JoVE Visualize What is visualize?
Stop Reading. Start Watching.
Advanced Search
Stop Reading. Start Watching.
Regular Search
Find video protocols related to scientific articles indexed in Pubmed.
New Risk Factors for Adult Onset Incident Asthma: A Nested Case Control Study of Host Antioxidant Defense.
Am. J. Respir. Crit. Care Med.
PUBLISHED: 11-20-2014
Show Abstract
Hide Abstract
Rationale: Host antioxidant defense, consisting of enzymatic antioxidant activity and non-enzymatic antioxidant micronutrients, is implicated in asthma pathogenesis. Studies of antioxidant defense and adult incident asthma have either used measures of antioxidants estimated from questionnaires or not considered enzymatic aspects of host defense. Objective: We conducted the first study designed and powered to investigate the association of antioxidant defenses on adult incident asthma. Methods: In a nested case-control study, we followed Shanghai women (ages 40-70) without prevalent asthma at baseline, over eight years. Incident asthmatics were ascertained prospectively by gold standard testing of symptomatic women and matched to two asymptomatic controls. Measurements: Baseline urinary F2-isoprostanes, plasma concentrations of antioxidant micronutrients (tocopherols, xanthins, carotenes, and lycopene) and antioxidant enzyme activity (platelet-activating factor acetylhydrolase [PAF-AH] and superoxide dismutase) were measured from samples collected prior to disease onset. Main Results: Among 65,372 women, 150 (0.24%) developed asthma. F2-isoprostane levels prior to asthma onset were not different between cases and controls. Doubling of ?-tocopherol concentrations and PAF-AH activity was associated with 50% and 37% decreased risk of incident asthma (adjusted OR; 95% confidence interval [OR; 95%CI], ?-tocopherol OR= 0.52; 95%CI: 0.32-0.84; PAF-AH OR=0.63; 95%CI:0.42-0.93). Conclusions: In this prospective study ?-tocopherol, within normal reference ranges, and PAF-AH enzymatic activity, were associated with decreased asthma development. These modifiable risk factors may be an effective strategy to test for primary asthma prevention.
Related JoVE Video
[Therapeutic efficacy and safety of chemotherapy subsequent EGFR-TKI in advanced non-small cell lung cancer patients with EGFR-TKI acquired resistance].
Zhonghua Yi Xue Za Zhi
PUBLISHED: 11-18-2014
Show Abstract
Hide Abstract
To explore the therapeutic efficacy and safety of chemotherapy subsequent epidermal growth factor receptor-tyrosine kinase inhibitor (EGFR-TKI) in advanced non-small cell lung cancer (NSCLC) patients with EGFR-TKI acquired resistance.
Related JoVE Video
Application of Acellular Allograft Dermal Matrix in Breast Implants for Secondary Breast Deformity post Polyacrylamide Hydrogel Removal.
Zhongguo Yi Xue Ke Xue Yuan Xue Bao
PUBLISHED: 11-01-2014
Show Abstract
Hide Abstract
Objective To evaluate the safety and efficiency of acellular dermal matrix combined with implant in correcting the secondary deformity post polyacrylamide hydrogel removal. Methods A retrospective study enrolling 23 female patients(42 breasts)who received breast deformity correction from June 2008 to June 2012 with acellular dermal matrix and implant were performed. Results One patient suffered bilateral seroma and 1 patient suffered infection in one side. None of the implants were removed. Patients were followed up for(15.0±11.7)months(range:3-36 months),and the results were satisfactory. Conclusion Acelluar dermal matrix and implant can be safely used simultaneously in correcting the secondary breast deformity post polyacrylamide hydrogel removal.
Related JoVE Video
Synergistic Coupling of Fluorescent "Turn-Off" with Spectral Overlap Modulated FRET for Ratiometric Ag(+) Sensor.
Inorg Chem
PUBLISHED: 10-27-2014
Show Abstract
Hide Abstract
A useful strategy for ratiometric fluorescent detecting of Ag(+) is demonstrated. Upon selective binding of Ag(+) to a BODIPY-porphyrin dyad (1), the synergistic coupling of two functions, namely the suppressing of FRET from BODIPY donor to porphyrin acceptor and the fluorescence quenching of porphyrin acceptor, leads to exceptionally large changes in the intensity ratio of two distinct emissions (F513/F654) which allow for the ratiometric detecting of Ag(+) with excellent sensitivity in solution and living cells.
Related JoVE Video
Identification of Serine 348 on the Apelin Receptor as a Novel Regulatory Phosphorylation Site in Apelin-13-induced G Protein-independent Biased Signaling.
J. Biol. Chem.
PUBLISHED: 09-30-2014
Show Abstract
Hide Abstract
Phosphorylation plays vital roles in the regulation of G protein-coupled receptor (GPCR) functions. The apelin and apelin receptor (APJ) system is involved in the regulation of cardiovascular function and central control of body homeostasis. Here, using tandem mass spectrometry, we first identified phosphorylated serine residues in the C terminus of APJ. To determine the role of phosphorylation sites in APJ-mediated G protein-dependent and -independent signaling and function, we induced a mutation in the C-terminal serine residues and examined their effects on the interaction between APJ with G protein or GRK/?-arrestin and their downstream signaling. Mutation of serine 348 led to an elimination of both GRK and ?-arrestin recruitment to APJ induced by apelin-13. Moreover, APJ internalization and G protein-independent ERK signaling were also abolished by point mutation at serine 348. In contrast, this mutant at serine residues had no demonstrable impact on apelin-13-induced G protein activation and its intracellular signaling. These findings suggest that mutation of serine 348 resulted in inactive GRK/?-arrestin. However, there was no change in the active G protein thus, APJ conformation was biased. These results provide important information on the molecular interplay and impact of the APJ function, which may be extrapolated to design novel drugs for cardiac hypertrophy based on this biased signal pathway.
Related JoVE Video
Dynamics of apelin receptor/G protein coupling in living cells.
Exp. Cell Res.
PUBLISHED: 09-01-2014
Show Abstract
Hide Abstract
During our research on apelin receptor (APJ) signalling in living cells with BRET and FRET, we demonstrated that apelin-13 stimulation can lead to the activation of G?i2 or G?i3 through undergoing a molecular rearrangement rather than dissociation in HEK293 cells expressing APJ. Furthermore, G?o and G?q also showed involvement in APJ activation through a classical dissociation model. However, both FRET signal and BRET ratio between fluorescent G?i1 subunit and G?? subunits demonstrated little change after apelin-13 stimulation. These results demonstrated that stimulation of APJ with apelin-13 causes activation of G?i2, G?i3, G?o, G?q; among which G?i2, G?i3 were activated through a novel rearrangement process. These results provide helpful data for understanding APJ mediated G-protein signalling.
Related JoVE Video
Heterodimerization of apelin receptor and neurotensin receptor 1 induces phosphorylation of ERK1/2 and cell proliferation via G?q-mediated mechanism.
J. Cell. Mol. Med.
PUBLISHED: 08-28-2014
Show Abstract
Hide Abstract
Dimerization of G protein-coupled receptors (GPCRs) is crucial for receptor function including agonist affinity, efficacy, trafficking and specificity of signal transduction, including G protein coupling. Emerging data suggest that the cardiovascular system is the main target of apelin, which exerts an overall neuroprotective role, and is a positive regulator of angiotensin-converting enzyme 2 (ACE2) in heart failure. Moreover, ACE2 cleaves off C-terminal residues of vasoactive peptides including apelin-13, and neurotensin that activate the apelin receptor (APJ) and neurotensin receptor 1 (NTSR1) respectively, that belong to the A class of GPCRs. Therefore, based on the similar mode of modification by ACE2 at peptide level, the homology at amino acid level and the capability of forming dimers with other GPCRs, we have been suggested that APJ and NTSR1 can form a functional heterodimer. Using co-immunoprecipitation, BRET and FRET, we provided conclusive evidence of heterodimerization between APJ and NTSR1 in a constitutive and induced form. Upon agonist stimulation, hetrodimerization enhanced ERK1/2 activation and increased proliferation via activation of Gq ?-subunits. These novel data provide evidence for a physiological role of APJ/NTSR1 heterodimers in terms of ERK1/2 activation and increased intracellular calcium and induced cell proliferation and provide potential new pharmaceutical targets for cardiovascular disease.
Related JoVE Video
Transjugular Intrahepatic Portosystemic Shunt for Acute Variceal Bleeding: A Meta-analysis.
J. Clin. Gastroenterol.
PUBLISHED: 08-14-2014
Show Abstract
Hide Abstract
Acute variceal bleeding is the most common lethal complication of liver cirrhosis. A meta-analysis was conducted to compare the outcomes of transjugular intrahepatic portosystemic shunt (TIPS) to those of medical/endoscopic therapy for acute variceal bleeding in cirrhotic patients.
Related JoVE Video
Roxithromycin treatment inhibits TGF-?1-induced activation of ERK and AKT and down-regulation of caveolin-1 in rat airway smooth muscle cells.
Respir. Res.
