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Find video protocols related to scientific articles indexed in Pubmed.
[Value of dual-source computed tomography angiography of coronary artery for preoperative evaluation of old patients scheduled for noncardiac surgery].
Zhongguo Yi Xue Ke Xue Yuan Xue Bao
PUBLISHED: 09-02-2014
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To assess the role of computed tomography angiography (CTA) using dual-source computed tomography in the preoperative evaluation of old patients scheduled for noncardiac surgery.
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Structure of the gas vesicle protein GvpF from the cyanobacterium Microcystis aeruginosa.
Acta Crystallogr. D Biol. Crystallogr.
PUBLISHED: 07-27-2014
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Gas vesicles are gas-filled proteinaceous organelles that provide buoyancy for bacteria and archaea. A gene cluster that is highly conserved in various species encodes about 8-14 proteins (Gvp proteins) that are involved in the formation of gas vesicles. Here, the first crystal structure of the gas vesicle protein GvpF from Microcystis aeruginosa PCC 7806 is reported at 2.7?Å resolution. GvpF is composed of two structurally distinct domains (the N-domain and C-domain), both of which display an ?+? class overall structure. The N-domain adopts a novel fold, whereas the C-domain has a modified ferredoxin fold with an apparent variation owing to an extension region consisting of three sequential helices. The two domains pack against each other via interactions with a C-terminal tail that is conserved among cyanobacteria. Taken together, it is concluded that the overall architecture of GvpF presents a novel fold. Moreover, it is shown that GvpF is most likely to be a structural protein that is localized at the gas-facing surface of the gas vesicle by immunoblotting and immunogold labelling-based tomography.
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[Chronic natural killer cell lymphocytosis: eight cases report and literature review].
Zhonghua Xue Ye Xue Za Zhi
PUBLISHED: 07-24-2014
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To identify the characteristics of chronic natural killer cell lymphocytosis (CNKL).
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Zeolitic Imidazolate Frameworks: Next-Generation Materials for Energy-Efficient Gas Separations.
ChemSusChem
PUBLISHED: 07-08-2014
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Industrial separation processes comprise approximately 10?% of the global energy demand, driven largely by the utilization of thermal separation methods (e.g., distillation). Significant energy and cost savings can be realized using advanced separation techniques such as membranes and sorbents. One of the major barriers to acceptances of these techniques remains creating materials that are efficient and productive in the presence of aggressive industrial feeds. One promising class of emerging materials is zeolitic imidazolate frameworks (ZIFs), an important thermally and chemically stable subclass of metal organic frameworks (MOFs). The objectives of this paper are (i)?to provide a current understanding of the synthetic methods that enable the immense tunability of ZIFs, (ii)?to identify areas of success and areas for improvement when ZIFs are used as adsorbents, (iii)?to identify areas of success and areas for improvement in ZIF membranes. A review is given of the state-of-the-art in ZIF synthesis procedures and novel ZIF formation pathways as well as their application in energy efficient separations.
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A quasi-optical vector near-field measurement system at terahertz band.
Rev Sci Instrum
PUBLISHED: 07-03-2014
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This paper describes a vector near-field measurement system at terahertz band based on a high sensitivity superconducting receiver equipped with a quasi-optical probe for high resolution near-field sensing. A novel single-receiver rather than commonly used dual-receiver configuration is adopted for vector measurement. Performances of the measurement system including stability and dynamic range are studied. Vector near-field measurement of a diagonal feedhorn at 850 GHz is presented and shows good agreement with simulation and direct far-field measurement.
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[Identification and characterization of clinical features and gene mutation in a patient with iron refractory iron deficiency anemia (IRIDA)].
Zhonghua Xue Ye Xue Za Zhi
PUBLISHED: 07-03-2014
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To report the clinical data of a case of iron-refractory iron deficiency anemia (IRIDA), so as to improve the understanding of IRIDA.
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Thermoregulatory behavior is widespread in the embryos of reptiles and birds.
Am. Nat.
PUBLISHED: 02-05-2014
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Recent studies have demonstrated that thermoregulatory behavior occurs not only in posthatching turtles but also in turtles prior to hatching. Does thermoregulatory behavior also occur in the embryos of other reptile and bird species? Our experiments show that such behavior is widespread but not universal in reptile and bird embryos. We recorded repositioning within the egg, in response to thermal gradients, in the embryos of three species of snakes (Xenochrophis piscator, Elaphe bimaculata, and Zaocys dhumnades), two turtles (Chelydra serpentina and Ocadia sinensis), one crocodile (Alligator sinensis), and four birds (Coturnix coturnix, Gallus gallus domesticus, Columba livia domestica, and Anas platyrhynchos domestica). However, we detected no significant thermoregulation by the embryos of two lizard species (Takydromus septentrionalis and Phrynocephalus frontalis). Overall, embryonic thermoregulatory behavior is widespread in reptile as well as bird species but may be unimportant in the small eggs laid by most lizards.
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Crystal structures and catalytic mechanism of the C-methyltransferase Coq5 provide insights into a key step of the yeast coenzyme Q synthesis pathway.
Acta Crystallogr. D Biol. Crystallogr.
PUBLISHED: 01-23-2014
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Saccharomyces cerevisiae Coq5 is an S-adenosyl methionine (SAM)-dependent methyltransferase (SAM-MTase) that catalyzes the only C-methylation step in the coenzyme Q (CoQ) biosynthesis pathway, in which 2-methoxy-6-polyprenyl-1,4-benzoquinone (DDMQH2) is converted to 2-methoxy-5-methyl-6-polyprenyl-1,4-benzoquinone (DMQH2). Crystal structures of Coq5 were determined in the apo form (Coq5-apo) at 2.2?Å resolution and in the SAM-bound form (Coq5-SAM) at 2.4?Å resolution, representing the first pair of structures for the yeast CoQ biosynthetic enzymes. Coq5 displays a typical class I SAM-MTase structure with two minor variations beyond the core domain, both of which are considered to participate in dimerization and/or substrate recognition. Slight conformational changes at the active-site pocket were observed upon binding of SAM. Structure-based computational simulation using an analogue of DDMQH2 enabled us to identify the binding pocket and entrance tunnel of the substrate. Multiple-sequence alignment showed that the residues contributing to the dimeric interface and the SAM- and DDMQH2-binding sites are highly conserved in Coq5 and homologues from diverse species. A putative catalytic mechanism of Coq5 was proposed in which Arg201 acts as a general base to initiate catalysis with the help of a water molecule.
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Coexistence of cages and one-dimensional channels in a porous MOF with high H(2) and CH(4) uptakes.
Chem. Commun. (Camb.)
PUBLISHED: 01-10-2014
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A porous metal-organic framework with high surface area was designedly synthesized, in which polyhedral cages and one-dimensional channels coexist. It shows a total gravimetric H2 uptake of 11.35 wt% at 77 K and 90 bar and a total CH4 uptake of 305.07 cm(3) g(-1) at 298 K and 35 bar.
