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Rhizobium sophorae sp. nov. and Rhizobium sophoriradicis sp. nov., nitrogen-fixing rhizobial symbionts of medicinal legume Sophora flavescens in China.
Int. J. Syst. Evol. Microbiol.
PUBLISHED: 11-12-2014
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Five bacterial strains representing 45 isolates originated from root nodules of the medicinal legume Sophora flavescens were defined as two novel groups in the genus Rhizobium based on their phylogenetic relationships estimated from 16S rRNA genes and the housekeeping genes recA, glnII and atpD. These groups were distantly related to R. leguminosarum USDA 2370T (95.6% similarity for group I) and R. phaseoli ATCC 14482T (93.4% similarity for group II) in the multilocus sequence analysis (MLSA). In DNA-DNA hybridization, the reference strains CCBAU 03386T (group I) and CCBAU 03470T (group II) showed relatedness of 17.9%-57.8% and 11.0%-42.9%, respectively, with the type strains of related species. Both strains CCBAU 03386T and CCBAU 03470T contained Q-10 as major respiratory quinone and possessed 16:0, 18:0, 19:0 cyclo ?8c, summed feature 8, and summed feature 2 as major fatty acids, but did not contain 20:3 ?6,8,12c. Phenotypic features distinguishing both groups from all closely related Rhizobium species were found. Therefore, two novel species, Rhizobium sophorae sp. nov. for group I (type strain CCBAU 03386T = E5T = LMG 27901T = HAMBI 3615T), and Rhizobium sophoriradicis sp. nov., for group II (type strain CCBAU 03470T =C-5-1T = LMG 27898T = HAMBI 3510T), were proposed. Both groups could nodulate Phaseolus vulgaris and their hosts of origin (Sophora flavescens) effectively and their nodulation gene nodC was phylogenetically located in the symbiovar phaseoli.
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Phyllobacterium sophorae sp. nov., a novel symbiotic bacterium isolated from root nodules of Sophora flavescens.
Int. J. Syst. Evol. Microbiol.
PUBLISHED: 11-08-2014
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Two novel Gram-negative strains (CCBAU 03422T and CCBAU 03415) isolated from root nodules of Sophora flavescens were phylogenetically classified into the genus of Phyllobacterium based on the comparative analysis of 16S rRNA and atpD genes. They showed 99.8% rRNA gene sequence similarities to Phyllobacterium brassicacearum LMG 22836T, and CCBAU 03422T showed 91.2% and 88.6% atpD gene similarities to strains P. endophyticum LMG 26470T and P. brassicacearum LMG 22836T, respectively. Strain CCBAU 03422T contained Q-10 as its major quinone and showed cellular fatty acid profile, carbon source utilization and other phenotypic characteristics differing from type strains of the related species. DNA-DNA relatedness (lower than 48.8%) further confirmed the differences between the novel strains and the type strains of related species. Strain CCBAU 03422T could nodulate and fix nitrogen effectively on its original host plant, Sophora flavescens. Based upon the studies mentioned above, a novel species named as Phyllobacterium sophorae is proposed and the type strain is CCBAU 03422T (= A-6-3T = LMG 27899T = HAMBI 3508T).
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Applications of lasers in dental implantology.
Zhongguo Yi Xue Ke Xue Yuan Xue Bao
PUBLISHED: 11-01-2014
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With the constant progress of laser physics,medical laser technology has been widely applied in clinical practices and basic researches. In this article,we reviewed the relavent articles on the laser applications in dental implantology and concluded that lasers provides promising solutions in the treatment technology of dental implants and in the treatment of soft and hard tissue conditions.
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Two new phenol derivatives from Stereum hirsutum FP-91666.
J Asian Nat Prod Res
PUBLISHED: 10-09-2014
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Two new phenol derivatives, 2-(3-methyl-2-buten-1-yl)-4-methoxyethyl-phenol (1) and 5-hydroxy-4-(hydroxymethyl)-2-(3-methylbut-2-en-1-yl)cyclohex-4-en-1-one (2), together with eight known compounds consisting of phenol derivatives (3 and 4), niacinamide (5), and five ergosta type compounds (6-10), were isolated from solid fermentation products of Stereum hirsutum FP-91666. Two new structures were elucidated by extensive spectroscopic methods, including 1D NMR and 2D NMR, and HR-EI-MS experiments.
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[Studies on main interspecific association of rare and endangered medicinal plant Sinopodophyllum hexandrum community in Kangding Zheduo mountain of Sichuan province].
Zhongguo Zhong Yao Za Zhi
PUBLISHED: 10-04-2014
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Based on the 2 x 2 contingency table, by using multi-species relevance (variance ratio, VR), chi2-test, Ochiai index, Dice index, Jaccard index, t-test of v/x and F-test of Morisita, s index, the interspecific relationships and the spatial distribution pattern between 20 dominants in Kangding Zheduo Mountain of Sichuan province were studied. The results indicated that the interspecific association between dominants of Sinopodophyllum hexandrum community in this area did not show significant association, which suggested that the S. hexandrum community was in mature stage, and showed stronger independency, among total 190 pairs in 20 dominant species, 2 species pairs exhibited extremely significantly positive association, 12 species pairs showed significantly positive association, 6 species pairs exhibited significantly negative association and there were no pairs showed extremely significantly negative association. S. hexandrum in community did not show significant association, which indicates they are independent in community, the spatial distribution pattern of S. hexandrum is characterized by random distribution.
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An in vitro and in vivo comparison of solid and liquid-oil cores in transdermal aconitine nanocarriers.
J Pharm Sci
PUBLISHED: 09-03-2014
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This study compared transdermal aconitine delivery using solid lipid nanoparticles (SLN) and microemulsion (ME) vehicles. Aconitine-loaded SLN and ME were formulated with the same surfactant, cosurfactant, and water content, with an equal amount of oil matrix (ATO 888 for SLN and ethyl oleate for ME). These nanosized formulations (70-90 nm) showed suitable pH values and satisfactory skin tissue biocompatibility. SLN contained a higher concentration of smaller nanoparticles, compared with that in ME. Neither of the nanocarriers penetrated across excised skin in their intact form. In vitro transdermal delivery studies found that transdermal aconitine flux was lower from SLN than from ME (p < 0.05), but skin aconitine deposition was higher using SLN (p < 0.05). Fluorescence-activated cell sorting indicated that in vitro uptake of fluorescently labeled SLN by human immortalized keratinocyte (HaCaT) cells was greater than that of ME, indicating that a transcellular pathway may contribute to cutaneous drug absorption more effectively from SLN. In vivo studies found that these formulations could loosen stratum corneum layers and increase skin surface crannies, which may also enhance transdermal aconitine delivery. SLN produced a more sustained aconitine release, indicating that compared with ME, this transdermal delivery vehicle may reduce the toxicity of this drug. © 2014 Wiley Periodicals, Inc. and the American Pharmacists Association J Pharm Sci 103:3602-3610, 2014.
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Maximal entropy random walk for region-based visual saliency.
