Performance of Dynamic Susceptibility Contrast-Enhanced Perfusion MR Imaging before and after Mathematic Contrast Leakage Correction.
PLoS ONE
PUBLISHED: 01-01-2014
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To compare the diagnostic performance of dynamic susceptibility contrast-enhanced perfusion MRI before and after mathematic contrast leakage correction in differentiating pyogenic brain abscesses from glioblastomas and/or metastatic brain tumors.
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Age- and sex-dependent association between FTO rs9939609 and obesity-related traits in Chinese children and adolescents.
PLoS ONE
PUBLISHED: 01-01-2014
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The associations between common variants in the fat mass- and obesity-associated (FTO) gene and obesity-related traits may be age-dependent and may differ by sex. The present study aimed to assess the association of FTO rs9939609 with body mass index (BMI) and the risk of obesity from childhood to adolescence, and to determine the age at which the association becomes evident.
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Ultrafast hydrogen exchange reveals specific structural events during the initial stages of folding of cytochrome c.
J. Am. Chem. Soc.
PUBLISHED: 12-31-2013
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Many proteins undergo a sharp decrease in chain dimensions during early stages of folding, prior to the rate-limiting step in folding. However, it remains unclear whether compact states are the result of specific folding events or a general hydrophobic collapse of the poly-peptide chain driven by the change in solvent conditions. To address this fundamental question, we extended the temporal resolution of NMR-detected H/D exchange labeling experiments into the microsecond regime by adopting a microfluidics approach. By observing the competition between H/D exchange and folding as a function of labeling pH, coupled with direct measurement of exchange rates in the unfolded state, we were able to monitor hydrogen-bond formation for over 50 individual backbone NH groups within the initial 140 microseconds of folding of horse cytochrome c. Clusters of solvent-shielded amide protons were observed in two ?-helical segments in the C-terminal half of the protein while the N-terminal helix remained largely unstructured, suggesting that proximity in the primary structure is a major factor in promoting helix formation and association at early stages of folding while the entropically more costly long-range contacts between the N- and C-terminal helices are established only during later stages. Our findings clearly indicate that the initial chain condensation in cytochrome c is driven by specific interactions among a subset of ?-helical segments rather than a general hydrophobic collapse.
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3,6,9,12-Tetrasubstituted Chrysenes: Synthesis, Photophysical Properties, and Application as Blue Fluorescent OLED.
J. Org. Chem.
PUBLISHED: 12-05-2013
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A short synthesis of unsubstituted chrysene is described to provide a cheap source of this compound. This chrysene was used to prepare 3,6,9,12-tetrabromochrysene, which was subsequently transformed into various 3,6,9,12-tetrasubstituted chrysenes bearing four aryl, alkynyl, or amino groups by means of the Suzuki, Sonogashira, or Buchwald-Hartwig coupling reaction, respectively. These substituents result in large bathochromic shifts in the chrysene absorption and emission spectra. These new chrysene derivatives show blue fluorescent emission (401-471 nm) with high quantum yields (0.44-0.87). DFT calculations on these chrysenes rationalize well the substituent effects on their HOMO and LUMO energy levels. One representative chrysene (6g) was used as a blue fluorescent emitter in an OLED device that showed an outstanding external quantum efficiency (? = 6.31%) with blue emission [CIE (x, y) = (0.13, 0.20)] and a low turn-on voltage (3.0 V).
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One pot synthesis of bioactive benzopyranones through palladium-catalyzed C-H activation and CO insertion into 2-arylphenols.
Chem. Commun. (Camb.)
PUBLISHED: 11-11-2013
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Palladium-catalyzed oxidative carbonylation of 2-arylphenols through C-H bond activation and C-C and C-O bond formation under acid-base free and mild conditions has been developed. The reaction tolerates a variety of substrates and provides biologically important benzopyranone derivatives in up to 87% isolated yield.
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Synthesis of Isoquinolines via Rh(III)-Catalyzed C-H Activation Using Hydrazone as a New Oxidizing Directing Group.
Org. Lett.
PUBLISHED: 10-24-2013
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An efficient and mechanistically interesting method for the synthesis of highly substituted isoquinolines by a Rh(III)-catalyzed hydrazone directed ortho C-H bond activation and annulation without an external oxidant is described. This reaction is accomplished via a C-C and C-N bond formation along with N-N bond cleavage.
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[Risk of obesity-related gene polymorphism on the incidence and durative of childhood obesity].
Zhonghua Liu Xing Bing Xue Za Zhi
PUBLISHED: 10-16-2013
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To examine the impact of single nucleotide polymorphisms(SNPs)in obesity-related genes on the incidence and durative of obesity in childhood and adolescence.
