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Find video protocols related to scientific articles indexed in Pubmed.
Simultaneous determination of trifolirhizin, (-)-maackiain, (-)-sophoranone, and 2-(2,4-dihydroxyphenyl)-5,6-methylenedioxybenzofuran from Sophora tonkinensis in rat plasma by liquid chromatography with tandem mass spectrometry and its application to a pharmacokinetic s
J Sep Sci
PUBLISHED: 09-30-2014
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A new liquid chromatography with tandem mass spectrometry method was developed and validated for the simultaneous determination of trifolirhizin, (-)-maackiain, (-)-sophoranone, and 2-(2,4-dihydroxyphenyl)-5,6-methylenedioxybenzofuran from Sophora tonkinensis in rat plasma using chlorpropamide as an internal standard. Plasma samples (50 ?L) were prepared using a simple deproteinization procedure with 150 ?L of acetonitrile containing 100 ng/mL of chlorpropamide. Chromatographic separation was carried out on an Acclaim RSLC120 C18 column (2.1 × 100 mm, 2.2 ?m) using a gradient elution consisting of 7.5 mM ammonium acetate and acetonitrile containing 0.1% formic acid (0.4 mL/min flow rate, 7.0 min total run time). The detection and quantitation of all analytes were performed in selected reaction monitoring mode under both positive and negative electrospray ionization. This assay was linear over concentration ranges of 50-5000 ng/mL (trifolirhizin), 25-2500 ng/mL ((-)-maackiain), 5-250 ng/mL ((-)-sophoranone), and 1-250 ng/mL 2-(2,4-dihydroxyphenyl)-5,6-methylenedioxybenzofuran) with a lower limit of quantification of 50, 25, 5, and 1 ng/mL for trifolirhizin, (-)-maackiain, (-)-sophoranone, and 2-(2,4-dihydroxyphenyl)-5,6-methylenedioxybenzofuran, respectively. All the validation data, including the specificity, precision, accuracy, recovery, and stability conformed to the acceptance requirements. The results indicated that the developed method is sufficiently reliable for the pharmacokinetic study of the analytes following oral administration of Sophora tonkinensis extract in rats.
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Retear rate in the late postoperative period after arthroscopic rotator cuff repair.
Am J Sports Med
PUBLISHED: 09-03-2014
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Few clinical studies have evaluated the integrity of repaired tendons and identified the timing of retears through the use of serial imaging.
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Risk of Lymph Node Metastasis in Differentiated Type Mucosal Early Gastric Cancer Mixed with Minor Undifferentiated Type Histology.
Ann. Surg. Oncol.
PUBLISHED: 08-14-2014
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The possibility of lymph node metastasis is critical to the assessment of the indication for endoscopic submucosal dissection. The differentiation of tumors is an important predicting factor for lymph node metastasis. Even though gastric cancers frequently show intratumoral heterogeneity, most studies have not considered the effects of the minor histologic components. The purpose of this study was to investigate the relationship between the presence of undifferentiated type histology (UD-min) within differentiated type tumors and lymph node metastases in early gastric cancer confined to the mucosal layer.
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Stimulation of innate immune cells by light-activated TLR7/8 agonists.
J. Am. Chem. Soc.
PUBLISHED: 07-28-2014
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The innate immune response is controlled, in part, by the synergistic interaction of multiple Toll-like receptors (TLRs). This multi-receptor cooperation is responsible for the potent activity of many vaccines, but few tools have been developed to understand the spatio-temporal elements of TLR synergies. In this Communication, we present photo-controlled agonists of TLR7/8. By strategically protecting the active agonist moiety based on an agonist-bound crystal structure, TLR activity is suppressed and then regained upon exposure to light. We confirmed NF-?B production upon light exposure in a model macrophage cell line. Primary cell activity was confirmed by examining cytokine and cell surface marker production in bone-marrow-derived dendritic cells. Finally, we used light to activate dendritic cell sub-populations within a larger population.
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A New Direction of Cancer Classification: Positive Effect of Low-Ranking MicroRNAs.
Osong Public Health Res Perspect
PUBLISHED: 07-20-2014
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Many studies based on microRNA (miRNA) expression profiles showed a new aspect of cancer classification. Because one characteristic of miRNA expression data is the high dimensionality, feature selection methods have been used to facilitate dimensionality reduction. The feature selection methods have one shortcoming thus far: they just consider the problem of where feature to class is 1:1 or n:1. However, because one miRNA may influence more than one type of cancer, human miRNA is considered to be ranked low in traditional feature selection methods and are removed most of the time. In view of the limitation of the miRNA number, low-ranking miRNAs are also important to cancer classification.
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Clinical practice guidelines for gastric cancer in Korea: an evidence-based approach.
J Gastric Cancer
PUBLISHED: 06-26-2014
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Although gastric cancer is quite common in Korea, the treatment outcome is relatively favorable compared to those in western countries. However, there are currently no Korean multidisciplinary guidelines for gastric cancer. Experts from related societies developed guidelines de novo to meet Korean circumstances and requirements, including 23 recommendation statements for diagnosis (n=9) and treatment (n=14) based on relevant key questions. The quality of the evidence was rated according to the GRADE evidence evaluation framework: the evidence levels were based on a systematic review of the literature, and the recommendation grades were classified as either strong or weak. The applicability of the guidelines was considered to meet patients' view and preferences in the context of Korea. The topics of the guidelines cover diagnostic modalities (endoscopy, endoscopic ultrasound, and radiologic diagnosis), treatment modalities (surgery, therapeutic endoscopy, chemotherapy, and radiotherapy), and pathologic evaluation. An external review of the guidelines was conducted during the finalization phase.
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A novel approach for predicting disordered regions in a protein sequence.
Osong Public Health Res Perspect
PUBLISHED: 06-19-2014
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A number of published predictors are based on various algorithms and disordered protein sequence properties. Although many predictors have been published, the study of protein disordered region prediction is ongoing because different prediction methods can find different disordered regions in a protein sequence.
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Determination of toxic heavy metals and speciation of arsenic in seaweeds from South Korea.