IEEE Trans Cybern
PUBLISHED: 08-20-2014
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Visual saliency is attracting more and more research attention since it is beneficial to many computer vision applications. In this paper, we propose a novel bottom-up saliency model for detecting salient objects in natural images. First, inspired by the recent advance in the realm of statistical thermodynamics, we adopt a novel mathematical model, namely, the maximal entropy random walk (MERW) to measure saliency. We analyze the rationality and superiority of MERW for modeling visual saliency. Then, based on the MERW model, we establish a generic framework for saliency detection. Different from the vast majority of existing saliency models, our method is built on a purely region-based strategy, which is able to yield high-resolution saliency maps with well preserved object shapes and uniformly highlighted salient regions. In the proposed framework, the input image is first over-segmented into superpixels, which are taken as the primary units for subsequent procedures, and regional features are extracted. Then, saliency is measured according to two principles, i.e., uniqueness and visual organization, both implemented in a unified approach, i.e., the MERW model based on graph representation. Intensive experimental results on publicly available datasets demonstrate that our method outperforms the state-of-the-art saliency models.
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[Study on structural properties of biochar under different materials and carbonized by FTIR].
Guang Pu Xue Yu Guang Pu Fen Xi
PUBLISHED: 07-11-2014
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Infrared spectroscopy (IR) is an important means of seeing the characteristics of the structural properties. Fourier transform infrared spectroscopy (FTIR) was applied to analyze the structural properties of biochar from different materials with different methods. The results showed that: the biochars have IR absorption peaks of hydroxyls group, aromatic group and containing organic group with the activated? charcoal, but in other absorption peaks, with a significant difference. The high temperature can make -OH, -CH3, -CH2-, -C=O to be associated or loss, and promotes the formation of aromatic groups during Carbonization of corn straw. At the different carbonization mode, the heating and microwave carbonization, has a carbonize mechanism of biochar, heating method may make -OH in alcohol and phenol to combinative with each other or loss, and to form benzene ring group and an aromatic group, Aromatic group in microwave method was so preventing to participate in the hot reaction, to form the more benzene substances. These results show that the Infrared spectrum can well analysis the structural characteristics of biochar, and showed that it comprises -OH, the aromatic group and other active groups.
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MicroRNA-378 inhibits cell growth and enhances l-OHP-induced apoptosis in human colorectal cancer.
IUBMB Life
PUBLISHED: 06-22-2014
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MicroRNAs (miRNAs) are small noncoding RNAs that participate in a variety of biological processes, and dysregulation of miRNAs is widely associated with cancer development and progression. MiR-378 is frequently downregulated in colorectal cancer (CRC) and colorectal cell lines; however, it has high serum levels. Bioinformatics analysis further deduced that CDC40 is a potential target of miR-378, and luciferase reporter assays confirmed the direct regulation of CDC40 by miR-378. CDC40 plays a key role in cell cycle progression through G1/S and G2/M and pre-mRNA splicing. Subsequently, we determined that miR-378 inhibits cell growth and the G1/S transition in CRC cells and that these effects were CDC40-dependent. Finally, miR-378 increased cell apoptosis induced by the chemotherapeutic drug l-OHP. Our data highlight the potential application of miR-378 as a tumor suppressor for CRC therapy and overcoming chemoresistance, and it may also be a potential tumor marker for CRC prognosis. © 2014 IUBMB Life, 66(9):645-654, 2014.
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Abundance and diversity of soybean-nodulating rhizobia in black soil are impacted by land use and crop management.
Appl. Environ. Microbiol.
PUBLISHED: 06-20-2014
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To investigate the effects of land use and crop management on soybean rhizobial communities, 280 nodule isolates were trapped from 7 fields with different land use and culture histories. Besides the known Bradyrhizobium japonicum, three novel genospecies were isolated from these fields. Grassland (GL) maintained a higher diversity of soybean bradyrhizobia than the other cultivation systems. Two genospecies (Bradyrhizobium spp. I and III) were distributed widely in all treatments, while Bradyrhizobium sp. II was found only in GL treatment. Cultivation with soybeans increased the rhizobial abundance and diversity, except for the soybean monoculture (S-S) treatment. In monoculture systems, soybeans favored Bradyrhizobium sp. I, while maize and wheat favored Bradyrhizobium sp. III. Fertilization decreased the rhizobial diversity indexes but did not change the species composition. The organic carbon (OC) and available phosphorus (AP) contents and pH were the main soil parameters positively correlated with the distribution of Bradyrhizobium spp. I and II and Bradyrhizobium japonicum and negatively correlated with Bradyrhizobium sp. III. These results revealed that different land uses and crop management could not only alter the diversity and abundance of soybean rhizobia, but also change interactions between rhizobia and legume or nonlegume plants, which offered novel information about the biogeography of rhizobia.
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Monocyte subsets and monocyte-platelet aggregates in patients with unstable angina.
J. Thromb. Thrombolysis
PUBLISHED: 05-22-2014
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Monocyte subsets and monocyte-platelet aggregates (MPAs) play important role in atherosclerosis and thrombosis. We aimed to determine their changes in patients with unstable angina (UA). In this cross-sectional case-control study, Global Registry of Acute Coronary Events (GRACE) score was determined in 95 UA patients without elevated troponin level. Thirty age-and-sex matched stable coronary heart disease (CHD) subjects served as control group. The classical (CD14++CD16-, Mon1), the intermediate (CD14++CD16+, Mon2) and the non-classical (CD14+CD16++, Mon3) monocytes, as well as subset-specific MPAs, were measured by flow cytometry. Compared with stable CHD patients, UA patients had increased Mon2 and Mon3 counts (all P < 0.001). For UA subjects, compared with GRACE score-determined low risk patients (GRACE score ?108, n = 70), intermediate-to-high risk patients (GRACE score >108, n = 25) had higher counts of Mon2 and total MPAs, as well as Mon1- and Mon2-associated MPAs (all P < 0.001). Adjusted binary logistic regression analysis revealed that increased counts of Mon2 subset (for per 5 cells/?L increase, OR 1.186, 95% CI 1.044-1.347, P = 0.009), Mon2 MPAs (for per 5 cells/?L increase, OR 1.228, 95% CI 1.062-1.421, P = 0.006) and total MPAs (for per 5 cells/?L increase, OR 1.072, 95 % CI 1.010-1.137, P = 0.022) independently associated with GRACE score-determined intermediate-to-high risk UA patients. In UA patients with intermediate-to-high risk (determined by GRACE score), counts of Mon2 subset, Mon2-associated MPAs and total MPAs are increased, which are independent of traditional risk factors.
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Factorial study of moxibustion in treatment of diarrhea-predominant irritable bowel syndrome.
World J. Gastroenterol.
PUBLISHED: 05-16-2014
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To identify an appropriate therapeutic regimen for using aconite cake-separated moxibustion to treat diarrhea-predominant irritable bowel syndrome (D-IBS).
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Evaluation of skin viability effect on ethosome and liposome-mediated psoralen delivery via cell uptake.