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Cell-cell adhesions and cell contractility are upregulated upon desmosome disruption.
PLoS ONE
PUBLISHED: 01-01-2014
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Desmosomes are perturbed in a number of disease states - including genetic disorders, autoimmune and bacterial diseases. Here, we report unexpected changes in other cell-cell adhesion structures upon loss of desmosome function. We found that perturbation of desmosomes by either loss of the core desmosomal protein desmoplakin or treatment with pathogenic anti-desmoglein 3 (Dsg3) antibodies resulted in changes in adherens junctions consistent with increased tension. The total amount of myosin IIA was increased in desmoplakin-null epidermis, and myosin IIA became highly localized to cell contacts in both desmoplakin-null and anti-Dsg3-treated mouse keratinocytes. Inhibition of myosin II activity reversed the changes to adherens junctions seen upon desmosome disruption. The increased cortical myosin IIA promoted epithelial sheet fragility, as myosin IIA-null cells were less susceptible to disruption by anti-Dsg3 antibodies. In addition to the changes in adherens junctions, we found a significant increase in the expression of a number of claudin genes, which encode for transmembrane components of the tight junction that provide barrier function. These data demonstrate that desmosome disruption results in extensive transcriptional and posttranslational changes that alter the activity of other cell adhesion structures.
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Construction of Two Microporous Metal-Organic Frameworks with flu and pyr Topologies Based on Zn4(?3-OH)2(CO2)6 and Zn6(?6-O)(CO2)6 Secondary Building Units.
Inorg Chem
PUBLISHED: 12-30-2013
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By employment of a tripodal phosphoric carboxylate ligand, tris(4-carboxylphenyl)phosphine oxide (H3TPO), two novel porous metal-organic frameworks, namely, [Zn4(?3-OH)2(TPO)2(H2O)2] (1) and [Zn6(?6-O)(TPO)2](NO3)4·3H2O (2), have been synthesized by solvothermal methods. Complexes 1 and 2 exhibit three-dimensional microporous frameworks with flu and pyr topologies and possess rare butterfly-shaped Zn4(?3-OH)2(CO2)6 and octahedral Zn6(?6-O)(CO2)6 secondary building units, respectively. Large cavities and one-dimensional channels are observed in these two frameworks. Gas-sorption measurements indicate that complex 2 has a good H2 uptake capacity of 171.9 cm(3) g(-1) (1.53 wt %) at 77 K and 1.08 bar, and its ideal adsorbed solution theory calculation predicts highly selective adsorption of CO2 over N2 and CH4. Furthermore, complexes 1 and 2 exhibit excellent blue emission at room temperature.
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Pleuropneumonectomy for diffuse pleural metastasis in primary lung cancer.
J Cancer Res Ther
PUBLISHED: 10-19-2013
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The purpose of this study is to analyze a single institution experience with pleuropneumonectomy for pleural metastasis and malignant pleural effusion in primary lung cancer.
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Structural and biochemical analyses of Microcystis aeruginosa O-acetylserine sulfhydrylases reveal a negative feedback regulation of cysteine biosynthesis.
Biochim. Biophys. Acta
PUBLISHED: 09-16-2013
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O-acetylserine sulfhydrylase (OASS) catalyzes the final step of cysteine biosynthesis from O-acetylserine (OAS) and inorganic sulfide in plants and bacteria. Bioinformatics analyses combined with activity assays enabled us to annotate the two putative genes of Microcystis aeruginosa PCC 7806 to CysK1 and CysK2, which encode the two 75% sequence-identical OASS paralogs. Moreover, we solved the crystal structures of CysK1 at 2.30? and cystine-complexed CysK2 at 1.91?, revealing a quite similar overall structure that belongs to the family of fold-type II PLP-dependent enzymes. Structural comparison indicated a significant induced fit upon binding to the cystine, which occupies the binding site for the substrate OAS and blocks the product release tunnel. Subsequent enzymatic assays further confirmed that cystine is a competitive inhibitor of the substrate OAS. Moreover, multiple-sequence alignment revealed that the cystine-binding residues are highly conserved in all OASS proteins, suggesting that this auto-inhibition of cystine might be a universal mechanism of cysteine biosynthesis pathway.
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Actin-related protein2/3 complex regulates tight junctions and terminal differentiation to promote epidermal barrier formation.
Proc. Natl. Acad. Sci. U.S.A.
PUBLISHED: 09-16-2013
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The epidermis provides an essential seal from the external environment and retains fluids within the body. To form an effective barrier, cells in the epidermis must form tight junctions and terminally differentiate into cornified envelopes. Here, we demonstrate that the branched actin nucleator, the actin-related protein (Arp)2/3 complex, is unexpectedly required for both these activities. Loss of the ArpC3 subunit of the Arp2/3 complex resulted in minimal changes in the morphogenesis and architecture of this stratified squamous epithelium, but resulted in profound defects in its physiology. Mutant embryos did not develop an effective barrier to the external environment and died within hours of birth. We discovered two underlying causes for these effects. First, ArpC3 was essential for robust assembly and function of tight junctions, specialized cell-cell adhesions that restrict water loss in the epidermis. Second, there were defects in differentiation of the epidermis and the production of cornified envelopes, structures essential for barrier activity. Underlying this defect, we found that YAP was inappropriately active not only in the ArpC3 mutant tissue, but also in cultured cells. Inhibition of YAP activity rescued the differentiation and barrier defects caused by loss of ArpC3. These results demonstrate previously unappreciated roles for the Arp2/3 complex and highlight the functions of branched actin networks in a complex tissue.
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[Predicting early response to immunosuppressive therapy in severe aplastic anemia by soluble transferrin receptor assay].
Zhonghua Xue Ye Xue Za Zhi
PUBLISHED: 08-28-2013
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To evaluate the value of serum soluble transferrin receptor (sTfR) concentration in predicting early response to immunosuppressive therapy (IST) in severe aplastic anemia (SAA).
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[Pure red cell aplasia with thymoma/T-cell large granular lymphocyte leukemia: two cases report and literatures review].
Zhonghua Xue Ye Xue Za Zhi
PUBLISHED: 07-06-2013
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To investigate the clinical and laboratory features of 2 cases of pure red cell aplasia (PRCA) with thymoma/T-cell large granular lymphocyte leukemia (T-LGLL), and to improve the recognition of the disease and the role of lymphocyte in its mechanism.
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[High-dose cyclophosphamide for severe aplastic anemia associated with ?-thalassemia: a case report and literatures review].
Zhonghua Xue Ye Xue Za Zhi
PUBLISHED: 07-06-2013
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To investigate the clinical features and therapeutic method for severe aplastic anemia (SAA) associated with ?-thalassemia, and to improve the recognition of the disease.
