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Find video protocols related to scientific articles indexed in Pubmed.
Genomic organization and expression of immunoglobulin genes in the Chinese hamster (Cricetulus griseus).
Scand. J. Immunol.
PUBLISHED: 10-02-2014
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In science, the hamsters are widely used as a model for studying the human diseases because they display many features like humans. The utility of the chinese hamster as a biology model can be further enhanced by further characterization of the genes encoding components of the immune system. Here, we report the genomic organization and expression of the chinese hamster immunoglobulin heavy and light chain genes. The chinese hamster IgH locus contains 268 VH segments (132 potentially functional genes, 12 ORFs and 124 pseudogenes), 4 DH segments, 6 JH segments, four constant region genes (?, ?, ?, and ?), and one reverse ? remnant fragment. The Ig? locus contains only a single C? gene, 4 J? segments and 48 V? segments (15 potentially functional genes and 33 pseudogenes), whereas the Ig? locus contains 4 C? genes, but only C? 3 and C? 4 each preceded by a J? gene segment. A total of 49 V? segments (39 potentially functional genes, 3 ORFs and 7 pseudogenes) were identified. Analysis of junctions of the recombined V(D)J transcripts reveals complex diversity in both expressed H and ? sequences, but the microhomology-directed VJ recombination obviously results in very limited diversity in the chinese hamster ? gene despite more potential germline encoded combinatorial diversity. This is the first study to make a comprehensive analysis of the Ig genes in the chinese hamster, which provides insights into the Ig genes in placental mammals. This article is protected by copyright. All rights reserved.
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[Expression and regulation of tight junction protein Occludin in nasal polyps].
Zhonghua Er Bi Yan Hou Tou Jing Wai Ke Za Zhi
PUBLISHED: 09-27-2014
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To evaluate the possible role of tight junction protein Occludin in nasal polyps.
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MiR-30a inhibits osteolysis by targeting RunX2 in giant cell tumor of bone.
Biochem. Biophys. Res. Commun.
PUBLISHED: 09-17-2014
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RunX2 has been identified to crucially regulate the osteolysis in giant cell tumor of bone. MiR-30a is an intronic miRNA identified as tumor suppressor, but little is known about its role in giant tumor cell of bone. In our research, we reported miR-30a was down-regulated in GCT whereas RunX2 was highly expressed. Further research proved that miR-30a can regulate the expression of RunX2 by binding to its 3'-UTR, which influence the osteoclast differentiation and osteolysis formation. Thus, these results suggest that miR-30a could directly target RunX2 and participate in osteolysis in GCT.
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[Expression of palate, lung, and nasal epithelial clone in primary and recurrent nasal polyps].
Lin Chung Er Bi Yan Hou Tou Jing Wai Ke Za Zhi
PUBLISHED: 09-16-2014
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To determine the expression of palate, lung, and nasal epithelial clone (PLUNC) in na sal polyps (NP) and evaluate its association with clinical severity.
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Anaerobic treatment of domestic sewage in modified septic tanks at low temperature.
Environ Technol
PUBLISHED: 08-23-2014
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Three laboratory-scale septic tanks, an anaerobic baffled reactor (ABR)-septic tank (R1), a Yuhuan drawing three-dimensional-carrier-septic tank (R2) and a conventional septic tank (R3), were operated in parallel over half a year under hydraulic retention times (HRTs) of 36, 24 and 12 h, with a sewage temperature of 16 degrees C. The removal efficiencies of total chemical oxygen demand (CODtot) achieved in R1 and R2 increased by 14%, 21% and 12% and 18%, 3% and 16%, respectively, under three different HRTs, as compared to those in R3. The total nitrogen and phosphorus removal efficiencies were negligible. R1 sludges had a higher specific methane production rate as compared to that of R2 and R3 sludges. The results indicated that the two modified septic tanks can improve the performance in terms of COD and total solids removal, both were suitable technologies for domestic sewage (pre) treatment at low temperature in northern China.
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Treatment patterns in psoriatic arthritis patients newly initiated on oral nonbiologic or biologic disease-modifying antirheumatic drugs.
Arthritis Res. Ther.
PUBLISHED: 08-22-2014
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This study aimed to describe treatment changes (discontinuation, switching, and therapy add-on) following the initiation of biologic or nonbiologic oral disease-modifying antirheumatic drugs (DMARDs) in psoriatic arthritis (PsA) patients.
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Anti-inflammatory effect of IL-37b in children with allergic rhinitis.
Mediators Inflamm.
PUBLISHED: 08-11-2014
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Interleukin-37 (IL-37), a newly described member of IL-1family, functioned as a fundamental inhibitor of innate inflammatory and immune responses, especially its isoform IL-37b.
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The complete mitochondrial genome of Hylarana guentheri (Amphidia, Anura, Ranidae).
Mitochondrial DNA
PUBLISHED: 08-08-2014
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Abstract The complete mitochondrial genome of Hylarana guentheri was determined. This mitogenome was 19,053?bp in length, containing 13 protein-coding genes, 2 rRNA genes, 22 tRNA genes, and a control region (CR). The following three distinctive features were observed: there was an additional non-coding region of 561?bp between nad5 and nad6; four different tandem repeats were characteristic of the CR region for this species; a pseudogene of tRNA-His (trnH) was found in the CR downstream region.
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Increased Th17 cells and IL?17 in rats with traumatic optic neuropathy.
Mol Med Rep
PUBLISHED: 08-05-2014
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T helper 17 (Th17) cells are strong inducers of numerous autoimmune diseases and inflammation. However, the role of Th17 cells and interleukin (IL)?17 in traumatic optic neuropathy (TON) are yet to be elucidated. In the present study, a rat model of TON was established using a fluid percussion brain injury device. Th17 cells were found to be upregulated in the spleens of rats in the TON group. In addition, the level of IL?17 in the retina of rats in the TON group was observed to increase with the upregulation of the Th17 cells. Furthermore, the expression of IL?17 in the optic nerve was found to be upregulated between one and seven days following injury in the rats in the TON group. These findings strongly suggest that the ratio of Th17 cells and the expression of IL?17 are upregulated in rats with TON. These findings also provide a rationale for developing therapeutic agents to treat TON.
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Comparative phylogeography of two marine species of crustacean: Recent divergence and expansion due to environmental changes.
Gene
PUBLISHED: 08-05-2014
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Environmental changes, such as changes in the coastal topography due to Eurasian plate movements, climate oscillation during the Pleistocene, and alteration of ocean currents, have complicated the geographical structure of marine species and deepened their divergence between populations. As two widely distributed species of crustacean (Oratosquilla oratoria and Eriocheir japonica), weak differences were expected due to their high dispersal potential of planktonic larvae with ocean currents. However, results showed a significant genetic divergence between north of China and south of China in the study. In addition, the estimated north-south divergence time (27-30.5 Myr) of mantis shrimp was near the time of the Himalayan movement, and the China-Japan clade divergence time (10.5-11.9 Myr) of mitten crabs was also coincident with the time of the opening of the Sea of Japan. Thus, we hypothesized that environmental changes in the coastal topography contributed to the marine species divergence. Furthermore, based on phylogenetic analysis, network analysis and haplotype distribution, we surmised that mitten crabs originated from a population with the oldest haplotype (H6) and then divided into the north and south populations due to the recent Eurasian plate movements and ocean currents. And lineage of Japan originated from the north population for the opening of the Sea of Japan. While O. oratoria was guessed to originate from two separate populations in the China Sea. The results of "star-like" network, negative values in neutral test, and Tajima's D statistics of two marine species supported a recent rapid population expansion event after the Pleistocene glaciations.
