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Find video protocols related to scientific articles indexed in Pubmed.
Electronic structures at the interface between Au and CH3NH3PbI3.
Phys Chem Chem Phys
PUBLISHED: 11-20-2014
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The electronic properties of interfaces formed between Au and organometal triiodide perovskite (CH3NH3PbI3) are investigated using ultraviolet photoemission spectroscopy (UPS), inverse photoemission spectroscopy (IPES) and X-ray photoemission spectroscopy (XPS). It is found that the CH3NH3PbI3 film coated onto the substrate of poly(3,4-ethylenedioxythiophene) poly(styrenesulfonate) (PEDOT:PSS)/indium tin oxide (ITO) by a two-step method presents n-type semiconductor behavior, with a band gap of 1.7 eV and a valence band (VB) edge of 1.0 eV below the Fermi energy (EF). An interface dipole of 0.1 eV is observed at the CH3NH3PbI3/Au interface. The energy levels of CH3NH3PbI3 shift upward by ca. 0.4 eV with an Au coverage of 64 Å upon it, resulting in band bending, hence a built-in field in CH3NH3PbI3 that encourages hole transport to the interface. Hole accumulation occurs in the vicinity of the interface, facilitating the hole transfer from CH3NH3PbI3 to Au. Furthermore, the shift of the VB maximum of CH3NH3PbI3 toward the EF indicates a decrease of energy loss as holes transfer from CH3NH3PbI3 to Au.
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Medical Harm: Patient Perceptions and Follow-up Actions.
J Patient Saf
PUBLISHED: 11-15-2014
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Much research has been conducted to describe medical mistakes resulting in patient harm using databases that capture these events for medical organizations. The objective of this study was to describe patients' perceptions regarding disclosure and their actions after harm.
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Two congenital coronary abnormalities affecting heart function: anomalous origin of the left coronary artery from the pulmonary artery and congenital left main coronary artery atresia.
Chin. Med. J.
PUBLISHED: 11-11-2014
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The anomalous origin of the left coronary artery (LCA) from the pulmonary artery (ALCAPA) and congenital left main coronary artery atresia (CLMCA-A) are two kinds of very rare coronary heart diseases which affect heart function profoundly. This study aimed to retrospectively illustrate the clinical features and therapy experience of ALCAPA and CLMCA-A patients.
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[Chinese guidelines for data processing and analysis concerning the International Physical Activity Questionnaire].
Zhonghua Liu Xing Bing Xue Za Zhi
PUBLISHED: 11-08-2014
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To introduce standard methods of scoring the data derived from both short and long form instruments of the International Physical Activity Questionnaire (IPAQ). The introduction part consists of a set of domains which include the structure of questionnaire, principles of data cleaning, calculation methods and grouping rules. Through practical experience, a specific calculation process of IPAQ-long based on the standard methods was showed.
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[Using biomass fuel at home and the development of hypertension in adult women living in rural areas of Guangdong: a multilevel model analysis].
Zhonghua Liu Xing Bing Xue Za Zhi
PUBLISHED: 11-08-2014
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To understand the situation of biomass fuel use in rural Guangdong, and how it affecting the prevalence of hypertension in adult women.
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[Regional differences in patterns of alcohol consumption: findings from the China Kadoorie Biobank study on half a million people from 10 regions].
Zhonghua Liu Xing Bing Xue Za Zhi
PUBLISHED: 11-08-2014
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To describe the regional differences on patterns of alcohol consumption across 10 study in the China Kadoorie Biobank (CKB) areas involving half a million adults.
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Brazilin isolated from Caesalpinia. sappan suppresses nuclear envelope reassembly by inhibiting BAF phosphorylation.
J. Pharmacol. Exp. Ther.
PUBLISHED: 11-06-2014
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To date, many anticancer drugs have been developed by directly or indirectly targeting microtubules, which are involved in cell division. Although this approach has yielded many anticancer drugs, these drugs possess undesirable side effects. An alternative strategy is needed, and targeting mitotic exit may be one alternative approach. Localization of phosphorylated barrier-to-autointegration factor (BAF) to the chromosomal core region is essential for nuclear envelope compartment re-localization. In this study, we isolated brazilin from Caesalpinia sappan Leguminosae and demonstrated that it inhibited BAF phosphorylation in vitro and in vivo. Moreover, we demonstrated direct binding between brazilin and BAF. The inhibition of BAF phosphorylation induced abnormal nuclear envelope reassembly and cell death, indicating that perturbation of nuclear envelope reassembly could be a novel approach to anticancer therapy. We propose that brazilin isolated from C. sappan may be a new anticancer drug candidate that induces cell death by inhibiting vaccinia-related kinase 1 (VRK1)-mediated BAF phosphorylation.
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Non-small cell lung cancer with EML4-ALK translocation in Chinese male never-smokers is characterized with early-onset.
BMC Cancer
PUBLISHED: 10-30-2014
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The translocations of the anaplastic lymphoma kinase (ALK) gene with the echinoderm microtubule-associated protein-like 4 (EML4) gene on chromosome 2p have been identified in non-small-cell lung cancers (NSCLCs) as oncogenic driver mutations. It has been suggested that EML4-ALK fusion is associated with the resistance in NSCLCs to epidermal growth factor receptor tyrosine kinase inhibitors (EGFR TKIs), such as gefitinib and erlotinib. In contrast, ALK tyrosine kinase inhibitor (ALK TKI) crizotinib has shown superior effects in combating NSCLCs with EML4-ALK. Thus, characterization of EML4-ALK fusion genes and clinical features of resulting carcinomas would be a great benefit to disease diagnosis and designing customized treatment plans. Studies have suggested that EML4-ALK translocation occurs more frequently in never-smokers with NSCLC, especially in female patients. However, it is not clear whether this is the case in male patients, too. In this study, we have determined the frequency of EML4-ALK translocation in male never-smokers with NSCLC in a cohort of Chinese patients. The clinical features associated with EML4-ALK translocation were also investigated.
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[Effect of peer education on postoperative rehabilitation of patients with laryngeal cancer].
Zhonghua Er Bi Yan Hou Tou Jing Wai Ke Za Zhi
PUBLISHED: 10-30-2014
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To evaluate the effect of peer education on postoperative rehabilitation of patients with laryngeal cancer.
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[NK4 growth inhibition of human Raji lymphoma xenografts by competitive interrupting HGF/Met signal pathway].