J Pharm Sci
PUBLISHED: 04-30-2014
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This study investigated the effect of skin viability on its permeability to psoralen delivered by ethosomes, as compared with liposomes. With decreasing skin viability, the amount of liposome-delivered psoralen that penetrated through the skin increased, whereas skin deposition of psoralen from both ethosomes and liposomes reduced. Psoralen delivery to human-immortalized epidermal cells was more effective using liposomes, whereas delivery to human embryonic skin fibroblast cells was more effective when ethosomes were used. These findings agreed with those of in vivo studies showing that skin psoralen deposition from ethosomes and liposomes first increased and then plateaued overtime, which may indicate gradual saturation of intracellular drug delivery. It also suggested that the reduced deposition of ethosome- or liposome-delivered psoralen in skin with reduced viability may relate to reduced cellular uptake. This work indicated that the effects of skin viability should be taken into account when evaluating nanocarrier-mediated drug skin permeation.
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Risk of cerebral arteriovenous malformation rupture during pregnancy and puerperium.
Neurology
PUBLISHED: 04-23-2014
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To determine whether the risk of arteriovenous malformation (AVM) rupture is increased during pregnancy and puerperium.
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Preventive effect of hydrazinocurcumin on carcinogenesis of diethylnitrosamine-induced hepatocarcinoma in male SD rats.
Asian Pac. J. Cancer Prev.
PUBLISHED: 04-11-2014
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The purpose of the present study was to evaluate the preventive effects of hydrazinocurcumin (HZC) on diethylnitrosamine (DEN)-induced hepatocarcinogenesis in a male Sprague Dawley (SD) rat model. One hundred and twenty male SD rats used in this study were divided into six groups. Those receiving DEN with curcumin (CUR) or HZC were studied compared with the DEN-alone group. The study demonstrated that DEN induced severe histological and immunohistochemical changes in liver tissues, significantly increasing the levels of liver marker enzymes (alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), ?-glutamyltransferase (GGT) and total bilirubin level (TBL)). The hepatocarcinoma incidences were 100.0%, 36.7% and 20.0% in the DEN-alone, DEN-CUR and DEN-HZC groups, respectively. Although macroscopic and microscopic features suggested that both CUR and HZC were effective in inhibiting DEN- induced hepatocarcinogenesis, HZC was exerted a stronger influence. Immunohistochemical analysis with PCNA demonstrated significantly differences among the groups (all P < 0.05). Taken together, the results suggested application of CUR and HZC could prevent the occurrence of carcinogenesis and HZC may be a more potent compound for prevention of DEN-induced hepatocarcinogenesis in rats than CUR.
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Comparison of ethosomes and liposomes for skin delivery of psoralen for psoriasis therapy.
Int J Pharm
PUBLISHED: 04-05-2014
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Recent reports have indicated that psoriasis may be caused by malfunctioning dermal immune cells, and psoralen ultraviolet A (PUVA) is an effective treatment for this chronic disease. However, conventional topical formulations achieve poor drug delivery across patches of psoriasis to their target sites. The present study describes the development of a novel psoralen transdermal delivery system employing ethosomes, flexible vesicles that can penetrate the stratum corneum and target deep skin layers. An in vitro skin permeation study showed that the permeability of psoralen-loaded ethosomes was superior to that of liposomes. Using ethosomes, psoralen transdermal flux and skin deposition were 38.89±0.32 ?g/cm(2)/h and 3.87±1.74 ?g/cm(2), respectively, 3.50 and 2.15 times those achieved using liposomes, respectively. The ethosomes and liposomes were found to be safe following daily application to rat skin in vivo, for 7 days. The ethosomes showed better biocompatibility with human embryonic skin fibroblasts than did an equivalent ethanol solution, indicating that the phosphatidylcholine present in ethosome vesicles improved their biocompatibility. These findings indicated that ethosomes could potentially improve the dermal and transdermal delivery of psoralen and possibly of other drugs requiring deep skin delivery.
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Desolvation and dehydrogenation of solvated magnesium salts of dodecahydrododecaborate: relationship between structure and thermal decomposition.
Chemistry
PUBLISHED: 02-20-2014
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Attempts to synthesize solvent-free MgB12H12 by heating various solvated forms (H2O, NH3, and CH3OH) of the salt failed because of the competition between desolvation and dehydrogenation. This competition has been studied by thermogravimetric analysis (TGA) and temperature-programmed desorption (TPD). Products were characterized by IR, solution- and solid-state NMR spectroscopy, elemental analysis, and single-crystal or powder X-ray diffraction analysis. For hydrated salts, thermal decomposition proceeded in three stages, loss of water to form first hexahydrated then trihydrated, and finally loss of water and hydrogen to form polyhydroxylated complexes. For partially ammoniated salts, two stages of thermal decomposition were observed as ammonia and hydrogen were released with weight loss first of 14?% and then 5.5?%. Thermal decomposition of methanolated salts proceeded through a single step with a total weight loss of 32?% with the release of methanol, methane, and hydrogen. All the gaseous products of thermal decomposition were characterized by using mass spectrometry. Residual solid materials were characterized by solid-state (11)B magic-angle spinning (MAS)?NMR spectroscopy and X-ray powder diffraction analysis by which the molecular structures of hexahydrated and trihydrated complexes were solved. Both hydrogen and dihydrogen bonds were observed in structures of [Mg(H2O)6B12H12]?6?H2O and [Mg(CH3OH)6B12H12]?6?CH3OH, which were determined by single-crystal X-ray diffraction analysis. The structural factors influencing thermal decomposition behavior are identified and discussed. The dependence of dehydrogenation on the formation of dihydrogen bonds may be an important consideration in the design of solid-state hydrogen storage materials.
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Increase of microbial growth potential in municipal secondary effluent by coagulation.
Chemosphere
PUBLISHED: 02-15-2014
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Microbial growth is a big issue of concern in the use of reclaimed water. In this study, the variation of microbial growth potentials of municipal secondary effluents after coagulation was evaluated by measuring assimilable organic carbon (AOC). Surprisingly, the AOC levels increased significantly (55-667%) after coagulation with poly-aluminum dosages of 60 mgL(-1) for the samples investigated in this research. By ultrafiltration membrane fractionation, the microbial growth potentials of the fractions with different molecular weight (MW) were measured. The results revealed that the maximum cell densities of microbial growth in secondary effluents were lower than those in their fractions with MW<10kDa. Meanwhile, the organic component with MW>10kDa in biological treated effluents was proved to have an inhibitory effect on microbial growth. Therefore, the removal of those high MW organic matters was the main reason for the increase of microbial growth potential in secondary effluents during coagulation. Furthermore, polysaccharides and/or proteins in secondary effluents were easily removed by coagulation and were thought to be the possible key organic substances affecting the microbial growth potential during coagulation. It is suggested that post treatments would be needed after coagulation to maintain the biological stability of reclaimed water.
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Randomized controlled trial: moxibustion and acupuncture for the treatment of Crohn's disease.
World J. Gastroenterol.
PUBLISHED: 02-10-2014
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To evaluate the clinical efficacy and safety of acupuncture and moxibustion for the treatment of active Crohn's disease (CD).
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Management of giant posttraumatic abdominal aortic pseudoaneurysm and aortic occlusion using a unique hybrid procedure combining transcatheter device closure and open surgical repair.
