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Find video protocols related to scientific articles indexed in Pubmed.
Liquid Crystalline Behavior of Graphene Oxide in the Formation and Deformation of Tough Nanocomposite Hydrogels.
Langmuir
PUBLISHED: 11-18-2014
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In this article we report the formation and transformation of graphene oxide (GO) liquid crystalline (LC) structures in the synthesis and deformation of tough GO nanocomposite hydrogels. GO aqueous dispersions form nematic LC phase, while the addition of poly(N-vinylpyrrolidone) (PVP) and acrylamide (AAm), which are capable of forming hydrogen bonding with GO nanosheets, shifts the isotropic/nematic transition to a lower volume fraction of GO and enhance the formation of nematic droplets. During the gelation process, a phase separation of the polymers and GO nanosheets is accompanied by the directional assembly of GO nanosheets, forming large LC tactoids with a radial GO configuration. The shape of the large tactoids evolves from a sphere to a toroid as the tactoids increase in size. Interestingly, during cyclic uniaxial tensile deformation a reversible LC transition is observed in the very tough hydrogels. The isolated birefringent domains and the LC domains in the tactoids in the gels are highly oriented under a high tensile strain.
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Novel NTRK1 Frameshift Mutation in Congenital Insensitivity to Pain With Anhidrosis.
J. Child Neurol.
PUBLISHED: 10-16-2014
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Congenital insensitivity to pain with anhidrosis is a rare autosomal recessive disorder. It has been reported that the defect in the NTRK1 gene encoding tropomyosin-related kinase A (TrkA) can cause congenital insensitivity to pain with anhidrosis. Nerve growth factor (NGF), the product of NGFB, mediates biological effects by binding to and activating tropomyosin-related kinase A. In addition, necdin (encoded by NDN) is also essential in nerve growth factor-tropomyosin-related kinase A pathway. We performed mutation analysis in NTRK1, NGFB, and NDN genes in a Chinese Han 17-year-old female patient with congenital insensitivity to pain with anhidrosis and her healthy family members. As a result, the patient was found to have a novel insertion in exon 7 (c.727insT) of NTRK1, which causes premature termination, and a single nucleotide polymorphism (rs2192206 G>A) in NDN. Our findings imply that the genetic variations of the nerve growth factor-tropomyosin-related kinase A pathway play an important role in congenital insensitivity to pain with anhidrosis.
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Effects of integrin ???3 on differentiation and collagen synthesis induced by connective tissue growth factor in human hypertrophic scar fibroblasts.
Int. J. Mol. Med.
PUBLISHED: 08-22-2014
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CCN2 is a matricellular protein that appears to be important in scar formation. CCN2 mediates the pro-fibrotic effects in hypertrophic scars (HTSs) through an unknown mechanism. However, many activities of CCN2 protein are known to be mediated by direct binding to integrin receptors. In this study, we investigated the role of integrin ?(?)?(3) in the differentiation of hypertrophic scar fibroblasts (HTSFs) induced by CCN2. The levels of integrin ?(?)?(3) between normal skin and hypertrophic scar (HTS) tissues were compared, and integrin ?(?)?(3) was found to be upregulated in HTS. CCN2 was shown to induce HTSF differentiation and collagen (COL) synthesis at the mRNA and protein levels. Based on these results, the expression of integrin ?(?)?(3) was upregulated by CCN2 stimulation during HTSF differentiation. Blockade of integrin ?(?)?(3) prevented CCN2-induced HTSF differentiation and COL synthesis. Furthermore, the CCN2-induced increase in contractility of the HTSF in COL lattices was inhibited by integrin ?(?)?(3) blocking antibodies. HTSs were established in a rabbit ear model, and the inhibitor of integrin ?(?)?(3) signi?cantly improved the architecture of the rabbit ear scar. Results of the present study showed that integrin ?(?)?(3) contributes to pro-fibrotic CCN2 signaling. Blocking this pathway may therefore be beneficial for the treatment of HTS.
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Association of LMX1A genetic polymorphisms with susceptibility to congenital scoliosis in Chinese Han population.
Spine
PUBLISHED: 08-08-2014
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A genetic association study of single nucleotide polymorphisms (SNPs) for the LMX1A gene with congenital scoliosis (CS) in the Chinese Han population.
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[Comparative analysis of decitabine combined with DAG regimen and other regimens in treatment of refractory/relapsed acute myeloid leukemia].