Zhonghua Bing Li Xue Za Zhi
PUBLISHED: 10-28-2014
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To observe the inhibition of NK4 protein in the proliferation of human Raji lymphoma xenografts in nude mice, and to explore its molecular mechanism.
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[Comparative study on the efficacy of Hyper CVAD/MA regimen and CHOP or CHOP like regimen in the treatment of primary peripheral T cell lymphoma].
Zhonghua Xue Ye Xue Za Zhi
PUBLISHED: 10-24-2014
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To evaluate the curative efficacy and safety of two regimens, Hyper CVAD/MA and CHOP/CHOP, in the treatment of primary peripheral T cell lymphoma (PTCL).
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[Immunosuppressive effects of fetal bone marrow derived mesenchymal stem cells on in vitro proliferation of adult peripheral lymphocyte and expression of immune-related factors].
Zhonghua Xue Ye Xue Za Zhi
PUBLISHED: 10-24-2014
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To investigate the potential immunomodulatory properties of fetal bone marrow derived mesenchymal stem cells (FBM- MSCs).
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Low Overpotential in Vacancy-Rich Ultrathin CoSe2 Nanosheets for Water Oxidation.
J. Am. Chem. Soc.
PUBLISHED: 10-22-2014
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According to Yang Shao-Horn's principle, CoSe2 is a promising candidate as an efficient, affordable, and sustainable alternative electrocatalyst for the oxygen evolution reaction, owing to its well-suited electronic configuration of Co ions. However, the catalytic efficiency of pure CoSe2 is still far below what is expected, because of its poor active site exposure yield. Herein, we successfully overcome the disadvantage of insufficient active sites in bulk CoSe2 by reducing its thickness into the atomic scale rather than any additional modification (such as doping or hybridizing with graphene or noble metals). The positron annihilation spectrometry and XAFS spectra provide clear evidence that a large number of VCo? vacancies formed in the ultrathin nanosheets. The first-principles calculations reveal that these VCo? vacancies can serve as active sites to efficiently catalyze the oxygen evolution reaction, manifesting an OER overpotential as low as 0.32 V at 10 mA cm(-2) in pH 13 medium, which is superior to the values for its bulk counterparts as well as those for the most reported Co-based electrocatalysts. Considering the outstanding performance of the simple, unmodified ultrathin CoSe2 nanosheets as the only catalyst, further improvement of the catalytic activity is expected when various strategies of doping or hybridizing are used. These results not only demonstrate the potential of a notable, affordable, and earth-abundant water oxidation electrocatalyst based on ultrathin CoSe2 nanosheets but also open up a promising avenue into the exploration of excellent active and durable catalysts toward replacing noble metals for oxygen electrocatalysis.
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[Incident and related risk factors of hypertension in women with a history of preeclampsia].
Zhonghua Xin Xue Guan Bing Za Zhi
PUBLISHED: 10-21-2014
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To investigate the prevalence of hypertension in women with a history of preeclampsia (PE) and to estimate related risk factors.
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[Efficacy of intravascular ultrasound guided rotational atherectomy for heavily calcified coronary lesions].
Zhonghua Xin Xue Guan Bing Za Zhi
PUBLISHED: 10-21-2014
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To evaluate the efficacy of intravascular ultrasound guided tranradial rotational atherectomy (RA) followed by drug eluting stent (DES) implantation in treating patients with heavily calcified coronary lesions.
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[The expression and significance of beta2-AR and VEGFR-2 in infantile hemangioma].
Zhonghua Zheng Xing Wai Ke Za Zhi
PUBLISHED: 10-18-2014
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To investigate the significance of beta-adrenergic receptor 2 (beta2-AR) and vascular endothelial growth factor-2 (VEGFR-2) in the occurrence and development of infantile hemangioma through detecting the expression of beta2-AR and VEGFR-2 in the different stages of infantile hemangiomas. Methods According to the Mulliken's classification standard, we classified the specimens as proliferating group (32 cases), involuting group (17 cases) and involuted group (11 cases). Normal skin tissue surrounding the hemangioma from 7 cases were chosen as control group. The expression of beta2-AR and VEGFR-2 was detected by immunohistochemical technique in proliferating hemangioma, involuting hemangioma, involuted hemangioma. The mean optical density was measured by image analysis system (Image Pro Plus 6.0) and SPSS 16.0 software was applied for statistical analysis.
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Regional white matter abnormalities in drug-naive, first-episode schizophrenia Patients and their healthy unaffected siblings.
Aust N Z J Psychiatry
PUBLISHED: 10-17-2014
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Shared neuropathological features between schizophrenia patients and their siblings may represent intermediate phenotypes of schizophrenia and can be used to investigate genetic susceptibility to the illness. This study aimed to discover regional white matter abnormalities in first-episode schizophrenia (FES) patients and their unaffected siblings compared to healthy subjects in the Chinese Han population using optimized Voxel-Based Morphometry (VBM).
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[Exposure risk assessment of plasticizer in dietary food in Xiamen].
Zhonghua Yu Fang Yi Xue Za Zhi
PUBLISHED: 10-15-2014
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To understand the dietary consumption of residents in Xiamen and the content of phthalic acid esters (PAEs) in food, and to assess the plasticizer exposure risk of diet in Xiamen.
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6-O-Sulfated Chitosan Promoting the Neural Differentiation of Mouse Embryonic Stem Cells.
ACS Appl Mater Interfaces
PUBLISHED: 10-11-2014
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Embryonic stem cells (ESCs) can be induced to differentiate into nerve cells, endowing them with potential applications in the treatment of neurological diseases and neural repair. In this work, we report for the first time that sulfated chitosan can promote the neural differentiation of ESCs. As a type of sulfated glycosaminoglycan analog, sulfated chitosan with well-defined sulfation sites and a controlled degree of sulfation (DS) were prepared through simple procedures and the influence of sulfated glycosaminoglycan on neural differentiation of ESCs was investigated. Compared with other sulfation sites, 6-O-sulfated chitosan showed the most optimal effects. By monitoring the expression level of neural differentiation markers using immunofluorescence staining and PCR, it was found that neural differentiation was better enhanced by increasing the DS of 6-O-sulfated chitosan. However, increasing the DS by introducing another sulfation site in addition to the 6-O site to chitosan did not promote neural differentiation as much as 6-O-sulfated chitosan, indicating that compared with DS, the sulfation site is more important. Additionally, the optimal concentration and incubation time of 6-O-sulfated chitosan were investigated. Together, our results indicate that the sulfate site and the molecular structure in a sulfated polysaccharide are very important for inducing the differentiation of ESCs. Our findings may help to highlight the role of sulfated polysaccharide in inducing the neural differentiation of ESCs.
