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Find video protocols related to scientific articles indexed in Pubmed.
Influence of Pain Sensitivity on Surgical Outcomes after Lumbar Spine Surgery in Patients with Lumbar Spinal Stenosis.
Spine
PUBLISHED: 11-11-2014
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Study Design. Prospective observational studyObjectives. We aimed to assess the influence of pain sensitivity on surgical outcomes after lumbar spine surgery in patients with LSS.Summary of Background Data. No previous study has investigated the relationship between the surgical outcomes for lumbar spinal stenosis (LSS) and pain sensitivity questionnaire (PSQ) scores.Methods. The study included 171 patients who were scheduled to undergo spine surgery for LSS. Based on their PSQ scores, patients were assigned to either a low (PSQ score < 6.5, n = 87) or high PSQ group (PSQ score ? 6.5, n = 84). The primary outcome was the Oswestry Disability Index (ODI) at 12 months after surgery.Results. The ODI at 12 months after surgery was significantly lower in the low PSQ group than in the high PSQ group. Twelve months after surgery, the mean ODI scores (95% confidence interval [CI]) in the low and high PSQ groups were 21.1 (16.8-25.5) and 29.6 (25.0-34.1), respectively. The difference (95% CI) in the ODI between the 2 groups was 3.2 (-14.7 to -2.2) (P = 0.009). There were significant differences in the secondary endpoints, including the ODI and visual analogue scale (VAS) scores for back and leg pain, in the follow-up assessments over a 12-month period after surgery, between the 2 groups (PSQ group; P < 0.001 for the ODI, VAS score for back pain, and VAS score for leg pain). However, the patterns of changes of the ODI and VAS scores for back pain and leg pain in the follow-up assessments over a 12-month period were not significantly different (interaction between the PSQ group and follow-up assessment time; P = 0.757, 0.126, and 0.950, respectively).Conclusions. Patients with high pain sensitivity may display less improvement in back pain, leg pain, and disability after surgery for LSS as compared to patients with low pain sensitivity. Furthermore, the PSQ can be used to predict surgical outcomes after spine surgery for LSS.
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Nonmotor Symptoms and Cognitive Decline in de novo Parkinson's Disease.
Can J Neurol Sci
PUBLISHED: 11-07-2014
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Cognitive impairments are common in Parkinson's disease (PD). Despite its clinical importance, the development of dementia is still difficult to predict. In this study, we investigated the possible associations between non-motor symptoms and the risk of developing dementia within a 2-year observation period in PD.
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Triblock cylinders at fluid-fluid interfaces.
Langmuir
PUBLISHED: 11-04-2014
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We present the interactions and assembly of triblock cylinders at oil-water and air-water interfaces. ABA-type triblock cylinders with different block ratios and surface wettabilities are prepared using a micromolding method. These triblock cylinders at fluid-fluid interfaces induce complex interface deformation depending upon their relative block ratio and the surface wettability. It is observed that triblock cylinders generate octapolar interface deformation at the air-water interface, whereas the same cylinders cause quadrupolar deformation at the oil-water interface. Consequently, the interactions and assembly behavior of these triblock cylinders at each fluid interface strongly depend upon the nature of the interface deformation.
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Nonthermal Plasma Induces Apoptosis in ATC Cells: Involvement of JNK and p38 MAPK-Dependent ROS.
Yonsei Med. J.
PUBLISHED: 10-18-2014
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To determine the effects of nonthermal plasma (NTP) induced by helium (He) alone or He plus oxygen (O?) on the generation of reactive oxygen species (ROS) and cell death in anaplastic thyroid cancer cells.
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Effects of in vitro-growth culture duration on fertilizability of bovine growing oocytes and proliferation of cells surrounding oocytes.
Jpn. J. Vet. Res.
PUBLISHED: 10-07-2014
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The effects of in vitro-growth (IVG) culture duration (12 and 14 days) on the fertilizability and the developmental ability of IVG oocytes matured with pre-maturational culture, and on the proliferation of cells surrounding oocytes during IVG culture were examined. The fertilization and cleavage rates of 12- and 14-day IVG oocytes were similar; however, 14-day IVG oocytes showed lower blastocyst development rate than 12-day IVG oocytes. In addition, the number of cells surrounding oocytes increased until 12 days of IVG, but decreased at 14 days. The results indicated that the extension of IVG period beyond 12 days impaired the proliferation of cells surrounding oocytes, leading to the decrease of developmental ability but not fertilizability of 14-day IVG oocytes.
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Whitening Effect of Octaphlorethol A Isolated from Ishige foliacea in an in vivo Zebrafish Model.
J. Microbiol. Biotechnol.
PUBLISHED: 09-12-2014
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In a previous study, we isolated octaphlorethol A (OPA) from Ishige foliacea and evaluated its anti-melanogenesis activity in a murine melanoma cell line. However, the whitening effect and toxicity of OPA have not yet been examined in vivo. Therefore, in this study, we investigated the inhibitory effect of OPA on melanin synthesis and tyrosinase activity in an in vivo zebrafish model. More than 90% of subject embryos survived upon exposure to OPA concentrations below 25 µM, which was not significantly different from the finding in the control group. OPA markedly inhibited melanin synthesis and tyrosinase activity in a concentration-dependent manner.
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Crystal structure of 2,6-bis-[(1H-pyrazol-1-yl)meth-yl]pyridine.
Acta Crystallogr Sect E Struct Rep Online
PUBLISHED: 09-01-2014
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In the title compound, C13H13N5, the planes of the pyrazolyl groups are nearly perpendicular to that of the central pyridine ring, making dihedral angles of 87.77?(8) and 85.73?(7)°. In the crystal, weak C-H?N hydrogen bonds link the mol-ecules into layers extending parallel to (10-1).
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Crystal structure of phen-yl(pyridin-2-yl)methanol.
Acta Crystallogr Sect E Struct Rep Online
PUBLISHED: 09-01-2014
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In the title compound, C12H11NO, the pyridine and phenyl rings are inclined to each other by 71.42?(10)°. In the crystal, O-H?N hydrogen bonds link the mol-ecules into helical chains extending along the c-axis direction.
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Crystal structure of a monoclinic polymorph of 5-amino-1,3,4-thia-diazol-2(3H)-one.
Acta Crystallogr Sect E Struct Rep Online
PUBLISHED: 09-01-2014
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The title compound, C2H3N3OS, is a monoclinic (P21/c) polymorph of the previously reported triclinic structure [Kang et al. (2012 ?). Acta Cryst. E68, o1198]. The asymmetric unit contains two independent mol-ecules which are essentially planar, with r.m.s. deviations of 0.001 and 0.032?Å from the mean plane defined by the seven non-H atoms. In the crystal, N-H?N and N-H?O hydrogen bonds link the mol-ecules into a sheet parallel to (111).
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Crystal structure of chlorido-penta-kis(dimethyl sulfoxide-?O)chromium(III) dichloride.
