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Find video protocols related to scientific articles indexed in Pubmed.
[Analysis of XRD spectral characteristics of soil clay mineral in two typical cultivated soils].
Guang Pu Xue Yu Guang Pu Fen Xi
PUBLISHED: 10-02-2014
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The present paper took black soil and chernozem, the typical cultivated soil in major grain producing area of Northeast, as the study object, and determinated the soil particle composition characteristics of two cultivated soils under the same climate and location. Then XRD was used to study the composition and difference of clay mineral in two kinds of soil and the evolutionary mechanism was explored. The results showed that the two kinds of soil particles were composed mainly of the sand, followed by clay and silt. When the particle accumulation rate reached 50%, the central particle size was in the 15-130 microm interval. Except for black soil profile of Shengli Xiang, the content of clay showed converse sequence to the central particle in two soils. Clay accumulated under upper layer (18.82%) in black soil profile while under caliche layer (17.41%) in chernozem profile. Clay content was the least in parent material horizon except in black profile of Quanyanling. Analysis of clay XRD atlas showed that the difference lied in not only the strength of diffraction peak, but also in the mineral composition. The main contents of black soil and chernozem were both 2 : 1 clay, the composition of black soil was smectite/illite mixed layer-illite-vermiculite and that of chernozem was S/I mixture-illite-montmorillonite, and both of them contained little kaolinite, chlorite, quartz and other primary mineral. This paper used XRD to determine the characteristics of clay minerals comprehensively, and analyzed two kinds of typical cultivated soil comparatively, and it was a new perspective of soil minerals study.
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IL28B genetic variations are associated with treatment response of patients with chronic hepatitis C in Chinese Han population.
J Dig Dis
PUBLISHED: 09-30-2014
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This study aimed to investigate the association of interleukin 28B (IL28B) single nucleotide polymorphisms (SNPs) with sustained response (SVR) in Chinese Han patients with chronic hepatitis C (CHC) and to analysz the correlations between IL28B SNPs and their demographic, virological and clinical characteristics.
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Impact of methylene blue in addition to norepinephrine on the intestinal microcirculation in experimental septic shock.
Clin. Hemorheol. Microcirc.
PUBLISHED: 09-18-2014
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Methylene blue (MB) has been used with some success as a treatment for the vasoplegia of vasopressor-refractory septic shock. The putative mechanism of action of MB is the inhibition of endothelial nitric oxide within the microvasculature and improved responsiveness to endogenous catecholamines (norepinephrine (NE)). However, to date, no study has demonstrated the microcirculatory effect of methylene blue in septic shock. The objective of this randomized, controlled, animal study was to show, in an experimentally-induced, septic shock model in rats, the effects of MB and NE on global hemodynamics and the microcirculation. Mean arterial pressure (MAP) was drastically reduced following bacterial endotoxin (lipopolysaccharide, LPS) administration in animals not receiving vasopressors. Only the combination of NE + MB restored MAP to control levels by the end of the three hour experiment. Intravital microscopy of the microcirculation was performed in the terminal ileum in order to examine functional capillary density in intestinal muscle layers and the mucosa, as well as leukocyte activation in venules (rolling, adhesion to the endothelium). Untreated LPS animals showed a significant increase in leukocyte adhesion and a decrease in capillary perfusion in the intestinal microcirculation. In groups receiving NE or NE+MB, we observed a significant decrease in leukocyte adhesion and improved functional capillary density, indicating that microvasculature function was improved. This study suggests that methylene blue may be able to improve hemodynamics while preserving microvascular function in septic shock.
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Estrogen Decreases Anoikis of Ovarian Cancer Cell Line Caov-3 Through Reducing Release of Bit1.
DNA Cell Biol.
PUBLISHED: 09-12-2014
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Few studies have referred to the implication of anoikis processes following hormonal treatment. No data are available on the influence of estrogen in ovarian cancer anoikis. To gain insights into the effects and mechanism of estrogen in ovarian cancer cells, we have carried out studies on the anoikis of ovarian cancer cells treated with estrogen and on the pathways involved. We observed an anti-anoikis role of E2 in suspended Caov-3 cells, and this was mainly due to the decreasing of Bit1 level in cytosol. We also found that estrogen receptor ? (ER?) was the main mediator involved in this process. To study the signaling pathways well, phosphatidylinositol 3-kinase (PI3K)/AKT were further investigated. Results demonstrated that the decreasing of the Bit1 level in cytosol mediated by E2 binding to ER? was mainly through PI3K/AKT pathways. Overall, these findings disclose a new perspective for estrogen on ovarian cancer therapy.
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Ovarian Ablation Using Goserelin Improves Survival of Premenopausal Patients with Stage II/III Hormone Receptor-Positive Breast Cancer without Chemotherapy-Induced Amenorrhea.
Cancer Res Treat
PUBLISHED: 08-21-2014
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The purpose of this study was to assess the value of ovarian ablation using goserelin in premenopausal patients with stage II/III hormone receptor-positive breast cancer without chemotherapy-induced amenorrhea (CIA).
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[Quantitative determination of 5 active ingredients in different harvest periods of Ligusticum chuanxiong by HPLC].
Zhongguo Zhong Yao Za Zhi
PUBLISHED: 08-07-2014
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A simple and quick method is described for the determination of ferulic acid, senkyunolide I, senkyunolide H, senkyunolide A and ligustilide in rhizomes of Ligusticum chuanxiong. The 5 active ingredients in the sample was extracted using 40% ethanol and analyzed by reversed-phase high performance liquid chromatography (HPLC). Chromatography separation was performed using Agilent 1100 series HPLC system with a Symmetry C18 column and gradient elution with a mixture of three solvents : solvent A, acetonitrile, solvent B, methanol and solvent C, 1% aqueous acetic acid, 0 min to 5 min A: B: C 20: 40: 40, 5 min to 30 min A: B: C 60 to 100 : 0 : 40 to 0. The effluent was monitored using a VWD detector set at 321 nm (0-4.3 min) and 275 nm (4.31-30 min). The flow rate was set at 1 mL x min(-1) and the injection volume was 10 microL. The column temperature was maintained at 35 degrees C. The calibration curve was linear (r > or = 0.99) over the tested ranges. The average recovery was 94.44%-103.1% (n = 6). The method has been successfully applied to the analysis in different harvest periods of L. chuanxiong samples. In this paper, single-factor randomized block design to study the 5 components content of L. chuanxiong on ten collecting stages. For the L. chuanxiong collected from April 15th to May 30rd, the content of 5 ingredients increased primarily, and then decreased. Determine the appropriate harvest time has important significance to the promotion of the quality of L. chuanxiong.
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Benefit of post-mastectomy radiotherapy of the supra-/infraclavicular lymphatic drainage area in breast cancer patients.
Asian Pac. J. Cancer Prev.
PUBLISHED: 08-02-2014
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This study investigated the survival benefit of radiotherapy (RT) of the supra- and infraclavicular lymphatic drainage area in Chinese women with T1-2N1M0 breast cancer receiving mastectomy.
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[Cervical spondylosis of vertebral artery treated with thermosensitive moxibustion with different dosages: a randomized controlled trial].
Zhongguo Zhen Jiu
PUBLISHED: 07-16-2014
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To compare the difference in the clinical efficacy on cervical spondylosis of vertebral artery type (CSA) treated with thermosensitive moxibustion at different dosages.
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Tube-like ternary ?-Fe2O3@SnO2@Cu2O sandwich heterostructures: synthesis and enhanced photocatalytic properties.
ACS Appl Mater Interfaces
PUBLISHED: 07-11-2014
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Heterogeneous photocatalysis is of great interest for environmental remediation applications. However, fast recombination of photogenerated electron-hole pair and a low utilization rate of sunlight hinder the commercialization of currently available semiconductor photocatalysts. In this regard, we developed a unique ternary single core-double shell heterostructure that consists of ?-Fe2O3@SnO2@Cu2O. This heterostructure exhibits a tube-like morphology possessing broad spectral response for the sunlight due to the combination of narrow bandgap and wide bandgap semiconductors forming a p-n heterojunction. To fabricate such a short nanotube (SNT), we used an anion-assisted hydrothermal route for deposition of ?-Fe2O3, a seed-mediated deposition strategy for SnO2, and finally an aging process to deposit a Cu2O layer to complete the tube-like ternary ?-Fe2O3@SnO2@Cu2O single core-double shell heterostructures. The morphology, composition, and photocatalytic properties of those ternary core-shell-shell heterostructures were characterized by various analytical techniques. These ternary heterostructures exhibited enhanced photocatalytic properties on the photodegradation of the organic dye of Rhodamine B (RhB) under simulated sunlight irradiation. The origin of enhanced photocatalytic activity is due to the synergistic effect of broad spectral response by combining narrow bandgap and wide bandgap semiconductors and, hence, an efficient charge separation of photogenerated electron-hole pairs facilitated through the p-n heterojunction. Furthermore, our unique structure provides an insight on the fabrication and controlled preparation of multilayer heterostructural photocatalysts that have intriguing properties.