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[Effect of ? -1,2 fucosyltransferase gene 682A> G and 547_552delAG mutations on the activity of fucosyltransferase].
Zhonghua Yi Xue Yi Chuan Xue Za Zhi
PUBLISHED: 10-10-2014
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To explore the effect of ? -1,2 fucosyltransferase (FUT1) gene 682A> G and 547_552delAG mutations on the expression of FUT1 mRNA and activity of ? -1,2 fucosyltransferase.
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[Screening of common deafness gene mutations in 17 000 Chinese newborns from Chengdu based on microarray analysis].
Zhonghua Yi Xue Yi Chuan Xue Za Zhi
PUBLISHED: 10-10-2014
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To achieve early diagnosis for inheritable hearing loss and determine carrier rate of deafness causing gene mutations in order to provide information for premarital, prenatal and postnatal genetic counseling.
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Manifold Adaptive Label Propagation for Face Clustering.
IEEE Trans Cybern
PUBLISHED: 10-08-2014
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In this paper, a novel label propagation (LP) method is presented, called the manifold adaptive label propagation (MALP) method, which is to extend original LP by integrating sparse representation constraint into regularization framework of LP method. Similar to most LP, first of all, MALP also finds graph edges from given data and gives weights to the graph edges. Our goal is to find graph weights matrix adaptively. The key advantage of our approach is that MALP simultaneously finds graph weights matrix and predicts the label of unlabeled data. This paper also derives efficient algorithm to solve the proposed problem. Extensions of our MALP in kernel space and robust version are presented. The proposed method has been applied to the problem of semi-supervised face clustering using the well-known ORL, Yale, extended YaleB, and PIE datasets. Our experimental evaluations show the effectiveness of our method.
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Nanotube Li2MoO4: a novel and high-capacity material as a lithium-ion battery anode.
Nanoscale
PUBLISHED: 10-03-2014
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Carbon-coated Li2MoO4 hexagonal hollow nanotubes were fabricated via a facile sol-gel method involving the solution synthesis of Li2MoO4 with subsequent annealing under an inert atmosphere to decompose the organic carbon source. To the best of our knowledge, this is the first report on the synthesis of Li2MoO4 nanotubes. More significantly, we have found that Li2MoO4 can be used as an anode material for lithium-ion batteries (LIBs). When evaluated as an anode material, the carbon-coated Li2MoO4 hollow nanotubes show an excellent electrochemical performance with a high reversible capacity (?550 mA h g(-1)) after 23 cycles, good rate capability and cycling stability. Meanwhile, carbon-free Li2MoO4 sample, fabricated via a solid state reaction, was also prepared for comparison. The Li storage mechanism has been investigated in-detail by advanced XPS, in situ XRD and HRTEM.
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Differentiation of neuromyelitis optica from multiple sclerosis in a cohort from the mainland of China.
Chin. Med. J.
PUBLISHED: 10-01-2014
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Although there were criteria for diagnosis of neuromyelitis optica (NMO) and multiple sclerosis (MS), it is still difficult to differentiate NMO from MS, due to the overlapping clinical manifestations. Therefore it is necessary to characterize clinical features of NMO and MS patients in the mainland of China, to simplify the process of disease diagnosis, and to identify criteria for the differential diagnosis of NMO and MS.
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[The relationship between calcaneus bone mineral density and metabolic syndrome].
Zhonghua Nei Ke Za Zhi
PUBLISHED: 09-30-2014
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To evaluate the association between calcaneus bone mineral density (BMD) and metabolic syndrome (MS).
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[Clinical characteristics of 297 newly diagnosed Chinese HIV/AIDS patients].
Zhonghua Nei Ke Za Zhi
PUBLISHED: 09-30-2014
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To determine the clinical characteristics of HIV infected patients in China in order to improve early recognition and diagnosis of AIDS.
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Proteomic analysis of rhein-induced cyt: ER stress mediates cell death in breast cancer cells.
Mol Biosyst
PUBLISHED: 09-27-2014
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Rhein is a natural product purified from herbal plants such as Rheum palmatum, which has been shown to have anti-angiogenesis and anti-tumor metastasis properties. However, the biological effects of rhein on the behavior of breast cancers are not completely elucidated. To evaluate whether rhein might be useful in the treatment of breast cancer and its cytotoxic mechanism, we analyzed the impact of rhein treatment on differential protein expression as well as redox regulation in a non-invasive breast cancer cell line, MCF-7, and an invasive breast cancer cell line, MDA-MB-231, using lysine- and cysteine-labeling two-dimensional difference gel electrophoresis (2D-DIGE) combined with MALDI-TOF/TOF mass spectrometry. This proteomic study revealed that 73 proteins were significantly changed in protein expression; while 9 proteins were significantly altered in thiol reactivity in both MCF-7 and MDA-MB-231 cells. The results also demonstrated that rhein-induced cytotoxicity in breast cancer cells mostly involves dysregulation of cytoskeleton regulation, protein folding, the glycolysis pathway and transcription control. A further study also indicated that rhein promotes misfolding of cellular proteins as well as unbalancing of the cellular redox status leading to ER-stress. Our work shows that the current proteomic strategy offers a high-through-put platform to study the molecular mechanisms of rhein-induced cytotoxicity in breast cancer cells. The identified differentially expressed proteins might be further evaluated as potential targets in breast cancer therapy.
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[Efficacy of retropharyngeal nodes dissection in hypopharyngeal cancer].
Zhonghua Er Bi Yan Hou Tou Jing Wai Ke Za Zhi
PUBLISHED: 09-27-2014
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To investigate the necessity and feasibility of planned dissection of the retropharyngeal lymph nodes (RPLN) in advanced hypopharyngeal cancer.
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[Prognostic factors affecting results of comprehensive treatment of hypopharyngeal carcinoma].
