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Find video protocols related to scientific articles indexed in Pubmed.
Expanding the crystal chemistry of uranyl peroxides: four hybrid uranyl-peroxide structures containing EDTA.
Inorg Chem
PUBLISHED: 10-28-2014
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The first four uranyl peroxide compounds containing ethylenediaminetetra-acetate (EDTA) were synthesized and characterized from aqueous uranyl peroxide nitrate solutions with a pH range of 5-7. Raman spectra demonstrated that reaction solutions that crystallized [NaK15[(UO2)8(O2)8(C10H12O10N2)2(C2O4)4]·(H2O)14] (1) and [Li4K6[(UO2)8(O2)6(C10H12O10N2)2(NO3)6]·(H2O)26] (2) contained excess peroxide, and their structures contained oxidized ethylenediaminetetraacetate, EDTAO2(4-). The solutions from which [K4[(UO2)4(O2)2(C10H13O8N2)2(IO3)2]·(H2O)16] (3) and LiK3[(UO2)4(O2)2(C10H12O8N2)2(H2O)2]·(H2O)18 (4) crystallized contained no free peroxide, and the structures incorporated intact EDTA(4-). In contrast to the large family of uranyl peroxide cage clusters, coordination of uranyl peroxide units in 1-4 by EDTA(4-) or EDTAO2(4-) results in isolated tetramers or dimers of uranyl ions that are bridged by bidentate peroxide groups. Two tetramers are bridged by EDTAO2(4-) to form octamers in 1 and 2, and dimers of uranyl polyhedra are linked through iodate groups in 3 and EDTA(4-) in 4, forming chains in both cases. In each structure the U-O2-U dihedral angle is strongly bent, at ?140°, consistent with the configuration of this linkage in cage clusters and other recently reported uranyl peroxides.
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[Peripheral arterial thromboembolism in patients with non valvular atrial fibrillation: a single center case-control study].
Zhonghua Xin Xue Guan Bing Za Zhi
PUBLISHED: 10-21-2014
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To explore the clinical characteristics and risk factors of peripheral arterial thromboembolism (PAT) in patients with non valvular atrial fibrillation (NVAF).
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[Function of CD4(+) CD25(+) regulatory T cells in Henoch-Schonlein purpura nephritis in children].
Zhonghua Er Ke Za Zhi
PUBLISHED: 09-17-2014
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To investigate the levels and functions of CD4(+)CD25(+) regulatory T cells and specific transcription factor Foxp3 and Th17 cells related cytokine in peripheral blood mononuclear cells (PBMC) and renal tissues, and explore their roles in pathogenesis of Henoch-Schonlein purpura nephropathy (HSPN) in children.
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Genome re-sequencing suggested a weedy rice origin from domesticated indica-japonica hybridization: a case study from southern China.
Planta
PUBLISHED: 09-04-2014
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Whole-genome re-sequencing of weedy rice from southern China reveals that weedy rice can originate from hybridization of domesticated indica and japonica rice. Weedy rice (Oryza sativa f. spontanea Rosh.), which harbors phenotypes of both wild and domesticated rice, has become one of the most notorious weeds in rice fields worldwide. While its formation is poorly understood, massive amounts of rice genomic data may provide new insights into this issue. In this study, we determined genomes of three weedy rice samples from the lower Yangtze region, China, and investigated their phylogenetics, population structure and chromosomal admixture patterns. The phylogenetic tree and principle component analysis based on 46,005 SNPs with 126 other Oryza accessions suggested that the three weedy rice accessions were intermediate between japonica and indica rice. An ancestry inference study further demonstrated that weedy rice had two dominant genomic components (temperate japonica and indica). This strongly suggests that weedy rice originated from indica-japonica hybridization. Furthermore, 22,443 novel fixed single nucleotide polymorphisms were detected in the weedy genomes and could have been generated after indica-japonica hybridization for environmental adaptation.
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[Karyotype analysis of three Lonicera species growth in Sichuan].
Zhong Yao Cai
PUBLISHED: 09-02-2014
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To provide the genetic reference for development and application of Lonicera species growth in Sichuan.
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[Responses of ecosystem services value to land use change in national nature reserves in Xinjiang, China].
Ying Yong Sheng Tai Xue Bao
PUBLISHED: 08-19-2014
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With GIS and RS technology, characteristics of land use change and ecosystem services value of different years in six national nature reserves in Xinjiang, China were analyzed with remote sensing image of the year 2000-2010. Results showed that the area of water body and grassland decreased while the area of forest, farmland, wetland, unused land and construction land increased in 2000-2010. Variation rate of land use change in 2000-2005 was faster, as 2.4-6.3 times as that in 2005-2010. The total ecosystem services value mostly consisted of that of water body, grassland and forest, accounting for 93% approximately. During the study, the values of all kinds of the ecosystem services increased except for grassland and water body, the total ecosystem services value increased firstly and then decreased, and overall emerged as a reducing trend with the rate of 1.2%. In the process of the development of the west regions, it is necessary to pay more attention to the protection of natural resources and ecosystem restoration, so as to achieve sustainable development of resources, environment and social economy in the western regions.
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Downregulation of Notch1 inhibits the invasion of human hepatocellular carcinoma HepG2 and MHCC97H cells through the regulation of PTEN and FAK.
Int. J. Mol. Med.
PUBLISHED: 08-08-2014
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Tumor invasion and metastasis are the main causes of mortality in patients with hepatocellular carcinoma (HCC). Thus, the effective inhibition of these tumorigenic processes is critical in order for HCC therapy to be effective. Previous studies have demonstrated that Notch1 is associated with metastasis in several human malignancies. However, the exact molecular mechanisms underlying the Notch1-mediated induction of the invasion of HCC cells remain poorly understood. In the present study, we demonstrate that, compared to the normal liver cell line, L02, Notch1 is highly expressed in the human HCC cell lines, HepG2 and MHCC97H. Using small interfering RNA (siRNA), we knocked down the expression of Notch1 in the cell lines. Notch1 expression in the HCC cell lines was also measured following transfection with siRNA using RT-PCR and western blot analysis. In addition, a migration and invasion assay was performed to determine the effects of Notch1 knockdown on cell migration and invasion. Our results demonstrated that the downregulation of Notch1 by small interfering RNA (siRNA) significantly inhibited the migration and invasion of both HCC cell lines. Additionally, we demonstrated that the knockdown of Notch1 in both HCC cell lines increased both the total expression of phosphatase and tensin homolog (PTEN) and its phosphorylated form. By contrast, focal adhesion kinase (FAK) and phospho-FAK expression was decreased following Notch1 depletion. Taken together, our data suggest that targeting Notch1 may be a useful therapeutic approach to inhibiting the metastasis of HCC cells.
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Perinatal mortality in pregnancies with omphalocele: data from the Chinese national birth defects monitoring network, 1996-2006.
BMC Pediatr
PUBLISHED: 06-12-2014
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Previous studies on the mortality rate of omphalocele are limited. The risk of death of non-isolated omphalocele and that of cases of omphalocele that are diagnosed prenatally by ultrasound are unclear. This study aimed to estimate the perinatal mortality of pregnancies with omphalocele. This study also examined the potential risk of death of non-isolated omphalocele and that of cases that are prenatally diagnosed by ultrasound.
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Increasing maternal percentage body fat in early second trimester: a risk factor for preeclampsia.
J. Matern. Fetal. Neonatal. Med.
PUBLISHED: 06-12-2014
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Abstract Objective: To determine if maternal percentage body fat (PBF) or fat free mass (FFM) in the early second trimester of pregnancy influenced the development of preeclampsia. Methods: A matched nested case-control study was conducted from a cohort study of 1668 women at Gansu provincial maternal and child care hospital from July 2007 to August 2011 in China. Maternal PBF and FFM were assessed by bioelectrical impedance analysis during 12th-16th gestational week. The demographic characteristics were all chart abstracted. After childbirth, 70 cases of preeclampsia were matched by race/age with 140 uncomplicated pregnancies women. Multivariate logistic regression analysis was performed to determine the associated risk factors. Results: Pre-pregnancy body mass index were higher in women who subsequently developed preeclampsia compared with controls (p?