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The complete mitochondrial genome of Garrulax perspicillatus (Passeriformes, Timaliidae).
Mitochondrial DNA
PUBLISHED: 08-05-2014
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Abstract The complete sequences of the mitochondrial DNA genome from Garrulax perspicillatus was determined by 21 pairs of primers using the polymerase chain reaction method. The genome (17,873?bp in length) contained 37 genes (2 rRNA genes, 22 tRNA genes and 13 protein-coding genes) 2 control regions (CR) at two different locations of mitogenome. All the protein-coding genes in G. perspicillatus were distributed on the H-strand, except for the ND6 subunit gene and eight tRNA genes which were encoded on the L-strand.
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Nonsteroidal anti-inflammatory drug use and the risk of melanoma: a meta-analysis.
Eur. J. Cancer Prev.
PUBLISHED: 07-25-2014
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Nonsteroidal anti-inflammatory drugs (NSAIDs), including aspirin, have emerged as potential chemopreventive agents for melanoma. However, the clinical studies have provided contradictory results as to whether NSAIDs reduce the risk of melanoma. Our aim was to assess this association through a detailed meta-analysis of the studies on the subject published in the peer-reviewed literature. Relevant studies were identified by searching PubMed, EMBASE and Web of Science electronic databases up to July 2012. Reference lists from retrieved articles were also reviewed. Pooled relative risk (RR) estimates and corresponding 95% confidence intervals (CIs) were calculated using the fixed-effects or the random-effects models on the basis of heterogeneity analysis. Subgroup analyses were carried out where data were available. Ten studies involving 490 322 participants contributed to the meta-analysis. The summary RR estimate on the basis of all studies did not indicate that overall NSAIDs use significantly decreases the risk of melanoma (RR=0.94; 95% CI, 0.86-1.03). The use of neither aspirin (RR=0.96; 95% CI, 0.89-1.03) nor nonaspirin NSAIDs (RR=1.05; 95% CI, 0.96-1.14) was associated with the risk of melanoma. Similar results were obtained in the subgroup analyses of cohort studies (RR=1.03; 95% CI, 0.95-1.13), high-intensity NSAID use (the highest dose of NSAID use reported by included studies, RR=1.05; 95% CI, 0.79-1.40), and long-term NSAID use (longest duration of NSAID use reported by included studies, RR=0.87; 95% CI, 0.66-1.14). However, a slight reduction in the risk of melanoma by taking NSAIDs was observed in case-control studies (RR=0.86; 95% CI, 0.80-0.93). In conclusion, the results of our meta-analysis did not indicate that the use of NSAIDs or aspirin is associated with the risk of melanoma. More and in-depth research should focus on those problems in the future.
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Enhanced expression of SAM-pointed domain-containing Ets-like factor in Chronic rhinosinusitis with nasal polyps.
Laryngoscope
PUBLISHED: 07-11-2014
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Chronic rhinosinusitis with nasal polyps (CRSwNP) is characterized by significant goblet hyperplasia and mucus hypersecretion. However, the molecular mechanism underlying mucin overexpression in CRSwNP has not been well characterized. This study sought to assess the expression of SAM-pointed domain-containing Ets-like factor (SPDEF) and its regulation of mucin production in CRSwNP patients.
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A modified surgical procedure for endoscopic optic nerve decompression for the treatment of traumatic optic neuropathy.
N Am J Med Sci
PUBLISHED: 07-10-2014
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Although the endoscopic anterior-to-posterior technique offers many advantages, the long-term effects of the iatrogenic trauma (removal of the uncinate process and anterior ethmoidal sinus) resulting from the complete ethmoidectomy procedure used to gain full access to the optic nerve canal is unknown, and sequelae such as nasal synechia and sinusitis should not be ignored.
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An ethnobotanical study of the less known wild edible figs (genus Ficus) native to Xishuangbanna, Southwest China.
J Ethnobiol Ethnomed
PUBLISHED: 07-08-2014
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The genus Ficus, collectively known as figs, is a key component of tropical forests and is well known for its ethnobotanical importance. In recent decades an increasing number of studies have shown the indigenous knowledge about wild edible Ficus species and their culinary or medicinal value. However, rather little is known about the role of these species in rural livelihoods, because of both species and cultural diversity.
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Factors Driving the Adoption of Quality Improvement Initiatives in Local Health Departments: Results From the 2010 Profile Study.
J Public Health Manag Pract
PUBLISHED: 07-01-2014
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Over the past decade, quality improvement (QI) has become a major focus in advancing the goal of improving performance of local health departments (LHDs). However, limited empirical data exists on the current implementation of QI initiatives in LHDs and factors associated with adoption of QI initiatives.
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Vampire bats exhibit evolutionary reduction of bitter taste receptor genes common to other bats.
Proc. Biol. Sci.
PUBLISHED: 06-27-2014
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The bitter taste serves as an important natural defence against the ingestion of poisonous foods and is thus believed to be indispensable in animals. However, vampire bats are obligate blood feeders that show a reduced behavioural response towards bitter-tasting compounds. To test whether bitter taste receptor genes (T2Rs) have been relaxed from selective constraint in vampire bats, we sampled all three vampire bat species and 11 non-vampire bats, and sequenced nine one-to-one orthologous T2Rs that are assumed to be functionally conserved in all bats. We generated 85 T2R sequences and found that vampire bats have a significantly greater percentage of pseudogenes than other bats. These results strongly suggest a relaxation of selective constraint and a reduction of bitter taste function in vampire bats. We also found that vampire bats retain many intact T2Rs, and that the taste signalling pathway gene Calhm1 remains complete and intact with strong functional constraint. These results suggest the presence of some bitter taste function in vampire bats, although it is not likely to play a major role in food selection. Together, our study suggests that the evolutionary reduction of bitter taste function in animals is more pervasive than previously believed, and highlights the importance of extra-oral functions of taste receptor genes.
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Evaluation of airway hyperresponsiveness in chronic rhinosinusitis: values of sinus computed tomography.
Ann. Allergy Asthma Immunol.
PUBLISHED: 06-20-2014
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Chronic rhinosinusitis (CRS) is tightly linked to airway hyperresponsiveness (AHR) and asthma. However, the practical surrogate parameters for evaluating AHR in patients with CRS remain unclear.
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Rapid evolution of piRNA pathway in the teleost fish: implication for an adaptation to transposon diversity.