Zhonghua Er Bi Yan Hou Tou Jing Wai Ke Za Zhi
PUBLISHED: 09-27-2014
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To investigate the efficacy of surgery followed by adjuvant radiotherapy and the prognostic factors affecting the results of treatment in hypopharyngeal carcinoma.
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Extracting and Selecting Distinctive EEG Features for Efficient Epileptic Seizure Prediction.
IEEE J Biomed Health Inform
PUBLISHED: 09-24-2014
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This paper presents compact yet comprehensive feature representations for the Electroencephalogram (EEG) signal to achieve efficient epileptic seizure prediction performance. The initial EEG feature vectors are formed by acquiring the dominant amplitude and frequency components on an epoch-by-epoch basis from the EEG signals. These extracted parameters can reveal the intrinsic EEG signal changes as well as the underlying stage transitions. To improve the efficacy of feature extraction, an elimination-based feature selection method has been applied on the initial feature vectors. This diminishes redundant and noisy points, providing each patient with a lower dimensional and independent final feature form. In this context, our work is distinguished from that of others currently prevailing. Usually, these latter approaches adopted feature extraction processes which employed time-consuming high-dimensional parameter sets. Machine learning approaches that are considered as state of the art have been employed to build patient-specific binary classifiers that can divide the extracted feature parameters into preictal and interictal groups. Through out-of-sample evaluation on the intracranial EEG (iEEG) recordings provided by the publicly available Freiburg data set, promising prediction performance has been attained. Specifically, we have achieved 98.8% sensitivity results on the 19 patients included in our experiment, where only one of 83 seizures across all patients was not predicted. To make this investigation more comprehensive, we have conducted extensive comparative studies with other recently published competing approaches, in which the advantages of our method are highlighted.
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[Clinical significance of ITP-BAT bleeding grading system for patients with immune thrombocytopenia].
Zhonghua Xue Ye Xue Za Zhi
PUBLISHED: 09-24-2014
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To explore the clinical significance, reliability and responsiveness of ITPBAT bleeding grading system for patients with immune thrombocytopenia (ITP).
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[Clinical characteristics and risk factors for major thrombosis in 604 Chinese patients with low-risk essential thrombocythemia].
Zhonghua Xue Ye Xue Za Zhi
PUBLISHED: 09-24-2014
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To analyze clinical and molecular characteristics of low-risk essential thrombocythemia (ET) in a large cohort of Chinese patients and to explore risk factors for major thrombosis and treatment strategies.
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Lupeol enhances radiosensitivity of human hepatocellular carcinoma cell line SMMC-7721 in vitro and in vivo.
Int. J. Radiat. Biol.
PUBLISHED: 09-23-2014
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Abstract Purpose: The current study investigated the effect of lupeol, a pentacyclictriterpene, on the radiosensitivity of a human hepatocellular carcinoma (HCC) in vitro and in vivo xenografts. Methods: SMMC-7721 cells were exposed to ?-radiation with or without lupeol and assayed for proliferation, clonogenic survival, apoptosis and cell cycle distribution. The cells were also analyzed by western blotting for the expression levels of the proteins involved in apoptosis. Finally radiosensitization by lupeol was assessed in HCC xenograft model. Results: Lupeol further suppressed the proliferation and colonogenic survival of the SMMC-7721 cells exposed to ?-radiation. It could also induce the accumulation of cells in G2/M phase together with ?-radiation. The data also indicated that lupeol sensitized SMMC-7721 cells exposed to ?-radiation to apoptosis and activated the apoptotic proteins including caspase-9 and PARP. Administration of lupeol with radiation in HCC xenograft model produced a significant tumor growth delay compared with radiation or lupeol alone and was well tolerated. Conclusion: Lupeol significantly enhanced the radiosensitivity of SMMC-7721 cells in vitro and in vivo. The mechanisms involved could be cell cycle arrest and induction of apoptosis. Our studies suggest that lupeol has the potential to be developed as an adjuvant for radiotherapy in HCC.
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[Treatment of infantile subglottic hemangioma by microdebrider].
Zhonghua Er Bi Yan Hou Tou Jing Wai Ke Za Zhi
PUBLISHED: 09-23-2014
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To investigate the clinical features, minimally invasive treatment, and outcomes of subglottic hemangioma in infants.
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Effect of Lung Squamous Cell Carcinoma Tumor Microenvironment on the CD105(+) Endothelial Cell Proteome.
J. Proteome Res.
PUBLISHED: 09-19-2014
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In lung cancer, antiangiogenic treatment targeting tumor endothelial cells (ECs) provides a survival advantage. To fully elucidate the behavior of ECs in a tumor microenvironment, high-purity (>98%) normal, paratumor-, and tumor-derived CD105(+) ECs were purified from lung squamous cell carcinoma by incubating cells with anti-CD105 antibody-coated magnetic beads. These cells exhibited typical EC characteristics. Totally, 1765 proteins were identified with high confidence by isobaric stable isotope tags and two-dimensional LC/MS/MS (iTRAQ-2DLC/MS/MS). In particular, 178 and 162 proteins were differentially expressed in paratumor- and tumor-derived ECs, respectively, compared to normal ECs. The up- and down-regulation trends showed good interassay correlation. Using gene ontology, they were classified into genes involved in major reprogramming of cellular metabolic processes, oxidative stress response, redox homeostasis, apoptosis, and platelet degranulation/activation. Moreover, tumor angiogenesis-initiating ECs appeared to acquire distinct properties. For example, cell migration and regulation of smooth muscle cell migration of paratumor-derived ECs were significantly faster than that of normal and tumor-derived ECs. Among them, two migration-associated proteins, neuropilin 1 and platelet-derived growth factor receptor ? predominantly expressed in ECs of paratumor from 16 patients with lung squamous cell carcinoma, were identified as potential biomarkers for antiangiogenic therapy.
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A 12-Bit High-Speed Column-Parallel Two-Step Single-Slope Analog-to-Digital Converter (ADC) for CMOS Image Sensors.