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Metformin prevents LYRM1-induced insulin resistance in 3T3-L1 adipocytes via a mitochondrial-dependent mechanism.
Exp. Biol. Med. (Maywood)
PUBLISHED: 06-07-2014
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We previously proposed that LYR motif containing 1 (LYRM1)-induced mitochondrial reactive oxygen species (ROS) production contributes to obesity-related insulin resistance. Metformin inhibits ROS production and promotes mitochondrial biogenesis in specific tissues. We assessed the effects of metformin on insulin resistance in LYRM1-over-expressing 3T3-L1 adipocytes. Metformin enhanced basal and insulin-stimulated glucose uptake and GLUT4 translocation, reduced IRS-1 and Akt phosphorylation and ROS levels, and affected the expression of regulators of mitochondrial biogenesis in LYRM1-over-expressing adipocytes. Metformin may ameliorate LYRM1-induced insulin resistance and mitochondrial dysfunction in part via a direct antioxidant effect and in part by activating the adenosine monophosphate-activated protein kinase (AMPK)-PGC1/NRFs pathway.
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Proliferating cell nuclear antigen involved in the repair process of ouabain-induced brain damage independent of hypertension in rats.
Chin. Med. J.
PUBLISHED: 06-04-2014
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Ouabain is a mammalian adrenocortical hormone that is involved in the pathogenesis of hypertension by inhibiting Na-K ATPase activity. It also participates in a variety of kinase-mediated signaling pathways associated with Na-K ATPase. Previous studies have shown that ouabain can cause cardiac remodeling independent of elevated blood pressure and that proliferating cell nuclear antigen (PCNA) plays a coordinating role for numerous proteins involved in multiple processes associated with DNA synthesis. Therefore, we hypothesized that ouabain might play a role in the cerebral cortex through signaling pathways independent of hypertension. And PCNA might be involved in this process.
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[Changes and influencing factors of the soil organic carbon in farmland in the last 30 years on Hilly Loess Plateau: a case study in Zhuanglang County, Gansu Province].
Huan Jing Ke Xue
PUBLISHED: 06-03-2014
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By analysing the sampled data and the results of the second national soil survey by the mid 80 s in Zhuanglang County, the article studied on the changes and influencing factors of the soil organic carbon in farmland of this area in the last 30 years. Farmland samples of top soil (0-20 cm) were collected and analyzed in July 2011. The results showed that (1) The average contents of the soil organic carbon in the county's farmlands were 6.80 g x kg(-1) in 1985 and 8.90 g x kg(-1) in 2011. It increased by 30.9% in the past 30 years, which appeared as a carbon sink effect. The area of increasing contents of soil organic carbon accounted for about 90% of the county's farmland area. (2) Under the available management measures and farmland input, the loessal soil organic carbon stability level was 11.0 g x kg(-1), The SOC accumulation rate showed that the farther the SOC was from the stability level the more quickly it changed, and the closer the SOC was from the stability level the slower it changed. (3) The SOC changes was affected by the altitude, the primary content of organic carbon, and the soil types and so on, in which the greatest contribution factor was the altitude, the influence of the primary content of organic carbon, soil type, production and organic fertilizer on soil organic carbon change was smaller, and the slope aspect had the smallest effect on soil organic carbon change.
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Passive smoking and preterm birth in urban China.
Am. J. Epidemiol.
PUBLISHED: 05-17-2014
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Studies investigating the relationship between maternal passive smoking and the risk of preterm birth have reached inconsistent conclusions. A birth cohort study that included 10,095 nonsmoking women who delivered a singleton live birth was carried out in Lanzhou, China, between 2010 and 2012. Exposure to passive smoking during pregnancy was associated with an increased risk of very preterm birth (<32 completed weeks of gestation; odds ratio = 1.98, 95% confidence interval: 1.41, 2.76) but not moderate preterm birth (32-36 completed weeks of gestation; odds ratio = 0.98, 95% confidence interval: 0.81, 1.19). Risk of very preterm birth increased with the duration of exposure (P for trend = 0.0014). There was no variability in exposures by trimester. The associations were consistent for both medically indicated and spontaneous preterm births. Overall, our findings support a positive association between passive smoking and the risk of very preterm birth.
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Knockdown of LYRM1 rescues insulin resistance and mitochondrial dysfunction induced by FCCP in 3T3-L1 adipocytes.
Cell Biochem. Biophys.
PUBLISHED: 04-29-2014
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LYR motif-containing 1 (LYRM1) was recently discovered to be involved in adipose tissue homeostasis and obesity-associated insulin resistance. We previously demonstrated that LYRM1 overexpression might contribute to insulin resistance and mitochondrial dysfunction. Additionally, knockdown of LYRM1 enhanced insulin sensitivity and mitochondrial function in 3T3-L1 adipocytes. We investigated whether knockdown of LYRM1 in 3T3-L1 adipocytes could rescue insulin resistance and mitochondrial dysfunction induced by the cyanide p-trifluoromethoxyphenyl-hydrazone (FCCP), a mitochondrion uncoupler, to further ascertain the mechanism by which LYRM1 is involved in obesity-associated insulin resistance. Incubation of 3T3-L1 adipocytes with 1 µM FCCP for 12 h decreased insulin-stimulated glucose uptake, reduced intracellular ATP synthesis, increased intracellular reactive oxygen species (ROS) production, impaired insulin-stimulated Glucose transporter type 4 (GLUT4) translocation, and diminished insulin-stimulated tyrosine phosphorylation of Insulin receptor substrate-1 (IRS-1) and serine phosphorylation of Protein Kinase B (Akt). Knockdown of LYRM1 restored insulin-stimulated glucose uptake, rescued intracellular ATP synthesis, reduced intracellular ROS production, restored insulin-stimulated GLUT4 translocation, and rescued insulin-stimulated tyrosine phosphorylation of IRS-1 and serine phosphorylation of Akt in FCCP-treated 3T3-L1 adipocytes. This study indicates that FCCP-induced mitochondrial dysfunction and insulin resistance are ameliorated by knockdown of LYRM1.
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In situ quantification and visualization of lithium transport with neutrons.
Angew. Chem. Int. Ed. Engl.
PUBLISHED: 04-10-2014
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A real-time quantification of Li transport using a nondestructive neutron method to measure the Li distribution upon charge and discharge in a Li-ion cell is reported. By using in?situ neutron depth profiling (NDP), we probed the onset of lithiation in a high-capacity Sn anode and visualized the enrichment of Li atoms on the surface followed by their propagation into the bulk. The delithiation process shows the removal of Li near the surface, which leads to a decreased coulombic efficiency, likely because of trapped Li within the intermetallic material. The developed in?situ NDP provides exceptional sensitivity in the temporal and spatial measurement of Li transport within the battery material. This diagnostic tool opens up possibilities to understand rates of Li transport and their distribution to guide materials development for efficient storage mechanisms. Our observations provide important mechanistic insights for the design of advanced battery materials.
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Prediction of antiviral efficacy in patients with chronic hepatitis C by changes in forkhead box protein 3 levels.
Exp Ther Med
PUBLISHED: 01-31-2014
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The aim of the present study was to investigate the distribution of CD4(+)CD25(+) regulatory T cells (Tregs) in the peripheral blood of patients with chronic hepatitis C; in addition to identifying whether the distribution of CD4(+)CD25(+) Tregs predicts the efficacy of antiviral therapy for HCV. The expression of CD4(+)CD25(+) forkhead box protein (FOXP) 3(+) Tregs within a CD4(+) T cell population was detected in the peripheral blood obtained from patients with chronic hepatitis C and from healthy control subjects using flow cytometry. The hepatitis C virus (HCV)-RNA load was measured using quantitative-fluorescence polymerase chain reaction. CD4(+)CD25(+)FOXP3(+) Tregs accounted for 14.24±1.33% of the CD4(+) T cells in the peripheral blood of patients with chronic hepatitis C, which was higher than that of the healthy control subjects (5.62±1.21%; P<0.001). Furthermore, the frequency of CD4(+)CD25(+)FOXP3(+) Tregs in CD4(+) T cells of the peripheral blood positively correlated with the HCV-RNA load (r=0.73; P=0.032). Therefore, the results of the present study indicated that the expression of CD4(+)CD25(+)FOXP3(+) Tregs increased in patients that were chronically infected with HCV and positively correlated with the HCV-RNA load.