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Association between SNPs in miRNA-machinery genes and chronic hepatitis B in the Chinese Han population.
Infect. Genet. Evol.
PUBLISHED: 07-07-2014
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Single nucleotide polymorphisms (SNPs) in miRNA-machinery genes can influence their generation and maturation, then expression and structure. To explore the relationship between three SNPs (rs3757 in DGCR8, rs636832 in AGO1, rs7813 in GEMIN4) in miRNA-machinery genes and chronic hepatitis B, we genotyped the SNPs by high resolution melting method (HRM) in a case-control study of 332 unrelated chronic hepatitis B patients and 352 unrelated healthy controls in Western China. Interestingly, the rs636832 was significantly associated with the susceptibility of CHB (genotype: AA/GA/GG: p=0.010; allele: A/G: OR=0.727, 95% CI=0.575-0.920, p=0.008). The minor allele A of rs636832 was significantly associated with a decreased risk of CHB. Additionally, the dominant model AG+GG vs. AA showed a risk of 1.442-fold (p=0.018) with CHB. Further exploration for the association between rs636832 and HBV-DNA load in 329 cases showed no significant difference (genotype: p=0.321; allele: p=0.148). Neither did the association between rs636832 and the status of HBsAg and HbeAg (HBsAg: genotype p=0.337, allele p=0.436; HBeAg: genotype p=0.861, allele p=0.822). Our study first provided the evidence that rs636832 in AGO1 was associated with chronic HBV infection susceptibility in Chinese Han population. Further epidemiological and functional studies in larger populations are warranted to verify our results.
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One-pot controlled synthesis of sea-urchin shaped Bi2S3/CdS hierarchical heterostructures with excellent visible light photocatalytic activity.
Dalton Trans
PUBLISHED: 07-05-2014
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In this study, sea-urchin shaped Bi2S3/CdS hierarchical heterostructures are successfully synthesized via a convenient one-pot growth rate controlled route. The product is mainly composed of Bi2S3 nanorods and CdS nanoparticles grown on their surfaces. The formation mechanism was proposed based on the evolution of morphology as a function of solvothermal time, which involves fast formation of the initial sea-urchin shaped Bi2S3 nanoflowers, followed by the gradual growth of numerous CdS nanoparticles on Bi2S3 nanoflowers. The effects of CdS in the composites on their microstructures, optical absorption properties, and photocatalytic activity were investigated comparatively. Due to the synergetic effects between hierarchical Bi2S3 nanoflowers and CdS nanoparticles, the obtained Bi2S3/CdS hierarchical heterostructures exhibit superior catalytic activity over the independent components. Furthermore, the obtained Bi2S3/CdS hierarchical heterostructure composite shows a strong structure-induced enhancement of the photocatalytic performance for the photodegradation of rhodamine B.
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microRNA?155 promotes the proliferation of prostate cancer cells by targeting annexin 7.
Mol Med Rep
PUBLISHED: 06-26-2014
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Micro (mi)RNAs are a group of small non?coding RNA molecules that have been demonstrated to regulate the expression of genes involved in tumorigenesis. The relevance of microRNAs in the development, progression and prognosis of prostate cancer is not fully understood. miR?155 has been implicated in the induction of breast, lung and liver cancer, but its role in prostate cancer has not been investigated. In the present study, the biological function of miR?155 was investigated in prostate cancer for the first time, to the best of our knowledge. It was demonstrated that the expression of miR?155 was upregulated in prostate cancer tissues and cell lines as determined by quantitative reverse transcription?polymerase chain reaction. Furthermore, overexpression of miR?155 promoted cell proliferation, as indicated by MTT assay. Flow cytometric analysis demonstrated that inhibition of miR?155 induced cell cycle arrest and promoted apoptosis in prostate cancer cells. In addition, western blot analysis indicated that annexin (ANX)7 was significantly downregulated in prostate cancer tissues and cells. A luciferase reporter assay indicated that ANX7 was a target of miR?155, which suggested that miRNA?155 promoted the proliferation of prostate cancer cells by regulating ANX7 expression levels.
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Metastatic nasopharyngeal carcinoma outcomes in patients on Cisplatin with nolatrexed or 5-Fluorouracil.
Oncol Res Treat
PUBLISHED: 06-12-2014
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Nasopharyngeal carcinoma (NPC) is one of the most common malignant tumors in southern China. In this study, we compared the clinical efficacy and toxicity of cisplatin with nolatrexed (LP) or 5-fluorouracil (FP) for NPC.
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Adenosine receptor activation improves microcirculation in experimental intestinal ischemia/reperfusion.
Clin. Hemorheol. Microcirc.
PUBLISHED: 06-04-2014
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Gut ischemia and reperfusion (IR), e.g. in small bowel transplantation or during resuscitation, may result in severe impairment of the intestinal microcirculation. Potential sequelae are mucosal damage, loss of intestinal barrier function, bacterial translocation, systemic inflammation, multiple organ failure and death. We hypothesized a protective role for extracellular adenosine signalling in intestinal IR injury. Using intravital microscopy we investigated the effects of the adenosine receptor (AR) agonist NECA (5'-N-ethyl carboxamide adenosine) on leukocyte-endothelial interactions and capillary perfusion in the intestinal microcirculation following intestinal IR. Six groups of Lewis rats (n = 44) were studied: control, NECA (5'-N-ethyl carboxamide adenosine), IR (30 minutes of intestinal ischemia, 2 hours of reperfusion), IR + NECA, IR + NECA + MRS1754 (A2BAR antagonist), IR + NECA + DPCPX (A1AR antagonist). All substances were administered i.v. immediately after declamping of the superior mesenteric artery. Intravital microscopy was performed after 2 hours of reperfusion. Following IR we observed a significant increase of leukocyte adhesion in the intestinal submucosal venules and a reduced capillary perfusion within the muscular layers. NECA reduced leukocyte activation and improved capillary perfusion significantly. Administration of A2BAR antagonist completely reversed the NECA effect, whereas A1AR inhibition only partially abolished the action of NECA. The data support the hypothesis that adenosine signalling is involved in intestinal IR injury. A2BAR may be more important than A1AR because A2BAR inhibition by MRS1754 completely reversed the effect of the adenosine receptor agonist NECA.
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Spatially defined microsatellite analysis reveals extensive genetic mosaicism and clonal complexity in intestinal metaplastic glands.
Int. J. Cancer
PUBLISHED: 05-18-2014
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Intestinal metaplasia (IM) has been recognized as the first irreversible precancerous stage of intestinal-type gastric cancer at which genetic instabilities, such as microsatellite instability and loss of heterozygosity, can already be detected. However, the extent and clonal relationship of these genetic lesions in the precancerous tissues are not fully appreciated. In this work, we have used well established microsatellite markers to analyze the relatedness of spatially separated individual metaplastic glands as well as sub-segments within single glands from the same patients. We found that individual IM glands frequently show different marker lengths even for closely apposed IM glands, suggesting that these tissues have already gained the ability to independently evolve their genome regardless of whether or not they share a common origin. Furthermore, within individual IM glands, there is also significant intra-gland diversity in the microsatellite markers. Since most of these cells are not dividing and only have a limited lifespan, this result indicates that in each IM gland, a single dominant clone is rare, and new clones are constantly created by either progenitor cells or stem cells. This greatly enhanced ability to create de novo genetic alterations may underlie the importance of this stage in the eventual progression toward cancer. Given the widely observed phenotype switch in the early stages of many solid tumors, whether this associated genetic stability is also an intrinsic property of metaplastic transformation should be extensively characterized to further our understanding of cancer initiation. This article is protected by copyright. All rights reserved.
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Antioxidant activities and phenolic compounds of date plum persimmon ( Diospyros lotus L.) fruits.
J Food Sci Technol
PUBLISHED: 05-08-2014
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In the present study, phenolic compounds are extracted from the date plum persimmon fruits using water, methanol and acetone as solvents. Antioxidant activities of the phenolic extracts are measured using four different tests, namely, DPPH, hydroxyl radical scavenging activities, chelating and reducing power assays. All the extracts show dose dependent DPPH radical scavenging activity, reducing and chelating powers and moreover, they are well correlated with the total phenolic and total flavonoid substances, suggesting direct contribution of phenolic compounds to these activities. In further, the extracts are identified and quantified by HPLC-ECD. Results show that gallic acid is the most abundant phenolic compound, with amounts ranging between 45.49and 287.47 ?g/g dry sample. Myricetin is the dominant flavonoid in all extracts. Its level varied from 2.75 ?g/g dry sample in acetone extract to 5.28 ?g/g dry sample in water extract. On the basis of the results obtained, the date plum persimmon fruits phenolic extract is a potential source of natural antioxidants owing to its significant antioxidant activities.