Acta Crystallogr Sect E Struct Rep Online
PUBLISHED: 09-01-2014
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In the complex cation of the title salt, [CrCl(C2H6OS)5]Cl2, the Cr(III) ion is coordinated by one chloride ligand and five O atoms from dimethyl sulfoxide (DMSO) ligands, leading to a slightly distorted octa-hedral coordination environment [O-Cr-O angles range from 86.69?(16) to 92.87?(16)°]. In the crystal, complex cations are arranged in hexa-gonally packed rows parallel to [010], with the chloride counter-anions situated in between. The inter-actions between cations and anions are mainly ionic in nature.
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Octaphlorethol A: a potent ?-glucosidase inhibitor isolated from Ishige foliacea shows an anti-hyperglycemic effect in mice with streptozotocin-induced diabetes.
Food Funct
PUBLISHED: 08-22-2014
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?-Glucosidase inhibitors are important agents for decreasing postprandial hyperglycemia. The current study examined the inhibitory effects of octaphlorethol A (OPA) isolated from Ishige foliacea, a brown alga, on ?-glucosidase, and analyzed the inhibitor's binding modes using the crystal structure of ?-glucosidase. The effects of OPA on postprandial blood glucose levels after meals were also investigated. The IC50 value of OPA against ?-glucosidase was 0.11 mM, which is higher than that of the commercial inhibitor acarbose. For further insights, we predicted the 3D structure of ?-glucosidase and used a docking algorithm to simulate binding between ?-glucosidase and OPA. These molecular modeling studies were successful, and indicated that OPA interacts with Phe575, His600, Arg526, Met444, Asp542, Tyr605, Ser448, Asp203, Lys480, and Phe450. Furthermore, increases in postprandial blood glucose levels were significantly suppressed in the OPA-treated group compared with those in the streptozotocin-induced diabetic or normal mice. Additionally, the area under the curve was significantly reduced following OPA administration (907 versus 1034 mg h dL(-1)) in the diabetic mice, along with a delay in the absorption of dietary carbohydrates. Collectively, these results indicated that OPA is a potent inhibitor of ?-glucosidase, and shows potential to be used as an anti-diabetic agent.
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Analysis of cell growth and gene expression of porcine adipose tissue-derived mesenchymal stem cells as nuclear donor cell.
Dev. Growth Differ.
PUBLISHED: 08-16-2014
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In several laboratory animals and humans, adipose tissue-derived mesenchymal stem cells (ASC) are of considerable interest because they are easy to harvest and can generate a huge proliferation of cells from a small quantity of fat. In this study, we investigated: (i) the expression patterns of reprogramming-related genes in porcine ASC; and (ii) whether ASC can be a suitable donor cell type for generating cloned pigs. For these experiments, ASC, adult skin fibroblasts (AF) and fetal fibroblasts (FF) were derived from a 4-year-old female miniature pig. The ASC expressed cell-surface markers characteristic of stem cells, and underwent in vitro differentiation when exposed to specific differentiation-inducing conditions. Expression of DNA methyltransferase (DNMT)1 in ASC was similar to that in AF, but the highest expression of the DNMT3B gene was observed in ASC. The expression of OCT4 was significantly higher in FF and ASC than in AF (P < 0.05), and SOX2 showed significantly higher expression in ASC than in the other two cell types (P < 0.05). After somatic cell nuclear transfer (SCNT), the development rate of cloned embryos derived from ASC was comparable to the development of those derived using FF. Total cell numbers of blastocysts derived using ASC and FF were significantly higher than in embryos made with AF. The results demonstrated that ASC used for SCNT have a potential comparable to those of AF and FF in terms of embryo in vitro development and blastocyst formation.
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Relationship between cervical sagittal alignment and quality of life in ankylosing spondylitis.
Eur Spine J
PUBLISHED: 08-12-2014
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Little information is available on the relationship between cervical sagittal alignment and health-related quality of life (HRQOL) in ankylosing spondylitis (AS) patients. The aim of this study was to identify relationships between cervical sagittal alignment and HRQOL in AS.
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Origin of Leakage Paths Driven by Electric Fields in Al-Doped TiO2 Films.
Adv. Mater. Weinheim
PUBLISHED: 08-09-2014
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The growth of leakage current paths in Al-doped TiO2 (ATO) films is observed by in situ TEM under negative bias stress. Through systematic HAADF-STEM, STEM-EDS, and STEM-EELS studies, it is confirmed that the electric field-induced growth of the Ru-doped TiO2 phase is the main reason for the ATO film's negative leakage.
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Metachronous isolated breast metastasis from pulmonary adenocarcinoma with micropapillary component causing diagnostic challenges.
BMC Cancer
PUBLISHED: 07-15-2014
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Breast metastasis from extramammary malignancy is uncommon and often presents diagnostic challenges. Herein, we report a case of a patient with metachronous isolated breast metastasis from pulmonary adenocarcinoma with micropapillary component.
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Anti-Inflammatory Activity of Questinol Isolated from Marine-Derived Fungus Eurotium amstelodami in Lipopolysaccharide-Stimulated RAW 264.7 Macrophages.
J. Microbiol. Biotechnol.
PUBLISHED: 07-03-2014
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In the present study, an anthraquinone derivative, questinol was successfully isolated from the broth extract of the marine-derived fungus Eurotium amstelodami for the first time. The structure of questinol was determined based on the analysis of the MS and NMR spectral data as well as comparison of those data with the published data. Moreover, the anti-inflammatory effect of questinol in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells was investigated. The results showed that questinol did not exhibit cytotoxicity in LPS-stimulated RAW 264.7 cells up to 200 µM. Questinol could significantly inhibit NO and PGE2 production at indicated concentrations. Questinol was also found to inhibit the production of pro-inflammatory cytokines, including TNF-?, IL-1?, and IL-6. Furthermore, the western blot analysis showed that questinol suppressed the expression level of iNOS in a dose-dependent manner. However, questinol could slightly inhibit the expression of COX-2 at the concentration of 200 µM. Therefore, our study suggests that questinol might be selected as a promising agent for the prevention and therapy of inflammatory disease.
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Median nerve T2 assessment in the wrist joints: preliminary study in patients with carpal tunnel syndrome and healthy volunteers.
J Magn Reson Imaging
PUBLISHED: 06-14-2014
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To perform a prospective quantitative analysis of median nerve T2 values and cross-sectional area (CSA) in patients with carpal tunnel syndrome (CTS) as compared to asymptomatic volunteers.
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A Surgical Method for Determining Proper Screw Length in ACDF.
Korean J Spine
PUBLISHED: 06-10-2014
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We describe a surgical tool that uses the distractor pin as a reference for determining proper screw length in ACDF. It is critical that screw purchase depth be as deep as possible without violating or penetrating the posterior cortical wall, which ensures strong pull out strength.
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Effect of Reoperation on Long-Term Outcome of pT1b/T2 Gallbladder Carcinoma After Initial Laparoscopic Cholecystectomy.