Sensors (Basel)
PUBLISHED: 09-16-2014
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A 12-bit high-speed column-parallel two-step single-slope (SS) analog-to-digital converter (ADC) for CMOS image sensors is proposed. The proposed ADC employs a single ramp voltage and multiple reference voltages, and the conversion is divided into coarse phase and fine phase to improve the conversion rate. An error calibration scheme is proposed to correct errors caused by offsets among the reference voltages. The digital-to-analog converter (DAC) used for the ramp generator is based on the split-capacitor array with an attenuation capacitor. Analysis of the DAC's linearity performance versus capacitor mismatch and parasitic capacitance is presented. A prototype 1024 × 32 Time Delay Integration (TDI) CMOS image sensor with the proposed ADC architecture has been fabricated in a standard 0.18 ?m CMOS process. The proposed ADC has average power consumption of 128 ?W and a conventional rate 6 times higher than the conventional SS ADC. A high-quality image, captured at the line rate of 15.5 k lines/s, shows that the proposed ADC is suitable for high-speed CMOS image sensors.
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Characterization of the microRNA pool and the factors affecting its regulatory potential.
Integr Biol (Camb)
PUBLISHED: 09-16-2014
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The regulation of gene expression by microRNAs (miRNAs) is complex due to a number of variables involved. The potential for one miRNA to target many genes, the presence of multiple miRNA response elements (MREs) in one mRNA molecule and the interplay between RNAs that share common MREs each add a layer of complexity to the process; making it difficult to determine how regulation of gene expression by miRNAs works within the context of the system as a whole. In this study, we used luciferase report vectors inserted with different 3'UTR fragments as probes to detect the repressive effect of the miRNA pool on gene expression and uncovered some essential characteristics of gene regulation mediated by the miRNA pool, such as the nonlinear correlative relationship between the regulatory potential of a miRNA pool and the number of potential MREs, the buffering effect and the saturating effect of the miRNA pool, and the restrictive effect caused by the density of MREs. Through expressing gradient concentration of 3'UTR fragments, we indirectly detected the regulatory potential of the competing endogenous RNA (ceRNA) pool and analysed its effect on the regulatory potential of the miRNA pool. Our results provide some new insights into miRNA pool mediated gene regulation.
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[Molecular characteristics and antibiotic resistance of Vibrio cholerae O139 in Shandong province].
Zhonghua Yu Fang Yi Xue Za Zhi
PUBLISHED: 09-16-2014
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To investigate the molecular epidemiological characteristics and antibiotic resistance profiles of Vibrio cholerae O139 in Shandong province.
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One-pot synthesis of protein-embedded metal-organic frameworks with enhanced biological activities.
Nano Lett.
PUBLISHED: 09-15-2014
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Protein molecules were directly embedded in metal-organic frameworks (MOFs) by a coprecipitation method. The protein molecules majorly embedded on the surface region of MOFs display high biological activities. As a demonstration of the power of such materials, the resulting Cyt c embedded in ZIF-8 showed a 10-fold increase in peroxidase activity compared to free Cyt c in solution and thus gave convenient, fast, and highly sensitive detection of trace amounts of explosive organic peroxides in solution.
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Ink-jet printing an optimal multi-enzyme system.
Chem. Commun. (Camb.)
PUBLISHED: 09-13-2014
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A method using ink-jet printing for constructing multi-enzyme systems was proposed, in which a precisely defined enzyme ratio and two-dimensional distribution was obtained by the preset 'color' values. The applications of the print-on-paper multi-enzyme systems were exemplified by the detection of glucose and the design of an enzyme-enabled two-dimensional code.
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[Quantitative evaluation of three-dimensional reconstruction accuracy of edentulous models in centric relation].
Zhonghua Kou Qiang Yi Xue Za Zhi
PUBLISHED: 09-10-2014
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To reconstruct the centric relation of upper and lower edentulous dental models with two methods, to compare the reconstruction accuracy of the vertical and horizontal distance, and to provide some references to computer aided design and computer aided manufacture for complete denture.
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Identification of Klebsiella pneumoniae strains harboring inactive extended-spectrum beta-lactamase antibiotic-resistance genes.
Chin. Med. J.
PUBLISHED: 09-06-2014
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The extended-spectrum beta-lactamase (ESBL)-producing Klebsiella pneumoniae has increasingly become a major contributor to nosocomial infections and can exhibit multiple antibiotic resistance. Previous studies have focused on the resistance genes in ESBL-producing strains, and the resistance-associated genetic environment of non-ESBL-producing strains has been ignored until now. Here, we investigated the occurrence and characteristics of non-ESBL-producing K. pneumoniae, which potentially carries unexpressed resistance genes.
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Ovarian preservation in young patients with stage I cervical adenocarcinoma: a surveillance, epidemiology, and end results study.
Int. J. Gynecol. Cancer
PUBLISHED: 09-05-2014
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Although a large part of patients with cervical adenocarcinoma are young, oophorectomy is commonly performed in those who receive hysterectomy for fear of ovarian metastasis. The purpose of this study was to examine the safety of ovarian preservation in young women with cervical adenocarcinoma.
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Emerging medical informatics research trends detection based on MeSH terms.
Inform Health Soc Care
PUBLISHED: 09-04-2014
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The aim of this study is to analyze the research trends of medical informatics over the last 12 years. A new method based on MeSH terms was proposed to identify emerging topics and trends of medical informatics research. Informetric methods and visualization technologies were applied to investigate research trends of medical informatics. The metric of perspective factor (PF) embedding MeSH terms was appropriately employed to assess the perspective quality for journals. The emerging MeSH terms have changed dramatically over the last 12 years, identifying two stages of medical informatics: the "medical imaging stage" and the "medical informatics stage". The focus of medical informatics has shifted from acquisition and storage of healthcare data by integrating computational, informational, cognitive and organizational sciences to semantic analysis for problem solving and clinical decision-making. About 30 core journals were determined by Bradford's Law in the last 3 years in this area. These journals, with high PF values, have relative high perspective quality and lead the trend of medical informatics.
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RGS2 suppresses breast cancer cell growth via a MCPIP1-dependent pathway.
