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Find video protocols related to scientific articles indexed in Pubmed.
Species-specific Exon Loss in Human Transcriptomes.
Mol. Biol. Evol.
PUBLISHED: 11-16-2014
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Changes in exon-intron structures and splicing patterns represent an important mechanism for the evolution of gene functions and species-specific regulatory networks. While exon creation is widespread during primate and human evolution and has been studied extensively, much less is known about the scope and potential impact of human-specific exon loss events. Historically, transcriptome data and exon annotations are significantly biased towards humans over nonhuman primates. This ascertainment bias makes it challenging to discover human-specific exon loss events. We carried out a transcriptome-wide search of human-specific exon loss events, by taking advantage of RNA-seq as a powerful and unbiased tool for exon discovery and annotation. Using RNA-seq data of humans, chimpanzees, and other primates, we reconstructed and compared transcript structures across the primate phylogeny. We discovered 33 candidate human-specific exon loss events, among which 6 exons passed stringent experimental filters for the complete loss of splicing activities in diverse human tissues. These events may result from human-specific deletion of genomic DNA, or small-scale sequence changes that inactivated splicing signals. The impact of human-specific exon loss events is predominantly regulatory. Three of the 6 events occurred in the 5'-UTR and affected cis regulatory elements of mRNA translation. In SLC7A6, a gene encoding an amino acid transporter, luciferase reporter assays showed that both a human-specific exon loss event and an independent human-specific single nucleotide substitution in the 5'-UTR increased mRNA translational efficiency. Our study provides novel insights into the molecular mechanisms and evolutionary consequences of exon loss during human evolution.
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Aberration correction of double-sided telecentric zoom lenses using lens modules.
Appl Opt
PUBLISHED: 10-17-2014
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A systematic approach for the aberration design of a four-component double-sided telecentric zoom lens system is presented. The Gaussian structure of the zoom system is specified previously which means the powers and movements of components are known. Each component is treated as a lens module during the design stage with specified first-order properties and third-order aberration targets. The third-order aberration targets of the first component are determined by minimizing the whole aberrations of the zoom lens system using a genetic algorithm (GA). And the aberration targets of components behind are determined by reoptimization with already fixed structures of previous components. Mean pupil spherical aberration of every component in every zoom position is adopted in the objective function to control high-order aberrations. The thin lens structure of each component can be determined from their first-order properties and aberration targets. After lens thickening and reoptimization, the zoom lens system can finally be determined.
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In situ SERS detection of emulsifiers at lipid interfaces using label-free amphiphilic gold nanoparticles.
Analyst
PUBLISHED: 08-19-2014
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Herein, we fabricated amphiphilic gold nanoparticles (GNPs) that can self-assemble at oil-water interfaces. We applied those GNPs for in situ SERS detection of emulsifier molecules within the interfacial region of oil in water (O/W) emulsion systems.
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Paraxial analysis of double-sided telecentric zoom lenses with three components.
Appl Opt
PUBLISHED: 08-05-2014
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A general method for the calculation of paraxial design parameters of a double-sided telecentric zoom lens system with three components is given. Formulas that define the interval distances between components while zooming, the extremum of magnification, and the magnification of each component are derived. The kinetic property of the zoom system is also discussed. As a result of the study, a classification of three-component double-sided telecentric zoom lenses is given, which is based on the magnification, signs of component optical power, and the position of stop while zooming. Some numerical examples are given in the third section of the paper.
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Development paths of China's agricultural Pharmaceutical industry under Eco-agriculture background.
Pak J Pharm Sci
PUBLISHED: 07-13-2014
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Using pesticides has double effects. On one hand, it contributes to pests control and regulates the growth of crops; On the other hand, it does harm to the environment. To develop ecological agriculture should not only emphasize the output level of agriculture to pursuit of economic efficiency, but also need to keep the ecological environment protected and focus on the social benefits during the development of the industry. As a large agricultural country in the world, China is vigorously promoting the development of ecological agriculture, which is bound to put forward to developing the pesticide industry and green ecological development requirements to promote the transformation and upgrading of agricultural pharmaceutical industry. This paper discusses the mechanism of pesticide pollution on the ecological environment and analyzes China's agricultural problems in the pharmaceutical industry. Then study on the development of Chinese green pesticides and try to find the proper paths of agricultural pharmaceutical to achieve industrial upgrading.
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mRNA-Seq and MicroRNA-Seq Whole-Transcriptome Analyses of Rhesus Monkey Embryonic Stem Cell Neural Differentiation Revealed the Potential Regulators of Rosette Neural Stem Cells.
DNA Res.