J. Gastrointest. Surg.
PUBLISHED: 05-31-2014
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A small proportion of gallbladder carcinomas (GBC) are incidentally detected after laparoscopic cholecystectomy (LC). This study intended to analyze the effect of extended reoperation on the long-term outcome of patients with pT1b/T2 GBC who had initially undergone LC.
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MicroRNAs in human lung cancer.
Exp. Biol. Med. (Maywood)
PUBLISHED: 05-28-2014
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Lung cancer, which can be divided into two major clinical-pathological categories, small cell lung cancer and non-small cell lung cancer, is the leading cause of cancer-related death worldwide. MicroRNAs (miRNAs), small non-coding RNAs approximately 22 nucleotides in length, have been reported to be upregulated or downregulated in disease states and specific cell types. Recently, miRNAs have gained recognition as major regulators of human gene expression. MiRNAs can control highly complex signal transduction pathways and other biological pathways by targeting and controlling gene expression, accounting for their important role in lung cancer. Findings from recent studies on the roles of miRNAs in lung cancer are summarized in this review. Understanding miRNA functions in lung cancer will bring molecular-level insight leading to better prognosis, diagnosis, and therapeutic approaches.
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Section 15. A desensitizing protocol without local graft infusion therapy and splenectomy is a safe and effective method in ABO-incompatible adult LDLT.
Transplantation
PUBLISHED: 05-23-2014
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The use of rituximab (Rit) to prevent antibody-mediated rejection (AMR) of ABO-incompatible (ABOi) adult living donor liver transplants (ALDLTs) has raised questions about the role of local graft infusion therapy (LGIT) and splenectomy (SPN); however, they are still regarded as essential components of the desensitization (DSZ) protocol.
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Association between serum adipocytokine levels and microangiopathies in patients with type 2 diabetes mellitus.
J Diabetes Investig
PUBLISHED: 05-21-2014
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It is thought that adipocytokines contribute to the increased risk of vascular complications in type 2 diabetes. However, there is still limited information on the relationship between microangiopathies and adipocytokines, such as adiponectin, leptin and tumor necrosis factor-? (TNF-?) in patients with type 2 diabetes.
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Prevalences of diabetic retinopathy and nephropathy are lower in Korean type 2 diabetic patients with non-alcoholic fatty liver disease.
J Diabetes Investig
PUBLISHED: 05-21-2014
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The associations between non-alcoholic fatty liver disease (NAFLD) and chronic vascular complications of type 2 diabetes remain uncertain. We assessed the relationships between NAFLD and chronic vascular complications in patients with type 2 diabetes.
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Beauty of lotus is more than skin deep: highly buoyant superhydrophobic films.
ACS Appl Mater Interfaces
PUBLISHED: 05-13-2014
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We develop highly buoyant superhydrophobic films that mimic the three-dimensional structure of lotus leaves. The high buoyancy of these structure stems from mechanically robust bubbles that significantly reduce the density of the superhydrophobic films. These highly buoyant superhydrophobic films stay afloat on water surface while carrying a load that is more than 200 times their own weight. In addition to imparting high buoyancy, the incorporation of robust hydrophilic bubbles enables the formation of free-standing structures that mimic the water-collection properties of Namib Desert beetle. We believe the incorporation of robust bubbles is a general method that opens up numerous possibilities in imparting high buoyancy to different structures that needs to stay afloat on water surfaces and can potentially be used for the fabrication of lightweight materials. (Image on the upper left reproduced with permission from Yong, J.; Yang, Q.; Chen, F.; Zhang, D.; Du, G.; Si, J.; Yun, F.; Hou, X. A Bioinspired Planar Superhydrophobic Microboat. J. Micromech. Microeng. 2014, 24, 035006. Copyright 2014 IOP Publishing.).
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Statins improve survival in patients with cardioembolic stroke.
Stroke
PUBLISHED: 05-06-2014
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The objective of this study was to investigate the potential benefits of statin therapy on mortality and stroke recurrence after cardioembolic stroke.
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Anterior lumbar interbody fusion for the treatment of postoperative spondylodiscitis.
J Korean Neurosurg Soc
PUBLISHED: 05-05-2014
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To analyze the clinical courses and outcomes after anterior lumbar interbody fusion (ALIF) for the treatment of postoperative spondylodiscitis.
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Prediabetes is not independently associated with microalbuminuria in Korean general population: The Korea National Health and Nutrition Examination Survey 2011-2012 (KNHANES V-2,3).
Diabetes Res. Clin. Pract.
PUBLISHED: 04-22-2014
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We investigated the association between microalbuminuria and prediabetes in Korean population using data from the KNHANES 2011-2012. Prevalence of microalbuminuria was significantly increased in prediabetes group. However, the odds ratio became insignificant after adjustment for blood pressure, and the prevalence of microalbuminuria was not increased in prediabetic subjects without hypertension.
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Short-term outcomes of immediate breast reconstruction using an implant or tissue expander after mastectomy in breast cancer patients.
Breast Cancer
PUBLISHED: 04-18-2014
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Mastectomy is an optional surgical management of breast cancer, but it can cause significant adverse reactions. Breast reconstruction is a concern in post-mastectomy recovery. We assessed the oncologic safety and patient satisfaction following immediate breast reconstruction using an implant or tissue expander.
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A clinical analysis of sudden sensorineural hearing loss cases.
Korean J Audiol
PUBLISHED: 04-17-2014
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High-dose systemic steroid therapy is the mainstay treatment for sudden sensorineural hearing loss (SSNHL). Recovery rates from SSNHL range are about 47-63% and are influenced by various prognostic factors. To evaluate the prognostic value of specific clinical parameters, we reviewed 289 cases by clinical and statistical analysis.
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Clinical practice guidelines for the medical and surgical management of primary intracerebral hemorrhage in Korea.
J Korean Neurosurg Soc
PUBLISHED: 04-16-2014
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The purpose of this clinical practice guideline (CPG) is to provide current and comprehensive recommendations for the medical and surgical management of primary intracerebral hemorrhage (ICH). Since the release of the first Korean CPGs for stroke, evidence has been accumulated in the management of ICH, such as intracranial pressure control and minimally invasive surgery, and it needs to be reflected in the updated version. The Quality Control Committee at the Korean Society of cerebrovascular Surgeons and the Writing Group at the Clinical Research Center for Stroke (CRCS) systematically reviewed relevant literature and major published guidelines between June 2007 and June 2013. Based on the published evidence, recommendations were synthesized, and the level of evidence and the grade of the recommendation were determined using the methods adapted from CRCS. A draft guideline was scrutinized by expert peer reviewers and also discussed at an expert consensus meeting until final agreement was achieved. CPGs based on scientific evidence are presented for the medical and surgical management of patients presenting with primary ICH. This CPG describes the current pertinent recommendations and suggests Korean recommendations for the medical and surgical management of a patient with primary ICH.