PUBLISHED: 08-11-2014
Show Abstract
Hide Abstract
Roxithromycin (RXM) has been widely used in asthma treatment; however, the mechanism has not been fully understood. The aim of our study was to investigate the underlying mechanism of RXM treatment in mediating the effect of transforming growth factor (TGF)-?1 on airway smooth muscle cells (ASMCs) proliferation and caveolinn-1 expression.
Related JoVE Video
Heterozygote advantage of methylenetetrahydrofolate reductase polymorphisms on clinical outcomes in advanced non-small cell lung cancer (NSCLC) patients treated with platinum-based chemotherapy.
Tumour Biol.
PUBLISHED: 08-08-2014
Show Abstract
Hide Abstract
Methylenetetrahydrofolate reductase (MTHFR) enzyme is essential for transmethylation reactions including DNA methylation and DNA synthesis and thereby may contribute to cancer prognosis. In our study, a total of 1,004 advanced non-small cell lung cancer (NSCLC) patients receiving first-line, platinum-based chemotherapy regimens were used for genotyping 10 tag single nucleotide polymorphisms (SNPs) of MTHFR. Association was assessed between the SNPs and treatment outcomes. We found that polymorphism of rs1537514 showed the most significant effect: heterozygote associated with better clinical benefit (P?=?0.002) and decreased risk of grade 3 or 4 gastrointestinal toxicity (P?=?0.027), while the mutant homozygote associated with increased risk of severe gastrointestinal toxicity (P?=?0.031) and thrombocytopenia (P?=?0.009). The heterozygotes of exon polymorphisms (rs1801131, rs1801133) also yielded better clinical benefit (P?=?0.030 for rs1801131) and decreased risk of severe gastrointestinal toxicity (P?=?0.004 for rs1801131) or thrombocytopenia (P?=?0.016 for 1801133). However, overall survival (OS) and progression-free survival (PFS) did not differ for the MTHFR polymorphisms, except for heterozygote of rs1537514 showing significant effects with better PFS (P?=?0.022). Clinical factors as age, gender, and smoking status had significant effects for the OS (P?=?0.003, 0.002, and 0.012, respectively) while performance status and chemotherapy regimens for PFS (P?=?0.001 and 3.9?×?10(-6), respectively). The results indicate that a heterozygous advantage may exist in certain MTHFR variants, and the polymorphisms (especially rs1537514) may play a predictive role of treatment efficacy and adverse effects in NSCLC patients treated with platinum-based chemotherapy.
Related JoVE Video
Construction of doxycycline-mediated BMP-2 transgene combining with APA microcapsules for bone repair.
Artif Cells Nanomed Biotechnol
PUBLISHED: 08-06-2014
Show Abstract
Hide Abstract
Objectives: The repairing of large segmental bone defects is difficult for clinical orthopedists at present. Gene therapy is regarded as a promising method for bone defects repair. The present study aimed to construct an effective and controllable Tet-On expression system for transferring hBMP-2 gene into bone marrow mesenchymal progenitor cells (BMSCs). Meanwhile, with combination of alginate-poly-L-lysine-alginate (APA) microencapsulation technology, we attempted to reduce the influence of immunologic rejection and examine the effect of the Tet-On expression system on osteogenesis of BMSCs. Methods: The adenovirus encoding hBMP-2 (ADV-hBMP2) was constructed using the means of molecular cloning. The ADV-hBMP2 and Adeno-X Tet-On virus was respectively transfected to the HEK293 for amplification and afterward BMSCs were co-infected with the virus of ADV-hBMP2 and the Adeno-X Tet-On. The expression of hBMP-2 was measured with induction by doxycycline (DOX) at different concentration by means of RT-PCR and ELISA. Combining Tet-On expression system and APA microcapsules with the use of the pulsed high-voltage electrostatic microcapsule instrument, we examined the expression level of hBMP-2 in APA microcapsules by ELISA as well as the osteogenesis by alizarin red S staining. Key findings: An effective Tet-On expression system for transferring hBMP-2 gene into BMSCs was constructed successfully. Also, the expression of hBMP-2 could be regulated by concentration of DOX. The data exhibited that BMSCs in APA microcapsules maintained the capability of proliferation and differentiation. The combination of Tet-On expression system and APA microcapsules could promote the osteogenesis of BMSCs. Conclusions: According to the results, microencapsulated Tet-On expression system showed the effective characteristics of secreting hBMP-2 and enhancing osteogenesis, which would provide a promising way for bone repair.
Related JoVE Video
Antioxidant and anti-inflammatory active dihydrobenzofuran neolignans from the seeds of Prunus tomentosa.
J. Agric. Food Chem.
PUBLISHED: 07-24-2014
Show Abstract
Hide Abstract
Prunus tomentosa seeds were researched for antioxidant and anti-inflammatory constituents. By activity-guided fractionation of P. tomentosa seed extract, six new dihydrobenzofuran neolignans, prunustosanans AI-IV (1-4) and prunustosanansides AI and AII (5 and 6), together with 10 known compounds (7-16) were isolated from bioactive fraction. The structures were determined by spectroscopic analyses, especially NMR, HRESIMS, and CD spectra. The antioxidant activity was greatest for 5, 10, and 12 against DPPH radical and for 8, 9, and 13 against ABTS radical. Moreover, compounds 7 and 11 exhibited much stronger inhibitory activity on nitric oxide (NO) production in murine microglia BV-2 compared with positive control minocycline (IC50 = 19.7 ± 1.5 ?M). The results show that P. tomentosa seeds can be regarded as a potential source of antioxidants and inflammation inhibitors.
Related JoVE Video
An update to the taxonomy of the genus Gastroserica Brenske (Coleoptera, Scarabaeidae, Sericini).
Zookeys
PUBLISHED: 07-17-2014
Show Abstract
Hide Abstract
Based on the examination of newly collected material and additional specimens housed in Chinese collections, our knowledge of Gastroserica Brenske, 1897, is expanded. Here, seven new species are described, including habitus photographs and illustrations of the male genitalia: Gastroserica haoyui sp. n. (China: Zhejiang Prov.), G. fengduana sp. n. (China: Sichuan Prov.), G. wenzhui sp. n. (China: Guangxi Prov.), G. damingshanica sp. n. (from China: Guangxi Prov.), G. jinxiuensis sp. n. (China: Guangxi Prov.), G. liboensis sp. n. (China: Yunnan Prov.) and G. carolusi sp. n. (Laos). Additionally, we provide a distribution map of the new taxa and new distribution records of the known taxa.
Related JoVE Video
Neuroprotective effects of apelin-13 on experimental ischemic stroke through suppression of inflammation.
Peptides
PUBLISHED: 07-13-2014
Show Abstract
Hide Abstract
Acute inflammation plays an important role in the pathogenic progression of post-ischemic neuronal damage. Apelin-13 has been investigated as a neuropeptide for various neurological disorders. The present study was performed to evaluate the effects of apelin-13 on the inflammation of cerebral ischemia/reperfusion (I/R) injury. Transient focal I/R model in male Wistar rats were induced by 2h middle cerebral artery occlusion (MCAO) followed by 24h reperfusion. Rats then received treatment with apelin-13 or vehicle after ischemia at the onset of reperfusion. The neurological deficit was evaluated and the infarct volume was measured by TTC staining. The activity of myeloperoxidase (MPO) was measured. The expression of pro-inflammatory cytokines including tumor necrosis factor-? (TNF-?), interleukin-1? (IL-1?), and intercellular adhesion molecule-1 (ICAM-1) were measured using real-time PCR. And the expression of apelin receptor (APJ), ionized calcium-binding adapter molecule-1 (Iba1), glial fibrillary acidic protein (GFAP) and high mobility group box 1 (HMGB1) were measured by immunohistochemistry and western blot. Our results demonstrated that treatment with apelin-13 in I/R rats markedly reduced neurological deficits and the infarct volume. The increase of MPO activity induced by I/R was inhibited by apelin-13 treatment. The real-time PCR showed that apelin-13 decreased the expression of inflammatory cytokines such as IL-1?, TNF-? and ICAM-1 in I/R rats. The expression of APJ in I/R rats was increased. And the expression of Iba1, GFAP and HMGB1 in I/R rats was decreased by apelin-13 treatment indicating the inhibition of microglia, astrocytes and other inflammatory cells. In conclusion, apelin-13 is neuroprotective for neurons against I/R through inhibiting the neuroinflammation.
Related JoVE Video
Miniscalpel-Needle versus Steroid Injection for Plantar Fasciitis: A Randomized Controlled Trial with a 12-Month Follow-Up.
Evid Based Complement Alternat Med
PUBLISHED: 07-08-2014
Show Abstract
Hide Abstract
Plantar fasciitis is the most common cause of heel pain in adults. A novel alternative medical instrument, the miniscalpel-needle (MSN), which is based on an acupuncture needle, has been recently developed in China. The objective of this study was to evaluate the effectiveness of the MSN release treatment versus that of traditional steroid injection for plantar fasciitis. Patients with plantar fasciitis were randomly assigned to 2 groups and followed up for 12 months, with 29 receiving MSN treatment and 25 receiving steroid injection treatment. The results showed that visual analog scale scores for morning pain, active pain, and overall heel pain all were decreased significantly in the MSN group from 1 to 12 months after treatment. In contrast, treatment with steroid injection showed a significant effect only at the 1-month follow-up but not at 6 or 12 months after treatment. Moreover, the MSN group achieved more rapid and sustained improvements than the steroid group throughout the duration of this study. No severe side effects were observed with MSN treatment. Our data suggest that the MSN release treatment is safe and has a significant benefit for plantar fasciitis compared to steroid injection.