PUBLISHED: 06-17-2014
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Rosette neural stem cells (R-NSCs) represent early stage of neural development and possess full neural differentiation and regionalization capacities. R-NSCs are considered as stem cells of neural lineage and have important implications in the study of neurogenesis and cell replacement therapy. However, the molecules regulating their functional properties remain largely unknown. Rhesus monkey is an ideal model to study human neural degenerative diseases and plays intermediate translational roles as therapeutic strategies evolved from rodent systems to human clinical applications. In this study, we derived R-NSCs from rhesus monkey embryonic stem cells (ESCs) and systematically investigated the unique expressions of mRNAs, microRNAs (miRNAs), and signalling pathways by genome-wide comparison of the mRNA and miRNA profilings of ESCs, R-NSCs at early (R-NSCP1) and late (R-NSCP6) passages, and neural progenitor cells. Apart from the R-NSCP1-specific protein-coding genes and miRNAs, we identified several pathways including Hedgehog and Wnt highly activated in R-NSCP1. The possible regulatory interactions among the miRNAs, protein-coding genes, and signalling pathways were proposed. Besides, many genes with alternative splicing switch were identified at R-NSCP1. These data provided valuable resource to understand the regulation of early neurogenesis and to better manipulate the R-NSCs for cell replacement therapy.
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Evaluation of surface-enhanced Raman scattering detection using a handheld and a bench-top Raman spectrometer: a comparative study.
Talanta
PUBLISHED: 05-21-2014
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Surface enhanced Raman scattering (SERS) detection using a handheld Raman spectrometer and a bench-top Raman spectrometer was systemically evaluated and compared in this study. Silver dendrites were used as the SERS substrate, and two pesticides, maneb and pyrrolidine dithiocarbamate-ammonium salt (PDCA) were used as the analytes. Capacity and performance were evaluated based on spectral resolution, signal variation, quantitative capacity, sensitivity, flexibility and intelligence for SERS detection. The results showed that the handheld Raman spectrometer had better data consistency, more accurate quantification capacity, as well as the capacity of on-site and intelligence for qualitative and semi-quantitative analysis. On the other hand, the bench-top Raman spectrometer showed about 10 times higher sensitivity, as well as flexibility for optimization of the SERS measurements under different parameters such as laser power output, collective time, and objective magnification. The study on the optimization of SERS measurements on a bench-top spectrometer provides a useful guide for designing a handheld Raman spectrometer, specifically for SERS detection. This evaluation can advance the application of a handheld Raman spectrometer for the on-site measurement of trace amounts of pesticides or other chemicals.
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Fabrication of lipophilic gold nanoparticles for studying lipids by surface enhanced Raman spectroscopy (SERS).
Analyst
PUBLISHED: 05-20-2014
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A simple fabrication method for preparing lipophilic gold nanoparticles (AuNPs) suitable for use as substrates in surface-enhanced Raman scattering (SERS) applications of lipids was developed. The AuNPs were shown to be dispersible within lipid phases, where they greatly increased the SERS signals of lipids.
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A strongly coupled graphene and FeNi double hydroxide hybrid as an excellent electrocatalyst for the oxygen evolution reaction.
Angew. Chem. Int. Ed. Engl.
PUBLISHED: 02-26-2014
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Cost-effective electrocatalysts for the oxygen evolution reaction (OER) are critical to energy conversion and storage processes. A novel strategy is used to synthesize a non-noble-metal-based electrocatalyst of the OER by finely combining layered FeNi double hydroxide that is catalytically active and electric conducting graphene sheets, taking advantage of the electrostatic attraction between the two positively charged nanosheets. The synergy between the catalytic activity of the double hydroxide and the enhanced electron transport arising from the graphene resulted in superior electrocatalytic properties of the FeNi-GO hybrids for the OER with overpotentials as low as 0.21?V, which was further reduced to 0.195?V after the reduction treatment. Moreover, the turnover frequency at the overpotential of 0.3?V has reached 1?s(-1), which is much higher than those previously reported for non-noble-metal-based electrocatalysts.
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Tangeretin-loaded protein nanoparticles fabricated from zein/?-lactoglobulin: preparation, characterization, and functional performance.