Zhonghua Xue Ye Xue Za Zhi
PUBLISHED: 07-03-2014
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To compare the clinical efficacy and safety among different chemotherapeutic regimens in treatment of refractory/relapsed acute myeloid leukemia (AML).
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Comparative toxicity of new halophenolic DBPs in chlorinated saline wastewater effluents against a marine alga: halophenolic DBPs are generally more toxic than haloaliphatic ones.
Water Res.
PUBLISHED: 06-01-2014
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Using seawater for toilet flushing effectively reduces the consumption of precious freshwater resources, yet it introduces bromide and iodide ions into a wastewater treatment system, which may form bromo- and iodo-disinfection byproducts (DBPs) during chlorination of the wastewater effluent. Most of the newly identified DBPs in chlorinated wastewater effluents were halophenolic compounds. It has been reported that the newly identified bromo- and iodo-phenolic DBPs were generally significantly more toxic to a heterotrophic marine polychaete than the commonly known haloacetic acids and trihalomethanes. This has raised a concern over the discharge of chlorinated saline wastewater effluents into the marine ecosystem. In this study, the toxicity of new halophenolic DBPs and some haloaliphatic DBPs was tested against an autotrophic marine alga, Tetraselmis marina. The alga and polychaete bioassays gave the same toxicity orders for many groups of halo-DBPs. New halophenolic DBPs also showed significantly higher toxicity to the alga than the commonly known haloacetic acids, indicating that the emerging halophenolic DBPs deserve more attention. However, two bioassays did exhibit a couple of disparities in toxicity results, mainly because the alga was capable of metabolizing some (nitrogenous) halophenolic DBPs. A quantitative structure-toxicity relationship was developed for the halophenolic DBPs, by employing three physicochemical descriptors (log K(ow), pKa and molar topological index). This relationship presented the toxicity mechanism of the halophenolic DBPs to T. marina and gave a good prediction of the algal toxicity of the tested halophenolic DBPs.
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The effectiveness and safety of avanafil for erectile dysfunction: a systematic review and meta-analysis.
Curr Med Res Opin
PUBLISHED: 04-14-2014
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To compare the efficacy and safety between different dosages of avanafil for the treatment of erectile dysfunction (ED).
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Monitoring collagen synthesis in fibroblasts using fluorescently labeled tRNA pairs.
J. Cell. Physiol.
PUBLISHED: 03-20-2014
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There is a critical need for techniques that directly monitor protein synthesis within cells isolated from normal and diseased tissue. Fibrotic disease, for which there is no drug treatment, is characterized by the overexpression of collagens. Here, we use a bioinformatics approach to identify a pair of glycine and proline isoacceptor tRNAs as being specific for the decoding of collagen mRNAs, leading to development of a FRET-based approach, dicodon monitoring of protein synthesis (DiCoMPS), that directly monitors the synthesis of collagen. DiCoMPS aimed at detecting collagen synthesis will be helpful in identifying novel anti-fibrotic compounds in cells derived from patients with fibrosis of any etiology, and, suitably adapted, should be widely applicable in monitoring the synthesis of other proteins in cells.
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Excessive pulse pressure response to standing in community population with orthostatic systolic hypertension.
J Am Soc Hypertens
PUBLISHED: 03-18-2014
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The postural change of pulse pressure (PP) in the persons with orthostatic hypertension (OHT) is unclear. This study included 2849 (65.0 ± 9.3 years) community participants. Blood pressures (BPs) in supine and standing positions were measured. The differences between upright and supine BP and PP were recorded as ?BP and ?PP. The criteria for OHT was ?BP ?10 mm Hg, for orthostatic hypotension (OH) was ?-10 mm Hg and for orthostatic normotension (ONT) was -9 to 9 mm Hg. Fasting blood lipids and glucose were measured. The supine SBP of the sOHT group were similar to that of sONT group (140.9 ± 20.2 mm Hg vs 138.2 ± 19.7 mm Hg), but significantly lower than that of sOH group (151.9 ± 19.2 mm Hg; P < .05). Their PPs were 65.3 ± 15.9, 62.8 ± 14.7, and 71.1 ± 15.1 mm Hg, respectively, and with the similar group difference like SBP. When the position changed from supine to standing, the sOHT group showed PP rise, while sOH and sONT groups showed PP reduction (3.8 ± 7.1 mm Hg vs -17.0 ± 8.5 mm Hg and -5.8 ± 6.6 mm Hg; both P < .05). Thus, the standing PP in the sOHT group was significantly higher than in the sONT (69.1 ± 18.0 mm Hg vs 57.0 ± 15.8 mm Hg; P < .05) and in the sOH (54.2 ± 15.2 mm Hg; P < .05) groups. The postural PP profile varies with the postural responses of SBP. The sOHT group has obviously increased PP and significantly higher standing PP compared with the sONT group.