Genome Biol Evol
PUBLISHED: 05-22-2014
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The Piwi-interacting RNA (piRNA) pathway is responsible for germline specification, gametogenesis, transposon silencing, and genome integrity. Transposable elements can disrupt genome and its functions. However, piRNA pathway evolution and its adaptation to transposon diversity in the teleost fish remain unknown. This article unveils evolutionary scene of piRNA pathway and its association with diverse transposons by systematically comparative analysis on diverse teleost fish genomes. Selective pressure analysis on piRNA pathway and miRNA/siRNA (microRNA/small interfering RNA) pathway genes between teleosts and mammals showed an accelerated evolution of piRNA pathway genes in the teleost lineages, and positive selection on functional PAZ (Piwi/Ago/Zwille) and Tudor domains involved in the Piwi-piRNA/Tudor interaction, suggesting that the amino acid substitutions are adaptive to their functions in piRNA pathway in the teleost fish species. Notably five piRNA pathway genes evolved faster in the swamp eel, a kind of protogynous hermaphrodite fish, than the other teleosts, indicating a differential evolution of piRNA pathway between the swamp eel and other gonochoristic fishes. In addition, genome-wide analysis showed higher diversity of transposons in the teleost fish species compared with mammals. Our results suggest that rapidly evolved piRNA pathway in the teleost fish is likely to be involved in the adaption to transposon diversity.
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Massive losses of taste receptor genes in toothed and baleen whales.
Genome Biol Evol
PUBLISHED: 05-08-2014
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Taste receptor genes are functionally important in animals, with a surprising exception in the bottlenose dolphin, which shows extensive losses of sweet, umami, and bitter taste receptor genes. To examine the generality of taste gene loss, we examined seven toothed whales and five baleen whales and sequenced the complete repertoire of three sweet/umami (T1Rs) and ten bitter (T2Rs) taste receptor genes. We found all amplified T1Rs and T2Rs to be pseudogenes in all 12 whales, with a shared premature stop codon in 10 of the 13 genes, which demonstrated massive losses of taste receptor genes in the common ancestor of whales. Furthermore, we analyzed three genome sequences from two toothed whales and one baleen whale and found that the sour taste marker gene Pkd2l1 is a pseudogene, whereas the candidate salty taste receptor genes are intact and putatively functional. Additionally, we examined three genes that are responsible for taste signal transduction and found the relaxation of functional constraints on taste signaling pathways along the ancestral branch leading to whales. Together, our results strongly suggest extensive losses of sweet, umami, bitter, and sour tastes in whales, and the relaxation of taste function most likely arose in the common ancestor of whales between 36 and 53 Ma. Therefore, whales represent the first animal group to lack four of five primary tastes, probably driven by the marine environment with high concentration of sodium, the feeding behavior of swallowing prey whole, and the dietary switch from plants to meat in the whale ancestor.
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[Clinical effect of clarithromycin therapy in patients with chronic rhinosinusitis].
Zhonghua Er Bi Yan Hou Tou Jing Wai Ke Za Zhi
PUBLISHED: 04-19-2014
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To evaluate the efficacy of clarithromycin (CAM) treatment in adult Chinese patients suffering from chronic rhinosinusitis with nasal polyps (CRSwNP) or without nasal polyps (CRSsNP).
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Azelastine enhances the clinical efficacy of glucocorticoid by modulating MKP-1 expression in allergic rhinitis.
Eur Arch Otorhinolaryngol
PUBLISHED: 04-17-2014
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Azelastine was suggested as a supplementary choice of glucocorticoid for the control of moderate to severe allergic rhinitis (AR). However, the underlying mechanism has not been completely understood. In this study, primary cultured nasal epithelial cells and bronchial epithelial cells were stimulated with proinflammatory cytokines (IL-1? and IL-17A) and anti-inflammatory agents (azelastine and budesonide) in vitro. The expression of intercellular adhesion molecule 1 (ICAM-1) and mitogen-activated protein kinase phosphatase-1 (MKP-1) was examined using qPCR and ELISA, respectively. Moreover, the additive effects of azelastine and budesonide nasal spray on nasal ICAM-1 level and total nasal symptom scores were evaluated in six uncontrolled severe AR patients by budesonide nasal spray alone. We found azelastine significantly inhibited cytokine-induced ICAM-1 upregulation, which is reversed by MKP-1 silencing. Azelastine and budesonide additively increased MKP-1 expression and inhibited ICAM-1 expression in vitro. After treatment for two consecutive weeks, combined azelastine and budesonide nasal spray significantly decreased nasal ICAM-1 level and TNSS in six uncontrolled AR patients. Our findings suggested that azelastine is able to additively enhance the anti-inflammatory effect of budesonide by modulating MKP-1 expression, which may implicate in the treatment of uncontrolled severe AR.
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Design, synthesis and evaluation of phenethylaminoheterocycles as K(v)1.5 inhibitors.
Bioorg. Med. Chem. Lett.
PUBLISHED: 04-17-2014
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Phenethylaminoheterocycles have been prepared and assayed for inhibition of the Kv1.5 potassium ion channel as a potential approach to the treatment of atrial fibrillation. A diverse set of heterocycles were identified as potent Kv1.5 inhibitors and were advanced to pharmacodynamic evaluation based on selectivity and pharmacokinetic profile. Heterocycle optimization and template modification lead to the identification of compound 24 which demonstrated increased atrial effective refractory period in the rabbit pharmacodynamic model with mild effects on blood pressure and heart rate.
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Identification and characterization of genes differentially expressed in X and Y sperm using suppression subtractive hybridization and cDNA microarray.
Mol. Reprod. Dev.
PUBLISHED: 04-15-2014
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Differential expression of genes leads to variations in the phenotypes of X and Y sperm, although some differentially expressed gene products are shared through intercellular bridges. Genes differentially expressed in bovine X and Y sperm were identified by a combination of suppression subtractive hybridization (SSH), cDNA microarray, and sequence-homology analysis. Microarray data and Significance Analysis of Microarrays software were used to identify 31 differentially expressed genes, only four of which were previously identified. These genes are involved in fundamental life processes of mature sperm, and may be associated with the differences between X and Y sperm since 27 versus 4 were upregulated in X versus Y sperm, respectively. The levels of expression of seven genes-including the known genes UTY, DPH3, CYTB, and ISCU, and the unknown genes X?+?Y contig 41, X?+?Y contig 18, and Y?+?X contig 16-were validated by quantitative real-time PCR, and some genes were clearly differentially expressed by X and Y sperm, despite the presence of intercellular bridges among spermatids. These results provide a theoretical basis for research on gene expression during sperm development, as well as on sex control at the level of sperm. Mol. Reprod. Dev. 81: 908-917, 2014. © 2014 Wiley Periodicals, Inc.
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N,N'-Bis[3,5-bis(trifluoromethyl)phenyl]thiourea: a privileged motif for catalyst development.
Org. Biomol. Chem.
PUBLISHED: 04-08-2014
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Over the last decade, the use of (thio)urea derivatives as organocatalysts in organic chemistry has increased rapidly. One of the key features is their ability to activate substrates and subsequently stabilize partially developing negative charges (e.g., oxyanions) in the transition states employing explicit double hydrogen bonding. Among (thio)urea-based catalysts, N,N'-bis[3,5-bis(trifluoromethyl)phenyl]thiourea developed by Schreiner's group (abbreviated here as Schreiner's thiourea) has played a very important role in the development of H-bond organocatalysts. Nowadays it is used extensively in promoting organic transformations, and the 3,5-bis(trifluoromethyl)phenyl motif thereof is used ubiquitously in H-bond catalysts. This review summarizes the key developments of Schreiner's thiourea-mediated reactions with the aim to further expand the applications of (thio)urea-based catalysts.