Ann Vasc Surg
PUBLISHED: 02-05-2014
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Posttraumatic abdominal aortic pseudoaneurysm (AAP) is a rare but life-threating disease. Here, we described a case of posttraumatic AAP with acute abdominal aortic occlusion and its successful treatment using a unique hybrid procedure. An 18-year-old male was referred, with a giant AAP among visceral arteries, which occluded the aorta and left renal artery. An infrequently used ventricular septal device was delivered via femoral access and successfully plugged the tear. Then the isolated pseudoaneurysm was resected through open surgery without major bleeding. At 6 months after operation, the patient was alive without evidence of complications. This hybrid procedure combining transcatheter device closure and open surgery was a successful attempt in the subemergency treatment of posttraumatic AAP.
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Intracranial hemorrhage from moyamoya disease during pregnancy and puerperium.
Int J Gynaecol Obstet
PUBLISHED: 01-22-2014
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To determine the incidence of hemorrhage among women with moyamoya disease (MMD) during pregnancy and puerperium.
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Effect of low-dose tacrolimus with mycophenolate mofetil on renal function following liver transplantation.
World J. Gastroenterol.
PUBLISHED: 01-12-2014
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To determine whether low-dose tacrolimus (TAC) combined with mycophenolate mofetil (MMF) is a safe approach to decrease the incidence of chronic kidney disease (CKD) in liver transplantation (LT) recipients.
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Risk factors associated with early and late HAT after adult liver transplantation.
World J. Gastroenterol.
PUBLISHED: 01-01-2014
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To identify risk factors that might contribute to hepatic artery thrombosis (HAT) after liver transplantation (LT).
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Evaluation of psoralen ethosomes for topical delivery in rats by using in vivo microdialysis.
Int J Nanomedicine
PUBLISHED: 01-01-2014
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This study aimed to improve skin permeation and deposition of psoralen by using ethosomes and to investigate real-time drug release in the deep skin in rats. We used a uniform design method to evaluate the effects of different ethosome formulations on entrapment efficiency and drug skin deposition. Using in vitro and in vivo methods, we investigated skin penetration and release from psoralen-loaded ethosomes in comparison with an ethanol tincture. In in vitro studies, the use of ethosomes was associated with a 6.56-fold greater skin deposition of psoralen than that achieved with the use of the tincture. In vivo skin microdialysis showed that the peak concentration and area under the curve of psoralen from ethosomes were approximately 3.37 and 2.34 times higher, respectively, than those of psoralen from the tincture. Moreover, it revealed that the percutaneous permeability of ethosomes was greater when applied to the abdomen than when applied to the chest or scapulas. Enhanced permeation and skin deposition of psoralen delivered by ethosomes may help reduce toxicity and improve the efficacy of long-term psoralen treatment.
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[Cumulative analgesic effects of EA stimulation of sanyinjiao (SP 6) in primary dysmenorrhea patients: a multicenter randomized controlled clinical trial].
Zhen Ci Yan Jiu
PUBLISHED: 12-07-2013
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To compare the cumulative analgesic effect of electroacupuncture (EA) stimulation of Sanyinjiao (SP 6), Xuanzhong (GB 39) and non-acupoint for primary dysmenorrhea patients.
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Clinical values of intraoperative indocyanine green fluorescence video angiography with Flow 800 software in cerebrovascular surgery.
Chin. Med. J.
PUBLISHED: 11-19-2013
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Microscope-integrated near-infrared indocyanine green video angiography (ICG-VA) has been used in neurosurgery for a decade. This study aimed to assess the value of intraoperative indocyanine green (ICG) video angiography with Flow 800 software in cerebrovascular surgery and to discover its hemodynamic features and changes of cerebrovascular diseases during surgery.
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First-principles prediction of magnetic superatoms in 4d-transition-metal-doped magnesium clusters.
J Chem Phys
PUBLISHED: 11-12-2013
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We theoretically predict magnetic superatoms in the 4d-transition-metal-doped Mg8 clusters using a spin-polarized density functional theory method. We demonstrate that TcMg8 is highly energetically stable in both structure and magnetic states, and identify it as a magnetic superatom with a magnetic moment as large as 5 ?B. The magnetic TcMg8 with 23 valence electrons has a configuration of 1S(2)1P(6)1D(10) closed shell and 2S(1)2D(4) open shell, complying with Hunds rule similar to the single atom. We elucidate the formation mechanism of the magnetic TcMg8 superatom based on the detailed analysis of molecular orbitals, and attribute it to the large exchange interaction and moderate crystal field effect. Finally, we predict that the magnetic TcMg8 may exhibit semiconductor-like property with spin polarization characteristics.
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[Surgical treatment of ruptured cerebellar arteriovenous malformations].
Zhonghua Yi Xue Za Zhi
PUBLISHED: 10-16-2013
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To analyze the clinical characteristics and surgical outcomes of ruptured cerebellar arteriovenous malformations (AVM).
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Disease location identification as a major guide to acupoint selection.
Chin J Integr Med
PUBLISHED: 09-26-2013
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Selecting acupoints according to channel pathway is the major point selection principle in acupuncture treatment for which there is a consensus. However, defining the disease location remains the premise of acupoint selection based on channel pathway. What is the substance of disease location identification in clinical acupuncture? Besides indicating acupoint selection according to channel pathway, what guidance can it provide for acupuncture treatment? This is the main topic discussed in this article.
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Preparation and in vitro anti-tumor properties of toad venom extract-loaded solid lipid nanoparticles.
Pharmazie
PUBLISHED: 09-12-2013
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In this study, we prepared solid lipid nanoparticles (TV-SLNs) loaded with toad venom extract and investigated their anti-tumor effects in vitro in HeLa and SKOV-3 cells. TV-SLNs were prepared using a cold homogenization technique, and the formulation was optimized by central composite design and response surface methods. The anti-tumor activities of TV-SLNs were evaluated by analyzing cell division and cell cycle distribution by using the MTT assay and flow cytometry. After incubation with TV-SLNs, the growth of both HeLa and SKOV-3 cells was inhibited significantly. The percentage of HeLa cells in G0/G1 phase decreased, whereas that in the S and G2/M phases increased. Thus, the S and G2/M phases were blocked after the incubation of HeLa cells with TV-SLNs for 24 h. In contrast, the percentage of SKOV-3 cells in G0/G1 phase increased and then decreased in S and G2/M phases, with the G0/G1 phase being blocked after incubation with TV-SLNs for 24 h. Our results demonstrate that TV-SLNs inhibited the fissiparism of HeLa and SKOV-3 cells in a time-and dose-dependent manner. TV-SLNs may be effective as a novel TV vaginal delivery system for the treatment of cervical and ovarian cancers.
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The roles of dihydrogen bonds in amine borane chemistry.
Acc. Chem. Res.