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Congenital cystic adenomatoid malformation with associated mucinous bronchioloalveolar carcinoma in a neonate.
Fetal Pediatr Pathol
PUBLISHED: 10-07-2013
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Congenital cystic adenomatoid malformation (CCAM) of lung is a rare hamartomatous disorder characterized by abnormal branching morphogenesis of the lung. We report an unusual case of a 2-day-old male newborn with a pulmonary cystic lesion and lobectomy revealed a CCAM of the lung that has overlapping features of type 1 and type 2, complicating with multifocal mucinous bronchioloalveolar carcinoma (BAC). The case indicates that malignant transformation can occur in very early stage of the infancy in the patients with CCAM of lung.
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Clinicopathological and Extensive Immunohistochemical Study of a Type II Pleuropulmonary Blastoma.
Fetal Pediatr Pathol
PUBLISHED: 09-30-2013
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Pleuropulmonary blastoma (PPB) is a rare malignant dysontogenetic neoplasm primarily affecting younger children, even in newborns with an unfavorable outcome. PPB is histologically composed of a primitive, variably mixed blastematous and sarcomatous components, and exclusively subclassified as type I (purely cystic), type II (both cystic and solid elements) and type III (completely solid) by increasing histological evidence of malignancy. At present, well-documented cases or cases of truly precise presentation of either pathological or immunohistochemical findings in PPB are rare. The authors report one case of PPB in a 44-month-old child presenting as a solid and cystic mass with special emphasis on its radiological, histopathological and immunohistochemical aspects. The histological diagnosis was PPB, which would belong to the type II category.
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A highly luminescent spiro-anthracenone-based organic light-emitting diode exhibiting thermally activated delayed fluorescence.
Chem. Commun. (Camb.)
PUBLISHED: 09-27-2013
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Efficient thermally activated delayed fluorescence was observed from a spiro-anthracenone derivative (ACRSA). An organic light-emitting diode containing ACRSA exhibited blue-greenish electroluminescence with a maximum external quantum efficiency of 16.5%.
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[Prevalence of nonalcoholic fatty liver disease and metabolic abnormalities in 387 obese children and adolescents in Beijing, China].
Zhonghua Liu Xing Bing Xue Za Zhi
PUBLISHED: 09-11-2013
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To determine the prevalence of nonalcoholic fatty liver disease (NAFLD) and metabolic abnormalities in obese children and adolescents in Beijing.
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Evaluation of cancer dependence and druggability of PRP4 kinase using cellular, biochemical, and structural approaches.
J. Biol. Chem.
PUBLISHED: 09-03-2013
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PRP4 kinase is known for its roles in regulating pre-mRNA splicing and beyond. Therefore, a wider spectrum of PRP4 kinase substrates could be expected. The role of PRP4 kinase in cancer is also yet to be fully elucidated. Attaining specific and potent PRP4 inhibitors would greatly facilitate the study of PRP4 biological function and its validation as a credible cancer target. In this report, we verified the requirement of enzymatic activity of PRP4 in regulating cancer cell growth and identified an array of potential novel substrates through orthogonal proteomics approaches. The ensuing effort in structural biology unveiled for the first time unique features of PRP4 kinase domain and its potential mode of interaction with a low molecular weight inhibitor. These results provide new and important information for further exploration of PRP4 kinase function in cancer.
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Cu(I)-catalyzed intramolecular oxidative C-H amination of 2-aminoacetophenones: a convenient route toward isatins.
Chem. Commun. (Camb.)
PUBLISHED: 08-16-2013
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2-Aminoaryl methyl ketones undergo intramolecular oxidative C-H amination to give the corresponding substituted isatins under an oxygen atmosphere in the presence of [CuI(bpy)]2.
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A convenient synthesis of quinolizinium salts through Rh(III) or Ru(II)-catalyzed C-H bond activation of 2-alkenylpyridines.
Chem. Commun. (Camb.)
PUBLISHED: 08-14-2013
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An efficient synthesis of quinolizinium salts from 2-vinylpyridines and alkynes via Rh(III) or Ru(II)-catalyzed C-H activation and annulation reaction is described. A possible mechanism involving pyridine assisted vinylic ortho-C-H activation, alkyne insertion and reductive elimination is proposed.
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An obesity genetic risk score is associated with metabolic syndrome in Chinese children.