Related JoVE Video
[Apoptosis-inducing effect of tetrandrine and imatinib on K562/G01 cells and its related mechanism].
Zhongguo Shi Yan Xue Ye Xue Za Zhi
PUBLISHED: 07-04-2014
Show Abstract
Hide Abstract
This study was purposed to explore the apoptosis-inducing effect of tetrandrine (Tet) and imatinib (IM) alone or both combined on K562/G01 cells and their mechanism. MTT assay was used to detect the inhibitory effect of drugs on cell growth, flow cytometry was used to detect the cell cycle and apoptosis rate. The expression of caspase-3/BCL-2 mRNA was determined by real time-PCR, and the expression of caspase-3/BCL-2 protein was assayed by Western blot. The results showed that after being treated by 1.0 µmol/L IM or 1.5 µmol/L Tet alone and combination of these two drugs for 48 h, the inhibitory rate was (22.74 ± 0.05)%, (20.34 ± 0.57)% and (44.28 ± 0.60)%, respectively, suggesting that inhibitory effect of two drug combination was more obvious. The arrest of cell cycle at G1/S phase could be observed after Tet treatment. Early apoptosis rate was (7.81 ± 0.16) %, (14.10 ± 0.28) % respectively after being treated by combination of 1.5 µmol/L and 3.0 µmol/L Tet with 1.0 µmol/L IM. After being treated with Tet alone, FQ-PCR and Western blot showed that the expressions of caspase-3 mRNA and caspase-3 protein were up-regulated, the expressions of BCL-2 mRNA and protein were down-regulated, the effect of both drug combination was more significant. It is concluded that IM or Tet alone can induce apoptosis of K562/G01. Combination of IM with Tet shows obvious synergistic effect, mechanism of which may associate with up-regulation of caspase-3 mRNA and protein expressions, and down-regulation of BCL-2 mRNA and protein expressions.
Related JoVE Video
Transarterial chemoembolization combined with sorafenib for unresectable hepatocellular carcinoma: a systematic review and meta-analysis.
Mol. Biol. Rep.
PUBLISHED: 06-19-2014
Show Abstract
Hide Abstract
Sorafenib in combination with Transarterial chemoembolization (TACE) is increasingly used in patients with unresectable hepatocellular carcinoma (HCC), but the current evidence is still controversial. The aim of this systematic review was to evaluate the effectiveness and safety of TACE plus sorafenib versus TACE alone for unresectable HCC. We searched PubMed, EMBASE and the Cochrane Library for clinical trials comparing TACE plus sorafenib with TACE alone for unresectable HCC. The study outcomes included overall survival (OS), time to progression (TTP), objective response and adverse events (AEs). Six studies including 1,181 patients were included. Meta-analysis of all studies suggested that the combination therapy group had significant longer OS than TACE group [hazard ratio (HR) = 0.64, 95 % confidence interval (CI) = 0.43-0.97], but the pooled HR of randomized controlled trials (RCTs) failed to achieve statistical significance. For TTP, meta-analysis in both RCTs subgroup and retrospective studies subgroup suggested that combination therapy was superior to TACE group. The combination therapy was also associated with better response to treatment (risk ratio = 1.45, 95 % CI = 1.04-2.02) when both RCTs and retrospective studies were pooled. However, the sorafenib associated AEs were more frequent in the combination therapy group. In conclusion, the combination of TACE and sorafenib is likely to improve OS, TTP and response to treatment when compared with TACE monotherapy. The combination group is also associated with more sorafenib-related AEs.
Related JoVE Video
Effects of parathyroid hormone on calcium ions in rat bone marrow mesenchymal stem cells.
Biomed Res Int
PUBLISHED: 06-18-2014
Show Abstract
Hide Abstract
The present study was conducted in order to explore the mechanisms whereby parathyroid hormone (PTH) maintains in vitro proliferation of bone marrow mesenchymal stem cells (BMSCs). Bone marrow was isolated from Sprague Dawley (SD) rat femurs, cultured in vitro, and passaged using a cell adherent culture method. The BMSC proliferation was evaluated by the methyl thiazolyl tetrazolium (MTT) assay and the fluorescence intensity of calcium ions in BMSCs was analyzed by laser scanning confocal microscopy (LSCM). Our results show that BMSC proliferation in the experimental group treated with PTH was more significant than controls. The calcium ion fluorescence intensity in BMSCs was significantly higher for the experimental group as compared to the control group. For each group, there was significant difference in the fluorescence intensity of calcium ions in BMSCs between 7?d and 14?d. In conclusion, parathyroid hormone increased the fluorescence intensity of calcium ions in BMSCs, which might represent a key mechanism whereby BMSC proliferation is maintained.
Related JoVE Video
Pharmacologic prophylaxis of portal venous system thrombosis after splenectomy: a meta-analysis.
Gastroenterol Res Pract
PUBLISHED: 06-13-2014
Show Abstract
Hide Abstract
Portal venous system thrombosis (PVST) is a life-threatening complication of splenectomy. A meta-analysis was conducted to explore the role of pharmacologic prophylaxis of PVST after splenectomy. Overall, 359 papers were initially identified via the PubMed, EMBASE, and Cochrane Library databases. Eight of them were eligible. The incidence of PVST after splenectomy was significantly lower in patients who received the preventive measures than in those who did not (odds ratio [OR]: 0.33, 95% confidence interval [CI]: 0.22-0.47, P < 0.00001). Subgroup analyses demonstrated that the significant difference remained in studies including patients with portal hypertension (n = 6), but not in those including patients with hematological diseases (n = 2); the significant difference remained in studies using any type of prophylactic drugs (anticoagulants [n = 6], thrombolytics [n = 1], and prostaglandin E1 [n = 1]); the significant difference remained in nonrandomized studies (n = 5), but not in randomized studies (n = 3). The risk of bleeding was similar between the two groups (OR: 0.65, 95% CI: 0.10-4.04, P = 0.64). In conclusion, pharmacologic prophylaxis might decrease the incidence of PVST after splenectomy in patients with portal hypertension and did not increase the risk of bleeding. However, the effect of pharmacologic prophylaxis of PVST in patients with hematological diseases remained questioned.
Related JoVE Video
[Triptolide affect the methylation status of HL-60cells].
Zhonghua Xue Ye Xue Za Zhi
PUBLISHED: 05-27-2014
Show Abstract
Hide Abstract
To study the effect of triptolide (TP) on the methylation status of human promyelocytic leukemia cells (HL-60) and explore a preliminary demethylation mechanism.
Related JoVE Video
Mechanisms of behavior modification in clinical behavioral medicine in China.
Int J Behav Med
PUBLISHED: 05-21-2014
Show Abstract
Hide Abstract
Behavior modification, as the core of clinical behavioral medicine, is often used in clinical settings.
Related JoVE Video
Dynamic gene expressions of peripheral blood mononuclear cells in patients with acute exacerbation of chronic obstructive pulmonary disease: a preliminary study.
Crit Care
PUBLISHED: 04-28-2014
Show Abstract
Hide Abstract
IntroductionAcute exacerbation of chronic obstructive pulmonary disease (AECOPD) is a serious event, responsible for the progress of the disease, increases in medical costs, and high mortality.MethodsThe present study aimed to identify AECOPD-specific biomarkers by evaluating the dynamic gene profiling of peripheral blood mononuclear cells (PBMCs) from patients with AECOPD on days 1, 3 and 10 after hospital admission, to compare with healthy controls or patients with stable COPD.ResultsWe found that 14 genes were co-differentially upregulated and 2 downregulated over 10-fold in patients with COPD or AECOPD, compared with the healthy individuals. Eight co-differentially upregulated genes and six downregulated genes were identified as a panel of AECOPD-specific genes. Downregulation of TCF7 in PBMCs was found to be associated with the severity of COPD. Dynamic changes of Aminolevulinate-delta-synthase 2 and carbonic anhydrase I had similar patterns of Digital Evaluation Score System and may serve as potential genes during the course of AECOPD.ConclusionThus, our findings indicate a panel of altered gene expression patterns in PBMCs can be used as AECOPD-specific dynamic biomarkers to monitor the course of the disease.
Related JoVE Video
Percutaneous Transhepatic Biliary Metal Stent for Malignant Hilar Obstruction: Results and Predictive Factors for Efficacy in 159 Patients from a Single Center.
Cardiovasc Intervent Radiol
PUBLISHED: 04-01-2014
Show Abstract
Hide Abstract
To investigate and compare the efficacy and safety of percutaneous transhepatic biliary stenting (PTBS) using a one- or two-stage procedure and determine the predictive factors for the efficacious treatment of malignant hilar obstruction (MHO).
Related JoVE Video
Neuroprotection of microRNA in neurological disorders (Review).