Food Chem
PUBLISHED: 02-19-2014
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The aim of this study was to design a colloidal delivery system to encapsulate poor water-soluble bioactive flavonoid tangeretin so that it could be utilized in various food products as functional ingredient. Tangeretin-loaded protein nanoparticles were produced by mixing an organic phase containing zein and tangeretin with an aqueous phase containing ?-lactoglobulin and then converted into powder by freeze-drying. This powder formed a colloidal suspension when dispersed in water that is relatively stable to particle aggregation and sedimentation. The influence of temperature, ionic strength, and pH on the stability of the protein nanoparticles was tested. Extensive particle aggregation occurred at high ionic strength (>100mM) and intermediate pH (4.5-5.5) due to reduced electrostatic repulsion. Extensive aggregation also occurred at temperatures exceeding 60 °C, which was presumably due to increased hydrophobic attraction. Overall, this study shows that protein-based nanoparticles can be used to encapsulate bioactive tangeretin so that it can be readily dispersed in compatible food products.
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The influence of the social support on symptoms of anxiety and depression among patients with silicosis.
ScientificWorldJournal
PUBLISHED: 02-03-2014
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The improvement of social support promotes the mental health and improves the health status. The study aimed to examine the influence of the social support on symptoms of anxiety and depression among patients with silicosis and provide the scientific basis to further alleviate anxiety and depression and to monitor their whole quality of life. We investigated 324 inpatients with silicosis between April 2011 and September 2011. The HADS (the Hospital Anxiety-Depression Scale) was the major methodology used to evaluate anxiety and depression, and the MSPSS (the Multidimensional Scale of Perceived Social Support) to evaluate the social support level. Among patients with silicosis, 99.1% had anxiety symptoms, and 86.1% had depression symptoms. Meanwhile, the social support significantly influenced symptoms of anxiety and depression. The study suggested that patients with silicosis presented more anxiety and depression symptoms, while the social support levels of the patients were relatively low. The influence of social support on symptoms of anxiety and depression among patients with silicosis implied that improving the level of social support and the effective symptomatic treatment might alleviate anxiety and depression symptoms and improve physical and mental status.
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MSI1 regulates survival of hepatoma cell lines by activation of Wnt signaling pathway.
Liver Int.
PUBLISHED: 01-22-2014
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Musashi1(MSI1) belongs to the RNA-binding protein (RBP) family, with functions as translational activator or suppressor of specifically bound mRNA. However, its function in hepatocellular carcinoma (HCC) has been deeply unexplored.
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Galactosylated poly(ethylene glycol) methacrylate-st-3-guanidinopropyl methacrylamide copolymers as siRNA carriers for inhibiting Survivin expression in vitro and in vivo.
J Drug Target
PUBLISHED: 01-09-2014
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In this report, galactosylated poly(ethylene glycol) methacrylate-st-3-guanidinopropyl methacrylamide copolymers (galactosylated PEGMA-st-GPMA, GGP) are developed as siRNA carriers to inhibit Survivin mRNA expression. GGPs are combined with Survivin siRNAs to form siRNA/GGP polyplexes. The polyplexes particles were examined by a dynamic light scattering. It showed that GGP copolymers could condense siRNA to form particles with diameter from 128 to 423?nm and zeta potential value in the range from +2.4 to +14.9?mV at various charge ratios (N/P). The MTT assay data of siRNA/GGP polyplexes on human hepatocellular liver carcinoma cells (HepG2) and human cervix epithelial carcinoma cells (HeLa) indicated that GGP copolymer had better cell viabilities than polyethyleimine (PEI). The transfection of siRNA/GGP polyplexes was detected by real-time quantitative PCR (RT-qPCR) in HepG2 cell line. We found that the siRNA/GGP polyplexes could effectively silence Survivin mRNA expression in the serum-free media (p?
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De novo transcriptome and small RNA analysis of two Chinese willow cultivars reveals stress response genes in Salix matsudana.
PLoS ONE
PUBLISHED: 01-01-2014
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Salix matsudana Koidz. is a deciduous, rapidly growing, and drought resistant tree and is one of the most widely distributed and commonly cultivated willow species in China. Currently little transcriptomic and small RNAomic data are available to reveal the genes involve in the stress resistant in S. matsudana. Here, we report the RNA-seq analysis results of both transcriptome and small RNAome data using Illumina deep sequencing of shoot tips from two willow variants(Salix. matsudana and Salix matsudana Koidz. cultivar 'Tortuosa'). De novo gene assembly was used to generate the consensus transcriptome and small RNAome, which contained 106,403 unique transcripts with an average length of 944 bp and a total length of 100.45 MB, and 166 known miRNAs representing 35 miRNA families. Comparison of transcriptomes and small RNAomes combined with quantitative real-time PCR from the two Salix libraries revealed a total of 292 different expressed genes(DEGs) and 36 different expressed miRNAs (DEMs). Among the DEGs and DEMs, 196 genes and 24 miRNAs were up regulated, 96 genes and 12 miRNA were down regulated in S. matsudana. Functional analysis of DEGs and miRNA targets showed that many genes were involved in stress resistance in S. matsudana. Our global gene expression profiling presents a comprehensive view of the transcriptome and small RNAome which provide valuable information and sequence resources for uncovering the stress response genes in S. matsudana. Moreover the transcriptome and small RNAome data provide a basis for future study of genetic resistance in Salix.