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Endoscopic optic nerve decompression for patients with traumatic optic neuropathy: is nerve sheath incision necessary?
ORL J. Otorhinolaryngol. Relat. Spec.
PUBLISHED: 04-03-2014
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To evaluate the necessity of nerve sheath incision for the treatment of patients with traumatic optic neuropathy (TON) during endoscopic optic nerve decompression.
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Improved production, purification and bioactivity of a polysaccharide from submerged cultured Ganoderma lucidum.
Arch. Pharm. Res.
PUBLISHED: 04-02-2014
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Polysaccharides from Ganoderma lucidum showed multiple biological activities, such as immuno-modulating, antitumor, antioxidant, and hepatoprotective activity, etc. Adlay oil was added into the media to enhance polysaccharide production by submerged culture of G. lucidum in this work. The results revealed the optimal concentration of adlay oil was 1.5 % for polysaccharide production of G. lucidum. Analysis of the polysaccharide components confirmed that no novel components were biosynthesized by the addition of adlay oil. The main fraction of extracellular polysaccharide, GLEP-2, was isolated from the submerged culture broth of G. lucidum by ethanol precipitation, filtration, DEAE cellulose-52 and Sepharose CL-6B chromatography. GLEP-2, which was composed of glucose, galactose, mannose, arabinose, and rhamnose in a ratio of 332:55:32:13:3 respectively, had an average molecular weight of ~2.08 × 10(4) Da. The bioactivity tests demonstrated that GLEP-2 enhanced the T lymphocyte proliferation significantly at a concentration of 200 ?g/mL and B lymphocyte proliferation at lower concentrations of 50 ?g/mL. The results suggest polysaccharides from the submerged culture of G. lucidum are potential candidates for further development and possible commercial applications, especially in the pharmaceutical and functional foods industries.
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Diffusion-weighted magnetic resonance imaging to evaluate microvascular density after transarterial embolization ablation in a rabbit VX2 liver tumor model.
Magn Reson Imaging
PUBLISHED: 03-25-2014
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To evaluate the correlation between findings from diffusion weighted imaging (DWI) and microvascular density (MVD) measurements in VX2 liver tumors after transarterial embolization ablation (TEA).
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One of the distinctive properties of ionic liquids over molecular solvents and inorganic salts: enhanced basicity stemming from the electrostatic environment and "free" microstructure.
J Phys Chem B
PUBLISHED: 03-24-2014
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The basicity of ionic liquids (ILs) underlies many important IL-based processes including the dissolution and conversion of cellulose, the capture of CO2, and metal catalysis. In this work, we have disclosed the nature of the basicity of ILs, i.e., the difference between the basicity of IL and the basicity of the molecular solvent and inorganic salt, through a quantitative electrostatic and electronic analysis of the molecular surface for the first time. The results reveal one of the distinctive properties of ILs (enhanced basicity over molecular solvents and inorganic salts with the same basic site) stemming from their special electrostatic environment and microstructure. The enhancement is significant, from either the electrostatic aspect or the covalent aspect of basicity. The peculiar electrostatic environment of ILs leads to stronger basicity than similar molecular solvents, and the relatively freer microstructure of ILs contributes to the enhancement of basicity over their inorganic analogues. These results are highly instructive for better understanding the unique value of ILs and designing novel ILs to improve the efficiency of basicity-related processes.
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MiR-126-5p regulates osteolysis formation and stromal cell proliferation in giant cell tumor through inhibition of PTHrP.
Bone
PUBLISHED: 03-18-2014
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Parathyroid hormone-related protein (PTHrP) has been identified to play a crucial role in osteolysis formation and stromal cell (GCTSC) proliferation in giant cell tumor (GCT). MiR-126-5p is an intronic miRNA identified as tumor suppressor in many tumors, but its role in GCT is poorly understood. We found that miR-126-5p was decreased in GCT and could directly regulate PTHrP expression. Furthermore, miR-126-5p could control osteoclast (OC) differentiation, GCTSC proliferation and osteolysis formation in GCT through negative regulation of PTHrP. Thus, these results suggest that miR-126-5p could directly target PTHrP and have a tumor suppressor function in GCT.
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[Effect of occupational stress on oxidation/antioxidant capacity in nurses].
Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi
PUBLISHED: 03-18-2014
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To investigate the effect of occupational stress on the oxidation/antioxidant capacity in nurses.
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[Analysis of correlation between occupational stress and serum monoamine neurotransmitters in nurses].
Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi
PUBLISHED: 03-18-2014
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To investigate the impact of occupational stress on serum monoamine neurotransmitters in nurses.
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Racial/Ethnic Disparities in Preventive Care Practice Among U.S. Nursing Home Residents.
J Aging Health
PUBLISHED: 03-13-2014
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To assess racial/ethnic disparities in preventive care practices among U.S. nursing home residents.
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The discovery of asunaprevir (BMS-650032), an orally efficacious NS3 protease inhibitor for the treatment of hepatitis C virus infection.
J. Med. Chem.
PUBLISHED: 03-05-2014
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The discovery of asunaprevir (BMS-650032, 24) is described. This tripeptidic acylsulfonamide inhibitor of the NS3/4A enzyme is currently in phase III clinical trials for the treatment of hepatitis C virus infection. The discovery of 24 was enabled by employing an isolated rabbit heart model to screen for the cardiovascular (CV) liabilities (changes to HR and SNRT) that were responsible for the discontinuation of an earlier lead from this chemical series, BMS-605339 (1), from clinical trials. The structure-activity relationships (SARs) developed with respect to CV effects established that small structural changes to the P2* subsite of the molecule had a significant impact on the CV profile of a given compound. The antiviral activity, preclincial PK profile, and toxicology studies in rat and dog supported clinical development of BMS-650032 (24).
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Development of a neutralizing mouse-pig chimeric antibody with therapeutic potential against Haemophilus parasuis in Pichia pastoris.
FEMS Microbiol. Lett.
PUBLISHED: 03-02-2014
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Haemophilus parasuis is one of the most important bacterial diseases of pigs worldwide. The lack of a vaccine against a broad spectrum of strains and the limitation of antimicrobial susceptibility hamper the control of disease. In this study, we cloned the constant regions of gamma heavy chains and kappa light chain of pig lymphocytes in frame with the variable regions of heavy and light chains of mouse monoclonal antibody 1D8, which reacts with all 15 serotypes of H. parasuis and has neutralizing activity. The constructed mouse-pig chimeric antibody was expressed in Pichia pastoris. Results demonstrated that the expressed chimeric antibody inhibited the growth of H. parasuis in vitro. Furthermore, the experiments in mice showed that chimeric antibody increased survival rate of the mice compared with that of the control group (P < 0.05). Importantly, the chimeric antibody partially protected piglets against H. parasuis infection according to the clinical lesion scores and PCR results of H. parasuis in the tissues from piglets of the chimeric antibody-inoculated group and the PBS group. In summary, our results demonstrated that the mouse-pig chimeric antibody could be a therapeutic candidate to prevent the H. parasuis infection and control the prevalence of disease.
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Prognostic factors of patients with spinal chondrosarcoma: a retrospective analysis of 98 consecutive patients in a single center.
Ann. Surg. Oncol.
