JoVE Visualize What is visualize?
Stop Reading. Start Watching.
Advanced Search
Stop Reading. Start Watching.
Regular Search
Find video protocols related to scientific articles indexed in Pubmed.
Impact of STAT3 Inhibition on Survival of Osteosarcoma Cell Lines.
Anticancer Res.
PUBLISHED: 11-05-2014
Show Abstract
Hide Abstract
Osteosarcoma is often a fatal malignancy. Constitutive STAT3 activation is associated with various human cancers and commonly suggests poor prognosis. We aimed to investigate the effect and potential molecular mechanisms of STAT3 inhibition on osteosarcoma.
Related JoVE Video
Determination of the Enthalpy of Vaporization and Prediction of Surface Tension for Ionic Liquid 1-Alkyl-3-methylimidazolium Propionate [Cnmim][Pro](n = 4, 5, 6).
J Phys Chem B
PUBLISHED: 11-05-2014
Show Abstract
Hide Abstract
With the use of isothermogravimetrical analysis, the enthalpies of vaporization, ?(g)lH(o)m(Tav), at the average temperature, Tav = 445.65 K, for the ionic liquids (ILs) 1-alkyl-3-methylimidazolium propionate [Cnmim][Pro](n = 4, 5, 6) were determined. Using Verevkin's method, the difference of heat capacities between the vapor phase and the liquid phase, ?(g)lCp(o)m, for [Cnmim][Pro](n = 2, 3, 4, 5, 6), were calculated based on the statistical thermodynamics. Therefore, with the use of ?(g)lCp(o)m, the values of ?(g)lH(o)m(Tav) were transformed into ?(g)lH(o)m(298), 126.8, 130.3, and 136.5 for [Cnmim][Pro](n = 4, 5, 6), respectively. In terms of the new scale of polarity for ILs, the order of the polarity of [Cnmim][Pro](n = 2, 3, 4, 5, 6) was predicted, that is, the polarity decreases with increasing methylene. A new model of the relationship between the surface tension and the enthalpy of vaporization for aprotic ILs was put forward and used to predict the surface tension for [Cnmim][Pro](n = 2, 3, 4, 5, 6) and others. The predicted surface tension for the ILs is in good agreement with the experimental one.
Related JoVE Video
Factors Associated With Early Mortality After Living-Donor Liver Transplant.
Exp Clin Transplant
PUBLISHED: 10-25-2014
Show Abstract
Hide Abstract
We sought to identify the risk factors associated with the early mortality after a living-donor liver transplant.
Related JoVE Video
A 4-way wavelength demultiplexer based on the plasmonic broadband slow wave system.
Opt Express
PUBLISHED: 10-17-2014
Show Abstract
Hide Abstract
We propose a broadband slow wave system based on the thin metal-insulator-metal (MIM) graded grating structure composed of two corrugated metal strips with periodic array of grooves on a thin dielectric substrate. The guided spoof surface plasmon polaritons (SSPPs) at different frequencies can be localized at different positions along the ultrathin MIM grating. By introducing specially designed non-corrugated MIM branches with specific lengths at the locations where the EM waves are trapped, the trapped EM waves can be released and propagate along these branches. A 4-way wavelength demultiplexer based on such plasmonic broadband slow wave system is then demonstrated and fabricated. To improve the isolations between different branches at lower frequencies, band-reject filters are inserted at the front of some MIM branches. The measurements and the simulation results have shown very good agreements, which validate the feasibility of the 4-way wavelength demultiplexer.
Related JoVE Video
Is hepatectomy for huge hepatocellular carcinoma (? 10 cm in diameter) safe and effective? A single-center experience.
Asian Pac. J. Cancer Prev.
PUBLISHED: 09-18-2014
Show Abstract
Hide Abstract
This retrospective study aimed to validate the safety and effectiveness of hepatectomy for huge hepatocellular carcinoma (HCC).
Related JoVE Video
[Research on glucose measuring technique by surface plasmon resonance based on thiol coupling].
Guang Pu Xue Yu Guang Pu Fen Xi
PUBLISHED: 09-12-2014
Show Abstract
Hide Abstract
In the glucose measuring technique by surface plasmon resonance, D-galactose/D-glucose binding protein (GGBP) that can specifically adsorb glucose was introduced, and high-precision specific detection of glucose concentration was realized. In the present paper, the GGBP protein was bound on the surface of SPR sensor through thiol coupling method. GGBP binding experiment was carried out on SPR sensor and then glucose concentration experiment was conducted with this sensor. The results indicated that the SPR sensor had good linearity, stability and repeatability in the range of 0.1-10 mg x dL(-1). SPR sensor bound with GGBP would have great potential and vast development prospects.
Related JoVE Video
The relationship between the plant-encoded RNA-dependent RNA polymerase 1 and alternative oxidase in tomato basal defense against Tobacco mosaic virus.
Planta
PUBLISHED: 09-05-2014
Show Abstract
Hide Abstract
Salicylic acid (SA) plays a critical role in plant defense against pathogen attack. The SA-induced viral defense in plants is distinct from the pathways mediating bacterial and fungal defense, which is pathogenesis-related protein-independent but involves an RNA-dependent RNA polymerase 1 (RDR1)-mediated RNA silencing mechanism and/or an alternative oxidase (AOX)-associated defense pathway. However, the relationship between these two viral defense-related pathways remains unclear. In this study, Tobacco mosaic virus (TMV) inoculation onto Solanum lycopersicum (tomato) leaves induced a rapid induction of the SlAOX1a transcript level as well as the total and CN-resistant respiration at 0.5 dpi, followed by an increase in SlRDR1 gene expression at 1 dpi in the upper uninoculated leaves. Silencing SlRDR1 using virus-induced gene silencing system significantly reduced SlRDR1 expression and tomato defense against TMV but had no evident effect on SlAOX1a transcription. Conversely, silencing SlAOX1a not only effectively reduced the AOX1a transcript level, but also blocked the TMV-induced SlRDR1 expression and decreased the basal defense against TMV. Furthermore, the application of an exogenous AOX activator on empty vector-silenced control plants greatly induced the accumulation of SlRDR1 and SlAOX1a transcript and reduced TMV viral RNA accumulation, but failed to have such effects on SlRDR1-silenced plants. Moreover, RDR1-overexpressed transgenic Nicotiana benthamiana plants enhanced defense against TMV than the empty vector-transformed plants, but these effects were not affected by the exogenous AOX activator or inhibitor. These results indicate that RDR1 is involved in the AOX-mediated defense pathway against TMV infection and plays a crucial role in enhancing RNA silencing to limit virus systemic spread.
Related JoVE Video
Co-molten solvothermal method for synthesizing chalcopyrite CuFe(1-x)Cr(x)S? (x ? 0.4): high photocatalytic activity for the reduction of nitrate ions.
Dalton Trans
PUBLISHED: 09-05-2014
Show Abstract
Hide Abstract
In literature, it is very difficult to obtain sulfides with Cr(3+) in tetrahedral coordination. Here, a thiourea-oxalic acid co-molten solvothermal method was applied to synthesize chalcopyrite CuFe(1-x)Cr(x)S2 (x ? 0.4) solid solutions. We propose that oxalic acid plays an important role in the crystallization of CuFe(1-x)Cr(x)S2 and can considerably restrain the formation of other undesirable impurities. The successful incorporation of Cr(3+) was confirmed by powder XRD, SEM and EDX mapping (2D elemental distribution). The UV-Vis reflectance spectra of CuFe(1-x)Cr(x)S2 suggest that the bandgap energies decrease from 0.80 to 0.61 eV along with an increase in the Cr(3+) concentration. All the CuFe(1-x)Cr(x)S2 (0 ? x ? 0.4) samples show considerably higher photocatalytic activities than P25 toward the reduction of nitrate ions in aqueous solution. We speculate that the thiourea-oxalic acid co-molten method may not only be effective to synthesize Fe(3+)-Cr(3+) sulfides, but can also be helpful to incorporate Cr(3+) to other sulfide systems with MS4 tetrahedra.