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Formation of brominated disinfection byproducts during Chloramination of drinking water: new polar species and overall kinetics.
Environ. Sci. Technol.
PUBLISHED: 02-19-2014
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The formation of brominated disinfection byproducts (Br-DBPs), which are generally significantly more cytotoxic and genotoxic than their chlorinated analogues, in chloramination has not been fully examined. In this work, the formation of new polar Br-DBPs in simulated drinking waters was examined using state-of-the-art ultraperformance liquid chromatography/electrospray ionization-triple quadrupole mass spectrometry. As many as 29 aliphatic, aromatic, or nitrogenous polar Br-DBPs were detected in chloramination, and five of them (including 2,4,6-tribromoresorcinol, 2,6-dibromo-4-nitrophenol, 2,2,4-tribromo-5-hydroxy-4-cyclopentene-1,3-dione, 2,2,4-dibromochloro-5-hydroxy-4-cyclopentene-1,3-dione, and 2,2,4-bromodichloro-5-hydroxy-4-cyclopentene-1,3-dione) were tentatively identified. Unlike chlorination, chloramination favored the formation of aromatic and nitrogenous polar Br-DBPs and was mild enough to allow polar intermediate Br-DBPs to accumulate. To further explore the formation mechanism of Br-DBPs in chloramination, a quantitative empirical model involving 33 major reactions was developed to describe the overall kinetics. According to the modeling results, bromochloramine and monobromamine were the major species responsible for 54.2-58.1% and 41.7-45.7%, respectively, of the formed Br-DBPs, while hypobromous acid accounted for only 0.2% of the formed Br-DBPs; direct reactions between monochloramine and natural organic matter accounted for the majority of the formed chlorinated DBPs (93.7-95.1%); hypochlorous acid and hypobromous acid in the chloramination were at ng/L or subng/L levels, which were not enough to cause polar intermediate Br-DBPs to decompose.
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CD146 as a new marker for an increased chondroprogenitor cell sub-population in the later stages of osteoarthritis.
J. Orthop. Res.
PUBLISHED: 02-17-2014
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Cartilage-derived mesenchymal stem cells (MSCs) have been isolated with different methods. In this study lateral and medial femoral condyles were respectively collected from patients with late-stage osteoarthritis during the total knee arthroplasty. After digestion of the cartilage tissues with type II collagenase and analysis by fluorescence-activated cell sorting (FACS) with CD146, a chondroprogenitor cell sub-population were isolated and purified. The expression of other MSC-associated markers in the CD146(+) chondroprogenitors was analyzed by flow cytometry. Multi-lineage differentiation capacity of CD146(+) chondroprogenitors was compared with that of unsorted chondrocytes and adipose-derived MSCs (ADMSCs). Higher percentage of CD146(+) chondroprogenitors isolated from the medial femoral condyles was observed than that from the lateral. CD146(+) chondroprogenitors expressed high levels of MSC-specific surface antigens, and showed higher chondrogenesis capacity than ADMSCs and unsorted chondrocytes in a 3D cell pellet culture model. Thus CD146 might be a new cell surface marker for cartilage progenitor cell population in the late-stage osteoarthritis. © 2014 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res.
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The involvement of ADAMTS-5 genetic polymorphisms in predisposition and diffusion tensor imaging alterations of lumbar disc degeneration.