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Prenatal exposure to organochlorine pesticides and infant birth weight in China.
Chemosphere
PUBLISHED: 01-25-2014
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In utero exposure to organochlorine pesticides (OCPs) is thought to be potentially harmful to fetal development. We aimed to investigate the associations of maternal and cord serum OCPs levels with infant birth weight in China. In this study, we measured serum levels of 18 OCPs in 81 mother-infant pairs, including DDT, hexachlorocyclohexanes (BHC), hexachlorobenzene (HCB), heptachlors, chlordanes, endosulfan-I, and mirex using a high-resolution-gas-chromatography with high-resolution-mass-spectrometry method. We found that p,p'-DDE and ?-BHC had the highest detection rate in both maternal and cord blood serum (97.2% and 96.7%, respectively), followed by HCB (93.0%, 51.7%), p,p'-DDT (88.7%, 36.7%), and p,p'-DDD (83.1%, 60.0%). Among all OCPs, the concentration of p,p'-DDE was the highest (mothers geometric mean (GM): 203.54ngg(-1), newborns GM: 116.14ngg(-1)), followed by HCB (70.62ngg(-1), 65.16ngg(-1)), and ?-BHC (67.67ngg(-1), 33.39ngg(-1)). Multiple linear regression analyses showed that each 1ngg(-1) increment of cord serum p,p'-DDE, total DDT, and ?-BHC was associated with a 0.10g, 0.10g, and 0.92g decrease in infant birth weight, respectively, and as the cord serum concentrations of p,p'-DDT, p,p'-DDD, HCB and mirex increased, the infant birth weight was also decreased, although the associations were not statistically significant due to the relatively small sample size. These results suggest that p,p'-DDE, ?-BHC, and HCB were the predominant OCPs in the serum of Chinese pregnant women and cord blood of their newborns. Prenatal exposure to DDT, ?-BHC, HCB, and mirex were associated with a decrease in birth weight, but these results need validation in larger sample-sized studies.
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Fatty acid synthase (FASN) levels in serum of colorectal cancer patients: correlation with clinical outcomes.
Tumour Biol.
PUBLISHED: 01-17-2014
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Fatty acid synthase (FASN) is a common phenotype to many kinds of human cancers, such as those of the breast, ovary, pancreas, prostate, colon, and so on. Increased FASN levels have been detected in the serum of the patients with breast and pancreatic cancers. The relationship between the FASN level in serum and the clinicopathological characteristics of colorectal cancer is investigated in this study. FASN levels in serum were examined with enzyme-linked immunosorbent assay (ELISA) in 74 patients with colorectal cancer and 40 healthy persons. Pathological and clinical factors associated with FASN concentrations in serum were investigated and analyzed by statistical analysis. The FASN level in colorectal cancer patients' serum is significantly higher than that in healthy persons' serum. FASN levels in the serum of colorectal cancer patients are associated with tumor extent, lymph node metabasis status, distant metastasis, and tumor clinical stage. The 5-year overall survival rate and 5-year disease-free survival rate among patients with low FASN levels in serum are significantly higher than those among patients with high FASN levels in serum (log-rank P = 0.003). The high FASN level in serum is a promising independent predictor of colorectal cancers with advanced phases, late clinical stages, and shorter survival. These results suggest that FASN concentration in serum may be a potential and useful tumor marker.
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MicroRNA?210 knockdown contributes to apoptosis caused by oxygen glucose deprivation in PC12 cells.
Mol Med Rep
PUBLISHED: 01-09-2014
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It was previously demonstrated that microRNA-210 (miR-210) exhibited neuroprotective effects in a murine model of hypoxic?ischemic encephalopathy via inhibition of apoptosis. The aim of the present study was to further elucidate the effect of miR-210 on apoptosis in PC12 cells following transfection with miR?210 inhibitors and exposure to oxygen glucose depri-vation (OGD). The expression levels of miR?210 were identified using reverse transcription?quantitative polymerase chain reaction analysis. Apoptosis was investigated using Annexin V?fluorescein isothiocyanate assays. Apoptosis?related protein expression levels were studied with western blot analysis. The results showed that the expression levels of miR?210 were upregulated in PC12 cells following a 4?h exposure to OGD, relative to those in normoxic control cells. miR?210 knockdown increased cell apoptosis by inducing caspase activity and regulating the balance between Bcl?2 and Bax levels. The present study demonstrated that miR?210 knockdown induced cell apoptosis using an ex vivo model of ischemic hypoxia (IH). Knockdown of miR?210 represents a potential novel therapeutic approach to combat neonatal IH.
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Profilin-1 promotes the development of hypertension-induced artery remodeling.
J. Histochem. Cytochem.
PUBLISHED: 01-07-2014
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Hypertension is associated with the structural remodeling and stiffening of arteries and is known to increase cardiovascular risk. In the present study, we investigated the effects of overexpression and knock down of profilin-1 on the vascular structural remodeling in spontaneous hypertensive rats (SHRs) using an adenovirus injection to knock down or overexpress profilin-1 mRNA. As a control, blank adenovirus was injected into age-matched SHRs and Wistar-Kyoto rats (WKYs). We quantified arterial structural remodeling through morphological methods, with thoracic aortas stained with hematoxylin-eosin and picosirius red. Western blotting was performed to measure the protein expression of inducible nitric oxide synthase (iNOS) and p38 mitogen-activated protein kinase (p38), and peroxynitrite was quantified by immunohistochemical staining. Overexpression of profilin-1 significantly promoted aortic remodeling, including an increase in vessel size, wall thickness, and collagen content, whereas the knockdown of profilin-1 could reverse these effects. In addition, the expression of phosphorylated p38, iNOS and peroxynitrite was significantly upregulated in SHRs with profilin-1 overexpression along with an increased level of interleukin- 6 (IL-6). These changes could be reversed by knockdown of profilin-1. Our results demonstrate a crucial role for profilin-1 in hypertension-induced arterial structural remodeling at least in part through the p38-iNOS-peroxynitrite pathway.
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Regulation of pulmonary surfactant synthesis in fetal rat type II alveolar epithelial cells by microRNA-26a.
Pediatr. Pulmonol.
PUBLISHED: 01-03-2014
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Pulmonary surfactant, a unique developmentally regulated, phospholipid-rich lipoprotein, is synthesized by the type II epithelial cells (AECII) of the pulmonary alveolus, where it is stored in organelles termed lamellar bodies. The synthesis of pulmonary surfactant is under multifactorial control and is regulated by a number of hormones and factors, including glucocorticoids, prolactin, insulin, growth factors, estrogens, androgens, thyroid hormones, and catecholamines acting through beta-adrenergic receptors, and cAMP. While there is increasing evidence that microRNAs (miRNAs) are involved in the regulation of almost every cellular and physiological process, the potential role of miRNAs in the regulation of pulmonary surfactant synthesis remains unknown. miRNA-26a (miR-26a) has been predicted to target SMAD1, one of the bone morphogenetic protein (BMP) receptor downstream signaling proteins that plays a key role in differentiation of lung epithelial cells during lung development. In this study, we explored the regulation role of miR-26a in the synthesis of pulmonary surfactant. An adenoviral miR-26a overexpression vector was constructed and introduced into primary cultured fetal AECII. GFP fluorescence was observed to determinate the transfection efficiency and miR-26a levels were measured by RT-PCR. MTT was performed to analyze AECII viability. qRT-PCR and Western blotting were used to determine the mRNA and protein level of SMAD1 and surfactant-associated proteins. The results showed that miR-26a in fetal AECII was overexpressed after the transfection, and that the overexpression of miR-26a inhibited pulmonary surfactant synthesis in AECII. There was no significant change in cell proliferation. Our results further showed that overexpression of miR-26a reduced the SMAD1 expression both in mRNA and protein level in fetal AECII. These findings indicate that miR-26a regulates surfactant synthesis in fetal AECII through SMAD1.