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Structure and catalytic mechanism of yeast 4-amino-4-deoxychorismate lyase.
J. Biol. Chem.
PUBLISHED: 07-01-2013
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Saccharomyces cerevisiae Abz2 is a pyridoxal 5-phosphate (PLP)-dependent lyase that converts 4-amino-4-deoxychorismate (ADC) to para-aminobenzoate and pyruvate. To investigate the catalytic mechanism, we determined the 1.9 ? resolution crystal structure of Abz2 complexed with PLP, representing the first eukaryotic ADC lyase structure. Unlike Escherichia coli ADC lyase, whose dimerization is critical to the formation of the active site, the overall structure of Abz2 displays as a monomer of two domains. At the interdomain cleft, a molecule of cofactor PLP forms a Schiff base with residue Lys-251. Computational simulations defined a basic clamp to orientate the substrate ADC in a proper pose, which was validated by site-directed mutageneses combined with enzymatic activity assays. Altogether, we propose a putative catalytic mechanism of a unique class of monomeric ADC lyases led by yeast Abz2.
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A series of novel zinc(II) entangled coordination polymers based on carboxyphenyl-terpyridine ligands.
Dalton Trans
PUBLISHED: 05-23-2013
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Hydrothermal synthesis has afforded five divalent zinc coordination polymers containing 4-(4-carboxyphenyl)-2,2:6,2-terpyridine (HL1) or its isomer 4-(4-carboxyphenyl)-2,2:4,4-terpyridine (HL2), with or without the addition of auxiliary ligands, 1,3,5-benzenetricarboxylic acid (H3btc) and 1,4-benzenedicarboxylic acid (H2bdc). Their structures have been characterized by single crystal X-ray analyses and further characterized by infrared spectra, elemental analyses, powder X-ray diffraction, thermogravimetric analyses and photoluminescent spectra. Across this series, the ?···? interactions have a dramatic impact on the self-assembly of these entanglement structures, in either case it can exert an important structure-directing role. In addition, the disposition of pyridine nitrogen atoms in ligands also plays a large role in structure direction in this system. Complex 1 is a 2D + 2D?3D inclined polycatenated coordination polymer based on the resulting array of 2D (6,3) layers constructed by 1D?2D ?···? directed self-assembly. Complex 2 is assembled into a 3D framework by means of 1D + 1D?3D mutual interdigitation based on 1D?1D self-assembly driven by ?···? stacking interactions. Complex 3 shows a 2D + 2D?3D interdigital network involving 2D + 2D?2D parallel interpenetrated and 2D + 2D?2D interdigital (4,4) layer motifs. Complex 4 displays a 2D + 2D?3D polythreaded framework based on a 2D (4,4) network comprised of alternating rings and rods. Complex 5 is a (3,4)-connected 3D framework with topology (4.8(2).10(3))(4.8(2)). In comparison with covalently connected entanglements, such ?···? directing self-assembly of entanglements are far less explored, especially, polycatenane based on 1D chain motifs and polythread based on 2D layer motifs are rarely reported. Furthermore, the luminescent properties of complexes 1-5 at room temperature have also been studied in detail herein.
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A safety study of inactivated Enterovirus 71 vaccine.
Hum Vaccin Immunother
PUBLISHED: 04-09-2013
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Enterovirus 71 (EV71) is one of the major causative agents for hand, foot and mouth disease (HFMD) in childhood. Nowadays, HFMD or EV71 infections have already become an important public health issue throughout the world. Vaccination may be the most effective measure to control the transmission of the virus. Therefore, to pave EV71 vaccine into human clinical trial, in the present study a comprehensive preclinical safety assessment of inactivated EV71 vaccine including single- and repeat-dose toxicity studies were conducted in rats and cynomolgus monkeys. No abnormal findings were observed in rats following single intramuscular administration with EV71 vaccine (640 U). The results also showed no obvious systemic toxicities from four repetitive intramuscular injections, with a 14-d interval, of two dosages of EV71 vaccine in the two animal species. Antinuclear antibody response was not detected after the repeated administrations. Histopathological examination demonstrated the minimal to severe inflammatory changes in muscle tissues of the injection sites in EV71 vaccine-injected animals and most of findings have been improved over time. Furthermore, test article could induce highly EV71-specfic neutralizing antibody response in both animal species. Taken together, these data suggested a favorable safety profile for inactivated EV71 vaccine and supported this product to enter human phase I clinical trial.
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[Detection of anaerobes and drug sensitivity from the periodontal pockets of patients with combined periodontal-endodontic lesions].
Shanghai Kou Qiang Yi Xue
PUBLISHED: 04-05-2013
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To investigate the correlation between the composition of bacterial flora isolated from deep periodontal pockets with combined periodontal-endodontic lesions, and to test the antibiotic susceptibility of anaerobes.
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Heterologous expression and characterization of bacterial 2-C-methyl-D-erythritol-4-phosphate pathway in Saccharomyces cerevisiae.
Appl. Microbiol. Biotechnol.
PUBLISHED: 03-22-2013
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Transfer of a biosynthetic pathway between evolutionary distant organisms can create a metabolic shunt capable of bypassing the native regulation of the host organism, hereby improving the production of secondary metabolite precursor molecules for important natural products. Here, we report the engineering of Escherichia coli genes encoding the 2-C-methyl-D-erythritol-4-phosphate (MEP) pathway into the genome of Saccharomyces cerevisiae and the characterization of intermediate metabolites synthesized by the MEP pathway in yeast. Our UPLC-MS analysis of the MEP pathway metabolites from engineered yeast showed that the pathway is active until the synthesis of 2-C-methyl-D-erythritol-2,4-cyclodiphosphate, but appears to lack functionality of the last two steps of the MEP pathway, catalyzed by the [4Fe-4S] iron sulfur cluster proteins encoded by ispG and ispH. In order to functionalize the last two steps of the MEP pathway, we co-expressed the genes for the E. coli iron sulfur cluster (ISC) assembly machinery. By deleting ERG13, thereby incapacitating the mevalonate pathway, in conjunction with labeling experiments with U-¹³C? glucose and growth experiments, we found that the ISC assembly machinery was unable to functionalize ispG and ispH. However, we have found that leuC and leuD, encoding the heterodimeric iron-sulfur cluster protein, isopropylmalate isomerase, can complement the S. cerevisiae leu1 auxotrophy. To our knowledge, this is the first time a bacterial iron-sulfur cluster protein has been functionally expressed in the cytosol of S. cerevisiae under aerobic conditions and shows that S. cerevisiae has the capability to functionally express at least some bacterial iron-sulfur cluster proteins in its cytosol.
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Mesenchymal stem cells induce granulocytic differentiation of acute promyelocytic leukemic cells via IL-6 and MEK/ERK pathways.
