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Find video protocols related to scientific articles indexed in Pubmed.
An H?O?-responsive nanocarrier for dual-release of platinum anticancer drugs and O?: controlled release and enhanced cytotoxicity against cisplatin resistant cancer cells.
Chem. Commun. (Camb.)
PUBLISHED: 07-15-2014
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Synergistic release of platinum anticancer drugs and O2 can be achieved in an H2O2-responsive nanocarrier incorporated with catalase. Such a system combines the advantages of chemotherapy and oxygen therapy and demonstrated improved therapeutic efficacy against cisplatin resistant cell lines which often appear to be in hypoxia.
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A turn-on fluorescent Fe(3+) sensor derived from an anthracene-bearing bisdiene macrocycle and its intracellular imaging application.
Chem. Commun. (Camb.)
PUBLISHED: 03-27-2014
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Integrating N(2)-hydroxyethyldiethylenetriamine with anthracene gives a [2+2] macrocycle fluorescent sensor. This sensor displays an instant/reversible turn-on response specific to Fe(3+), which allows facile visualization of the Fe(3+)/Fe(2+) transition and intracellular Fe(3+) imaging.
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A red fluorescent turn-on probe for hydrogen sulfide and its application in living cells.
Chem. Commun. (Camb.)
PUBLISHED: 07-18-2013
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A novel selective fluorescent chemosensor has been synthesized with a phenanthrene-fused dipyrromethene structure. Selective binding of Cu(2+) by results in a complex that displays high selectivity and sensitivity for H2S. The signal transduction occurs via reversible formation-separation of the complex and CuS. Its potential utility for biological applications was confirmed by fluorescence imaging of H2S in live cells.
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Thienopyrrole-expanded BODIPY as a potential NIR photosensitizer for photodynamic therapy.
Chem. Commun. (Camb.)
PUBLISHED: 03-27-2013
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The synthesis and characterization of a highly photostable bromo-substituted BODIPY dye (I) fused-ring-expanded with thienopyrrole moieties is reported. The results of MTT assays and flow cytometric analyses in living HeLa cells demonstrate that I has a high singlet oxygen quantum yield (?? = 0.63) and exhibits photocytotoxicity upon irradiation in the NIR region making it potentially suitable for use in PDT.
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A near-infrared reversible fluorescent probe for peroxynitrite and imaging of redox cycles in living cells.
Chem. Commun. (Camb.)
PUBLISHED: 07-22-2011
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BzSe-Cy is a small-molecule fluorescent probe containing Se, which can respond reversibly to changes in ONOO(-) or reduced ascorbate and exhibit high sensitivity and selectivity for ONOO(-).
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A nanoprobe for nonprotein thiols based on assembling of QDs and 4-amino-2,2,6,6-tetramethylpiperidine oxide.
Biosens Bioelectron
PUBLISHED: 03-07-2011
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A new fluorescent nanoprobe, 4-amino-2,2,6,6-tetramethylpiperidine oxide (AT)-functionalized CdTe quantum dots (QDs-AT), was synthesized, for selective detection of nonprotein thiols based on electron transfer (ET). In the presence of nonprotein thiols, the nitroxide radicals in QDs-AT were converted to hydroxylamines, resulting in the fluorescence recovery of the quenched QDs. The detection mechanism of the probe was investigated using Rh-Se-2 probe. The nanoprobe has high sensitivity toward glutathione (GSH) with a detection limit of 7.1 × 10?? M. The fluorescent imaging of living cells showed that QDs-AT could distinguish the concentration differences of GSH in HL-7702 and HepG2 cells.
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A highly selective, cell-permeable fluorescent nanoprobe for ratiometric detection and imaging of peroxynitrite in living cells.
Chemistry
PUBLISHED: 01-15-2011
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Peroxynitrite (ONOO(-)) is a highly reactive species implicated in the pathology of numerous diseases and there is currently great interest in developing fluorescent probes that can selectively detect ONOO(-) in living cells. Herein, a polymeric micelle-based and cell-penetrating peptide-coated fluorescent nanoprobe that incorporates ONOO(-) indicator dye and reference dye for the ratiometric detection and imaging of ONOO(-) has been developed. The nanoprobe effectively avoids the influences from enzymatic reaction and high-concentration ·OH and ClO(-). The improved ONOO(-) selectivity of the nanoprobe is achieved by a delicate complementarity of properties between the nanomatrix and the embedded molecular probe (BzSe-Cy). This nanoprobe also has other attractive properties, such as good water solubility, photostability, biocompatibility, and near-infrared excitation and emission. Fluorescence imaging experiments by confocal microscopy show that this nanoprobe is capable of visualizing ONOO(-) produced in living cells and it exhibits very low toxicity and good membrane permeability. We anticipate that this technique will be a potential tool for the precise pathological understanding and diagnosis of ONOO(-)-related human diseases.
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Ratiometric fluorescence chemodosimeters for fluoride anion based on pyrene excimer/monomer transformation.
Chem. Commun. (Camb.)
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Two pyrene dimers containing an -O-Si-Si-O- or -O-Si-O- linkage have been designed which exhibit ratiometric excimer/monomer emission upon fluoride anion induced Si-O bond cleavage. Incorporation of the probe into water soluble polymeric nanoparticles enhances its intracellular uptake and displays ratiometric fluorescent sensing for F(-) in living cells.
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Enhancing the Stokes shift of BODIPY dyes via through-bond energy transfer and its application for Fe(3+)-detection in live cell imaging.
Chem. Commun. (Camb.)
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Two energy transfer cassettes that exhibit a large pseudo Stokes shift (up to 400 nm) due to efficient through-bond energy transfer (up to 99%) have been constructed. Selective binding of Fe(III) with the donor entity significantly suppresses the excitation energy transfer resulting in fluorescence quenching in aqueous solution and in living cells.
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What is Visualize?

JoVE Visualize is a tool created to match the last 5 years of PubMed publications to methods in JoVE's video library.

How does it work?

We use abstracts found on PubMed and match them to JoVE videos to create a list of 10 to 30 related methods videos.

Video X seems to be unrelated to Abstract Y...

In developing our video relationships, we compare around 5 million PubMed articles to our library of over 4,500 methods videos. In some cases the language used in the PubMed abstracts makes matching that content to a JoVE video difficult. In other cases, there happens not to be any content in our video library that is relevant to the topic of a given abstract. In these cases, our algorithms are trying their best to display videos with relevant content, which can sometimes result in matched videos with only a slight relation.