Biomed Rep
PUBLISHED: 03-25-2014
Show Abstract
Hide Abstract
MicroRNAs (miRNAs) are small, endogenous, non-coding RNA molecules that function as post-transcriptional regulators of gene expression by imperfect base-pairing with the 3'-untranslated regions of their target mRNAs. Altered expression of numerous miRNAs has been shown to be extensively involved in the pathogenesis of various diseases and cancers. Additionally, the specific expression of miRNAs in the nervous system has indicated that miRNAs are critical for the occurrence and development of neurological diseases. Increasing evidence has shown that specific miRNAs target the expression of particular proteins that are significant in the disease pathogenesis. Therefore, miRNA-mediated regulation may be important in the occurrence and development of neurological diseases and may function as a novel biomarker and tool for clinical therapy. In the present study, the significance of miRNAs is reviewed in a number of neurological disorders and the possibility of their use in therapeutic interventions is evaluated.
Related JoVE Video
Identification of Conserved and Novel microRNAs in Cerebral Ischemia-Reperfusion Injury of Rat Using Deep Sequencing.
J. Mol. Neurosci.
PUBLISHED: 03-24-2014
Show Abstract
Hide Abstract
MicroRNAs are a class of noncoding small RNAs that regulate gene expression by inhibiting target genes at post-transcriptional levels. MicroRNAs have been highlighted in many organs and tissues, including the brain. To identify special microRNAs involved in ischemia-reperfusion injury, we performed a comprehensive small RNA profiling in rat model and the control using Illumina high-throughput sequencing. A total of 9,444,562 and 10,290,391 clean reads were sequenced from two small RNA libraries constructed, respectively. Three hundred fifty-eight known microRNAs were identified, in which 78 microRNAs exhibited significantly differential expression between model and control. In addition, 62 and 68 novel miRNAs were found in model and control, respectively. Comparative analysis showed that 24 novel microRNAs were differentially expressed with greater than six-fold change. The GO annotation suggested that predicted targets of microRNAs were enriched into the category of metabolic process, cell part, cell-extracellular communications, and so on. KEGG pathway analysis suggested that these genes were involved in many important pathways, mainly including signaling transduction, MAPK signaling pathway, NF-?B signaling pathway, and neurotrophin signaling pathway. Our findings provided a deeper understanding to the regulatory mechanism of microRNAs underlying cerebral ischemia, therefore benefitting the improvement of the protection and treatment strategies of this disease.
Related JoVE Video
Heterodimerization of human apelin and bradykinin 1 receptors: novel signal transduction characteristics.
Cell. Signal.
PUBLISHED: 02-28-2014
Show Abstract
Hide Abstract
Apelin receptor (APJ) and bradykinin 1 receptor (B1R) are involved in a variety of important physiological processes, which share many similar characteristics in distribution and functions in the cardiovascular system. This study explored the possibility of heterodimerization between APJ and B1R, and investigated the impact of heterodimer on the signal transduction characteristics and the physiological functions in human endothelial cells after stimulation with their agonists. We first identified the endogenous expression of APJ and B1R in HUVECs and their co-localization on HEK293 membrane. The constitutive heterodimerization between the APJ and B1R was then demonstrated by BRET and FRET assays. Stimulation with Apelin-13 and des -Arg(9)-BK enhanced the phosphorylation of eNOS in HUVECs, which could be dampened by the knockdown of APJ or B1R, indicating the co-existence of APJ and B1R is critical for eNOS phosphorylation in HUVECs. Furthermore, APJ/B1R heterodimers were found to enhance the activity of PKC signaling pathway and increase intracellular Ca(2+) concentration in HEK293 cells, which might be the mechanism of APJ/B1R heterodimers promoting the phosphorylation of eNOS and leads to increased G?q, PKC signal pathway activities and a significant increase in cell proliferation. The results provide a new theoretical and experimental base for revealed intracellular molecular mechanisms of physiological function involved in the APJ and B1R and provide potential new targets for the development of drugs and treating cardiovascular disease.
Related JoVE Video
Chinese water-pipe smoking and the risk of COPD.
Chest
PUBLISHED: 02-22-2014
Show Abstract
Hide Abstract
Studies show that the incidence of COPD has remained high in southwest China despite the 1976 National Stove Improvement Program for indoor air quality. Chinese water-pipe tobacco smoking (commonly referred to as water-pipe smoking), which is thought to be less harmful under the assumption that no charcoal is used and water filters tobacco smoke, is popular in China. We investigated whether Chinese water-pipe use and exposure are associated with the risk of COPD.
Related JoVE Video
Keratinocyte growth factor-2 is protective in lipopolysaccharide-induced acute lung injury in rats.
Respir Physiol Neurobiol
PUBLISHED: 02-17-2014
Show Abstract
Hide Abstract
Keratinocyte growth factor-2 (KGF-2) plays a key role in lung development, but its role in acute lung injury has not been well characterized. Lipopolysaccharide instillation caused acute lung injury, which significantly elevated lung wet-to-dry weight ratio, protein and neutrophils in bronchoalveolar lavage fluid (BALF), inhibited surfactant protein A and C expression in lung tissue, and increased pathological injury. Pretreatment with KGF-2 improved the above lung injury parameters, partially restored surfactant protein A and C expression, and KGF-2 given 2-3 days before LPS challenge showed maximum lung injury improvement. Pretreatment with KGF-2 also markedly reduced the levels of TNF-?, MIP-2, IL-1? and IL-6 in BALF and the levels of IL-1? and IL-6 in lung tissue. Histological analysis showed there was increased proliferation of alveolar type II epithelial cells in lung parenchyma, which reached maximal 2 days after KGF-2 instillation. Intratracheal administration of KGF-2 attenuates lung injury induced by LPS, suggesting KGF-2 may be potent in the intervention of acute lung injury.
Related JoVE Video
Adrenaline stimulates the proliferation and migration of mesenchymal stem cells towards the LPS-induced lung injury.
J. Cell. Mol. Med.
PUBLISHED: 02-14-2014
Show Abstract
Hide Abstract
Bone marrow-derived mesenchymal stem cells (BMSCs) could modulate inflammation in experimental lung injury. On the other hand, adrenergic receptor agonists could increase DNA synthesis of stem cells. Therefore, we investigated the therapeutic role of adrenaline-stimulated BMSCs on lipopolysaccharide (LPS)-induced lung injury. BMSCs were cultured with adrenergic receptor agonists or antagonists. Suspensions of lung cells or sliced lung tissue from animals with or without LPS-induced injury were co-cultured with BMSCs. LPS-stimulated alveolar macrophages were co-cultured with BMSCs (with adrenaline stimulation or not) in Transwell for 6 hrs. A preliminary animal experiment was conducted to validate the findings in ex vivo study. We found that adrenaline at 10 ?M enhanced proliferation of BMSCs through both ?- and ?-adrenergic receptors. Adrenaline promoted the migration of BMSCs towards LPS-injured lung cells or lung tissue. Adrenaline-stimulated BMSCs decreased the inflammation of LPS-stimulated macrophages, probably through the expression and secretion of several paracrine factors. Adrenaline reduced the extent of injury in LPS-injured rats. Our data indicate that adrenaline-stimulated BMSCs might contribute to the prevention from acute lung injury through the activation of adrenergic receptors, promotion of proliferation and migration towards injured lung, and modulation of inflammation.
Related JoVE Video
Pulmonary cryptococcosis with trachea wall invasion in an immunocompetent patient: a case report and literature review.
Respiration
PUBLISHED: 02-13-2014
Show Abstract
Hide Abstract
Cryptococcosis causes significant morbidity and mortality in the world. Pulmonary cryptococcosis is a kind of subacute or chronic pulmonary fungal disease. We present a case of pulmonary cryptococcosis with a trachea wall invasion-like malignant tumor in an immunocompetent patient and a literature review. The 44-year-old man, a nonsmoker, suffered from mild dyspnea and white sputum with intermittent blood streaks. A computed tomography (CT) scan of his chest showed two possibly malignant lesions in the right hilum and upper-right field of his lung, which have higher uptake values of fluorodeoxyglucose on positron emission tomography (PET)/CT. Lung biopsy pathology showed scattered fungal spores and positive periodic acid-Schiff (PAS) staining. The immune status and blood tumor markers were all normal in this patient. The titer of Cryptococcus antigen latex agglutination test was 1:1,280. Under fiberoptic bronchoscopy, a prominent new mass on the right wall of the trachea blocked most of the right main bronchus. To reduce the symptoms of airway obstruction, treatment by bronchoscopy, i.e. ablation and endotracheal stent, was used. As his symptoms were aggravated by the use of itraconazole, amphotericin B liposome was used as antifungal treatment. All these methods led to a better prognosis. We conclude that pulmonary cryptococcosis may mimic lung neoplasms radiologically and bronchoscopically, even in immunocompetent patients.
Related JoVE Video
Endoplasmic reticulum stress in cerebral ischemia.
Neurochem. Int.
PUBLISHED: 02-03-2014
Show Abstract
Hide Abstract
The endoplasmic reticulum (ER) stress is an essential step in the progression of brain ischemia/reperfusion (I/R) injury. It is possible that the timing of events for ER stress signaling regulation is important for the balance of life and death such that ER stress is initially protective, aiming to restore ER homeostasis, whereas prolonged periods of ER stress can be deleterious and damaging. Nevertheless, modulation of ER stress exerts a remarkable protective effect on the ischemic brain and offers the prospect of new stroke therapies. As ER stress is not devoid of deleterious side effects, a better understanding of the reciprocal interaction between the ER and the ischemic brain is essential to harness the full therapeutic potential of ischemic stroke.