Stem Cells Dev.
PUBLISHED: 03-15-2013
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All-trans retinoic acid (ATRA) induces clinical remission in most acute promyelocytic leukemia (APL) patients by inducing terminal differentiation of APL cells toward mature granulocytes. Here we report that human umbilical cord-derived mesenchymal stem cells (UC-MSCs) are capable of inducing granulocytic differentiation of the APL-derived NB4 cell line as well as primary APL cells and also cooperate with ATRA in an additive manner. Transwell coculture experiments revealed that UC-MSCs differentiation-inducing effect was mediated through some soluble factors. Differentiation attenuation by IL-6Ra neutralization and induction by addition of exogenous IL-6 confirmed that IL-6 secreted by UC-MSCs was at least partially responsible for this differentiation induction process. Moreover, we found that UC-MSCs activated the MEK/ERK signaling pathway in promyelocytic cells and pharmacological inhibition of the MEK/ERK pathway reversed UC-MSC-induced differentiation, indicating that UC-MSCs exerted effect through activation of the MEK/ERK signaling pathway. These results demonstrate for the first time a stimulatory effect of MSCs on the differentiation of APL cells and bring a new insight into the interaction between MSCs and leukemic cells. Our data suggest that UC-MSCs/ATRA combination could be used as a novel therapeutic strategy for APL patients.
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Biocompatibility assessment of a long-term wearable artificial pump-lung in sheep.
Artif Organs
PUBLISHED: 03-03-2013
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The purpose of this study was to assess the biocompatibility of a newly developed long-term wearable artificial pump-lung (APL) in a clinically relevant ovine animal model. The wearable APL device was implanted in five sheep through left thoracotomy. The device was connected between the right atrium and pulmonary artery and evaluated for 30 days. Three sheep were used as the sham control. Platelet activation was assessed by measuring platelet surface P-selectin (CD62P) expression with flow cytometry and plasma soluble P-selectin with an enzyme-linked immunosorbent assay. Thrombotic deposition on the device components and hollow fiber membranes were analyzed with digital imaging and scanning electron microscopy. Surface P-selectin of the APL and sham groups changed significantly over the study period, but without significant differences between the two groups. Soluble P-selectin for the two groups peaked in the first 24?h after the surgery. Soluble P-selectin of the APL group remained slightly elevated over the study period compared to the presurgical baseline value and was slightly higher compared to that of the sham group. Plasma free hemoglobin remained in the normal ranges in all the animals. In spite of the surgery-related alteration in laboratory tests and elevation of platelet activation status, the APL devices in all the animals functioned normally (oxygen transfer and blood pumping) during the 30-day study period. The device flow path and membrane surface were free of gross thrombus. Electron microscopy images showed only scattered thrombi on the fibers (membrane surface and weft). In summary, the APL exhibited excellent biocompatibility. Two forms of platelet activation, surgery-related and device-induced, in the animals implanted with the wearable APL were observed. The limited device-induced platelet activation did not cause gross thrombosis and impair the long-term device performance.
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Optimization of amorphadiene synthesis in bacillus subtilis via transcriptional, translational, and media modulation.
Biotechnol. Bioeng.
PUBLISHED: 02-22-2013
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Genetically engineered microbes have been intensively investigated as a means for the cost-effective production of isoprenoids. Bacillus subtilis is a promising microbial host for this purpose because of its fast growth rate and GRAS (generally regarded as safe) status. To date, development of this host has been impaired by the lack of genetic tools for modulating the expression of multiple genes. In this study, we present a novel two-promoter system which can be used to independently control the expression levels of two gene cassettes over a large dynamic range. Coupled with protein translation engineering and systematic media optimization, ~20 mg/L amorphadiene was produced in shake flask scale, a 40-fold improvement over the highest reported isoprenoid product yield in B. subtilis. As the tools and strategies developed here can be extended to the overproduction of other valuable metabolites, this proof-of-concept study lays the foundation for high level heterologous production of isoprenoids in Bacillus.
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Treatment of T-cell large granular lymphocyte leukemia with cyclosporine A: experience in a Chinese single institution.
Leuk. Res.
PUBLISHED: 01-17-2013
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T-cell large granular lymphocyte leukemia (T-LGLL) is a rare chronic lymphoproliferative disorder. Available reported data on the treatment regimens of this disease are variable and limited due to low number of patients. We analyzed the efficiency of cyclosporine A (CsA) in the treatment of 28 patients with T-LGLL. The overall response rate (ORR) was 82.1% with hematologic complete remission (HCR) rate of 57.1%. The median time to response (TTR) was 1.8 months and treatment duration with CsA-based regimens was 34.5 months. CsA shows low and manageable toxicity during treatment. Twenty-one patients survived with a median follow-up time of 42.0 months. Our results indicate that CsA is efficacious and safe in the treatment of T-LGLL.
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Extraction optimization of carbohydrate compound from Huangqi using orthogonal design.
Int. J. Biol. Macromol.
PUBLISHED: 01-07-2013
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The effect of extraction time, extraction temperature and time on the yield of Huangqi carbohydrate compound (HQCC) was investigated using single factor and orthogonal experiment design. The influence by the parameters on the extraction yields of carbohydrate compound decreased in the order of: C (extraction number)>A (extraction time)>B (extraction temperature) according to the R values. Based on this analysis, and considering the carbohydrate compound extraction efficiency, the cost of energy and the feasibility of experiment, the optimum conditions of extraction were therefore determined as follows: extraction time 120min, extraction temperature 80°C, and extraction number 4. Oral administration of HQCC reduced lipid peroxidation level and enhanced antioxidant enzymes activities in gastric mucosa. In addition, HQCC reduced the serum IL-8 and TNF-? levels. In conclusion, these data reveal that HQCC promotes regeneration of damaged gastric mucosa, probably through its antioxidative mechanism.
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A postmitotic function and distinct localization mechanism for centralspindlin at a stable intercellular bridge.
Dev. Biol.
PUBLISHED: 01-04-2013
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Centralspindlin, a complex composed of the subunits ZEN-4 and CYK-4, recruits and regulates proteins that modulate the actin cytoskeleton to promote cleavage furrow formation and progression during cytokinesis. The ZEN-4 subunit is a kinesin that is proposed to function primarily by bundling microtubules and promoting transport of the complex to the midzone. ZEN-4 and CYK-4 are mutually dependent for localization to the midzone during cytokinesis. Once at the midzone, the CYK-4 subunit functions to recruit actin regulators and the scaffold anillin as well as to regulate RhoA and Rac via its intrinsic GAP domain, ultimately promoting actomyosin contractile ring assembly. We have revealed a distinct mechanism for centralspindlin localization and function at a stable, postmitotic intercellular bridge in the Caenorhabditis elegans gonad. Loss of zen-4 or cyk-4 function disrupts germ cell progression postmitotically. In contrast to the localization and recruitment relationships during mitosis, centralspindlin is maintained at the intercellular bridge by anillin, and CYK-4 is localized independently of ZEN-4 but not vice versa. We present evidence that centralspindlin function at the rachis bridge involves ZEN-4 action on the microtubules as opposed to the regulation of the actin cytoskeleton mediated by CYK-4 during cytokinesis.
