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Find video protocols related to scientific articles indexed in Pubmed.
A low-power photonic quantization approach using OFDM subcarrier spectral shifts.
Opt Express
PUBLISHED: 11-18-2014
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Photonic analog-to-digital conversion and optical quantization are demonstrated, based on the spectral shifts of orthogonal frequency division multiplexing subcarriers and a frequency-packed arrayed waveguide grating. The system is extremely low-energy consuming since the spectral shifts are small and generated by cross-phase modulation, using a linear-slope high-speed and low-jitter pulse train generated by a mode locked laser diode. The feasibility of a 2, 3 and 4-bit optical quantization scheme is demonstrated.
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First demonstration of a scalable MDM/CDM optical access system.
Opt Express
PUBLISHED: 06-13-2014
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A novel hybrid all-optical mode-division multiplexing and code division multiplexing architecture for flexible and scalable access networks is presented. We successfully demonstrate, for the first time, an asynchronous on-off keying modulation, 2 mode x 4 code x 10 Gbps transmission over 42-km link, using a set of single-mode and two-mode fibers, without dispersion compensation. The four phase-shift keyed optical codes are generated at a single wavelength, by a multiport encoder/decoder, and we use an optical mode multiplexer/demultiplexer in the remote node and at the central office. We also experimentally evaluate the mode crosstalk tolerance considering different access span distances for the LP(01) and LP(11) modes.
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Induction of AhR-mediated gene transcription by coffee.
PLoS ONE
PUBLISHED: 01-01-2014
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Aryl hydrocarbon receptor (AhR) is classically known to be activated by xenobiotics such as dioxins and polycyclic aromatic hydrocarbons (PAHs). Although it has been reported that PAHs are contained in roasted coffee beans, in general coffee beverages are not considered to be AhR activators. We tested whether exposure to coffee would activate AhR in cultured cells.
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Identification of compounds with potential antibacterial activity against Mycobacterium through structure-based drug screening.
J Chem Inf Model
PUBLISHED: 04-19-2013
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To identify novel antibiotics against Mycobacterium tuberculosis, we performed a hierarchical structure-based drug screening (SBDS) targeting the enoyl-acyl carrier protein reductase (InhA) with a compound library of 154,118 chemicals. We then evaluated whether the candidate hit compounds exhibited inhibitory effects on the growth of two model mycobacterial strains: Mycobacterium smegmatis and Mycobacterium vanbaalenii. Two compounds (KE3 and KE4) showed potent inhibitory effects against both model mycobacterial strains. In addition, we rescreened KE4 analogs, which were identified from a compound library of 461,383 chemicals through fingerprint analysis and genetic algorithm-based docking simulations. All of the KE4 analogs (KES1-KES5) exhibited inhibitory effects on the growth of M. smegmatis and/or M. vanbaalenii. Based on the predicted binding modes, we probed the structure-activity relationships of KE4 and its analogs and found a correlative relationship between the IC50 values and the interaction residues/LogP values. The most potent inhibitor, compound KES4, strongly and stably inhibited the long-term growth of the model bacteria and showed higher inhibitory effects (IC50 = 4.8 ?M) than isoniazid (IC50 = 5.4 ?M), which is a first-line drug for tuberculosis therapy. Moreover, compound KES4 did not exhibit any toxic effects that impede cell growth in several mammalian cell lines and enterobacteria. The structural and experimental information of these novel chemical compounds will likely be useful for the development of new anti-TB drugs. Furthermore, the methodology that was used for the identification of the effective chemical compound is also likely to be effective in the SBDS of other candidate medicinal drugs.
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Visible light photocatalysis with c-WO(3-x)/WO3×H2O nanoheterostructures in situ formed in mesoporous polycarbosilane-siloxane polymer.