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Yiqi formula enhances the antitumor effects of erlotinib for treatment of triple-negative breast cancer xenografts.
Evid Based Complement Alternat Med
PUBLISHED: 05-07-2014
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Yiqi formula (YF), a traditional herbal prescription, has long been used to treat triple-negative breast cancer (TNBC) patients. The present study aims to investigate the effects and the related mechanism of YF for treatment of TNBC xenografts. MDA-MB-231 (human TNBC) cells were subcutaneously injected into the second mammary fat pad of 40 female nude mice, which were divided into four groups: control, erlotinib (an epidermal growth factor receptor (EGFR) tyrosine kinase inhibitor), YF, and combination (YF plus erlotinib). All treatments were administered orally for 30 days. Inhibition rate of tumor weight by erlotinib, YF, and the combination was 26.47%, 17.24%, and 39.15%, respectively. Western blotting showed that YF, erlotinib, and the combination downregulated p-EGFR (P < 0.01) and p-Akt1 (pT308) (P < 0.05) and upregulated PTEN compared with control, and the combination was more efficacious than erlotinib alone (P < 0.05). Similar results were detected by immunohistochemistry. Real-time quantitative PCR showed that YF, erlotinib, and the combination increased PTEN mRNA (P < 0.05, P < 0.01) compared with control, and the combination was more efficacious than erlotinib alone (P < 0.05). In conclusion, YF can regulate the main components of the PI3K/Akt pathway in TNBC xenografts. When YF was used in combination with erlotinib, it enhanced the antitumor effects of erlotinib on TNBC xenografts. These findings suggest that YF is suitable to use for the treatment of TNBC patients.
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Accelerated partial breast irradiation with intensity-modulated radiotherapy is feasible for chinese breast cancer patients.
J Breast Cancer
PUBLISHED: 04-16-2014
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Several accelerated partial breast irradiation (APBI) techniques are being investigated in patients with early-stage breast cancer. The present study evaluated the feasibility, early toxicity, initial efficacy, and cosmetic outcomes of accelerated partial breast intensity-modulated radiotherapy (IMRT) for Chinese female patients with early-stage breast cancer after breast-conserving surgery.
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Molecular analysis of curcumin-induced polarization of murine RAW264.7 macrophages.
J. Cardiovasc. Pharmacol.
PUBLISHED: 04-09-2014
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This study aimed to investigate the effects of curcumin on macrophages polarization and possible mechanism involved, and to analyze the molecular basis of its antiatherosclerosis activity. RAW264.7 macrophages (M0) and M1 macrophages were treated with curcumin at 0, 6.25, 12.5, and 25 ?mol/L with or without GW9662. Using real-time polymerase chain reaction and Western blot analysis, we examined the phenotype markers of M1 [iNOS, interleukin (IL)-1?, IL-6, and MCP-1] and M2 (KLF4, FIZZ1, and MGL1] macrophages. Curcumin reduced the expression of the M1 phenotype markers and upregulated the expression of proliferator-activated receptor ? in M0 and M1 macrophages and IKB? in M1 macrophages. When M1 macrophages were incubated with curcumin and GW9662, the expression of the M1 phenotype markers was decreased, while IKB? was upregulated. The expression of the M2 phenotype markers in M0 and M1 macrophages was upregulated after the curcumin treatment. When M0 and M1 macrophages were incubated with curcumin and GW9662, the expression of the M2 phenotype markers was reduced. Curcumin inhibited the M1 inflammation phenotype as a result of the direct activation of IKB? and polarized the macrophages to become M2 phenotype through the activation of proliferator-activated receptor ?. These findings provide new clues to develop new drug therapy for atherosclerosis.
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Effect of deletion of cIAP2 on intestinal microcirculation in mouse endotoxemia and polybacterial sepsis.
Shock
PUBLISHED: 03-27-2014
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Deletion of the cellular inhibitor of apoptosis protein 2 (cIAP2) is capable of rendering lipopolysaccharide (LPS)-activated macrophages highly susceptible to apoptotic triggers, thereby quickly eliminating the resident macrophage population soon after the initiation of a systemic inflammatory response. The aim of our study was to evaluate the impact of cIAP2 deletion on leukocyte recruitment and capillary perfusion in experimental endotoxemia and polybacterial sepsis using intravital microscopy of the intestinal microcirculation, which is crucial in the pathogenesis of septic multiple organ failure. We studied six groups of animals: wild-type (WT) control mice, cIAP2 knockout mice, endotoxemic WT mice (5 mg/kg LPS), endotoxemic cIAP2 knockouts (5 or 50 mg/kg LPS, respectively), and WT as well as knockout mice with polybacterial sepsis (colon ascendens stent peritonitis [CASP]). Intravital microscopy of the intestinal microcirculation was performed after 1 h of endotoxemia or 12 h of CASP-induced sepsis, respectively. Intestinal microvascular blood flow was measured using laser Doppler flowmetry. After 1 h of endotoxemia (5 mg/kg LPS), we observed a significant increase of leukocyte adhesion in intestinal submucosal venules of WT mice in comparison with control animals. The cIAP2 knockout mice showed a significant reduction in leukocyte recruitment within the intestinal submucosal microvasculature after 5 or 50 mg/kg LPS challenge, respectively. Lipopolysaccharide-induced decrease in intestinal microvascular blood flow was not affected by cIAP2 inhibition. In CASP-induced sepsis, cIAP2 deletion had no effect on intestinal leukocyte recruitment. Deletion of cIAP2 resulted in reduced microvascular leukocyte recruitment within the intestinal microcirculation in endotoxemia but not in polybacterial sepsis.
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Detection of platelet microRNA expression in patients with diabetes mellitus with or without ischemic stroke.
J. Diabetes Complicat.
PUBLISHED: 03-25-2014
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The objective of this study was to investigate the role of plasma and platelet microRNAs in the occurrence of ischemic stroke in patients with diabetes mellitus.
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Glycan-functionalized fluorescent chitin nanocrystals for biorecognition applications.
Bioconjug. Chem.
PUBLISHED: 03-21-2014
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A new platform based on chitin nanocrystals has been developed for biorecognition applications. TEMPO-oxidized chitin nanocrystals (TCNs) were labeled with a fluorescent imidazoisoquinolinone dye, and simultaneously conjugated with carbohydrate ligands, resulting in dually functionalized TCNs. The biorecognition properties of the nanocrystals were probed with lectins and bacteria, resulting in selective interactions with their corresponding cognate carbohydrate-binding proteins, as visualized by optical, fluorescence, STEM, and TEM imaging. This represents a new approach to multifunctional nanomaterials based on naturally occurring polymers, holding high potential for biomedical applications.
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Elevated tropospheric ozone increased grain protein and amino acid content of a hybrid rice without manipulation by planting density.
J. Sci. Food Agric.
PUBLISHED: 03-16-2014
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Rising tropospheric ozone affects crop yield and quality. Rice protein concentration, which is closely associated with eating/cooking quality, is of critical importance to nutritional quality. The ozone effect on amino acids of rice grains was little known, especially grown under different cultivation conditions. A hybrid rice cultivar Shanyou 63 was grown in 2010 and 2011 to investigate the interactive effect of ozone exposure and planting density on rice protein quality in a free-air ozone enrichment system.
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Effect of dissolved oxygen on nitrogen and phosphorus removal and electricity production in microbial fuel cell.
Bioresour. Technol.
PUBLISHED: 03-08-2014
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Performance of a two-chamber microbial fuel cell (MFC) was evaluated with the influence of cathodic dissolved oxygen (DO). The maximum voltage, coulombic efficiency and maximum power density outputs of MFC decreased from 521 to 303 mV, 52.48% to 23.09% and 530 to 178 mW/m(2) with cathodic DO declining. Furthermore, a great deal of total phosphorus (TP) was removed owing to chemical precipitation (about 80%) and microbial absorption (around 4-17%). COD was first removed in anode chamber (>70%) then in cathode chamber (<5%). Most of nitrogen was removed when the cathodic DO was at low levels. Chemical precipitates formed in cathode chamber were verified as phosphate, carbonate and hydroxyl compound with the aid of scanning electron microscope capable of energy dispersive spectroscopy (SEM-EDS), X-ray diffractometer (XRD) and Fourier transform infrared spectroscopy (FTIR).
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Caffeic acid reduces cutaneous tumor necrosis factor alpha (TNF-?), IL-6 and IL-1? levels and ameliorates skin edema in acute and chronic model of cutaneous inflammation in mice.
Biol. Pharm. Bull.