Food Chem
PUBLISHED: 05-05-2014
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This study aimed at determining the levels of toxic heavy metals including As, Pb, Cd, Al, Hg and As species, such as, As-III, As-V, MMA, DMA, AsB, and AsC in various edible species of seaweeds from South Korea. ICP-MS was used for determination of As, Pb and Cd, ICP-OES was used for Al, DMA was used for Hg, and LC-ICP-MS was used for As speciation. The analytical methods were validated by linearity, detection limits, precision, accuracy and recovery experiments, obtaining satisfactory results in all cases. From the results toxic heavy metals were found in the decreasing order of: Al>As>Pb-Cd>Hg. Generally concentrations of all analysed heavy metals and both organic and inorganic species of As were very low compared to PTWIs specified by JECFA and EC. Their contribution to the overall intake by the subject seafoods was found very low and thus would not pose any threat to consumers.
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Directing the immune system with chemical compounds.
ACS Chem. Biol.
PUBLISHED: 04-30-2014
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Agonists of immune cell receptors direct innate and adaptive immunity. These agonists range in size and complexity from small molecules to large macromolecules. Here, agonists of a class of immune cell receptors known as the Toll-like receptors (TLRs) are highlighted focusing on the distinctive molecular moieties that pertain to receptor binding and activation. How the structure and combined chemical signals translate into a variety of immune responses remain major questions in the field. In this structure-focused review, we outline potential areas where the tools of chemical biology could help decipher the emerging molecular codes that direct immune stimulation.
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An analysis of normative data on the knee rotatory profile and the usefulness of the Rotatometer, a new instrument for measuring tibiofemoral rotation: the reliability of the knee Rotatometer.
Knee Surg Sports Traumatol Arthrosc
PUBLISHED: 04-23-2014
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This study proposes a simple and noninvasive instrument called the "Rotatometer" to measure tibiofemoral rotation and investigates its clinical applicability to the assessment of static rotational knee laxity.
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4-1BB-based isolation and expansion of CD8+ T cells specific for self-tumor and non-self-tumor antigens for adoptive T-cell therapy.
J. Immunother.
PUBLISHED: 04-10-2014
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Adoptive T-cell therapy is a promising approach to the immunotherapy of cancer, but for it to be a general cancer therapy a simple and standardized procedure for producing tumor-specific CD8 T cells is needed. On the basis of a unique property of 4-1BB (CD137), the selective expression on activated T cells, we have developed a simple and practical protocol to produce antigen-specific CD8 T cells from peripheral blood mononuclear cells. We have proved the feasibility of this procedure by isolating and expanding cytomegalovirus-specific CD8 T cells, and applied the procedure to produce Epstein-Barr virus (EBV)-specific CD8 T cells. By using this procedure, we could readily produce 10-10 antigen-specific CD8 T cells from 30 to 50 mL of blood in about 4 weeks. Moreover, our protocol allowed us to produce, from solid cancer patients, CD8 T cells that were specific for self/tumor antigens such as human telomerase reverse transcriptase (hTERT). It is interesting to note that, we were unable to amplify hTERT-specific CD8 T cells from healthy donors. Our protocol can be readily translated into cGMP-compliant production and is currently being used to produce EBV-specific CD8 T cells for phase I clinical trial. We believe that our method will provide a practical and effective option for adoptive T-cell therapy in the clinic.
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Long-term outcome comparison of endoscopic resection and surgery in early gastric cancer meeting the absolute indication for endoscopic resection.
Gastrointest. Endosc.
PUBLISHED: 03-12-2014
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Endoscopic resection (ER) of early gastric cancer (EGC) meeting the absolute indication has excellent long-term outcomes.
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The association between Helicobacter pylori status and incidence of metachronous gastric cancer after endoscopic resection of early gastric cancer.
Helicobacter
PUBLISHED: 03-10-2014
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The long-term effect of Helicobacter pylori eradication in preventing metachronous gastric cancer (GC) development after endoscopic resection (ER) of early gastric cancer (EGC) remains controversial. The aim of this study was to investigate the effect of H. pylori status on the incidence of metachronous GC after ER during long-term follow-up.
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Inhibitory effects of SKI3246, the rhizome extract of Atractylodes japonica, on visceral hypersensitivity in experimental irritable bowel syndrome rat models.
Arch. Pharm. Res.
PUBLISHED: 03-07-2014
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We evaluated the effect of SKI3246, the 50 % ethanol extract of the rhizome of Atractylodes japonica, on visceral hypersensitivity, which is a major characteristic feature of IBS. We used various rat models of visceral hypersensitivity to assess the visceral pain responses to colorectal distension (CRD) in comparison with conventional IBS treatments. Oral administration of SKI3246 dose-dependently and significantly attenuated the abdominal withdrawal reflex (AWR) score in a model of acetic acid-induced visceral hypersensitivity. We also found that it reduced the number of abdominal contractions in response to CRD in a model of 2,4,6-trinitrobenzenesulfonic acid-induced visceral hypersensitivity, which was comparable to ramosetron or alosetron. Furthermore, treatment with SKI3246 also increased the pain threshold and abolished the elevated AWR scores to CRD in a rat model of neonatal maternal separation. We presumed that the modulation of the NK2 receptor is involved in the inhibitory activity of SKI3246 on the basis that it significantly inhibited the contraction of the distal colonic muscle induced by neurokinin A, the NK2 receptor agonist. The present results indicate that SKI3246 has the potential to be an effective therapeutic agent for IBS, especially insofar as it can relieve visceral hypersensitivity.
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Characteristics of gastric cancer according to Helicobacter pylori infection status.
J. Gastroenterol. Hepatol.
PUBLISHED: 03-06-2014
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The definition and incidence of gastric cancer (GC) without Helicobacter pylori (Hp) infection varies between studies. The aim of our study was to compare the characteristics of GC according to Hp infection status.
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Intravenous iron supplementation may be superior to observation in acute isovolemic anemia after gastrectomy for cancer.
World J. Gastroenterol.
PUBLISHED: 03-04-2014
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To determine whether the application of post-operative intravenous (IV)-iron for acute isovolemic anemia after gastrectomy for cancer may be effective.
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[Synopsis on clinical practice guideline of gastric cancer in Korea: an evidence-based approach].