PUBLISHED: 02-21-2014
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Chondrosarcoma (CHS) in the spine is relatively rare and minimal information has been published in the literature regarding this subject. The objective of our study was to discuss the factors that may affect outcomes of patients with spinal CHS.
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Surgery and outcomes of six patients with intradural epidermoid cysts in the lumbar spine.
World J Surg Oncol
PUBLISHED: 02-19-2014
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This was a retrospective study.
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The complete mitochondrial genome of Amolops ricketti (Amphidia, Anura, Ranidae).
Mitochondrial DNA
PUBLISHED: 02-14-2014
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Abstract The complete mitochondrial genome from the South China torrent frog Amolops ricketti was determined. This mitogenome was 17,771?bp in length, containing 13 protein-coding genes, 2 rRNA genes, 22 tRNA genes and a control region (CR). All the protein-coding genes in A. ricketti were distributed on the H-strand, except for the ND6 subunit gene and eight tRNA genes which were encoded on the L-strand.
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The G??-Src signaling pathway regulates TNF-induced necroptosis via control of necrosome translocation.
Cell Res.
PUBLISHED: 02-11-2014
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Formation of multi-component signaling complex necrosomes is essential for tumor necrosis factor ? (TNF)-induced programmed necrosis (also called necroptosis). However, the mechanisms of necroptosis are still largely unknown. We isolated a TNF-resistant L929 mutant cell line generated by retrovirus insertion and identified that disruption of the guanine nucleotide-binding protein ? 10 (G?10) gene is responsible for this phenotype. We further show that G?10 is involved in TNF-induced necroptosis and G?2 is the partner of G?10. Src is the downstream effector of G?2?10 in TNF-induced necroptosis because TNF-induced Src activation was impaired upon G?10 knockdown. G?10 does not affect TNF-induced activation of NF-?B and MAPKs and the formation of necrosomes, but is required for trafficking of necrosomes to their potential functioning site, an unidentified subcellular organelle that can be fractionated into heterotypic membrane fractions. The TNF-induced G??-Src signaling pathway is independent of RIP1/RIP3 kinase activity and necrosome formation, but is required for the necrosome to function.
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The complete mitochondrial genome of Garrulax cineraceus (Aves, Passeriformes, Timaliidae).
Mitochondrial DNA
PUBLISHED: 01-24-2014
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Abstract The complete sequence of the mitochondrial DNA genome from Garrulax cineraceus was determined using the polymerase chain reaction method. The genome (17,800?bp in length) contained 37 genes (13 protein-coding genes, 2 rRNA genes and 22 tRNA genes) and 2 control regions (D-loop) at two different locations of mitogenome, which is similar to the typical mtDNA of vertebrates. All the protein-coding genes in G. cineraceus were distributed on the H-strand, except for the ND6 subunit gene and eight tRNA genes which were encoded on the L-strand.
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Clinical characteristics and surrogate markers of eosinophilic chronic rhinosinusitis in Southern China.
Eur Arch Otorhinolaryngol
PUBLISHED: 01-22-2014
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The surrogate markers for subclassifying eosinophilic chronic rhinosinusitis (ECRS) and non-ECRS remain elusive. We herein performed a cross-sectional study to assess the clinical implication of clinical symptoms, CT findings, blood eosinophil (EOS) examination based on histological examination of tissue eosinophilia in 105 adult CRS patients (including 72 with nasal polyps and 33 without nasal polyps) in southern China. We found the mean score of smell loss was significantly higher in ECRS subgroup than those in non-ECRS subgroup (p < 0.05), whereas the average ethmoid osteitis index in non-ECRS subgroup was significantly higher than that in ECRS subgroup (p < 0.05). Moreover, we found both the mean blood EOS number and ratio were significantly higher in ECRS subgroup than those in non-ECRS subgroup (p < 0.05). By applying receiver operating characteristic (ROC) curve analysis, we found blood EOS number had a sensitivity of 84.9 % and specificity of 84.4 % [area under the curve (AUC): 0.873] at the cutoff level of 0.16 × 10(9)/L, and blood EOS ratio had a sensitivity of 89.0 % and specificity of 84.4 % (AUC: 0.863) at the cutoff level of 2.05 % in this cohort. Our findings indicated that smell loss score, ethmoid osteitis index and blood EOS number and ratio may be used for the differential diagnosis of ECRS as the surrogate markers.
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Opiorphin increases blood pressure of conscious rats through renin-angiotensin system (RAS).
Peptides
PUBLISHED: 01-22-2014
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Human opiorphin is a recently identified endogenous pentapeptide, encoded by ProL1 multigenes family that contributes to cardiovascular modulation. The aim of this study was to evaluate the effect of opiorphin through intravenous injection (i.v.) on mean arterial pressure (MAP) regulation. To investigate the bioactivity of opiorphin, a rat cannulation model was developed for MAP measurement and blood sampling. In our present study, opiorphin (200-700 nmol/kg) increased MAP in dose-related and time-dependent manner in conscious rats, which associated highly with the elevation of angiotensin II (AngII) levels in serum. Furthermore, the MAP elevation induced by opiorphin was completely blocked by AngII receptor antagonist valsartan and partially attenuated by angiotensin-converting enzyme inhibitor captopril. Finally, we tested the effect of opiorphin in hypoxia condition, which exhibited that opiorphin reversed hypoxia induced hypotension in conscious rats. Taken together, these results indicated that opiorphin may play an important role in the modulation of blood pressure through AngII dependent pathway, which may help future development of potent clinical therapeutics for emergency treatment.
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Evolution of mitochondrial DNA and its relation to basal metabolic rate.
Mitochondrial DNA
PUBLISHED: 01-21-2014
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Abstract Energy metabolism is essential for the survival of animals, which can be characterized by maximum metabolic rate (MMR) and basal metabolic rate (BMR). Because of the crucial roles of mitochondria in energy metabolism, mitochondrial DNA (mtDNA) has been subjected to stronger purifying selection in strongly locomotive than weakly locomotive birds and mammals. Although maximum locomotive speed (an indicator of MMR) showed a negative correlation with the evolutionary rate of mtDNA, it is unclear whether BMR has driven the evolution of mtDNA. Here, we take advantage of the large amount of mtDNA and BMR data in 106 mammals to test whether BMR has influenced the mtDNA evolution. Our results showed that, in addition to the locomotive speed, mammals with higher BMR have subjected to stronger purifying selection on mtDNA than did those with lower BMR. The evolution of mammalian mtDNA has been modified by two levels of energy metabolism, including MMR and BMR. Our study provides a more comprehensive view of mtDNA evolution in relation to energy metabolism.
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The complete mitochondrial genome of Microhyla pulchra (Amphidia, Anura, Microhylidae).
Mitochondrial DNA
PUBLISHED: 01-21-2014
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Abstract The complete mitochondrial genome of Microhyl pulchra was determined in this work. This mitogenome was 16,744?bp in length, containing 13 protein-coding genes, 2 rRNA genes, 22 tRNA genes and a control region (CR). The following four distinctive features were observed: a protein-coding gene (ND1) began with GTG as start codon; eight protein-coding genes (ND1, COII, ATP6, COIII, ND3, ND4, ND5 and Cytb) ended with incomplete stop codon T; four tRNA genes positions (tRNA-Leu (CUN)/tRNA-Thr/Trna-Pro/tRNA-Phe) located between CR and 12S rRNA genes, which was a novel mtDNA gene rearrangement in amphibians; there was no significant repeat regions in the CR.