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Contribution of subcutaneous abdominal fat on ultrasonography to carotid atherosclerosis in patients with type 2 diabetes mellitus.
Cardiovasc Diabetol
PUBLISHED: 03-26-2014
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Whereas visceral abdominal adipose tissue (VAT) is associated with cardiometabolic risk, there is debate regarding the role of subcutaneous abdominal adipose tissue (SAT). The aim of this study was to investigate the relationships of subcutaneous and visceral abdominal fat with carotid atherosclerosis in patients with type 2 diabetes mellitus (T2DM).
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Is reduced arm and leg swing in Parkinson's disease associated with rigidity or bradykinesia?
J. Neurol. Sci.
PUBLISHED: 03-18-2014
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Arm and leg swings during gait are reduced and asymmetric in Parkinson's disease (PD). Although rigidity and bradykinesia are interconnected with each other, and related with gait hypokinesia including arm and leg swing alteration, it remains uncertain which factor is more responsible for the decrease of arm and leg swings. The study aimed to uncover which factor between rigidity and bradykinesia is more associated with the reduction of arm and leg swings during gait.
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The association between psychiatric factors and the development of chronic dysphagia after anterior cervical spine surgery.
Eur Spine J
PUBLISHED: 03-15-2014
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Little data are available regarding the influence of psychiatric factors on chronic dysphagia after anterior cervical spine surgery. The purpose of this study was to identify associations between psychiatric factors and the development of chronic dysphagia in patients after anterior cervical spine surgery.
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Pancreatic atrophy relative to external versus internal drainage of the pancreatic duct after pylorus-preserving pancreaticoduodenectomy.
J. Gastrointest. Surg.
PUBLISHED: 03-08-2014
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Atrophy of the pancreatic parenchyma, which occurs frequently after pylorus-preserving pancreaticoduodenectomy (PPPD), is often associated with pancreatic exocrine insufficiency. Many surgeons prefer to insert a drainage tube into the remnant pancreatic duct primarily to prevent pancreatic leakage at the pancreaticojejunostomy (PJ) after PPPD. Drainage methods vary widely but can be roughly classified as internal or external drainage. This study intended to evaluate their effects on pancreatic parenchymal atrophy following PPPD.
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The efficacy of the well of the well (WOW) culture system on development of bovine embryos in a small group and the effect of number of adjacent embryos on their development.
Zygote
PUBLISHED: 03-07-2014
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Summary The aim of the present study was to clarify the efficacy of the well of the well (WOW) culture system for a small number of embryos and the effect of number of adjacent embryos in a WOW dish on blastocyst development. In conventional droplet culture, embryos in the small-number group (5-6 embryos/droplet) showed low blastocyst development compared with a control group (25-26 embryos/droplet). However, small and large numbers of embryos (5-6 and 25 embryos, respectively) in a WOW dish showed no significant differences in cleavage, blastocyst rates, and mean cell number in blastocysts compared with the control group (25-30 embryos/droplet). In addition, the number of adjacent embryos in a WOW dish did not affect the development to blastocysts and cell number in blastocysts. In conclusion, a WOW dish can provide high and stable blastocyst development in small group culture wherever embryos are placed in microwells of the WOW dish.
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A rapid one-step fabrication of patternable superhydrophobic surfaces driven by Marangoni instability.
Langmuir
PUBLISHED: 03-06-2014
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We present a facile and inexpensive approach without any fluorinated chemistry to create superhydrophobic surface with exceptional liquid repellency, transportation of oil, selective capture of oil, optical bar code, and self-cleaning. Here we show experimentally that the control of evaporation is important and can be used to form superhydrophobic surface driven by Marangoni instability: the method involves in-situ photopolymerization in the presence of a volatile solvent and porous PDMS cover to afford superhydrophobic surfaces with the desired combination of micro- and nanoscale roughness. The porous PDMS cover significantly affects Marangoni convection of coating fluid, inducing composition gradients at the same time. In addition, the change of concentration of ethanol is able to produce versatile surfaces from hydrophilic to superhydrophobic and as a consequence to determine contact angles as well as roughness factors. In conclusion, the control of evaporation under the polymerization provides a convenient parameter to fabricate the superhydrophobic surface, without application of fluorinated chemistry and the elegant nanofabrication technique.
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Complex ordered patterns in mechanical instability induced geometrically frustrated triangular cellular structures.
Phys. Rev. Lett.
PUBLISHED: 03-05-2014
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Geometrical frustration arises when a local order cannot propagate throughout the space because of geometrical constraints. This phenomenon plays a major role in many systems leading to disordered ground-state configurations. Here, we report a theoretical and experimental study on the behavior of buckling-induced geometrically frustrated triangular cellular structures. To our surprise, we find that buckling induces complex ordered patterns which can be tuned by controlling the porosity of the structures. Our analysis reveals that the connected geometry of the cellular structure plays a crucial role in the generation of ordered states in this frustrated system.
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Treatment of progressive supranuclear palsy with autologous adipose tissue-derived mesenchymal stem cells: a case report.
J Med Case Rep
PUBLISHED: 03-04-2014
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Progressive supranuclear palsy is a relentlessly progressive neurodegenerative disorder and is clinically characterized by parkinsonism. Adipose tissue-derived mesenchymal stem cells have recently demonstrated the possibility of treating neurological disorders. Therefore, autologous adipose tissue-derived mesenchymal stem cells transplantation can be an alternative method for treating progressive supranuclear palsy.
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Clinical Characteristics and Metabolic Features of Patients with Adrenal Incidentalomas with or without Subclinical Cushing's Syndrome.
Endocrinol Metab (Seoul)
PUBLISHED: 02-28-2014
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The aim of this study was to examine the clinical characteristics of adrenal incidentalomas discovered by computed tomography (CT) and to investigate metabolic features of subclinical Cushing's syndrome (SCS) in patients with adrenal incidentalomas in a tertiary hospital in Korea.
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Metastasis of colon cancer to medullary thyroid carcinoma: a case report.
J. Korean Med. Sci.
PUBLISHED: 02-21-2014
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Metastasis to the primary thyroid carcinoma is extremely rare. We report here a case of colonic adenocarcinoma metastasis to medullary thyroid carcinoma in a 53-yr old man with a history of colon cancer. He showed a nodular lesion, suggesting malignancy in the thyroid gland, in a follow-up examination after colon cancer surgery. Fine needle aspiration biopsy (FNAB) of the thyroid gland showed tumor cell clusters, which was suspected to be medullary thyroid carcinoma (MTC). The patient underwent a total thyroidectomy. Using several specific immunohistochemical stains, the patient was diagnosed with colonic adenocarcinoma metastasis to MTC. To the best of our knowledge, the present patient is the first case of colonic adenocarcinoma metastasizing to MTC. Although tumor-tumor metastasis to primary thyroid carcinoma is very rare, we still should consider metastasis to the thyroid gland, when a patient with a history of other malignancy presents with a new thyroid finding.