Korean J Gastroenterol
PUBLISHED: 02-25-2014
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Although, gastric cancer is quite common in Korea, the treatment outcome is relatively favorable compared to that of Western countries. However, there is no Korean multidisciplinary guideline for gastric cancer and thus, a guideline adequate for domestic circumstances is required. Experts from related societies developed 22 recommendation statements for the diagnosis (n=9) and treatment (n=13) based on relevant key questions. Evidence levels based on systematic review of literatures were classified as five levels from A to E, and recommendation grades were classified as either strong or weak. The topics of this guideline cover diagnostic modalities (endoscopy, endoscopic ultrasound, radiologic diagnosis), treatment modalities (surgery, therapeutic endoscopy, chemotherapy, radiotherapy) and pathologic evaluation. External review of the guideline was conducted at the finalization phase.
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Peroxisome proliferator-activated receptor ? upregulates galectin-9 and predicts prognosis in intestinal-type gastric cancer.
Int. J. Cancer
PUBLISHED: 02-18-2014
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The importance of PPAR? (peroxisome proliferator-activated receptor ?) in gastric cancer (GC) is unclear. We investigated the role of PPAR? in GC cell lines and an animal model, and its prognostic significance of PPAR? in GC patients. We controlled PPAR? and galectin-9 expression by using siRNAs and lentiviral constructs. Interaction between PPAR? and galectin-9 was evaluated using luciferase and chromatin immunoprecipitation assays. PPAR? expression in GCs was determined by immunohistochemical staining of tissue microarrays and survival analysis was done. Overexpression of PPAR? was accompanied by increased galectin-9. Enhanced PPAR? or galectin-9 expression increased E-cadherin expression; decreased expression of N-cadherin, fibronectin, snail, twist and slug and reduced cell invasion and migration. PPAR? bound to the galectin-9 promoter region. Galectin-9 activity increased in PPAR?-overexpressing cells but decreased in PPAR? siRNA-treated cells. In a zebrafish xenograft model, the number of migrated cancer cells and number of fish with AGS cells in the tail vein were reduced in PPAR?-overexpressing GC cells. PPAR? was expressed in 462 of the 688 patients (69.2%) with GC. In 306 patients with intestinal-type GC, those with PPAR?-positive tumors had lower overall and cancer-specific mortalities than those with PPAR?-negative tumors. PPAR? expression was an independent prognostic factor for overall and GC-specific mortality in patients with intestinal-type GC (adjusted hazard ratio, 0.42; 95% CI, 0.22-0.81). PPAR? inhibits cell invasion, migration and epithelial-mesenchymal transition through upregulation of galectin-9 in vitro and in vivo.
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Three-dimensional conformal coatings through the entrapment of polymer membrane precursors.
ACS Appl Mater Interfaces
PUBLISHED: 02-03-2014
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We report a technique to coat polymers onto 3D surfaces distinct from traditional spray, spin, or dip coating. In our technique, the surface of a template structure composed of poly(lactic acid) swells and entraps a soluble polymer precursor. Once entrapped, the precursor is cured, resulting in a thin, conformal membrane. The thickness of each coating depends on the coating solution composition, residence time, and template size. Thicknesses ranged from 400 nm to 4 ?m within the experimental conditions we explored. The coating method was compatible with a range of polymers. Complicated 3D structures and microstructures of 10 ?m thickness and separation were coated using this technique. The templates can also be selectively removed, leaving behind a hollow membrane structure in the shape of the original printed, extruded, or microporous template structures. This technique may be useful in applications that benefit from three-dimensional membrane topologies, including catalysis, separations, and potentially tissue engineering.
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Unique patterns and proper management of postgastrectomy bleeding in patients with gastric cancer.
Surgery
PUBLISHED: 01-31-2014
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Bleeding after gastrectomy is a less frequent, but fatal complication. Because the pathophysiology and clinical manifestations differ considerably across cases, the exact outcome of postoperative bleeding is unclear. This study aimed to improve management practices for postgastrectomy bleeding by the analysis of risk factors and clinical patterns.
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Factors related to clinically relevant fatigue in disease-free stomach cancer survivors and expectation-outcome consistency.
Support Care Cancer
PUBLISHED: 01-09-2014
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Although early detection and improved treatment have increased the number of long-term survivors, little is known about the prevalence and associations of clinically relevant fatigue (CRF) in disease-free stomach cancer survivors. Because no effective CRF management strategy yet exists, understanding CRF risk factors is important for developing treatment approaches.
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MicroRNA 135a suppresses lymph node metastasis through down-regulation of ROCK1 in early gastric cancer.
PLoS ONE
PUBLISHED: 01-01-2014
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MicroRNAs (miRNAs) play a critical role in gastric cancer progression and metastasis. This study investigated the role of miRNA-135a in early gastric cancer (EGC) including lymph node (LN) metastasis. We examined the correlation between miRNA-135a expression and clinical outcomes in 59 patients who underwent surgery for EGC. Using gastric cancer cell lines, we performed functional and target gene analyses. miRNA-135a expression was down-regulated in 33.9% of patients. These patients showed a significantly more advanced stage (TNM stage ? IB, 35.0% vs. 12.8%, p?=?0.045) and higher rate of LN metastasis (30.0% vs. 5.1%, p?=?0.014) than those with up-regulation of miRNA-135a expression. In a multivariate analysis, down-regulation of miRNA-135a was an independent risk factor for LN metastasis (adjusted odds ratio, 8.04; 95% confidence interval, 1.08-59.81; p?=?0.042). Functional analyses using gastric cancer cell lines showed that miRNA-135a suppressed cell viability, epithelial-mesenchymal transition, cell invasion, and migration. ROCK1 was a target of miRNA-135a and its expression was inversely correlated to that of miRNA-135a. ROCK1 expression was significantly increased in EGC patients with LN metastasis than in those without LN metastasis. Our results confirm the tumor-suppressive role of miRNA-135a, and demonstrate its role in LN metastasis in EGC. miRNA-135a and its target gene ROCK1 may be novel therapeutic and prognostic targets for EGC.
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Clinical implication of sagittal translation in lengthening over nail of tibia.
Eur J Orthop Surg Traumatol
PUBLISHED: 08-22-2013
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During the surgery of lengthening over nail (LON), we sometimes observe sagittal translation of proximal tibia (STPT) when the nail is inserted into the canal. We investigated the factors that lead to STPT and its clinical significance.