PUBLISHED: 03-04-2014
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Caffeic acid (3,4-dihydroxycinnamic acid, CA) has been reported to have anti-inflammatory activity in animal models. However, the mechanisms underlying the anti-inflammatory effects of CA in skin inflammation are only partially understood. The present study was designed to investigate the effects and mechanisms of CA on acute and chronic skin inflammation in mice and the effect of CA in keratinocytes in vitro. The results showed that topical treatment with CA inhibited 12-O-tetradecanoyl-phorbol-13-acetate (TPA)-induced skin edema in a dose-dependent manner, leading to substantial reductions in skin thickness and tissue weight, neutrophil-mediated myeloperoxidase activity, and various histopathological indicators. The CA treatment also significantly reduced the mRNA and protein levels of tumor necrosis factor alpha (TNF-?), interleukin-6 (IL-6) and IL-1? at the application site, and the TNF-? production, the TNF-?-induced IL-6 and IL-1? production, and TNF-?-induced nuclear factor-kappa B (NF-?B) activation in human keratinocytes in vitro. Furthermore, CA was effective at reducing inflammatory damage induced by chronic TPA exposure. These results demonstrate that CA has anti-inflammatory activities in both acute and chronic contact dermatitis models via blockade of the mRNA and protein synthesis of these cytokines and neutrophil-mediated myeloperoxidase activity, and can target inflammatory mediators specifically in the keratinocytes. Taken together, the present results suggest that CA might be a therapeutic agent against inflammatory skin diseases.
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Cu(2+) ion responsive solvent-free quantum dots.
Small
PUBLISHED: 03-03-2014
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Three quantum dots (QDs) nanofluids modified with different lengths of PEG chainsare synthesized, and the property-structure relationship of QDs nanofluids is established, to achieve QDs nanofluids with tunable fluidic or optical performance. Notably, the proposed QDs nanofluids demonstrate a selective response towards Cu(2+) -based on both fluorescence and contact angle.
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Lidamycin regulates p53 expression by repressing Oct4 transcription.
Biochem. Biophys. Res. Commun.
PUBLISHED: 02-25-2014
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Antitumor antibiotic lidamycin (LDM) is widely used in the treatment of a variety of cancers. Here we demonstrated that LDM up-regulates the expression of the tumor suppressor p53 gene by repressing Oct4 transcription. We showed that low dose LDM-induced increase of p53 expression and decrease of Oct4 expression in P19 and HCT116-p53(+/+) cells. Knockdown of Oct4 expression by siRNA led to activation of p53 in both cell lines, whereas ectopical expression of Oct4 significantly inhibited p53 expression in P19 cells. LDM-induced p53 activation was blocked by ectopical expression of Oct4.
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Association Between Dopamine Beta-Hydroxylase 19-bp Insertion/Deletion Polymorphism and Major Depressive Disorder.
J. Mol. Neurosci.
PUBLISHED: 02-12-2014
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Norepinephrine and dopamine mediate important aspects of several psychoses, including major depressive disorder (MDD). Dopamine beta-hydroxylase (DBH) catalyzes the conversion of dopamine to norepinephrine in central neurons and thus is critically involved in maintaining the transformational homeostasis. Functional polymorphisms have been reported in DBH gene, including a 19-bp insertion/deletion polymorphism (DBH5'-insertion/deletion (Ins/Del)) and a single nucleotide polymorphism (-1021C/T). The purpose of this study was to investigate whether there was an association between the two functional polymorphisms and MDD in a Han Chinese population. DBH5'-Ins/Del and -1021C/T polymorphism in promoter region of DBH gene was analyzed in 313 patients with MDD and 318 healthy subjects by using polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) analysis. The results showed statistically significant associations for genotypic distribution between the DBH5'-Ins/Del polymorphism and MDD (P?=?0.007). Individuals with Del/Del genotype demonstrated a 1.72 times increased risk of MDD compared to those with insertion alleles (95 % confidence interval (CI) 1.20-2.47, P?=?0.003). Moreover, the Del/Del genotype was associated with poorer digital span and language scores than the insertion alleles in healthy subjects (P?=?0.041 and P?=?0.048, respectively). However, there was no association observed between the genotype and allele frequencies for -1021C/T and depression. Our data suggest that the DBH5'-Ins/Del polymorphism of the DBH gene may be associated with susceptibility to MDD in a Chinese population.
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Homologous recombination efficiency enhanced by inhibition of MEK and GSK3?
Genesis
PUBLISHED: 02-10-2014
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Homologous recombination in embryonic stem cells (ESCs) is widely utilized in genome engineering, particularly in the generation of gene targeted mice. However, genome engineering is often plagued by the problem of low homologous recombination efficiency. In this study, we developed a novel method to increase the efficiency of homologous recombination in ESCs by changing its culture conditions. By comparing the efficiency of different ESCs in various culture conditions, we determined that chemicals that inhibit the MEK and GSK3? pathways (2i condition) enhance homologous recombination and eliminate differences in efficiencies among cell lines. Analysis of gene expression patterns in ESCs maintained in different culture conditions has identified several homologous recombination-related candidates, including the pluripotent markers Eras and Tbx3. The results of this study suggest that homologous recombination is associated with ESC pluripotency. genesis 00:1-8, 2014. © 2014 Wiley Periodicals, Inc.
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Tumor location is a prognostic factor for survival of Chinese women with T1-2N0M0 breast cancer.
Int J Surg
PUBLISHED: 02-09-2014
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The purpose of this study was to investigate the effects of primary tumor location on the survival of Chinese women with T1-2N0M0 breast cancer.
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Allergic asthma accelerates atherosclerosis dependent on Th2 and Th17 in apolipoprotein E deficient mice.
J. Mol. Cell. Cardiol.
PUBLISHED: 01-27-2014
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The chronic inflammation of atherosclerosis is regulated by Th1, while allergic asthma is controlled by Th2. The direct relationship between atherosclerosis and asthma is contradictory. The aim of this study was to investigate the role of allergic asthma in atherosclerotic plaque formation and the change of CD4(+) T cells subsets.
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Association of BSG genetic polymorphisms with atherosclerotic cerebral infarction in the Han Chinese population.
Int. J. Neurosci.
PUBLISHED: 01-23-2014
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The Basigin (BSG, also known as CD147/extracellular matrix metalloproteinase inducer) belongs to the immunoglobulin superfamily (IgSF). It is a cellular receptor for cyclophilin A (CypA), and is originally known as tumor cell collagenase stimulatory factor (TCSF), which could abundantly expressed on the surface of tumor cells, haematopoietic, monocytes, epithelial endothelial cells and smooth muscle cells. Accumulating evidence showed that BSG played an important role in stimulating the secretion of matrix metalloproteinases (MMPs), which has been reported to be involved in the development of atherosclerosis. Since atherosclerosis is an important risk factor for atherosclerotic cerebral infarction (ACI), we speculate that BSG genetic polymorphisms may influence formation of atherosclerosis and then development of ACI. This study aimed to detect the potential association of the single nucleotide polymorphisms (SNP, -631 G > T, -318 G > C, 10141 G > A and 10826 G > A) of BSG gene in Hunan Han Chinese population with ACI. We genotyped 199 ACI patients and 188 matched healthy controls for the four BSG SNP by method of matrix-assisted laser desorption/ionization-time-offlight mass spectrometry (MALDI-TOF MS). Our results suggested that all the polymorphisms were observed in the subjects from Changsha area of Hunan Province. However, no significant difference was observed between the distribution of these SNP in cases and controls. Therefore, we speculate that BSG genetic polymorphisms might not be an important factor in the development of ACI in our Chinese Han population.
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Growth rate controlled synthesis of hierarchical Bi2S3/In2S3 core/shell microspheres with enhanced photocatalytic activity.
Sci Rep
PUBLISHED: 01-20-2014
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Core/shell heterostructure composite has great potential applications in photocatalytic field because the introduction of core can remarkably improve charge transport and enhance the electron-hole separation. Herein, hierarchical Bi2S3/In2S3 core/shell structured microspheres were prepared via a simple one-pot hydrothermal process based on different growth rate of the two kinds of sulphides. The results showed that, the as-prepared hierarchical Bi2S3/In2S3 core/shell heterostructure exhibits significant visible light photocatalytic activity for degradation of 2, 4-dichlorophenol. The introduction of Bi2S3 core can not only improve charge transport and enhance the electron-hole separation, but also broaden the visible light response. The hierarchical porous folwer-like shell of In2S3 could increase the specific surface area and remarkably enhanced the chemical stability of Bi2S3 against oxidation.
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Network dysfunction in Alzheimer's disease and frontotemporal dementia: implications for psychiatry.
Biol. Psychiatry
PUBLISHED: 01-15-2014
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Structural and functional connectivity methods are changing how researchers conceptualize and explore neuropsychiatric disease. Here, we summarize emerging evidence of large-scale network dysfunction in Alzheimer's disease and behavioral variant frontotemporal dementia, focusing on the divergent impact these disorders have on the default mode network and the salience network. We update a working model for understanding the functions of these networks within a broader anatomical context and highlight the relevance of this model for understanding psychiatric illness. Finally, we look ahead to persistent challenges in the application of network-based imaging methods to patients with Alzheimer's disease, behavioral variant frontotemporal dementia, and other neuropsychiatric conditions. Recent advances and persistent needs are discussed, with an eye toward anticipating the hurdles that must be overcome for a network-based framework to clarify the biology of psychiatric illness and aid in the drug discovery process.