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High-dose cyclophosphamide compared with antithymocyte globulin for treatment of acquired severe aplastic anemia.
Exp. Hematol.
PUBLISHED: 01-03-2013
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A modified regimen of high-dose cyclophosphamide (CTX) plus cyclosporine (CsA) was adopted for patients with severe or very severe aplastic anemia, and the effectiveness was compared with a regimen of antithymocyte globulin (ATG) plus CsA. A total of 121 patients enrolled in this study received either CTX plus CsA (CTX group, 48 cases) or ATG plus CsA (ATG group, 73 cases). The early death rate was 4.2% in the CTX group and 8.2% in the ATG group, showing no significant difference (p = 0.312). The total response rate in the CTX and ATG groups was 54.2% and 57.5% at 3 months, 64.6% and 72.6% at 6 months, and 72.9% and 78.1% at 12 months, respectively (p > 0.05). The overall 5-year survival rate was 81.2% and 80.7%, and the event-free survival rate was 68.2% and 67.3% in the CTX and ATG groups, respectively (p > 0.05). The total medical cost of the CTX group was 54.8% less than that of ATG regimen (p = 0.000). In summary, treatment of severe or very severe aplastic anemia with CTX plus CsA has effectiveness that is comparable to a conventional regimen and less costly.
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Statistical Experimental Design Guided Optimization of a One-Pot Biphasic Multienzyme Total Synthesis of Amorpha-4,11-diene.
PLoS ONE
PUBLISHED: 01-01-2013
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In vitro synthesis of chemicals and pharmaceuticals using enzymes is of considerable interest as these biocatalysts facilitate a wide variety of reactions under mild conditions with excellent regio-, chemo- and stereoselectivities. A significant challenge in a multi-enzymatic reaction is the need to optimize the various steps involved simultaneously so as to obtain high-yield of a product. In this study, statistical experimental design was used to guide the optimization of a total synthesis of amorpha-4,11-diene (AD) using multienzymes in the mevalonate pathway. A combinatorial approach guided by Taguchi orthogonal array design identified the local optimum enzymatic activity ratio for Erg12:Erg8:Erg19:Idi:IspA to be 100?100?1?25?5, with a constant concentration of amorpha-4,11-diene synthase (Ads, 100 mg/L). The model also identified an unexpected inhibitory effect of farnesyl pyrophosphate synthase (IspA), where the activity was negatively correlated with AD yield. This was due to the precipitation of farnesyl pyrophosphate (FPP), the product of IspA. Response surface methodology was then used to optimize IspA and Ads activities simultaneously so as to minimize the accumulation of FPP and the result showed that Ads to be a critical factor. By increasing the concentration of Ads, a complete conversion (?100%) of mevalonic acid (MVA) to AD was achieved. Monovalent ions and pH were effective means of enhancing the specific Ads activity and specific AD yield significantly. The results from this study represent the first in vitro reconstitution of the mevalonate pathway for the production of an isoprenoid and the approaches developed herein may be used to produce other isopentenyl pyrophosphate (IPP)/dimethylallyl pyrophosphate (DMAPP) based products.
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Combinatorial Engineering of 1-Deoxy-D-Xylulose 5-Phosphate Pathway Using Cross-Lapping In Vitro Assembly (CLIVA) Method.
PLoS ONE
PUBLISHED: 01-01-2013
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The ability to assemble multiple fragments of DNA into a plasmid in a single step is invaluable to studies in metabolic engineering and synthetic biology. Using phosphorothioate chemistry for high efficiency and site specific cleavage of sequences, a novel ligase independent cloning method (cross-lapping in vitro assembly, CLIVA) was systematically and rationally optimized in E. coli. A series of 16 constructs combinatorially expressing genes encoding enzymes in the 1-deoxy-D-xylulose 5-phosphate (DXP) pathway were assembled using multiple DNA modules. A plasmid (21.6 kb) containing 16 pathway genes, was successfully assembled from 7 modules with high efficiency (2.0 x 10(3) cfu/ µg input DNA) within 2 days. Overexpressions of these constructs revealed the unanticipated inhibitory effects of certain combinations of genes on the production of amorphadiene. Interestingly, the inhibitory effects were correlated to the increase in the accumulation of intracellular methylerythritol cyclodiphosphate (MEC), an intermediate metabolite in the DXP pathway. The overexpression of the iron sulfur cluster operon was found to modestly increase the production of amorphadiene. This study demonstrated the utility of CLIVA in the assembly of multiple fragments of DNA into a plasmid which enabled the rapid exploration of biological pathways.
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Combining Genotype Improvement and Statistical Media Optimization for Isoprenoid Production in E. coli.
PLoS ONE
PUBLISHED: 01-01-2013
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Isoprenoids are a large and diverse class of compounds that includes many high value natural products and are thus in great demand. To meet the increasing demand for isoprenoid compounds, metabolic engineering of microbes has been used to produce isoprenoids in an economical and sustainable manner. To achieve high isoprenoid yields using this technology, the availability of metabolic precursors feeding the deoxyxylulose phosphate (DXP) pathway, responsible for isoprenoid biosynthesis, has to be optimized. In this study, phosphoenolpyruvate, a vital DXP pathway precursor, was enriched by deleting the genes encoding the carbohydrate phosphotransferase system (PTS) in E. coli. Production of lycopene (a C40 isoprenoid) was maximized by optimizing growth medium and culture conditions. In optimized conditions, the lycopene yield from PTS mutant was seven fold higher than that obtained from the wild type strain. This resulted in the highest reported specific yield of lycopene produced from the DXP pathway in E. coli to date (20,000 µg/g dry cell weight). Both the copy number of the plasmid encoding the lycopene biosynthetic genes and the expression were found to be increased in the optimized media. Deletion of PTS together with a similar optimization strategy was also successful in enhancing the production of amorpha-1,4-diene, a distinct C15 isoprenoid, suggesting that the approaches developed herein can be generally applied to optimize production of other isoprenoids.
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Notes on black elytron species of Pyrrhalta Joannis and the description of a new species from China (Coleoptera, Chrysomelidae, Galerucinae).
Zookeys
PUBLISHED: 01-01-2013
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Thirteen species of Pyrrhalta Joannis, 1865 with black elytron are reviewed. A key to species, photographs of aedeagus and habitus are provided. Pyrrhalta qianana sp. n. is described from Guizhou, China. Pyrrhalta martensi Medvedev & Sprecher-Uebersax, 1999 is newly recorded from China (Tibet).