Related JoVE Video
Efficacy of surgical resection for pulmonary metastases from hepatocellular carcinoma.
Med. Sci. Monit.
PUBLISHED: 08-30-2014
Show Abstract
Hide Abstract
The lung is one of the most common sites for extrahepatic metastasis from hepatocellular carcinoma (HCC). This study aimed to assess the efficacy of surgical resection for pulmonary metastases from HCC.
Related JoVE Video
Current clinical regulation of PI3K/PTEN/Akt/mTOR signalling in treatment of human cancer.
J. Cancer Res. Clin. Oncol.
PUBLISHED: 08-22-2014
Show Abstract
Hide Abstract
PTEN is an essential tumour suppressor gene which encodes a phosphatase protein that antagonises the PI3K/Akt/mTOR antiapoptotic pathway. Impairment of this tumour suppressor pathway potentially becomes a causal factor for development of malignancies. This review aims to assess current understanding of mechanisms of dysfunction involving the PI3K/PTEN/Akt/mTOR pathway linked to tumorigenesis and evaluate the evidence for targeted therapy directed at this signalling axis.
Related JoVE Video
Prognosis evaluation in patients with hepatocellular carcinoma after hepatectomy: comparison of BCLC, TNM and Hangzhou criteria staging systems.
PLoS ONE
PUBLISHED: 08-18-2014
Show Abstract
Hide Abstract
This study is to evaluate the Hangzhou criteria (HC) for patients with HCC undergoing surgical resection and to identify whether this staging system is superior to other staging systems in predicting the survival of resectable HCC.
Related JoVE Video
Eyes on the bodies: An eye tracking study on deployment of visual attention among females with body dissatisfaction.
Eat Behav
PUBLISHED: 08-17-2014
Show Abstract
Hide Abstract
Visual attentional bias has important functions during the appearance social comparisons. However, for the limitations of experimental paradigms or analysis methods in previous studies, the time course of attentional bias to thin and fat body images among women with body dissatisfaction (BD) has still been unclear. In using free reviewing task combined with eye movement tracking, and based on event-related analyses of the critical first eye movement events, as well as epoch-related analyses of gaze durations, the current study investigated different attentional bias components to body shape/part images during 15s presentation time among 34 high BD and 34 non-BD young women. In comparison to the controls, women with BD showed sustained maintenance biases on thin and fat body images during both early automatic and late strategic processing stages. This study highlights a clear need for research on the dynamics of attentional biases related to body image and eating disturbances.
Related JoVE Video
[Effects of controllable dynamic inhaled exposure of moxa smoke on LDL-r, ICAM-1 and morphology of heart tissue in rats].
Zhongguo Zhen Jiu
PUBLISHED: 08-13-2014
Show Abstract
Hide Abstract
To observe the change of lipid metabolism and vascular endothelium as well as morphology of heart tissue in rats who were long-time exposed to moxa smoke with different concentrations in order to provide reference for safety assessment of moxa smoke on cardiovascular system.
Related JoVE Video
A genomic survey of thirty soybean-infecting bean common mosaic virus (BCMV) isolates from China pointed BCMV as a potential threat to soybean production.
Virus Res.
PUBLISHED: 08-05-2014
Show Abstract
Hide Abstract
Widely known as a severe pathogen of bean plants, the bean common mosaic virus (BCMV) has been reported to infect soybeans only sporadically and the involved strains were all found in China regions. To explore variations among soybean-infecting BCMV strains, hundreds of soybean mosaic leave samples were collected throughout China, with a total of 30 BCMV isolates detected and their genomes sequenced. These newly obtained genomes, together with 16 other BCMV genomes available in GenBank were examined from multiple aspects to characterize BCMV evolutionary processes. Phylogenetic analysis showed that both soybean-infecting BCMVs (group I) and peanut-infecting BCMVs (group II) are distantly related to other BCMVs, suggesting ancestral differentiation and host adaptation. Genetic variation analysis showed that P1, P3 and 6K2 genes and the beginning portion of CP gene showed higher levels of variation relative to other genes. Moreover, selection analyses further confirmed that a number of sites within the P1 and P3 genes have suffered positive selection. These obtained BCMV sequences also exhibit high recombination frequencies, indicating a more dynamic evolutionary history. Finally, 12 different soybean cultivars were challenged with two BCMV isolates (DXH015 and HZZB011), with most of the cultivars successfully infected. These findings suggest that BCMV is indeed a potential threat to soybean production.
Related JoVE Video
Recovery of absolute height from wrapped phase maps for fringe projection profilometry.
Opt Express
PUBLISHED: 08-05-2014
Show Abstract
Hide Abstract
A novel multi-frequency fringe projection profilometry is presented in this paper. Fringe patterns with multiple frequencies are projected onto an object by a digital micro-mirror device projector. The approach involves an improved Fourier transform profilometry method with an additional ? phase shifting stage and hence the acquisition of two source images. A peak searching algorithm is then employed to obtain the real height profile of the object together with a mathematical proof of this algorithm. In our method, the height of each point on the object is measured independently and the phase unwrapping procedure is avoided, enabling the measurement of objects with large depth discontinuities, where the phase unwrapping is difficult. The measurement result is given to validate the method in the paper. Our technique has great potential in industrial applications where the measurement of objects with complex shape and large discontinuities is needed.
Related JoVE Video
Enhanced water-soluble derivative of PC407 as a novel potential COX-2 inhibitor injectable formulation.
Bioorg. Med. Chem. Lett.
PUBLISHED: 07-25-2014
Show Abstract
Hide Abstract
PC407 is an effective COX-2 inhibitor in non-steroidal anti-inflammatory drug development but the poor solubility limits their usefulness. The aim of the study was to prepare and evaluate 4-oxo-4-[4-(5-(naphthalen-2-yl)-3-(trifluoromethyl)-1H-pyrazol-1-yl)benzenesulfonamido]butyrate disodium, a derivative of PC407 with enhanced water solubility for injectable formulation. The prepared derivative displayed interesting high aqueous solubility (20.3mg/mL, much superior to the parent compound PC407, 1.6?g/mL) with confirmed in vivo analgesic activity. This derivative represents the profiles of prodrug and potential candidate of PC407 for the development of injectable COX-2 inhibitor due to extraordinary water solubility, low toxicity, and impressive analgesic activity.
Related JoVE Video
Vitamin C protected human retinal pigmented epithelium from oxidant injury depending on regulating SIRT1.
ScientificWorldJournal
PUBLISHED: 07-22-2014
Show Abstract
Hide Abstract
The purpose was to investigate the protective effects of Vitamin C (Vit C) and the regulatory mechanism between Vit C and sirtuin 1 (SIRT1) in PREs during oxidative stress as Vit C and SIRT1 exerted famous effects as antioxidants. We found that moderate Vit C (100 µM) prevented ARPE-19 cells from damages induced by H2O2, including increasing viability, reducing apoptosis, and attenuating intracellular ROS levels. But lower and higher concentration of Vit C had no effects. Further results indicated that Vit C caused the dysregulation of some stress responses factors (SIRT1, p53 and FOXO3) in ARPE-19 cells response to H2O2. Moreover we found that SIRT1 activator resveratrol (SRV) stimulated significantly the protective effects of moderate Vit C, provided the property of antioxidative stress for the lower and higher concentration of Vit C in ARPE-19 cells as well. Consistently, nicotinamide (NA) relieved the protective functions of moderate Vit C. Interestingly, data also revealed the dysregulation of p53 and FOXO3 was dependent on the regulation of SIRT1 rather than Vit C. Summarily, the protective effect of Vit C against oxidative stress was involved in regulation of SIRT1. It suggested that combined application of Vit C and RSV might be a promising therapeutic method for AMD.