Related JoVE Video
Keratinocyte growth factor-2 intratracheal instillation significantly attenuates ventilator-induced lung injury in rats.
J. Cell. Mol. Med.
PUBLISHED: 01-28-2014
Show Abstract
Hide Abstract
Preservation or restoration of normal alveolar epithelial barrier function is crucial for pulmonary oedema resolution. Keratinocyte growth factor-2 (KGF-2), a potent epithelial cell mitogen, may have a role in preventing ventilator-induced lung injury (VILI), which occurs frequently in mechanically ventilated patients. The aim of the study was to test the role of KGF-2 in VILI in rats. Forty healthy adult male Sprague-Dawley rats were randomly allocated into four groups, where rats in Groups HVZP (high-volume zero positive end-expiratory pressure) and HVZP+KGF-2 were given intratracheally equal PBS and 5 mg/kg KGF-2 72 hrs before 4 hrs HVZP ventilation (20 ml/kg), respectively, while PBS and KGF-2 were administered in the same manner in Groups Control and KGF-2, which underwent tracheotomy only with spontaneous breathing. Inflammatory cytokines (tumour necrosis factor-?, macrophage inflammatory protein 2), neutrophil and total protein levels in bronchoalveolar lavage fluid and surfactant protein mRNA expression in lung tissue were detected; the number of alveolar type II cells, lung water content and lung morphology were also evaluated. The results indicate that pre-treatment with KGF-2 showed dramatic improvement in lung oedema and inflammation compared with HVZP alone, together with increased surfactant protein mRNA and alveolar type II cells. Our results suggest that KGF-2 might be considered a promising prevention for human VILI or other acute lung injury diseases.
Related JoVE Video
Protective effects of keratinocyte growth factor-2 on ischemia-reperfusion-induced lung injury in rats.
Am. J. Respir. Cell Mol. Biol.
PUBLISHED: 01-24-2014
Show Abstract
Hide Abstract
Ischemia-reperfusion (I/R) is a common cause to compromise tissue injury via endothelial and epithelial barrier dysfunction and damage. Keratinocyte growth factor (KGF)-2 could play an important role in the repair of alveolar epithelial damage and maintain the capillary barrier function. The present study aimed to investigate the potential effects of KGF-2 on I/R-induced lung injury in rats and the related mechanisms. KGF-2 (2.5-10 mg/kg) was administered intratracheally in rats 3 days before the left lobe with ischemia for 60 minutes followed by reperfusion for 180 minutes. Lung injury was evaluated by measuring lung morphology, blood gas analysis, total cell number, and protein concentration in the bronchoalveolar lavage fluid. The protective effects of KGF-2 on human pulmonary microvascular endothelial cells and related mechanisms were evaluated. Pretreatment with KGF-2 significantly prevented I/R-induced lung edema, inflammatory cell infiltration, protein exudation, and the release of inflammatory cytokines in rats, or I/R-induced endothelial cell apoptosis, migration, and barrier dysfunction. Phosphoinositide 3-kinase or epidermal growth factor receptor inhibitors attenuated the protective effect of KGF-2 in endothelial cells. Our results evidence that the local administration of KGF-2 may be an alternative to prophylactic or adjunct drug therapies for I/R-induced lung injury.
Related JoVE Video
Nicotine gum or patch treatment for smoking cessation and smoking reduction: a multi-centre study in Chinese physicians.
Front Med
PUBLISHED: 01-23-2014
Show Abstract
Hide Abstract
In China, around 23% of physicians (41% male, 3% female) currently smoke. Pharmacotherapy for tobacco dependence is available, but is not widely used in China. The purpose of this study was to estimate the effectiveness and the safety on smoking cessation of nicotine gum and nicotine patch in Chinese healthcare professionals. Three hundred regular smokers motivated to quit were recruited from six hospitals in China. All subjects were accepted nicotine replacement therapy, and they could choose nicotine gum (2 mg or 4 mg, depending on baseline smoking level) or nicotine patch (15 mg/16 h) for 12 weeks, with a 12-week follow-up. Limited behavioural support was provided. At Week 24, the 2-24 weeks continuous abstinence rate (verified by expired carbon monoxide) was 17%, the point prevalence abstinence rate (no smoking since the previous visit) was 35%, and 38% of subjects had continuously reduced their daily cigarette consumption by at least 50% versus baseline. Compliance with treatment was good, particularly with patch. No serious adverse event was reported, and most adverse events were mild or moderate. The most common treatment-related adverse events were gastrointestinal (both gum and patch) and local irritation symptoms. Nicotine patch and gum were well tolerated in Chinese smokers. Abstinence rates were comparable to those previously reported with nicotine replacement therapy, and many smokers who did not quit substantially reduced their cigarette consumption.
Related JoVE Video
EVS vs TIPS shunt for gastric variceal bleeding in patients with cirrhosis: A meta-analysis.
World J Gastrointest Pharmacol Ther
PUBLISHED: 01-22-2014
Show Abstract
Hide Abstract
To evaluate the clinical effects of transjugular intrahepatic portosystemic shunt (TIPS) vs endoscopic variceal sclerotherapy (EVS) in the management of gastric variceal (GV) bleeding in terms of variceal rebleeding, hepatic encephalopathy (HE), and survival by meta-analysis.
Related JoVE Video
Validation and target gene screening of hsa-miR-205 in lung squamous cell carcinoma.
Chin. Med. J.
PUBLISHED: 01-21-2014
Show Abstract
Hide Abstract
Lung cancers are classified as squamous cell carcinoma (SQ), adenocarcinoma (AC) and small cell lung carcinoma (SCLC). SQ is the major subtype of lung cancer. Currently, there are no targeted therapies for SQ due to lack of understanding its driving oncogenes. In this study, we validated an SQ specific biomarker hsa-miR-205 in Chinese patients with lung cancer and screened its candidate target genes for further functional studies to enrich knowledge in SQ target therapies.
Related JoVE Video
Selection of a TIPS stent for management of portal hypertension in liver cirrhosis: an evidence-based review.
World J. Gastroenterol.
PUBLISHED: 01-18-2014
Show Abstract
Hide Abstract
Nowadays, transjugular intrahepatic portosystemic shunt (TIPS) has become a mainstay treatment option for the management of portal hypertension-related complications in liver cirrhosis. Accumulated evidence has shown that its indications are being gradually expanded. Notwithstanding, less attention has been paid for the selection of an appropriate stent during a TIPS procedure. Herein, we attempt to review the current evidence regarding the diameter, type, brand, and position of TIPS stents. Several following recommendations may be considered in the clinical practice: (1) a 10-mm stent may be more effective than an 8-mm stent for the management of portal hypertension, and may be superior to a 12-mm stent for the improvement of survival and shunt patency; (2) covered stents are superior to bare stents for reducing the development of shunt dysfunction; (3) if available, Viatorr stent-grafts may be recommended due to a higher rate of shunt patency; and (4) the placement of a TIPS stent in the left portal vein branch may be more reasonable for decreasing the development of hepatic encephalopathy. However, given relatively low quality of evidence, prospective well-designed studies should be warranted to further confirm these recommendations.
Related JoVE Video
Nonselective ?-blockers may induce development of portal vein thrombosis in cirrhosis.
World J. Gastroenterol.
PUBLISHED: 01-04-2014
Show Abstract
Hide Abstract
Currently, nonselective ?-blockers (NSBBs) are commonly used for the prevention of variceal bleeding in liver cirrhosis. The beneficial effects of NSBBs are primarily attributed to the reduction in cardiac output by blockade of ?1 receptors and vasoconstriction of the splanchnic circulation by the blockade of ?2 receptors. The prognostic value of occlusive portal vein thrombosis (PVT) in cirrhotic patients has been increasingly recognized. The most important risk factor for the development of PVT in liver cirrhosis is the decreased portal vein inflow velocity. Collectively, we propose that the use of NSBBs potentially increases the development of portal vein thrombosis by reducing portal vein inflow velocity. The hypothesis should be confirmed by prospective cohort studies, in which cirrhotic patients without prior PVT treated with and without NSBBs are enrolled, and the development of PVT during follow-up is compared between the two groups. Additionally, subgroup analyses should be performed according to the dosage of NSBBs and the reduction of portal inflow velocity after use of NSBBs.
Related JoVE Video
Proteomic profiling of lymphocytes in autoimmunity, inflammation and cancer.
J Transl Med
PUBLISHED: 01-04-2014
Show Abstract
Hide Abstract
Lymphocytes play important roles in the balance between body defense and noxious agents involved in a number of diseases, e.g. autoimmune diseases, allergic inflammation and cancer. The proteomic analyses have been applied to identify and validate disease-associated and disease-specific biomarkers for therapeutic strategies of diseases. The proteomic profiles of lymphocytes may provide more information to understand their functions and roles in the development of diseases, although proteomic approaches in lymphocytes are still limited. The present review overviewed the proteomics-based studies on lymphocytes to headlight the proteomic profiles of lymphocytes in diseases, such as autoimmune diseases, allergic inflammation and cancer, with a special focus on lung diseases. We will explore the potential significance of diagnostic biomarkers and therapeutic targets from the current status in proteomic studies of lymphocytes and discuss the value of the currently available proteomic methodologies in the lymphocytes research.