Gene
PUBLISHED: 07-27-2013
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Recent genome-wide association studies have identified several single nucleotide polymorphisms (SNPs) associated with body mass index (BMI)/obesity. In this study, we aim to examine the associations of obesity related loci with risk of metabolic syndrome (MetS) in a children population from China. A total of 431 children with MetS and 3046 controls were identified based on the modified ATPIII definition. 11 SNPs (FTO rs9939609, MC4R rs17782313, GNPDA2 rs10938397, BDNF rs6265, FAIM2 rs7138803, NPC1 rs1805081, SEC16B rs10913469, SH2B1 rs4788102, PCSK1rs6235, KCTD15 rs29941, BAT2 rs2844479) were genotyped by TaqMan 7900. Of 11 SNPs, GNPDA2 rs10938397, BDNF rs6265, and FAIM2 rs7138803 were nominally associated with risk of MetS (GNPDA2 rs10938397: odds ratio (OR)=1.21, 95% confidence interval (CI)=1.04-1.40, P=0.016; BDNF rs6265: OR=1.19, 95% CI=1.03-1.39, P=0.021; FAIM2 rs7138803: OR=1.20, 95% CI=1.02-1.40, P=0.025); genetic risk score (GRS) was significantly associated with risk of MetS (OR=1.09, 95% CI=1.04-1.15, P=5.26×10(-4)). After further adjustment for BMI, none of SNPs were associated with risk of MetS (all P>0.05); the association between GRS and risk of MetS remained nominally (OR=1.02, 95%CI=0.96-1.08, P=0.557). However, after correction for multiple testing, only GRS was statistically associated with risk of MetS in the model without adjustment for BMI. The present study demonstrated that there were nominal associations of GNPDA2 rs10938397, BDNF rs6265, and FAIM2 rs7138803 with risk of MetS. The SNPs in combination have a significant effect on risk of MetS among Chinese children. These associations above were mediated by adiposity.
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Nonuniform chain collapse during early stages of staphylococcal nuclease folding detected by fluorescence resonance energy transfer and ultrarapid mixing methods.
Protein Sci.
PUBLISHED: 07-10-2013
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The development of tertiary structure during folding of staphylococcal nuclease (SNase) was studied by time-resolved fluorescence resonance energy transfer measured using continuous- and stopped-flow techniques. Variants of this two-domain protein containing intradomain and interdomain fluorescence donor/acceptor pairs (Trp and Cys-linked fluorophore or quencher) were prepared to probe the intradomain and interdomain structural evolution accompanying SNase folding. The intra-domain donor/acceptor pairs are within the ?-barrel domain (Trp27/Cys64 and Trp27/Cys97) and the interdomain pair is between the ?-helical domain and the ?-barrel domain (Trp140/Cys64). Time-resolved energy transfer efficiency accompanying folding and unfolding at different urea concentrations was measured over a time range from 30 ?s to ? 10 s. Information on average donor/acceptor distances at different stages of the folding process was obtained by using a quantitative kinetic modeling approach. The average distance for the donor/acceptor pairs in the ?-barrel domain decreases to nearly native values whereas that of the interdomain donor/acceptor pairs remains unchanged in the earliest intermediate (<500 ?s of refolding). This indicates a rapid nonuniform collapse resulting in an ensemble of heterogeneous conformations in which the central region of the ?-barrel domain is well developed while the C-terminal ?-helical domain remains disordered. The distance between Trp140 and Cys64 decreases to native values on the 100-ms time scale, indicating that the ?-helical domain docks onto the preformed ?-barrel at a late stage of the folding. In addition, the unfolded state is found to be more compact under native conditions, suggesting that changes in solvent conditions may induce a nonspecific hydrophobic collapse.
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[The effect of niflumic acid and blocker of calcium channel on the desensitization of gamma aminobutyric acid-activated current].
Zhongguo Ying Yong Sheng Li Xue Za Zhi
PUBLISHED: 07-10-2013
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To explore the modulatory effect of niflumic acid and blocker of calcium channel on the desensitization of gamma aminobutyric acid (GABA)-activated currents in dorsal root ganglion(DRG) neurons from rat.
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Insulin resistance determined by Homeostasis Model Assessment (HOMA) and associations with metabolic syndrome among Chinese children and teenagers.
Diabetol Metab Syndr
PUBLISHED: 07-03-2013
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The aim of this study is to assess the association between the degree of insulin resistance and the different components of the metabolic syndrome among Chinese children and adolescents. Moreover, to determine the cut-off values for homeostasis model assessment of insulin resistance (HOMA-IR) at MS risk.
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Rh(III) -catalyzed C-H activation: a versatile route towards various polycyclic pyridinium salts.