Related JoVE Video
Preparation and hydrophobicity of biomorphic ZnO/carbon based on a lotus-leaf template.
Mater Sci Eng C Mater Biol Appl
PUBLISHED: 07-10-2014
Show Abstract
Hide Abstract
A lotus leaf was used as a template to fabricate superhydrophobic biomorphic ZnO on a carbon substrate (ZnO/C). First, a carbon substrate with the microstructure of a leaf surface was obtained by sintering a lotus leaf in argon. A biomorphic ZnO/C material was then obtained by immersing this carbon substrate into a Zn(NO3)2 solution and sintering. Finally, the hydrophobicity of the surfaces of the products thus obtained was investigated. This ZnO/C material exhibited excellent superhydrophobicity and low adhesion after it was modified with fluorine silane. The water contact angle of the resulting product was 162°, which exceeds that of the lotus leaf (157°) and is much higher than that of smooth carbon covered with the same fluorine silane (114°). Moreover, this surface displayed a stable superhydrophobic performance even after exposure to ambient air for several months.
Related JoVE Video
Hepatic resection for multiple hepatocellular carcinoma less than 5 cm: a prospective comparative study.
Hepatogastroenterology
PUBLISHED: 06-05-2014
Show Abstract
Hide Abstract
Treatment of multiple hepatocellular carcinoma (HCC) remains a critical issue. In addition, the prognosis and prognostic factors of multiple HCC after hepatic resection are rarely prospectively documented.
Related JoVE Video
Corrosion resistance, surface evaluation, and geometric design comparison of five self-expanding nitinol stents used in clinical practice.
J. Endovasc. Ther.
PUBLISHED: 04-24-2014
Show Abstract
Hide Abstract
To investigate the corrosion resistance properties of 5 commercially available nitinol stents used to treat peripheral artery disease and compare their surface quality, elemental composition, and geometrical design.
Related JoVE Video
LRRC19 expressed in the kidney induces TRAF2/6-mediated signals to prevent infection by uropathogenic bacteria.
Nat Commun
PUBLISHED: 04-01-2014
Show Abstract
Hide Abstract
The innate immune-dependent bactericidal effects are critical for preventing microbial colonization in the urinary system. However, the mechanisms involved in establishing innate immune responses in kidney are not completely understood. Here we describe the role of a novel member of the LRR (leucine-rich repeat) class of transmembrane proteins, LRRC19 (LRR-containing 19) in eliminating uropathogenic bacteria. LRRC19 is predominantly expressed in human and mouse kidney tubular epithelial cells and LRRC19-deficient mice are more susceptible to uropathogenic Escherichia coli (UPEC) infection than wild-type or TLR4 knockout mice. Recognition of UPEC by LRRC19 induces the production of cytokines, chemokines and antimicrobial substances through TRAF2- and TRAF6-mediated NF-?B and MAPK signalling pathways. Thus, LRRC19 may be a critical pathogen-recognition receptor in kidney mediating the elimination of UPEC infection.
Related JoVE Video
Histopathologic validation of 3'-deoxy-3'-18F-fluorothymidine PET for detecting tumor repopulation during fractionated radiotherapy of human FaDu squamous cell carcinoma in nude mice.
Radiother Oncol
PUBLISHED: 03-31-2014
Show Abstract
Hide Abstract
FaDu human squamous cell carcinoma (FaDu-hSCC) demonstrates accelerated tumor repopulation during fractionated irradiation with pathological validation (Ki-67 and BrdUrd makers) in a xenograft model system. However, these and other functional assays must be performed ex vivo and post hoc. We propose a novel, in vivo, real-time assay utilizing (18)F-FLT PET.
Related JoVE Video
Efficacy of neoadjuvant cisplatin and 5-flourouracil prior to surgery in FIGO stage IB2/IIA2 cervical cancer.
Mol Clin Oncol
PUBLISHED: 03-21-2014
Show Abstract
Hide Abstract
Cervical cancer is currently the first or second leading cause of cancer-related mortality among women in developing countries. This study was conducted in order to determine whether neoadjuvant cisplatin and 5-flourouracil (NAPF) prior to surgery is superior to primary surgical treatment (PST) as a treatment option for patients with International Federation of Gynaecology and Obstetrics (FIGO) stage IB2/IIA2 cervical cancer. A retrospective review of 195 patients with early-stage bulky cervical cancer was performed. The patients were divided into two groups, according to whether they received NAPF prior to surgery. The surgical profiles and complications, risk factors of recurrence and survival were compared between the groups. The response rate to NAPF was found to be 61.2%. There were no differences in operative time and intra-operative complications between the two groups, whereas the estimated blood loss in the NAPF and PST groups were 620.1±394.9 and 434.8±233.7 ml, respectively (P=0.000). When compared with PST, NAPF remarkably reduced tumor size (22.5 vs. 93.3%, P=0.000). Furthemore, the ratio of deep stromal invasion was significantly lower in responders to NAPF compared with that in non-responders (46.7 vs. 76.3%, respectively; P=0.004) and in the PST group (46.7 vs. 70.0%, respectively; P=0.004). No reduction of high-risk factors (HRFs) was observed. The NAPF group, even the responder subgroup, exhibited no significant improvement in progression-free survival (PFS) and overall survival (OS) compared to the PST group. In conclusion, despite the reduction of intermediate-risk factors (IRFs), neoadjuvant chemotherapy (NAC) with the NAPF regimen prior to radical surgery (RS) did not improve the prognosis in patients with FIGO stage IB2/IIA2 cervical cancer.
Related JoVE Video
Living donor liver transplantation does not increase tumor recurrence of hepatocellular carcinoma compared to deceased donor transplantation.
World J. Gastroenterol.
PUBLISHED: 03-15-2014
Show Abstract
Hide Abstract
To compare the recurrence-free survival (RFS) and overall survival (OS) of hepatitis B virus (HBV)-positive hepatocellular carcinoma (HCC) after living donor liver transplantation (LDLT) and deceased donor liver transplantation (DDLT).
Related JoVE Video
Liver resection in hepatitis B related-hepatocellular carcinoma: clinical outcomes and safety in elderly patients.
World J. Gastroenterol.
PUBLISHED: 03-08-2014
Show Abstract
Hide Abstract
To compare the morbidity and mortality in young and elderly hepatocellular carcinoma (HCC) patients undergoing liver resection.
Related JoVE Video
Prognosis after resection of hepatitis B virus-related hepatocellular carcinoma originating from non-cirrhotic liver.
Ann. Surg. Oncol.
PUBLISHED: 02-28-2014
Show Abstract
Hide Abstract
Long-term prognosis after resection of hepatitis B virus (HBV)-related hepatocellular carcinoma (HCC) originating from non-cirrhotic liver is not fully clarified.
Related JoVE Video
Repopulation of tumor cells during fractionated radiotherapy and detection methods (Review).