J. Orthop. Res.
PUBLISHED: 01-10-2014
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Low back pain is a global health problem in which more than 40% is caused by lumbar intervertebral disc degeneration (LDD). ADAMTS-5 (A disintegrin and metalloproteinase with thrombospondin motifs-5) was shown to be involved in LDD by functional analyses. To identify whether there is an association between ADAMTS-5 and LDD, and what is the contribution of ADAMTS-5 genetic polymorphisms to MD (Mean diffusivity) changes in lumbar IVD (Intervertebral disc). We firstly genotyped selected ADAMTS-5 SNPs (Single nucleotide polymorphisms) in a Chinese Han population. After the primary analyses of allelic, genotypic, and haplotypic association, we performed SNP-SNP interaction analysis. We subsequently genotyped another 50 participants and acquired the corresponding MD values from individual lumbar IVDs. The association analysis between the genotypic groups divided by the above positive SNPs and the corresponding MD values were also performed. Significant associations were identified in rs151058, rs229052, and rs162502. None of the 2-SNP haplotypic analysis survived the 10,000 permutation test. The following interaction analysis demonstrated that rs151058 was strong associated with LDD when conditioning on rs162502. Significant difference of MD values between AA and G+ carriers was identified in rs162502. This is the first study indicating that the SNPs of ADAMTS-5 may contribute to predisposition of LDD. An interaction between rs151058 and rs229052 may exist in ADAMTS-5 with LDD. The rs162502 might be associated with altered MD values.
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Cellular and Molecular Mechanisms in Vascular Smooth Muscle Cells by which Total Saponin Extracted from Tribulus Terrestris Protects Against Artherosclerosis.
Cell. Physiol. Biochem.
PUBLISHED: 10-14-2013
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Background/Aims: Total saponin extracted from Tribulus terrestris (TSETT) has been reported to protect against atherosclerosis. We here investigate the cellular and molecular mechanisms of TSETT underlying protection against atherosclerosis. Methods: Cell proliferation was measured with Methyl thiazolyl tetrazolium (MTT); Intracellular H2O2 was measured with DCFH-DA, a fluorescent dye; Intracellular free Ca(2+) was measured with a confocal laser scanning microscopy; Genes expression was measured with gene array and real-time quantitative polymerase chain reaction (RT-PCR); Phosphorylation of extracellular signal-regulated kinase 1/2 (phospho-ERK1/2) was measured with cell-based enzyme-linked immunosorbent assay (ELISA) and western blotting. Results: TSETT significantly suppressed the increase in cells proliferation induced by angiotensin II, significantly suppressed the increase in the intracellular production of H2O2 induced by angiotensin II, significantly inhibited the increase in intracellular free Ca(2+) induced by H2O2, significantly inhibited the increase in phospho-ERK1/2 induced by angiotensin II; significantly inhibited the increase in mRNA expression of c-fos, c-jun and pkc-? induced by angiotensin II. Conclusion: These findings provide a new insight into the antiatherosclerotic properties of TSETT and provide a pharmacological basis for the clinical application of TSETT in anti-atherosclerosis. © 2013 S. Karger AG, Basel.
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A gut microbiota-targeted dietary intervention for amelioration of chronic inflammation underlying metabolic syndrome.
FEMS Microbiol. Ecol.
PUBLISHED: 06-04-2013
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Chronic inflammation induced by endotoxin from a dysbiotic gut microbiota contributes to the development of obesity-related metabolic disorders. Modification of gut microbiota by a diet to balance its composition becomes a promising strategy to help manage obesity. A dietary scheme based on whole grains, traditional Chinese medicinal foods, and prebiotics (WTP diet) was designed to meet human nutritional needs as well as balance the gut microbiota. Ninety-three of 123 central obese volunteers (BMI ? 28 kg m(-2) ) completed a self-controlled clinical trial consisting of 9-week intervention on WTP diet followed by a 14-week maintenance period. The average weight loss reached 5.79 ± 4.64 kg (6.62 ± 4.94%), in addition to improvement in insulin sensitivity, lipid profiles, and blood pressure. Pyrosequencing of fecal samples showed that phylotypes related to endotoxin-producing opportunistic pathogens of Enterobacteriaceae and Desulfovibrionaceae were reduced significantly, while those related to gut barrier-protecting bacteria of Bifidobacteriaceae increased. Gut permeability, measured as lactulose/mannitol ratio, was decreased compared with the baseline. Plasma endotoxin load as lipopolysaccharide-binding protein was also significantly reduced, with concomitant decrease in tumor necrosis factor-?, interleukin-6, and an increase in adiponectin. These results suggest that modulation of the gut microbiota via dietary intervention may enhance the intestinal barrier integrity, reduce circulating antigen load, and ultimately ameliorate the inflammation and metabolic phenotypes.
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Systemic inflammatory responses and multiple organ dysfunction syndrome following skin burn wound and Pseudomonas aeruginosa infection in mice.