Chemistry
PUBLISHED: 06-17-2013
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An efficient and convenient method for the synthesis of highly substituted polycyclic pyridinium salts from the reaction of various 2-aryl-pyridines and 2-aryl-sp(2) -nitrogen-atom-containing heterocycles with alkynes through rhodium(III)-catalyzed C?H activation and annulation under an O2 atmosphere is described. A possible mechanism that involves the chelation-assisted C?H activation of the 2-aryl-pyridine substrate, insertion of the alkyne, and reductive elimination is proposed. This mechanism was supported by the isolation of a five-membered rhodacycle (I). In addition, kinetic isotope studies were performed to understand the intimate reaction mechanism.
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Common genetic variants associated with lipid profiles in a Chinese pediatric population.
Hum. Genet.
PUBLISHED: 06-15-2013
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Genome-wide association (GWA) studies have identified many candidate genes that are associated with blood lipid and lipoprotein concentrations. In this study, we want to know whether the results from European for lipid-related single-nucleotide polymorphisms (SNPs) are generalizable to Chinese children. We genotyped seven SNPs in Chinese school-age children (n = 3,503) and assessed the associations of these SNPs with lipids profiles and dyslipidemia. After false discovery rate correction, of the seven SNPs, six (rs2144300, p ~ 9.30 × 10(-3); rs1260333, p ~ 6.20 × 10(-11); rs1260326, p ~ 8.73 × 10(-11); rs10105606, p ~ 0.010; rs1748195, p ~ 0.016 and rs964184, p ~ 2.33 × 10(-13)) showed strong association with triglycerides. Three SNPs (rs1260333, p ~ 3.30 × 10(-3); rs1260326, p ~ 4.39 × 10(-3) and rs2954029, p ~ 6.36 × 10(-4)) showed strong association with total cholesterol. Two SNPs (rs10105606, p ~ 6.66 × 10(-4) and rs1748195, p ~ 2.55 × 10(-3)) showed strong association with high density lipoprotein cholesterol. Four SNPs (rs1260333, p ~ 0.017; rs1260326, p ~ 0.013; rs2954029, p ~ 1.09 × 10(-3) and rs964184, p ~ 5.51 × 10(-3)) showed strong association with low density lipoprotein cholesterol. There were significant associations between rs1260333 (OR is 0.82, 95 % CI 0.74-0.92, p ~ 3.96 × 10(-4)), rs1260326 (OR is 0.82, 95 % CI 0.74-0.92, p ~ 5.31 × 10(-4)), and rs964184 (OR is 1.36, 95 % CI 1.20-1.55, p ~ 1.89 × 10(-6)) and dyslipidemia. These SNPs generated strong combined effects on lipid profiles and dyslipidemia. Our study demonstrates that SNPs associated with lipids from European GWA studies also play roles in Chinese children, which broadened the understanding of lipids metabolism.
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Nickel-catalyzed chemo- and stereoselective alkenylative cyclization of 1,6-enynes with alkenyl boronic acids.
Chemistry
PUBLISHED: 06-07-2013
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Discover nickel! A nickel-catalyzed alkenylative cyclization of 1,6-enynes and alkenyl boronic acids affording substituted pyrrolidines and dihydrofurans is described (see scheme; cod = 1,5-cyclooctadiene, Ts = p-toluene sulfonate). The reaction is highly chemo- and stereoselective. A possible reaction mechanism involving a nickelacyclopentene intermediate is proposed.
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[Comparative analysis of main agronomic traits and interrelationships in cultivation provenances of Pesudostellaria heterophylla from Guizhou province].
Zhongguo Zhong Yao Za Zhi
PUBLISHED: 05-31-2013
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To provide guidance for selection and breeding of Pesudostellaria heterophylla, agronomic traits of 3 mainly cultivated form of P. heterophylla were observed and compared in Guizhou shibing.
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Relation between the interval from coronary angiography to selective off-pump coronary artery bypass grafting and postoperative acute kidney injury.
Am. J. Cardiol.
