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Find video protocols related to scientific articles indexed in Pubmed.
Recruited Alveolar Macrophages, in Response to Airway Epithelial-derived MCP-1/CCL2, Regulate Airway Inflammation and Remodeling in Allergic Asthma.
Am. J. Respir. Cell Mol. Biol.
PUBLISHED: 11-01-2014
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Rationale: Although alveolar macrophages (AM) from asthmatics are known to be functionally different than those of healthy individuals, the mechanism by which this transformation occurs has not been fully elucidated in asthma. Objective: To define the mechanism(s) that controls AM phenotypic and functional transformation in response to acute allergic airway inflammation. Methods: The phenotype and functional characteristics of AM obtained from human asthmatics following subsegmental bronchoprovocation with allergen (SBP-AG) was studied. Using macrophage-depleted mice, the role and trafficking of AM populations was interrogated using an acute allergic lung inflammation model. Measurements and Main Results: We observed that depletion of AMs in a mouse allergic asthma model attenuates Th2-type allergic lung inflammation and its consequent airway remodeling. In both human and mouse, endobronchial challenge with allergen induced a marked increase in macrophage chemotactic proteins (MCPs) in BAL fluid concomitant with the rapid appearance of a monocyte-derived population of AM. Furthermore, airway allergen challenge of allergic subjects with mild asthma skewed the pattern of AM gene expression toward high levels of the receptor for MCP1 (CCR2/MCP1R) and expression of M2 phenotypic proteins, whereas most pro-inflammatory genes were highly suppressed. Interestingly, CCL2/MCP-1 gene expression was prominent in bronchial epithelial cells in a mouse allergic asthma model, and in vitro studies indicate that bronchial epithelial cells produced abundant MCP-1 in response to house dust mite allergen. Thus, our study indicates that bronchial allergen challenge induces the recruitment of blood monocytes along a chemotactic gradient generated by allergen-exposed bronchial epithelial cells.
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[Effect of lipopolysaccharide on the expression of ALP,BSP,DSPP in rat dental pulp cells].
Shanghai Kou Qiang Yi Xue
PUBLISHED: 10-24-2014
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To study the changes of expression of dentin sialophosphoprotein (DSPP), bone sialoprotein (BSP) and alkaline phosphatase (ALP) in rat dental pulp cells, which were treated with lipopolysaccharide (LPS).
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[Effect of acupuncture intervention on gastrointestinal motility and liver pathological changes in cirrhotic rats].
Zhen Ci Yan Jiu
PUBLISHED: 09-16-2014
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To observe the effect of acupuncture intervention on gastrointestinal motility and liver pathologic changes in cirrhotic rats so as to reveal its underlying mechanisms in improving cirrhosis.
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Relationship Between Procalcitonin Serum Levels and Functional Outcome in Stroke Patients.
Cell. Mol. Neurobiol.
PUBLISHED: 09-10-2014
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To determine whether serum procalcitonin (PCT) levels at admission were associated with short-term functional outcome after acute ischemic stroke (AIS) in a cohort Chinese sample. We prospectively studied 378 patients with AIS who were admitted within 24 h after the onset of symptoms. PCT and NIH stroke scale (NIHSS) were measured at the time of admission. Short-term functional outcome was measured by modified Rankin scale (mRS) 90 days after admission. The results indicated that the serum PCT levels were significantly higher in AIS patients as compared to normal controls (P < 0.0001). In the 114 patients with an unfavorable functional outcome, serum PCT levels were higher compared with those in patients with a favorable outcome (2.40 (IQR, 1.10-3.69) ng/mL and 0.42 (IQR, 0.10-1.05) ng/mL, respectively, P < 0.001). PCT was an independent prognostic marker of functional outcome [odds ratio (OR) 3.45 (2.29-4.77), adjusted for the NIHSS and other possible confounders] in patients with ischemic stroke, added significant additional predictive value to the clinical NIHSS score. In receiver operating characteristic curve analysis, the prognostic accuracy of PCT was higher compared to Hs-CRP and NIHSS score. PCT is an independent predictor of short-term functional outcome after ischemic stroke in Chinese sample even after correcting for possible confounding factors.
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Development of Second-Generation Small-Molecule RhoA Inhibitors with Enhanced Water Solubility, Tissue Potency, and Significant in vivo Efficacy.
ChemMedChem
PUBLISHED: 09-08-2014
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RhoA, a member of the Rho GTPases, is involved in a variety of cellular functions and could be a suitable therapeutic target for the treatment of cardiovascular diseases. However, few small-molecule RhoA inhibitors have been reported. Based on our previously reported lead compounds, 32 new 2-substituted quinoline (or quinoxaline) derivatives were synthesized and tested in biological assays. Six compounds showed high RhoA inhibitory activities, with IC50 values of 1.17-1.84??M. Among these, (E)-3-(3-(ethyl(quinolin-2-yl)amino)phenyl)acrylic acid (26?b) and (E)-3-(3-(butyl(quinolin-2-yl)amino)phenyl)acrylic acid (26?d) demonstrated noticeable vasorelaxation effects against phenylephrine-induced contraction in thoracic aorta artery rings, and compound 26?b had good water solubility and showed significant in vivo efficacy, which was similar to that of 5-(1,4-diazepane-1-sulfonyl)isoquinoline (fasudil) in a subarachnoid hemorrhage-cardiovascular model. To the best of our knowledge, compound 26?b is the first example of a small- molecule RhoA inhibitor with potent in vivo efficacy, which could serve as a good lead for designing cardiovascular agents.
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[Mangiferin protects rats against chronic bronchitis via regulating NF-kappaB (P65) and IkappaBalpha expression in mononuclear cells].
Yao Xue Xue Bao
PUBLISHED: 08-26-2014
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This study is to investigate the protective effect of mangiferin on NF-kappaB (P65) and IkappaBalpha expression in peripheral blood mononuclear cell (PBMC) in rats with cigarette smoke induced chronic bronchitis. The rat model with chronic bronchitis was established by cigarette smoke. Real-time fluorescence RT-PCR was executed for evaluating the NF-kappaB (P65) and IKkappaBalpha gene expression in mononuclear cell, and flow cytometry for their protein expression. The serum hs-CRP (high-sensitivity C-reactive proteins) and TNF-alpha (tumor necrosis factor-alpha) were detected by enzyme-linked immunosorbent assay. The histopathological score was obtained from lung tissue HE staining slides of lung tissue. The results showed that mangiferin could markedly suppress the NF-kappaB (P65) mRNA and protein expression in mononuclear cell, while promote the IkappaBalpha mRNA and protein expression. Furthermore, mangiferin could lower serum hs-CRP and TNF-alpha level, and reduce the chronic inflammatory damage of bronchiole. These results suggested that mangiferin could notably ameliorate chronic bronchiole inflammation induced by cigarette smoke, and this protective effect might be linked to the regulation of NF-kappaB (P65) and IkappaBalpha expression in mononuclear cell.