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Risk of Malignancy in Thyroid Incidentalomas Identified by Fluorodeoxyglucose-Positron Emission Tomography.
Endocrinol Metab (Seoul)
PUBLISHED: 02-20-2014
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Thyroid incidentalomas detected by 2-deoxy-2-18F-fluoro-D-glucose positron emission tomography/computed tomography (18F-FDG PET/CT) have been reported in 1% to 4% of the population, with a risk of malignancy of 27.8% to 74%. We performed a retrospective review of FDG-avid thyroid incidentalomas in cancer screening subjects and patients with nonthyroid cancer. The risk of malignancy in thyroid incidentaloma and its association with the maximal standardized uptake value (SUVmax) in 18F-FDG PET/CT were evaluated to define the predictor variables in assessing risk of malignancy.
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Differential associations of central and brachial blood pressure with carotid atherosclerosis and microvascular complications in patients with type 2 diabetes.
BMC Cardiovasc Disord
PUBLISHED: 02-17-2014
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We examined the relationship between central blood pressure (BP), brachial BP with carotid atherosclerosis and microvascular complications in type 2 diabetes mellitus (T2DM).
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Protective effect of fucoidan against AAPH-induced oxidative stress in zebrafish model.
Carbohydr Polym
PUBLISHED: 02-11-2014
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Fucoidan, extracted from Ecklonia cava, has been extensively studied because of its wide biological activities. However, antioxidative activities have not been yet examined. Therefore we evaluated in vitro and in vivo studies on antioxidative activities of E. cava fucoidan (ECF). ECF exhibited more prominent effects in peroxyl radical scavenging activity, compared to the other scavenging activities. Thus, ECF was further evaluated for its protective ability against 2,2'-azobis dihydrochloride induced oxidative stress in Vero cells and ECF strongly reduced the AAPH-induced oxidative damage through scavenging intracellular reactive oxygen species. Furthermore, we evaluated protective effect of ECF against AAPH-induced oxidative stress in zebrafish model. ECF significantly reduced ROS generation, lipid peroxidation and cell death in zebrafish model. These findings indicate that ECF has antioxidant activities in vitro Vero cells and in vivo zebrafish model, even though ECF is not a polyphenol or flavonoid compound and does not contain benzene rings or conjugated structures.
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Biliary stricture is the only concern in ABO-incompatible adult living donor liver transplantation in the rituximab era.
J. Hepatol.
PUBLISHED: 02-06-2014
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With the introduction of rituximab prophylaxis, the survival of ABO-incompatible (ABOi) adult living donor liver transplant (ALDLT) has been strikingly improved due to the decreased incidence of antibody-mediated rejection. However, biliary stricture (BS) related to ABO incompatibility remains an unresolved concern.
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Chromene suppresses the activation of inflammatory mediators in lipopolysaccharide-stimulated RAW 264.7 cells.
Food Chem. Toxicol.
PUBLISHED: 02-06-2014
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Inflammation is complex process involving a variety of immune cells that defend the body from harmful stimuli. However, pro-inflammatory cytokines and inflammatory mediators can also exacerbate diseases such as cancer. The aim of this study was to identify a natural effective remedy for inflammation. We isolated a functional algal chromene compound from Sargassum siliquastrum, named sargachromanol D (SD). We evaluated the anti-inflammatory effect of SD on lipopolysaccharide (LPS)-exposed RAW 264.7 cells by measuring cell viability, cytotoxicity, and production of inflammatory mediators such as nitric oxide (NO), prostaglandin E2 (PGE2), inducible NO synthase (iNOS), cyclooxygenase-2 (COX-2), and the pro-inflammatory cytokines tumor necrosis factor-? (TNF-?), interleukin (IL)-1?, and IL-6. SD inhibited production of NO and PGE2 from LPS-induced cells by preventing the expression of inflammatory mediators such as iNOS and COX-2 in a dose-dependent manner. Concurrently, levels of the pro-inflammatory cytokines TNF-?, IL-1?, and IL-6 were reduced with increasing concentrations of SD. In addition, SD inhibited the activation of NF-?B and mitogen-activated protein kinases (MAPKs) pathways in a concentration-dependent manner. These results indicate that SD inhibits LPS-stimulated inflammation by inhibition of the NF-?B and MAPKs pathways in macrophages.
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Aging-related changes in in vitro-matured bovine oocytes: oxidative stress, mitochondrial activity and ATP content after nuclear maturation.
J. Reprod. Dev.
PUBLISHED: 02-04-2014
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The objective of this research was to clarify the aging-related changes in in vitro-matured bovine oocytes. Firstly, we examined the fertilization and embryonic development of bovine oocytes after 22 and 30-34 h of in vitro maturation (IVM). The oocytes after 30-34 h of IVM (penetrated by sperm at around 40 h after starting IVM) showed a lower developmental rate to blastocysts (P<0.01), although normal fertilization rates were similar regardless of IVM duration. In the next experiment, reactive oxygen species (ROS), mitochondrial activity and ATP content in oocytes after 20, 30 and 40 h of IVM were examined. The lowest level of ROS was found in the group subjected to 30 h of IVM. The mitochondrial activity and ATP content in the group subjected to 40 h of IVM were higher than in the group subjected to 20 h of IVM (P<0.01), and those in the group subjected to 30 h of IVM showed intermediate values. Thereafter, the mitochondrial activities at 3 days after in vitro fertilization in embryos derived from the oocytes subjected to 22 and 34 h of IVM were evaluated. In the group subjected to 34 h of IVM, high-polarized mitochondria were frequently observed at the periphery of blastomeres. The present results suggest that high mitochondrial activity observed in oocytes after prolonged IVM culture and localization of high-polarized mitochondria at the periphery of blastomeres during early embryonic development may be associated with the low developmental competence in aged bovine oocytes.
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(5-Methyl-pyrazine-2-carboxyl-ato-?(2) N (1),O)bis-[2-(4-methyl-pyridin-2-yl-?N)-3,5-bis-(tri-fluoro-meth-yl)phenyl-?C (1)]iridium(III) chloro-form hemisolvate.
Acta Crystallogr Sect E Struct Rep Online
PUBLISHED: 02-01-2014
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In the title complex, [Ir(C14H8F6N)2(C6H5N2O2)]·0.5CHCl3, the Ir(III) atom adopts a distorted octa-hedral geometry, being coordinated by three N atoms (arranged meridionally), two C atoms and one O atom of three bidentate ligands. The complex mol-ecules pack with no specific inter-molecular inter-actions between them. The SQUEEZE procedure in PLATON [Spek (2009 ?). Acta Cryst. D65, 148-155] was used to model a disordered chloro-form solvent mol-ecule; the calculated unit-cell data allow for the presence of half of this mol-ecule in the asymmetric unit.
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Estimation of the optimal timing of fertilization for embryo development of in vitro-matured bovine oocytes based on the times of nuclear maturation and sperm penetration.