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Genome re-sequencing of semi-wild soybean reveals a complex Soja population structure and deep introgression.
PLoS ONE
PUBLISHED: 01-01-2014
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Semi-wild soybean is a unique type of soybean that retains both wild and domesticated characteristics, which provides an important intermediate type for understanding the evolution of the subgenus Soja population in the Glycine genus. In this study, a semi-wild soybean line (Maliaodou) and a wild line (Lanxi 1) collected from the lower Yangtze regions were deeply sequenced while nine other semi-wild lines were sequenced to a 3-fold genome coverage. Sequence analysis revealed that (1) no independent phylogenetic branch covering all 10 semi-wild lines was observed in the Soja phylogenetic tree; (2) besides two distinct subpopulations of wild and cultivated soybean in the Soja population structure, all semi-wild lines were mixed with some wild lines into a subpopulation rather than an independent one or an intermediate transition type of soybean domestication; (3) high heterozygous rates (0.19-0.49) were observed in several semi-wild lines; and (4) over 100 putative selective regions were identified by selective sweep analysis, including those related to the development of seed size. Our results suggested a hybridization origin for the semi-wild soybean, which makes a complex Soja population structure.
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Value assessment of ecosystem services in nature reserves in Ningxia, China: a response to ecological restoration.
PLoS ONE
PUBLISHED: 01-01-2014
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Changes in land use can cause significant changes in the ecosystem structure and process variation of ecosystem services. This study presents a detailed spatial, quantitative assessment of the variation in the value of ecosystem services based on land use change in national nature reserves of the Ningxia autonomous region in China. We used areas of land use types calculated from the remote sensing data and the adjusted value coefficients to assess the value of ecosystem services for the years 2000, 2005, and 2010, analyzing the fluctuations in the valuation of ecosystem services in response to land use change. With increases in the areas of forest land and water bodies, the value of ecosystem services increased from 182.3×10(7) to 223.8×10(7) US$ during 2000-2010. Grassland and forest land accounted for 90% of this increase. The values of all ecosystem services increased during this period, especially the value of ecosystem services for biodiversity protection and soil formation and protection. Ecological restoration in the reserves had a positive effect on the value of ecosystem services during 2000-2010.
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[Prevalence of chronic obstructive pulmonary disease in Ningxia Hui Autonomous Region of China].
Zhonghua Jie He He Hu Xi Za Zhi
PUBLISHED: 08-16-2013
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To investigate the prevalence of chronic obstructive pulmonary disease (COPD) and its current status of diagnosis and management in Ningxia Hui Autonomous Region of China.
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Combination of human leukocyte antigen and killer cell immunoglobulin-like receptor genetic background influences the onset age of hepatocellular carcinoma in male patients with hepatitis B virus infection.
Clin. Dev. Immunol.
PUBLISHED: 07-15-2013
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To investigate whether killer cell immunoglobulin-like receptor (KIR) and human leukocyte antigen (HLA) genetic background could influence the onset age of hepatocellular carcinoma (HCC) in patients with hepatitis B virus (HBV) infection, one hundred and seventy-one males with HBV-related HCC were enrolled. The presence of 12 loci of KIR was detected individually. HLA-A, -B, and -C loci were genotyped with high resolution by a routine sequence-based typing method. The effect of each KIR locus, HLA ligand, and HLA-KIR combination was examined individually by Kaplan-Meier (KM) analysis. Multivariate Cox hazard regression model was also applied. We identified C1C1-KIR2DS2/2DL2 as an independent risk factor for earlier onset age of HCC (median onset age was 44 for C1C1-KIR2DS2/2DL2 positive patients compared to 50 for negative patients, P = 0.04 for KM analysis; HR = 1.70, P = 0.004 for multivariate Cox model). We conclude that KIR and HLA genetic background can influence the onset age of HCC in male patients with HBV infection. This study may be useful to improve the current HCC surveillance program in HBV-infected patients. Our findings also suggest an important role of natural killer cells (or other KIR-expressing cells) in the progress of HBV-related HCC development.
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The psychometric properties of the Chinese version of the Conditions of Work Effectiveness Questionnaire-II.
J Nurs Manag
PUBLISHED: 07-02-2013
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The present study was conducted to evaluate the psychometric properties of a newly adapted Chinese version of an instrument designed to measure structural empowerment among staff nurses.
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Low-dose-area-constrained helical TomoTherapy-based whole breast radiotherapy and dosimetric comparison with tangential field-in-field IMRT.
Biomed Res Int
PUBLISHED: 06-15-2013
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To present a novel helical TomoTherapy-based method for whole breast radiotherapy that has better dosimetry and also has acceptable low-dose regions for lungs, heart, and contralateral breast compared with tangential field-in-field IMRT (FIF-IMRT).
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Hybrid uranyl-carboxyphosphonate cage clusters.
Inorg Chem
PUBLISHED: 06-13-2013
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Two new hybrid uranyl-carboxyphosphonate cage clusters built from uranyl peroxide units were crystallized from aqueous solution under ambient conditions in approximately two months. The clusters are built from uranyl hexagonal bipyramids and are connected by employing a secondary metal linker, the 2-carboxyphenylphosphonate ligand. The structure of cluster A is composed of a ten-membered uranyl polyhedral belt that is capped on either end of an elongated cage by five-membered rings of uranyl polyhedra. The structure of cluster B consists of 24 uranyl cations that are arranged into 6 four-membered rings of uranyl polyhedra. Four of the corresponding topological squares are fused together to form a sixteen-membered double uranyl pseudobelt that is capped on either end by 2 topological squares. Cluster A crystallizes over a wide pH range of 4.6-6.8, while cluster B was isolated under narrower pH range of 6.9-7.8. Studies of their fate in aqueous solution upon dissolution of crystals by electrospray ionization mass spectrometry (ESI-MS) and small-angle X-ray scattering (SAXS) provide evidence for their persistence in solution. The well-established characteristic fingerprint from the absorption spectra of the uranium(VI) cations disappears and becomes a nearly featureless peak; nonetheless, the two compounds fluoresce at room temperature.
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Association of human leukocyte antigen class I polymorphism with spontaneous clearance of hepatitis B surface antigen in qidong han population.
Clin. Dev. Immunol.
PUBLISHED: 05-29-2013
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Aim. To investigate whether HLA class I polymorphisms could influence the clearance of hepatitis B surface antigen (HBsAg) in Qidong Han population. Methods. We genotyped HLA-A, -B, and -C loci of 448 individuals with HBV persistent infection and 140 persons with spontaneous clearance of HBsAg by polymerase chain reaction with sequencing based typing (PCR/SBT). All the individuals were unrelated males enrolled from Qidong Han population and were followed up for 10 years. Results. The frequency of HLA-A?33:03:01G was increased in persistent HBV infection group (P value is 0.028), while frequency of HLA-B?13:01:01G was increased in HBsAg clearance group (P value is 0.0004). Conclusion. These findings suggested that the host HLA class I polymorphism is an important factor in determining the outcomes of HBV infection.
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Tunable microwave frequency performance of nanocomposite Co2MnSi/PZN-PT magnetoelectric coupling structure.
J Nanosci Nanotechnol
PUBLISHED: 05-08-2013
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Nanocrystalline Co2MnSi Heusler alloy films were deposited on the PZN-PT substrates by a composition gradient sputtering method. It is revealed that this multiferroic heterostructure shows very strong magnetoelectric coupling, leading to continuously tunable microwave frequency characteristics by electric field. With the increase of electric field intensity from 0 to 6 kV/cm, the magnetic anisotropy field H(K) increases from 90 Oe to 182 Oe with an increment of 102%, corresponding to a ME coefficient of 15.3 Oe cm/kV; the ferromagnetic resonance frequency f(FMR) shifts from 3.38 to 4.82 GHz with an increment of deltaf(FMR) = 1440 MHz or deltaf(FMR)/f(FMR) = 43%; moreover, the damping constant alpha dramatically decreases from 0.035 to 0.018. These merits demonstrate that this nanocomposite multiferroic structure is promising in fabrication of tunable microwave components.