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[The mutation patterns of HBV P gene and genotyping in patients with lanivudine-resistant chronic hepatitis B].
Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi
PUBLISHED: 11-25-2011
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To analyze the mutation patters of HBV P gene and genotyping of heptitis B virus in patients with chronic hepatitis B (CHB) after the emergence of drug-resistance during lamivudine (LAM) therapy.
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[Significance of serum golgi protein 73 (GP73), alpha-fetoprotein (AFP) and lectin-reactive alpha-fetoprotein (AFP-L3) expresssion in primary hepatic carcinoma].
Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi
PUBLISHED: 11-22-2011
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To explore the alone and joint diagnostic value of serum golgi protein 73 (GP73), alpha-fetoprotein (AFP) and the percentage of lectin-reactive aipha-fetoprotein (AFP-L3) of primary hepatic carcinoma (PHC), and provide a novel method for diagnosis for PHC and screening for high-risk population.
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[Diagnosis and surgical treatment for mediastinal hemangioma and lymphangioma].
Zhonghua Yi Xue Za Zhi
PUBLISHED: 11-19-2011
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To review the experience of diagnosis and surgical treatment of the primary mediastinal hemangioma and lymphangioma.
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Utility of squamous cell carcinoma antigen, carcinoembryonic antigen, Cyfra 21-1 and neuron specific enolase in lung cancer diagnosis: a prospective study from China.
Chin. Med. J.
PUBLISHED: 11-18-2011
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Early detection and diagnosis is urgent for the sake of effective treatment strategy for lung cancer. However, a convenient, economical and relatively precise method is not available. We here report a prospective study to find the possible value of the combined use of four popular tumor markers in the early diagnosis of lung cancer among patients with suspicious nodules in the lung.
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[Preparation and luminescence characteristics of white-light emitting Ca9.95-xNa0.75K0.25(PO4)7: Eu0.05(2+), Mn(x)2+ phosphors].
Guang Pu Xue Yu Guang Pu Fen Xi
PUBLISHED: 10-20-2011
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The Ca9.95-x Na0.75 K0.25 (PO4)7: Eu.0.05(2+), Mn(x)2+ (x = 0, 0.1, 0.2, 0.3, 0.4, 0.5, 0.6 and 0.7) phosphors were synthesized by high temperature solid-state reaction, and their phase composition and fluorescence emission properties were studied. Due to the existence of double phases with similar crystal structure, the 5d-4f transition of Eu2+ ions in the phosphors emits a fluorescence of wide wavelength with peaks located at 491 nm and 540 nm respectively. The energy transfer between Eu2+ and Mn2+, together with the occupation of Mn2+ ions at the eight coordination sites in the phosphors, makes the 4T1 (4G)-6A1 (6S) transition of Mn2+ ions eimit red emission peaked at 635 nm. The combination of fluorescence emtted by Mn2+ and Eu2+ ions results in the emission of white light with color coordinates (0.3335, 0.2924), (0.3999, 0.3179) and (0.3307, 0.2564). The nearly pure white light emitting makes the phosphors show great application potential in the white light-emitting LEDs.
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[Diagnosis and surgical treatment of congenital bronchoesophageal fistula in adult].
Zhonghua Wai Ke Za Zhi
PUBLISHED: 09-15-2011
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To explore the clinical characteristics, diagnosis and surgical treatment of adult congenital bronchoesophageal fistula.
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[Effects of concentration of cyclosporine A on the early response to immunosuppressive therapy in severe aplastic anemia].
Zhonghua Xue Ye Xue Za Zhi
PUBLISHED: 08-21-2011
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To evaluate the effects of cyclosporine A (CsA) whole-blood concentration on the early response to immunosuppressive therapy (IST) in severe and very severe aplastic anemia (SAA/VSAA).
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[Therapeutic effect of 125I-103Pd hybrid radioactive seeds on pulmonary carcinomas in BALB/c-nu mice].
Zhonghua Yi Xue Za Zhi
PUBLISHED: 07-16-2011
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To prepared (125)I-(103)Pd hybrid radioactive seeds and to explore their therapeutic effect on pulmonary carcinomas.
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[Expression of dopamine receptor D2 in pulmonary carcinoma cells and effects of dopamine on the cell apoptosis].
Nan Fang Yi Ke Da Xue Xue Bao
PUBLISHED: 06-22-2011
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To detect the expression of dopamine receptor D2 in different pulmonary carcinoma cells and investigate the effect of dopamine in inducing apoptosis of A549 cells.
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[Reoperative valve surgery after open-heart valve surgery: a report of 155 cases].
Zhong Nan Da Xue Xue Bao Yi Xue Ban
PUBLISHED: 06-21-2011
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To summarize the characteristics of reoperative valve surgery after previous open-heart valve surgery.
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Thirty-day in-vivo performance of a wearable artificial pump-lung for ambulatory respiratory support.
Ann. Thorac. Surg.
PUBLISHED: 05-04-2011
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The purpose of this study was to evaluate the long-term in-vivo hemodynamics, gas transfer, and biocompatibility of an integrated artificial pump-lung (APL) developed for ambulatory respiratory support.
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Phylogenetically diverse cultivable fungal community and polyketide synthase (PKS), non-ribosomal peptide synthase (NRPS) genes associated with the South China Sea sponges.
Microb. Ecol.
PUBLISHED: 04-10-2011
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Compared with sponge-associated bacteria, the phylogenetic diversity of fungi in sponge and the association of sponge fungi remain largely unknown. Meanwhile, no detection of polyketide synthase (PKS) or non-ribosomal peptide synthase (NRPS) genes in sponge-associated fungi has been attempted. In this study, diverse and novel cultivable fungi including 10 genera (Aspergillus, Ascomycete, Fusarium, Isaria, Penicillium, Plectosphaerella, Pseudonectria, Simplicillium, Trichoderma, and Volutella) in four orders (Eurotiales, Hypocreales, Microascales, and Phyllachorales) of phylum Ascomycota were isolated from 10 species marine sponges in the South China Sea. Eurotiales and Hypocreales fungi were suggested as sponge generalists. The predominant isolates were Penicillium and Aspergillus in Eurotiales followed by Volutella in Hypocreales. Based on the conserved Beta-ketosynthase of PKS and A domain of NRPS, 15 polyketide synthases, and four non-ribosomal peptides synthesis genes, including non-reducing and reducing PKSs and hybrid PKS-NRPS, were detected in these fungal isolates. A lateral gene transfer event was indicated in the comparison between the phylogenetic diversity of 18S rRNA genes and ?-ketoacyl synthase domain sequences. Some fungi, especially those with PKS or NRPS genes, showed antimicrobial activity against P. fluorescens, S. aureus and B. subtilis. It was the first time to investigate PKS and NRPS genes in sponge-associated fungi. Based on the detected antibiotics biosynthesis-related PKS and NRPS genes and antimicrobial activity, the potential ecological role of sponge-associated fungi in the chemical defense for sponge host was suggested. This study extended our knowledge of sponge-associated fungal phylogenetic diversity and their potential roles in the chemical defense.