Shock
PUBLISHED: 05-28-2013
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Burn wound-related sepsis is associated with the development of systemic inflammatory response syndrome and multiple organ dysfunction syndrome (MODS). This study is aimed at investigating the development and progression of SIS and MODS in a mouse model of skin burn sepsis. C57BL/6J mice were randomly divided into the sham, burn, Pseudomonas, and burn/Pseudomonas groups. The back skin of the sham, burn, and burn/Pseudomonas groups was burned about 10% of total area with using 37°C or 98°C water for 8 s, respectively, followed by inoculating with Pseudomonas aeruginosa. The Pseudomonas group was infected with P. aeruginosa without burn injury. Their body weights, mortality, organ histology, and function as well as systemic inflammation were measured longitudinally. The burn/Pseudomonas mice lost more body weights than did mice from the other groups and had a significantly higher mortality rate (P < 0.05). The burn/Pseudomonas mice exhibited significantly higher levels of bacterial loads in different organs and serum endotoxin, interleukin 1?, interleukin 6, tumor necrosis factor ?, and C-reactive protein than those in mice from the other groups (P < 0.05). The burn/Pseudomonas mice also displayed more severe liver, lung, and kidney tissue damage and impaired organ functions, particularly at 72 h after inoculation than did the burn and Pseudomonas groups of mice. Our data indicate that burn and P. aeruginosa infection induced severe sepsis and rapidly progressed into systemic inflammatory response syndrome and MODS in mice.
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Self-healing in tough graphene oxide composite hydrogels.
Macromol Rapid Commun
PUBLISHED: 05-08-2013
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Polymer hydrogels that are capable of spontaneously healing injury are being developed at a rapid pace because of their great potential in biomedical applications. Here, the self-healing property of tough graphene nanocomposite hydrogels fabricated by using graphene peroxide as polyfunctional initiating and cross-linking centers is reported. The hydrogels show excellent self-healing ability at ambient temperature or even lower temperatures for a short time and very high recovery degrees (up to 88% tensile strength) can be achieved at a prolonged healing time. The healed gels exhibit very high tensile strengths (up to 0.35 MPa) and extremely high elongations (up to 4900%). The strong interactions between the polyacrylamide chains and the graphene oxide sheets are essential to the mechanical strengths of the healed gels.
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Inhibition of Na+/H+ exchanger 1 by cariporide alleviates burn-induced multiple organ injury.
J. Surg. Res.
PUBLISHED: 04-18-2013
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Severe burns initiate an inflammatory response characterized by the upregulation of proinflammatory cytokine, which contributes to multiple organ injury. Na(+)/H(+) exchanger 1 (NHE1) plays a significant role in several inflammatory processes. This study was designed to investigate the role of NHE1 in burn-induced inflammation and multiple organ injury.
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The role of ERK and JNK signaling in connective tissue growth factor induced extracellular matrix protein production and scar formation.
Arch. Dermatol. Res.
PUBLISHED: 03-01-2013
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CCN2 plays an important role in the pathogenesis of hypertrophic scars (HTSs). Although CCN2 is involved in many fibroproliferative events, the CCN2 induction signaling pathway in HTSs is yet to be elucidated. Here, we first investigated the effect of the mitogen-activated protein kinases (MAPKs) on CCN2-induced ?-smooth muscle actin (?-SMA) and collagen I expression in human HTS fibroblasts (HTSFs). Then, we established HTSs in a rabbit ear model and determined the effect of MAPKs on the pathogenesis of HTSs. MAPK pathways were activated by CCN2 in HTSFs. Extracellular signal-regulated kinase (ERK) and c-Jun N-terminal kinase (JNK) inhibitors significantly inhibited CCN2-induced expression of ?-SMA and collagen I in HTSFs. In the rabbit ear model of the HTS, JNK and ERK inhibitors significantly improved the architecture of the rabbit ear scar and reduced scar formation on the rabbit ear. Our results indicate that ERK and JNK mediate collagen I expression and scarring of the rabbit ear, and may be considered for specific drug therapy targets for HTSs.
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Comparison of apparent diffusion coefficient and T2 relaxation time variation patterns in assessment of age and disc level related intervertebral disc changes.
PLoS ONE
PUBLISHED: 01-01-2013
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To compare the variation patterns of ADC and T2 values in different age and intervertebral disc (IVD) levels, thus to identify their sensitivities in assessing age and disc level related IVDs changes.
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Penetration of polar brominated DBPs through the activated carbon columns during total organic bromine analysis.