Oncol Lett
PUBLISHED: 02-26-2014
Show Abstract
Hide Abstract
Repopulation of tumor cells during radiotherapy is believed to be a significant cause for treatment failure. The phenomenon of tumor repopulation during fractionated radiotherapy was found from clinical observations that identified that the local control rate decreased with a prolonged treatment time. A series of animal experiments with varied overall treatment time and fractionated doses were performed to demonstrate tumor cell repopulation during radiotherapy in various mouse xenograft models. However, conventional detection methods are challenging, as it is difficult to separate viable cells from those destined for apoptosis during fractionated radiotherapy. In essence, the mechanism of tumor repopulation involves the continuing proliferation of clonogenic tumor cells. In vivo imaging, tracking and targeting of the repopulation of these cells has been of clinical interest so as to administer a higher dose to the tumor repopulation regions. Currently, functional imaging methods, including 3'-deoxy-3'-(18)F-fluorothymidine positron emission tomography ((18)F-FLT PET), are showing promise in assessing the proliferation activity of tumors in vivo. This review mainly focuses on the phenomenon of tumor repopulation during radiotherapy and its conventional and novel detection methods, particularly on the feasibility of (18)F-FLT PET for the detection of tumor-cell repopulation.
Related JoVE Video
Effect of celecoxib on inhibiting tumor repopulation during radiotherapy in human FaDu squamous cell carcinoma.
Contemp Oncol (Pozn)
PUBLISHED: 02-14-2014
Show Abstract
Hide Abstract
FaDu human squamous cell carcinoma (FaDu-hSCC) demonstrated accelerated tumor repopulation during fractionated irradiation with pathological validation in a xenograft model system. Previous studies showed that the selective cyclooxygenase (COX)-2 inhibitor celecoxib can enhance the tumor response to radiotherapy. So we aimed to explore the effect of celecoxib in inducing apoptosis and inhibiting repopulation of FaDu tumors in nude mice during fractionated radiotherapy.
Related JoVE Video
Risk factors for combined hepatocellular-cholangiocarcinoma: a hospital-based case-control study.
World J. Gastroenterol.
PUBLISHED: 02-14-2014
Show Abstract
Hide Abstract
To identify risk factors contributing to the development of combined hepatocellular-cholangiocarcinoma (CHC) in China.
Related JoVE Video
Open-framework gallium borate with boric and metaboric acid molecules inside structural channels showing photocatalysis to water splitting.
Inorg Chem
PUBLISHED: 02-10-2014
Show Abstract
Hide Abstract
An open-framework gallium borate with intrinsic photocatalytic activities to water splitting has been discovered. Small inorganic molecules, H3BO3 and H3B3O6, are confined inside structural channels by multiple hydrogen bonds. It is the first example to experimentally show the structural template effect of boric acid in flux synthesis.
Related JoVE Video
Child-Pugh A hepatitis B-related cirrhotic patients with a single hepatocellular carcinoma up to 5 cm: liver transplantation vs. resection.
J. Gastrointest. Surg.
PUBLISHED: 02-03-2014
Show Abstract
Hide Abstract
This study aims to analyze the outcomes of patients with Child-Pugh A class cirrhosis and a single hepatocellular carcinoma (HCC) up to 5 cm in diameter who underwent liver transplantation vs. resection.
Related JoVE Video
Neutrophil to lymphocyte ratio changes predict small hepatocellular carcinoma survival.
J. Surg. Res.
PUBLISHED: 01-28-2014
Show Abstract
Hide Abstract
There is limited information available concerning the delta neutrophil to lymphocyte ratio (?NLR) in hepatocellular carcinoma (HCC). The present study was designed to evaluate the predictive value of dynamic change of NLR in patients who undergo curative resection for small HCC.
Related JoVE Video
Low immediate postoperative platelet count is associated with hepatic insufficiency after hepatectomy.
World J. Gastroenterol.
PUBLISHED: 01-26-2014
Show Abstract
Hide Abstract
To investigate the relationship between low immediate postoperative platelet count and perioperative outcome after liver resection in patients with hepatocellular carcinoma (HCC).
Related JoVE Video
Individual identification of Chinese Holstein bull by 10 STR loci.
Mol. Biol. Rep.
PUBLISHED: 01-22-2014
Show Abstract
Hide Abstract
With the premium bull plays a growing important role in cattle industry, semen detection technology based on individual identification and phylogenetic relationship is paid more and more attention. In order to lay the foundation for the establishment of the China Holstein bull identification method, this research takes 20 Chinese Holstein dairy bull's blood and their corresponding semen, and then extracts the DNA both from the blood and semen, analysis the genetic polymorphisms of 10 microsatellite loci (TGLA227, INRA23, TGLA122, BM2113, SPS115, ETH3, ETH225, MCM158, MAF45 and UMN0108) by microsatellite marker, discuss the feasibility of this method used to individual identification. The results showed that Chinese Holstein dairy bull genetic diversity in the ten microsatellite loci were both high, and the average polymorphic information content of TGLA227, which highest, is 0.8162, ETH225 has the lowest, which is 0.6224. Use STR loci to identify the bull's semen, the cumulative individual identification capacity is 99.99%, which indication that 10 STR loci can be used to the frozen semen quality test and cows individual identification.
Related JoVE Video
Effect of low-dose tacrolimus with mycophenolate mofetil on renal function following liver transplantation.
World J. Gastroenterol.
PUBLISHED: 01-12-2014
Show Abstract
Hide Abstract
To determine whether low-dose tacrolimus (TAC) combined with mycophenolate mofetil (MMF) is a safe approach to decrease the incidence of chronic kidney disease (CKD) in liver transplantation (LT) recipients.
Related JoVE Video
Effect of piracetam on the cognitive performance of patients undergoing coronary bypass surgery: A meta-analysis.
Exp Ther Med
PUBLISHED: 01-08-2014
Show Abstract
Hide Abstract
Cognitive impairments are observed in numerous patients following coronary bypass surgery, and piracetam are nootropic compounds that modulate cerebral functions by directly enhancing cognitive processes. The present meta-analysis was conducted to evaluate the protective effect of piracetam on the cognitive performance of patients undergoing coronary bypass surgery. The relevant studies were identified by searching Medline, EMBASE, PubMed and the Cochrane Library up to June 2013 and the pertinent bibliographies from the retrieved studies were reviewed. Data were selected from the studies according to predefined criteria. The meta-analysis included two randomized control trials involving 184 patients and including the Syndrom-Kurz test (SKT). Findings of the meta-analysis showed that following treatment the change from baseline observed in five SKT subtest scores, conducted with piracetam patients, indicated a significant advantage over those patients that were in the placebo group. The subtests included immediate pictured object recall, weighted mean difference (WMD)=0.91, 95% confidence interval (CI) 0.51-1.31, P<0.00001; delayed pictured object recall, WMD=0.74, 95% CI 0.19-1.28, P=0.008; delayed picture recognition, WMD=0.82, 95% CI 0.31-1.31, P=0.001; immediate word recall, WMD=0.87, 95% CI 0.47-1.28, P<0.0001; and letter interference, WMD=3.46, 95% CI -5.69 to -1.23, P=0.002. These results indicated that piracetam may have been effective in improving the short-term cognitive performance of patients undergoing coronary bypass surgery. High quality, well-controlled and longer randomized trials are required to corroborate this result.
Related JoVE Video
The value of magnetic resonance imaging in the detection of prostate cancer in patients with previous negative biopsies and elevated prostate-specific antigen levels: a meta-analysis.
Acad Radiol
PUBLISHED: 01-07-2014
Show Abstract
Hide Abstract
To assess the diagnostic performance of magnetic resonance imaging (MRI) for targeting prostate cancer in patients with previous negative biopsies and elevated prostate-specific antigen (PSA) levels.
Related JoVE Video
Complex Spatiotemporal Responses of Global Terrestrial Primary Production to Climate Change and Increasing Atmospheric CO2 in the 21st Century.