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Profilin-1 promotes the development of hypertension-induced cardiac hypertrophy.
J. Hypertens.
PUBLISHED: 04-26-2013
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Cardiac hypertrophy is a major cause of heart failure and sudden cardiac death among hypertensive individuals. The present study examined the effects of profilin-1 on hypertension-induced cardiac hypertrophy.
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Neuroprotective effects of microRNA-210 on hypoxic-ischemic encephalopathy.
Biomed Res Int
PUBLISHED: 04-22-2013
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To reveal the effect of microRNA-210 on cell apoptosis caused by HIE.
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Neuroprotective effects of microRNA-210 against oxygen-glucose deprivation through inhibition of apoptosis in PC12 cells.
Mol Med Rep
PUBLISHED: 04-10-2013
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Although several reports have demonstrated the specific roles of microRNAs (miRs) in neuronal differentiation, neurogenesis, neural cell specification and neurodevelopmental function, there have been no studies with regard to the importance of miRs in hypoxic-ischemic encephalopathy (HIE). In the present study, we aimed to investigate the effect of miR-210 on neuronal cell apoptosis caused by HI injury. We established an ex vivo model of HIE using oxygen-glucose deprivation (OGD) and demonstrated that miR-210 expression was upregulated in pheochromocytoma (PC12) cells after 4 h of OGD compared with normoxic controls. Furthermore, miR-210 suppressed cell apoptosis by inhibiting caspase activity and by regulating the balance between Bcl-2 and Bax levels. In conclusion, the present study revealed that miR-210 exerts neuroprotective effects by inhibiting cell apoptosis. This work represents a potential novel therapeutic approach to combat neonatal HI injury.
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Overexpression of C10orf116 promotes proliferation, inhibits apoptosis and enhances glucose transport in 3T3-L1 adipocytes.
Mol Med Rep
PUBLISHED: 02-27-2013
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Data from our previous study demonstrated that C10orf116 is an adipocyte lineage-specific nuclear factor, which regulates master adipogenesis transcription factors during early differentiation. However, the precise functional properties of this gene have yet to be identified and further investigation is required. In the present study, we report the effects of C10orf116 expression on cell proliferation and apoptosis in vitro and observed that the overexpression of C10orf116 stimulates proliferation and inhibits apoptosis in preadipocytes. Furthermore, we investigated the effects of C10orf116 on glucose uptake and demonstrated that the ectopic expression of C10orf116 significantly increases insulin-stimulated glucose uptake in adipocytes by increasing glucose transporter type 4 (GLUT4) expression levels. Collectively, these data further support the hypothesis that C10orf116 is important in regulating glucose transport in adipocytes as well as the number of preadipocytes. The results of the present study may also provide insights into the complex mechanisms involved in the development of obesity.
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Polymorphism of angiotensinogen gene M235T in myocardial infarction and brain infarction: a meta-analysis.
Gene
PUBLISHED: 02-06-2013
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The angiotensinogen (AGT) gene M235T polymorphism has been reported to be associated with myocardial infarction (MI) and brain infarction (BI), but the results remain inconclusive. This meta-analysis was designed to clarify these controversies. Electronic databases were systematically searched before February 2013. A total of 38 studies with 17304 subjects met our inclusion criteria. In East Asian group, significant association was found between AGT M235T polymorphism and risk of MI (for dominant model: OR=1.79; 95% CI=1.04-3.06; for recessive model OR=2.01; 95% CI=1.21-3.36; for additive model OR=1.79; 95% CI=1.14-2.86) as well as BI (for dominant model: OR=1.66; 95% CI=1.22-2.27; for recessive model OR=1.78, 95% CI=1.29-2.46; for additive model: OR=1.64, 95% CI=1.34-2.00), while the M235T polymorphism did not impact the risk of MI in total population and other ethnicity. In the subgroup analyses by gender and age, there was lack of evidence for the association. This meta-analysis suggested an association between the M235T polymorphism and MI as well as BI in East Asian population. Further studies with larger numbers of worldwide participants are needed to understand the genetic basis of MI and BI.
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Human leukocyte antigen class I alleles and haplotypes associated with primary hepatocellular carcinoma in persistent HBV-infected patients.
Hum. Immunol.
PUBLISHED: 01-18-2013
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Many studies have shown that Human leukocyte antigen (HLA) class I alleles are associated with the development of various cancers. However, its role in hepatocellular carcinoma (HCC) is still unknown. To investigate whether HLA class I allelic polymorphism is related to the development of hepatitis B virus(HBV)-associated HCC, a total of 326 HBV-infected patients (138 individuals with HCC and 188 well-matched controls without HCC) were enrolled in this study. HLA-A, -B, and -C were genotyped by polymerase chain reaction-sequencing based typing (PCR-SBT) method. We identified HLA-B(?)35:01:01G as a risk factor for HBV-related HCC development independent of our previous findings in HLA region (OR, 12.04; p, 0.0028; pc, 0.04). HLA-A(?)11:01:01G, B(?)58:01:01G, C(?)03:02:01G and some of their extended haplotypes were found as potential susceptible factors for HCC development. HLA-B(?)46:01:01G and some of its extended haplotypes were found as potential protective factors for HCC development. Our results support that specific HLA class I alleles and haplotypes may affect the risk of HBV-related HCC development. The findings may help to determine better approaches for prevention and treatment of HCC in these patients.
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The expression profile of microRNAs in wistar rats with lipopolysaccharide-induced periventricular leukomalacia.
J. Mol. Neurosci.
PUBLISHED: 01-09-2013
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Over the recent decades, with numbers of premature infants being cured, clinical diseases on brain damage like periventricular leukomalacia (PVL) have become much more common. Meanwhile, since the discovery of first miRNA lin-4, an increasing number of important studies about this small RNA have been performed not only in the normal organ development but also in the pathogenic mechanism of diseases. However, throughout the past several years, there have been rare miRNA researches discussing the connection between the PVL and miRNA. In view of this situation, we constructed an animal model of PVL induced by lipopolysaccharide (LPS) and performed a miRNA microarray which was repeated three times to profile the expression of microRNAs (miRNAs) between two groups (PVL group versus control group). Then, miRNAs with notable fold changes (fold change >1.5) were found; some of them were further validated by real-time PCR. As a result, 104 differentially expressed miRNAs were identified using the microarray, including 64 upregulated and 40 downregulated miRNAs. Then, five miRNAs of them were selected, characterized by consistent trend in expression in all three microarrays. Among these five miRNAs (miRNA-451, miRNA-200b, miRNA-29a, miRNA-21, and miRNA-138), we subsequently selected miRNA-451 and miRNA-200b for real-time PCR because they possess the highest fold changes. Finally, the results of PCR are basically in accord with the microarray. We guess these new identified miRNAs may play an important role in the pathogenesis of PVL and may provide certain pathophysiological basis for the future research of related diseases in preterm infants.
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Characterization of microRNA expression profiles in 3T3-L1 adipocytes overexpressing C10orf116.
Mol. Biol. Rep.
PUBLISHED: 01-08-2013
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Our data in the previous report demonstrated that C10orf116 (AFRO) is an adipocyte lineage-specific nuclear factor that can modulate the master adipogenesis transcription factors early during differentiation. However, more precise functional properties of this gene need to be clarified and await further investigation. Therefore, in this study, we performed an expression profile of cellular MicroRNAs (miRNAs) in the C10orf116 overexpression 3T3-L1 adipocytes and performed target prediction and functional enrichment of the differentially expressed miRNAs. Our study identified 34 miRNAs up-regulated in the 3T3-L1 adipocytes stably overexpressing C10orf116, whereas 43 miRNAs up-regulated in the control cells. The target genes of differentially expressed miRNAs were found to be involved in multiple signalling pathways, such as Wnt, TGF-beta, MAPK, Jak-STAT, insulin signalling pathway, et al. Our data provided novel information for the identification of C10orf116.