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Enhancement of antitumor immunity using a DNA-based replicon vaccine derived from Semliki Forest virus.
PLoS ONE
PUBLISHED: 01-01-2014
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A DNA-based replicon vaccine derived from Semliki Forest virus, PSVK-shFcG-GM/B7.1 (Fig. 1a) was designed for tumor immunotherapy as previously constructed. The expression of the fusion tumor antigen (survivin and hCG?-CTP37) and adjuvant molecular protein (Granulocyte-Macrophage Colony-Stimulating Factor/ GM-CSF/B7.1) genes was confirmed by Immunofluorescence assay in vitro, and immunohistochemistry assay in vivo. In this paper, the immunological effect of this vaccine was determined using immunological assays as well as animal models. The results showed that this DNA vaccine induced both humoral and cellular immune responses in C57BL/6 mice after immunization, as evaluated by the ratio of CD4+/CD8+ cells and the release of IFN-?. Furthermore, the vaccination of C57BL/6 mice with PSVK-shFcG-GM/B7.1 significantly delayed the in vivo growth of tumors in animal models (survivin+ and hCG?+ murine melanoma, B16) when compared to vaccination with the empty vector or the other control constructs (Fig. 1b). These data indicate that this type of replicative DNA vaccine could be developed as a promising approach for tumor immunotherapy. Meanwhile, these results provide a basis for further study in vaccine pharmacodynamics and pharmacology, and lay a solid foundation for clinical application.
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Enhanced lymphatic transport of bioactive lipids: cell culture study of polymethoxyflavone incorporation into chylomicrons.
Food Funct
PUBLISHED: 10-03-2013
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Polymethoxyflavones (PMFs) are bioactive flavonoids found in citrus fruits that have been shown to have potential health promoting properties. However, their application as nutraceuticals in functional foods and beverages is currently limited due to their low water solubility and high melting point. The oral bioavailability of lipophilic compounds can be enhanced by promoting their intestinal lymphatic transport through co-administration with digestible lipids. We investigated the effects of chylomicron-mediated intestinal lymphatic transport on the bioavailability of 5-hydroxy-6,7,8,3,4-pentamethoxylflavone (5-HPMF), one of representative PMFs in Caco-2 cells. Our results demonstrated that oleic acid and bile acid promoted secretion of chylomicrons in Caco-2 cells, with mean diameter ranged from 70 to 150 nm. The intracellular level of 5-HPMF increased 3-fold by co-incubation with the mixed micelle solution. Moreover, the basolateral level of 5-HPMF increased 3-fold due to enhanced chylomicron-mediated transport. Overall, our results demonstrated for the first time that the bioavailability of polymethoxyflavones can be enhanced by promoting their incorporation into chylomicrons.
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Semi-quantification of surface-enhanced Raman scattering using a handheld Raman spectrometer: a feasibility study.
Analyst
PUBLISHED: 09-24-2013
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The feasibility of utilizing a handheld Raman spectrometer for surface-enhanced Raman scattering detection was evaluated on the pesticide ferbam. A laymans "answer box" was established for semi-quantifying the risk level of ferbam. This study advanced the application of a handheld Raman spectrometer to on-site evaluation of trace amounts of analytes.
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Guanidinylated 3-gluconamidopropyl methacrylamide-s-3-aminopropyl methacrylamide copolymer as siRNA carriers for inhibiting human telomerase reverse transcriptase expression.
Drug Deliv
PUBLISHED: 09-19-2013
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In this report, a series of well-defined glucose- and guanidine-based cationic copolymers as gene carriers were developed to inhibit human telomerase reverse transcriptase (hTERT) gene expression. First of all, guandinylated 3-gluconamidopropyl methacrylamide-s-3-aminopropyl methacrylamide copolymers (guanidinylated GAPMA-s-APMA, abbreviated as GGA) were prepared via aqueous reversible addition--fragmentation chain transfer polymerization (RAFT). Then, three target hTERT siRNA TERT-1, TERT-2 and TERT-3 were designed and combined with GGA copolymers to form siRNA/GGA polyplexes. The polyplexes were examined by dynamic light scattering and agarose gel electrophoresis. The results indicated that GGA copolymers can condense siRNA effectively to form particles with the diameter from 157?nm to 411?nm and zeta potential values in the range from +3.7 to +15.8?mV at various charge ratios (N/P). The MTT assay data of siRNA/GGA polyplexes on human hepatocellular liver carcinoma cells (HepG2) indicated that GGA copolymer had better cell viabilities than polyethylenimine (PEI). Furthermore, the transfection of siRNA/GGA polyplexes was detected by real-time quantitative PCR (RT-qPCR) in HepG2. It was found that siRNA/GGA polyplexes could effectively silence hTERT mRNA expression in serum-free media (p<0.01). In the presence of serum, the hTERT mRNA expression in HepG2 cells have significant difference (p<0.01) between siRNA/GGA3 polyplexes and blank. The results showed that the GAPMA component can reduce the aggregation of protein in serum media. Therefore, the enhancement of transfection may be attributed to the combination of guadino groups and glucose component. And, the guandinylated 3-gluconamidopropyl methacrylamide-s-3-aminopropyl methacrylamide copolymers might be promise in gene delivery.