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Iminoxyl radical-promoted dichotomous cyclizations: efficient oxyoximation and aminooximation of alkenes.
Org. Lett.
PUBLISHED: 08-25-2014
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A novel iminoxyl radical-involved metal-free approach to vicinal oxyoximation and aminooximation of unactivated alkenes is developed. This method utilizes the dichotomous reactivity of the iminoxyl radical to furnish a general difunctionalization on alkenes using simple tert-butyl nitrite (TBN) as the iminoxyl radical initiator as well the carbon radical trap. By using this protocol, oxime featured 4,5-dihydroisoxazoles and cyclic nitrones were facilely prepared from ?,?- and ?,?-unsaturated ketoximes, respectively.
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Beta-2 adrenergic receptor (ADRB2) gene polymorphisms and the risk of asthma: a meta-analysis of case-control studies.
PLoS ONE
PUBLISHED: 08-11-2014
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A number of studies have assessed the relationship between beta-2 adrenergic receptor (ADRB2) gene polymorphisms and asthma risk. However, the results are inconsistent. A meta-analysis that focused on the association between asthma and all ADRB2 polymorphisms with at least three case-control studies was thus performed.
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Complete mitochondrial genome sequence of the blue sheep, Pseudois nayaur szechuanensis (Cetartiodactyla: Caprinae).
Mitochondrial DNA
PUBLISHED: 08-05-2014
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Abstract The blue sheep (Pseudois nayaur szechuanensis) belongs to the subfamily Caprinae, which distributes in Sichuan, Gansu, Qinghai, southeastern Xinjiang, northern Yunnan and Ningxia-Inner Mongolia border, China. In this study, the complete mitochondrial genome of P. n. szechuanensis was sequenced. The mitogenome was 16,738?bp in length, consisting of 13 protein-coding genes, 22 transfer RNA (tRNA) genes, 2 ribosomal RNA (rRNA) genes, and a non-coding control region. As in other mammals, most mitochondrial genes are encoded on the heavy strand, except for ND6 and eight tRNA genes which are encoded on the light strand. The overall base composition of the P. n. szechuanensis is 33.5% A, 26.4% T, 27.0% C, and 13.2% G. The alignment of the Pseudois species control regions exhibited high genetic variability and rich A?+?T content. The complete mitogenome of P. n. szechuanensis can provide an important data for the studies on phylogenetic relationship and population genetics to further explore the taxonomic status of this species.
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Combined effects methylation of FHIT, RASSF1A and RAR? genes on non-small cell lung cancer in the Chinese population.
Asian Pac. J. Cancer Prev.
PUBLISHED: 07-22-2014
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Epigenetic modifications of tumour suppressor genes are involved in all kinds of human cancer. Aberrant promoter methylation is also considered to play an essential role in development of lung cancer, but the pathogenesis remains unclear.We collected the data of 112 subjects, including 56 diagnosed patients with lung cancer and 56 controls without cancer. Methylation of the FHIT, RASSF1A and RAR-? genes in DNA from all samples and the corresponding gene methylation status were assessed using the methylation-specific polymerase chain reaction (PCR, MSP). The results showed that the total frequency of separate gene methylation was significantly higher in lung cancer compared with controls (33.9-85.7 vs 0 %) (p<0.01).Similar outcomes were obtained from the aberrant methylation of combinations of any two or three genes (p<0.01). There was a tendency that the frequency of combinations of any two or three genes was higher in stage I+II than that in stage III+IV with lung cancer. However, no significant difference was found across various clinical stages and clinic pathological gradings of lung cancer (p>0.05).These observations suggest that there is a significant association of promoter methylation of individual genes with lung cancer risk, and that aberrant methylation of combination of any two or three genes may be associated with clinical stage in lung cancer patients and involved in the initiation of lung cancer tumorigenesis. Methylation of FHIT, RASSF1A and RAR? genes may be related to progression of lung oncogenesis.
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Clostridium difficile carriage in hospitalized cancer patients: a prospective investigation in eastern China.
BMC Infect. Dis.
PUBLISHED: 07-16-2014
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Clostridium difficile carriage has been considered as a potential source for the deadly infection, but its role in cancer patients is still unclear. We aimed to identify the clinical and immunological factors that are related to C. difficile carriage in Chinese cancer patients.
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The role of preoperative neutrophil-lymphocyte and platelet-lymphocyte ratio in patients after radical resection for gastric cancer.
Biomarkers
PUBLISHED: 06-09-2014
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The objective of this study was to investigate the impact of neutrophil-lymphocyte ratio (NLR) and the platelet-lymphocyte ratio (PLR) on the postoperative complication and long-term outcomes in patients with resectable gastric cancer (GC). A total of 377 patients who underwent curative resection for GC were enrolled. In logistic analysis, PLR (p?=?0.09) was independently associated with the incidence of postoperative complication. The results of multivariate survival analysis showed the NLR and PLR were introduced as prognostic factors for operable GC, the NLR may represent a useful prognostic index for the prediction of overall survival (OS) in advanced GC (p?=?0.021).
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Incorporation of perineural invasion of gastric carcinoma into the 7th edition tumor-node-metastasis staging system.
Tumour Biol.
PUBLISHED: 05-15-2014
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The aim of this study was to determine the prognostic value of perineural invasion (PNI) in patients with gastric cancer who underwent curative resection. We retrospectively analyzed 518 patients who had undergone curative gastrectomy. Paraffin sections of surgical specimens from all patients were stained with hematoxylin and eosin. PNI was defined when carcinoma cells infiltrated into the perineurium or neural fascicles. Patients with PNI had a significantly larger tumors (?5.0 cm), lymphatic venous invasion (positive), deeper tumor invasion (T4), more number of lymph node metastases (N3), and higher tumor stage (III). Regarding survival, multivariate analysis showed that PNI emerged as an independent prognostic factor for survival (hazard ratio (HR)?=?1.901, P?
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Chlorination and chloramination of tetracycline antibiotics: disinfection by-products formation and influential factors.
Ecotoxicol. Environ. Saf.
PUBLISHED: 05-08-2014
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Formation of disinfection by-products (DBPs) from chlorination and chloramination of tetracycline antibiotics (TCs) was comprehensively investigated. It was demonstrated that a connection existed between the transformation of TCs and the formation of chloroform (CHCl3), carbon tetrachloride (CCl4), dichloroacetonitrile (DCAN) and dichloroacetone (DCAce). Factors evaluated included chlorine (Cl2) and chloramine(NH2Cl) dosage, reaction time, solution pH and disinfection modes. Increased Cl2/NH2Cl dosage and reaction time improved the formation of CHCl3 and DCAce. Formation of DCAN followed an increasing and then decreasing pattern with increasing Cl2 dosage and prolonged reaction time. pH affected DBPs formation differently, with CHCl3 and DCAN decreasing in chlorination, and having maximum concentrations at pH 7 in chloramination. The total concentrations of DBPs obeyed the following order: chlorination>chloramination>pre-chlorination (0.5h)>pre-chlorination (1h)>pre-chlorination (2h).