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[Advances in research on antischistosomal drugs].
Zhongguo Xue Xi Chong Bing Fang Zhi Za Zhi
PUBLISHED: 12-15-2011
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The article summarizes the newest researches of antischistosomal drugs and discusses the possible alternatives to praziquantel from three aspects, so as to provide the evidence for the development of antischistosomal drugs.
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Brain-derived neurotrophic factor contributes to abdominal pain in irritable bowel syndrome.
Gut
PUBLISHED: 10-13-2011
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Brain-derived neurotrophic factor (BDNF), a member of the neurotrophin family, may play a critical role in many chronic pain conditions. The possible involvement of BDNF in the altered gut sensation in patients with irritable bowel syndrome (IBS) was investigated in the present study.
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Demonstration of a compact temperature sensor based on first-order Bragg grating in a tapered fiber probe.
Opt Express
PUBLISHED: 09-22-2011
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We experimentally demonstrate an all-silica first-order fiber Bragg grating (FBG) for high temperature sensing by focused ion beam (FIB) machining in a fiber probe tapered to a point. This 61-period FBG is compact (~36.6 ?m long and ~6.5 ?m in diameter) with 200-nm-deep shallow grooves. We have tested the sensor from room temperature to around 500 °C and it shows a temperature sensitivity of nearly 20 pm/°C near the resonant wavelength of 1550 nm. This kind of sensor takes up little space because of its unique geometry and small size and may be integrated in devices that work in harsh environment or for detecting small objects.
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Enhanced nucleation rate of polylactide in composites assisted by surface acid oxidized carbon nanotubes of different aspect ratios.
ACS Appl Mater Interfaces
PUBLISHED: 09-02-2011
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Biodegradable polylactide (PLA) composites added with acid oxidized multiwalled carbon nanotubes (A-MWCNTs) of two different aspect ratios (length to diameter) were prepared by coagulation. The aspect ratios and surface structures of A-MWCNTs were characterized by TGA, Raman, and SEM measurements. The percolation thresholds for gelation in the PLA composites with A-MWCNTs of large and small aspect ratios are 2.5 and 4.0 wt %, respectively, which were determined by a rheological method, and in turn, the rheological result confirms the aspect ratio differences for the added two types of A-MWCNTs in the composites. Isothermal crystallization kinetics of neat PLA and its composites were further investigated by using polarized optical microscope (POM) and differential scanning calorimetry (DSC) to clarify the effects of A-MWCNTs of different aspect ratios and concentrations. The different aspect ratio A-MWCNTs with the same carboxyl group mass percent show substantial effects on PLA crystallization kinetics. Those with smaller aspect ratios enhance nucleation rate for PLA spherulites much more than those with larger aspect ratios. This phenomenon can be attributed to fewer sidewall carboxyl groups on the surfaces of A-MWCNTs with smaller aspect ratios, which provides more nucleation sites for PLA crystallization than those with larger aspect ratios at the same concentration, resulting in faster PLA nucleation rates for the former one.
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Miniature tapered photonic crystal fiber interferometer with enhanced sensitivity by acid microdroplets etching.
Appl Opt
PUBLISHED: 08-12-2011
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We fabricate a miniature tapered photonic crystal fiber (PCF) interferometer with enhanced sensitivity by acid microdroplets etching. This method is very simple and cost effective, avoiding elongating the PCF, moving and refixing the device during etching, and measuring. The refractive index sensing properties with different PCF diameters are investigated both theoretically and experimentally. The tapering velocity can be controlled by the microdroplet size and position. The sensitivity greatly increases (five times, 750?nm/RIU) and the size decreases after slightly tapering the PCF. The device keeps low temperature dependence before and after tapering. More uniformly and thinly tapered PCFs can be realized with higher sensitivity (?100 times) by optimizing the etching process.
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KIR and HLA loci are associated with hepatocellular carcinoma development in patients with hepatitis B virus infection: a case-control study.
PLoS ONE
PUBLISHED: 05-25-2011
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Natural killer (NK) cells activation has been reported to contribute to inflammation and liver injury during hepatitis B virus (HBV) infection both in transgenic mice and in patients. However, the role of NK cells in the process of HBV-associated hepatocellular carcinoma (HCC) development has not been addressed. Killer cell immunoglobulin-like receptors (KIRs) are involved in regulating NK cell activation through recognition of specific human leukocyte antigen (HLA) class I allotypes.
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Complex nanoscale cage clusters built from uranyl polyhedra and phosphate tetrahedra.
Inorg Chem
PUBLISHED: 05-19-2011
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Five cage clusters that self-assemble in alkaline aqueous solution have been isolated and characterized. Each is built from uranyl hexagonal bipyramids with two or three equatorial edges occupied by peroxide, and three also contain phosphate tetrahedra. These clusters contain 30 uranyl polyhedra; 30 uranyl polyhedra and six pyrophosphate groups; 30 uranyl polyhedra, 12 pyrophosphate groups, and one phosphate tetrahedron; 42 uranyl polyhedra; and 40 uranyl polyhedra and three pyrophosphate groups. These clusters present complex topologies as well as a range of compositions, sizes, and charges. Two adopt fullerene topologies, and the others contain combinations of topological squares, pentagons, and hexagons. An analysis of possible topologies further indicates that higher-symmetry topologies are favored.
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Body mass index, recreational physical activity and female urinary incontinence in Gansu, China.
Eur. J. Obstet. Gynecol. Reprod. Biol.
PUBLISHED: 02-15-2011
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To elucidate the influence of recreational physical activity, body mass index (BMI), and waist circumference on the risk of specific types of urinary incontinence.
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Knockdown of STEAP4 inhibits insulin-stimulated glucose transport and GLUT4 translocation via attenuated phosphorylation of Akt, independent of the effects of EEA1.
Mol Med Rep
PUBLISHED: 02-11-2011
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The aim of this study was to investigate whether the early endosome antigen 1 (EEA1) and/or PI3K pathway is involved in the molecular mechanisms underlying the effects of the six-transmembrane protein of prostate 4 (STEAP4; also called STAMP2 and TIARP) on the insulin sensitivity of human adipocytes. Our data demonstrated that siRNA-mediated STEAP4 deficiency significantly decreased insulin-stimulated glucose transport in mature human adipocytes by decreasing GLUT4 translocation to the plasma membrane through attenuated Akt phosphorylation. We further found that EEA1 may not be involved in the mechanisms underlying the effects of STEAP4 on insulin-stimulated glucose uptake and GLUT4 translocation, as indicated by the results that i) STEAP4 does not alter the effects of EEA1 on insulin-stimulated glucose uptake and GLUT4 translocation; ii) STEAP4 does not modify the expression of EEA1 protein; and iii) STEAP4 does not interact with EEA1 according to FRET analysis. In conclusion, this study revealed that the knockdown of STEAP4 inhibits insulin-stimulated glucose transport and GLUT4 translocation via the attenuated phosphorylation of Akt, independent of the effects of EEA1.
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The psychometric properties of the Chinese version of the Problem Areas in Psychological Empowerment Scale (PES): scale development.
J Clin Nurs
PUBLISHED: 01-12-2011
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This study was conducted to adapt the Problem Areas in Psychological Empowerment Scale for use among staff nurses and to evaluate the psychometric properties.
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Feasible temperature of percutaneous microwave ablation of dog liver abutting the bowel.
Int J Hyperthermia
PUBLISHED: 01-04-2011
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This study used a dog model to determine the optimal temperature of percutaneous microwave ablation that causes complete necrosis of liver but not the adjacent bowel, supporting the use of this method to specifically and effectively treat liver tumour abutting the bowel.
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Uranium pyrophosphate/methylenediphosphonate polyoxometalate cage clusters.