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Laparoscopy Assisted versus Open Distal Gastrectomy with D2 Lymph Node Dissection for Advanced Gastric Cancer: Design and Rationale of a Phase II Randomized Controlled Multicenter Trial (COACT 1001).
J Gastric Cancer
PUBLISHED: 08-13-2013
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Laparoscopy-assisted distal gastrectomy for early gastric cancer has gained acceptance and popularity worldwide. However, laparoscopy-assisted distal gastrectomy for advanced gastric cancer is still controversial. Therefore, we propose this prospective randomized controlled multi-center trial in order to evaluate the safety and feasibility of laparoscopy assisted D2-gastrectomy for advanced stage gastric cancer.
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Proposal of the Surgical Options for Primary Tumor Control During Sentinel Node Navigation Surgery Based on the Discrepancy Between Preoperative and Postoperative Early Gastric Cancer Diagnoses.
Ann. Surg. Oncol.
PUBLISHED: 08-02-2013
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There is no consensus on the optimal method of primary tumor control, determined by preoperative clinical factors, during sentinel node (SN) navigation surgery for early gastric cancer (EGC). In this study, we investigated the accuracy of clinical diagnosis based on preoperative examination in patients with EGC and proposed surgical options for primary tumor control during SN navigation surgery.
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Association of smoking history with cancer recurrence and survival in stage III-IV male gastric cancer patients.
Cancer Epidemiol. Biomarkers Prev.
PUBLISHED: 07-31-2013
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Smoking and drinking alcohol are major risk factors for cancer development, and we investigated their effects on gastric cancer prognosis following initial resection.
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A Body Shape Index Has a Good Correlation with Postoperative Complications in Gastric Cancer Surgery.
Ann. Surg. Oncol.
PUBLISHED: 06-26-2013
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The relationship between obesity and surgical complications has been controversial. A Body Shape Index (ABSI) is a newly developed anthropometric index based on waist circumference adjusted for height and weight. The aim of this study was to investigate the relationship between ABSI and surgical complications.
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Decreased DBC1 Expression Is Associated With Poor Prognosis in Patients With Non-Muscle-Invasive Bladder Cancer.
Korean J Urol
PUBLISHED: 06-26-2013
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The deleted in bladder cancer 1 (DBC1) gene is located within chromosome 9 (9q32-33), a chromosomal region that frequently shows loss of heterozygosity in bladder cancer (BC). It is suspected that it acts as a tumor suppressor gene, but its prognostic value remains unclear. The aim of the present study was to investigate the value of DBC1 as a prognostic marker in BC.
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Painful patellar clunk or crepitation of contemporary knee prostheses.
Clin. Orthop. Relat. Res.
PUBLISHED: 05-23-2013
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Painful patellar clunk or crepitation (PCC) is a resurgent complication of contemporary posterior-stabilized TKA. The incidence, time to presentation, causes, and treatment of PCC still remain controversial.
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Repairing the annular ligament is not necessary in the operation of Mason type 2, 3 isolated radial head fractures if the lateral collateral ligament is intact: Minimum 5 years follow-up.
Injury
PUBLISHED: 05-16-2013
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The repair of annular ligament after open reduction and internal fixation of radial head fracture could produce the irritation or crepitation during range of motion exercise. The purpose of this study is to evaluate the significance of unrepaired annular ligament during fixation of isolated radial head fractures.
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Suicide ideation in stomach cancer survivors and possible risk factors.
Support Care Cancer
PUBLISHED: 05-05-2013
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Although the suicide rate of cancer survivors is higher than that of the general population, few studies have examined the potential risk factors. We assessed suicide ideation (SI) and investigated its correlates among survivors of stomach cancer, which is one of the most prevalent cancers in Korea.
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Outcome of endoscopic therapy for cancer bleeding in patients with unresectable gastric cancer.
J. Gastroenterol. Hepatol.
PUBLISHED: 04-23-2013
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Gastric cancer bleeding is not rare complication in patients with advanced gastric cancer (AGC). The aim of this study was to evaluate the efficacy and clinical outcomes of endoscopic therapy (ET) for upper gastrointestinal bleeding (UGIB) from unresectable AGC.
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Is there any role of additional retropancreatic lymph node dissection on D2 gastrectomy for advanced gastric cancer?
Ann. Surg. Oncol.
PUBLISHED: 03-30-2013
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Extended lymph node dissection beyond D2 in resectable gastric cancer has not shown any survival benefits. However, whether the retropancreatic (No. 13) lymph node should be dissected still remains controversial. The purpose of this study was to evaluate the effects of additional No. 13 lymph node dissection on D2 gastrectomy for gastric cancer in terms of overall survival.
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Clinical significance of intraperitoneal air on computed tomography scan after endoscopic submucosal dissection in patients with gastric neoplasms.
Surg Endosc
PUBLISHED: 03-29-2013
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Perforations are major complications of endoscopic gastric resection, including endoscopic submucosal dissection (ESD), and are generally detected on chest radiography following ESD. We hypothesized that a small amount of free air, defined as "intraperitoneal air," would not be noted on chest radiography. In this study we aimed to determine how often intraperitoneal air is seen on a computed tomography (CT) scan after ESD and to evaluate the association between clinical factors and intraperitoneal air.
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Improved survival after adding dissection of the superior mesenteric vein lymph node (14v) to standard D2 gastrectomy for advanced distal gastric cancer.
Surgery
PUBLISHED: 03-25-2013
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Extended lymph node dissection in gastric cancer (D3) was proven to have no survival benefit compared with a D2 dissection, but whether adding the superior mesenteric nodes (No. 14v) to the dissection provides survival benefit for gastric cancer patients remains controversial.
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Factors associated with depression in disease-free stomach cancer survivors.
J Pain Symptom Manage
PUBLISHED: 03-13-2013
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Depression in cancer survivors affects the rest of their lives in many ways.
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The risk factors for lymph node metastasis in early gastric cancer patients who underwent endoscopic resection: is the minimal lymph node dissection applicable? A retrospective study.
Surg Endosc
PUBLISHED: 02-15-2013
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The purpose of this study was to identify risk factors associated with lymph node (LN) metastasis in early gastric cancer patients who underwent endoscopic resection (ER) and to evaluate the feasibility of minimal LN dissection in these patients.