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Preventing catheter-related bacteremia with taurolidine-citrate catheter locks: a systematic review and meta-analysis.
Blood Purif.
PUBLISHED: 04-26-2014
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Preliminary evidence from some studies suggests that taurolidine-citrate locks decrease catheter-related bacteremia (CRB), which is a major cause of morbidity and mortality in patients using intravascular catheters. No previous study has sought to summarize existing evidence on the use of taurolidine-citrate locks. A systematic review and meta-analysis were undertaken to determine whether taurolidine-citrate was more effective than heparin in the prevention of CRB.
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Sequential treatment of icotinib after first-line pemetrexed in advanced lung adenocarcinoma with unknown EGFR gene status.
J Thorac Dis
PUBLISHED: 04-12-2014
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In non-small cell lung cancer (NSCLC), the well-developed epidermal growth factor receptor (EGFR) is an important therapeutic target. EGFR activating gene mutations have been proved strongly predictive of response to EGFR-tyrosine kinase inhibitors (TKI) in NSCLC. However, both in daily clinical practice and clinical trials, patients with unknown EGFR gene status (UN-EGFR-GS) are very common. In this study, we assessed efficacy and tolerability of sequential treatment of first-line pemetrexed followed by icotinib in Chinese advanced lung adenocarcinoma with UN-EGFR-GS.
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Prognostic value of number of examined lymph nodes in patients with node-negative gastric cancer.
World J. Gastroenterol.
PUBLISHED: 04-08-2014
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To elucidate the potential impact of examined lymph nodes (eLNs) on long-term survival of node-negative gastric cancer patients after curative surgery.
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The Transcription Factor Nuclear Factor of Activated T Cells c3 Modulates the Function of Macrophages in Sepsis.
J Innate Immun
PUBLISHED: 04-03-2014
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The role of the transcription factor nuclear factor of activated T cells (NFAT) was initially identified in T and B cell gene expression, but its role in regulating gene expression in macrophages during sepsis is not known. Our data show that NFATc3 regulates expression of inducible nitric oxide synthase (iNOS) in macrophages stimulated with lipopolysaccharide. Selective inhibition of NFAT by cyclosporine A and a competitive peptide inhibitor 11R-VIVIT inhibited endotoxin-induced expression of iNOS and nitric oxide (NO) release. Macrophages from NFATc3 knockout (KO) mice show reduced iNOS expression and NO release and attenuated bactericidal activity. Gel shift and chromatin immunoprecipitation assays show that endotoxin challenge increases NFATc3 binding to the iNOS promoter, resulting in transcriptional activation of iNOS. The binding of NFATc3 to the iNOS promoter is abolished by NFAT inhibitors. NFATc3 KO mice subjected to sepsis show that NFATc3 is necessary for bacterial clearance in mouse lungs during sepsis. Our study demonstrates for the first time that NFATc3 is necessary for macrophage iNOS expression during sepsis, which is essential for containment of bacterial infections. © 2014 S. Karger AG, Basel.
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Penicillium marneffei infection within an osteolytic lesion in an HIV-negative patient.
Int. J. Infect. Dis.
PUBLISHED: 03-19-2014
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Penicillium marneffei is a thermally dimorphic pathogenic fungus that causes systemic infection similar to disseminated cryptococcosis. P. marneffei is endemic in Southeast Asia, usually infecting HIV-infected individuals; infection of HIV-negative individuals is extremely rare. Here, we describe a disseminated P. marneffei infection within an osteolytic lesion in an HIV-negative patient. A 40-year-old Chinese woman presented with intermittent fever, generalized lymphadenopathy, and a skin rash. Following a sternum biopsy, the patient was diagnosed with P. marneffei infection. An emission computed tomography bone scan revealed the presence of increased radioactivity in the left clavicle and sternum, indicative of an osteolytic lesion. In addition to reporting this very rare case, we also present a brief review of the literature, highlighting the differences in clinical manifestations between HIV-positive and HIV-negative patients infected with P. marneffei as it applies to our case.
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Prognostic nutritional index predicts postoperative complications and long-term outcomes of gastric cancer.
World J. Gastroenterol.
PUBLISHED: 03-12-2014
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To investigate the impact of prognostic nutritional index (PNI) on the postoperative complications and long-term outcomes in gastric cancer patients undergoing total gastrectomy.
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Radical induced degradation of acetaminophen with Fe3O4 magnetic nanoparticles as heterogeneous activator of peroxymonosulfate.
J. Hazard. Mater.
PUBLISHED: 03-12-2014
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Magnetic nano-scaled particles Fe3O4 were studied for the activation of peroxymonosulfate (PMS) to generate active radicals for degradation of acetaminophen (APAP) in water. The Fe3O4 MNPs were found to effectively catalyze PMS for removal of APAP, and the reactions well followed a pseudo-first-order kinetics pattern (R(2)>0.95). Within 120min, approximately 75% of 10ppm APAP was accomplished by 0.2mM PMS in the presence of 0.8g/L Fe3O4 MNPs with little Fe(3+) leaching (<4?g/L). Higher Fe3O4 MNP dose, lower initial APAP concentration, neutral pH, and higher reaction temperature favored the APAP degradation. The production of sulfate radicals and hydroxyl radicals was validated through two ways: (1) indirectly from the scavenging tests with scavenging agents, tert-butyl alcohol (TBA) and ethanol (EtOH); (2) directly from the electron paramagnetic resonance (ESR) tests with 0.1M 5,5-dimethyl-1-pyrrolidine N-oxide (DMPO). Plausible mechanisms on the radical generation from Fe3O4 MNP activation of PMS are proposed based on the results of radical identification tests and XPS analysis. It appeared that Fe(2+)Fe(3+) on the catalyst surface was responsible for the radical generation. The results demonstrated that Fe3O4 MNPs activated PMS is a promising technology for water pollution caused by contaminants such as pharmaceuticals.
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Catgut implantation at acupoints for allergic rhinitis: a systematic review.
Chin J Integr Med
PUBLISHED: 03-04-2014
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To assess the effectiveness and the possible adverse effects of catgut implantation at acupoints for allergic rhinitis (AR).
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Node-extranodal soft tissue stage based on extranodal metastasis is associated with poor prognosis of patients with gastric cancer.
J. Surg. Res.
PUBLISHED: 02-21-2014
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The purpose of this study was to evaluate the effect of Node-Extranodal soft tissue (pNE) stage based on Extranodal Metastasis (EM) on recurrence and survival in patients with gastric cancer (GC).
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Effect of complication grade on survival following curative gastrectomy for carcinoma.