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Ambient lithium-SO2 batteries with ionic liquids as electrolytes.
Angew. Chem. Int. Ed. Engl.
PUBLISHED: 01-20-2014
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Li-SO2 batteries have a high energy density but bear serious safety problems that are associated with pressurized SO2 and flammable solvents in the system. Herein, a novel ambient Li-SO2 battery was developed through the introduction of ionic liquid (IL) electrolytes with tailored basicities to solvate SO2 by reversible chemical absorption. By tuning the interactions of ILs with SO2, a high energy density and good discharge performance with operating voltages above 2.8?V were obtained. This strategy based on reversible chemical absorption of SO2 in IL electrolytes enables the development of the next generation of ambient Li-SO2 batteries.
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Ambipolar MoTe2 transistors and their applications in logic circuits.
Adv. Mater. Weinheim
PUBLISHED: 01-17-2014
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We report ambipolar charge transport in ?-molybdenum ditelluride (MoTe2 ) flakes, whereby the temperature dependence of the electrical characteristics was systematically analyzed. The ambipolarity of the charge transport originated from the formation of Schottky barriers at the metal/MoTe2 contacts. The Schottky barrier heights as well as the current on/off ratio could be modified by modulating the electrostatic fields of the back-gate voltage (Vbg) and drain-source voltage (Vds). Using these ambipolar MoTe2 transistors we fabricated complementary inverters and amplifiers, demonstrating their feasibility for future digital and analog circuit applications.
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Transcriptome sequencing and phylogenomic resolution within Spalacidae (Rodentia).
BMC Genomics
PUBLISHED: 01-14-2014
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Subterranean mammals have been of great interest for evolutionary biologists because of their highly specialized traits for the life underground. Owing to the convergence of morphological traits and the incongruence of molecular evidence, the phylogenetic relationships among three subfamilies Myospalacinae (zokors), Spalacinae (blind mole rats) and Rhizomyinae (bamboo rats) within the family Spalacidae remain unresolved. Here, we performed de novo transcriptome sequencing of four RNA-seq libraries prepared from brain and liver tissues of a plateau zokor (Eospalax baileyi) and a hoary bamboo rat (Rhizomys pruinosus), and analyzed the transcriptome sequences alongside a published transcriptome of the Middle East blind mole rat (Spalax galili). We characterize the transcriptome assemblies of the two spalacids, and recover the phylogeny of the three subfamilies using a phylogenomic approach.
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The complete mitochondrial genome of Paradoxornis webbianus (Passeriformes, Muscicapidae).
Mitochondrial DNA
PUBLISHED: 01-14-2014
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Abstract The complete sequences of the mitochondrial DNA genome from Paradoxornis webbianus was determined using the polymerase chain reaction method. The genome (16,960?bp in length) contained 37 genes (13 protein-coding genes, 2 rRNA genes and 22 tRNA genes), a control region (D-loop) and a non-coding region at two different locations of mitogenome, which is similar to the typical mtDNA of vertebrates. All the protein-coding genes in P. webbianus were distributed on the H-strand, except for the ND6 subunit gene and eight tRNA genes which were encoded on the L-strand.
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Enhancing the basicity of ionic liquids by tuning the cation-anion interaction strength and via the anion-tethered strategy.
J Phys Chem B
PUBLISHED: 01-14-2014
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Ionic liquids (ILs) with relatively strong basicity often show impressive performance in chemical processes, so it is important to enhance the basicity of ILs by molecular design. Here, we proposed two effective ways to enhance the basicity of ILs: by weakening the cation-anion interaction strength and by employing the anion-tethered strategy. Notably, two quantum-chemical parameters, the most negative surface electrostatic potential and the lowest surface average local ionization energy, were adopted as powerful tools to demonstrate the electrostatic and covalent aspects of basicity, respectively, at the microscopic level. It was shown that, for the ILs with the same anion (acetate or trifluoroacetate), the basicity of the ILs could be enhanced when the cation-anion interaction strength was weakened. For the acetate anion-based ILs, the hydrogen-bonding basicity scale (?) increased by 29% when the cation changed from 1-butyl-3-methylimidazolium ([Bmim]) to tetrabutylphosphonium ([P4444]), achieving one of the highest reported ? values for ILs. Moreover, it was also demonstrated that, when an amine group was tethered to the anion of the IL, its basicity was stronger than when it was tethered to the cation. These results are highly instructive for designing ILs with strong basicity and for improving the efficiency of IL-based processes, such as CO2 capture, SO2 and acetylene absorption, dissolution of cellulose, extraction of bioactive compounds, and so on.
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Use acupuncture to relieve perimenopausal syndrome: study protocol of a randomized controlled trial.
Trials
PUBLISHED: 01-09-2014
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Whether acupuncture is effective for relieving perimenopausal syndrome has been controversial recently. In this article, we report the protocol of a randomized controlled trial using acupuncture to treat perimenopausal syndrome, aiming to answer this controversy.
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A promising gene delivery system developed from PEGylated MoS2 nanosheets for gene therapy.
Nanoscale Res Lett
PUBLISHED: 01-01-2014
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A new class of two-dimensional (2D) nanomaterial, transition metal dichalcogenides (TMDCs) such as MoS2, MoSe2, WS2, and WSe2 which have fantastic physical and chemical properties, has drawn tremendous attention in different fields recently. Herein, we for the first time take advantage of the great potential of MoS2 with well-engineered surface as a novel type of 2D nanocarriers for gene delivery and therapy of cancer. In our system, positively charged MoS2-PEG-PEI is synthesized with lipoic acid-modified polyethylene glycol (LA-PEG) and branched polyethylenimine (PEI). The amino end of positively charged nanomaterials can bind to the negatively charged small interfering RNA (siRNA). After detection of physical and chemical characteristics of the nanomaterial, cell toxicity was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Polo-like kinase 1 (PLK1) was investigated as a well-known oncogene, which was a critical regulator of cell cycle transmission at multiple levels. Through knockdown of PLK1 with siRNA carried by novel nanovector, qPCR and Western blot were used to measure the interfering efficiency; apoptosis assay was used to detect the transfection effect of PLK1. All results showed that the novel nanocarrier revealed good biocompatibility, reduced cytotoxicity, as well as high gene-carrying ability without serum interference, thus would have great potential for gene delivery and therapy.
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Expression of intracellular interferon-alpha confers antiviral properties in transfected bovine fetal fibroblasts and does not affect the full development of SCNT embryos.