PUBLISHED: 09-10-2013
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A dihydrogen bond (DHB) is an electrostatic interaction between a protonic hydrogen and a hydridic hydrogen. Over the past two decades, researchers have made significant progress in the identification and characterization of DHBs and their properties. In comparison with conventional hydrogen bonds (HBs), which have been widely used in catalysis, molecular recognition, crystal engineering, and supramolecular synthesis, chemists have only applied DHBs in very limited ways. Considering that DHBs and conventional HBs have comparable strength, DHBs could be more widely applied in chemistry. Over the past several years, we have explored the impact of DHBs on amine borane chemistry and the syntheses and characterization of amine boranes and ammoniated metal borohydrides for hydrogen storage. Through systematic computational and experimental investigations, we found that DHBs play a dominant role in dictating the reaction pathways (and thus different products) of amine boranes where oppositely charged hydrogens coexist for DHB formation. Through careful experiments, we observed, for the first time, a long-postulated reaction intermediate, ammonia diborane (AaDB), whose behavior is essential to mechanistic understanding of the formation of the diammoniate of diborane (DADB) in the reaction of ammonia (NH3) with tetrahydrofuran borane (THF·BH3). The formation of DADB has puzzled the boron chemistry community for decades. Mechanistic insight enabled us to develop facile syntheses of aminodiborane (ADB), ammonia borane (AB), DADB, and an inorganic butane analog NH3BH2NH2BH3 (DDAB). Our examples, together with those in the literature, reinforce the fact that DHB formation and subsequent molecular hydrogen elimination are a viable approach for creating new covalent bonds and synthesizing new materials. We also review the strong effects of DHBs on the stability of conformers and the hydrogen desorption temperatures of boron-nitrogen compounds. We hope that this Account will encourage further applications of DHBs in molecular recognition, host-guest chemistry, crystal engineering, supramolecular chemistry, molecular self-assembly, chemical kinetics, and the syntheses of new advanced materials.
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[QSAR/QSPR for predicting the toxicity of imidazolium ionic liquids].
Huan Jing Ke Xue
PUBLISHED: 08-07-2013
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Ionic liquids have received lots of attention due to their physical and chemical characteristics. They are honoured the sustainable "Green Solvent". In this paper, the QSPR/QSAR (quantitative structure-property/activity relationships) method was used to study the quantitative relationship between the toxicity and structure of 43 kinds of imidazolium ionic liquids, 10 kinds of substances were used to carry out the external test. The model contains six structural descriptors selected from heuristic method, and R2, R2(CV), F and S2 of the model were 0.921, 0.894, 70.35, 0.098 respectively. Test set was used to conduct external validation, and the R2 was 0.952. The result showed that this model had good reliability, and can be used to predict the toxicity of imidazolium ionic liquids.
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Cancer pain management at home: voice from an underdeveloped region of China.
Cancer Nurs
PUBLISHED: 08-06-2013
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Pain is a major problem for patients with advanced cancer and one of the most frequent and disturbing of all cancer-related symptoms. Researchers continue to report that cancer pain remains undertreated. Inadequate pain control can significantly affect the patients quality of life and may in turn affect the patients will to live or comply with treatment recommendations. A better understanding of the experience of cancer pain management is important in identifying factors responsible for undertreated pain.
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Invited article: micron resolution spatially resolved measurement of heat capacity using dual-frequency time-domain thermoreflectance.
Rev Sci Instrum
PUBLISHED: 08-02-2013
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A pump-probe photothermal technique - dual-frequency time-domain thermoreflectance - was developed for measuring heat capacity with a spatial resolution on the order of 10 ?m. The method was validated by measuring several common materials with known heat capacity. Rapid measurement of composition-phase-property relationships was demonstrated on Ti-TiSi2 and Ni-Zr diffusion couples; experimental values of heat capacity of the intermetallic compounds in these diffusion couples were compared with literature values and CALPHAD (CALculation of PHAse Diagram) calculations. The combination of this method and diffusion multiples provides an efficient way to generate thermodynamic data for CALPHAD modeling and database construction. The limitation of this method in measuring low thermal diffusivity materials is also discussed.
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[Preoperative location of major language functional cerebral areas using functional magnetic resonance imaging].
Zhonghua Yi Xue Za Zhi
PUBLISHED: 07-18-2013
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To design a set of functional magnetic resonance imaging (fMRI) tasks to activate the major Chinese language functional areas.
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Application of a peripheral nerve block technique in laser treatment of the entire facial skin and evaluation of its analgesic effect.
Eur J Dermatol
PUBLISHED: 06-26-2013
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Background: The aim was to develop a technique for peripheral nerve block anesthesia (PNB) for the skin of the entire face and neck, considering the nerves anesthetized, injection sites, use of an injection method assisted by computer-controlled local anesthetic delivery (CCLAD), and to evaluate the analgesic effects of the PNB technique. Methods: 50 patients who suffered from large nevus of Ota lesions or port-wine stains on their facial and neck skin and who required laser treatment were included. This study was designed as a non-randomized self-control trial. All the patients received the laser treatment under topical anesthesia in the first phase and three to six months later, they received the same treatment under the PNB using CCLAD. The differences in scores from the visual analogue scale of pain for the two phases were analyzed by the t-test. P values <0.05 were considered to be statistically significant. Results: The peripheral nerve block technique was simple to execute and easy to learn, the anesthetic injection site was generally located at a subcutaneous depth of 0.5-1.0 cm. The analgesic effect of PNB was significant, the mean pain score (2.8 ± 2.2) was significantly lower than that with topical anesthesia (P<0.0001). Patients during the PNB phase did not experience injection pain following CCLAD. Conclusion: The peripheral nerve block technique can greatly ease the pain that occurs during laser treatment, especially for patients with larger lesions. CCLAD will allow PNB to be broadly applied in laser treatments.
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The incidence of cerebral amyloid angiopathy in surgically treated intracranial hemorrhage in the Chinese population.
Neurosurg Rev
PUBLISHED: 06-14-2013
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Despite being widely accepted as an important cause of spontaneous intracranial hemorrhage (ICH), cerebral amyloid angiopathy (CAA) has seldom been studied in the Chinese population. The current study aims to investigate the incidence and features of CAA in surgically treated ICH patients in China. From May 2006 to April 2011, 974 patients admitted to 71 hospitals throughout China for acute spontaneous ICH were studied. Craniotomy for hematoma evacuation was performed. Brain tissue from the superficial side of the suspected residual hematoma cavity, as well as from the cortex and subcortex, was obtained. Congo Red stain and ?-amyloid immunohistochemistry were used for the diagnosis. Each case was assigned a pathological severity score. Of the 974 involved patients, 37.7% were identified with CAA of different degrees. CAA had positive correlation with age and was independent of sex. Most patients had mild CAA with only the superficial vessels involved in lobes instead of the basal ganglia; the patients ?65 years had more severe pathological score of CAA than those <65 years and had more lobes and cerebellum involved than the latter. More than one third of the surgically treated Chinese ICH patients may have CAA of different degrees.
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[Microscopic identification of tibetan medicinal herb "Songdi" (Saxifraga umbellulata var pectinata)].
Zhongguo Zhong Yao Za Zhi
PUBLISHED: 05-31-2013
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To establish a method of microscopic identification of Tibetan medicinal herb " Songdi" (Saxifraga umbellulata var. pectinata).
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[Genetic diversity and genetic structure of endangered wild Sinopodophyllum emodi by start codon targeted polymorphism].