J. Am. Chem. Soc.
PUBLISHED: 03-11-2013
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In recent years, there have been significant efforts to find novel photocatalytic materials with improved properties. Thus, there is an active ongoing search for new materials that can operate at a broad range of wavelengths for photocatalytic reactions. Among photocatalytically active semiconductors, considerable attention has been given to tungsten oxide with a band gap of E(g) ? 2.6 eV, which provides the opportunity to harvest visible light. In the present work, we report on a one-step synthesis of c-WO(3-x)/WO3×H2O nanowhiskers dispersed in a hydrolytically stable mesoporous polycarbosilane-siloxane ([-Si(O)CH2-]n) matrix. The as-synthesized nanocomposites possess high photocatalytic activity for the degradation of methylene blue (MB) under visible light irradiation. The enhanced photocatalytic activity is due to (i) the reduction in the electron-hole recombination rate because of the reduced dimensions of nanowhiskers, (ii) more efficient consumption of photogenerated electrons and holes as a result of the high surface-to-bulk-ratio of the nanowhiskers, and (iii) better electron-hole pair separation due to the formation of c-WO(3-x)/WO3×H2O nanoheterostructures.
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The influence of fixation in formalin on the measurement of stability of implants using resonance frequency analysis and Periotest M(®): A study in a dog.
Br J Oral Maxillofac Surg
PUBLISHED: 02-13-2013
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Resonance frequency analysis (RFA) and Periotest(®) have been widely used to evaluate measurements of the stability of implants in clinical studies and animal experiments. In animals, these measurements are often made after bone has been fixed in formalin. However, it is not yet clear how this fixation influences RFA and Periotest(®), and our aim was to clarify this using the implant stability quotient (ISQ) and Periotest(®) value (PTV). Six titanium implants were placed, 3 into each femur, of one male dog (a beagle/labrador cross). After 2 months blocks of bone were harvested, each block containing 3 implants, and were fixed in 10% neutral formalin. Measurements were made before fixation (time 0) then at 4h, and 1, 7, 14, 21, and 28 days. ISQ values of 6 implants were evaluated 3 times on the short and long axes, as were PTV. ISQ ranged from 67.5 to 79.3 and tended to increase with time. ISQ at 7-28 days were significantly higher than that at time 0. PTV ranged from -7.7 to -7.8 and did not differ significantly among fixation times. These results suggest that fixation of bony tissue in formalin might affect ISQ, so it might be preferable to measure ISQ during the early stages of fixation.
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Inorganic polyphosphate suppresses lipopolysaccharide-induced inducible nitric oxide synthase (iNOS) expression in macrophages.
PLoS ONE
PUBLISHED: 01-01-2013
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In response to infection, macrophages produce a series of inflammatory mediators, including nitric oxide (NO), to eliminate pathogens. The production of these molecules is tightly regulated via various mechanisms, as excessive responses are often detrimental to host tissues. Here, we report that inorganic polyphosphate [poly(P)], a linear polymer of orthophosphate ubiquitously found in mammalian cells, suppresses inducible nitric oxide synthase (iNOS) expression induced by lipopolysaccharide (LPS), a cell wall component of Gram-negative bacteria, in mouse peritoneal macrophages. Poly(P) with longer chains is more potent than those with shorter chains in suppressing LPS-induced iNOS expression. In addition, poly(P) decreased LPS-induced NO release. Moreover, poly(P) suppressed iNOS mRNA expression induced by LPS stimulation, thereby indicating that poly(P) reduces LPS-induced iNOS expression by down-regulation at the mRNA level. In contrast, poly(P) did not affect the LPS-induced release of TNF, another inflammatory mediator. Poly(P) may serve as a regulatory factor of innate immunity by modulating iNOS expression in macrophages.
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Comparative evaluation of bone regeneration using spherical and irregularly shaped granules of interconnected porous hydroxylapatite. A beagle dog study.
J Prosthodont Res
PUBLISHED: 02-09-2011
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Bone regeneration stimulated by two different shapes of interconnected porous calcium hydroxyapatite (IP-CHA) granules was evaluated in the mandibles of 3 beagle dogs.
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Inflammation-based prognostic score and number of lymph node metastases are independent prognostic factors in esophageal squamous cell carcinoma.
Dig Surg
PUBLISHED: 06-22-2010
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Few studies have investigated whether the Glasgow Prognostic Score (GPS), an inflammation-based prognostic score, is useful for postoperative prognosis of esophageal squamous cell carcinoma.
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Elevated C-reactive protein and hypoalbuminemia measured before resection of colorectal liver metastases predict postoperative survival.
Dig Surg
PUBLISHED: 01-17-2010
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Few studies have investigated whether the Glasgow Prognostic Score (GPS), an inflammation-based prognostic score measured before resection of colorectal liver metastasis (CRLM), can predict postoperative survival.