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Monitoring the chemical production of citrus-derived bioactive 5-demethylnobiletin using surface-enhanced Raman spectroscopy.
J. Agric. Food Chem.
PUBLISHED: 08-20-2013
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To develop an accurate and convenient method for monitoring the production of citrus-derived bioactive 5-demethylnobiletin from the demethylation reaction of nobiletin, we compared surface-enhanced Raman spectroscopy (SERS) methods with a conventional high-performance liquid chromatography (HPLC) method. Our results show that both the substrate- and solution-based SERS methods correlated with the HPLC method very well. The solution method produced lower root-mean-square error of calibration and higher correlation coefficient than the substrate method. The solution method used an "affinity chromatography"-like procedure to separate the reactant nobiletin from the product 5-demthylnobiletin based on their different binding affinities to the silver dendrites. The substrate method was found simpler and faster to collect the SERS "fingerprint" spectra of the samples because no incubation between samples and silver was needed and only a trace amount of samples was required. Our results demonstrated that the SERS methods were superior to the HPLC method in conveniently and rapidly characterizing and quantifying 5-demethylnobiletin production.
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The influence of the functional capacity on subjective well-being and quality of life of patients with silicosis.
Aging Ment Health
PUBLISHED: 04-23-2013
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Silicosis is a fibrosing and irreversible lung disease. In China, 9870 new silicosis cases were reported in 2010. The physical and mental status of patients with silicosis should be paid attention to. The aims of this study were to evaluate the influence of the patients functional capacity on subjective well-being (SWB) and quality of life (QOL), provide the scientific basis for further intervention measures, and improve their health status.
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Antimicrobial ergosteroids and pyrrole derivatives from halotolerant Aspergillus flocculosus PT05-1 cultured in a hypersaline medium.
Extremophiles
PUBLISHED: 04-16-2013
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In order to obtain more structurally novel and bioactive lead compounds for subsequent drug discovery, we have shifted the focus of our study from traditional microbial resources to extremophiles. In this study, a halotolerant fungus Aspergillus flocculosus PT05-1 was isolated from the sediment of Putian saltern of Fujian Province of China in a hypersaline medium. Two new compounds, (22R,23S)-epoxy-3?,11?,14?,16?-tetrahydroxyergosta-5,7-dien-12-one (1) and 6-(1H-pyrrol-2-yl)hexa-1,3,5-trienyl-4-methoxy-2H-pyran-2-one (5) (existed as a pair of epimers with the configuration of 1E,3Z,5E and 1E,3E,5E separately), along with nine known compounds were isolated and identified from the fermentation broth of A. flocculosus PT05-1 grown at a 10 % saline medium. New ergosteroid 1 together with 7-nor-ergosterolide (2) and 3?-hydroxyergosta-8,24(28)-dien-7-one (3) showed cytotoxicity against HL-60 and BEL-7402 cells with IC50 values of 12-18 ?M, and antimicrobial activity against Enterobacter aerogenes, Pseudomonas aeruginosa, and Candida albicans with MIC values of 1.6-15 ?M, respectively. New compound 5 exhibited antibacterial effect on E. aerogenes with MIC value of 3.7 ?M. This study also showed great prospects in developing medicinal resources from extremophiles.
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Impact of 120-W 2-?m continuous wave laser vapoenucleation of the prostate on sexual function.