J. Cell. Biochem.
PUBLISHED: 09-04-2014
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Regulator of G protein signaling 2 (RGS2) is a member of a family of proteins that functions as a GTPase-activating protein (GAP) for G? subunits. RGS2 mRNA expression is lower in breast cancerous tissues than in normal tissues. In addition, expression of RGS2 is also lower in MCF7 (cancerous breast cells) than in MCF10A (normal breast cells). Here we investigated whether RGS2 inhibits growth of breast cancer cells. RGS2 overexpression in MCF7 cells inhibited epidermal growth factor-or serum-induced proliferation. In HEK293T cells expressing RGS2, cell growth was also significantly suppressed (In addition, exogenous express ion of RGS2 in HEK293T cells resulted in the significant suppression of cell growth). These results suggest that RGS2 may have a tumor suppressor function. MG-132 treatment of MCF7 cells increased endogenous or exogenous RGS2 levels, suggesting a post-transcriptional regulatory mechanism that controls RGS2 protein levels. RGS2 protein was degraded polyubiquitinated the K71 residue, but stabilized by deubiquitinase monocyte chemotactic protein-induced protein 1 (MCPIP1), and not affected by dominant negative mutant (C157A) of MCPIP1. Gene expression profiling study showed that overexpression of RGS2 decreased levels of testis specific Y encoded like protein 5 (TSPYL5), which plays a causal role in breast oncogenesis. TSPYL5 protein expression was low in MCF10A and high in MCF7 cells, showing the opposite aspect to RGS2 expression. Additionally, RGS2 or MCPIP1 overexpression in MCF7 cells decreased TSPYL5 protein level, indicating that RGS2 stabilized by MCPIP1 have diminished TSPYL5 protein levels , thereby exerting an inhibitory effect of breast cancer cell growth. J. Cell. Biochem. © 2014 Wiley Periodicals, Inc.
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[Research of fenvalerate induce hippocampal neurons injury through interfering with estrogen action].
Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi
PUBLISHED: 09-04-2014
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To investigate whether fenvalerate can induce mouse hippocampal nerve cell damage by interfering with estrogen (E2) effect.
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[Research of fenvalerate induced neurodevelopmental toxicity by interfering with the action of estrogen].
Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi
PUBLISHED: 09-04-2014
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To investigate the estrogen interference property of fenvalerate in neurodevelopmental toxicity.
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Success With Single-Agent Immunosuppression for Multifocal Choroidopathies.
Am. J. Ophthalmol.
PUBLISHED: 09-03-2014
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To evaluate the success of single-agent immunosuppression for patients with the posterior uveitides, birdshot chorioretinitis, multifocal choroiditis with panuveitis, and punctate inner choroiditis.
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miR181c promotes apoptosis and suppresses proliferation of metanephric mesenchyme cells by targeting Six2 in vitro.
Cell Biochem. Funct.
PUBLISHED: 09-03-2014
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Increasingly recognized importance has been assumed for microRNA (miRNA) in the regulation of the delicate balance of gene expression. In our study, we aimed to explore the regulation role of miR181c towards Six2 in metanephric mesenchyme (MM) cells. Bioinformatics analysis, luciferase assay and semi-quantitative real-time (RT) PCR, subsequently RT PCR, Western blotting, 5-ethynyl-2'-deoxyuridine cell proliferation assay, Cell Counting Kit-8 assay, immunofluorescence and flow cytometry, were employed to verify the modulation function of miR181c on Six2 in the mK3 MM cell line that is one kind of MM cells. miR181c was predicted to bind the 3' untranslated region of Six2 by bioinformatics analysis, which was subsequently validated by the in vitro luciferase reporter assay. Moreover, transfection of miR181c mimic can decrease the expression of Six2 both in mRNA and protein levels in mK3 cells. Still, ectopic expression of miR181c inhibits the proliferation, promotes the apoptosis and even makes the nephron progenitor phenotype lose mK3 cells. These results revealed the ability of a single miRNA-miR181c to downregulate the expression of Six2, restrain the proliferation and promote the apoptosis that even makes the nephron progenitor phenotype lose MM cells, suggesting a potential role of miR181c during the kidney development. Copyright © 2014 John Wiley & Sons, Ltd.
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NF-?B affects proliferation and invasiveness of breast cancer cells by regulating CD44 expression.
PLoS ONE
PUBLISHED: 09-03-2014
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NF-?B plays an important role in cancer initiation and progression. CD44, a cell surface glycoprotein, is involved in many cellular processes including cell adhesion, migration and proliferation. However, whether and how the two molecules interact in breast cancer is not clear. In recent years, the up-regulation of CD44 has served as a marker for tumor initiating cells in breast cancer and other cancer types. Despite the important role of CD44 in cellular processes and cancer, the mechanism underlying CD44 up-regulation in cancers remains poorly understood. Previously, we have identified a novel cis-element, CR1, located upstream of the CD44 promoter. We demonstrated that NF-?B and AP-1 are key trans-acting factors that interact with CR1. Here, we further analyzed the role of NF-?B in regulating CD44 expression in triple negative breast cancer cells, MDA-MB-231 and SUM159. Inhibition of NF-?B by Bay-11-7082 resulted in a reduction in CD44 expression. CD44 repression via NF-?B inhibition consequently decreased proliferation and invasiveness of breast cancer cells. These findings provide not only new insight into the molecular mechanism underlying CD44 regulation but also potential therapeutic targets that may help eliminate chemo- and radiation-resistant cancer cells.
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[A twin study in Qingdao and Lishui: heritability of exercise participation and sedentary behavior].
Zhonghua Liu Xing Bing Xue Za Zhi
PUBLISHED: 09-02-2014
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To investigate both genetic and environmental influences on the exercise participation and sedentary behavior in Qingdao, Shandong province and 326 in Lishui, Zhejiang province.
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Composition-dependent photoluminescence intensity and prolonged recombination lifetime of perovskite CH3NH3PbBr(3-x)Cl(x) films.
Chem. Commun. (Camb.)
PUBLISHED: 08-22-2014
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Mixed halide perovskites CH3NH3PbBr3-xClx (x = 0.6-1.2) with different compositions of halogens exhibit drastically changed optical properties. In particular, the thin films prepared with these perovskites demonstrate extraordinary photoluminescence emission intensities and prolonged recombination lifetimes up to 446 ns, which are desirable for light emitting and photovoltaic applications.
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[Analysis of erythroid-specific blood group genes using un-mobilized peripheral stem cells cultured in vitro].
Zhonghua Yi Xue Yi Chuan Xue Za Zhi
PUBLISHED: 08-15-2014
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To analyze specific expression of blood group genes using nucleated erythroid cells cultured from un-mobilized peripheral stem cells in vitro.
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[Establishment and application of quantitative detection for heteroplasmic mtDNA mutation 3243A?G].
Zhonghua Yi Xue Yi Chuan Xue Za Zhi
PUBLISHED: 08-15-2014
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To develop a rapid, simple, cost-effective, accurate and sensitive method for quantitative detection of mitochondrial DNA (mtDNA) 3243A?G mutation in order to provide reference for selecting the best detection method under different conditions.