World J. Gastroenterol.
PUBLISHED: 02-10-2014
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To elucidate the potential impact of the grade of complications on long-term survival of gastric cancer patients after curative surgery.
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APC(Cdc20) suppresses apoptosis through targeting Bim for ubiquitination and destruction.
Dev. Cell
PUBLISHED: 02-08-2014
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Anaphase-promoting complex Cdc20 (APC(Cdc20)) plays pivotal roles in governing mitotic progression. By suppressing APC(Cdc20), antimitotic agents activate the spindle-assembly checkpoint and induce apoptosis after prolonged treatment, whereas depleting endogenous Cdc20 suppresses tumorigenesis in part by triggering mitotic arrest and subsequent apoptosis. However, the molecular mechanism(s) underlying apoptosis induced by Cdc20 abrogation remains poorly understood. Here, we report the BH3-only proapoptotic protein Bim as an APC(Cdc20) target, such that depletion of Cdc20 sensitizes cells to apoptotic stimuli. Strikingly, Cdc20 and multiple APC-core components were identified in a small interfering RNA screen that, upon knockdown, sensitizes otherwise resistant cancer cells to chemoradiation in a Bim-dependent manner. Consistently, human adult T cell leukemia cells that acquire elevated APC(Cdc20) activity via expressing the Tax viral oncoprotein exhibit reduced Bim levels and resistance to anticancer agents. These results reveal an important role for APC(Cdc20) in governing apoptosis, strengthening the rationale for developing specific Cdc20 inhibitors as effective anticancer agents.
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Experimental research on the relationship between fit accuracy and fracture resistance of zirconia abutments.
J Dent
PUBLISHED: 02-05-2014
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The purpose of the study was to investigate the correlation between fit accuracy and fracture resistance of zirconia abutments, as well as its feasibility for clinical applications.
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Effect of chlorine dioxide on cyanobacterial cell integrity, toxin degradation and disinfection by-product formation.
Sci. Total Environ.
PUBLISHED: 01-31-2014
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Bench scale tests were conducted to study the effect of chlorine dioxide (ClO2) oxidation on cell integrity, toxin degradation and disinfection by-product formation of Microcystis aeruginosa. The simulated cyanobacterial suspension was prepared at a concentration of 1.0×10(6)cells/mL and the cell integrity was measured with flow cytometry. Results indicated that ClO2 can inhibit the photosynthetic capacity of M. aeruginosa cells and almost no integral cells were left after oxidation at a ClO2 dose of 1.0mg/L. The total toxin was degraded more rapidly with the ClO2 dosage increasing from 0.1mg/L to 1.0mg/L. Moreover, the damage on cell structure after oxidation resulted in released intracellular organic matter, which contributed to the formation of trihalomethanes (THMs) and haloacetic acids (HAAs) as disinfection by-products. Therefore, the use of ClO2 as an oxidant for treating algal-rich water should be carefully considered.
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Impact of passive smoking, cooking with solid fuel exposure, and MBL/MASP-2 gene polymorphism upon susceptibility to tuberculosis.
Int. J. Infect. Dis.
PUBLISHED: 01-26-2014
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To explore the impact of passive smoking, cooking with solid fuel, mannose-binding lectin (MBL) gene, MBL-associated serine proteases 2 (MASP-2) gene, and gene-environment interactions on the susceptibility to tuberculosis (TB) in non-smokers.
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Identification and catalytic characterization of a nonribosomal peptide synthetase-like (NRPS-like) enzyme involved in the biosynthesis of echosides from Streptomyces sp. LZ35.
Gene
PUBLISHED: 01-25-2014
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Echosides, isolated from Streptomyces sp. LZ35, represent a class of para-terphenyl natural products that display DNA topoisomerase I and II? inhibitory activities. By analyzing the genome draft of strain LZ35, the ech gene cluster was identified to be responsible for the biosynthesis of echosides, which was further confirmed by gene disruption and HPLC analysis. Meanwhile, the biosynthetic pathway for echosides was proposed. Furthermore, the echA-gene, encoding a tri-domain nonribosomal peptide synthetase (NRPS)-like enzyme, was identified as a polyporic acid synthetase and biochemically characterized in vitro. This is the first study to our knowledge on the biochemical characterization of an Actinobacteria quinone synthetase, which accepts phenylpyruvic acid as a native substrate. Therefore, our results may help investigate the function of other NRPS-like enzymes in Actinobacteria.
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A study of weekly docetaxel and carboplatin as first-line chemotherapy for advanced non-small cell lung cancer.
J Thorac Dis
PUBLISHED: 01-22-2014
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Weekly docetaxel demonstrated similar efficacy but better tolerability than standard triweekly docetaxel, and carboplatin was less nephrotoxic, neurotoxic and emetogenic than cisplatin. This study aimed to evaluate the efficacy and safety of weekly docetaxel with carboplatin as first-line chemotherapy for advanced non-small cell lung cancer (NSCLC).
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Clinical significance of lymph node metastasis in gastric cancer.
World J. Gastroenterol.
PUBLISHED: 01-03-2014
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Gastric cancer, one of the most common malignancies in the world, frequently reveals lymph node, peritoneum, and liver metastases. Most of gastric cancer patients present with lymph node metastasis when they were initially diagnosed or underwent surgical resection, which results in poor prognosis. Both the depth of tumor invasion and lymph node involvement are considered as the most important prognostic predictors of gastric cancer. Although extended lymphadenectomy was not considered a survival benefit procedure and was reported to be associated with high mortality and morbidity in two randomized controlled European trials, it showed significant superiority in terms of lower locoregional recurrence and disease related deaths compared to limited lymphadenectomy in a 15-year follow-up study. Almost all clinical investigators have reached a consensus that the predictive efficiency of the number of metastatic lymph nodes is far better than the extent of lymph node metastasis for the prognosis of gastric cancer worldwide, but other nodal metastatic classifications of gastric cancer have been proposed as alternatives to the number of metastatic lymph nodes for improving the predictive efficiency for patient prognosis. It is still controversial over whether the ratio between metastatic and examined lymph nodes is superior to the number of metastatic lymph nodes in prognostic evaluation of gastric cancer. Besides, the negative lymph node count has been increasingly recognized to be an important factor significantly associated with prognosis of gastric cancer.
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Systemically transplanted human gingiva-derived mesenchymal stem cells contributing to bone tissue regeneration.
Int J Clin Exp Pathol
PUBLISHED: 01-01-2014
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As novel postnatal stem cells, gingiva-derived mesenchymal stem cells (GMSCs) have been considered as an ideal candidate cell resource for tissue engineering and cell-based therapies. GMSCs implanted into sites of injury have been confirmed to promote the injury repair. However, no studies have demonstrated whether systemically transplanted GMSCs can home to the bone injuries and contribute to the new bone formation in vivo. In this study, we transplanted human GMSCs into C57BL/6J mice with defects in mandibular bone via the tail vein to explore the capacity of transplanted GMSCs to promote bone regeneration. Results showed that the transplanted GMSCs were detected in the bone defects and employed in new bone formation. And the newly formed bone area in mice with GMSCs transplantation was significantly higher than that in control mice. Our findings indicate that systemically transplanted GMSCs can not only home to the mandibular defect but also promote bone regeneration.