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17?-estradiol promotes cholesterol efflux from vascular smooth muscle cells through a liver X receptor ?-dependent pathway.
Int. J. Mol. Med.
PUBLISHED: 01-08-2014
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Estrogen has pleiotropic effects on the cardiovascular diseases, yet the underlying mechanisms remain incompletely understood. Cholesterol efflux is a key mechanism through which to prevent foam cell formation and the development of atherosclerosis. Recent studies highlight the role of vascular smooth muscle cell (VSMC)-derived foam cells in atherogenesis. However, it remains unclear whether estrogen promotes cholesterol efflux from VSMCs and inhibits VSMC-derived foam cell formation. In the present study, we demonstrated that 17?-estradiol (E2) markedly enhanced cholesterol efflux to apolipoprotein (apo)A-1 and high-density lipoprotein (HDL) and attenuated oxidized low-density lipoprotein (ox-LDL) induced cholesteryl ester accumulation in VSMCs, which was associated with an increase in the expression of ATP-binding cassette transporters ABCA1 and ABCG1. The upregulation of ABCA1 and ABCG1 expression by E2 resulted from liver X receptor (LXR)? activation, which was confirmed by the prevention of the expression of ABCA1 and ABCG1 after inhibition of LXR? with a pharmacological inhibitor or small interfering RNA (siRNA). Furthermore, E2 increased LXR?, ABCA1 and ABCG1 expression in VSMCs via the estrogen receptor (ER), and the involvement of ER? was confirmed by the use of selective ER? or ER? antagonists (MPP and PHTPP) and agonists (PPT and DPN). These findings suggest that E2 promotes cholesterol efflux from VSMCs and reduces VSMC-derived foam cell formation via ER?- and LXR?-dependent upregulation of ABCA1 and ABCG1 and provide novel insights into the anti-atherogenic properties of estrogen.
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Size effects of Ag nanoparticles on plasmon-induced enhancement of photocatalysis of Ag-?-Fe?O? nanocomposites.
J Colloid Interface Sci
PUBLISHED: 01-07-2014
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Composite photocatalysts that consist of semiconductor and noble metal nanostructures have been considered to be the promising and crucial materials for straightforward improving the efficiency in photocatalytic process and for the conversion of solar to chemical energy. In this work, we fabricated Ag-?-Fe2O3 hybrid composites through a self-catalytic growth method by using the aldehyde-modified spindle ?-Fe2O3 nanoparticles (NPs) as supports. The size of supported Ag NPs can be directly controlled on the surface of ?-Fe2O3. The morphology and structure of the resulting Ag-?-Fe2O3 hybrid composites were studied by various techniques, including SEM, TEM, and XRD. The distinct photocatalytic behaviors were examined through the photodegradation of RhB dye. It was found that with the Ag NPs, the photocatalytic activities were enhanced greatly and the size of the Ag NPs played a crucial influence on the photocatalytic behaviors of the Ag-?-Fe2O3 composites.
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Post-mastectomy radiotherapy can improve survival in breast cancer patients aged 35 years or younger with four or more positive nodes but not in one to three positive nodes.
Ther Clin Risk Manag
PUBLISHED: 01-01-2014
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This retrospective study investigated the clinical value of post-mastectomy radiotherapy (PMRT) in female Chinese breast cancer patients aged 35 years or younger with positive axillary lymph nodes after mastectomy.
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Comparison of transverse island flap onlay and tubularized incised-plate urethroplasties for primary proximal hypospadias: a systematic review and meta-analysis.
PLoS ONE
PUBLISHED: 01-01-2014
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This meta-analysis was conducted to compare postoperative outcomes between transverse island flap (TVIF) onlay and tubularized incised-plate (TIP) urethroplasties for primary proximal hypospadias.
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The relationship between frontal lobe lesions, course of post-stroke depression, and 1-year prognosis in patients with first-ever ischemic stroke.
PLoS ONE
PUBLISHED: 01-01-2014
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Most studies on post-stroke depression (PSD) have focused on a certain time point after stroke instead of the time course of PSD. The aim of this study was to determine the relationship between frontal lobe lesions, course of PSD over a year following the stroke onset, and the 1-year prognosis in patients with first-ever ischemic stroke.
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Transdermal fentanyl for pain due to chemoradiotherapy-induced oral mucositis in nasopharyngeal cancer patients: evaluating efficacy, safety, and improvement in quality of life.
Drug Des Devel Ther
PUBLISHED: 01-01-2014
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This study evaluated the efficacy, safety, and quality of life (QoL) measure of transdermal fentanyl (TDF) for moderate-to-severe pain due to oral mucositis caused by chemoradiotherapy in patients with advanced nasopharyngeal carcinoma (NPC). Patients with NPC who experienced moderate-to-severe oral mucosal pain during chemoradiotherapy (n = 78) received TDF for pain relief. Pain relief and QoL were compared before and after treatment. The mean numeric rating scale score was reduced from 7.41 ± 0.96 before treatment to 5.54 ± 0.86, 3.27 ± 0.73, 2.88 ± 0.62, and 2.82 ± 0.68 on days 1, 4, 7, and 10, respectively, after treatment (P < 0.001). Karnofsky performance status and SPAASMS (Score for pain, Physical activity levels, Additional pain medication, Additional physician/emergency room visits, Sleep, Mood, and Side effects) scores showed significant improvement after treatment, indicating an improved QoL of patients (both P<0.001). The most common adverse reactions were nausea and vomiting (10.26%). No serious life-threatening adverse events and no symptoms of drug withdrawal were observed. TDF is effective, safe, and improves QoL in treating pain due to oral mucositis caused by chemoradiotherapy in NPC patients.
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The differentiation of human adipose-derived stem cells towards a urothelium-like phenotype in vitro and the dynamic temporal changes of related cytokines by both paracrine and autocrine signal regulation.
PLoS ONE
PUBLISHED: 01-01-2014
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To investigate the differentiation ability of human adipose-derived stem cells (ASCs) towards urothelium-like cells in vitro and the dynamic changes of related cytokines and cytokine receptors in the culture medium.
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Association of genetic variants in Wnt signaling pathway with tuberculosis in Chinese Han population.
PLoS ONE
PUBLISHED: 01-01-2014
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Compelling studies have implicated that the Wnt signaling pathway plays an important role in the development and progression of tuberculosis, however, there is little literature addressing the role of polymorphisms in Wnt pathway on tuberculosis. We took a pathway based candidate gene approach to investigate the possible correlation between genetic variants in Wnt pathway and tuberculosis. Three single nucleotide polymorphisms (SNPs) in Wnt pathway (rs4135385 in CTNNB1 gene, rs7832767 in SFRP1 gene, and rs11079571 in AXIN2 gene) were genotyped in 422 Chinese Han tuberculosis patients and 402 frequency matched (age, gender, and ethnicity) controls using high-resolution melting analysis. The genotype and allelic frequencies of rs4135385 and rs7832767 were significantly different among patients and controls. The dominant model of rs4135385 was significantly associated with an increased risk of tuberculosis (AG/GG versus AA: OR?=?1.49, 95% CI?=?1.06-2.09, p?=?0.019). The recessive model of rs7832767 posed a significant higher risk for tuberculosis (TT versus TC/CC, OR?=?2.70, 95% CI?=?1.41-5.18, p?=?0.002). These SNPs were further evaluated whether they were correlated with the site of tuberculosis and the level of inflammatory markers. Rs7832767 was significantly associated with the level of CRP (p?=?0.014), and the patients carrying T allele might present with elevated CRP values (OR?=?1.90, 95% CI?=?1.21-2.96, p?=?0.005). Our study provided the first evidence that rs4135385 and rs7832767 were associated with tuberculosis risk, and genetic variants in Wnt signaling pathway might participate in genetic susceptibility to tuberculosis in Chinese Han population. Further epidemiological and functional studies in larger populations are warranted to verify our results.
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The value of radiotherapy in breast cancer patients with isolated ipsilateral supraclavicular lymph node metastasis without distant metastases at diagnosis: a retrospective analysis of Chinese patients.
Onco Targets Ther
PUBLISHED: 01-01-2014
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The purpose of this study was to investigate the prognosis of ipsilateral supraclavicular lymph node metastasis (ISLM) without evidence of distant metastases at diagnosis in Chinese women with breast cancer and to elucidate the clinical value of adjuvant radiotherapy.
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Prognostic value of Ki-67 in breast cancer patients with positive axillary lymph nodes: a retrospective cohort study.
PLoS ONE
PUBLISHED: 01-01-2014
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Ki-67 expression is a biomarker for proliferation. Its prognostic value is recognized in breast cancer (BC) patients with negative axillary nodes, but is less clear in BC patients with positive axillary lymph nodes.