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Long-term outcomes of laparoscopy-assisted distal gastrectomy for early gastric cancer: result of a randomized controlled trial (COACT 0301).
Surg Endosc
PUBLISHED: 02-08-2013
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The purpose of this study was to evaluate laparoscopy-assisted distal gastrectomy (LADG) compared to open distal gastrectomy (ODG) in the treatment of early gastric cancer with respect to survival, surgical outcomes, complications, and quality of life (QOL).
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HOXA9, ISL1 and ALDH1A3 methylation patterns as prognostic markers for nonmuscle invasive bladder cancer: array-based DNA methylation and expression profiling.
Int. J. Cancer
PUBLISHED: 02-08-2013
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DNA methylation patterns are associated with the development and prognosis of cancer. The aim of this study was to identify novel methylation markers for the prediction of patient outcomes using microarray analysis of DNA methylation and RNA expression patterns in samples from long-term follow-up patients with nonmuscle invasive bladder cancer (NMIBC). A total of 187 human bladder specimens were used for microarray array or pyrosequencing (PSQ) analyses: 6 normal controls (NC) and 181 NMIBC. Tumor-specific hypermethylated genes were selected from a data set comprising 24 matched microarray-based DNA methylation and gene expression profiles (6 controls and 18 NMIBC), and their clinical relevance was verified by quantitative PSQ analysis. The methylation status of Homeobox A9 (HOXA9), ISL LIM homeobox 1 (ISL1) and Aldehyde dehydrogenase 1 family, member A3 (ALDH1A3) was significantly associated with decreased gene expression levels and aggressive clinicopathological characteristics. Multivariate regression analyses showed that hypermethylation of these genes was an independent predictor of disease recurrence (HOXA9, ISL1 and ALDH1A3, either alone or in combination) and progression (ISL1 and ALDH1A3, either alone or in combination) (each p?
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Recapitulation of previous genome-wide association studies with two distinct pathophysiological entities of gastric cancer in the Korean population.
J. Hum. Genet.
PUBLISHED: 02-07-2013
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Gastric cancer (GC) is the most common malignancy. The incidence rates remain remarkably high in East Asians. Although genome-wide association studies in the Han Chinese and Japanese populations have so far yielded susceptibility loci for GC, these findings need to be validated in an independent ethnic group. To identify the potential heterogeneity by histological classified subtypes (intestinal and diffuse), we examined the previously reported associations in the Korean population. PRKAA1 at 5p13.1 was found to be more strongly associated with intestinal type (odds ratio, OR=1.39, 95% CI (confidence interval) =1.22-1.58, P=3.77 × 10(-7)) than diffuse type. In addition, PSCA at 8q23.3 was significantly replicated in diffuse type (OR=1.49, 95% CI=1.32-1.67, P=2.43 × 10(-11)) but far less significant in intestinal type. In conclusion, these findings could bring additional insights into the etiologic heterogeneity in gastric carcinogenesis mechanisms.
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Discovering Medical Knowledge using Association Rule Mining in Young Adults with Acute Myocardial Infarction.
J Med Syst
PUBLISHED: 01-15-2013
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The knowledge discovery has been widely applied to mine significant knowledge from medical data. Nevertheless, previous studies have produced large numbers of imprecise patterns. To reduce the number of imprecise patterns, we need an approach that can discover interesting patterns that connote causality between antecedent and consequence in a pattern. In this paper, we propose association rule mining method that can discover interesting patterns that include medical knowledge in Korean acute myocardial infarction registry that consists of 1,247 young adults collected by 51 participating hospitals since 2005. Proposed method can remove imprecise patterns and discover target patterns that include associations between blood factors and disease history. The association that blood factors affect to disease history is defined as target pattern. In our experiments, the interestingness of a target pattern is evaluated in terms of statistical measures such as lift, leverage, and conviction. We discover medical knowledge that glucose, smoking, triglyceride total cholesterol, and creatinine are associated with diabetes and hypertension in Korean young adults with acute myocardial infarction.
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Synthesis of a new class of ?-iodo N-alkenyl 2-pyridones.
Org. Lett.
PUBLISHED: 11-09-2011
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A new method for the synthesis of ?-iodo N-alkenyl 2-pyridones from substituted 2-propargyloxypyridines has been discovered . These compounds present a unique complement of orthogonal functionality and structural characteristics that are unavailable via other routes. The ready access to these compounds renders them an important entry point for the preparation of more complex N-alkyl pyridone-containing targets.
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Design of sensor data processing steps in an air pollution monitoring system.
Sensors (Basel)
PUBLISHED: 09-27-2011
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Environmental monitoring is required to understand the effects of various kinds of phenomena such as a flood, a typhoon, or a forest fire. To detect the environmental conditions in remote places, monitoring applications employ the sensor networks to detect conditions, context models to understand phenomena, and computing technology to process the large volumes of data. In this paper, we present an air pollution monitoring system to provide alarm messages about potentially dangerous areas with sensor data analysis. We design the data analysis steps to understand the detected air pollution regions and levels. The analyzed data is used to track the pollution and to give an alarm. This implemented monitoring system is used to mitigate the damages caused by air pollution.
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Gastrogastric intussusception secondary to a gastric carcinoma: Report of a case.
Surg. Today
PUBLISHED: 09-16-2011
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Intussusception is uncommon in the proximal gastrointestinal tract, although a few case reports have been published on gastroduodenal intussusception associated with an anatomical abnormality or intussusceptions at a site of gastrojejunostomy after gastric surgery. We herein present a case of gastrogastric intussusception secondary to a gastric cancer, which presented as vomiting and poor oral intake. A 73-year-old woman was diagnosed with gastric cancer during a cancer screening by esophagogastroduodenoscopy and biopsy. Twenty days later, she presented with acute vomiting, a high fever, an increased heart rate, and severe anemia. Esophagogastroduodenoscopy demonstrated that the mass had been intussuscepted into the gastric lumen and distorted the distal antrum of the stomach. Standard distal subtotal gastrectomy was performed without reduction. Her postoperative course was uneventful.
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Reversible pulmonary hypertension in adolescent with left atrial myxoma.