Lasers Med Sci
PUBLISHED: 04-04-2013
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The objective of this work is to evaluate the impact of 120-W 2-?m continuous wave (cw) laser vapoenucleation of the prostate in patients with benign prostatic hyperplasia (BPH) on sexual function. One hundred twenty-two consecutive patients with BPH were retrospectively collected in this study and were classified into two groups for surgical treatment with 2-?m cw laser vapoenucleation or transurethral resection of the prostate (TURP). International Index of Erectile Function (IIEF) and general assessment questions were completed before and 12 months after treatment to determine the impact on sexual function. A total of 33 patients (52.4 %) in group 1 and 31 (52.5 %) in group 2 reported various degrees of erectile dysfunction before surgery. Interestingly, an increase in IIEF-EF score by 2 points was reported by 16 (25.4 %) and 14 (23.7 %) patients, respectively, and mean EF score did show a marginal but not significant increase postoperatively in both group. Differences about orgasmic intercourse satisfaction, sexual desire domain, and overall satisfaction scores in each group were not significant between preoperative and postoperative, but there was a significant decrease in the orgasmic function domain score at 12 months postoperation in both groups (p?
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Identification of novel bioactive metabolites of 5-demethylnobiletin in mice.
Mol Nutr Food Res
PUBLISHED: 03-20-2013
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Biotransformation of dietary components is important for their in vivo biological activities after oral ingestion. Herein, we investigated biotransformation of 5-demethylnobiletin (a polymethoxyflavone found in citrus fruits) in mice, and its implication in the inhibition of human colon cancer cells.
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5-Demethyltangeretin inhibits human nonsmall cell lung cancer cell growth by inducing G2/M cell cycle arrest and apoptosis.
Mol Nutr Food Res
PUBLISHED: 02-21-2013
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Tangeretin (TAN) and 5-demethyltangeretin (5DT) are two closely related polymethoxyflavones found in citrus fruits. We investigated growth inhibitory effects on three human nonsmall cell lung cancer (NSCLC) cells.
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The effects of Gd3+ substitution on the crystal structure, site symmetry, and photoluminescence of Y/Eu layered rare-earth hydroxide (LRH) nanoplates.
Dalton Trans
PUBLISHED: 12-14-2011
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Well crystallized nanoplates of the (Y(0.95-x)Gd(x)Eu(0.05))(2)(OH)(5)NO(3)·nH(2)O ternary layered rare-earth hydroxides (LRHs), synthesized hydrothermally, have been investigated with emphasis on the effects of Gd(3+) substitution for Y(3+) on the structural features and optical properties. Characterizations of the materials were achieved by the combined techniques of XRD, FT-IR, TEM, DTA/TG, and optical spectroscopies. The results showed that Gd(3+) substitution leads to linearly expanded ab plane, shortened interlayer distance (c/2), and reduced hydration (smaller n value) of the crystal structure. As a consequence, the Ln(3+) partially shifts from the C(4v) to C(1) site symmetries and thus leads to systematically altered photoluminescence behaviors. Under the (7)F(0)?(5)L(6) transition excitation of Eu(3+) at 394 nm, both the (5)D(0)?(7)F(2) to (5)D(0)?(7)F(4) and the 595 nm (5)D(0)?(7)F(1) to 590 nm (5)D(0)?(7)F(1) intensity ratios linearly increase towards a higher Gd(3+) content. The incorporated Gd(3+) cations selectively sensitize emission from the C(1)-site Eu(3+) and produce a new charge transfer (CT) excitation band at ?254 nm. With this, the desired 615-nm red emission is obtainable either under intra-4f(6) transition excitation of Eu(3+) or by exciting the CT band. The materials have similar fluorescence lifetimes of 0.85 ± 0.05 ms for the 615-nm emission, irrespective of the Gd(3+) content and excitation wavelength.
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Assessment of respiration-related quality of life of Chinese patients with silicosis and its influencing factors using the St. Georges Respiratory Questionnaire (SGRQ).
J Clin Nurs
PUBLISHED: 11-24-2011
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The aims are to assess respiration-related quality of life using the St. George Respiratory Questionnaire and determine its influencing factors among patients with silicosis in China.
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Nanoemulsion-based delivery systems for poorly water-soluble bioactive compounds: Influence of formulation parameters on Polymethoxyflavone crystallization.
Food Hydrocoll
PUBLISHED: 09-17-2011
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Polymethoxyflavones (PMFs) extracted from citrus peel exhibit potent anti-cancer activity, but are highly hydrophobic molecules with poor solubility in both water and oil at ambient and body temperature, which limits their bioavailability. The possibility of encapsulating PMFs within nanoemulsion-based delivery systems to facilitate their application in nutraceutical and pharmaceutical products was investigated. The influence of oil type (corn oil, MCT, orange oil), emulsifier type (?-lactoglobulin, lyso-lecithin, Tween, and DTAB), and neutral cosolvents (glycerol and ethanol) on the formation and stability of PMF-loaded nanoemulsions was examined. Nanoemulsions (r < 100 nm) could be formed using high pressure homogenization for all emulsifier types, except DTAB. Lipid droplet charge could be altered from highly cationic (DTAB), to near neutral (Tween), to highly anionic (?-lactoglobulin, lyso-lecithin) by varying emulsifier type. PMF crystals formed in all nanoemulsions after preparation, which had a tendency to sediment during storage. The size, morphology, and aggregation of PMF crystals depended on preparation method, emulsifier type, oil type, and cosolvent addition. These results have important implications for the development of delivery systems for bioactive components that have poor oil and water solubility at application temperatures.