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11 nm hard X-ray focus from a large-aperture multilayer Laue lens.
Sci Rep
PUBLISHED: 11-13-2013
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The focusing performance of a multilayer Laue lens (MLL) with 43.4??m aperture, 4?nm finest zone width and 4.2?mm focal length at 12?keV was characterized with X-rays using ptychography method. The reconstructed probe shows a full-width-at-half-maximum (FWHM) peak size of 11.2?nm. The obtained X-ray wavefront shows excellent agreement with the dynamical calculations, exhibiting aberrations less than 0.3 wave period, which ensures the MLL capable of producing a diffraction-limited focus while offering a sufficient working distance. This achievement opens up opportunities of incorporating a variety of in-situ experiments into ultra high-resolution X-ray microscopy studies.
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[Effects of N-Arachidonoylethanolamine on the Quality of Platelets Stored in M-sol Platelet Preservative Solution In Vitro].
Zhongguo Shi Yan Xue Ye Xue Za Zhi
PUBLISHED: 10-26-2013
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This study was purposed to investigate the effects of N-Arachidonoylethanolamine (ANA) on the quality of platelets (Plt) stored in Plt M-sol preservative solution at 22 ± 2°C. Samples taken from collecting apheresis Plt by the Amicus instrument and splited into two equal parts were stored in Plt M-sol preservative solution on a shaker at 22 ± 2°C. Different working concentrations of ANA (from 0.1 to 50 µmol/L) were then added into one part of stored Plt as the experimental group, the other without ANA was used as the control group. The viability of Plts stored at 22 ± 2°C for 7 days was evaluated by MTT colorimetric assay. The most effective concentration of ANA was selected and added to the subsequent experimental group. Plt count (BPC), mean Plt volume (MPV), Plt distribution width (PDW), phosphatidyl serine (PS) and soluble P-selectin were detected on the 1(st), 5(th), 7(th), 9(th) and 11(th) day of storage. The results showed that the most effective working concentration of ANA was 0.5 µmol/L, which showed significant increasing Plt viability (91.23 ± 5.44%) compared to the control group (62.54 ± 4.79%). Thus, ANA concentration at 0.5 µmol/L was chosed to perform subsequent experiments. During 11 days of storage, the BPC, MPV and PDW were not changed significantly between the experimental group and control group, although there was decreasing trend in the BPC and increasing trends in MPV and PDW in the two groups. The rate of Plt PS positive was enhanced during the storage period: the rate of PS positive in experimental group increased from 7.69 ± 1.82% to 10.74 ± 1.78% while it in control group increased from 11.21 ± 2.03% to 15.37 ± 1.95%, with significant differences between the two groups (P < 0.05) on the 9(th) and 11(th) day of storage, respectively. Soluble P-selectin contents in experimental group on the 9(th) and 11(th) day of storage were 30.19 ± 2.03 ng/ml and 34.52 ± 2.64 ng/mL, respectively, while those in control group were 39.18 ± 2.66 ng/ml and 43.23 ± 2.58 ng/ml, respectively, with significant differences between the two groups (P < 0.05). It is concluded that the extended storage of Plt in M-sol treated with low concentration ANA can potentially alleviate Plt storage lesions.
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P144, A TGF-?1 Antagonist Peptide, Synergizes with Sildenafil and Enhances Erectile Response via Amelioration of Cavernosal Fibrosis in Diabetic Rats.
J Sex Med
PUBLISHED: 10-17-2013
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Patients with diabetes exhibit more severe erectile dysfunction (ED) and are less responsive to first-line oral phosphodiesterase type 5 inhibitor (PDE5i). It has been suggested that increased collagen deposition and reduced smooth muscle content in the corpus cavernosum are important mechanisms for diabetes-associated ED and that transforming growth factor-?1 (TGF-?1) is a potent fibrotic factor responsible for the structural alterations in the corpus cavernosum.
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Cognitive deficits in mild Parkinsons disease are associated with distinct areas of grey matter atrophy.
J. Neurol. Neurosurg. Psychiatr.
PUBLISHED: 10-16-2013
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The neuroanatomical substrates underlying cognitive impairment in Parkinsons disease (PD) remain poorly understood. To address this gap, we compared the grey matter atrophy patterns in PD patients with mild cognitive impairment (PD-MCI) with PD patients having no cognitive impairment (PD-NCI), and examined relationships between atrophic regions and cognitive performance in specific domains.
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[Expression and significance of interleukin-6, interferon-inducible protein-10 and interleukin-17 in serum and synovial fluid of patients with juvenile idiopathic arthritis].
Zhonghua Er Ke Za Zhi
PUBLISHED: 10-15-2013
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To detect the disparity of three cytokines interleukin-6 (IL-6), interferon-inducible protein 10 (IP-10) and interleukin-17 (IL-17) in peripheral blood (PB) and synovial fluid (SF) of patients with juvenile idiopathic arthritis (JIA).
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Lentivirus vector-mediated Rho guanine nucleotide dissociation inhibitor 2 overexpression induces beta-2 adrenergic receptor desensitization in airway smooth muscle cells.
J Thorac Dis
PUBLISHED: 09-02-2013
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Beta-2 adrenergic receptor (?2AR) downregulation is critical to asthma rescue therapy; however, tolerance, also known as ?2AR or bronchodilator desensitization, mechanisms potentially resulting in life-threatening rescue treatment failure remain poorly understood.
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Natural killer cell activity and frequency of killer cell immunoglobulin-like receptors in children with different forms of juvenile idiopathic arthritis.
Pediatr Allergy Immunol
PUBLISHED: 08-14-2013
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Juvenile idiopathic arthritis (JIA) has three major onset types with widely varying clinical features: systemic, polyarticular and pauciarticular. We assessed natural killer (NK) cell function and killer cell immunoglobulin-like receptor (KIR) genotypes in patients with different JIA subtypes.
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The role of epithelial-mesenchymal transition and IGF-1R expression in prediction of gefitinib activity as the second-line treatment for advanced nonsmall-cell lung cancer.
Cancer Invest.
PUBLISHED: 08-07-2013
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Except for EGFR gene mutation, there is still lack of predictive factors for gefitinib activity as the second-line treatments for advanced NSCLC with wild-type (WT) EGFR or patients with mutant EGFR but showed poor response. Our purpose was to assess the predictive value of epithelial-mesenchymal transition (EMT) and IGF-1R for gefitinib efficacy as the second-line treatment for NSCLC.
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[Assessing the benthic ecological status in Yangtze River Estuary using AMBI and M-AMBI].
Huan Jing Ke Xue
PUBLISHED: 08-07-2013
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Both AMBI and M-AMBI could effectively assess the benthic ecological status of estuaries and coastal systems with soft sediments, and show the response of benthic communities to human pressures and natural changes. To monitor the ecological status of Yangtze River Estuary, macroinvertebrate samples and environmental data were collected in April 2009. Results showed that the benthic habit of Yangtze River Estuary was disturbed to various degrees, especially in the watersheds of Hangzhou Bay, coastal areas of Zhoushan islands and in the inner part of Yangtze River Estuary, which was related to land resourced discharges, eutrophication and large amounts of coastal projects. No significant difference was found between the calculation results of AMBI and M-AMBI based on density and biomass, as indicated by one-way Analysis of Variance (ANOVA). Compared with AMBI, M-AMBI could be more effective to assess the ecological status of Yangtze River Estuary because M-AMBI matched the community structure and environmental variables better. Moreover, according to results of Pearson correlation and linear regression analysis, significant negative relationships were found between the eutrophication index both in the surface and bottom water layers and M-AMBI, but no significant relationship was found between the eutrophication index and AMBI. Therefore, M-AMBI could be more suitable in indicating the eutrophication stress of Yangtze River Estuary.
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Association between microRNA genetic variants and susceptibility to colorectal cancer in Chinese population.
Tumour Biol.
PUBLISHED: 08-01-2013
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MicroRNAs (miRNAs) play an important role in the pathogenesis of neoplasm. Single nucleotide polymorphisms (SNPs) within miRNAs can change their phenotype and function. We attempted to analyze the relationship between two SNP loci in miRNAs and colorectal cancer (CRC) in Chinese Han population. We genotyped the polymorphism of two common miRNA SNPs, miR-146a (rs2910164 G > C) and miR-499 (rs3746444 T > C), in a case-control study of 276 CRC cases and 373 healthy controls using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). The genotypes and allele frequencies of the two SNP loci were first compared between patients and controls and then further analyzed among subgroups of patients with different clinicopathological profiles. The rs2910164 CG genotype was significantly associated with a decreased risk of CRC [CG versus GG, odds ratio (OR)?=?0.567; 95 % confidence intervals (CIs)?=?0.338-0.952; p?=?0.031]. No significant differences of miR-499 genotype and allele distribution were detected between patients and controls. Comparison between groups divided by clinicopathologic features showed that the polymorphism of miR-146a was associated with the degree of tumor differentiation (p?=?0.014), and the G allele of rs2910164 trended to a mature differentiation (OR?=?0.553; 95 % CI?=?0.315-0.971; p?=?0.038). MiR-146a (rs2910164 G > C) polymorphism is associated with CRC susceptibility and histological differentiation in Chinese Han population.