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Multi-atlas segmentation with particle-based group-wise image registration.
Proc Soc Photo Opt Instrum Eng
PUBLISHED: 07-31-2014
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We propose a novel multi-atlas segmentation method that employs a group-wise image registration method for the brain segmentation on rodent magnetic resonance (MR) images. The core element of the proposed segmentation is the use of a particle-guided image registration method that extends the concept of particle correspondence into the volumetric image domain. The registration method performs a group-wise image registration that simultaneously registers a set of images toward the space defined by the average of particles. The particle-guided image registration method is robust with low signal-to-noise ratio images as well as differing sizes and shapes observed in the developing rodent brain. Also, the use of an implicit common reference frame can prevent potential bias induced by the use of a single template in the segmentation process. We show that the use of a particle guided-image registration method can be naturally extended to a novel multi-atlas segmentation method and improves the registration method to explicitly use the provided template labels as an additional constraint. In the experiment, we show that our segmentation algorithm provides more accuracy with multi-atlas label fusion and stability against pair-wise image registration. The comparison with previous group-wise registration method is provided as well.
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[Impact factors on physical activity level among the urban junior high school personnel in Hangzhou].
Zhonghua Liu Xing Bing Xue Za Zhi
PUBLISHED: 07-26-2014
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To explore the level of physical activities (PA) and related influencing factors among junior high school personnel in Hangzhou.
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[Relationship between socioeconomic status and the prevalence of cardiovascular disease among retired residents living in a community, Shanghai].
Zhonghua Liu Xing Bing Xue Za Zhi
PUBLISHED: 07-26-2014
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To explore the relationship between socioeconomic status and the risk factors of cardiovascular diseases in retirees from a community in Shanghai.
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Stimulation of gene transfection by silicon nanowire arrays modified with polyethylenimine.
ACS Appl Mater Interfaces
PUBLISHED: 07-23-2014
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In this work, a novel gene delivery strategy was proposed based on silicon nanowire arrays modified with high-molecular-weight 25 kDa branched polyethylenimine (SN-PEI). Both the plasmid DNA (pDNA) binding capacity and the in vitro gene transfection efficiency of silicon nanowire arrays (SiNWAs) were significantly enhanced after modification with high-molecular-weight bPEI. Moreover, the transfection efficiency was substantially further increased by the introduction of free pDNA/PEI complexes formed by low-molecular-weight branched PEI (bPEI, 2 kDa). Additionally, factors affecting the in vitro transfection efficiency of the novel gene delivery system were investigated in detail, and the transfection efficiency was optimized on SN-PEI with a bPEI grafting time of 3 h, an incubation time of 10 min for tethered pDNA/PEI complexes consisting of high-molecular-weight bPEI grafted onto SiNWAs, and with an N/P ratio of 80 for free pDNA/PEI complexes made of low-molecular-weight bPEI. Together, our results indicate that high-molecular-weight bPEI modified SiNWAs can serve as an efficient platform for gene delivery.
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[High risk factors of the third level of lymphatic metastasis in breast cancer patients received radical/modified mastectomy: an analysis of 746 cases].
Zhonghua Wai Ke Za Zhi
PUBLISHED: 07-19-2014
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To study the high risk factors of the third level of lymphatic metastasis in breast cancer patients to guide clinical practice.
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Stable and Low-Cost Mesoscopic CH3 NH3 PbI2 Br Perovskite Solar Cells by using a Thin Poly(3-hexylthiophene) Layer as a Hole Transporter.
Chemistry
PUBLISHED: 07-15-2014
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Mesoscopic perovskite solar cells using stable CH3 NH3 PbI2 Br as a light absorber and low-cost poly(3-hexylthiophene) (P3HT) as hole-transporting layer were fabricated, and a power conversion efficiency of 6.64?% was achieved. The partial substitution of iodine with bromine in the perovskite led to remarkably prolonged charge carrier lifetime. Meanwhile, the replacement of conventional thick spiro-MeOTAD layer with a thin P3HT layer has significantly reduced the fabrication cost. The solar cells retained their photovoltaic performance well when they were exposed to air without any encapsulation, presenting a favorable stability. The combination of CH3 NH3 PbI2 Br and P3HT may render a practical and cost-effective solid-state photovoltaic system. The superior stability of CH3 NH3 PbI2 Br is also promising for other photoconversion applications.
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[A survey on the sodium content of customers' orderings at three restaurants in Beijing].
Zhonghua Liu Xing Bing Xue Za Zhi
PUBLISHED: 07-11-2014
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To survey the mean sodium content of menu offering and customers' orderings on sodium when eating out.
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Identification of up- and down-regulated proteins in doxorubicin-resistant uterine cancer cells: Reticulocalbin-1 plays a key role in the development of doxorubicin-associated resistance.
Pharmacol. Res.
PUBLISHED: 07-09-2014
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Drug resistance is a frequent cause of failure in cancer chemotherapy treatments. In this study, a pair of uterine sarcoma cancer lines, MES-SA, and doxorubicin-resistant partners, MES-SA/DxR-2?M cells and MES-SA/DxR-8?M cells, as a model system to investigate resistance-dependent proteome alterations and to identify potential therapeutic targets. We used two-dimensional differential gel electrophoresis (2D-DIGE) and matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) to perform this research and the results revealed that doxorubicin-resistance altered the expression of 208 proteins in which 129 identified proteins showed dose-dependent manners in response to the levels of resistance. Further studies have used RNA interference, H2A.X phosphorylation assay, cell viability analysis, and analysis of apoptosis against reticulocalbin-1 (RCN1) proteins, to prove its potency on the formation of doxorubicin resistance as well as the attenuation of doxorubicin-associated DNA double strand breakage. To sum up, our results provide useful diagnostic markers and therapeutic candidates such as RCN1 for the treatment of doxorubicin-resistant uterine cancer.
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[Analysis of effect on infectious diseases outbreak detection performance by classifying provinces for moving percentile method].
Zhonghua Yu Fang Yi Xue Za Zhi
PUBLISHED: 06-28-2014
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Providing evidences for further modification of China Infectious Diseases Automated-alert and Response System (CIDARS) via analyzing the outbreak detection performance of Moving Percentile Method (MPM) by optimizing thresholds in different provinces.