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Epithelioid malignant mesothelioma presenting with features of gastric tumor in a child.
Int J Clin Exp Pathol
PUBLISHED: 01-01-2014
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Localized malignant mesothelioma is very uncommon and mainly arises in pleura and peritoneum, and preferentially occurs in older adults. In this article, we report a case of a Localized malignant mesothelioma that was developed in the stomach of a 6-year-old boy. This boy was admitted to hospital for anemia. An epigastric mass was palpated through systemic physical examination and MR scanning demonstrated an 8×6 cm-sized, well-defined elliptic mass at gastric corpus. Partial resection of the stomach was performed for this boy and no nodules were found on the liver, peritoneum, and other abdominal sites in surgery. In view of the morphological and immunohistochemical findings, a diagnosis of localized malignant mesothelioma, epithelial type was made. This is the first case report of localized malignant mesothelioma arising in the stomach of a child. Accumulation of more cases of malignant mesothelioma involving gastrointestinal tract and longer follow-up of the patients are necessary to further characterize the features of this rare disease.
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Cuevaenes C-E: Three new triene carboxylic derivatives from Streptomyces sp. LZ35?gdmAI.
Beilstein J Org Chem
PUBLISHED: 01-01-2014
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Two pairs of geometrical isomers - cuevaenes A (1) and C (3) as well as cuevaenes D (4) and E (5) - and cuevaene B (2) were isolated from gdmAI-disrupted Streptomyces sp. LZ35. The constitution of cuevaene C (3) was found to be identical to cuevaene A (1) by means of NMR spectroscopy and high resolution mass spectrometry. However, the relative configurations of the triene side chain moieties were determined to be different. It was established on the basis of spectroscopic data that cuevaenes D (4) and E (5) are amides and geometrical isomers. Cuevaenes A-C (1-3) displayed moderate activity against Gram-positive bacteria (e.g., Bacillus subtilis strain ATCC 11060) and modest activity against fungi (e.g., Fusarium verticillioides strain S68 and Rhizoctonia solani strain GXE4). However, cuevaenes D (4) and E (5) showed no inhibitory activity against any of the tested microbes.
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Krüppel like factor 4 promoter undergoes active demethylation during monocyte/macrophage differentiation.
PLoS ONE
PUBLISHED: 01-01-2014
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The role of different lineage specific transcription factors in directing hematopoietic cell fate towards myeloid lineage is well established but the status of epigenetic modifications has not been defined during this important developmental process. We used non proliferating, PU.1 inducible myeloid progenitor cells and differentiating bone marrow derived macrophages to study the PU.1 dependent KLF4 transcriptional regulation and its promoter demethylation during monocyte/macrophage differentiation. Expression of KLF4 was regulated by active demethylation of its promoter and PU.1 specifically bound to KLF4 promoter oligo harboring the PU.1 consensus sequence. Methylation specific quantitative PCR and Bisulfite sequencing indicated demethylation of CpG residues most proximal to the transcription start site of KLF4 promoter. Cloned KLF4 promoter in pGL3 Luciferase and CpG free pcpgf-bas vectors showed accentuated reporter activity when co-transfected with the PU.1 expression vector. In vitro methylation of both KLF4 promoter oligo and cloned KLF4 promoter vectors showed attenuated in vitro DNA binding activity and Luciferase/mouse Alkaline phosphotase reporter activity indicating the negative influence of KLF4 promoter methylation on PU.1 binding. The Cytosine deaminase, Activation Induced Cytidine Deaminase (AICDA) was found to be critical for KLF4 promoter demethylation. More importantly, knock down of AICDA resulted in blockade of KLF4 promoter demethylation, decreased F4/80 expression and other phenotypic characters of macrophage differentiation. Our data proves that AICDA mediated active demethylation of the KLF4 promoter is necessary for transcriptional regulation of KLF4 by PU.1 during monocyte/macrophage differentiation.
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Prognostic value of perineural invasion in gastric cancer: a systematic review and meta-analysis.
PLoS ONE
PUBLISHED: 01-01-2014
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The prognostic role of perineural invasion in gastric cancer is controversial. Here, we present a systemic review and meta-analysis of the association between perineural invasion and survival in resectable gastric cancer patients.
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Characterization of algal organic matters of Microcystis aeruginosa: biodegradability, DBP formation and membrane fouling potential.
Water Res.
PUBLISHED: 01-01-2014
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Algal organic matters (AOM), including extracellular organic matters (EOM) and intracellular organic matters (IOM), were comprehensively studied in terms of their biodegradability, disinfection byproduct (DBP) formation potentials and membrane fouling. EOM and IOM were fractionated into hydrophobic (HP), transphilic (TP) and hydrophilic (HL) constituents. The HP, TP and HL fractions of EOM and IOM were highly biodegradable with BDOC/DOC ranging from 52.5% to 67.4% and the DBP formation potentials followed the order of HP > TP > HL, except of IOM-HL. Biodegradable process proved very effective in removing the DBP formation potentials. Moreover, the AOM characteristics were also evaluated during ultrafiltration (UF) treatment. Results demonstrated that UF favourably remove DOC and DBP formation potential of IOM than those of EOM. And the HL constituents played a more important role in membrane fouling than HP and TP. The UF foulants exhibited higher BDOC/DOC than AOM, suggesting EOM and IOM might enhance biofouling because more biodegradable proteins and polysaccharides were found in membrane foulants. Therefore, appropriate biological treatment, ultrafiltration, or combination of the both are potential options to address these algae-caused water quality issues.
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Priming BCL-2 to kill: the combination therapy of tamoxifen and ABT-199 in ER+ breast cancer.
Breast Cancer Res.
PUBLISHED: 10-31-2013
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The B-cell lymphoma/leukemia 2 protein (BCL-2) may help many types of cancers to evade cell death. However, identifying exactly where this is the case is a challenge. ABT-199 is a small molecule that selectively inhibits BCL-2, which is currently in clinical trials in lymphoid malignancies. While inhibiting BCL-2 by itself can cause cell death in hematopoietic tumors, single-agent activity is harder to observe in solid tumors. Combining ABT-199 with tamoxifen, the standard endocrine therapy for estrogen receptor-positive breast cancers, 85% of which have BCL-2 expression, represents a new strategy to prime cancer cells for apoptosis and elicit better cancer cell death responses.
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Fluorescence Linked Enzyme Chemoproteomic Strategy for Discovery of a Potent and Selective DAPK1 and ZIPK Inhibitor.