Zhongguo Zhong Yao Za Zhi
PUBLISHED: 05-16-2013
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Revealed the genetic diversity level and genetic structure characteristics in Sinopodophyllum emodi, a rare and endangered species in China.
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Dynamic surface acoustic response to a thermal expansion source on an anisotropic half space.
J. Acoust. Soc. Am.
PUBLISHED: 05-10-2013
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The surface displacement response to a distributed thermal expansion source is solved using the reciprocity principle. By convolving the strain Greens function with the thermal stress field created by an ultrafast laser illumination, the complete surface displacement on an anisotropic half space induced by laser absorption is calculated in the time domain. This solution applies to the near field surface displacement due to pulse laser absorption. The solution is validated by performing ultrafast laser pump-probe measurements and showing very good agreement between the measured time-dependent probe beam deflection and the computed surface displacement.
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Thermolysis and solid state NMR studies of NaB3H8, NH3B3H7, and NH4B3H8.
Dalton Trans
PUBLISHED: 04-20-2013
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In an effort to broaden the search for high-capacity hydrogen storage materials, three triborane compounds, NaB(3)H(8), NH(3)B(3)H(7), and NH(4)B(3)H(8), were studied. In addition to hydrogen, thermal decomposition also releases volatile boranes, and the relative amounts and species depend on the cations (Na(+), NH(4)(+)) and the Lewis base (NH(3)). Static-sample hydrogen NMR is used to probe molecular motion in the three solids. In each case, the line width decreases from low temperatures to room temperature in accordance with a model of isotropic or nearly isotropic reorientations. Such motions also explain a deep minimum in the relaxation time T(1). Translational diffusion never appears to be rapid on the 10(-5) s time scale of NMR.
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Oxidation of bisphenol A by permanganate: reaction kinetics and removal of estrogenic activity.
Water Sci. Technol.
PUBLISHED: 04-13-2013
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The kinetics for reaction between bisphenol A (BPA) and permanganate was examined over pH range of 5.0-9.9 and the estrogenic activity of aqueous BPA solution after oxidation was assessed by yeast two-hybrid assay. Reaction kinetics follows the second-order rate law, with the apparent second-order rate constant of 15.1 ± 1.1 M(-1)s(-1) at pH 6.0 and 25°C and the activation energy of 48.7 kJ/mol. The kinetics exhibits pH dependency and the specific rate constants related to speciation of BPA are 50 ± 28 M(-1)s(-1), 9.6 (±0.6) × 10(3) M(-1)s(-1) and 1.4 (±0.1) × 10(4) M(-1)s(-1) for BPA, BPA(-) and BPA(2-), respectively. The results of the estrogenic/antiestrogenic activity test show that there is a hysteresis for the removal of estrogenic activity of aqueous BPA solution at pH 7.3. Removal of BPA is completed in 10 min, but complete removal of estrogenic activity requires a further 20 min.
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[Melanocytic neoplasms of central nervous system analysis].
Zhonghua Yi Xue Za Zhi
PUBLISHED: 04-13-2013
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To explore the clinical manifestations, diagnosis, treatment and prognostic features of melanocytic neoplasms of central nervous system (CNS).
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Facile synthesis of freestanding Si nanowire arrays by one-step template-free electro-deoxidation of SiO2 in a molten salt.
Chem. Commun. (Camb.)
PUBLISHED: 04-10-2013
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This communication presents a novel kind of silicon nanomaterial: freestanding Si nanowire arrays (Si NWAs), which are synthesized facilely by one-step template-free electro-deoxidation of SiO2 in molten CaCl2. The self-assembling growth process of this material is also investigated preliminarily.
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A clinical trial of acupuncture about time-varying treatment and points selection in primary dysmenorrhea.
J Ethnopharmacol
PUBLISHED: 03-13-2013
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To observe the clinical therapeutic effects of acupuncture at single point Shiqizhui (EX-B8) and multi-points in time-varying treatment for primary dysmenorrhea.
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Severe pathological manifestation of cerebral amyloid angiopathy correlates with poor outcome from cerebral amyloid angiopathy related intracranial hemorrhage.
Chin. Med. J.
PUBLISHED: 02-21-2013
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Cerebral amyloid angiopathy (CAA) is one of the main causes of spontaneous intracranial hemorrhage (ICH). No established link is available between pathological scores of CAA and its outcome. This study aimed to identify the correlations between pathological severity and poor postoperative outcome in the Chinese population.
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The pre-Kasai procedure in living donor liver transplantation for children with biliary atresia.
HBPD INT
PUBLISHED: 02-09-2013
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Biliary atresia (BA) is a major cause of chronic cholestasis, a fatal disorder in infants. This study was undertaken to evaluate the safety and effectiveness of primary living donor liver transplantation (LDLT) in comparison with the traditional first-line treatment, the Kasai procedure.
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In vivo microdialysis for the evaluation of transfersomes as a novel transdermal delivery vehicle for cinnamic acid.
Drug Dev Ind Pharm
PUBLISHED: 01-25-2013
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Abstract In this study, cinnamic acid-loaded transfersomes were prepared and dermal microdialysis sampling was used in Sprague-Dawley rats to compare the amount of drug released into the skin using transfersomes as transdermal carriers with that released on using conventional liposomes. The formulation of cinnamic acid-loaded transfersomes was optimized by a uniform design through in vitro transdermal permeation studies. Hydration time was confirmed as a significant factor influencing the entrapment efficiency of transfersomes, further affecting their transdermal flux in vitro. The fluxes of cinnamic acid from transfersomes were all higher than those from conventional liposomes, and the flux from the optimal transfersome formulation was 3.01-fold higher than that from the conventional liposomes (p?
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Acute ethanol administration inhibits Toll-like receptor 4 signaling pathway in rat intestinal epithelia.
Alcohol
PUBLISHED: 01-17-2013
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Excess alcohol intake, as in binge drinking, increases susceptibility to microbial pathogens. Alcohol impairs macrophage function by suppression of the Toll-like receptor 4 (TLR4) pathway. This study investigated the effects of acute ethanol intake on the TLR4 pathway in rat intestinal epithelia, which usually encounters luminal antigens at first and participates in the development of intestinal immunity. Twenty Wistar rats were randomly assigned to an ethanol group given ethanol as a 25% (v/v) solution in water at 7.5 g/kg, or a control group given saline, by oral gavage daily for 3 days. The epithelial histology and ultrastructure, the intestinal microflora, peripheral and portal venous plasma lipopolysaccharide (LPS) levels, and somatostatin (SST) levels in the peripheral plasma and small intestine were evaluated. Somatostatin receptor 2 (SSTR2), TLR4, TANK binding kinase-1 (TBK1), activated nuclear factor-?B (NF-?B), interferon-? (IFN-?) and tumor necrosis factor-? (TNF-?) in the intestinal mucosa were assayed. LPS responsiveness with or without SST pretreatment was assayed in vitro by quantification of TLR4, TBK1, activated NF-?B, IFN-? and TNF-? in isolated intestinal epithelia. Mucosal damage was observed in the ethanol group by light and electron microscopy. Escherichia coli cultures were unchanged in rat intestine of the ethanol group compared with controls, but lactobacilli cultures were reduced (p < 0.05). LPS levels increased in peripheral and portal venous plasma (p < 0.05), but mucosal TLR4, TBK1, nuclear NF-?B, IFN-? and TNF-? were unchanged in the ethanol group. LPS treatment in vitro up-regulated the level of TLR4, TBK1 and nuclear NF-?B as well as the production of IFN-? and TNF-? in isolated intestinal epithelia in the control (p < 0.05), but not the ethanol group. The stimulatory effects of LPS on intestinal epithelia isolated from the control group were significantly inhibited by SST pretreatment (p < 0.05). The peripheral plasma and intestinal levels of SST and the mucosal expression of SSTR2 in the ethanol group were significantly higher than in the control group (p < 0.05). These findings suggest the hyposensitivity of intestinal epithelial TLR4 to LPS induced by acute alcohol abuse probably through ethanol per se and ethanol-enhanced intestinal mucosal SST pathway may be a novel mechanism for increased susceptibility to intestinal pathogens.