PLoS ONE
PUBLISHED: 01-01-2014
Show Abstract
Hide Abstract
Quantitative information on the response of global terrestrial net primary production (NPP) to climate change and increasing atmospheric CO2 is essential for climate change adaptation and mitigation in the 21st century. Using a process-based ecosystem model (the Dynamic Land Ecosystem Model, DLEM), we quantified the magnitude and spatiotemporal variations of contemporary (2000s) global NPP, and projected its potential responses to climate and CO2 changes in the 21st century under the Special Report on Emission Scenarios (SRES) A2 and B1 of Intergovernmental Panel on Climate Change (IPCC). We estimated a global terrestrial NPP of 54.6 (52.8-56.4) PgC yr-1 as a result of multiple factors during 2000-2009. Climate change would either reduce global NPP (4.6%) under the A2 scenario or slightly enhance NPP (2.2%) under the B1 scenario during 2010-2099. In response to climate change, global NPP would first increase until surface air temperature increases by 1.5°C (until the 2030s) and then level-off or decline after it increases by more than 1.5°C (after the 2030s). This result supports the Copenhagen Accord Acknowledgement, which states that staying below 2°C may not be sufficient and the need to potentially aim for staying below 1.5°C. The CO2 fertilization effect would result in a 12%-13.9% increase in global NPP during the 21st century. The relative CO2 fertilization effect, i.e. change in NPP on per CO2 (ppm) bases, is projected to first increase quickly then level off in the 2070s and even decline by the end of the 2080s, possibly due to CO2 saturation and nutrient limitation. Terrestrial NPP responses to climate change and elevated atmospheric CO2 largely varied among biomes, with the largest increases in the tundra and boreal needleleaf deciduous forest. Compared to the low emission scenario (B1), the high emission scenario (A2) would lead to larger spatiotemporal variations in NPP, and more dramatic and counteracting impacts from climate and increasing atmospheric CO2.
Related JoVE Video
Solitary hepatic lymphangioma: a one-case report.
Springerplus
PUBLISHED: 01-01-2014
Show Abstract
Hide Abstract
Hepatic lymphangiomas, malformations of the liver lymphatic system, are extremely rare conditions in adults. A 41-year-old man presented with right upper abdominal pain for 6 months was introduced in this report. Ultrasound (US) and computed tomography (CT) scan demonstrated a giant cystictumor with a pedunculatedextrahepatic growth pattern. Due to diagnostic uncertainty, a partial hepatectomy was performed and pathological results confirmed the diagnosis of solitary hepatic lymphangioma. In this article, we reviewed the clinical and pathology features, preoperative diagnostic challenges, and treatments of hepaticlymphangiomas.
Related JoVE Video
Risk factors associated with early and late HAT after adult liver transplantation.
World J. Gastroenterol.
PUBLISHED: 01-01-2014
Show Abstract
Hide Abstract
To identify risk factors that might contribute to hepatic artery thrombosis (HAT) after liver transplantation (LT).
Related JoVE Video
Clinical and genetic characteristics for the Urofacial Syndrome (UFS).
Int J Clin Exp Pathol
PUBLISHED: 01-01-2014
Show Abstract
Hide Abstract
The Urofacial (Ochoa) Syndrome (UFS) is a rare autosomal recessive disorder and over 100 patients have been reported thus far. UFS is characterized by the abnormal facial expression and dysfunctional voiding. The patients show a peculiar distortion of the facial expression (grimacing as if in pain or sadness when they tried to smile or laugh) along with urinary tract infection, enuresis, vesicoureteral reflux and hydronephrosis without any underlying neurological lesion and previous urinary obstruction. Some patients are also noted with nocturnal lagophthalmos. Until 2010, HPSE2, the gene encodes Heparanse 2 on chromosome 10, was thought to be the only culprit gene for this syndrome. However, another criminal gene, LRIG2, which encodes leucine-rich repeats and immunoglobulin-like domains 2, was also come into the light in 2012. Studies for dissecting the biological functions of HPSE2 and LRIG2 in urinary abnormalities are ongoing. In this minireview, we will update the discovery of novel clinical manifestations relevant to this syndrome and discuss with focus for the impact of HPSE2 on voiding dysfunction.
Related JoVE Video
Climate and land use controls on soil organic carbon in the loess plateau region of China.
PLoS ONE
PUBLISHED: 01-01-2014
Show Abstract
Hide Abstract
The Loess Plateau of China has the highest soil erosion rate in the world where billion tons of soil is annually washed into Yellow River. In recent decades this region has experienced significant climate change and policy-driven land conversion. However, it has not yet been well investigated how these changes in climate and land use have affected soil organic carbon (SOC) storage on the Loess Plateau. By using the Dynamic Land Ecosystem Model (DLEM), we quantified the effects of climate and land use on SOC storage on the Loess Plateau in the context of multiple environmental factors during the period of 1961-2005. Our results show that SOC storage increased by 0.27 Pg C on the Loess Plateau as a result of multiple environmental factors during the study period. About 55% (0.14 Pg C) of the SOC increase was caused by land conversion from cropland to grassland/forest owing to the government efforts to reduce soil erosion and improve the ecological conditions in the region. Historical climate change reduced SOC by 0.05 Pg C (approximately 19% of the total change) primarily due to a significant climate warming and a slight reduction in precipitation. Our results imply that the implementation of "Grain for Green" policy may effectively enhance regional soil carbon storage and hence starve off further soil erosion on the Loess Plateau.
Related JoVE Video
[Pregnant rate and pregnancy-relating factors of patients with early endometrial carcinoma and severe atypical hyperplasia of endometrium after fertility-preserving treatment by progestin].
Zhonghua Fu Chan Ke Za Zhi
PUBLISHED: 11-29-2013
Show Abstract
Hide Abstract
To summarize the pregnant rate of patients with early endometrial carcinoma and severe atypical hyperplasia after fertility-preserving treatment and analyze their pregnancy-relating factors.
Related JoVE Video
Thermal property microscopy with frequency domain thermoreflectance.
Rev Sci Instrum
PUBLISHED: 11-05-2013
Show Abstract
Hide Abstract
A thermal property microscopy technique based on frequency domain thermoreflectance (FDTR) is presented. In FDTR, a periodically modulated laser locally heats a sample while a second probe beam monitors the surface reflectivity, which is related to the thermal properties of the sample with an analytical model. Here, we extend FDTR into an imaging technique capable of producing micrometer-scale maps of several thermophysical properties simultaneously. Thermal phase images are recorded at multiple frequencies chosen for maximum sensitivity to thermal properties of interest according to a thermal model of the sample. The phase versus frequency curves are then fit point-by-point to obtain quantitative thermal property images of various combinations of thermal properties in multilayer samples, including the in-plane and cross-plane thermal conductivities, heat capacity, thermal interface conductance, and film thickness. An FDTR microscope based on two continuous-wave lasers is described, and a sensitivity analysis of the technique to different thermal properties is carried out. As a demonstration, we image ~3 nm of patterned titanium under 100 nm of gold on a silicon substrate, and simultaneously create maps of the thermal interface conductance and substrate thermal conductivity. Results confirm the potential of our technique for imaging and quantifying thermal properties of buried layers, indicating its utility for mapping thermal properties in integrated circuits.
Related JoVE Video
Comparison of hepatitis B prophylactic outcomes in living donor liver transplantation recipients who meet the Milan or UCSF criteria.
HBPD INT
PUBLISHED: 10-10-2013
Show Abstract
Hide Abstract
The tumor burden before liver transplantation indicates that hepatitis B virus (HBV) may hide in the extrahepatic and micrometastatic sites which serve as a source of HBV replication. Currently, many liver transplant centers, especially in Western countries, use the Milan or UCSF criteria to select patients with hepatocellular carcinoma for liver transplantation. This study was undertaken to compare the HBV prophylactic outcomes in two groups of living donor liver transplantation (LDLT) recipients. Patients in group A met the Milan criteria and those in group B exceeded the Milan criteria but were within the UCSF criteria.