Related JoVE Video
Edaravone protected PC12 cells against MPP(+)-cytoxicity via inhibiting oxidative stress and up-regulating heme oxygenase-1 expression.
J. Neurol. Sci.
PUBLISHED: 01-03-2014
Show Abstract
Hide Abstract
Oxidative stress is involved in the pathogenesis of Parkinson's disease (PD). Edaravone has been shown to have a neuroprotective effect. In the present work, we investigated the effect of edaravone on 1-methyl-4-phenylpyridinium (MPP(+))-treated PC12 cells. Edaravone inhibited the decrease of cell viability and apoptosis induced by MPP(+) in PC12 cells. In addition, edaravone alleviated intracellular reactive oxygen species (ROS) production. MPP(+) induced heme oxygenase-1 (HO-1) expression, which was further enhanced by edaravone. The inhibitor of HO-1 zinc protoporphyrin-IX attenuated the neuroprotection of edaravone. So edaravone protected PC12 cells against MPP(+)-cytoxicity via inhibiting oxidative stress and up-regulating HO-1 expression. The data showed that edaravone was neuroprotective and could be potentially therapeutics for PD in future.
Related JoVE Video
PcrV antibody protects multi-drug resistant Pseudomonas aeruginosa induced acute lung injury.
Respir Physiol Neurobiol
PUBLISHED: 01-03-2014
Show Abstract
Hide Abstract
Blocking PcrV, an essential component of the Type III secretion system (TTSS), has demonstrated efficacy against Pseudomonas aeruginosa infections. However, most of the results came from laboratory strains. Whether it is applicable to clinically isolated multi-drug resistant (MDR) strains is unknown. In this study we investigated the expression level of TTSS in clinically isolated MDR P. aeruginosa strains and the effects of anti-PcrV antibody on MDR isolate induced acute lung injury (ALI). The expression level of TTSS was quantified in 53 isolates including 25 MDR strains and 28 susceptible strains. We investigated the effect of anti-PcrV antibody through a murine model induced by instillation of a MDR strain into the left lung through trachea. Our results showed that the expression level of TTSS in MDR strains is comparable to susceptible strains. Anti-PcrV ensured the survival of challenged mice, reduced the bacteria numbers and attenuated lung inflammation and injury. This study proved that anti-PcrV may be a potentially effective strategy against MDR P. aeruginosa induced ALI.
Related JoVE Video
Association of single nucleotide polymorphisms of nucleotide excision repair genes with laryngeal cancer risk and interaction with cigarette smoking and alcohol drinking.
Tumour Biol.
PUBLISHED: 01-02-2014
Show Abstract
Hide Abstract
We investigated the potential association of 12 single nucleotide polymorphisms (SNPs) of nucleotide excision repair (NER) genes with risk of laryngeal cancer. A ratio of 1:1 matched case-control study was conducted. Conditional regression analysis indicated that subjects with ERCC1 rs11615 CC and C allele had an increased risk of laryngeal cancer compared with the TT genotype. Individuals with the ERCC5 rs17655 GG and G allele had a moderately increased risk of laryngeal cancer when compared with the CC genotype. By stratified analysis, ERCC1 rs11615 CC genotype and C allele were significantly associated with greatly increased risk of laryngeal cancer in ever smokers. ERCC1 rs11615 and ERCC5 rs17655 polymorphisms were associated with a moderately increased risk of this cancer in ever drinkers. In summary, we suggest that genetic variations in ERCC1 rs11615 and ERCC5 rs17655 are associated with laryngeal cancer risk in a Chinese population, especially in ever smokers and drinkers. Our finding could be helpful in identifying people at high risk for the disease for early intervention.
Related JoVE Video
Low Serum Cartonectin/CTRP3 Concentrations in Newly Diagnosed Type 2 Diabetes Mellitus: In Vivo Regulation of Cartonectin by Glucose.
PLoS ONE
PUBLISHED: 01-01-2014
Show Abstract
Hide Abstract
Cartonectin is a novel adipokine of the C1q complement/TNF-related protein (CTRP) superfamily, with glucose lowering effects, anti-inflammatory and cardio-protective properties. We sought to investigate circulating cartonectin concentrations in subjects with type 2 diabetes mellitus (T2DM) as well as age and BMI matched control subjects. We also examined the effects of a 2 hour 75 g oral glucose tolerance test (OGTT) on serum cartonectin concentrations in T2DM subjects.
Related JoVE Video
A taxonomic review on the species of Tetraserica Ahrens, 2004, of China (Coleoptera, Scarabaeidae, Sericini).
Zookeys
PUBLISHED: 01-01-2014
Show Abstract
Hide Abstract
A review on the Chinese species of Tetraserica Ahrens, 2004, is presented. The lectotype of Tetrasericatonkinensis (Moser, 1908), comb. n. is designated. Twenty-nine new Tetraserica species are described from China and adjacent regions: Tetrasericaanhuaensis sp. n., Tetrasericachangjiangensis sp. n., Tetrasericachangshouensis sp. n., Tetrasericadamaidiensis sp. n., Tetrasericadaqingshanica sp. n., Tetrasericafikaceki sp. n., Tetrasericagraciliforceps sp. n., Tetrasericajinghongensis sp. n., Tetrasericaleishanica sp. n., Tetrasericaliangheensis sp. n., Tetrasericalinaoshanica sp. n., Tetrasericalongipenis sp. n., Tetrasericalongzhouensis sp. n., Tetrasericamaoershanensis sp. n., Tetrasericamengeana sp. n., Tetrasericamenglongensis sp. n., Tetrasericapingjiangensis sp. n., Tetrasericaruiliana sp. n., Tetrasericaruiliensis sp. n., Tetrasericasculptilis sp. n., Tetrasericashangsiensis sp. n., Tetrasericashunbiensis sp. n., Tetrasericasigulianshanica sp. n., Tetrasericatianchiensis sp. n., Tetrasericawandingensis sp. n., Tetrasericawangtongensis sp. n., Tetrasericaxichouensis sp. n., Tetrasericayaoanica sp. n., Tetrasericayaoquensis sp. n. A key to the Chinese Tetraserica species is given, species distribution as well as the habitus and male genitalia of all species are illustrated.
Related JoVE Video
A taxonomic revision of the Neoserica(sensu lato)calva group (Coleoptera, Scarabaeidae, Sericini).
Zookeys
PUBLISHED: 01-01-2014
Show Abstract
Hide Abstract
The species of the Neoserica(sensu lato)calva group are revised. Neosericacalva Frey, 1972, comb. n. is redescribed. Thirteen new species are described from China and South Korea: Neosericaailaoshanica sp. n., Neosericaanonyma sp. n., Neosericacalvoides sp. n., Neosericagulinqingensis sp. n., Neosericakoelkebecki sp. n., Neosericaliangi sp. n., Neosericaluxiensis sp. n., Neosericamenghaiensis sp. n., Neosericamengi sp. n., Neosericataipingensis sp. n., Neosericazheijangensis sp. n., Neosericazhibenshanica sp. n., and Neosericazongyuani sp. n. A key to Sericini genera with multilamellate antenna and species groups of Neoserica of mainland Asia as well as a key to species of the Neosericacalva group are provided. A map of species distribution is given, habitus and male genitalia are illustrated.
Related JoVE Video
A taxonomic revision of the Neoserica (sensu lato) pilosula group (Coleoptera, Scarabaeidae, Sericini).
Zookeys
PUBLISHED: 01-01-2014
Show Abstract
Hide Abstract
Nine new species of the Neoserica (sensu lato) pilosula Moser, 1915, group are described from China: Neoserica curvipenis sp. n., N. emeishanensis sp. n., N. lincangensis sp. n., N. ludingensis sp. n., N. lushuiana sp. n., N. rangshuiensis sp. n., N. shennongjiaensis sp. n., N. tianeana sp. n., and N. weibaoshanica sp. n. The lectotype of Neoserica pilosula Moser, 1915, is designated. Habitus and male genitalia are illustrated, a key to the species of the group and a map of species distribution are given.
Related JoVE Video
A taxonomic review of the Neoserica (sensu lato) abnormis group (Coleoptera, Scarabaeidae, Sericini).
Zookeys
PUBLISHED: 01-01-2014
Show Abstract
Hide Abstract
The present paper revises the species belonging to the Neoserica (sensu lato) abnormis group, so far known only with two nominal species. Twenty new species are herein described from Indochina and southern China: N. abnormoides sp. n. (Vietnam, China), N. allolaotica sp. n., N. namthaensis sp. n., N. simplicissima sp. n. (Laos), N. thailandensis sp. n. (Thailand), N. alloputaoana sp. n., N. kanphantensis sp. n., N. natmatoungensis sp. n., N. putaoana sp. n., N. taunggyiana sp. n. (Myanmar), N. lamellosa sp. n., N. tonkinea sp. n. (Vietnam), N. bairailingshanica sp. n., N. euyunnanica sp. n., N. huangi sp. n., N. jiangxiensis sp. n., N. trifida sp. n., N. yaoi sp. n., N. yingjiangensis sp. n. (China), N. cardamomensis sp. n. (Indochina and southern China). One new combination is established: Neoserica ponderosa Arrow, 1946, comb. n. The lectotypes of Neoserica abnormis Moser, 1908 and the taxonomically uncertain N. inclinata Brenske, 1898, which very likely also belongs to this species group, are designated herein. A key to the species and to species groups is given, the genitalia of all species including their habitus are illustrated. Maps of species distribution are included.