J. Am. Chem. Soc.
PUBLISHED: 09-04-2010
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Despite potential applications in advanced nuclear energy systems, nanoscale control of uranium materials is in its infancy. In its hexavalent state, U occurs as (UO(2))(2+) uranyl ions that are coordinated by various ligands to give square, pentagonal, or hexagonal bipyramids. Creation and design of nanostructured uranyl materials requires interruption of the tendency of uranyl bipyramids to share equatorial edges to form infinite sheets that occur in extended structures. Where a bidentate peroxide group bridges uranyl bipyramids, the configuration is inherently bent, fostering formation of cage clusters. Here the bent configurations of four- and five-membered rings of uranyl peroxide hexagonal bipyramids are bridged by pyrophosphate or methylenediphosphonate, creating eight chemically complex cage clusters with specific topologies. Chemical complexity in such clusters provides opportunities for the tuning of cage sizes, pore sizes, and properties such as aqueous solubility. Several of these are topological derivatives of simpler clusters that contain only uranyl bipyramids, whereas others exhibit new topologies.
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Prevalence of irritable bowel syndrome in Chinese college and university students assessed using Rome III criteria.
World J. Gastroenterol.
PUBLISHED: 09-01-2010
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To estimate the prevalence of irritable bowel syndrome (IBS) in college and university students of North China and certain related factors for IBS.
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The molecular mechanism of protective effects of grape seed proanthocyanidin extract on reperfusion arrhythmias in rats in vivo.
Biol. Pharm. Bull.
PUBLISHED: 05-13-2010
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Reperfusion arrhythmias (RA) especially ventricular tachycardia (VT) and ventricular fibrillation (VF) remain the most important causes of sudden death following reperfusion. In isolated rat hearts grape seed proanthocyanidin extract (GSPE) had been proved to reduce the incidence of reperfusion-induced VF and VT. However the mechanism of this protection remained unclear. The aim of this study was to elucidate the potential mechanism of this protection of GSPE. The myocardial ischemia reperfusion (IR) model was induced by 30 min coronary occlusion and 120 min reperfusion in open chest anesthetized rats. The ultrastructure of ischemic cardiomyocytes was observed. An isobaric tag labeling for relative and absolute quantification (iTRAQ) proteomics was used to identify differentially expressed membrane proteins. Western blot was performed to verify the results of iTRAQ. The results demonstrated GSPE can significantly reduce the incidence of VT and VF induced by reperfusion in vivo. We identified 92 differentially expressed proteins. Western blot analysis confirmed GSPE increased the expression of Na(+)/K(+)-ATPase alpha1 subunit (p<0.01). We found the subunit distribution of Na(+)/K(+)-ATPase was changed after reperfusion. Na(+)/K(+)-ATPase alpha1 subunit was decreased in IR group (without GSPE-treated) compared to sham group while it was significantly increased in GSPE group. The decrease of free radical generation induced by GSPE may lead to the up-regulation of Na(+)/K(+)-ATPase alpha1 subunit. This change of subunit distribution may lead to the increase of activity of Na(+)/K(+)-ATPase which may result in the protection of GSPE against reperfusion arrhythmias. Our experiments provided new avenues for the treatment of reperfusion arrhythmias.
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[Clinical results of ultrasound biometry in silicone oil-filled eye].
Zhonghua Yan Ke Za Zhi
PUBLISHED: 04-15-2010
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To evaluate a method of ultrasound biometry in silicone oil-filled eye and its clinical results.
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[The disruptive effect of NN-methylene-bis on the function of FRTL-5 cells].
Sichuan Da Xue Xue Bao Yi Xue Ban
PUBLISHED: 04-08-2010
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To study the impact of N N-methylene-bis on thyroglobulin produced by FRTL-5 cells, and to explore the potential of using FRTL-5 cells to screen environmental thyroid hormone disruptors in vitro.
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Gene expression profiles of adipose tissue of high-fat diet-induced obese rats by cDNA microarrays.
Mol. Biol. Rep.
PUBLISHED: 02-17-2010
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To better understand the molecular basis of dietary obesity, we examined adipose tissue genes differentially expressed in a well-characterized rat model of high-fat diet (HFD)-induced obesity using cDNA microarrays. Male Sprague-Dawley rats were fed either the HFD or the normal diet. Seven weeks later, the weights of obese models (362.92 ± 39.65 g) were significantly higher than those of normal control rats (315.22 ± 42.30 g, P < 0.01) and the wet weights of adipose tissue of rats fed with HFD (9.29 ± 5.14 g) were significantly higher than those of normal control rats (4.09 ± 2.69 g, P < 0.01), which confirmed the successful preparation of obese models. cDNA microarrays containing 9 216 genes/Ests were used to investigate gene expression of adipose tissue. Autoradiographic analysis showed that 532, 154, and 22 genes were differently expressed over 2-, 3-, and 5-fold, respectively. The analysis of gene expression profiles indicated that 276 genes were up-regulated and 432 genes were down-regulated in response to HFD-induced obesity. Different clusters of genes associated with lipid metabolism, extracellular matrix, signal transduction, cytoskeleton, cell apoptosis, etc., such as VLCS-H2, DGAT, ACADVL, PHYH, SCD, ACACA, ACS, MMP-2, MMP-15, CD38, CAMK2D, CACNA1F, CAPZA2, TMOD3, ARPC2, KNS2, TPM1, MAPK8, GADD45B, DAXX, TOK-1, PRKACA, STAT6, were concerned.
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[Resistin inhibits rat insulinoma cell RINm5F proliferation].
Zhongguo Dang Dai Er Ke Za Zhi
PUBLISHED: 02-02-2010
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Resistin was thought to link the obesity to type 2 diabetes. This study aimed to investigate the effect of resistin on insulinoma cell proliferation.
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Optimization of medium for one-step fermentation of inulin extract from Jerusalem artichoke tubers using Paenibacillus polymyxa ZJ-9 to produce R,R-2,3-butanediol.
Bioresour. Technol.
PUBLISHED: 01-20-2010
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The medium for one-step fermentation of raw inulin extract from Jerusalem artichoke tubers by Paenibacillus polymyxa ZJ-9 to produce R,R-2,3-butanediol (R,R-2,3-BD) was developed. Inulin, K(2)HPO(4) and NH(4)Cl were found to be the key factors in the fermentation according to the results obtained from the Plackett-Burman experimental design. The optimal concentration range of the three factors was examined by the steepest ascent path, and their optimal concentration was further investigated according to the Box-Behnken design and determined to be 77.14 g/L, 3.09 g/L and 0.93 g/L, respectively. Under the optimal conditions, the concentration of the obtained R,R-2,3-BD was 36.92 g/L, at more than 98% optical purity. Compared with other investigated carbon resources, fermentation of the raw inulin extract afforded the highest yield of R,R-2,3-BD. This process featured one-step fermentation of inulin without further hydrolyzing, which greatly decreased the raw material cost and thus facilitated its practical application.
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[Determination of plasma protein binding rate of tetramethylpyrazine phosphate by ultrafiltration].
Zhong Yao Cai
PUBLISHED: 01-07-2010
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To study the plasma protein binding rate of Tetramethylpyrazine Phosphate.
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[Effects of NYGGF4 gene over-expression on the insulin sensitivity and secretory function of adipocytes].
Zhongguo Dang Dai Er Ke Za Zhi
PUBLISHED: 10-24-2009
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To study the effect of a new obesity-related gene NYGGF4 on the insulin sensitivity and secretory function of adipocytes.
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[Construction of eukaryotic expression vector of LYRM1, a novel gene related with human obesity, and establishment of its stable transfected 3T3-L1 cell line].
Zhejiang Da Xue Xue Bao Yi Xue Ban
PUBLISHED: 10-16-2009
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To construct the eukaryotic expression vector of LYRM1 and to transfect it to preadipocytes cell line 3T3-L1.
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Resistin induces rat insulinoma cell RINm5F apoptosis.
Mol. Biol. Rep.