J Cardiovasc Ultrasound
PUBLISHED: 08-21-2011
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We report a patient of left atrial huge myxoma presenting with severe pulmonary hypertension in adolescents. A patient was a 14-year-old boy presented with sudden onset dyspnea. Transthoracic echocardiographic study revealed the presence of a nodular, 4.34 × 8.11 cm sized, mobile, hyperechoic mass in the left atrium and severe pulmonary hypertension with tricuspid insufficiency. After surgical therapy, tricuspid regurgitation and pulmonary hypertension was decreased and the patient was stabilized and had an uneventful clinical course.
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Robot-assisted total gastrectomy is comparable with laparoscopically assisted total gastrectomy for early gastric cancer.
Surg Endosc
PUBLISHED: 07-20-2011
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Laparoscopically assisted total gastrectomy (LATG) is technically difficult. Robot surgery has theoretical advantages such as increased degrees of freedom of instruments and a three-dimensional view. The current study aimed to determine whether a robot-assisted total gastrectomy (RATG) has a real benefit over LATG in terms of surgical and oncologic outcomes.
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Arthroscopic suture anchor repair versus pullout suture repair in posterior root tear of the medial meniscus: a prospective comparison study.
Arthroscopy
PUBLISHED: 06-24-2011
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To evaluate functional and radiographic results of arthroscopic suture anchor repair for posterior root tear of the medial meniscus (PRTMM) and compare with pullout suture repair.
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Pretreatment risk factors for multiple gastric cancer and missed lesions.
J Surg Oncol
PUBLISHED: 06-07-2011
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Multiple gastric cancers (MGCs) were found in 4-10% of gastric cancer patients. The aim of this study was to identify pretreatment risk factors associated with MGC and missed lesions.
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Is the sentinel node biopsy clinically applicable for limited lymphadenectomy and modified gastric resection in gastric cancer? A meta-analysis of feasibility studies.
J Surg Oncol
PUBLISHED: 05-23-2011
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Sentinel node biopsies (SNBs) have been clinically applied in melanoma and breast cancer for limited lymphadenectomy. However, the use of SNB remains controversial in gastric cancer due to unsatisfactory sensitivity and variability. This meta-analysis was performed to determine the sensitivity of SNB in gastric cancer and to identify factors that improve its sensitivity.
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QSE: A new 3-D solvent exposure measure for the analysis of protein structure.
Proteomics
PUBLISHED: 04-13-2011
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Solvent exposure of amino acids measures how deep residues are buried in tertiary structure of proteins, and hence it provides important information for analyzing and predicting protein structure and functions. Existing methods of calculating solvent exposure such as accessible surface area, relative accessible surface area, residue depth, contact number, and half-sphere exposure still have some limitations. In this article, we propose a novel solvent exposure measure named quadrant-sphere exposure (QSE) based on eight quadrants derived from spherical neighborhood. The proposed measure forms a microenvironment around C? atom as a sphere with a radius of 13?Å, and subdivides it into eight quadrants according to a rectangular coordinate system constructed based on geometric relationships of backbone atoms. The number of neighboring C? atoms whose labels are the same is given as the QSE value of the center C? atom at hand. As evidenced by histograms that show very different distributions for different structure configurations, the proposed measure captures local properties that are characteristic for a residues eight-directional neighborhood within a sphere. Compared with other measures, QSE provides a different view of solvent exposure, and provides information that is specific for different tertiary structure. As the experimental results show, QSE measure can potentially be used in protein structure analysis and predictions.
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The lymphangiogenic factor SOX 18: a key indicator to stage gastric tumor progression.
Int. J. Cancer
PUBLISHED: 04-06-2011
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SOX group F genes are important regulators of angiogenesis and lymphangiogenesis. The aim of the present study was to examine the relationships between Sox group F expression and clinicopathological factors in gastric cancer. Three hundred and fifteen gastric cancer tissues and the corresponding normal gastric tissue were obtained from the tumor bank at the National Cancer Center, Korea. SOX group F mRNA levels in these tissues were evaluated by reverse transcriptase polymerase chain reaction (RT-PCR). The serum levels of SOX 18 proteins in 219 gastric cancer patients and in 30 healthy volunteers were also measured by enzyme-linked immunosorbent assay. Furthermore, immunohistochemistry (IHC) was performed on 679 gastric cancer tissues and the clinicopathological characteristics, as well as the survival rates of SOX 18 positive and negative gastric cancers were compared. RT-PCR showed that SOX group F mRNA was increased in the gastric cancer tissues compared to the normal gastric tissues (p < 0.001, respectively). The serum levels of SOX 18 protein were also increased in gastric cancer patients compared to healthy volunteers. IHC showed that of the 679 gastric cancer cases, 177 (26.1%) were positive for SOX 18 expression in their tumor stroma, and the frequencies of both lymphovascular invasion and lymph node metastases were higher in the SOX 18 positive than in the negative group. Both the 5-year survival and the recurrence-free survival were shorter for SOX 18 positive tumors (p = 0.023 and 0.012, respectively). SOX 18 expression might be a prognostic tumor marker and a potential therapeutic target in gastric cancer.
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Is the new seventh AJCC/UICC staging system appropriate for patients with gastric cancer?
J. Am. Coll. Surg.
PUBLISHED: 04-05-2011
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The purpose of this study was to compare the clinical usefulness of the seventh Union Internationale Contre le Cancer/American Joint Committee on Cancer (AJCC/UICC) staging system vs the sixth AJCC/UICC staging system in patients with gastric cancer.
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General perioperative management of gastric cancer patients at high-volume centers.
Gastric Cancer
PUBLISHED: 03-05-2011
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Gastric cancer is very common in Korea and Japan, where many hospitals annually perform high numbers of gastrectomies for gastric cancer. The aim of this study was to compare the general management of gastric cancer in high-volume centers in Korea and Japan.
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Survival analysis of gastric cancer patients with tumor thrombus in the portal vein.
J Surg Oncol
PUBLISHED: 02-22-2011
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A tumor thrombus in the portal vein originating from gastric cancer is a rare condition. Little is known about the development of portal vein tumor thrombus (PVTT) and its effect on the survival of gastric cancer patients.
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Conformational preferences of X-Pro sequences: Ala-Pro and Aib-Pro motifs.
