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Find video protocols related to scientific articles indexed in Pubmed.
Multidimensional analysis of gene expression reveals TGFB1I1-induced EMT contributes to malignant progression of astrocytomas.
Oncotarget
PUBLISHED: 10-22-2014
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Malignant progression of astrocytoma is a multistep process with the integration of genetic abnormalities including grade progression and subtypes transition. Established biomarkers of astrocytomas, like IDH1 and TP53 mutation, were not associated with malignant progression. To identify new biomarker(s) contributing to malignant progression, we collected 252 samples with whole genome mRNA expression profile [34 normal brain tissue (NBT), 136 grade II astrocytoma (AII) and 82 grade III astrocytoma (AIII)]. Bioinformatics analysis revealed that EMT-associated pathways were most significantly altered along with tumor grades progress with up-regulation of 17 genes. Up-regulation of these genes was further confirmed by RNA-sequencing in 128 samples. Survival analysis revealed that high expression of these genes indicates a poor survival outcome. We focused on TGFB1I1 (TGF-?1 induced transcript 1) whose expression correlation with WHO grades was further validated by qPCR in 6 cell lines of different grades and 49 independent samples (36 AIIs and 13 AIIIs). High expression of TGFB1I1 was found associated with subtype transition and EMT pathways activation. The conclusion was confirmed using immunohistochemistry in tissue microarrays. Studies in vitro and vivo using TGF-?1 and TGFB1I1 shRNA demonstrated that TGFB1I1 is required for TGF-? stimulated EMT that contributes to malignant progression of astrocytomas.
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Maturation of glutamatergic transmission in the vestibulo-olivary pathway impacts on the registration of head rotational signals in the brainstem of rats.
Brain Struct Funct
PUBLISHED: 09-23-2014
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The recognition of head orientation in the adult involves multi-level integration of inputs within the central vestibular circuitry. How the different inputs are recruited during postnatal development remains unclear. We hypothesize that glutamatergic transmission at the vestibular nucleus contributes to developmental registration of head orientations along the vestibulo-olivary pathway. To investigate the maturation profile by which head rotational signals are registered in the brainstem, we used sinusoidal rotations on the orthogonal planes of the three pairs of semicircular canals. Fos expression was used as readout of neurons responsive to the rotational stimulus. Neurons in the vestibular nucleus and prepositus hypoglossal nucleus responded to all rotations as early as P4 and reached adult numbers by P21. In the reticular formation and inferior olive, neurons also responded to horizontal rotations as early as P4 but to vertical rotations not until P21 and P25, respectively. Neuronal subpopulations that distinguish between rotations activating the orthogonally oriented vertical canals were identifiable in the medial and spinal vestibular nuclei by P14 and in the inferior olivary subnuclei IO? and IOK by P25. Neonatal perturbation of glutamate transmission in the vestibular nucleus was sufficient to derange formation of this distribution in the inferior olive. This is the first demonstration that developmental refinement of glutamatergic synapses in the central vestibular circuitry is essential for developmental registration of head rotational signals in the brainstem.
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Photoelectrochemical batteries for efficient energy recovery.
Chem. Commun. (Camb.)
PUBLISHED: 09-18-2014
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Herein we propose novel photoelectrochemical fuel cells (PEFCs) by the introduction of a solid-state Ag2O/Ag cathode (here also term as photoelectrochemical battery). Due to the superior electrochemical properties of Ag2O/Ag, the maximum power density of our PEFCs can reach 0.94 mW cm(-2) upon UV illumination. Furthermore, our PEFCs have stable cycle operation and can be undertaken in a single chamber without an ion-exchange membrane. Most importantly, we demonstrate that our PEFCs can be adopted to degrade the methyl orange (MO) dye with a decomposition percentage of 72.5% within 30 min during the PEFC process.
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Neurotensin, a Novel Messenger to Cross-Link Inflammation and Tumor Invasion via Epithelial-Mesenchymal Transition Pathway.
Int. Rev. Immunol.
PUBLISHED: 09-13-2014
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Multiple cytokines and growth factors are critical for the prognosis of cancer which has been regarded as a worldwide health problem. Recently, neuropeptides, soluble factors regulating a series of functions in the central nervous system, have also been demonstrated to stimulate the proliferation and migration of tumor cells. Among these signaling peptides, the role of neurotensin (NTS) on malignancy procession has become a hot topic. The effects of NTS on tumor growth and its antiapoptosis role have already been identified. Subsequently, studies demonstrated the impact of NTS on the migration and invasion, but the molecular mechanisms involved are still unclear at present. Recently, some reports indicated that NTS could induce expression and secretion of interleukin-8 (IL-8) to promote local imflammatory response which might participate in epithelial-mesenchymal transition (EMT)-related tumor migration. In present review, we highlight the process of tumor EMT induced by NTS through stimulating IL-8 and the significance of NTS/IL-8 pathway in clinical application prospect.