PUBLISHED: 05-25-2013
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The aim of this study was to investigate whether there are effects of intervals between elective off-pump coronary artery bypass grafting (OPCABG) and coronary angiography (CAG) on postoperative acute kidney injury (AKI). The clinical data of patients undergoing OPCABG and CAG from June 2010 to December 2011 in Beijing Anzhen Hospital were retrospectively analyzed. All the patients were divided into AKI and non-AKI groups. Univariate analysis was performed to find possible factors associated with AKI. Multivariate logistic regression analysis was used to identify whether the short interval was one of the independent risk factors of AKI after adjusting for potential confounding variables. Of 1,513 patients, 529 patients (34.9%) developed AKI. The mortality rate in AKI group (4.9%) was >5× higher than that in non-AKI group (0.9%). The incidence of AKI was highest (56.1%) in patients in whom OPCABG was performed ?24 hours after CAG. Multivariate logistic regression analysis showed that the interval of ?24 hours between OPCABG and CAG did increase the risk of AKI (odds ratio 2.15, 95% confidence interval 1.10 to 4.20) after adjusting for the following confounding variables: diabetes mellitus, New York Heart Association heart function class III and IV, lower estimated glomerular filtration rate, numbers of coronary artery bypass grafts ?3, intraoperative or postoperative intra-aortic balloon pump, intraoperative and postoperative red blood cells transfusion of >3 units, postoperative hypotension, dosage of furosemide of >100 mg/day. In conclusion, it was one of the independent risk factors of postoperative AKI that the OPCABG was performed ?24 hours after CAG.
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[The effect of niflumic acid on gamma aminobutyric acid activated current in DRG neurons].
Zhongguo Ying Yong Sheng Li Xue Za Zhi
PUBLISHED: 05-14-2013
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To explore the modulatory effect of niflumic acid (NFA) on gamma aminobutyric acid (GABA)-activated currents of dorsal root ganglion (DRG) neurons in rat.
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[Diagnosis and treatment of glucagonoma: report of one case].
Nan Fang Yi Ke Da Xue Xue Bao
PUBLISHED: 05-07-2013
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lucagonoma is a rare islet alpha-cell tumor. We report a case of glucagonoma in a 55-year-old male patient with such clinical findings of necrolytic migratory erythema, diabetes mellitus, body weight loss, and anemia. CT examination found a space-occupying lesion in the pancreas, and an elevated serum glucagon level indicate the diagnosis of glucagonoma, which was confirmed postoperatively by pathological examination of the tumor tissue. A definite diagnosis of glucagonoma relies on pathological report, and so far no standard treatment strategy has been available for this tumor. Surgical resection is an effective means for treatment of glucagonoma.
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EGFR promoter methylation detection in cervical cancer by a hybridization-fluorescence polarization assay.
Diagn. Mol. Pathol.
PUBLISHED: 05-01-2013
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The methylation status of the epidermal growth factor receptor (EGFR) promoter is of potential predictive value for benefitting from EGFR inhibition therapy. Stratified therapy assignment for cervical cancer patients based on the EGFR promoter methylation status requires a simple detection method in the daily practice of diagnosis. A novel assay detecting the EGFR promoter methylation status in cervical cancer tissue samples using a hybridization-fluorescence polarization (FP) technique was developed. A pair of primers was used to amplify a 156 bp fragment in the promoter region of EGFR. Two probes specific for either methylated or unmethylated EGFR promoter DNA labeled with different fluorophores hybridized, respectively, with their target amplicons. The EGFR promoter methylation status was determined by the FP values. A total of 273 cervical cancer tissue samples were simultaneously analyzed using the new assay technique and combined bisulfite restriction analysis. The new assay was more sensitive compared with the combined bisulfite restriction analysis, and it allowed the discrimination of the EGFR promoter methylation status directly in solution without the restriction enzyme digestion. Sensitivity, specificity, and stability of the hybridization-FP assay had been recorded. The minimum detection level established with the new assay was 50 copies/?L, and it was able to detect the minor population of the EGFR promoter methylation status even when its contents were as low as 10%. No cross-reaction was observed in the assay when the amount of plasmids used accounted for no more than 10(9) copies/?L. The coefficient of variation of the reproducibility for the assay was <10%.
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Derivation and validation of a prediction score for acute kidney injury in patients hospitalized with acute heart failure in a Chinese cohort.
Nephrology (Carlton)
PUBLISHED: 04-16-2013
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Acute kidney injury (AKI) is a common complication among patients hospitalized for acute heart failure (AHF), and is associated with increased mortality. The goal of this study was to derive and validate a prediction score for AKI in AHF patients.
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JoVE Visualize is a tool created to match the last 5 years of PubMed publications to methods in JoVE's video library.

How does it work?

We use abstracts found on PubMed and match them to JoVE videos to create a list of 10 to 30 related methods videos.

Video X seems to be unrelated to Abstract Y...

In developing our video relationships, we compare around 5 million PubMed articles to our library of over 4,500 methods videos. In some cases the language used in the PubMed abstracts makes matching that content to a JoVE video difficult. In other cases, there happens not to be any content in our video library that is relevant to the topic of a given abstract. In these cases, our algorithms are trying their best to display videos with relevant content, which can sometimes result in matched videos with only a slight relation.