J. Vet. Med. Sci.
PUBLISHED: 01-16-2014
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The objective of this research was to estimate the optimal timing for fertilization to achieve proper embryonic development of in vitro-matured bovine oocytes. First, cumulus-oocyte complexes were subjected to in vitro maturation (IVM) for 14-22 hr. The timing when 50% of oocytes reached metaphase II stage was estimated to be 17.5 hr after IVM start. Next, using oocytes subjected to IVM for 12-30 hr, sperm penetration was examined after 4-18 hr of in vitro fertilization (IVF). A significant negative correlation between IVM duration and the timing when 50% of oocytes were penetrated by sperm after IVF start was observed (P<0.01). Finally, oocytes subjected to 12-30 hr of IVM were inseminated and cultured for 6 days to examine embryonic development. In the group with 22 hr of IVM, the percentages of cleaved embryos and blastocysts were the highest values in all groups. According to the regression equation describing the time from nuclear maturation to sperm penetration (x) and the percentage of blastocysts (y) (y=7.23x - 0.297x(2), P<0.01), the blastocyst rate peaked when sperm penetration occurred at 12.2 hr after achieving nuclear maturation. In conclusion, under the present IVM/IVF conditions, it was estimated that oocytes acquired their highest developmental competence at about 30 hr after IVM start, and thus, the optimal IVM duration was calculated to be about 21 hr.
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Validation of the Quality-of-Life Questionnaire of the European Foundation for Osteoporosis (QUALEFFO-26) in Korean population.
Rheumatol. Int.
PUBLISHED: 01-11-2014
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We aimed to evaluate the reliability and validity of the adapted Korean version of the Quality-of-Life Questionnaire of the European Foundation for Osteoporosis (QUALEFFO-26). Translation/retranslation of the English version of QUALEFFO was conducted, and all steps of the cross-cultural adaptation process were performed. The Korean version of the visual analog scale measure of pain, QUALEFFO-26 and the previously validated Short Form-36 (SF-36) were mailed to 162 consecutive patients with osteoporosis. Factor analysis and reliability assessment by kappa statistics of agreement for each item, the intraclass correlation coefficient and Cronbach's ? were conducted. Construct validity was also evaluated by comparing the responses of QUALEFFO-26 with the responses of SF-36 using Pearson's correlation coefficient. Factor analysis extracted 3 factors. All items had a kappa statistics of agreement greater than 0.6. The QUALEFFO-26 showed good test/retest reliability (QUALEFFO-26: 0.8271). Internal consistency of Cronbach's ? was found to be very good (QUALEFFO-26: 0.873). The Korean version of QUALEFFO-26 showed good significant correlation with SF-36 total score and with single SF-36 domains scores. The adapted Korean version of the QUALEFFO-26 was successfully translated and showed acceptable measurement properties and, as such, is considered suitable for outcome assessments in the Korean-speaking patients with osteoporosis.
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Microfluidic design of complex emulsions.
Chemphyschem
PUBLISHED: 01-09-2014
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Controllable generation of complex emulsions comprising exceptional features such as several compartments and shape anisotropy is becoming increasingly important. Complex emulsions are attracting great interest due to their significant potential in many applications, including foods, pharmaceuticals, cosmetics, materials, and chemical separations. Microfluidics is emerging as a promising route to the generation of complex emulsions, providing precise control over emulsion shape, size, and compartments. The aim of this Minireview is to mainly describe the progress of microfluidic approaches to design complex emulsions using hydrodynamic control and phase separation. The emulsions formed are classified according to their morphology, anisotropy, and internal structure. Emerging applications of complex emulsions formed using these microfluidic techniques are discussed.
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Non-thermal atmospheric pressure plasma induces apoptosis in oral cavity squamous cell carcinoma: Involvement of DNA-damage-triggering sub-G(1) arrest via the ATM/p53 pathway.
Arch. Biochem. Biophys.
PUBLISHED: 01-08-2014
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Recent advances in physics have made possible the use of non-thermal atmospheric pressure plasma (NTP) in cancer research. Although increasing evidence suggests that NTP induces death of various cancer cell types, thus offering a promising alternative treatment, the mechanism of its therapeutic effect is little understood. In this study, we report for the first time that NTP led to apoptotic cell death in oral cavity squamous cell carcinoma (OSCC). Interestingly, NTP induced a sub-G(1) arrest in p53 wild-type OSCCs, but not in p53-mutated OSCCs. In addition, NTP increased the expression levels of ATM, p53 (Ser 15, 20 and 46), p21, and cyclin D1. A comet assay, Western blotting and immunocytochemistry of ?H2AX suggested that NTP-induced apoptosis and sub-G(1) arrest were associated with DNA damage and the ATM/p53 signaling pathway in SCC25 cells. Moreover, ATM knockdown using siRNA attenuated the effect of NTP on cell death, sub-G(1) arrest and related signals. Taken together, these results indicate that NTP induced apoptotic cell death in p53 wild-type OSCCs through a novel mechanism involving DNA damage and triggering of sub-G(1) arrest via the ATM/p53 pathway. These findings show the therapeutic potential of NTP in OSCC.
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Survival of Skin Graft between Transgenic Cloned Dogs and Non-Transgenic Cloned Dogs.
PLoS ONE
PUBLISHED: 01-01-2014
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Whereas it has been assumed that genetically modified tissues or cells derived from somatic cell nuclear transfer (SCNT) should be accepted by a host of the same species, their immune compatibility has not been extensively explored. To identify acceptance of SCNT-derived cells or tissues, skin grafts were performed between cloned dogs that were identical except for their mitochondrial DNA (mtDNA) haplotypes and foreign gene. We showed here that differences in mtDNA haplotypes and genetic modification did not elicit immune responses in these dogs: 1) skin tissues from genetically-modified cloned dogs were successfully transplanted into genetically-modified cloned dogs with different mtDNA haplotype under three successive grafts over 63 days; and 2) non-transgenic cloned tissues were accepted into transgenic cloned syngeneic recipients with different mtDNA haplotypes and vice versa under two successive grafts over 63 days. In addition, expression of the inserted gene was maintained, being functional without eliciting graft rejection. In conclusion, these results show that transplanting genetically-modified tissues into normal, syngeneic or genetically-modified recipient dogs with different mtDNA haplotypes do not elicit skin graft rejection or affect expression of the inserted gene. Therefore, therapeutically valuable tissue derived from SCNT with genetic modification might be used safely in clinical applications for patients with diseased tissues.
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Non-thermal atmospheric pressure plasma inhibits thyroid papillary cancer cell invasion via cytoskeletal modulation, altered MMP-2/-9/uPA activity.