ACS Chem. Biol.
PUBLISHED: 10-17-2013
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DAPK1 and ZIPK (also called DAPK3) are closely related serine/threonine protein kinases that regulate programmed cell death and phosphorylation of non-muscle and smooth muscle myosin. We have developed a fluorescence linked enzyme chemoproteomic strategy (FLECS) for the rapid identification of inhibitors for any element of the purinome and identified a selective pyrazolo[3,4-d]pyrimidinone (HS38) that inhibits DAPK1 and ZIPK in an ATP-competitive manner at nanomolar concentrations. In cellular studies, HS38 decreased RLC20 phosphorylation. In ex vivo studies, HS38 decreased contractile force generated in mouse aorta, rabbit ileum, and calyculin A stimulated arterial muscle by decreasing RLC20 and MYPT1 phosphorylation. The inhibitor also promoted relaxation in Ca(2+)-sensitized vessels. A close structural analogue (HS43) with 5-fold lower affinity for ZIPK produced no effect on cells or tissues. These findings are consistent with a mechanism of action wherein HS38 specifically targets ZIPK in smooth muscle. The discovery of HS38 provides a lead scaffold for the development of therapeutic agents for smooth muscle related disorders and a chemical means to probe the function of DAPK1 and ZIPK across species.
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Epidemiology of methicillin-resistant Staphylococcus aureus infection and empirical antibiotic therapy for MRSA infection: multicenter investigation.
Chin. Med. J.
PUBLISHED: 10-12-2013
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The epidemiology of methicillin-resistant Staphylococcus aureus (MRSA) maybe changed by strict infection control measures, and the impact of empirical antibiotic therapy on the outcomes of MRSA infection was not clear. We aimed to investigate the present epidemiological status of MRSA infection and empirical antibiotic therapy for MRSA infection in university teaching hospitals in mainland China.
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[Effect of infiltration technique and polishing on the roughness of artificial carious enamel surfaces].
Shanghai Kou Qiang Yi Xue
PUBLISHED: 10-09-2013
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To study the surface roughness of early carious lesions which were treated with resin infiltration and polished with different materials, and to provide reference for selection of appropriate polishing system.
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[Effect of N-acetyl-seryl-aspartyl-lysyl-proline on differentiation from pulmonary fibroblast to myofibroblast mediated by Rho-associated coiled-coilforming protein kinase pathway].
Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi
PUBLISHED: 09-26-2013
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To investigate whether N-acetyl-seryl-aspartyl-lysyl-proline (Ac-SDKP) can inhibit the differentiation of pulmonary fibroblasts into myofibroblasts by regulating Rho-associated coiled-coil forming protein kinase (ROCK) pathway mediated by transforming growth factor-?1 (TGF-?1).
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[The hot point and controversy of clinical measures in critical are medicine].
Sichuan Da Xue Xue Bao Yi Xue Ban
PUBLISHED: 09-25-2013
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With its powerful ability to monitoring and support of organs, the survival rate of critically ill patients has been improved significantly by critical care medicine. Critical ill patients centralized in ICU accepted the treatments given by multidisciplinary team as a mode have played a very important role, especially during the rescue of victims in public health emergencies and natural disasters. Changings of the measures of monitoring and support are always along with the development of the critical care medicine. Fluid therapy is the most core measures in ICU, but there is a significant contradiction between positive fluid therapy and patients impaired organ compensatory ability. The premise of appropriate fluid therapy is continuous and accurate evaluation of the hemodynamic status of critical ill patients. The variation of peak velocity of common carotid artery measured by bedside ultrasound can estimate the volume status of the SICU patients accurately. This method has high feasibility and accuracy and easy to be handled by the residents of ICU. The non-C. difficile associated nosocomial diarrhea even fatal enteritis in ICU can be caused by Klebsiella oxytoca and Clostridium perfringens. Although this kind of incidence occurs in a relatively low rate, it always associates with severe diseases and poor outcome. Analgesia and sedation had become routine measures in ICU. There is still great controversy in some of the sedation protocol, such as sedation level and wake up daily. More and more evidence-based data suggested taking appropriate sedation targeting to individuals, and the protocol should be integrated with the dealing of pain, anxiety and delirium.
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Autotaxin production of lysophosphatidic acid mediates allergic asthmatic inflammation.
Am. J. Respir. Crit. Care Med.
PUBLISHED: 09-21-2013
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Bioactive lipid mediators, derived from membrane lipid precursors, are released into the airway and airspace where they bind high-affinity cognate receptors and may mediate asthma pathogenesis. Lysophosphatidic acid (LPA), a bioactive lipid mediator generated by the enzymatic activity of extracellular autotaxin (ATX), binds LPA receptors, resulting in an array of biological actions on cell proliferation, migration, survival, differentiation, and motility, and therefore could mediate asthma pathogenesis.
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Analysis of start-up, retention, and adherence in ALS clinical trials.
Neurology
PUBLISHED: 09-04-2013
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To investigate predictors of trial start-up times, high attrition, and poor protocol adherence in amyotrophic lateral sclerosis (ALS) trials.
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[Efficacy controlled observation on acupuncture and western medicine for gastrointestinal dysmotility in liver cirrhosis].
Zhongguo Zhen Jiu
PUBLISHED: 07-27-2013
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To observe the therapeutic effect of acupuncture on gastrointestinal dysmotility in liver cirrhosis.
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[The significance of No.13 lymph node dissection in D2 gastrectomy for lower-third advanced gastric cancer].
Zhonghua Wai Ke Za Zhi
PUBLISHED: 07-18-2013
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To evaluate the feasibility and necessity of No.13 lymph node dissection in D2 radical gastrectomy for lower-third advanced gastric cancer (AGC).
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[The prognostic analysis of tumor size in T4a stage gastric cancer].
Zhonghua Wai Ke Za Zhi
PUBLISHED: 07-18-2013
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To investigate the impact of tumor size in the prognosis of T4a stage gastric cancer.
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Influence of hydrophobic/hydrophilic fractions of extracellular organic matters of Microcystis aeruginosa on ultrafiltration membrane fouling.
Sci. Total Environ.
PUBLISHED: 06-29-2013
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Fouling is a major obstacle to maintain the efficiency of ultrafiltration-based drinking water treatment process. Algal extracellular organic matters (EOMs) are currently considered as one of the major sources of membrane fouling. The objective of this study was to investigate the influence of different hydrophobic/hydrophilic fractions of EOM extracted from Microcystis aeruginosa on ultrafiltration membrane fouling at lab scale. The experimental data indicated that EOM exhibited similar flux decline trends on polyethersulfone (PES) and regenerated cellulose (RC) membranes but caused greater irreversible fouling on PES membrane than RC membrane due to its hydrophobic property. It was also observed that charged hydrophilic (CHPI) and neutral hydrophilic (NHPI) fractions caused greater flux decline over hydrophobic (HPO) and transphilic (TPI) fractions. For PES membrane, the order of the irreversible fouling potentials for the four fractions was HPO>TPI>CHPI>NHPI, while the irreversible fouling potentials of RC membrane were tiny and could be ignored. Fluorescence excitation-emission matrix (EEM) spectra and Fourier transform infrared (FTIR) spectra suggested that protein-like, polysaccharide-like and humic-like substances were the major components responsible for membrane fouling. The results also indicated that the irreversible fouling increased as the pH decreased. The addition of calcium to feed solutions led to more severe flux decline and irreversible fouling.