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[Application of intraoperative ultrasound combined with neuronavigation for resection of intracranial cavernous malformations in minimally invasive neurosurgery].
Zhonghua Wai Ke Za Zhi
PUBLISHED: 12-16-2011
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To analyze the reliability and clinical value of intraoperative ultrasound combined with neuronavigation for resection of intracranial cavernous malformations.
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A new phenol compound from endophytic Phomopsis sp. DC01.
Nat. Prod. Res.
PUBLISHED: 11-22-2011
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The strain DC01 was isolated from the branch tissue of Daphniphyllum longeracemosum and determined to be a member of Phomopsis according to the ITS sequence analysis. The extracts from the PDA solid fermentation media of Phomopsis sp. DC01 were purified and three compounds including one new phenol compound were obtained. The new compound was identified to be (E?)-7-(2-hydroxy-4-(hydroxymethyl)phenyl)-2-methyloct-6-enoic acid (1) based on 1-D NMR, 2-D NMR and HR-ESI data.
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The effect of childbirth on carcinogenesis of DMBA-induced breast cancer in female SD rats.
Chin J Cancer
PUBLISHED: 11-01-2011
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Many epidemiologic and clinical studies have indicated that the frequency of breast cancer was lower in parous women than in nulliparous women. Moreover, the incidence of breast cancer has been reported to be lower in women with early childbirth than in women with late childbirth. To verify the effect of childbirth and the age at first childbirth on carcinogenesis and progression of breast cancer, we induced breast cancer by 7,12-dimethylbenanthracene (DMBA) in 120 female Sprague-Dawley (SD) rats, and divided them into control or experimental (DMBA-treated) nulliparous, early childbirth, and late childbirth groups to observe the incidence, latency, and size of breast cancer. Argyrophilic nucleolar organizer regions (AgNOR) count and the expression of C-erbB-2, proliferating cell nuclear antigen (PCNA), Ki-67, and minichromosome maintenance protein 2 (MCM2) in breast cancer tissues were detected by immunohistochemistry. The breast cancer incidences were 95.0%, 16.7%, and 58.8% in the experimental nulliparous, early childbirth, and late childbirth groups, respectively (all P < 0.05). Between any two of these groups, the latency was significantly different, but tumor size was similar. AgNOR count and the expression of C-erbB-2, PCNA, Ki-67, and MCM2 were significantly higher in the experimental nulliparous group than in the experimental early or late childbirth groups (P < 0.05), but no significant differences were observed between the latter two groups. Taken together, the results suggest that childbirth, especially early childbirth, can reduce the incidence and postpone the onset of DMBA-induced breast cancer.
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Enhanced transdermal delivery of evodiamine and rutaecarpine using microemulsion.
Int J Nanomedicine
PUBLISHED: 10-21-2011
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The purpose of this study was to improve skin permeation of evodiamine and rutaecarpine for transdermal delivery with microemulsion as vehicle and investigate real-time cutaneous absorption of the drugs via in vivo microdialysis.
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Evaluation outcomes of donors in living donor liver transplantation: a single-center analysis of 132 donors.
HBPD INT
PUBLISHED: 09-28-2011
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Donor safety has always been a major concern, and potential risk to the donor must be balanced against recipient benefit. However, lack of a standardized and uniform evaluation of perioperative complications is a serious limitation of the evaluation of donor morbidity. This study was designed to evaluate the outcomes of donors in adult living donor liver transplantation (LDLT) using the newer Clavien classification system in a single center in China.
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[Survey of clinical and experimental researches on mechanisms of acupuncture treatment of bronchial asthma].
Zhen Ci Yan Jiu
PUBLISHED: 09-28-2011
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In the present paper, the authors review the development of experimental and clinical studies on acupuncture treatment of bronchial asthma in recent 10 years. Regarding clinical studies, results showed that acupuncture could (1) regulate cardiac-pulmonary function; and (2) adjust immune state and relieve inflammatory reactions in bronchial asthma patients. Animal experiments showed that acupuncture could function in (1) improving pulmonary function; (2) reducing accumulation of the peripheral eosinophile granulocytes (EOS), relieving the infiltration of inflammatory cells in the air-passage mucosa and promoting the apoptosis of EOS in the lung and air-passages; (3) down-regulating the expression of air-passage remodeling-related protein insulin growth factor-1; (4) suppressing the secretion of tumor necrosis factor and endothelin; (5) attenuating allergic reaction; (6) regulating neuroendocrine activity; and (7) modulating intracellular second messenger activities. However, rigorous clinical study design is not enough, so that the reliability of the results is limited. In spite of many indicators of animal experiments have been selected, but their correlations are not in close association, resulting in poor complementation and mutual identification of the acquired findings. For this reason, its clinical efficacies need to be researched further according to principles of evidence-based medicine.
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Recurrence of a cerebral arteriovenous malformation following complete surgical resection: A case report and review of the literature.
Surg Neurol Int
PUBLISHED: 09-27-2011
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Angiography-confirmed complete resection of an arteriovenous malformation (AVM) has traditionally been considered curative. However, recurrence of AVM following angiographically proven complete resection does exist, especially in children. This rare occurrence has been reported 29 times in the English language literature. Although recurrence may be asymptomatic, many reported cases result in epilepsy or intracranial hemorrhage anywhere from 0.5 to 9 years following complete resection. We report a rare case of AVM recurrence that became symptomatic 16 years after complete resection. We review the literature and discuss the relevance of performing follow-up imaging to detect AVM recurrence.
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Experimental and computational study of the formation mechanism of the diammoniate of diborane: the role of dihydrogen bonds.
J. Am. Chem. Soc.
PUBLISHED: 08-19-2011
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The mechanism of formation of ammonia borane (NH(3)BH(3), AB) and the diammoniate of diborane ([H(2)B(NH(3))(2)][BH(4)], DADB) in the reaction between NH(3) and THF·BH(3) was explored experimentally and computationally. Ammonia diborane (NH(3)BH(2)(?-H)BH(3), AaDB), a long-sought intermediate proposed for the formation of DADB, was directly observed in the reaction using (11)B NMR spectroscopy. The results indicate that dihydrogen bonds between the initially formed AB and AaDB accelerate the formation of DADB in competition with the formation of AB.