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Zinc and myocardial ischemia/reperfusion injury.
Biometals
PUBLISHED: 07-10-2013
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As an important trace element, zinc is required for the normal cellular structure and function, and impairment of zinc homeostasis is associated with a variety of health problems including cardiovascular disease. Zinc homeostasis is regulated through zinc transporters, zinc binding molecules, and zinc sensors. Zinc also plays a critical role in cellular signaling. Studies have documented that zinc homeostasis is impaired by ischemia/reperfusion in the heart and zinc dyshomeostasis may play a role in the pathogenesis of myocardial ischemia/reperfusion injury. Both exogenous and endogenously released zinc may play an important role in cardioprotection against ischemia/reperfusion injury. The goal of this review is to summarize the current understanding of the roles of zinc homeostasis and zinc signaling in myocardial ischemia/reperfusion injury.
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Synthesis of hierarchical TiO? nanoflower with anatase-rutile heterojunction as Ag support for efficient visible-light photocatalytic activity.
Dalton Trans
PUBLISHED: 07-02-2013
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Hierarchical flower-like TiO? with an anatase-rutile heterojunction was prepared by a hydrothermal process in the presence of titanium trichloride and poly(sodium-p-styrenesulfonate) (PSS). The morphology evolution process and formation mechanism of the as-obtained products were investigated in detail. It was found that morphology and crystalline phase can be easily adjusted by changing the reaction time or solution system. On the basis of this hierarchical nanoflower structure, a visible light sensitive Ag/hierarchical flower-like TiO? plasmonic photocatalyst was fabricated. The obtained composite exhibited significantly visible-light photocatalytic activity, which could be attributed to the existence of a large number of uniformly distributed Ag-TiO? effective nanojunctions, enhanced visible light-harvesting and improved charge separation due to the migration across the anatase-rutile interface.
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Interleukin-1? -899 (+4845) C?T polymorphism increases the risk of chronic periodontitis: evidence from a meta-analysis of 23 case-control studies.
Gene
PUBLISHED: 06-21-2013
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Many epidemiological studies have indicated that interleukin-1? (IL-1?) -899 (+4845) C?T polymorphism increases the risk of chronic periodontitis (CP), whereas some studies have reported opposite results. Accordingly, the aim of this meta-analysis is to investigate the association of the IL-1? -899 (+4845) C?T polymorphism with CP. We searched the PubMed database up to May 1, 2013 and finally obtained 23 case-control studies. After data extraction, we performed meta-analysis using Comprehensive Meta-Analysis v2.2 software. The overall result based on the fixed-effect model showed that IL-1? -899 (+4845) C?T polymorphism was significantly associated with increased risk of CP: [odds ratio (OR)=1.29, 95% confidence interval (CI)=1.15-1.44, p<0.001] for T vs. C; (OR=1.59, 95%CI=1.22-2.07, p=0.0005) for TT vs. CC; (OR=1.30, 95% CI=1.12-1.51, p=0.0004) for CT vs. CC; and (OR=1.40, 95% CI=1.21-1.61, p<0.001) for (CT+TT) vs. CC; (OR=1.47, 95% CI=1.16-1.87, p=0.002) for TT vs. (CT+CC). Stratified analyses revealed that there was a significantly increased risk for Caucasians and Asians. In conclusion, current evidence showed that IL-1? -899 (+4845) C?T polymorphism probably increased the risk of CP.
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Expression of interferon-? in decidual natural killer cells from women with hypertensive disorder complicating pregnancy.
J. Obstet. Gynaecol. Res.
PUBLISHED: 06-17-2013
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Hypertensive disorder complicating pregnancy (HDCP) is one of the most frequent and serious pregnancy-related diseases, which is closely related to disorders of the maternal immune system, especially the local immune microenvironment of the maternal-fetal interface. Uterine decidual natural killer (dNK) cells are the major immune cells in the maternal-fetal interface and they play an important role in establishing and maintaining a normal pregnancy. The aim of this study was to investigate the phenotype and function of dNK cells from women with HDCP.
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A low-temperature solid-phase method to synthesize highly fluorescent carbon nitride dots with tunable emission.
Chem. Commun. (Camb.)
PUBLISHED: 06-11-2013
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Highly fluorescent graphitic carbon nitride quantum dots (g-CNQDs) with a quantum yield of 42% are synthesized by a low-temperature solid-phase method with urea and sodium citrate as the precursors. Notably, the tunable emission of g-CNQDs can be achieved by simply adjusting the molar ratio of the two reactants.
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A tri-n-octylphosphine-assisted successive ionic layer adsorption and reaction method to synthesize multilayered core-shell CdSe-ZnS quantum dots with extremely high quantum yield.
Chem. Commun. (Camb.)
PUBLISHED: 06-11-2013
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Multilayered ZnS shells are coated on a CdSe quantum dot (QD) core by a tri-n-octylphosphine-assisted successive ionic layer adsorption and reaction (TOP-SILAR) method. Notably, the quantum yield (QY) of obtained core-shell CdSe/ZnS QDs can reach up to 95% after coating with a 3-monolayered ZnS shell, and is maintained even after coating with a 6-monolayered ZnS shell, breaking through the bottleneck of low QY at high coverage of the shell.
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Ovalbumin enhances YKL-40, IL-5, GM-CSF, and eotaxin expression simultaneously in primarily cultured mouse tracheal epithelial cells.
In Vitro Cell. Dev. Biol. Anim.
PUBLISHED: 05-22-2013
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Epithelial inflammation and eosinophil infiltration are crucial for the pathogenesis of asthma. Many inflammatory mediators, such as YKL-40, interleukin -5 (IL-5), granulocyte-macrophage colony-stimulating factor (GM-CSF), and eotaxin, are important for the development of allergic airway inflammation. This study is aimed at investigating the impact of treatment with ovalbumin (OVA) on the levels of those inflammatory mediators in primarily cultured mouse tracheal epithelial cells. Mouse tracheal epithelial cells were isolated and identified by immunofluorescent staining; the isolated mouse tracheal epithelial cells expressed cytokeratins. Treatment with OVA for 24 or 48 h significantly increased the relative levels of YKL-40, IL-5, GM-CSF, and eotaxin mRNA transcripts and YKL-40, IL-5, GM-CSF, and eotaxin proteins secreted in the supernatants of cultured cells, as compared with that in the untreated control cells (P?
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Non-centrosymmetric Au-SnO2 hybrid nanostructures with strong localization of plasmonic for enhanced photocatalysis application.
Nanoscale
PUBLISHED: 05-21-2013
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We present an innovative approach to the production of sub-100 nm hollow Au-SnO2 hybrid nanospheres, employing a low-cost, surfactant-free and environmentally friendly solution-based route. The hollow hybrid nanostructures were synthesized using a seed-mediated hydrothermal method, which can be divided into two stages: (1) formation of multicore-shell Au@SnO2 nanoparticles (NPs) and (2) thermal diffusion and ripening to form hollow Au-SnO2 hybrid NPs. The morphology, optical properties and formation mechanism were determined by a collection of joint techniques. Photocatalytic degradation of Rhodamine B (RhB) in the liquid phase served as a probe reaction to evaluate the activity of the as-prepared hollow hybrid Au-SnO2 NPs under the irradiation of both visible light and ultraviolet light. Significantly, the as-obtained Au-SnO2 hybrid nanostructures exhibited enhanced visible light or UV photocatalytic abilities, remarkably superior to commercial pure SnO2 products and P25 TiO2, mainly owing to the effective electron hole separation at the SnO2-Au interfaces and strong localization of plasmonic near-fields effects.
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Serum levels of CEA and CA15-3 in different molecular subtypes and prognostic value in Chinese breast cancer.