Related JoVE Video
Bile leakage test in liver resection: A systematic review and meta-analysis.
World J. Gastroenterol.
PUBLISHED: 08-18-2013
Show Abstract
Hide Abstract
To assess systematically the safety and efficacy of bile leakage test in liver resection.
Related JoVE Video
Analysis of 30 patients with persistent or recurrent squamous cell carcinoma of the cervix within one year after concurrent chemoradiotherapy.
Cancer Biol Med
PUBLISHED: 08-07-2013
Show Abstract
Hide Abstract
To investigate the recurrence sites, risk factors, and prognosis of patients with persistent or recurrent squamous cell carcinoma (SCC) of the cervix within one year after undergoing concurrent chemoradiotherapy (CCRT).
Related JoVE Video
Identification of differentially expressed long non-coding RNAs in human ovarian cancer cells with different metastatic potentials.
Cancer Biol Med
PUBLISHED: 08-02-2013
Show Abstract
Hide Abstract
To identify differentially expressed long non-coding RNAs (lncRNAs) involved in the metastasis of epithelial ovarian cancer.
Related JoVE Video
[Application of total hemihepatic vascular exclusion in liver resection for patients with hepatocellular carcinoma and impaired liver function].
Zhonghua Wai Ke Za Zhi
PUBLISHED: 07-31-2013
Show Abstract
Hide Abstract
To study the clinical value of total hemihepatic vascular exclusion (THHVE) in liver resection for patients with hepatocellular carcinoma (HCC) and impaired liver function.
Related JoVE Video
Down-regulation of synaptotagmin 1 in cortex, hippocampus, and cerebellum after experimental subarachnoid hemorrhage.
Ann. Clin. Lab. Sci.
PUBLISHED: 07-26-2013
Show Abstract
Hide Abstract
Synapses are formed by the coordinated assembly and tight attachment of pre- and postsynaptic specializations. Synaptotagmin 1 (Syt 1) has been proven to be an important factor for synapse function and behavioral cognition. The current research was aimed to investigate the expression of Syt 1 in the brain after an experimental subarachnoid hemorrhage (SAH) in rats. A total of 42 rats were randomly divided into seven groups: normal group; control group; day 1, day 3, day 5, day 7 and day 14 groups. Day 1, day 3, day 5, day 7, and day 14 groups were all SAH groups in which the rats were killed on day 1, 3, 5, 7, and 14, respectively. The rat SAH model was induced by injection of 0.3 ml of fresh arterial, non-heparinized blood into the prechiasmatic cistern for 20 sec. Immunostaining and immunoblotting were performed to detect the expression of the Syt 1 protein. The expressions of the Syt 1 protein decreased remarkably in SAH groups compared to the control group. The down-regulated expression of Syt 1 was detected after SAH and the low ebb was on days 1-3. The immunohistochemical staining demonstrated the expression of Syt 1 to be present mainly in the neurons of the cortex, hippocampus, and cerebellum. Our results indicated that Syt 1 expression is down-regulated in the brain after experimental SAH. These finding suggests that the decreased Syt 1 expression may facilitate the development of cognitive dysfunction after SAH.
Related JoVE Video
MSCs transplantation with application of G-CSF reduces apoptosis or increases VEGF in rabbit model of myocardial infarction.
Cytotechnology
PUBLISHED: 07-05-2013
Show Abstract
Hide Abstract
The purpose of this study was to test whether mesenchymal stem cells (MSCs) transplantation with application of granulocyte colony-stimulating factor (G-CSF) would have beneficial effects on damaged heart in a rabbit model of myocardial infarction (MI). MI was created by ligation of the left anterior descending coronary artery. After induction of MI, 40 New Zealand white rabbits were randomly divided into 8 groups: (1) MSCs injection at 3 days after MI; (2) G-CSF injection at 3 days after MI; (3) MSCs + G-CSF (20 u/kg/day) injection at 3 days after MI; (4) PBS injection at 3 days after MI; (5) MSCs injection at 7 days after MI; (6) G-CSF injection at 7 days after MI; (7) MSCs + G-CSF (20 u/kg/day) injection 7 days after MI; and (8) PBS injection 7 days after MI. TUNEL analysis showed that the apoptotic cells were distributed in the marginal area of MI. In both 3 and 7 days after MI groups, there were less apoptotic cells in the MSCs and MSCs + G-CSF groups as compared with the PBS group (P < 0.05). However, no decrease in apoptosis was observed in the G-CSF only group (P > 0.05). Immunohistochemistry analysis demonstrated that the expression level of vascular endothelial growth factor was higher in the MSCs, MSCs + G-CSF and G-CSF groups as compared with the PBS group. The present study demonstrated a beneficial effect of MSCs transplantation with application of G-CSF in the treatment of rabbit MI.
Related JoVE Video
[Outcome comparison of open versus endovascular techniques in the treatment of abdominal aortic aneurysms].
Zhonghua Yi Xue Za Zhi
PUBLISHED: 06-12-2013
Show Abstract
Hide Abstract
To compare the efficacy, cost and safety of endovascular aortic repair (EVAR) versus open surgery in the treatment of infrarenal abdominal aortic aneurysms.
Related JoVE Video
Estrogen inhibits colon polyp formation by reducing angiogenesis in a carcinogen-induced rat model.
Int J Endocrinol
PUBLISHED: 06-02-2013
Show Abstract
Hide Abstract
Objective. To study the effects of estrogen on colon polyp formation, proliferation, and angiogenesis on a rat model of colon cancer induced by dimethylhydrazine (DMH). Methods. Thirty-six female ovariectomized (OVX) rats were randomly divided into 3 groups: (I) control group (administrated with vehicles weekly), (II) DMH group (administrated with DMH weekly), and (III) DMH + E2 group (administrated with DMH and 17?-estradiol weekly). The incidence, volumes, and multiplicity of colon polyps in each group were evaluated. The microvessel density (MVD), the expressions of Proliferating Cell Nuclear Antigen (PCNA), and the expressions of HIF-1 ? and VEGF in polyps were detected in each group. Results. Estrogen reduced the multiplicity, volumes, and the PCNA expressions of DMH-induced colon polyps. The MVD in DMH + E2 group was significantly lower than that in DMH group. Estrogen treatment decreased the HIF-1 ? and VEGF expressions at both mRNA and protein level. Conclusion. Estrogen replacement was protective for ovariectomized rats from DMH-induced carcinogenesis, and one of the mechanisms for this was due to estrogens inhibitive effects on blood vessel formation by downregulating VEGF and HIF-1 ? expressions.
Related JoVE Video
Neutrophil-lymphocyte ratio predicts the prognosis of patients with hepatocellular carcinoma after liver transplantation.
World J. Gastroenterol.
PUBLISHED: 05-19-2013
Show Abstract
Hide Abstract
To determine whether an elevated neutrophil-lymphocyte ratio (NLR) is negatively associated with tumor recurrence in patients with hepatitis B virus (HBV)-related hepatocellular carcinoma (HCC) after liver transplantation (LT), and to determine the optimal predictive NLR cut-off value.
Related JoVE Video
Uncovering a key to the process of metastasis in human cancers: a review of critical regulators of anoikis.