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Three new compounds from Aspergillus terreus PT06-2 grown in a high salt medium.
Mar Drugs
PUBLISHED: 06-01-2011
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To investigate the structurally novel and bioactive natural compounds from marine-derived microorganisms under high salinity, the fungus Aspergillus terreus PT06-2 was isolated from the sediment of the Putian Sea Saltern, Fujian, China. Three new compounds, terremides A (1) and B (2) and terrelactone A (3), along with twelve known compounds (4-15) were isolated and identified from the fermentation broth of A. terreus PT06-2 at 10% salinity. Among these metabolites, compounds 4 and 15 only produced in the 10% salinity culture, were identified as methyl 3,4,5-trimethoxy-2-(2-(nicotinamido) benzamido) benzoate, and (+)-terrein, respectively. The new compounds 1 and 2 exhibited antibacterial activity against Pseudomonas aeruginosa and Enterobacter aerogenes with MIC values of 63.9 and 33.5 ?M, respectively. Compounds 5 showed moderate anti-H1N1 activity and lower cytotoxicity with IC?? and CC?? values of and 143.1 and 976.4 ?M, respectively.
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The inhibitory effects of 5-hydroxy-3,6,7,8,3,4-hexamethoxyflavone on human colon cancer cells.
Mol Nutr Food Res
PUBLISHED: 01-30-2011
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Previously, we reported that 5-hydroxy-3,6,7,8,3,4-hexamethoxyflavone (5HHMF), a polymethoxyflavone found in citrus peels, potently inhibited the growth of multiple human colon cancer cells. Herein, we further investigated the anti-cancer mechanisms of 5HHMF in human colon cancer cells.
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Cyclic tripeptides from the halotolerant fungus Aspergillus sclerotiorum PT06-1.
J. Nat. Prod.
PUBLISHED: 05-28-2010
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Eleven new aspochracin-type cyclic tripeptides, sclerotiotides A-K (1-11), together with three known compounds, JBIR-15 (12), aspochracin (13), and penicillic acid, were isolated from the ethyl acetate extract of the fermentation broth of the halotolerant Aspergillus sclerotiorum PT06-1 in a hypersaline nutrient-rich medium. Their structures were elucidated by spectroscopic analysis and chemical methods. Chemical transformations of 12 and 13 proved that sclerotiotides D-K (4-11) were artifacts probably formed during the fermentation or subsequent isolation steps. All 13 cyclic tripeptides have been evaluated for their antimicrobial and cytotoxic effects. Only sclerotiotides A (1), B (2), F (6), and I (9) and JBIR-15 (12) showed selective antifungal activity against Candida albicans with MIC values of 7.5, 3.8, 30, 6.7, and 30 microM, respectively.
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Long-term remission after successful pregnancy in autologous peripheral blood stem cell transplanted system lupus erythematosus patients.
Rheumatol. Int.
PUBLISHED: 02-25-2010
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Pregnancies in systemic lupus erythematosus (SLE) patients are high risk to both mother and fetus because of increased rates of complications. During the past years, we have treated many cases of SLE patients using autologous peripheral blood stem cell transplantation with good outcome after pregnancy. The rate of maternal hypertension and lupus nephritis was greatly reduced in autologous peripheral blood stem cell transplanted group (n = 11) when compared to non-transplant group (n = 39) (P < 0.05). In addition, the outcome of lupus flare activity of the mother after delivery is significantly better in transplanted group than that in non-transplanted group (P < 0.05). Here, we describe two typical cases of long duration (>6 years) of remission after successful pregnancy in refractory SLE patients post-autologous peripheral blood stem cell transplantation. Our report demonstrated that peripheral blood stem cell transplantation is safe and effective, thereby could be recommended as prior strategy in refractory SLE patients, especially for those women of child-bearing age who plan for pregnancy.
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Novel cyclic hexapeptides from marine-derived fungus, Aspergillus sclerotiorum PT06-1.
Org. Lett.