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Elevated circulating levels of CTRP1, a novel adipokine, in diabetic patients.
Endocr. J.
PUBLISHED: 06-24-2014
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Complement C1q tumor necrosis factor-related protein 1 (CTRP1), an adipose tissue-derived adipokine has been shown to decrease blood glucose levels and to improve metabolism of glucose in mice. In addition, CTRP1 has exhibited significant association with BMI, adiponectin and TNF-? in diabetic animal models. However, there are no published studies addressing CTRP1 levels in type 2 diabetic patients. Therefore, it was of interest to evaluate plasma CTRP1 levels and associated clinical parameters and biomarkers in patients with type 2 diabetes. 135 subjects were recruited to this study, including 62 type 2 diabetic patients (DM group) and 73 healthy subjects (control group). We measured biochemical parameters, CTRP1, TNF-? and adiponectin using enzyme-linked immunosorbent assay (ELISA). Plasma CTRP1 levels showed a significant difference between the DM group and the control group (646.3 ± 154.4 ng/mL vs. 442.6 ± 165.4 ng/mL, p < 0.01). In addition, CTRP1 was strongly positively associated with BMI, glucose levels, HbA1c, HOMA-IR and TNF-? in diabetic patients. CTRP1 showed negative correlation with adiponectin. In Multivariate regression analysis, CTRP1 was strongly independently associated with diabetes when CTRP1 levels were analyzed by both as a continuous variable and quartile (OR: 1.009, 95% CI: 1.004-1.015, p < 0.05; OR: 2.443, 95% CI: 1.379-4.182, p < 0.01, respectively). Increased plasma CTRP1 was independently associated with type 2 diabetes. Profiling of plasma adipokines such as CTRP1 is particularly important to obtain a greater understanding of their contribution to the type 2 diabetic state.
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[Development of a method for the separation of HLA-A, -B and -C haploid using biotinylated probe and streptavidin magnetic beads].
Zhonghua Yi Xue Yi Chuan Xue Za Zhi
PUBLISHED: 06-15-2014
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To develop a method for separating the human leukocyte antigen (HLA)-A, -B and -C haploid using biotinylated probes and streptavidin magnetic beads in order to solve ambiguous HLA genotyping results.
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A fluorescence resonance energy transfer (FRET) biosensor based on graphene quantum dots (GQDs) and gold nanoparticles (AuNPs) for the detection of mecA gene sequence of Staphylococcus aureus.
Biosens Bioelectron
PUBLISHED: 06-06-2014
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In this work, a novel fluorescence resonance energy transfer (FRET) biosensor based on graphene quantum dots (GQDs) and gold nanoparticles (AuNPs) pairs was developed for Staphylococcus aureus specific gene sequence detection. This FRET biosensor platform was realized by immobilization of capture probes on GQDs and conjugation of reporter probes on AuNPs. Target oligos then co-hybridized with capture probes and reporter probes to form a sandwich structure which brought GQDs and AuNPs to close proximity to trigger FRET effect. The fluorescence signals before and after addition of targets were measured and the fluorescence quenching efficiency could reach around 87% with 100nM target oligo. The limit of detection (LOD) of this FRET biosensor was around 1nM for S.aureus gene detection. Experiments with both single-base mismatched oligos and double-base mismatched oligos demonstrated the good sequence selectivity of this FRET biosensor.
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The clinical presentation and electrophysiological findings of porphyric neuropathies: a follow-up study.
Muscle Nerve
PUBLISHED: 06-04-2014
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Introduction: A case series of acute intermittent porphyria (AIP) is described which focuses on the clinical course of the disease with regard to neurological manifestations of the peripheral nervous system. Methods: Eight patients were diagnosed with AIP on the basis of characteristic clinical findings, erythrocyte porphobilinogendeaminase activity, neuropathic patterns, serial changes in nerve conduction studies (NCS), and temporal relationship of central nervous system involvement. Results: Six patients diagnosed with AIP less than 2 months after symptom onset had neuropathy that was predominantly upper extremity, motor, and proximal. NCS recovery rates were slower in the lower than the upper limbs. Two patients diagnosed more than 2 months after symptom onset had distal sensorimotor polyneuropathy. Discussion: The findings of this case series suggest that the peripheral nerves may be differentially and selectively involved in different diagnostic stages of porphyric neuropathy. © 2014 Wiley Periodicals, Inc.
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Overexpression of SIRT1 is a poor prognostic factor for advanced colorectal cancer.
Chin. Med. J.
PUBLISHED: 06-04-2014
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Sirtuin 1 (SIRT1) has been reported to have diverse roles in various biological processes through deacetylation of histone and nonhistone proteins. However, the correlations among SIRT1 protein expression, clinicopathological parameters, and survival of colorectal cancer patients remain unclear.
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Design and synthesis of Lapatinib derivatives containing a branched side chain as HER1/HER2 targeting antitumor drug candidates.
Eur J Med Chem
PUBLISHED: 06-01-2014
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A series of Lapatinib derivatives were designed and prepared by changing the straight alkyl side chain of Lapatinib into a branched one. ELISA assay and western blot analysis showed that these derivatives can significantly inhibit HER1/HER2 as well as their downstream signal transduction proteins. In vitro cytotoxicity assay revealed that these compounds had potent cytotoxic effect against the HER1/HER2-overexpressing cancer cells. A representive compound, 2i, showed potent in vivo antitumor activity comparable to Lapatinib, which was found to block the cell-cycle progression of BT474 cells in the G1 phase causing tumor cell apoptosis in the flow cytometry study. Moreover, the pharmacokinetic investigation on 2i also indicated it had a good performance on both absorption and elimination profiles.
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What is Visualize?

JoVE Visualize is a tool created to match the last 5 years of PubMed publications to methods in JoVE's video library.

How does it work?

We use abstracts found on PubMed and match them to JoVE videos to create a list of 10 to 30 related methods videos.

Video X seems to be unrelated to Abstract Y...

In developing our video relationships, we compare around 5 million PubMed articles to our library of over 4,500 methods videos. In some cases the language used in the PubMed abstracts makes matching that content to a JoVE video difficult. In other cases, there happens not to be any content in our video library that is relevant to the topic of a given abstract. In these cases, our algorithms are trying their best to display videos with relevant content, which can sometimes result in matched videos with only a slight relation.