PLoS ONE
PUBLISHED: 01-01-2014
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Plasma, the fourth state of matter, is defined as a partially or completely ionized gas that includes a mixture of electrons and ions. Advances in plasma physics have made it possible to use non-thermal atmospheric pressure plasma (NTP) in cancer research. However, previous studies have focused mainly on apoptotic cancer cell death mediated by NTP as a potential cancer therapy. In this study, we investigated the effect of NTP on invasion or metastasis, as well as the mechanism by which plasma induces anti-migration and anti-invasion properties in human thyroid papillary cancer cell lines (BHP10-3 and TPC1). Wound healing, pull-down, and Transwell assays demonstrated that NTP reduced cell migration and invasion. In addition, NTP induced morphological changes and cytoskeletal rearrangements, as detected by scanning electron microscopy and immunocytochemistry. We also examined matrix metalloproteinase (MMP)-2/-9 and urokinase-type plasminogen activator (uPA) activity using gelatin zymography, uPA assays and RT-PCR. FAK, Src, and paxillin expression was detected using Western blot analyses and immunocytochemistry. NTP decreased FAK, Src, and paxillin expression as well as MMP/uPA activity. In conclusion, NTP inhibited the invasion and metastasis of BHP10-3 and TPC1 cells by decreasing MMP-2/-9 and uPA activities and rearranging the cytoskeleton, which is regulated by the FAK/Src complex. These findings suggest novel actions for NTP and may aid in the development of new therapeutic strategies for locally invasive and metastatic cancers.
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Epithelial?to?mesenchymal transition enhances the cardioprotective capacity of human amniotic epithelial cells.
Cell Transplant
PUBLISHED: 11-22-2013
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The amniotic epithelium consists of cells exhibiting mature epithelial cell characteristics but also varying degrees of stemness. We tested the hypothesis that induction of epithelial-tomesenchymal transition (EMT) in amniotic epithelial cells (AECs) derived from human placenta enhances their capacity to support the ischemic myocardium. In response to incubation with transforming growth factor-?1 protein (TGF-?1), AECs lost their cobblestone morphology and acquired a fibroblastoid shape, associated with downregulation of Ecadherin, upregulation of N-cadherin, Akt phosphorylation and intracellular periostin translocation. EMT-AECs displayed greatly enhanced mobility and secreted gelatinase activity as compared with naïve AECs. The surface presentation of CD105 and CD73 decreased, and RNA microarray analysis mirrored the loss of epithelial characteristics and transcriptional profile. Unmodified AECs and EMT-AECs were then injected intramyocardially in fully immunocompetent mice after permanent LAD ligation, and heart function was followed by MRI as well as 2Dspeckle tracking echocardiography after 4 weeks. EMT-AEC-treated infarct hearts displayed better global systolic function and improved longitudinal strain rate in the area-of-interest (AOI). Although no signals of human cells were detectable by histology, infarct size was smaller in EMT-AEC-treated hearts, associated with fewer TUNEL positive cells and upregulation of periostin, while blood vessel density was increased in both, AEC and EMT-AEC-treated hearts. We conclude that EMT enhances the cardioprotective effects of human AECs.
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Employing mated females as recipients for transfer of cloned dog embryos.
Reprod. Fertil. Dev.
PUBLISHED: 11-10-2013
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It has been suggested that co-transferring parthenogenetic embryos could improve the pregnancy success rate with cloned embryos in mammals. As an alternative to co-transferring parthenotes, in dogs we employed recipient females that possessed in vivo-fertilised embryos as a result of mating to determine whether mated bitches could be suitable recipients for cloned embryos. The effect of using mated recipients on implantation and pregnancy rates of canine somatic cell nuclear transfer embryos was also determined. Cloned embryos were transferred into the oviducts of naturally synchronous females that had mated with male dogs before ovulation. The pregnancy rate appeared to be similar between mated recipients (50%) and non-mated recipients (28.57%; P>0.05). However, the delivery rate of cloned pups was significantly higher in mated recipients than non-mated recipients (10.53 vs 2.38%; P<0.05). A decrease in progesterone levels in the mated recipients before the due date induced natural delivery. However, cloned pups in non-mated recipients were delivered by Caesarean section because the fall in progesterone concentration in these females did not occur until the due date. The present study demonstrated for the first time that mated female dogs can be used as recipients for cloned embryos.
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Prematurational Culture with IBMX Synchronizes Meiotic Progression of the Germinal Vesicle Stage and Improves Nuclear Maturation and Embryonic Development in In Vitro-grown Bovine Oocytes.
J. Reprod. Dev.
PUBLISHED: 11-09-2013
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The objective of this study was to clarify the effects of prematurational culture (pre-IVM) supplemented with 3-isobutyl-1-methylxanthine (IBMX) on nuclear and cytoplasmic maturation of in vitro-grown bovine oocytes. In experiment 1, oocytes (95 ?m in diameter) derived from early antral follicles (0.5-1?mm in diameter) were cultured for 12days for in vitro growth (IVG). IVG oocytes with a normal appearance were subjected to examinations of diameter and chromatin structure in the germinal vesicle (GV) before IVM. In addition, percentages of metaphase II (M II) were examined after IVM. Regardless of pre-IVM, the mean diameters of IVG oocytes were about 115 ?m. The proportions of GV3 (50.0%) and M II stages (80.1%) of IVG oocytes with pre-IVM were higher than those without pre-IVM (28.0 and 49.4%, respectively). In experiment 2, the fertilizability and developmental competence of IVG oocytes were examined. Regardless of pre-IVM, the normal fertilization rates of IVG oocytes were similar (around 70%) but were lower than that of in vivo-grown oocytes (88.0%). Cleavage and blastocyst rates of IVG oocytes with pre-IVM (63.0 and 26.1%, respectively) were higher than those without pre-IVM (45.8 and 12.7%, respectively). The blastocyst rate based on cleaved IVG oocytes with pre-IVM (41.7%) was similar to that of in vivo-grown oocytes (48.7%), although the cleavage rate of IVG oocytes with pre-IVM was lower than that of in vivo-grown oocytes. In conclusion, pre-IVM with IBMX improved the maturational and developmental competences of IVG oocytes, probably due to promotion of their chromatin transition and synchronization of meiotic progression.
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Effects of art therapy using color on purpose in life in patients with stroke and their caregivers.
Yonsei Med. J.
PUBLISHED: 10-30-2013
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Patients with stroke suffer from physical disabilities, followed by mental instability. Their caregivers also suffer from mental instability. The present study attempted to address the degree and the change of the level of Purpose in Life (PIL) in patients with stroke and caregivers by applying art therapy using colors.
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Different Zn(II) cation coordination geometries in di-?-acetato-bis{2-chloro-6-[(pyridine-2-ylmethylimino)methyl]phenol}dizinc(II) chloroform monosolvate.