J. Cancer Res. Clin. Oncol.
PUBLISHED: 05-14-2013
Show Abstract
Hide Abstract
Anoikis (homelessness in Greek) is a form of apoptosis following the detachment of cells from the appropriate extracellular matrix (Chiarugi and Giannoni in Biochem Pharmacol 76:1352-1364, 2008). Resistance to anoikis is a critical mediator of metastasis in cancer by enabling cancer cells to survive during invasion and transport in the blood and lymph. Numerous regulators and mechanisms of anoikis in human cancer have been proposed to date. Consequently, the identification of key regulators of anoikis that can be targeted to at least partially restore anoikis sensitivity in cancer cells is important in the development of therapies to treat metastatic cancer.
Related JoVE Video
[Effects of long-term intervention of moxa smoke on T lymphocyte subsets and CD4+ CD25+ Treg in peripheral blood of Wistar rats].
Zhongguo Zhen Jiu
PUBLISHED: 04-30-2013
Show Abstract
Hide Abstract
To investigate the cellular immune regulation of the long-term intervention of moxa smoke.
Related JoVE Video
[Effect of borate polymer layers on measurement of glucose concentration by SPR].
Guang Pu Xue Yu Guang Pu Fen Xi
PUBLISHED: 04-17-2013
Show Abstract
Hide Abstract
A new borate polymer PAA-ran-PAAPBA that can specifically adsorb glucose was introduced in the glucose measurement based on surface plasmon resonance, and the high-precision specific detection of glucose concentration was realized. Six layers and twelve layers of borate polymer were respectively bound onto the SPR sensors through the layer-by-layer self-assembly binding method, and the effect of different layers of borate polymer on the glucose surface plasmon resonance measurement was studied. The experiment was conducted in the concentration range of 1-10 mg x dL(-1) (interval delta = 1 mg x dL(-1)), 10-100 mg x dL(-1) (interval delta = 10 mg x dL(-1)), and 100-1 000 mg x dL(-1) (interval delta = 100 mg x dL(-1)), experiment data was fitted by quadric curve and the fitting degree of refractive index difference deltaRU and glucose concentration was obtained. Results showed that the 12-layer-polymer sensor was better than the 6-layer-polymer sensor in the first two smaller ranges, and the measuring result was not significantly affected by layers in the third range, indicating that for the small concentrations increasing polymer layer can dramatically improve the measurement.
Related JoVE Video
Congenital Perineal Mass with Cervovaginal Duplication: A Case Report and Literature Review.
J Pediatr Adolesc Gynecol
PUBLISHED: 04-14-2013
Show Abstract
Hide Abstract
Congenital perineal mass is rare. Although there have been several reports describing male neonates with a perineal mass with urogenital anomalies, no similar cases have been reported in female infants.
Related JoVE Video
11 kGy gamma irradiated demineralized bone matrix enhances osteoclast activity.
Eur J Orthop Surg Traumatol
PUBLISHED: 04-10-2013
Show Abstract
Hide Abstract
BACKGROUND: Demineralized bone matrix (DBM) allografts are widely used in orthopaedic clinics. However, the biological impact on its osteoinductivity after its sterilization process by gamma irradiation is not well studied. Furthermore, little is known about the relationship between residual calcium levels on osteoinductivity. HYPOTHESIS: We hypothesize that low-dose gamma irradiation retains the osteoinducitivity properties of DBM and causes ectopic bone formation. MATERIALS AND METHODS: A randomised animal trial was performed to compare tissue and molecular responses of low-dose (11 kGy) gamma irradiated and non-irradiated human DBM at 6 weeks post-intramuscular implantation using an athymic rat model. In addition, we correlated residual calcium levels and bone formation in gamma irradiated DBM. RESULTS: A modified haematoxylin and eosin stain identified ectopic bony capsules at all implanted sites with no significant difference on the amount of new bone formed between the groups. Statistically significantly lower ratio of alkaline phosphatase expression over tartrate-resistant acid phosphatase and/or cathepsin K expressions was found between the groups. DISCUSSION: This study found that low-dose gamma irradiated DBM, which provides a sterility assurance level of 10(-6) for bone allografts, retained osteoinductivity but exhibited significantly enhanced osteoclastic activity. Furthermore, this is the first study to find a positive correlation between residual calcium levels and bone formation in gamma irradiated DBM.
Related JoVE Video
Value of ?-fetoprotein in association with clinicopathological features of hepatocellular carcinoma.
World J. Gastroenterol.
PUBLISHED: 04-05-2013
Show Abstract
Hide Abstract
To explore the relationship between ?-fetoprotein (AFP) and various clinicopathological variables and different staging system of hepatocellular carcinoma (HCC) thoroughly.
Related JoVE Video
Relevant markers of cancer stem cells indicate a poor prognosis in hepatocellular carcinoma patients: a meta-analysis.
Eur J Gastroenterol Hepatol
PUBLISHED: 03-13-2013
Show Abstract
Hide Abstract
The potential prognostic value of stem cell markers is variably reported, such as CD133 and epithelial cell adhesion molecule. However, their clinical value and significance in hepatocellular carcinoma (HCC) remain controversial. The aim of this study was to carry out a meta-analysis of literature evaluating CD133 and epithelial cell adhesion molecule expression as prognostic factors in HCC and to determine the association between cancer stem cells (CSCs) and common clinical and pathologic features of HCC. The relevant literature was identified using Science Direct, EMBASE, and PubMed. Outcome measures included disease-free survival, overall survival, and relevant pathological parameters. Meta-analyses were carried out using Review Manager, version 5.2. Twelve eligible articles involving 1344 patients were included. Meta-analyses showed that the presence of CSCs was significantly associated with a poor histological grade (OR=3.16, P=0.003) and elevated serum ?-fetoprotein level (OR=2.68, P<0.00001). However, there were no significant relations between the presence of CSCs and tumor size, tumor stage, hepatitis, or cirrhosis. The presence of CSCs was significantly associated with poor survival, including overall survival (HR=1.62, P<0.00001) and disease-free survival (HR=1.85, P<0.00001). On the basis of current retrospective evidence, the presence of CSCs is associated with poor histopathologic grade and worse survival in patients with HCC and CD133 plays a significant role in predicting the clinical outcome. Further extensive experimental and clinical research should be carried out to evaluate the role of these markers in clinical practice.
Related JoVE Video
Anti-inflammatory effects of propofol are mediated by apolipoprotein M in a hepatocyte nuclear factor-1?-dependent manner.
Arch. Biochem. Biophys.
PUBLISHED: 03-01-2013
Show Abstract
Hide Abstract
Propofol (2,6-diisopropylphenol) is probably the most widely used intravenous hypnotic agent in daily practice. However, its anti-inflammatory properties have seldom been addressed. In this study, we evaluated the anti-inflammatory activity and mechanisms of propofol on lipopolysaccharide (LPS)-induced inflammation in vivo and in vitro and found that propofol markedly inhibited LPS-induced production of pro-inflammatory cytokines, including tumor necrosis factor (TNF)-?, interleukin (IL)-1?, and IL-6, and expression of inducible nitric oxide synthase (iNOS). At the same time, the expression of hepatocyte nuclear factor-1? (HNF-1?) and apolipoprotein M (APOM) was inhibited by treatment with LPS and LPS-induced down-regulation of HNF-1? expression and APOM expression could be compensated by propofol treatment. However, propofol could not compensate LPS-induced down-regulation of APOM expression by treatment with HNF-1? siRNA and the suppressive effect on LPS-induced pro-inflammatory cytokines production by propofol was significantly compensated by treatment with APOM siRNA. These results provide evidence that propofol may first up-regulate APOM expression by enhancing HNF-1? expression and then inhibit pro-inflammatory cytokine production in LPS-stimulated cells. Therefore, our study may be useful in understanding the critical effect of propofol in patients with systemic inflammatory response syndrome.