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The influence of endotoxemia on the electroencephalographic and antinociceptive effects of isoflurane in a swine model.
Anesth. Analg.
PUBLISHED: 11-06-2009
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We have previously reported that hemorrhagic shock decreases the minimum alveolar anesthetic concentration (MAC) of isoflurane but minimally alters the electroencephalographic (EEG) effect. In this study, we investigated the influence of endotoxemia on the EEG effect and the MAC of isoflurane.
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The influence of hemorrhagic shock on the electroencephalographic and immobilizing effects of propofol in a swine model.
Anesth. Analg.
PUBLISHED: 07-18-2009
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Hemorrhagic shock increases the hypnotic effect of propofol, but the influence of hemorrhagic shock on the immobilizing effect of propofol is not fully defined.
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Initial bone regeneration around fenestrated implants in Beagle dogs using basic fibroblast growth factor-gelatin hydrogel complex with varying biodegradation rates.
J Prosthodont Res
PUBLISHED: 03-26-2009
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The purpose of this study was to compare the effectiveness of fast and slow biodegradation of basic fibroblast growth factor (bFGF)-gelatin hydrogel complex on bone regeneration around fenestrated implants as a new augmentation drug delivery system.
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Evidence that PKA activity is constitutively activated in human GH-secreting adenoma cells in a patient with Carney complex harbouring a PRKAR1A mutation.
Clin. Endocrinol. (Oxf)
PUBLISHED: 01-31-2009
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The GHRH-protein kinase A (PKA) signalling pathway is essential for cell proliferation and GH synthesis/secretion in somatotrophs. An inactivating mutation of PRKAR1A is one of the causes of somatotrophinoma in Carney complex (CNC). The basal PKA activity of somatotroph adenoma cells from CNC has not been evaluated because of a limited amount of available tissue.
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Development of implant/interconnected porous hydroxyapatite complex as new concept graft material.
PLoS ONE
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Dental implant has been successfully used to replace missing teeth. However, in some clinical situations, implant placement may be difficult because of a large bone defect. We designed novel complex biomaterial to simultaneously restore bone and place implant. This complex was incorporated implant into interconnected porous calcium hydroxyapatite (IP-CHA). We then tested this Implant/IP-CHA complex and evaluated its effect on subsequent bone regeneration and implant stability in vivo.
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Inorganic polyphosphates stimulate FGF23 expression through the FGFR pathway.
Biochem. Biophys. Res. Commun.
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Polyphosphate (polyP) is composed of linear polymers of orthophosphate residues linked by high-energy phosphoanhydride bonds. It has been reported to improve osteoblastic differentiation, stimulate periodontal tissue regeneration, and accelerate bone repair. The aim of this study was to evaluate the effect of polyP on the expression of FGF23, a hormone secreted mostly be mature osteoblasts and osteocytes. In this study, different types of polyP were synthesized and co-cultured with osteoblast-like UMR-106 cells. Real-time PCR and western blot were used to analyze the gene and protein expression of FGF23. We found that 1 mM polyP was able to increase FGF23 expression after 4 h, reaching a peak after 12-24 h, with expression decreasing by 48 h. We also found that polyP could activate the FGFR pathway, as evidenced by increased phosphorylation of FGFR, FRS2, and Erk1/2. When FGFR signaling was inhibited by the specific inhibitor SU5402, the effect of polyP on FGF23 expression was significantly reduced. Our results indicate that polyP is able to stimulate osteoblastic FGF23 expression and that this effect is associated with activation of the FGFR pathway. These findings provide support for the clinical use of polyP by indicating a mechanism for polyP in bone regeneration.
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Influence of formalin fixation on the implant stability quotient and mechanical characteristics of bone.