PLoS ONE
PUBLISHED: 01-01-2014
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Foot-and-mouth disease, one of the most significant diseases of dairy herds, has substantial effects on farm economics, and currently, disease control measures are limited. In this study, we constructed a vector with a human interferon-? (hIFN-?) (without secretory signal sequence) gene cassette containing the immediate early promoter of human cytomegalovirus. Stably transfected bovine fetal fibroblasts were obtained by G418 selection, and hIFN-? transgenic embryos were produced by somatic cell nuclear transfer (SCNT). Forty-six transgenic embryos were transplanted into surrogate cows, and five cows (10.9%) became pregnant. Two male cloned calves were born. Expression of hIFN-? was detected in transfected bovine fetal fibroblasts, transgenic SCNT embryos, and different tissues from a transgenic SCNT calf at two days old. In transfected bovine fetal fibroblasts, expression of intracellular IFN-? induced resistance to vesicular stomatitis virus infection, increased apoptosis, and induced the expression of double-stranded RNA-activated protein kinase gene (PKR) and the 2'-5'-oligoadenylate synthetase gene (2'-5' OAS), which are IFN-inducible genes with antiviral activity. Analysis by qRT-PCR showed that the mRNA expression levels of PKR, 2'-5' OAS, and P53 were significantly increased in wild-type bovine fetal fibroblasts stimulated with extracellular recombinant human IFN-?-2b, showing that intracellular IFN-? induces biological functions similar to extracellular IFN-?. In conclusion, expression of intracellular hIFN-? conferred antiviral properties in transfected bovine fetal fibroblasts and did not significantly affect the full development of SCNT embryos. Thus, IFN-? transgenic technology may provide a revolutionary way to achieve elite breeding of livestock.
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Interleukin-17A promotes MUC5AC expression and goblet cell hyperplasia in nasal polyps via the Act1-mediated pathway.
PLoS ONE
PUBLISHED: 01-01-2014
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Recent studies demonstrated that nasal polyps (NP) patients in China and other Asian regions possessed distinct Th17-dominant inflammation and enhanced tissue remodeling. However, the mechanism underlying these observations is not fully understood. This study sought to evaluate the association of interleukin (IL)-17A with MUC5AC expression and goblet cell hyperplasia in Chinese NP patients and to characterize the signaling pathway underlying IL-17A-induced MUC5AC expression in vitro.
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Association of radiologic findings with mortality in patients with avian influenza H7N9 pneumonia.
PLoS ONE
PUBLISHED: 01-01-2014
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The novel H7N9 virus causes severe illness, including pneumonia and acute respiratory distress syndrome, with high rates of mortality. We investigated the association of initial radiologic characteristics obtained at admission with clinical outcomes in patients with avian influenza H7N9 pneumonia.
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Broncho-Vaxom attenuates allergic airway inflammation by restoring GSK3?-related T regulatory cell insufficiency.
PLoS ONE
PUBLISHED: 01-01-2014
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Oral administration of bacterial extracts (eg, Broncho-Vaxom (BV)) has been proposed to attenuate asthma through modulating Treg cells. However, the underlying mechanism has not been fully characterized. This study sought to assess the effects of oral administration of BV on GSK-3? expression and Treg cells in ovalbumin (OVA)-induced asthmatic mice models.
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Cluster subcutaneous allergen specific immunotherapy for the treatment of allergic rhinitis: a systematic review and meta-analysis.
PLoS ONE
PUBLISHED: 01-01-2014
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Although allergen specific immunotherapy (SIT) represents the only immune- modifying and curative option available for patients with allergic rhinitis (AR), the optimal schedule for specific subcutaneous immunotherapy (SCIT) is still unknown. The objective of this study is to systematically assess the efficacy and safety of cluster SCIT for patients with AR.
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Variation in delivery of the 10 essential public health services by local health departments for obesity control in 2005 and 2008.
J Public Health Manag Pract
PUBLISHED: 12-18-2013
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To describe and compare the capacity of local health departments (LHDs) to perform 10 essential public health services (EPHS) for obesity control in 2005 and 2008, and explore factors associated with provision of these services.
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MiR-126-5p regulates osteoclast differentiation and bone resorption in giant cell tumor through inhibition of MMP-13.
Biochem. Biophys. Res. Commun.
PUBLISHED: 12-11-2013
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Giant cell tumor (GCT) of bone is an aggressive skeletal tumor characterized by localized bone resorption. Matrix metalloproteinase-13 (MMP-13) is the principal proteinase expressed by the stromal cells of GCT (GCTSCs) and also considered to play a crucial role in formation of the osteolytic lesion in GCT. However, the exact mechanism of the regulation of MMP-13 expression in GCTSCs was unknown. In this study, we identified miR-126-5p was significantly downregulated in GCTSCs and affect osteoclast (OC) differentiation and bone resorption by repressing MMP-13 expression at the post-transcriptional level. Thus, our studies show that miR-126-5p plays an important physiological role in multinucleated giant cell formation and osteolytic lesion in GCT.
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Sequence and organization of complete mitochondrial genome of the firefly, Aquatica leii (Coleoptera: Lampyridae).
Mitochondrial DNA
PUBLISHED: 12-03-2013
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Abstract The firefly Aquatica leii (Coleoptera: Lampyridae) is widely distributed in China. In this study, we sequenced and characterized the first complete mitochondrial genome of the firefly from the subfamily Luciolinae. The circular genome of 16,856?bp in length contains 13 protein-coding genes, 22 transfer RNA genes, 2 ribosomal RNA genes and a non-coding AT-rich region. Overall base composition of the genome is 42.28% A, 34.80% T, 13.91% C and 9.01% G, with an AT bias of 77.08%. All protein-coding genes start with an ATN codon, and terminate with the typical stop codon TAA, TAG or a single T. The non-coding AT-rich region is unusually long (2239?bp), containing six 113?bp tandem repeats and a microsatellite-like (TA)7 element. The genome sequence is useful for studying the evolution of sexual signaling and many ecological specializations in fireflies.
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A hospital-based survey on the prevalence of bronchial asthma in patients with allergic rhinitis in southern China.
Am J Rhinol Allergy
PUBLISHED: 11-27-2013
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The prevalence of bronchial asthma (BA) and the underlying risk factors in patients with allergic rhinitis (AR) in China are largely unknown. Thus, this study is designed to assess the BA comorbidity in AR patients in two cities (Guangzhou and Zhuhai) of southern China and to determine the risk factors of BA in these AR patients.
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Characterization of complete mitochondrial genome of the skipper butterfly, Celaenorrhinus maculosus (Lepidoptera: Hesperiidae).
Mitochondrial DNA
PUBLISHED: 10-10-2013
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Abstract The skipper butterfly, Celaenorrhinus maculosus (Lepidoptera: Hesperiidae), is a small butterfly species that is widely distributed in Taiwan and mainland China. In this work, we sequenced and characterized the complete mitochondrial genome of the butterfly. The circular genome of 15,282?bp in length contains 13 protein-coding genes, 22 transfer RNA genes, 2 ribosomal RNA genes and a non-coding AT-rich region. Overall base composition of the complete mt genome was 33.97% A, 39.90% T, 12.38% C and 7.75% G, with an AT bias of 79.87%. All protein-coding genes start with an ATN codon, and terminate with the typical stop codon TAA or a single T. The non-coding AT-rich region is 331?bp in length, including a 18?bp poly-T stretch and a microsatellite-like (TA)7 element. The genome sequence is useful for future studies of phylogenetics, molecular evolution, conservation genetics and agricultural control.
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AFM lateral force calibration for an integrated probe using a calibration grating.