J Environ Monit
PUBLISHED: 08-22-2011
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Total organic bromine (TOBr) is a collective parameter representing all the brominated organic disinfection byproducts (DBPs) in water samples. TOBr can be measured using the adsorption-pyrolysis method according to Standard Method 5320B. This method involves that brominated organic DBPs are separated from inorganic halides and concentrated from aqueous solution by adsorption onto the activated carbon (AC). Previous studies have reported that some commonly known brominated DBPs can partially penetrate through the AC during this adsorption step. In this work, the penetration of polar brominated DBPs through AC and ozone-modified AC was explored with two simulated drinking water samples and one chlorinated wastewater effluent sample. Polar brominated DBPs were selectively detected with a novel precursor ion scan method using electrospray ionization-triple quadrupole mass spectrometry. The results show that 3.4% and 10.4% of polar brominated DBPs (in terms of total ion intensity) in the chlorinated Suwannee River fulvic acid and humic acid samples, respectively, penetrated through the AC, and 19.6% of polar brominated DBPs in the chlorinated secondary wastewater effluent sample penetrated through the AC. The ozone-modification of AC minimized the penetration of polar brominated DBPs during the TOBr analysis.
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Bone marrow transplantation combined with mesenchymal stem cells induces immune tolerance without cytotoxic conditioning.
J. Surg. Res.
PUBLISHED: 03-21-2011
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Transplantation of allogeneic donor bone marrow (BM) into sufficiently conditioned recipients is an effective approach to induce immune tolerance as assessed by mixed chimerism. However, this approach is hampered by the lack of feasible protocols devoid of cytoreductive conditioning. We investigated whether mixed chimerism could be established by intra-bone marrow-bone marrow transplantation (IBM-BMT) combined with bone marrow-derived mesenchymal stem cells (BMSCs) treatment without additional cytoreductive conditioning.
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Wnt/?-catenin pathway forms a negative feedback loop during TGF-?1 induced human normal skin fibroblast-to-myofibroblast transition.
J. Dermatol. Sci.
PUBLISHED: 01-07-2011
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Fibroblast-to-myofibroblast transition is a key event during wound healing and hypertrophic scar formation. Previous studies suggested Wnt/?-catenin signaling might be involved in the wound healing. However, its specific role in skin fibroblast-to-myofibroblast transition remains unclear.
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[Cytobiological effect of adipose-derived stem cells treated with insulin on HaCaT cells].
Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi
PUBLISHED: 07-15-2009
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To isolate and culture adipose-derived stem cells (ADSCs), and to study the effects of the conditioned medium of ADSCs (ADSC-CM) treated with insulin on HaCaT cells.
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In vivo evaluation of the anti-asthmatic, antitussive and expectorant activities of extract and fractions from Elaeagnus pungens leaf.
J Ethnopharmacol
PUBLISHED: 06-25-2009
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The leaf of Elaeagnus pungens thunb. (Family Elaeagnaceae) has been documented as an effective herb for the treatment of asthma and chronic bronchitis in traditional Chinese medicine. This study was aimed at evaluating the anti-asthmatic, antitussive and expectorant activities in vivo of the ethanolic extract and fractions from the leaf of Elaeagnus pungens. The results showed that the 70% ethanolic extract increased the preconvulsive time of asthma induced by the combination of histamine and acetylcholine chloride in guinea pigs at the medium dose of 1.379 g/kg. The water fraction significantly prolonged the preconvulsive time (P<0.05) in guinea pigs, lengthened the latent period of cough (P<0.05) as well as decreased the cough frequency caused by citric acid in guinea pigs (P<0.01) and enhanced tracheal phenol red output in mice (P<0.01). Moreover, the petroleum ether fraction significantly reduced the cough frequency induced in guinea pigs (P<0.01) and improved tracheal phenol red output in mice (P<0.01). It indicated that the petroleum ether fraction and the water fraction of Elaeagnus pungens leaf were effective on anti-asthmatic, antitussive and expectorant activities, which supplied for further research on chemical constituents and pharmacological mechanisms.
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Bone marrow mesenchymal stem cells upregulate transient outward potassium currents in postnatal rat ventricular myocytes.