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[Treatment of thoracolumbar fractures with unilateral pedicle screw fixation through paraspinal approach].
Zhongguo Gu Shang
PUBLISHED: 08-30-2014
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To evaluate the feasibility and efficacy of unilateral pedicle screw fixation in treating thoracolumbar fractures through paraspinal approach.
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Polymorphisms in SPARC and coal workers' pneumoconiosis risk in a Chinese population.
PLoS ONE
PUBLISHED: 08-15-2014
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The SPARC is a crucial matricellular protein and may influence the course of various diseases like tumor metastasis and fibrosis. In the present study, we investigated the association between the potential functional polymorphisms in SPARC and coal workers' pneumoconiosis (CWP) risk in a Chinese population.
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Expression and purification of non-tagged recombinant mouse SPP1 in E. coli and its biological significance.
Bioengineered
PUBLISHED: 08-12-2014
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Secreted phosphoprotein 1 (SPP1) is a multifunctional protein expressed by cells from a large variety of tissues. It is involved in many physiological and pathological processes, including bone metabolism, inflammation progress, tumor metastasis, injury repair, hyperoxia-induced injury, and so on. Native SPP1 from multiple species have been isolated from the milk and urine, and recombinant SPP1 with different tags have been expressed and purified from bacteria. In our study, DNA fragments corresponding to mouse SPP1 without signal peptide were built into the pET28a(+) vector, and non-tagged recombinant mouse SPP1(rmSPP1) was expressed in Escherichia coli BL21(DE3). rmSPP1 was purified using a novel tri-step procedure, and the product features high purity and low endotoxin level. rmSPP1 can effectively increase hepatocellular carcinoma cell(HCC) proliferation in vitro, demonstrating its biological activity.
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Soluble a?proliferation?inducing ligand (sAPRIL), a novel serum biomarker predicting the recurrence and metastasis of pancreatic adenocarcinoma after surgery.
Mol Med Rep
PUBLISHED: 08-04-2014
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Pancreatic adenocarcinoma (PA) is a leading cause of adult cancer mortality, and surgery is still the best available treatment strategy. However, PA can recur at any time and has limited prognosis. It is therefore necessary to explore novel serum biomarkers of PA to allow the early diagnosis of PA. Soluble a-proliferation-inducing ligand (sAPRIL), a promising inducer of the epithelial-mesenchymal transition (EMT), is often found overexpressed in a variety of autoimmune diseases. To determine whether serum sAPRIL can constitute a PA biomarker, the protein level of sAPRIL was examined by immunohistochemistry and western blot, and the mRNA level was quantified by RT-qPCR. The PA cell line PanC-1 was transfected with vectors bearing the sAPRIL gene and sAPRIL short hairpin RNA (shRNA) oligos. Increased expression of serum sAPRIL was observed in patients with PA recurrence or metastasis after five-year surgery compared to subjects without PA recurrence or metastasis. The growth rate of PanC-1 cells transfected with the sAPRIL expression vector was increased by 23% (P<0.01, vs. control group), and was reduced by 17% (P<0.01, vs. control group) in the sAPRIL shRNA-silenced cell line. Thus, sAPRIL is highly expressed in PA, and serum levels of sAPRIL can serve as a useful indicator for the recurrence or metastasis of PA after surgery. Additional validation studies on the use of serum sAPRIL as a diagnostic marker in PA are however needed.
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The E3 deubiquitinase USP17 is a positive regulator of retinoic acid-related orphan nuclear receptor ?t (ROR?t) in Th17 cells.
J. Biol. Chem.
PUBLISHED: 07-28-2014
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Stable retinoic acid-related orphan nuclear receptor ?t (ROR?t) expression is pivotal for the development and function of Th17 cells. Here we demonstrate that expression of the transcription factor ROR?t can be regulated through deubiquitination, which prevents proteasome-mediated degradation. We establish that USP17 stabilizes ROR?t protein expression by reducing ROR?t polyubiquitination at its Lys-360 residue. In contrast, knockdown of endogenous USP17 in Th17 cells resulted in decreased ROR?t protein levels and down-regulation of Th17-related genes. Furthermore, USP17 expression was up-regulated in CD4(+) T cells from systemic lupus erythematosus patients. Our data reveal a molecular mechanism in which ROR?t expression in Th17 cells can be positively regulated by USP17, thereby modulating Th17 cell functions.
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Intestinal pork tapeworm disease mimicking lymphoma on PET/CT imaging.