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[Combination of rabbit antithymocyte globulin and cyclosporine A as first-line therapy for adult severe aplastic anemia].
Zhonghua Xue Ye Xue Za Zhi
PUBLISHED: 03-25-2011
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To analyze the efficacy and side-effects of combination of rabbit antithymocyte globulin (ATG) and cyclosporine A (CsA) as the first-line immunosuppressive therapy (IST) for adult severe aplastic anemia (SAA) patients.
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Novel reference genes for quantifying transcriptional responses of Escherichia coli to protein overexpression by quantitative PCR.
BMC Mol. Biol.
PUBLISHED: 01-21-2011
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Accurate interpretation of quantitative PCR (qPCR) data requires normalization using constitutively expressed reference genes. Ribosomal RNA is often used as a reference gene for transcriptional studies in E. coli. However, the choice of reliable reference genes has not been systematically validated. The objective of this study is to identify a set of reliable reference genes for transcription analysis in recombinant protein over-expression studies in E. coli.
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Congenital bronchoesophageal fistula in adults.
World J. Gastroenterol.
PUBLISHED: 01-04-2011
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To study the clinical characteristics, diagnosis and surgical treatment of congenital bronchoesophageal fistulae in adults.
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[Pathological correlation of dynamic magnetic resonance findings for chromophobe cell renal carcinoma].
Zhonghua Yi Xue Za Zhi
PUBLISHED: 12-18-2010
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To explore the dynamic magnetic resonance (MR) imaging findings of chromophobe cell renal carcinoma and evaluate its pathological correlation.
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[Prognostic analysis of partial atrium or large blood vessel resection for treatment of locally advanced lung cancer].
Nan Fang Yi Ke Da Xue Xue Bao
PUBLISHED: 11-25-2010
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To explore the value of partial atrium or large blood vessel resection for the treatment of locally advanced lung cancer.
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[OATP 1B1 T521C/A388G is an important polymorphism gene related to neonatal hyperbilirubinemia].
Zhonghua Er Ke Za Zhi
PUBLISHED: 11-25-2010
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Multiple genetic and environmental factors contribute to the onset of many human diseases, such as neonatal hyperbilirubinemia. OATP 1B1 is an important polymorphism gene which transmembrane transports unconjugated bilirubin(UCB). Genetic polymorphisms that affect the functionality of the protein may potentially lead to altered transport characteristics. The T521C/A388G polymorphism of this gene has been reported to considerably reduce the transporting property of drugs like pravastatin, and may be involved in the membrane translocation of bilirubin. Some studies have shown that OATP 1B1 mediates bilirubin uptake from blood into the liver, and the OATP 1B1 polymorphism is a likely mechanism explaining the differences of bilirubin level in peripheral blood. The aim of this study was to evaluate the relationship between OATP 1B1 polymorphisms and neonatal hyperbilirubinemia.
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Preliminary study on MR colonography with air enema in detection of colorectal neoplasms.
Chin. Med. J.
PUBLISHED: 11-02-2010
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The few studies on MR colonography with air enema involved feasibility of bowel distention and imaging quality and lacked detection sensitivity of colorectal neoplasms. The purpose of this prospective study was to assess the detection sensitivity of colorectal neoplasms with the three-dimensional Fourier transform fast spoiled gradient-recalled MR colonography with air enema.
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Effects and mechanisms of non-restrictive external stent for prevention of vein graft restenosis in a rabbit model.
Chin. Med. J.
PUBLISHED: 11-02-2010
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Among various treatments preventing vein graft restenosis, external stent is receiving more and more attention. This study aimed to investigate the effect of non-restrictive external stent on the prevention of vein graft restenosis and the potential mechanisms of platelet-derived growth factor (PDGF) in the process of restenosis.
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Structural insights into the catalytic mechanism of the yeast pyridoxal 5-phosphate synthase Snz1.
Biochem. J.
PUBLISHED: 10-06-2010
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In most eubacteria, fungi, apicomplexa, plants and some metazoans, the active form of vitamin B6, PLP (pyridoxal 5-phosphate), is de novo synthesized from three substrates, R5P (ribose 5-phosphate), DHAP (dihydroxyacetone phosphate) and ammonia hydrolysed from glutamine by a complexed glutaminase. Of the three active sites of DXP (deoxyxylulose 5-phosphate)independent PLP synthase (Pdx1), the R5P isomerization site has been assigned, but the sites for DHAP isomerization and PLP formation remain unknown. In the present study, we present the crystal structures of yeast Pdx1/Snz1, in apo-, G3P (glyceraldehyde 3-phosphate)- and PLP-bound forms, at 2.3, 1.8 and 2.2 Å (1 Å=0.1 nm) respectively. Structural and biochemical analysis enabled us to assign the PLP-formation site, a G3P-binding site and a G3P-transfer site. We propose a putative catalytic mechanism for Pdx1/Snz1 in which R5P and DHAP are isomerized at two distinct sites and transferred along well-defined routes to a final destination for PLP synthesis.
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[The significance of hematopoietic cell genetic instability in aplastic anemia.].
Zhonghua Xue Ye Xue Za Zhi
PUBLISHED: 06-01-2010
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To evaluate bone marrow hematopoietic cells genetic instability (BMHCGI) in patients with aplastic anemia (AA) and to explore its influence on immunosupressive therapy for AA and significance on late clonal hematologic disorders.
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Nano-structured manganese oxide as a cathodic catalyst for enhanced oxygen reduction in a microbial fuel cell fed with a synthetic wastewater.
Water Res.
PUBLISHED: 05-23-2010
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Microbial fuel cells (MFCs) provide new opportunities for the simultaneous wastewater treatment and electricity generation. Enhanced oxygen reduction capacity of cost-effective metal-based catalysts in an air cathode is essential for the scale-up and commercialization of MFCs in the field of wastewater treatment. We demonstrated that a nano-structured MnO(x) material, prepared by an electrochemically deposition method, could be an effective catalyst for oxygen reduction in an MFC to generate electricity with the maximum power density of 772.8 mW/m(3) and remove organics when the MFC was fed with an acetate-laden synthetic wastewater. The nano-structured MnO(x) with the controllable size and morphology could be readily obtained with the electrochemical deposition method. Both morphology and manganese oxidation state of the nano-scale catalyst were largely dependent on the electrochemical preparation process, and they governed its catalytic activity and the cathodic oxygen reduction performance of the MFC accordingly. Furthermore, cyclic voltammetry (CV) performed on each nano-structured material suggests that the MnO(x) nanorods had an electrochemical activity towards oxygen reduction reaction via a four-electron pathway in a neutral pH solution. This work provides useful information on the facile preparation of cost-effective cathodic catalysts in a controllable way for the single-chamber air-cathode MFC for wastewater treatment.