Acta Crystallogr C
PUBLISHED: 08-17-2013
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In the title compound, di-?-acetato-?(2)O:O;?(2)O:O-bis[(6-chloro-2-{(E)-[(pyridin-2-yl)methylimino]methyl}phenolato-?(3)N,N,O)zinc(II)], [Zn2(C13H10ClN2O)2(C2H3O2)2]·CHCl3, the Zn(II) cation adopts a five-coordinate geometry and is coordinated by two N atoms and one O atom of a tridentate 6-chloro-2-{(E)-[(pyridin-2-yl)methylimino]methyl}phenolate ligand and two O atoms of two bridging acetate groups, but their coordination geometries differ. One Zn(II) cation adopts a distorted trigonal bipyramidal geometry and the other a square-pyramidal geometry. The two acetate ligands bridge two Zn(II) cations with mono- and bidentate coordination modes. The title compound exhibits a strong emission at 460?nm upon excitation at 325?nm with a quantum yield of 23.1%.
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Radioprotective effect of epicatechin in cultured human fibroblasts and zebrafish.
J. Radiat. Res.
PUBLISHED: 08-16-2013
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Radiation-induced normal cell damage limits the delivery of high-dose radiation to targeted cancer. This study investigated the effect of epicatechin (EC), a minor component of green tea extracts, on radiation-induced cellular damage in vitro in primary cultured human fibroblasts and in vivo in a zebrafish model. Cell viability, proliferation and wound-healing efficacy, mitochondrial membrane potential, and reactive oxygen species (ROS) generation as well as changes in the signaling pathway related to apoptosis were investigated in fibroblasts. The therapeutic effects of EC were explored in a zebrafish model. EC increased clonogenic survival and restored the migration ability of the fibroblasts after irradiation. EC inhibited radiation-induced ROS generation, mitochondrial dysfunction and cell death. EC significantly reduced the expression of p-JNK, p-38, and cleaved caspase-3 compared with their significant increase after radiation treatment. EC attenuated the radiation-induced embryotoxicity in a zebrafish model. These results suggest that EC represents an effective means of reducing cellular damage and facilitating wound healing after radiation exposure.
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Effects of Xanthium stramarium and Psoralea corylifolia Extracts Combined with UVA1 Irradiation on the Cell Proliferation and TGF-?1 Expression of Keloid Fibroblasts.
Ann Dermatol
PUBLISHED: 08-13-2013
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Xanthium stramarium (XAS) and Psoralea corylifolia (PSC), phototoxic oriental medicinal plants, has been used in traditional medicines in Asian countries.
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1-Benzoyl-4-thio-biuret.
Acta Crystallogr Sect E Struct Rep Online
PUBLISHED: 08-01-2013
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{[(phenyl-formamido)-carbon-yl]amino}-methane-thio-amide), C9H9N3O2S, both benzoyl and terminal thio-urea fragments adopt transoid conformations with respect to the central carbonyl O atom. The benzoyl and thio-biuret groups are almost coplanar, making a dihedral angle of 4.40?(8)°. The mol-ecular structure is stabilized by two intra-molecular N-H?O hydrogen bonds. In the crystal, N-H?O and N-H?S hydrogen bonds link the mol-ecules into a tape running along [101].
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Bis[2-(1,3-benzo-thia-zol-2-yl)phenyl-?(2) C (1),N][1,3-bis-(4-bromo-phen-yl)propane-1,3-dionato-?(2) O,O]iridium(III).
Acta Crystallogr Sect E Struct Rep Online
PUBLISHED: 08-01-2013
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The title complex, [Ir(C15H9Br2O2)(C13H8NS)2], lies about a crystallographic twofold rotation axis passing through the Ir(III) atom and the central C atom of the bis-(bromo-phen-yl)propane-1,3-dionate ligand. The Ir(III) atom adopts a distorted octa-hedral geometry coordinated by two N atoms in the axial positions, and two C and two O atoms in the equatorial plane. The dihedral angle between the two thia-zole ring systems in the complex is 77.45?(10)°.
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Synthesis of mononuclear copper(II) complexes of acyclic Schiffs base ligands: Spectral, structural, electrochemical, antibacterial, DNA binding and cleavage activity.
Spectrochim Acta A Mol Biomol Spectrosc
PUBLISHED: 07-29-2013
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The mononuclear copper(II) complexes (1&2) of ligands L(1) [N,N-bis(2-hydroxy-5-methylbenzyl)-1,4-bis(3-iminopropyl)piperazine] or L(2) [N,N-bis(2-hydroxy-5-bromobenzyl)-1,4-bis(3-iminopropyl) piperazine] have been synthesized and characterised. The single crystal X-ray study had shown that ligands L(1) and L(2) crystallize in a monoclinic crystal system with P21/c space group. The mononuclear copper(II) complexes show one quasireversible cyclic voltammetric response near cathodic region (-0.77 to -0.85V) in DMF assignable to the Cu(II)/Cu(I) couple. Binding interaction of the complexes with calf thymus DNA (CT DNA) investigated by absorption studies and fluorescence spectral studies show good binding affinity to CT DNA, which imply both the copper(II) complexes can strongly interact with DNA efficiently. The copper(II) complexes showed efficient oxidative cleavage of plasmid pBR322 DNA in the presence of 3-mercaptopropionic acid as reducing agent through a mechanistic pathway involving formation of singlet oxygen as the reactive species. The Schiff bases and their Cu(II) complexes have been screened for antibacterial activities which indicates that the complexes exhibited higher antimicrobial activity than the free ligands.
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Spontaneous recanalization of an occluded middle cerebral artery in a patient with moyamoya.
Vasc Endovascular Surg
PUBLISHED: 07-12-2013
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Moyamoya disease is a rare cerebrovascular disorder that is characterized by progressive stenosis of the distal portion of the internal carotid artery and its main branches. Here the authors present the first case of spontaneous recanalization of an occluded middle cerebral artery in a woman with moyamoya who had not undergone surgical or endovascular treatment. Although the exact mechanisms remain unclear, spontaneous recanalization in moyamoya could be considered a possible phenomenon. Thus, the neurosurgeons should always pay attention to the pathological variety of dynamic changes in the cerebrovascular structure in moyamoya.
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Associations of serum fetuin-A levels with insulin resistance and vascular complications in patients with type 2 diabetes.
Diab Vasc Dis Res
PUBLISHED: 06-27-2013
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We examined the relationship between serum fetuin-A, insulin resistance (IR), metabolic syndrome (MS) and vascular complications including cardiac autonomic neuropathy (CAN) in patients with type 2 diabetes mellitus (T2DM).
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Controllable preparation of monodisperse microspheres using geometrically mediated droplet formation in a single mold.
Langmuir
PUBLISHED: 06-27-2013
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We present a surfactant-free fabrication method for simultaneous generation of monodisperse microspheres with controllable size manner. Droplets that become microspheres by solidification processes are made in a two-step process: capillary rising-induced fluid division and wetting of immiscible fluid in a micromold. Design of the mold geometry and the monomer concentration primarily determines the microsphere size and the size distribution. Furthermore, the synergistic effect of two parameters is able to efficiently manipulate the microsphere sizes from submicrometers to a few hundred micrometers.
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