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Dendrimer-encapsulated silver nanoparticles and antibacterial activity on cotton fabric.
J Nanosci Nanotechnol
PUBLISHED: 06-13-2013
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Silver-dendrimer nanocomposites are synthesized in the presence low generation poly(amido amine) dendrimers with terminal amine groups employing silver nitrate as the precursor and sodium borohydride as the reduction agent. The parameters including the addition of oxalic acid and the generation of the dendrimer that influence the particle size of the synthesized silver nanoparticles are discussed. Under the optimized conditions, a series of silver-dendrimer nanocomposites with the average diameter of silver nanoparticles ranging from 7.6 nm to 16.2 nm are synthesized by varying the molar ratio of dendrimer to silver nitrate. The synthesized silver-dendrimer nanocomposites are utilized as the antibacterial agents in fabrication of antibacterial cotton fabrics. The antimicrobial properties of the prepared antibacterial cotton fabrics against E. coli and S. aureus are evaluated by the inhibition zone method. The results demonstrate that the antimicrobial activity of the silver-dendrimer composites treated cotton fabric enhances with the decrease of the silver nanoparticle size.
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Studies on CD36 deficiency in South China: Two cases demonstrating the clinical impact of anti-CD36 antibodies.
Thromb. Haemost.
PUBLISHED: 06-06-2013
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CD36 (also known as GPIV) deficiency is known to be responsible for the production of anti-Naka antibodies in different clinical settings such as fetal/neonatal alloimmune thrombocytopenia (FNAIT), platelet transfusion refractoriness (PTR) and post-transfusion purpura (PTP). However, no data regarding the relevance of CD36 immunisation is currently available for China. In this study, healthy blood donors were typed for CD36 deficiency using flow cytometry. Nucleotide sequencing was performed to identify the molecular basis underlying the CD36 deficiency. Anti-Naka antibodies in CD36-deficient individuals were analysed by ELISA and flow cytometry. By analysis of 998 healthy blood donors, 18 individuals failed to express CD36 on their platelets. In 5/12 individuals no CD36 expression was detected both on platelets and monocytes. This result suggested that the frequencies of type I CD36 deficiency (platelets and monocytes) and type II CD36 deficiency (platelets only) are approximately 0.5 and 1.3%, respectively. Nucleotide sequencing analysis of type I CD36 deficient individuals revealed eight different mutations; four of them were not described so far. However, 1228-1239del ATTGTGCCTATT and 329-330delAC appear to be the most common mutations related to type I CD36 deficiency in South Chinese population. Further analysis showed that 1/5 type I CD36 deficient individuals developed anti-Naka antibodies. In addition, anti-Naka antibodies could be identified in two cases of thrombocytopenia associated with FNAIT and PTR. In conclusion, more than 0.5% of CD36 type I-deficient individuals are at risk to be immunised through blood transfusion or pregnancy in China. Testing of anti-Naka antibodies should be considered in FNAIT and PTR suspected cases. A registry of CD36-deficient donors should be established to allow treatment of immune-mediated bleeding disorders caused by anti-Naka antibodies.
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Prevalence of ocular manifestations of HIV/AIDS in the highly active antiretroviral therapy (HAART) era: a different spectrum in Central South China.
Ophthalmic Epidemiol
PUBLISHED: 05-30-2013
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To investigate ocular manifestations of human immunodeficiency virus/acquired immunodeficiency syndrome (HIV/AIDS) in a population in central south China during a time of highly active antiretroviral therapy (HAART).
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Effects of extended lymphadenectomy and postoperative chemotherapy on node-negative gastric cancer.
World J. Gastroenterol.
PUBLISHED: 05-21-2013
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To investigate the effects of extended lymphadenectomy and postoperative chemotherapy on gastric cancer without lymph node metastasis.
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Characteristics and prognosis of gastric cancer in patients aged ? 70 years.
World J. Gastroenterol.
PUBLISHED: 05-19-2013
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To elucidate the prognostic value of age for gastric cancer and identify the optimal treatment for elderly gastric cancer patients.
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Impact of intraoperative blood loss on survival after curative resection for gastric cancer.
World J. Gastroenterol.
PUBLISHED: 05-16-2013
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To elucidate the potential impact of intraoperative blood loss (IBL) on long-term survival of gastric cancer patients after curative surgery.
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[The initial CT findings in patients suffering from invasive pulmonary aspergillosis].
Zhonghua Wei Zhong Bing Ji Jiu Yi Xue
PUBLISHED: 05-11-2013
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To approach the initial CT findings of invasive pulmonary aspergillosis (IPA) in patients with immunosuppression.
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CoFe2O4 magnetic nanoparticles as a highly active heterogeneous catalyst of oxone for the degradation of diclofenac in water.
J. Hazard. Mater.
PUBLISHED: 05-02-2013
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A magnetic nanoscaled catalyst cobalt ferrite (CoFe2O4) was successfully prepared and used for the activation of oxone to generate sulfate radicals for the degradation of diclofenac. The catalyst was characterized by transmission electron microscopy, X-ray diffractometry, Fourier transform infrared spectroscopy and vibrating sample magnetometer. The effects of calcination temperature, initial pH, catalyst and oxone dosage on the degradation efficiency were investigated. Results demonstrated that CoFe2O4-300 exhibited the best catalytic performance and almost complete removal of diclofenac was obtained in 15 min. The degradation efficiency increased with initial pH decreasing in the pH range of 5-9. The increase of catalyst and oxone dosage both had the positive effect on the degradation of diclofenac. Moreover, CoFe2O4 could retain high degradation efficiency even after being reused for five cycles. Finally, the major diclofenac degradation intermediates were identified and the primary degradation pathways were proposed.
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[Clinicopathological characteristics and prognostic analysis of bone metastasis in gastric cancer patients].
Zhonghua Wei Chang Wai Ke Za Zhi
PUBLISHED: 04-24-2013
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To investigate the clinicopathological characteristics and prognostic factors of bone metastasis in gastric cancer patients.
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Real-time 3D ultrasound imaging of infant tongue movements during breast-feeding.
Early Hum. Dev.
PUBLISHED: 04-18-2013
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Whether infants use suction or peristaltic tongue movements or a combination to extract milk during breast-feeding is controversial. The aims of this pilot study were 1] to evaluate the feasibility of using 3D ultrasound scanning to visualise infant tongue movements; and 2] to ascertain whether peristaltic tongue movements could be demonstrated during breast-feeding.