PUBLISHED: 10-16-2009
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Two novel cyclic hexapeptides containing both anthranilic acid and dehydroamino acid units, sclerotides A (1) and B (2), were isolated from the marine-derived halotolerant Aspergillus sclerotiorum PT06-1 in a nutrient-limited hypersaline medium. Both 1 and 2 are photointerconvertible and could be interconverted via a radical reaction initiated by direct photoisomerization. Both compounds showed moderate antifungal activity. Compound 2 also showed weak cytotoxicity and antibacterial activity.
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Genome-wide DNA methylation analyses in the brain reveal four differentially methylated regions between humans and non-human primates.
BMC Evol. Biol.
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The highly improved cognitive function is the most significant change in human evolutionary history. Recently, several large-scale studies reported the evolutionary roles of DNA methylation; however, the role of DNA methylation on brain evolution is largely unknown.
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Influence of particle size on performance of a pilot-scale fixed-bed gasification system.
Bioresour. Technol.
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The effect of particle size on the gasification performance of a pilot-scale (25 kg/h) downdraft fixed bed gasification system was investigated using prunings from peach trees at five different size fractions (below 1, 1-2, 2-4, 4-6 and 6-8 cm). The gas and hydrocarbon compositions were analyzed by gas chromatography (GC) and gas chromatography/mass spectrometry (GC-MS), respectively. With increasing particle size, gas yield increased while tar and dust content decreased. The lower heating value of the gas decreased slightly with particle size. At a smaller particle size, more hydrocarbons were detected in the producer gas. Hydrogen and carbon dioxide contents increased with the decrease in particle size, reaching 16.09% and 14.36% at particle size below 1cm, respectively. Prunings with a particle size of 1-2 cm were favorable for gasification in the downdraft gasifier used in this study.
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[Construction of an anti-tumor immunogene vaccine pVAX-IL-12-GB and its expression in vivo and in vitro].
Nan Fang Yi Ke Da Xue Xue Bao
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To construct a novel immunogene therapeutic plasmid that expresses human interleukin-12 (IL-12), granulocyte-macrophage colony stimulating factor (GM-CSF) and B7.1 and observe its expression in vivo and in vitro.
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[Prokaryotic expression, purification and antigenicity identification of mouse prostate stem cell antigen].
Nan Fang Yi Ke Da Xue Xue Bao
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To amplify mouse prostate stem cell antigen (mPSCA) gene and construct a recombinant plasmid to obtain mPSCA protein and identify its antigenicity.
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Transcriptome landscape of the human placenta.
BMC Genomics
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The placenta is a key component in understanding the physiological processes involved in pregnancy. Characterizing genes critical for placental function can serve as a basis for identifying mechanisms underlying both normal and pathologic pregnancies. Detailing the placental tissue transcriptome could provide a valuable resource for genomic studies related to placental disease.
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Synergistic anti-inflammatory effects of nobiletin and sulforaphane in lipopolysaccharide-stimulated RAW 264.7 cells.
J. Agric. Food Chem.
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Inflammation plays important roles in the initiation and progress of many diseases including cancers in multiple organ sites. Herein, we investigated the anti-inflammatory effects of two dietary compounds, nobiletin (NBN) and sulforaphane (SFN), in combination. Noncytotoxic concentrations of NBN, SFN, and their combinations were studied in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophage cells. The results showed that combined NBN and SFN treatments produced much stronger inhibitory effects on the production of nitric oxide (NO) than NBN or SFN alone at higher concentrations. These enhanced inhibitory effects were synergistic based on the isobologram analysis. Western blot analysis showed that combined NBN and SFN treatments synergistically decreased iNOS and COX-2 protein expression levels and induced heme oxygenase-1 (HO-1) protein expression. Real-time polymerase chain reaction analysis indicated that low doses of NBN and SFN in combination significantly suppressed LPS-induced upregulation of IL-1 mRNA levels and synergistically increased HO-1 mRNA levels. Overall, our results demonstrated that NBN and SFN in combination produced synergistic effects in inhibiting LPS-induced inflammation in RAW 264.7 cells.
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What is Visualize?

JoVE Visualize is a tool created to match the last 5 years of PubMed publications to methods in JoVE's video library.

How does it work?

We use abstracts found on PubMed and match them to JoVE videos to create a list of 10 to 30 related methods videos.

Video X seems to be unrelated to Abstract Y...

In developing our video relationships, we compare around 5 million PubMed articles to our library of over 4,500 methods videos. In some cases the language used in the PubMed abstracts makes matching that content to a JoVE video difficult. In other cases, there happens not to be any content in our video library that is relevant to the topic of a given abstract. In these cases, our algorithms are trying their best to display videos with relevant content, which can sometimes result in matched videos with only a slight relation.