PUBLISHED: 10-02-2009
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Beta-cell apoptosis induced by adipokines may result in beta-cell dysfunction in type 2 diabetes. Resistin, an adipokine-linked obesity with type 2 diabetes, impairs glucose-stimulated insulin secretion (GSIS) in beta-cells. Presently, the effects of resistin on rat insulinoma cells RINm5F were examined. Treatment of RINm5F with resistin induced cell damage. Tissue Inhibitor of Metalloproteinase-1 (TIMP-1) protected resistin-mediated cytotoxicity in RINm5F. Incubation with resistin up-regulated caspase-3 activity and induced the formation of a DNA ladder. TIMP-1 attenuated these effects. The molecular mechanism of TIMP-1 inhibition of resistin-mediated cytotoxicity appeared to involve Akt phosphorylation and activation of IkB-alpha phosphorylation. Resistin treatment suppressed Akt phosphorylation and activated IkB-alpha phosphorylation, which could be attenuated by TIMP-1. We conclude that resistin can induce beta-cell apoptosis and that resistin-related beta-cell apoptosis can be prevented by TIMP-1.
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[Effect of initial substrate concentrations and pH on hydrogen production from xylose with Clostridium butyricum T4].
Sheng Wu Gong Cheng Xue Bao
PUBLISHED: 09-26-2009
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We studied the effect of initial pH and substrate concentrations on the conversion of xylose to hydrogen by Clostridium butyrium T4 at pH 5.0-8.5 and substrate concentrations 5-40 g/L. The cumulative hydrogen volume and the specific hydrogen production rate increased and then decreased with increasing initial pH or substrate concentrations. At initial pH 6.5 and substrate concentration 20 g/L, the cumulative hydrogen production and the specific hydrogen production rate reached the maximum value of 4.26 L/L and 18.86 mmol-H2/h g-DCW (dry cell weight).
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Protective effect of grape seed proanthocyanidins extracts on reperfusion arrhythmia in rabbits.
J. Nutr. Sci. Vitaminol.
PUBLISHED: 07-16-2009
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Reperfusion arrhythmia (RA) is one of the main complications which are also an important cause of sudden cardiac death. The aim of this study was to clarify whether grape seed proanthocyanidins extracts (GSPE) were therapeutic agents against RA. The models of cardiac ischemic reperfusion injury were established in rabbits. GSPE (100 mg/kg, and 250 mg/kg body weight/d, respectively) were administered for 3 wk. The incidence rates of arrhythmias before and after reperfusion of each group were recorded, cardiac infarction area and microstructures of cardiac cells of each rabbit were observed, and the expression of connexin 43 (Cx43) was detected by immunohistochemistry. Data were analyzed using the Leica Qwin V3 image analysis system. Reperfusion induced arrhythmia. Ventricular fibrillation (VF) occurred during the early phase of reperfusion after ischemia. Our results showed that GSPE treatment significantly reduced the incidence of VF and the infarction size compared with the model control group. Moreover, the intercalated disks in the model control group showed collapse, displacement and even the formation of cisterns. After being treated by GSPE, the intercalated disks were improved and there were less collapse and displacement. The expression of Cx43 was improved by GSPE treatment, and high dose of GSPE resulted in significant improvement. The study suggests that GSPE has a protective effect on myocardial ischemic reperfusion arrhythmias, which may be mediated by inhibiting the degradation of Cx43 and enhancing gap junctional conductance.
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[Urethral guidance probe applied to surgical urethral realignment].
Zhonghua Nan Ke Xue
PUBLISHED: 07-15-2009
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To search for a safe and convenient surgical method for management of urethral disruption.
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Surgery for thoracic myelopathy caused by ossification of the ligamentum flavum.
J Clin Neurosci
PUBLISHED: 06-28-2009
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Between January 1996 and December 2003, our department treated 16 patients (10 men and 6 women; average age 57.5 years) by performing a laminectomy for thoracic myelopathy caused by ossification of the ligamentum flavum (OLF). We followed up all patients for 36 to 86 months (mean follow-up time, 57.3 months). The mean (+/-standard deviation) Japanese Orthopaedic Association score increased from 5.0+/-1.4 points before the operation to 7.7+/-1.9 points at the last follow-up (p<0.01). The average values for pre-operative and post-operative kyphosis of the involved vertebrae were 5.8 degrees +/-4.1 degrees and 8.8 degrees +/-6.0 degrees , respectively; the mean increase in kyphosis was only 3.0 degrees +/-2.4 degrees . An intraoperative dural tear was the main complication and none of the patients developed severe neurological complications. We conclude that laminectomy was both effective and safe in the treatment of thoracic OLF, but it must be performed with great care because of frequent dural adhesions to the OLF. The increase in kyphosis after the laminectomy was minimal when most of the facet joints were left intact and when the patient followed a back extensor exercise program post-operatively.
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[Comparison of indirect immunofluorescence assay and ELISA for detecting antinuclear antibodies and anti-double-stranded DNA antibodies].
Nan Fang Yi Ke Da Xue Xue Bao
PUBLISHED: 03-24-2009
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To compare indirect immunofluorescence assay (IIFA) and enzyme-linked immunosorbent assay (ELISA) for detecting antinuclear antibodies (ANA) and anti-double-stranded DNA antibodies (anti-dsDNA).
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LYRM1, a novel gene promotes proliferation and inhibits apoptosis of preadipocytes.
Eur. J. Endocrinol.
PUBLISHED: 03-05-2009
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To characterize a novel gene, Homo sapiens LYR motif containing 1 (LYRM1), that is highly expressed in omental adipose tissue of obese subjects.
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Gene expression profiles in the prefrontal cortex of SHR rats by cDNA microarrays.
Mol. Biol. Rep.
PUBLISHED: 01-07-2009
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We have undertaken cDNA microarrays to identify differentially expressed genes in the prefrontal cortex (PFC) of spontaneously hypertensive-rat (SHR), a rodent model of attention deficit hyperactivity disorder (ADHD) versus control Wistar-Kyoto (WKY) rats. The analysis of the gene expression profiles indicated that 57 genes were up-regulated and 97 genes were down-regulated in the PFC of SHR. These predominately expressed genes included genes involved in neural development, immunity, transcription factor, monoamine neurotransmitter, metabolism, signal transduction, apoptosis and so on. Although more detailed analyses are necessary, it is anticipated that further study of genes identified will provide insights into their specific roles in the etiology of ADHD.
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[Effects of revegetation on organic carbon storage in deep soils in hilly Loess Plateau region of Northwest China].
Ying Yong Sheng Tai Xue Bao
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Taking the Robinia pseudoacacia woodlands, Caragana korshinskii shrublands, and abandoned croplands with different years of revegetation in the hilly Loess Plateau region of Northwest China as test objects, this paper studied the profile distribution and accumulation dynamics of organic carbon storage in deep soil (100-400 cm), with those in 0-100 cm soil profile as the control. In 0-100 cm soil profile, the organic carbon storage decreased significantly with the increase of soil depth; while in deep soil, the organic carbon storage had a slight fluctuation. The total organic carbon storage in 100-400 cm soil profile was considerably high, accounting for approximately 60% of that in 0-400 cm soil profile. The organic carbon storage in 80-100 cm soil layer had a significant linear correlation with that in 100-200 and 200-400 cm soil layers, and among the organic carbon storages in the five layers in 0-100 cm soil profile, the organic carbon storage in 80-100 cm soil layer had the strongest correlation with that in 100-400 cm soil profile, being able to be used to estimate the organic carbon storage in deep soil in this region. The organic carbon storage in 0-20 cm soil layer in the three types of revegetation lands was significantly higher than that in slope croplands, but the organic carbon storage in deep soil had no significant difference among the land use types. The organic carbon storage in deep soil increased with the increasing years of revegetation. In R. pseudoacacia woodlands and C. korshinskii shrub lands, the average increasing rate of the organic carbon storage in 100-400 cm soil layer was 0.14 and 0.19 t x hm(-2) x a(-1), respectively, which was comparable to that in the 0-100 cm soil layer in C. korshinskii shrublands. It was suggested that in the estimation of the soil carbon sequestration effect of revegetation in hilly Loess Plateau region, the organic carbon accumulation in deep soil should be taken into consideration. Otherwise, the effect of revegetation on soil carbon sequestration would be significantly underestimated.
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