J Phys Chem B
PUBLISHED: 10-19-2010
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Conformational preferences and prolyl cis-trans isomerizations of the X-Pro motifs (Ac-X-Pro-NHMe, X = Ala and Aib) are explored using the meta-hybrid functional M06-2X and the double-hybrid functional B2PLYP-D with empirical dispersion corrections in the gas phase and in water, where solvation free energies were calculated using the implicit SMD model. Ac-Ala-Pro-NHMe favors the type VI ?-turns in the gas phase and the open conformations in water. The populations of type VI ?-turns decrease from 71% in the gas phase to 21% in water, which is reasonably consistent with IR and NMR experimental results on tBoc-Ala-Pro-NHMe. However, Ac-Aib-Pro-NHMe prefers the type I ?-turns with ?-helical structures for both residues in the gas phase and in water, whose populations are estimated to be 66% in both phases. These calculated results may rationalize why most of the peptaibiotics containing the Aib-Pro sequence have a regular ?-helical conformation at the N- or C-terminus but a kinked ?-helical structure in the middle of the helix. The cis-trans isomerizations of the Ala-Pro and Aib-Pro peptide bonds proceed via the clockwise rotation with the different backbone conformations. The rotational barriers to cis-to-trans isomerization are estimated to be 19.73 kcal/mol for the Ala-Pro tripeptide and 16.64 kcal/mol for the Aib-Pro tripeptide in water, which indicates that the rotational barrier becomes lower by ~3 kcal/mol for the Aib-Pro peptide bond. The calculated rotational barrier for Ac-Ala-Pro-NHMe is consistent with the observed value of 19.3 kcal/mol for Suc-Ala-Ala-Pro-Phe-pNA from NMR experiments in a buffered solution.
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Anti-inflammatory and analgesic activities of SKLJI, a highly purified and injectable herbal extract of Lonicera japonica.
Biosci. Biotechnol. Biochem.
PUBLISHED: 10-07-2010
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The parenteral route has many merits over the oral route, including greater predictability, reproducibility of absorption, and rapid drug action, but injectable phytomedicines are uncommon due to protein precipitating tannin and hemolytic saponin components. In this study, in an effort to develop a safe injectable analgesic phytomedicine, we prepared a tannin and saponin-free Lonicera japonica extract, SKLJI, through fractionation and column purification, and evaluated its anti-inflammatory and analgesic activities in in vivo experimental models of inflammation and pain. The removal of tannin and saponin resulted in loganin and sweroside-enriched SKLJI and it showed reduced hemolysis and protein precipitation. In efficacy tests, SKLJI inhibited croton oil- and arachidonic acid-induced ear edema, acetic acid-induced writhing, and carrageenan-induced rat hind paw hyperalgesia. Inhibition of cylcooxygenase-2 (COX-2), inducible nitric oxide synthase (iNOS), and 5-lipoxyfenase (5-LO) activities by SKLJI appeared to be the mechanism underlying anti-inflammatory and analgesic efficacy. Loganin and sweroside also showed anti-inflammatory and analgesic activities, suggesting that they might be active principles in the efficacy of SKLJI. These results suggest that SKLJI is a viable candidate for a new anti-inflammatory and analgesic phytomedicine that can be administered by the parenteral route.
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Online multi-divisive hierarchical clustering for on-body sensor data.
Adv. Exp. Med. Biol.
PUBLISHED: 09-25-2010
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Data mining applications over on-body sensor data have earned great attention in recent years. We propose a novel Online Multi-divisive Hierarchical Clustering Method on on-body sensor data. Our method evolves tree-like top down hierarchy cluster, which splits and agglomerates clusters as needed. Experimental results prove a competing quality for our method over existing ones.
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Laparoscopy-assisted distal gastrectomy combined with laparoscopic spleen-preserving distal pancreatectomy for the treatment of early gastric cancer with pancreatic cystic neoplasm.
J Laparoendosc Adv Surg Tech A
PUBLISHED: 08-07-2010
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The use of laparoscopy-assisted distal gastrectomy (LADG) for early gastric cancer (EGC) and laparoscopic distal pancreatectomy (LDP) for lesions of benign or borderline malignancy have gained worldwide acceptance because they are viewed as safe and feasible. A 59-year-old man was diagnosed with EGC and intraductal papillary mucinous neoplasm (IPMN) simultaneously during cancer screening. LDP was performed prior to LADG due to the possibility of splenectomy. After completing LDP, LADG was performed in the usual manner. LADG combined with spleen-preserving LDP was performed safely. The operating time was 561 minutes, and there was no intraoperative complication. The patient was discharged on postoperative 10 without any complications. No recurrence or distant metastasis occurred during the subsequent 40 months. LADG combined with spleen-preserving LDP for EGC and IPMN was found to be feasible and less invasive than open surgery.
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Clinical outcomes and prognostic factors of metastatic gastric carcinoma patients who experience gastrointestinal perforation during palliative chemotherapy.
Ann. Surg. Oncol.
PUBLISHED: 06-29-2010
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We conducted the current study to investigate the clinical outcomes of metastatic gastric carcinoma (MGC) patients who experienced gastrointestinal (GI) perforation during palliative chemotherapy and to examine the prognostic factors associated with survival after perforation.
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Oncologic effectiveness of regular follow-up to detect recurrence after curative resection of gastric cancer.
Ann. Surg. Oncol.
PUBLISHED: 06-18-2010
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While clinicians routinely follow up gastric cancer patients after curative resection to detect recurrence, the effectiveness of regular follow-up has not been proven, and no consensus has been reached regarding follow-up programs.
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Helicobacter pylori Seropositivity Is Associated with Gastric Cancer Regardless of Tumor Subtype in Korea.
Gut Liver
PUBLISHED: 04-20-2010
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To evaluate the association between Helicobacter pylori (H. pylori) infection and gastric cancer (GC) according to tumor subtype in Korea.
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Hypoxic ischemia and proteasome dysfunction alter tau isoform ratio by inhibiting exon 10 splicing.