Related JoVE Video
Impaired translocation and activation of mitochondrial Akt1 mitigated mitochondrial oxidative phosphorylation Complex V activity in diabetic myocardium.
J. Mol. Cell. Cardiol.
PUBLISHED: 02-26-2013
Show Abstract
Hide Abstract
Insulin can translocate Akt to mitochondria in cardiac muscle. The goals of this study were to define sub-mitochondrial localization of the translocated Akt, to dissect the effects of insulin on Akt isoform translocation, and to determine the direct effect of mitochondrial Akt activation on Complex V activity in normal and diabetic myocardium. The translocated Akt sequentially localized to the mitochondrial intermembrane space, inner membrane, and matrix. To confirm Akt translocation, in vitro import assay showed rapid entry of Akt into mitochondria. Akt isoforms were differentially regulated by insulin stimulation, only Akt1 translocated into mitochondria. In the insulin-resistant Type 2 diabetes model, Akt1 translocation was blunted. Mitochondrial activation of Akt1 increased Complex V activity by 24% in normal myocardium in vivo and restored Complex V activity in diabetic myocardium. Basal mitochondrial Complex V activity was lower by 22% in the Akt1(-/-) myocardium. Insulin-stimulated Complex V activity was not impaired in the Akt1(-/-) myocardium, due to compensatory translocation of Akt2 to mitochondria. Akt1 is the primary isoform that relayed insulin signaling to mitochondria and modulated mitochondrial Complex V activity. Activation of mitochondrial Akt1 enhanced ATP production and increased phosphocreatine in cardiac muscle cells. Dysregulation of this signal pathway might impair mitochondrial bioenergetics in diabetic myocardium.
Related JoVE Video
A new electrochemically active bacterium phylogenetically related to Tolumonas osonensis and power performance in MFCs.
Bioresour. Technol.
PUBLISHED: 02-13-2013
Show Abstract
Hide Abstract
A facultative anaerobic bacterium (designated as P2-A-1) was isolated from microbial fuel cells (MFCs) inoculated with sludge from a sewage treatment plant. Based on 16S rDNA sequence analysis, the strain was identified as Tolumonas osonensis OCF 7(T) according to its biochemical, physiological and morphological characteristics. Through parameters optimization, the P2-A-1 MFC reached the maximum power density of 424 mW/m(2) in the substrate of 2g/L sodium acetate. Further, a facile bacteria treatment approach by chemically "perforating" pores and channels on bacterial membrane was developed to significantly improve the power density. And 1mM of EDTA-treated cell yielded the highest power density of 509.1 mW/m(2) because the membrane permeability of cell was enhanced by verification of coenzyme Q and fatty acid composition tests. It offers a novel facultative anaerobic Gram-positive bacterium that can utilize a wide variety of substrates for power production, making it highly valuable for application in MFCs.
Related JoVE Video
Comparison of Iodine-125 seeds implantation and afterloading radiotherapy in murine models of human cervical cancer.
Arch. Gynecol. Obstet.
PUBLISHED: 01-08-2013
Show Abstract
Hide Abstract
To compare the efficacies and side effects of Iodine-125 ((125)I) seeds implantation with afterloading radiotherapy on tumor xenografts derived from Hela cells.
Related JoVE Video
Scoring selection criteria including total tumour volume and pretransplant percentage of lymphocytes to predict recurrence of hepatocellular carcinoma after liver transplantation.
PLoS ONE
PUBLISHED: 01-01-2013
Show Abstract
Hide Abstract
The selection criteria for patients with hepatocellular carcinoma (HCC) to undergo liver transplantation should accurately predict posttransplant recurrence while not denying potential beneficiaries. In the present study, we attempted to identify risk factors associated with posttransplant recurrence and to expand the selection criteria.
Related JoVE Video
Skeletal muscle mitochondrial depletion and dysfunction in chronic kidney disease.
Int J Clin Exp Med
PUBLISHED: 01-01-2013
Show Abstract
Hide Abstract
Advanced chronic kidney disease (CKD) is associated with impaired exercise capacity, skeletal muscle dysfunction, and oxidative stress. Mitochondria are the primary source for energy production and generation of reactive oxygen species (ROS). Mitochondrial state 3 respiration, mitochondrial complex I enzyme activity, and tissue porin/actin ratio were determined in the gastrocnemius muscle of male SD rats 14 weeks after 5/6 nephrectomy (CKD) or sham-operation (control). The CKD group exhibited azotemia, hypertension, significant reduction (-39%) of state 3 mitochondrial respiration, and a significant increase in the mitochondrial complex I enzyme activity. The latter is the first step in oxidative phosphorylation, a process linked to production of ROS. These abnormalities were associated with a significant reduction in muscle porin/? actin ratio denoting substantial reduction of mitochondrial mass in skeletal muscle of animals with CKD. CKD results in impaired mitochondrial respiration, reduced muscle mitochondrial mass, depressed energy production and increased ROS generation in the skeletal muscle. These events can simultaneously contribute to the reduction of exercise capacity and oxidative stress in CKD.
Related JoVE Video
Dihydrocapsaicin Attenuates Plaque Formation through a PPAR?/LXR? Pathway in apoE(-/-) Mice Fed a High-Fat/High-Cholesterol Diet.
PLoS ONE
PUBLISHED: 01-01-2013
Show Abstract
Hide Abstract
Atherosclerosis is a chronic inflammatory disease and represents the major cause of cardiovascular morbidity and mortality. There is evidence that dihydrocapsaicin (DHC) can exert multiple pharmacological and physiological effects. Here, we explored the effect of DHC in atherosclerotic plaque progression in apoE(-/-) mice fed a high-fat/high-cholesterol diet.
Related JoVE Video
Mitochondrial Akt-regulated mitochondrial apoptosis signaling in cardiac muscle cells.
Am. J. Physiol. Heart Circ. Physiol.
PUBLISHED: 11-11-2011
Show Abstract
Hide Abstract
We recently reported translocation and activation of Akt in cardiac mitochondria. This study was to determine whether activation of Akt in mitochondria could inhibit apoptosis of cardiac muscle cells. Insulin stimulation induced translocation of phosphorylated Akt to the mitochondria in primary cardiomyocytes. A mitochondria-targeted constitutively active Akt was overexpressed via adenoviral vector and inhibited efflux of cytochrome c and apoptosis-inducing factor from mitochondria to cytosol and partially prevented loss of mitochondria cross-membrane electrochemical gradient. Activation of caspase 3 was suppressed in the cardiomyocytes transduced with mitochondria-targeted active Akt, whereas a mitochondria-targeted dominant negative Akt enhanced activation of caspase 3. Terminal deoxynucleotidyl transferase dUTP-mediated nick-end labeling assay showed that mitochondrial activation of Akt significantly reduced the number of apoptotic cells. When the endogenous Akt was abolished by LY294002, the antiapoptotic actions of mitochondrial Akt remained effective. These experiments suggested that mitochondrial Akt suppressed apoptosis signaling independent of cytosolic Akt in cardiac muscle cells.
Related JoVE Video

What is Visualize?

JoVE Visualize is a tool created to match the last 5 years of PubMed publications to methods in JoVE's video library.

How does it work?

We use abstracts found on PubMed and match them to JoVE videos to create a list of 10 to 30 related methods videos.

Video X seems to be unrelated to Abstract Y...

In developing our video relationships, we compare around 5 million PubMed articles to our library of over 4,500 methods videos. In some cases the language used in the PubMed abstracts makes matching that content to a JoVE video difficult. In other cases, there happens not to be any content in our video library that is relevant to the topic of a given abstract. In these cases, our algorithms are trying their best to display videos with relevant content, which can sometimes result in matched videos with only a slight relation.