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[The value of negative lymph node count in prediction of prognosis of advanced gastric cancer].
Zhonghua Wai Ke Za Zhi
PUBLISHED: 04-13-2013
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To evaluate the value of negative lymph node count (NLNC) in prediction of prognosis of advanced gastric cancer after radical resection.
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[Analysis of clinicopathologic characteristics and prognosis on mixed histology type of gastric cancer].
Zhonghua Wei Chang Wai Ke Za Zhi
PUBLISHED: 03-29-2013
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To evaluate the clinicopathologic characteristics and prognosis of mixed histological type(MHT)gastric cancer.
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Emodin improves lipopolysaccharide-induced microcirculatory disturbance in rat mesentery.
Microcirculation
PUBLISHED: 03-27-2013
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Sepsis is a systemic inflammatory response syndrome. Emodin is a major ingredient of Rheum Palmatum, a Chinese herb that is widely used in China for treatment of endotoxemia-related diseases. This study intended to examine the effect of Emodin on LPS-induced rat mesenteric microcirculatory disturbance and the underlying mechanisms.
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Degradation of antipyrine by UV, UV/H?O? and UV/PS.
J. Hazard. Mater.
PUBLISHED: 03-21-2013
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Degradation of antipyrine (AP) in water by three UV-based photolysis processes (i.e., direct UV, UV/H?O?, UV/persulfate (UV/PS)) was studied. For all the oxidation processes, the AP decomposition exhibited a pseudo-first-order kinetics pattern. Generally, UV/H?O? and UV/PS significantly improved the degradation rate relevant to UV treatment alone. The pseudo-first-order degradation rate constants (kobs) were, to different degrees, affected by initial AP concentration, oxidant dose, pH, UV irradiation intensity, and co-existing chemicals such as humic acid, chloride, bicarbonate, carbonate and nitrate. The three oxidation processes followed the order in terms of treatment costs: UV/PS>UV>UV/H?O? if the energy and chemical costs are considered. Finally, the AP degradation pathways in the UV/H?O? and UV/PS processes are proposed. Results demonstrated that UV/H?O? and UV/PS are potential alternatives to control water pollution caused by emerging contaminants such as AP.
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[Prognostic value of metastatic lymph node ratio for gastric cancer patients with less than 15 lymph nodes dissection].
Zhonghua Wei Chang Wai Ke Za Zhi
PUBLISHED: 03-01-2013
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To evaluate the prognostic value of metastatic lymph node ratio (MLR) for gastric cancer patients with less than 15 lymph nodes dissected.
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Study on discrimination of white tea and albino tea based on near-infrared spectroscopy and chemometrics.
J. Sci. Food Agric.
PUBLISHED: 02-27-2013
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White tea and albino tea have their own nutritional characteristics, but from the appearance they are quite similar to each other. It is not easy to distinguish them with existing analytical tools or by visual inspection. The current study proposed a rapid method to discriminate them based on near-infrared (NIR) spectroscopy associated with supervised pattern recognition methods.
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Bare-metal stent versus drug-eluting stent in large coronary arteries: meta-analysis of randomized controlled trials.
Catheter Cardiovasc Interv
PUBLISHED: 02-26-2013
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Uncertainties exist with regard to the efficacy of drug-eluting stent (DES) versus bare-metal stent (BMS) in large coronary arteries.
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The morbidity rate of chronic disease among Chinese rural residents: results from Liuyang cohort.
Med Princ Pract
PUBLISHED: 01-25-2013
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To study the health status and explore the putative risk factors of chronic diseases among a Chinese rural population.
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Effects of different algaecides on the photosynthetic capacity, cell integrity and microcystin-LR release of Microcystis aeruginosa.
Sci. Total Environ.
PUBLISHED: 01-18-2013
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Bench scale tests were conducted to study the effects of four common algaecides, including copper sulfate, hydrogen peroxide, diuron and ethyl 2-methylacetoacetate (EMA) on the photosynthetic capacity, cell integrity and microcystin-LR (MC-LR) release of Microcystis aeruginosa. The release of potassium (K(+)) from cell membrane during algaecide exposure was also analyzed. The three typical photosynthetic parameters, including the effective quantum yield (?e), photosynthetic efficiency (?) and maximal electron transport rate (rETRmax), were measured by a pulse amplitude modulated (PAM) fluorometry. Results showed that the photosynthetic capacity was all inhibited by the four algaecides, to different degrees, by limiting the energy capture in photosynthesis, and blocking the electron transfer chain in primary reaction. For example, at high diuron concentration (7.5 mg L(-1)), ?e, ? and rETRmax decreased from 0.46 to 0.19 (p<0.01), from 0.20 to 0.01 (p<0.01) ?mol electrons m(-2) s(-1)/?mol photons m(-2) s(-1), and from 160.7 to 0.1 (p<0.001) ?mol m(-2) s(-1) compared with the control group after 96 h of exposure, respectively. Furthermore, the increase of algaecide dose could lead to the cell lysis, as well as release of intracellular MC-LR that enhanced the accumulation of extracellular MC-LR. The order of MC-LR release potential for the four algaecides was CuSO4>H2O2>diuron>EMA.
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Light propagation in a turbid medium with insonified microbubbles.
J Biomed Opt
PUBLISHED: 01-08-2013
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Surfactant stabilized microbubbles are widely used clinical contrast agents for ultrasound imaging. In this work, the light propagation through a turbid medium in the presence of microbubbles has been investigated. Through a series of experiments, it has been found that the optical attenuation is increased when the microbubbles in a turbid medium are insonified by ultrasound. Such microbubble enhanced optical attenuation is a function of both applied ultrasound pressure and microbubble concentration. To understand the mechanisms involved, a Monte Carlo (MC) model has been developed. Under ultrasound exposure, the sizes of microbubbles vary in space and time, and their dynamics are modeled by the Rayleigh-Plesset equation. By using Mie theory, the spatially and temporally varying optical scattering and scattering efficiency of microbubbles are determined based on the bubble sizes and internal refractive indices. The MC model is shown to effectively describe a medium with rapidly changing optical scattering, and the results are validated against both computational results using an N-layered diffusion equation model and experimental results using a clinical microbubble contrast agent (SonoVue®).
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JoVE Visualize is a tool created to match the last 5 years of PubMed publications to methods in JoVE's video library.

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In developing our video relationships, we compare around 5 million PubMed articles to our library of over 4,500 methods videos. In some cases the language used in the PubMed abstracts makes matching that content to a JoVE video difficult. In other cases, there happens not to be any content in our video library that is relevant to the topic of a given abstract. In these cases, our algorithms are trying their best to display videos with relevant content, which can sometimes result in matched videos with only a slight relation.