Ultramicroscopy
PUBLISHED: 10-09-2013
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Atomic force microscopy (AFM) friction measurements on hard and soft materials remain a challenge due to the difficulties associated with accurately calibrating the cantilever for lateral force measurement. One of the most widely accepted lateral force calibration methods is the wedge method. This method is often used in a simplified format but in so doing sacrifices accuracy. In the present work, we have further developed the wedge method to provide a lateral force calibration method for integrated AFM probes that is easy to use without compromising accuracy and reliability. Raw friction calibration data are collected from a single scan image by continuous ramping of the set point as the facets of a standard grating are scanned. These data are analysed to yield an accurate lateral force conversion/calibration factor that is not influenced by adhesion forces or load deviation. By demonstrating this new calibration method, we illustrate its reliability, speed and ease of execution. This method makes accessible reliable boundary lubrication studies on adhesive and heterogeneous surfaces that require spatial resolution of frictional forces.
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Oral bisphosphonates and the risk of colorectal cancer: a meta-analysis.
J. Clin. Gastroenterol.
PUBLISHED: 09-14-2013
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: To conduct a meta-analysis on the relationship between the usage of bisphosphonates and the risk of colorectal cancer.
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Desmoplastic fibroma of the spine: a series of 12 cases and outcomes.
Spine J
PUBLISHED: 08-28-2013
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Desmoplastic fibroma (DF) is a benign, yet locally aggressive, tumor of the connective tissue. Desmoplastic fibroma in the spine is extremely rare, and only a few cases have been reported. Although surgical resection of DF arising in the spine is commonly regarded as a recommended treatment, it is difficult to achieve satisfactory results.
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A novel mutation of DNAH5 in chronic rhinosinusitis and primary ciliary dyskinesia in a Chinese family.
Eur Arch Otorhinolaryngol
PUBLISHED: 08-12-2013
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The genetic factors underlying the pathogenesis of chronic rhinosinusitis (CRS) remains unclear. We herein identified four related subjects with CRS and primary ciliary dyskinesia (PCD) from geographically disperse Chinese Han communities and performed exome capture and sequencing of one affected individual and unaffected parents. We found a novel mutation in DNAH5 (c. 8030G>A) in CRS and PCD which was different from those attributed to cystic fibrosis and a defect of cilia motility in a Chinese family through exome capture and sequencing. Our findings showed that c. 8030G>A of DNAH5 may be implicated as the disease-causing gene of CRS and PCD in this Chinese family, which may expand the understanding of clinicians on the pathogenesis of CRS. Moreover, the identification of this novel mutation in DNAH5 indirectly indicates that exome capture and sequencing are beneficial in the genetic research of midget consanguinity families.
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Improved Polysaccharide Production in Submerged Culture of Ganoderma lucidum by the Addition of Coixenolide.
Appl. Biochem. Biotechnol.
PUBLISHED: 07-15-2013
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Polysaccharides from Ganoderma lucidum have various bioactivities and have been widely used as nutraceuticals and functional foods. Coixenolide was added into the media to enhance the production of mycelia biomass and polysaccharides in the submerged culture of G. lucidum in this work. The results showed that when a level of 0.2 % coixenolide was added at day 1, the biomass, exopolysaccharide, and intracellular polysaccharide reached 5.224, 0.222, and 0.399 g l(-1), respectively, which were 1.39-fold, 2.58-fold, and 2.24-fold compared to that of control. Analysis of the fermentation kinetics of G. lucidum suggested that glucose concentration in the coixenolide-added group decreased more quickly as compared to the control group from days 3 to 9 of the fermentation process, and the polysaccharides biosynthesis were promoted at the same culture period. However, the culture pH profile was not affected by the addition of coixenolide. Enzyme activities analysis indicated that coixenolide affected the synthesis level of phosphoglucose isomerase and ?-phosphoglucomutase.
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[Effect of TUBB3, TS and ERCC1 mRNA expression on chemoresponse and clinical outcome of advanced gastric cancer by multiplex branched-DNA liquid chip technology].
Zhong Nan Da Xue Xue Bao Yi Xue Ban
PUBLISHED: 07-06-2013
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To analyze the impact of ?-tubulin-III (TUBB3), thymidylate synthase (TS) and excision repair cross complementation group 1 (ERCC1) mRNA expression on chemoresponse and clinical outcome of patients with advanced gastric cancer treated with TXT/CDDP/FU (DCF) regimen chemotherapy.
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Aqueous solution synthesis of reduced graphene oxide-germanium nanoparticles and their electrical property testing.
Nanoscale Res Lett
PUBLISHED: 07-01-2013
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Aqueous solution synthesis of reduced graphene oxide-germanium nanoparticles (RGO-GeNPs) was developed using graphene oxide (GO) as stabilizer, which could be conducive to obtain better excellent electrical properties. The information about morphology and chemical composition of the nanomaterials were obtained by TEM, FTIR, EDS, and XRD measurements. Stable aqueous dispersibility of RGO-GeNPs was further improved by poly(sodium 4-styrenesulfonate) (PSS) to obtain amphiphilic polymer-coated RGO-GeNPs (PSS-RGO-GeNPs). A possible mechanism to interpret the formation of RGO-GeNPs was proposed. The as-synthesized RGO-GeNPs showed excellent battery performance when used as an anode material for Li ion batteries. The resulting nanocomposites exhibited high specific capacity and good cycling stability after 80 cycles. This study showed a facile strategy to synthetize graphene and Ge nanocomposites which can be a hopeful anode material with excellent electrical properties for lithium ion batteries.
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Salt-enhanced removal of 2-ethyl-1-hexanol from aqueous solutions by adsorption on activated carbon.
J Colloid Interface Sci
PUBLISHED: 06-29-2013
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2-Ethyl-1-hexanol has extensive industrial applications in solvent extraction, however, in view of its potential pollution to environment, the removal and recovery of 2-ethyl-1-hexanol is considered an essential step toward its sustainable use in the future. In this work, we report the removal of 2-ethyl-1-hexanol from aqueous solutions containing salts in high concentrations by adsorption on a coal-based activated carbon. Adsorption thermodynamics showed that the experimental isotherms were conformed well to the Langmuir equation. Also it was found that inorganic salts, i.e. MgCl2 and CaCl2 in high concentration significantly enhanced the adsorption capacity from 223 mg/g in the deionized water to 277 mg/g in a saline water. This phenomenon of adsorption enhancement could be ascribed to the salt-out effect. Kinetic analysis indicated that adsorption kinetics follows the pseudo-second-order equation and the adsorption rate constants increase with the salt concentration. The dynamic breakthrough volume and adsorbed amount of 2-ethyl-1-hexanol were significantly elevated when the salt is present in the water. The dynamic saturated adsorption amount increased from 218.3mg/g in the deionized water to 309.5mg/g in a salt lake brine. The Tomas model was well applied to predict the breakthrough curves and determine the characteristics parameters of the adsorption column.
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JoVE Visualize is a tool created to match the last 5 years of PubMed publications to methods in JoVE's video library.

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In developing our video relationships, we compare around 5 million PubMed articles to our library of over 4,500 methods videos. In some cases the language used in the PubMed abstracts makes matching that content to a JoVE video difficult. In other cases, there happens not to be any content in our video library that is relevant to the topic of a given abstract. In these cases, our algorithms are trying their best to display videos with relevant content, which can sometimes result in matched videos with only a slight relation.