J. Neurochem.
PUBLISHED: 04-03-2010
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Alternative splicing of tau exon 10 influences microtubule assembly and stability during development and in pathological processes of the central nervous system. However, the cellular events that underlie this pre-mRNA splicing remain to be delineated. In this study, we examined the possibility that ischemic injury, known to change the cellular distribution and expression of several RNA splicing factors, alters the splicing of tau exon 10. Transient occlusion of the middle cerebral artery reduced tau exon 10 inclusion in the ischemic cortical area within 12 h, resulting in the induction of three-repeat (3R) tau in cortical neurons. Ubiquitinated protein aggregates and reduced proteasome activity were also observed. Administration of proteasome inhibitors such as MG132, proteasome inhibitor I and lactacystin reduced tau exon 10 splicing in cortical cell cultures. Decreased levels of Tra2beta, an RNA splicing factor responsible for tau exon 10 inclusion, were detected both in cortical cell cultures exposed to MG132 and in cerebral cortex after ischemic injury. Taken together, these findings suggest that transient focal cerebral ischemia reduces tau exon 10 splicing through a mechanism involving proteasome-ubiquitin dysfunction and down-regulation of Tra2beta.
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Predictors of timing and patterns of recurrence after curative resection for gastric cancer.
Dig Surg
PUBLISHED: 04-01-2010
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The aim of the study was to characterize recurrence patterns and identify predictors of recurrence after curative resection.
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A histidine substitution confers metal binding affinity to a Schistosoma japonicum Glutathione S-transferase.
Protein Expr. Purif.
PUBLISHED: 03-06-2010
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Glutathione S-transferases (GSTs) are multifunctional enzymes that are used as fusion tags on recombinant proteins in mammalian and Escherichia coli expression systems. We recently found that the Schistosoma japonicum GST (SjGST) displays weak Ni(2+) ion binding affinity. Glu26 and His79 were assumed to be its Ni(2+) binding sites based on the structure of the 26-kDa Clonorchis sinensis GST. To enhance SjGST Ni(2+) binding affinity, Glu26 was mutated to His. SjGST-E26H was expressed and purified at a high concentration of imidazole to a higher purity than wild type SjGST. In addition, human biotin protein ligase fused to SjGST-E26H was purified with a immobilized Ni affinity column.
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Prognostic value of preoperative clinical staging assessed by computed tomography in resectable gastric cancer patients: a viewpoint in the era of preoperative treatment.
Ann. Surg.
PUBLISHED: 02-25-2010
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In the era of pre- or perioperative therapy for gastric cancer, clinical staging before treatment appears to be increasingly important for prognosis, yet there are no data on the subject for resectable gastric cancer patients.
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Effective feature selection framework for cluster analysis of microarray data.
Bioinformation
PUBLISHED: 02-18-2010
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The microarray technique has become a standard means in simultaneously examining expression of all genes measured in different circumstances. As microarray data are typically characterized by high dimensional features with a small number of samples, feature selection needs to be incorporated to identify a subset of genes that are meaningful for biological interpretation and accountable for the sample variation. In this article, we present a simple, yet effective feature selection framework suitable for two-dimensional microarray data. Our correlation-based, nonparametric approach allows compact representation of class-specific properties with a small number of genes. We evaluated our method using publicly available experimental data and obtained favorable results.
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Pharmacokinetics of mirodenafil and its two metabolites, SK3541 and SK3544, in spontaneously or DOCA-salt-induced hypertensive rats.
Biopharm Drug Dispos
PUBLISHED: 02-01-2010
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Hypertension is the most common comorbidity and major risk factor in patients with erectile dysfunction. The pharmacokinetics of mirodenafil, used for the treatment of erectile dysfunction, after the intravenous and oral administration (20?mg/kg) to 6-week-old rats (with blood pressure within the normotensive range) and 16-week-old spontaneously hypertensive rats (SHRs) and their age-matched control normotensive Kyoto-Wistar (KW) rats, and 16-week-old deoxycorticosterone acetate-salt-induced hypertensive rats (DOCA-salt rats) and their age-matched control Sprague-Dawley (SD) rats were compared. It was found that time-averaged renal clearance (Cl(r)) was of minor importance and that time-averaged non-renal clearance (Cl(nr)) was dominant. In both 6- and 16-week-old SHRs, the Cl(nr)s and areas under the curve (AUCs) of intravenous mirodenafil were significantly smaller and greater than those of the controls, but in 16-week-old DOCA-salt rats, they were comparable to the controls. Although the AUC of oral mirodenafil in 16-week-old SHRs was comparable to the controls, the Cl(nr)s (or total body clearances, Cls) of intravenous mirodenafil and intestinal intrinsic clearances were significantly smaller than the controls and comparable to the controls for both 6- and 16-week-old SHRs, unlike in the 16-week-old DOCA-salt rats. The above data suggest that the significantly smaller Cl(nr) and greater AUC of intravenous mirodenafil and comparable AUC of oral mirodenafil in 16-week-old SHR could be due to the hereditary characteristics of SHRs, and not due to the hypertensive state itself.
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Self-expandable metallic stent placement for malignant obstruction in patients with locally recurrent gastric cancer.
Surg Endosc
PUBLISHED: 01-27-2010
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Self-expandable metallic stents (SEMSs) provide effective palliation for inoperable malignant gastric outlet obstruction (GOO). The objective of this study was to evaluate the effectiveness of SEMSs in patients with recurrent gastric cancer after radical gastrectomy.
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Covered versus uncovered self-expandable metallic stents for palliation of malignant pyloric obstruction in gastric cancer patients: a randomized, prospective study.
Gastrointest. Endosc.
PUBLISHED: 01-11-2010
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Self-expandable metallic stents (SEMSs) provide effective palliation of malignant pyloric obstruction in patients with inoperable gastric cancer.
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What is Visualize?

JoVE Visualize is a tool created to match the last 5 years of PubMed publications to methods in JoVE's video library.

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We use abstracts found on PubMed and match them to JoVE videos to create a list of 10 to 30 related methods videos.

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In developing our video relationships, we compare around 5 million PubMed articles to our library of over 4,500 methods videos. In some cases the language used in the PubMed abstracts makes matching that content to a JoVE video difficult. In other cases, there happens not to be any content in our video library that is relevant to the topic of a given abstract. In these cases, our algorithms are trying their best to display videos with relevant content, which can sometimes result in matched videos with only a slight relation.