Br J Oral Maxillofac Surg
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The implant stability quotient (ISQ) has been widely evaluated in clinical studies and animal experiments. However, accurate measurement is often difficult in animal models. In such cases, measurement of ISQ in bone is needed after formalin fixation. However, it is not yet clear how such fixation influences the measurement. The purpose of this study was to investigate the influence of formalin fixation on ISQ and the mechanical characteristics of bone. Fourteen tibias were harvested from rabbits; the samples from the left (length 60mm, control group) were soaked in saline and the samples from the right (length 60mm, fixation group) were fixed in 10% neutral-buffered formalin at 4°C for 4h. Three-point bending tests were done at 5mm/min to measure the maximum load and total absorbed energy. Twelve titanium implants (Brånemark System(®) Mk-III TiUnite, Nobel Biocare, Sweden) were placed into the edentulous molar site of the mandibles of 2 dogs and the ISQ was measured by Osstell(®) (control group) 3 months later. The implants involved in the bone block were then fixed for 4h (fixation group) and the ISQ measured. The maximum load values did not differ significantly between the control and fixation groups. Total absorbed energy was significantly higher in the control group than in the fixation group. ISQ did not differ significantly between the groups. These results suggest that formalin fixation of bone might affect some of the mechanical characteristics of bone, but not its ISQ.
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Biobased polymers: synthesis of graft copolymers and comb polymers using lactic acid macromonomer and properties of the product polymers.
Biomacromolecules
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For developing broader application of biobased polymers, graft copolymers and comb polymers having poly(lactic acid) (PLA) side chains have been synthesized by using a macromonomer technique. PLA macromonomers (MMm) having a methacryloyl polymerizable group with different PLA chain length with an average length m = 4, 6, 8, 12, 18, and 30 were prepared via ring-opening polymerization of l-lactide using hydroxyethyl methacrylate (HEMA) initiator catalyzed by Sn(Oct)(2). Radical polymerization behaviors of these macromonomers were examined. Radical copolymerization of MMm (m = 4, 6, and 8), with vinyl monomers like n-butyl methacrylate (BMA) and n-butyl acrylate (BA) in water as the reaction medium, gave stable miniemulsions of poly[n-butyl (meth)acrylate-graft-lactic acid]s [PB(M)A-g-PLAm]. MMm with m value higher than 12, however, gave aggregate products in a minor amount besides miniemulsions in a major amount, producing not a stable emulsion system of graft copolymers. The solution copolymerization, on the other hand, produced a wider variety of the graft copolymers, where a wider range of MMm (even m ? 12) can be employed. In a 1,4-dioxane solution, the radical copolymerization of MMm with BMA and methyl methacrylate (MMA) gave various graft copolymers [PB(M)MA-g-PLAm]. A new type of comb polymers (PMMm) having PLAm as pendant side chains were obtained by radical homopolymerization of MMm in a 1,4-dioxane solution. The graft copolymers and comb polymers obtained here are amorphous. Physical properties of the polymers from miniemulsions suggested them to be applicable for coatings or elastic materials which are environmentally desirable as a new class of biobased polymers. In addition, the present approach provided fundamental information on relationships between the length of PLA side chain and the bulk properties of the product polymers.
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Influence of progressive hemorrhage and subsequent cardiopulmonary resuscitation on the bispectral index during isoflurane anesthesia in a swine model.
J Trauma Acute Care Surg
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The bispectral index for measurement of anesthetic depth may be modified by extreme hypotension during hemorrhagic shock. In this study, the influence of progressive hemorrhage and subsequent cardiopulmonary resuscitation on the bispectral index was investigated under controlled anesthetic depth.
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Cross-clamping of the descending thoracic aorta leads to the asymmetrical distribution of propofol during cardiopulmonary bypass surgery.
Korean J Anesthesiol
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We hypothesized that cross-clamping of the descending thoracic aorta (CcDTA) would result in significant changes in plasma propofol concentrations (Cp) proximal and distal to the cross-clamp. We investigated the effect of CcDTA on Cp centrally and distally, including the pulmonary artery and the cardiopulmonary bypass (CPB) cannula.
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What is Visualize?

JoVE Visualize is a tool created to match the last 5 years of PubMed publications to methods in JoVE's video library.

How does it work?

We use abstracts found on PubMed and match them to JoVE videos to create a list of 10 to 30 related methods videos.

Video X seems to be unrelated to Abstract Y...

In developing our video relationships, we compare around 5 million PubMed articles to our library of over 4,500 methods videos. In some cases the language used in the PubMed abstracts makes matching that content to a JoVE video difficult. In other cases, there happens not to be any content in our video library that is relevant to the topic of a given abstract. In these cases, our algorithms are trying their best to display videos with relevant content, which can sometimes result in matched videos with only a slight relation.