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[Comparison of the biodistribution and PET imaging with (11)C-PDT and (18)F-FDG in the mouse model of lung adenocarcinoma].
Zhonghua Zhong Liu Za Zhi
PUBLISHED: 04-21-2010
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The objective of this study was to compare the biodistribution and PET imaging of (11)C-PDT and (18)F-FDG in a mouse model of lung adenocarcinoma, and to evaluate the value of (11)C-PDT as a new tracer for PET imaging of lung cancer.
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[Computed tomographic angiography for evaluation of the relationship between coronary artery stenosis and head and neck artery stenosis].
Zhongguo Yi Xue Ke Xue Yuan Xue Bao
PUBLISHED: 03-26-2010
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To evaluate the relationship between coronary artery stenosis and internal carotid artery, vertebral and basilar artery stenosis using computed tomographic angiography.
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[Comparison of prospectively electrocardiographic triggered spiral and sequential acquisition for coronary computed tomographic angiography using dual-source computed tomography].
Zhongguo Yi Xue Ke Xue Yuan Xue Bao
PUBLISHED: 03-26-2010
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To compare the image quality and radiation dose of prospectively electrocardiogram (ECG) -triggered spiral and sequential acquisition for coronary computed tomographic angiography by dual-source computed tomography.
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[Coronary computed tomographic angiography using low-dose prospectively electrocardiographic triggered high-pitch spiral acquisition by dual-source computed tomography: image quality and radiation dose].
Zhongguo Yi Xue Ke Xue Yuan Xue Bao
PUBLISHED: 03-26-2010
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To evaluate image quality (IQ) and radiation exposure of coronary computed tomographic angiography (CTA) with prospectively electrocardiographic (ECG) triggered high-pitch spiral acquisition using dual source CT.
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L-carnitine and taurine synergistically inhibit the proliferation and osteoblastic differentiation of vascular smooth muscle cells.
Acta Pharmacol. Sin.
PUBLISHED: 02-15-2010
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To investigate the synergistic action of L-carnitine (LC) and taurine (TAU) on the proliferation and osteoblastic differentiation of vascular smooth muscle cells (VSMCs).
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Movers and shakers or anchored: Caenorhabditis elegans nuclei achieve it with KASH/SUN.
Dev. Dyn.
PUBLISHED: 01-29-2010
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The invariant cell division patterns that characterize Caenorhabditis elegans development make it an ideal system to study the mechanisms that control nuclear movement and positioning. Forward genetic screens in this system allowed identification of the key molecular machinery for connecting the nucleus to the cytoskeleton; pairs of protein partners, consisting of a KASH domain protein and a SUN domain protein, bridge the nuclear envelope to connect the nucleus to cytoskeletal components. The C. elegans genome encodes several KASH/SUN pairs, and mutant phenotypes as well as tissue-specific expression patterns suggest a diversity of functions. These functions include moving the nucleus but have been extended to effects on the chromosomes inside the nucleus as well. We review the impact of the C. elegans system in pioneering this field as well as the functions of these KASH/SUN protein pairs across spatial and temporal C. elegans development.
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Survival benefit of neoadjuvant chemotherapy in non-small cell lung cancer: an updated meta-analysis of 13 randomized control trials.
J Thorac Oncol
PUBLISHED: 01-29-2010
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The survival effectiveness of neoadjuvant chemotherapy in non-small cell lung cancer (NSCLC) is still unclear based on the study of most up-to-date literatures. This article contributes to this problem by conducting an updated meta-analysis.
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[CT and MRI imaging features of peripheral primitive neuroectodermal tumors].
Zhonghua Zhong Liu Za Zhi
PUBLISHED: 12-22-2009
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To investigate the CT and MRI features of peripheral primitive neuroectodermal tumors (pPNETs) and evaluate its diagnostic value.
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[Clinical and hematological features of congenital dyserythropoietic anemia type I].
Zhonghua Xue Ye Xue Za Zhi
PUBLISHED: 12-03-2009
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To analyze the clinical and laboratory features of patients with congenital dyserythropoietic anemia type I (CDA-I), and improve the clinical diagnostic accuracy.
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[Expression of EYA2 in non-small cell lang cancer].
Zhonghua Zhong Liu Za Zhi
PUBLISHED: 12-03-2009
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To identify the expression of Drosophila Eyes Absent Homologue 2 (EYA2) in non-small cell lung cancer (NSCLC) and to investigate its correlation with clinical parameters.
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[Combination of rabbit antithymocyte globulin plus cyclosporin A as first-line immunosuppressive therapy for the childhood with severe aplastic anemia.].
Zhonghua Xue Ye Xue Za Zhi
PUBLISHED: 09-30-2009
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To analyse the efficacy and side-effects of rabbit antithymocyte globulin (ATG) and cyclosporin A (CsA) as the first-line therapy for childhood severe aplastic anemia (SAA).
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[A new algorithm for ultrasound image speckle suppression].
Zhongguo Yi Liao Qi Xie Za Zhi
PUBLISHED: 09-24-2009
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Ultrasound image has a lot of speckle noise, which brings great difficulties to the feature extraction, recognition and analysis. Especially in the edge extraction, the conventional extraction algorithms are difficult to achieve the desired results because of the speckle noise. To solve this problem, an algorithm based on the anisotropic diffusion equation is presented. The new algorithm combines the robust estimation and considers the characteristic of the speckle noise, so it can suppress the speckle noise effectively and be more robust, thus the edge details of the ultrasound image can be preserved even enhanced, which can provide effective safeguard for the following edge extraction. Furthermore, the paper proposes a new method to compute speckle scale coefficients automatically, which reduces the influence of the human beings, and enhances the stability of the algorithm.
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[The clinical and laboratory characteristics of T cell large granular lymphocyte leukemia].
Zhonghua Xue Ye Xue Za Zhi
PUBLISHED: 08-01-2009
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To analyze the characteristics of T-cell large granular lymphocyte leukemia (T-LGLL).
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What is Visualize?

JoVE Visualize is a tool created to match the last 5 years of PubMed publications to methods in JoVE's video library.

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We use abstracts found on PubMed and match them to JoVE videos to create a list of 10 to 30 related methods videos.

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In developing our video relationships, we compare around 5 million PubMed articles to our library of over 4,500 methods videos. In some cases the language used in the PubMed abstracts makes matching that content to a JoVE video difficult. In other cases, there happens not to be any content in our video library that is relevant to the topic of a given abstract. In these cases, our algorithms are trying their best to display videos with relevant content, which can sometimes result in matched videos with only a slight relation.