Related JoVE Video
Lavage with allicin in combination with vancomycin inhibits biofilm formation by Staphylococcus epidermidis in a rabbit model of prosthetic joint infection.
PLoS ONE
PUBLISHED: 01-01-2014
Show Abstract
Hide Abstract
The present anti-infection strategy for prosthetic joint infections (PJI) includes the use of antibiotics and surgical treatments, but the bacterial eradication rates are still low. One of the major challenges is the formation of biofilm causing poor bacterial eradication. Recently it has been reported that allicin (diallyl thiosulphinate), an antibacterial principle of garlic, can inhibit bacteria adherence and prevent biofilm formation in vitro. However, whether allicin could inhibit biofilm formation in vivo is unknown. The aim of this study was to investigate the effects of allicin on biofilm formation, and whether allicin could potentiate the bactericidal effect of vancomycin in a rabbit PJI model.
Related JoVE Video
The prognostic value of 18F-FDG PET/CT for hepatocellular carcinoma treated with transarterial chemoembolization (TACE).
Theranostics
PUBLISHED: 01-01-2014
Show Abstract
Hide Abstract
18F-Fluoro-deoxyglucose (FDG) PET/CT can be used to monitor the biological behavior of hepatocellular carcinoma (HCC). Baseline PET/CT has prognostic value in HCC patients, but there is litter knowledge of the PET/CT changes after treatment. We evaluated 27 HCC patients treated with transarterial chemoembolization (TACE) from June 2011 to July 2012, and we investigated the prognostic value of PET/CT. Patients were followed up with regular clinical and laboratory examinations and contrast-enhanced spiral computed tomography (CT). Furthermore, PET/CT assessments were collected and analyzed before (range 1~15 d) and after the first month of TACE (range, 27~45 d). We tested the prognostic value of the tumor standardized uptake value (TSUV) and normal liver SUV(LSUV) according to the VOI (volume of interest). The SUVs were used to assess the relationship between the treatment response and survival. To assess their prognostic value, we evaluated the areas under the receiver operating characteristic (ROC) curves of different SUVs for predicting survival. Finally, the median overall survival (OS) and time to progression (TTP) for 27 patients were 15.4 months (95%CI, 3.3-27.5 months) and 11.4 months (95%CI, 6.7-16.1 months), respectively. The ?TSUVmax%, based on the VOI, had the highest discriminative prognostic value and the cutoff PET/CT response was 0.1 with a sensitivity of 100% and a specificity of 95.2%. The OS was significantly better in the PET/CT response group than in the PET/CT non-response group (p=0.025). In conclusion, an early interim PET/CT after TACE may have prognostic value for HCC patients treated with TACE, and the ?TSUVmax% may help in determining the HCCs viability in patients with high baseline and follow-up18F-FDG uptake.
Related JoVE Video
A taxonomic review of the Neoserica (sensu lato) septemlamellata group (Coleoptera, Scarabaeidae, Sericini).
Zookeys
PUBLISHED: 01-01-2014
Show Abstract
Hide Abstract
In the present paper the species belonging to the Neoserica (sensu lato) septemlamellata group, that included so far only four known species, are revised. Here we describe eleven new species originating mainly from Indochina and Southern China: N. daweishanica sp. n., N. gaoligongshanica sp. n., N. guangpingensis sp. n., N. igori sp. n., N. jiulongensis sp. n., N. plurilamellata sp. n., N. weishanica sp. n., N. yanzigouensis sp. n. (China) N. sapaensis sp. n. (China, Vietnam), N. bansongchana sp. n., N. takakuwai sp. n. (Laos). The lectotypes of Neoserica septemlamellata Brenske, 1898 and N. septemfoliata Moser, 1915 are designated. Keys to the species and species groups are given, the genitalia of all species and their habitus are illustrated and distribution maps are included.
Related JoVE Video
Meat consumption and risk of oral cavity and oropharynx cancer: a meta-analysis of observational studies.
PLoS ONE
PUBLISHED: 01-01-2014
Show Abstract
Hide Abstract
High meat consumption, especially red and processed meat consumption is associated with an increased risk of several cancers, however, evidence for oral cavity and oropharynx cancer is limited. Thus, we performed this meta-analysis to determine the association between intakes of total meat, processed meat, red meat, and white meat, and the risk of oral cavity and oropharynx cancer.
Related JoVE Video
MAML2 rearrangement in primary pulmonary mucoepidermoid carcinoma and the correlation with FLT1 expression.
PLoS ONE
PUBLISHED: 01-01-2014
Show Abstract
Hide Abstract
Primary pulmonary mucoepidermoid carcinoma (PMEC) is an uncommon neoplasm with remarkable resemblance to mucoepidermoid carcinoma of the salivary glands. The latter has been shown to harbor t(11,19) resulting in MECT1-MAML2 fusion, which may be of diagnostic and prognostic values. However, the importance of such feature in PMEC has not been well studied.
Related JoVE Video
Prognosis of 18 H7N9 avian influenza patients in Shanghai.
PLoS ONE
PUBLISHED: 01-01-2014
Show Abstract
Hide Abstract
To provide prognosis of an 18 patient cohort who were confirmed to have H7N9 lung infection in Shanghai.
Related JoVE Video
Constitutive activation of AMPK ?1 in vascular endothelium promotes high-fat diet-induced fatty liver injury: role of COX-2 induction.
Br. J. Pharmacol.
PUBLISHED: 01-01-2014
Show Abstract
Hide Abstract
AMP-activated protein kinase (AMPK), an important regulator of energy metabolism, comprises three (?, ? and ?) subunits, each with a unique tissue distribution. As AMPK has a wide range of protein and gene targets, defining its role has been difficult. Here, we have studied a transgenic mouse model overexpressing the constitutively active ?1 subunit of AMPK in endothelial cells (EC-AMPK) to elucidate its role in energy homeostasis.
Related JoVE Video
[Flap repair for vascular prosthesis exposure after artificial blood vessel bypass surgery for critical limb ischemia].
Zhonghua Wai Ke Za Zhi
PUBLISHED: 11-21-2013
Show Abstract
Hide Abstract
To investigate the effectiveness of flap repair for vascular prosthesis exposure after the artificial blood vessel bypass surgery for critical limb ischemia.
Related JoVE Video
[Triptolide Inhibits Proliferation and Iuduces Apoptosis of Imatinib Resistant K562/G01 cells].
Zhongguo Shi Yan Xue Ye Xue Za Zhi
PUBLISHED: 10-26-2013
Show Abstract
Hide Abstract
This study was aimed to explore the inhibitory effect of triptolide on proliferation and inducing apoptosis effect of K562/G01 cells and their possible mechanism. MTT assay was used to detect the effect of imatinib or triptolide alone and their combination on K562/G01 proliferation; the cell cycle, apoptosis rate, P-gp protein expression were detected by folw cytometry (FCM); the expression of P-gp was assessed by Western blot; the BCR/ABL gene expression was assayed by real time guantitative PCR. The results showed that triptolide could enhance the effect of imatinib on proliferation inhibition and apoptosis of K562/G01, arrested the cell cycle in G1 phase, down-regulated the expression of BCR/ABL gene and P-gp protein. It is concluded that triptolide induces K562/G01 cell proliferation inhibition and apoptosis, the mechanism may be related to cell cycle arrest, decrease of P-gp protein expression, inhibition of BCR/ABL gene expression.
Related JoVE Video
The implication of telehealthcare in COPD management of China.
Expert Rev Respir Med
PUBLISHED: 10-22-2013
Show Abstract
Hide Abstract
Chronic obstructive pulmonary disease (COPD) is a very common disease all around the world and has become an increasing public health concern to the Chinese medical community. In the past decades, telehealthcare technology has become a good way to manage COPD but current evidence makes it hard to determine the effectiveness of this technology. Internet of things (Iot) is a recent breakthrough in communication technology, which links the virtual world to the real world through connection between sensors and working devices. It relates people and items in any ways so that data collection and management become more flexible. Our review concentrates on the effectiveness and potential application of telehealthcare in COPD management and how IoT technology may stimulate COPD healthcare delivery through telehealthcare technology.
Related JoVE Video
Curcumin induces autophagy via activating the AMPK signaling pathway in lung adenocarcinoma cells.