Breast
PUBLISHED: 05-14-2013
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The prognostic significance of preoperative carcinoembryonic antigen (CEA) and cancer antigen 15-3 (CA15-3) levels in breast cancer is controversial. This study evaluated the prognostic value of preoperative serum CEA and CA15-3 levels in Chinese breast cancer patients. A total of 470 patients with breast cancer had preoperative CEA and CA15-3 concentrations measured. The relationships between preoperative concentration and clinicopathological factors and outcomes were determined. CEA and CA15-3 levels were increased in 34 (7.2%) and 58 (12.3%) patients, respectively. Elevations of serum CEA and CA-15-3 levels correlated with the primary tumor size and axillary lymph node status. CEA levels were lower in patients with triple-negative breast cancer than in those with other subtypes (P = 0.002). The 5-year distant metastasis-free survival (DMFS), disease-free survival (DFS), and overall survival (OS) of CEA-negative vs. CEA-positive patients were 84.1% vs. 54.5% (P < 0.001), 82.7% vs. 54.8% (P < 0.001), and 89.7% vs. 78.5% (P = 0.007), respectively. The 5-year DMFS, DFS, and OS of CA15-3-negative vs. CA15-3-positive patients were 84.0% vs. 69.6% (P = 0.002), 83.0% vs. 66.2% (P < 0.001), 90.9% vs. 74.2% (P = 0.005), respectively. Multivariate analysis of prognosis indicated that CEA and CA15-3 levels were independent prognostic factors for DMFS (P = 0.021) and DFS (P = 0.032), and DFS (P = 0.014) and OS (P = 0.032), respectively. Serum levels of CEA and CA15-3 may differ in breast cancer molecular subtypes and preoperative levels of CEA and CA15-3 have a significant effect on prognosis in Chinese women with breast cancer.
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Controllable synthesis of recyclable core-shell ?-Fe2O3@SnO2 hollow nanoparticles with enhanced photocatalytic and gas sensing properties.
Phys Chem Chem Phys
PUBLISHED: 04-23-2013
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Composite materials containing different components with well-defined structures may cooperatively enhance their performance and extend their applications. In this work, core-shell ?-Fe2O3@SnO2 hollow nanoparticles (NPs) were synthesized by a low-cost and environmentally friendly seed-mediated hydrothermal method. Firstly, the ?-Fe2O3 hollow NPs were synthesized by a template-free method. Then they were used as the cores for the growth of SnO2 shells. The thickness of the shell can be simply tailored by controlling the reaction time. Various techniques, including SEM, XRD, TEM and HRTEM, were employed to investigate the morphology, structure and formation process of the special core-shell hollow structures. The combination of magnetic semiconductor (?-Fe2O3) and wide band-gap semiconductor (SnO2) endowed them with great potential to be used as recyclable photocatalysts. Experiments on photo-degradation of Rhodamin B (RhB) dye in the presence of the samples showed that the hybrid structures possessed higher photocatalytic activities than the monomer structures of SnO2 and ?-Fe2O3 materials indicating a strong coupling enhancement effect between the wide and narrow band-gap semiconductors. Moreover, the gas sensing tests of the ?-Fe2O3@SnO2 hollow NPs revealed that the samples exhibited fast response and recovery rates, which enable them to be promising materials for gas sensors.
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Factors of Hamilton Depression Rating Scale (17 items) at 2 weeks correlated with poor outcome at 1 year in patients with ischemic stroke.
Neurol. Sci.
PUBLISHED: 04-02-2013
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There was fewer paper about the relation between the Hamilton Depression Rating Scale (17 Items, HDRS-17) factors and stroke outcomes. Our aim was to investigate the influence of total score and factors of HDRS-17 on outcome of ischemic stroke at 1 year. A total of 1,953 patients with acute ischemic stroke were enrolled into a multicentered and prospective cohort study. The HDRS-17 was used to assess symptoms at 2 weeks after ischemic stroke. The Modified Ranking Scale (mRS) scores of 3-6 points and 0-2 points were regarded as poor outcome and benign outcome, respectively. At 1 year, 1,753 (89.8 %) patients had mRS score data. After adjusting for the confounders, patients with a total HDRS-17 score of ?8 had a worse outcome at 1 year (OR = 1.62, 95 % CI 1.18-2.23). Symptoms of suicide (OR = 1.89, 95 % CI 1.27-2.83), decreased or loss of interest of work (OR = 1.89, 95 % CI 1.38-2.58), retardation (OR = 1.74, 95 % CI 1.27-2.38), psychic anxiety (OR = 1.72, 95 % CI 1.26-2.34), and agitation (OR = 1.61, 95 % CI 1.08-2.40) increased the risks for poor outcome by >60 %, respectively. Depressed mood, somatic anxiety, somatic symptoms-gastrointestinal, and early insomnia also increased the risk for poor outcome by nearly 50 %, respectively. A total HDRS-17 score of ?8, and suicide, decreased or loss of interest of work, anxiety, agitation, retardation, depressed mood, somatic anxiety, somatic symptoms-gastrointestinal, and early insomnia of the HDRS-17 factors at 2 weeks after ischemic stroke increase the risk for poor outcome at 1 year.
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Intrinsic connectivity network disruption in progressive supranuclear palsy.
Ann. Neurol.
PUBLISHED: 03-27-2013
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Progressive supranuclear palsy (PSP) has been conceptualized as a large-scale network disruption, but the specific network targeted has not been fully characterized. We sought to delineate the affected network in patients with clinical PSP.
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ZrO2-functionalized magnetic mesoporous SiO2 as effective phosphate adsorbent.
J Colloid Interface Sci
PUBLISHED: 03-25-2013
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Phosphate pollution may cause eutrophication of the aquatic environment. In the present study, magnetic mesoporous SiO2 (denoted as MMS) and ZrO2-functionalized magnetic mesoporous SiO2 (denoted as ZrO2-MMS) were prepared and phosphate adsorption over the materials was investigated. The adsorbents were characterized by X-ray diffraction, transition electron microscopy, vibration sample magnetometer, N2 adsorption/desorption, zeta-potential measurement, and X-ray photoelectron spectroscopy. The results showed that MMS consisted of magnetite with particle sizes of 10-20 nm and ordered mesoporous SiO2 with the most probable pore diameter of 2.0 nm. The adsorbents could be readily separated and recovered under external magnetic field. The surface grafting of ZrO2 onto MMS led to an increase in surface zeta potential due to the formation of covalently linked ZrO2 functionality on the surface of MMS. Moreover, ZrO2 functionalization resulted in enhanced phosphate adsorption. Phosphate adsorption isotherms over the adsorbents could be well described by the Freundlich model. Phosphate adsorption kinetics followed the pseudo-second-order kinetics and the adsorption rate decreased with initial phosphate concentration. Additionally, increasing pH led to suppressed phosphate adsorption, and phosphate adsorption slightly increased with ionic strength.
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Rapid analysis of mono-saccharides and oligo-saccharides in hydrolysates of lignocellulosic biomass by HPLC.
Biotechnol. Lett.
PUBLISHED: 03-25-2013
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HPLC using pre-column derivatization with 1-phenyl-3-methyl-5-pyrazolone (PMP) was used to analyse mono-saccharides and oligo-saccharides in hydrolysates of lignocellulosic biomass. PMP derivatives, including those of mannose, rhamnose, cellobiose, glucose, xylose and arabinose, were separated within 14 min with detection at 254 nm. The method was also suitable for xylo-oligosaccharides (XOS): PMP derivatives of xylohexaose, xylopentaose, xylotetraose, xylotriose and xylobiose were well separated under the same conditions. The method was used to determine the mono-saccharide composition of Miscanthus and evaluate the production of XOS from enzymatic hydrolysis of crude xylan.
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Association between SNPs in microRNA-machinery genes and tuberculosis susceptibility in Chinese Tibetan population.
Mol. Biol. Rep.
PUBLISHED: 03-12-2013
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Tuberculosis (TB) is caused by infection with Mycobacterium tuberculosis and remains a leading cause of morbidity and mortality caused by infectious agents worldwide. Although our current understanding of the pathogenesis of TB is far from clear, there is a growing body of evidence suggesting a genetic contribution to the etiology of TB. By analyzing 294 TB cases and 287 healthy controls in a Chinese Tibetan population, we used a candidate gene approach to evaluate the association between six single nucleotide polymorphisms (rs10719, rs3757, rs3742330, rs636832, rs7813, and rs3744741) in microRNA machinery genes and TB susceptibility. The genotypic distributions of rs3757 and rs3744741 in controls were not in accordance with the Hardy–Weinberg Equilibrium (P < 0.05). Logistic regression analysis demonstrated that subjects carrying rs3742330 GG genotype had significantly decreased risk for TB than individuals carrying AA genotype [odds ratio (OR) = 0.31, 95 % confidence interval (CI) 0.12–0.75, P = 0.004. Carrying the G allele of rs3742330 was associated with a 27 % decreased risk for TB (95 % CI 0.55–0.97, P = 0.03). However, no significant associations were found for rs10719, rs636832 and rs7813. Computational modeling suggests that the rs3742330 lies within a predicted binding site (seed region) for microRNA-632 (miR-632) and that the G allele alters the affinity of microRNA-mRNA binding by disrupting the local structure of dicer 1, ribonuclease type III (DICER) mRNA, presumably allowing for upregulated DICER expression. Taken together, our data suggest that common genetic variations DICER may influence TB risk, possibly through miR-632-mediated regulation. Replication of our studies in other populations will strengthen our understanding of this association.
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JoVE Visualize is a tool created to match the last 5 years of PubMed publications to methods in JoVE's video library.

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