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Microemulsion-based novel transdermal delivery system of tetramethylpyrazine: preparation and evaluation in vitro and in vivo.
Int J Nanomedicine
PUBLISHED: 08-09-2011
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To deliver 2,3,5,6-tetramethylpyrazine (TMP) in a relatively large dose through a transdermal route and facilitate the practical application of microemulison in transdermal drug delivery.
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Minimal incisions for treating zygomatic complex fractures.
J Craniofac Surg
PUBLISHED: 07-21-2011
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The objective was to study the clinical application and effects of minimal incisions for treating zygomatic complex (ZMC) fractures.
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[Clinical characteristics and surgical outcomes of 3094 patients with cerebral arteriovenous malformation].
Zhonghua Yi Xue Za Zhi
PUBLISHED: 07-18-2011
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To retrospectively analyze the clinical characteristics and surgical outcomes of 3094 patients with cerebral arteriovenous malformation (AVM) over a course of 53 years.
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[The application of functional magnetic resonance imaging integrated neuronavigation in localization and lingual function protection].
Zhonghua Yi Xue Za Zhi
PUBLISHED: 06-29-2011
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To evaluate the clinical practicability of integration of functional magnetic resonance imaging (fMRI) data into neuronavigation as a tool to localize the language area and their relationship with the lesion for the preoperative planning, intraoperative guidance and postoperative follow-up study of brain functions during minimally invasive surgeries in or adjacent to functional areas.
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[Clinical characteristics and surgical outcomes of intracranial aneurysm: a retrospective study of 3322 cases].
Zhonghua Yi Xue Za Zhi
PUBLISHED: 06-29-2011
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To preliminarily explore the clinical characteristics and surgical outcomes of intracranial aneurysm.
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Higher tacrolimus blood concentration is related to hyperlipidemia in living donor liver transplantation recipients.
Dig. Dis. Sci.
PUBLISHED: 06-08-2011
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The arrival of tacrolimus has drastically improved AALDLT recipients survival. However, little data of tacrolimus have been reported concerning its effects on lipid metabolism for AALDLT recipients.
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Deletion of insulin-degrading enzyme elicits antipodal, age-dependent effects on glucose and insulin tolerance.
PLoS ONE
PUBLISHED: 05-12-2011
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Insulin-degrading enzyme (IDE) is widely recognized as the principal protease responsible for the clearance and inactivation of insulin, but its role in glycemic control in vivo is poorly understood. We present here the first longitudinal characterization, to our knowledge, of glucose regulation in mice with pancellular deletion of the IDE gene (IDE-KO mice).
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[Intermediate structure of the interior-exterior relationship between lung and large intestine].
Zhongguo Zhen Jiu
PUBLISHED: 05-03-2011
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Through analysis and study of ancient literatures, it is primarily approved that there existed a connective subject, namely intermediate structure, between the "lung-lung meridian" and the "large intestine-large intestine meridian", which can promote the interior-exterior close relationship between the two. It mainly includes intermediate of the organism (throat, supraclavicular fossa, the bone formed elbow joint, nose, anus and skin), intermediate of acupoints (Luo-connecting points) and intermediate of zang-fu, meridians and collaterals (stomach and the Stomach Meridian of Foot-Yangming, liver and Liver Meridian of Foot-Jueyin). The theory provides new thoughts for study on the interior-exterior relationship between the corresponding zang and fu organs.
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Efficacy of acupuncture for migraine prophylaxis: a single-blinded, double-dummy, randomized controlled trial.
Pain
PUBLISHED: 03-25-2011
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Insufficient clinical trial data were available to prove the efficacy of acupuncture for migraine prophylaxis. A multicenter, double-dummy, single-blinded, randomized controlled clinical trial was conducted at the outpatient departments of acupuncture at 5 hospitals in China to evaluate the effectiveness of acupuncture. A total of 140 patients with migraine without aura were recruited and assigned randomly to 2 different groups: the acupuncture group treated with verum acupuncture plus placebo and the control group treated with sham acupuncture plus flunarizine. Treated by acupuncture 3 times per week and drugs every night, patients from both groups were evaluated at week 0 (baseline), week 4, and week 16. The primary outcome was measured by the proportion of responders (defined as the proportion of patients with a reduction of migraine days by at least 50%). The secondary outcome measures included the number of migraine days, visual analogue scale (VAS, 0 to 10 cm) for pain, as well as the physical and mental component summary scores of the 36-item short-form health survey (SF-36). The patients in the acupuncture group had better responder rates and fewer migraine days compared with the control group (P<.05), whereas there were no significant differences between the 2 groups in VAS scores and SF-36 physical and mental component summary scores (P>.05). The results suggested that acupuncture was more effective than flunarizine in decreasing days of migraine attacks, whereas no significantly differences were found between acupuncture and flunarizine in reduction of pain intensity and improvement of the quality of life.
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[Clinical study of patients of arachnoid cyst associated with chronic subdural hematoma].
Zhonghua Yi Xue Za Zhi
PUBLISHED: 03-23-2011
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To explore the clinical characteristics and treatment strategy of arachnoid cyst associated with chronic subdural hematoma.
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Ammonium octahydrotriborate (NH4B3H8): new synthesis, structure, and hydrolytic hydrogen release.
Inorg Chem
PUBLISHED: 03-15-2011
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A metathesis reaction between unsolvated NaB(3)H(8) and NH(4)Cl provides a simple and high-yield synthesis of NH(4)B(3)H(8). Structure determination through X-ray single crystal diffraction analysis reveals weak N-H(?+)---H(?-)-B interaction in NH(4)B(3)H(8) and strong N-H(?+)---H(?-)-B interaction in NH(4)B(3)H(8)·18-crown-6·THF adduct. Pyrolysis of NH(4)B(3)H(8) leads to the formation of hydrogen gas with appreciable amounts of other volatile boranes below 160 °C. Hydrolysis experiments show that upon addition of catalysts, NH(4)B(3)H(8) releases up to 7.5 materials wt % hydrogen.
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Quantification and location of Chlamydia pneumoniae-specific antigen in the walls of abdominal aortic aneurysms.
Ann Vasc Surg
PUBLISHED: 02-15-2011
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To evaluate the prevalence and quantity of Chlamydia pneumoniae-specific antigen in the three layers (intima, media, and adventitia) of abdominal aortic aneurysms (AAAs), so as to further investigate the pathogenesis of AAAs.
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What is Visualize?

JoVE Visualize is a tool created to match the last 5 years of PubMed publications to methods in JoVE's video library.

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We use abstracts found on PubMed and match them to JoVE videos to create a list of 10 to 30 related methods videos.

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In developing our video relationships, we compare around 5 million PubMed articles to our library of over 4,500 methods videos. In some cases the language used in the PubMed abstracts makes matching that content to a JoVE video difficult. In other cases, there happens not to be any content in our video library that is relevant to the topic of a given abstract. In these cases, our algorithms are trying their best to display videos with relevant content, which can sometimes result in matched videos with only a slight relation.