J. Pharmacol. Sci.
PUBLISHED: 09-18-2013
Show Abstract
Hide Abstract
Curcumin is a major yellow pigment and active component of turmeric widely used as dietary spice and herbal medicine. This compound has been reported to be a promising antitumor agent, although the underlying molecular mechanisms are not fully understood yet. In this study, we reported that curcumin inhibited growth of lung adenocarcinoma cells, but had no cytotoxic activity to IMR-90 normal lung fibroblast cells. Curcumin induced autophagy in the A549 human lung adenocarcinoma cell line, evidenced by LC3 immunofluorescence analysis and immunoblotting assays on LC3 and SQSTM1. Moreover, the autophagy inhibitor 3-MA partly blocked the inhibitory effect of curcumin on the growth of A549 cells. Curcumin markedly increased the phosphorylation of AMP-activated protein kinase (AMPK) and acetylCoA carboxylase in A549 cells. At last, pharmacological blockade of the AMPK signaling pathway by compound C and genetic disruption of the AMPK signaling pathway with siRNA-mediated AMPK?1 knockdown impaired the autophagy-inducing effect of curcumin. Collectively, our data suggests that curcumin induces autophagy via activating the AMPK signaling pathway and the autophagy is important for the inhibiting effect of curcumin in lung adenocarcinoma cells.
Related JoVE Video
The pretreatment platelet and plasma fibrinogen level correlate with tumor progression and metastasis in patients with pancreatic cancer.
Platelets
PUBLISHED: 09-03-2013
Show Abstract
Hide Abstract
Abstract Cancer patients frequently present with activated coagulation pathways and thrombocytosis, which are potentially associated with tumor progression and prognosis. However, the prognostic value of abnormal plasma fibrinogen and platelet levels for the treatment of pancreatic cancer is unclear. The purpose of our study was to evaluate the prognostic value of plasma fibrinogen and platelet levels in pancreatic cancer, and to devise a prognostic model to identify the patients with greatest risk for a poor overall survival. One hundred and twenty-five patients diagnosed with pancreatic ductal adenocarcinoma in our hospital between May 2000 and June 2005 were included in this study. The plasma fibrinogen and platelet levels were examined before treatment and analyzed along with patient clinicopathological parameters and overall survival. The foundation of prognostic model was based on the risk factors according to the Cox proportional hazard model. The incidence of hyperfibrinogenemia and thrombocytosis was 24.8% (31/125) and 15.2% (19/125), respectively. The mean fibrinogen concentration differed significantly between the early (I/II) and late (III/IV) stage patients (3.19?±?0.70 vs. 3.65?±?0.90?g/l, p?=?0.008). Patients with a higher concentration of plasma fibrinogen and platelets had a worse prognosis (p?
Related JoVE Video
New insights of aquaporin 5 in the pathogenesis of high altitude pulmonary edema.
Diagn Pathol
PUBLISHED: 08-31-2013
Show Abstract
Hide Abstract
High altitude pulmonary edema (HAPE) affects individuals and is characterized by alveolar flooding with protein-rich edema as a consequence of blood-gas barrier disruption. In this study, we hypothesized that aquaporin 5 (AQP5) which is one kind of water channels may play a role in preservation of alveolar epithelial barrier integrity in high altitude pulmonary edema (HAPE).
Related JoVE Video
Icotinib versus gefitinib in previously treated advanced non-small-cell lung cancer (ICOGEN): a randomised, double-blind phase 3 non-inferiority trial.
Lancet Oncol.
PUBLISHED: 08-13-2013
Show Abstract
Hide Abstract
Icotinib, an oral EGFR tyrosine kinase inhibitor, had shown antitumour activity and favourable toxicity in early-phase clinical trials. We aimed to investigate whether icotinib is non-inferior to gefitinib in patients with non-small-cell lung cancer.
Related JoVE Video
[The pharmacological effect of allosteric regulation at GPCR heterodimer].
Sheng Li Ke Xue Jin Zhan
PUBLISHED: 07-16-2013
Show Abstract
Hide Abstract
Traditionally GPCR can exist as monomer, and its function is linear. The signal is transduced when the ligand binds to the orthosteric site. It has been reported that GPCR can form homodimer or heterodimer. In the dimers, allosteric sites will form when ligand binds to one monomer and then regulates of another monomer. Therefore the signaling pathway and the function of the receptor will be changed. Compared to orthosteric agonists, allosteric modulators have a number of potential advantages, which make allosteric modulators as a drug discovery candidate for GPCR. Herein, we introduce the concept of allosteric regulation at GPCR dimers, and its impact on the function of the receptor. We also introduce the drug screening methods of allosteric regulator, and those will contribute to drug discovery for GPCR.
Related JoVE Video
Restrictive vs liberal transfusion for upper gastrointestinal bleeding: a meta-analysis of randomized controlled trials.
World J. Gastroenterol.
PUBLISHED: 07-09-2013
Show Abstract
Hide Abstract
To compare the outcome of upper gastrointestinal bleeding (UGIB) between patients receiving restrictive and liberal transfusion.
Related JoVE Video
Effects of phosphoinositide 3-kinase on protease-induced acute and chronic lung inflammation, remodeling, and emphysema in rats.
Chest
PUBLISHED: 07-09-2013
Show Abstract
Hide Abstract
Phosphoinositide 3-kinase (PI3K) plays an important role in tissue inflammatory reactions and fibrotic processes. The objective of this study was to evaluate the potential mechanism and therapeutic effects of PI3K inhibitor on pancreatic elastase (PE)-induced acute and chronic lung inflammation, edema, and injury.
Related JoVE Video
Prognostic factors in non-malignant and non-cirrhotic patients with portal cavernoma: An 8-year retrospective single-center study.
World J. Gastroenterol.
PUBLISHED: 06-06-2013
Show Abstract
Hide Abstract
To evaluate the outcome of non-malignant and non-cirrhotic patients with portal cavernoma and to determine the predictors for survival.
Related JoVE Video
Aprepitant triple therapy for the prevention of chemotherapy-induced nausea and vomiting following high-dose cisplatin in Chinese patients: a randomized, double-blind, placebo-controlled phase III trial.
Support Care Cancer
PUBLISHED: 05-22-2013
Show Abstract
Hide Abstract
Aprepitant, an oral neurokinin-1 receptor antagonist, has demonstrated improved control of chemotherapy-induced nausea and vomiting (CINV) in previous studies. This is the first phase III study to evaluate the efficacy and tolerability of aprepitant in patients receiving highly emetogenic chemotherapy (HEC) in Asian countries.
Related JoVE Video
Transjugular intrahepatic portosystemic shunt for Budd-Chiari syndrome: techniques, indications and results on 51 Chinese patients from a single centre.
Liver Int.
PUBLISHED: 05-15-2013
Show Abstract
Hide Abstract
In Western countries, transjugular intrahepatic portosytemic shunt (TIPS) is widely applied for the treatment of Budd-Chiari syndrome (BCS). However, the outcome of Chinese BCS patients treated with TIPS is extremely limited. Furthermore, the timing of conversion from percutaneous recanalization to TIPS remains uncertain.
Related JoVE Video
Loss-of-SIRT1 function during vascular ageing: Hyperphosphorylation mediated by cyclin-dependent kinase 5.
Trends Cardiovasc. Med.
PUBLISHED: 05-15-2013
Show Abstract
Hide Abstract
The longevity regulator SIRT1 is an enzyme catalyzing the deacetylation of protein substrates, in turn modulating their biological functions. In endothelial cells, downregulation of SIRT1 evokes cellular senescence. In aged arteries, SIRT1 expression and activity is blunted, which contributes to the development of atherosclerosis and abnormal vascular responses. A recent study suggests that cyclin-dependent kinase 5 (CDK5) is responsible for the phosphorylation of SIRT1 at the serine 47 residue. This modification blocks the anti-senescence activity of SIRT1 and plays a critical role in the loss-of-SIRT1 function during vascular ageing. Thus, by inhibiting CDK5, SIRT1 function can be improved, in turn preventing the development of atherosclerosis and slowing down the process of vascular ageing.
Related JoVE Video
Gene polymorphisms and chronic obstructive pulmonary disease.
J. Cell. Mol. Med.
PUBLISHED: 05-11-2013
Show Abstract
Hide Abstract
The genetic component was suggested to contribute to the development of chronic obstructive pulmonary disease (COPD), a major and growing public health burden. The present review aims to characterize the evidence that gene polymorphisms contribute to the aetiology of COPD and related traits, and explore the potential relationship between certain gene polymorphisms and COPD susceptibility, severity, lung function, phenotypes, or drug effects, even though limited results from related studies lacked consistency. Most of these studies were association studies, rather than confirmatory studies. More large-sized and strictly controlled studies are needed to prove the relationship between gene polymorphisms and the reviewed traits. More importantly, prospective confirmatory studies beyond initial association studies will be necessary to evaluate true relationships between gene polymorphisms and COPD and help individualized treatment for patients with COPD.
Related JoVE Video

What is Visualize?

JoVE Visualize is a tool created to match the last 5 years of PubMed publications to methods in JoVE's video library.

How does it work?

We use abstracts found on PubMed and match them to JoVE videos to create a list of 10 to 30 related methods videos.

Video X seems to be unrelated to Abstract Y...

In developing our video relationships, we compare around 5 million PubMed articles to our library of over 4,500 methods videos. In some cases the language used in the PubMed abstracts makes matching that content to a JoVE video difficult. In other cases, there happens not to be any content in our video library that is relevant to the topic of a given abstract. In these cases, our algorithms are trying their best to display videos with relevant content, which can sometimes result in matched videos with only a slight relation.