J. Mol. Cell. Cardiol.
PUBLISHED: 02-15-2009
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Bone marrow mesenchymal stem cell (BMSC) transplantation has been shown to effectively improve cardiac function in experimental animals and patients with myocardial infarction and heart hypertrophy. BMSCs exert potent effects on cardiomyocytes through the inhibition of cardiac apoptosis, the attenuation of cardiac inflammation, etc. However, novel biological actions of BMSCs on cardiomyocytes remain to be explored. The present study was designed to investigate whether BMSCs affect electrophysiological features of neonatal rat ventricular myocytes (NRVMs). BMSCs and NRVMs were indirectly co-cultured at a ratio of 1:10 with a semi-permeable membrane. We found that compared with mono-cultured NRVMs, co-cultured NRVMs exhibited an obvious increase of transient outward potassium current (I(to)), accompanied by significant changes in activation, inactivation and recovery of I(to). Meanwhile, K(V)4.2 mRNA which encodes the channel carrying I(to) was more abundant in co-cultured NRVMs than mono-cultured NRVMs. The increases in basic fibroblast growth factor (bFGF) and insulin growth factor-1 (IGF-1) levels were observed in culture medium of BMSCs. bFGF but not IGF-1 upregulated the K(V)4.2 mRNA expression and enhanced I(to) currents. Taken together, we conclude that BMSCs upregulate I(to) of NRVMs, at least partially, by secreting bFGF that in turn upregulates K(V)4.2 expression and alters the kinetics of I(to).
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Role of donor-specific regulatory T cells in long-term acceptance of rat hind limb allograft.
PLoS ONE
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Vascularized bone marrow transplantation (VBMT) is widely accepted as an efficient means of establishing chimerism and inducing tolerance. However, the mechanism underlying is poorly understood. Recently, regulatory T cells (Tregs) have been shown to play an important role in regulating immune responses to allogeneic antigens. In this study, we explored the role of Tregs in the induction of tolerance in an allogeneic hind limb transplantation model.
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Synthesis of graphene peroxide and its application in fabricating super extensible and highly resilient nanocomposite hydrogels.
ACS Nano
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Functionalized graphene has been considered as one of the most important materials for preparing polymer nanocomposites due to its unique physical structure and properties. To increase the interfacial interaction between polymer component and graphene oxide (GO) sheets, in situ grafting polymerization initiated by a free radical initiator immobilized on GO sheets is a better choice. We report a facile and effective strategy for preparing graphene peroxide (GPO) via the radiation-induced peroxidation of GO. The formation of peroxides on GO is proven by iodometric measurement and other characterizations. Using GPO as a polyfunctional initiating and cross-linking center, we obtained GO composite hydrogels exhibiting excellent mechanical properties, namely, very high tensile strength (0.2-1.2 MPa), extremely high elongations (2000-5300%), and excellent resilience. This work provides new insight into the fabrication of GO/polymer nanocomposites to fulfill the excellent mechanical properties of graphene.
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[Functional divergence of betaine aldehyde dehydrogenase genes in Populus euphratica].
Sheng Wu Gong Cheng Xue Bao
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Plant betaine aldehyde dehydrogenase (BADH) is a physiologically important enzyme in response to salt or drought stress. In this study, two BADH genes (PeBADH1 and PeBADH2) were cloned from Populus euphratica. Both PeBADH1 and PeBADH2 genes encode the proteins of 503 amino acid residues, with a calculated molecular mass of 54.93 kDa and 54.90 kDa, respectively. Reverse transcription PCR showed the divergence of expression pattern between the PeBADH1 and PeBADH2 genes in P. euphratica. The recombinant PeBADH1 and PeBADH2 proteins were overexpressed in E. coli, and purified by Ni-affinity chromatography. The PeBADH2 protein had 1.5-fold higher enzymatic activity towards the substrate aldehyde than PeBADH1 protein. The PeBADH1 protein revealed higher thermal stability than PeBADH2 protein. These results indicated obvious functional divergence between the PeBADH1 and PeBADH2 genes.
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What is Visualize?

JoVE Visualize is a tool created to match the last 5 years of PubMed publications to methods in JoVE's video library.

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We use abstracts found on PubMed and match them to JoVE videos to create a list of 10 to 30 related methods videos.

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In developing our video relationships, we compare around 5 million PubMed articles to our library of over 4,500 methods videos. In some cases the language used in the PubMed abstracts makes matching that content to a JoVE video difficult. In other cases, there happens not to be any content in our video library that is relevant to the topic of a given abstract. In these cases, our algorithms are trying their best to display videos with relevant content, which can sometimes result in matched videos with only a slight relation.