Clin Nucl Med
PUBLISHED: 07-08-2014
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A 17-year-old girl with abdominal pain for 3 months underwent 18F-FDG PET to evaluate possible malignancy due to unexplained ascites detected by ultrasound. The images demonstrated multiple foci of abnormally increased 18F-FDG activity in the lymphadenopathy from the neck to the abdomen and in the thickened intestinal wall. In addition, abnormal activity was noted in the pharynx and skeletons. Cysticercosis was confirmed pathologically.
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Combination of Apolipoprotein A1-Modi Liposome-Doxorubicin with Autophagy Inhibitors Overcame Drug Resistance In Vitro.
J Pharm Sci
PUBLISHED: 06-27-2014
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Multidrug resistance (MDR) represents the major drawback in chemotherapy. Liposome-based approaches could reverse MDR to some extent through circumventing the active efflux effect of P-glycoprotein. However, the reverse power of liposome is very limited because the nontargeting liposome is inefficient to deliver drugs to tumor actively. Besides, autophagy could reinforce the resistance of tumor cells to the cytotoxicity of intracellular drugs. Here, liposomal doxorubicin (Lipodox) that was conjugated with apolipoprotein A1-apo-Lipodox, was employed in tumor targeting delivery of doxorubicin. In the experiments, apo-Lipodox could enter cells effectively and reverse MDR more efficiently than their nontargeting counterpart. Autophagy occurred in this process and contributed to the survival of tumor cells. Combination use of autophagy inhibitors could enhance the cytotoxicity of apo-Lipodox and reverse drug resistance to a higher degree. We propose that this strategy holds promise to overcome MDR in human cancer. © 2014 Wiley Periodicals, Inc. and the American Pharmacists Association J Pharm Sci 103:3994-4004, 2014.
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Selective aerobic oxidation of alcohols to aldehydes, carboxylic acids, and imines catalyzed by a Ag-NHC complex.
Org. Lett.
PUBLISHED: 06-17-2014
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Silver NHC catalysts have been developed for the selective oxidation of alcohols to aldehydes or carboxylic acids in the presence of BnMe3NOH or KOH under dry air. The aerobic oxidation conditions are mild, and the yield is excellent. Further tandem catalysis enables the one-pot synthesis of imines in excellent yield. Only 0.1 mol % of the catalyst is required.
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Identification of a novel MTOR activator and discovery of a competing endogenous RNA regulating autophagy in vascular endothelial cells.
Autophagy
PUBLISHED: 06-01-2014
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MTOR, a central regulator of autophagy, is involved in cancer and cardiovascular and neurological diseases. Modulating the MTOR signaling balance could be of great significance for numerous diseases. No chemical activators of MTOR have been found, and the urgent challenge is to find novel MTOR downstream components. In previous studies, we found a chemical small molecule, 3-benzyl-5-((2-nitrophenoxy) methyl)-dihydrofuran-2(3H)-one (3BDO), that inhibited autophagy in human umbilical vein endothelial cells (HUVECs) and neuronal cells. Here, we found that 3BDO activated MTOR by targeting FKBP1A (FK506-binding protein 1A, 12 kDa). We next used 3BDO to detect novel factors downstream of the MTOR signaling pathway. Activation of MTOR by 3BDO increased the phosphorylation of TIA1 (TIA1 cytotoxic granule-associated RNA binding protein/T-cell-restricted intracellular antigen-1). Finally, we used gene microarray, RNA interference, RNA-ChIP assay, bioinformatics, luciferase reporter assay, and other assays and found that 3BDO greatly decreased the level of a long noncoding RNA (lncRNA) derived from the 3' untranslated region (3'UTR) of TGFB2, known as FLJ11812. TIA1 was responsible for processing FLJ11812. Further experiments results showed that FLJ11812 could bind with MIR4459 targeting ATG13 (autophagy-related 13), and ATG13 protein level was decreased along with 3BDO-decreased FLJ11812 level. Here, we provide a new activator of MTOR, and our findings highlight the role of the lncRNA in autophagy.
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HLA class II alleles may influence susceptibility to adult dermatomyositis and polymyositis in a Han Chinese population.
BMC Dermatol.
PUBLISHED: 05-28-2014
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Polymyositis (PM) and dermatomyositis (DM) are idiopathic inflammatory myopathies. Genetic variability in human leukocyte antigen (HLA) genes plays an important role in the pathogenesis of PM and DM. However, few studies on the subject in Chinese populations have been reported thus far.
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JAK2/STAT3 targeted therapy suppresses tumor invasion via disruption of the EGFRvIII/JAK2/STAT3 axis and associated focal adhesion in EGFRvIII-expressing glioblastoma.
Neuro-oncology
PUBLISHED: 05-25-2014
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As a commonly mutated form of the epidermal growth factor receptor, EGFRvIII strongly promotes glioblastoma (GBM) tumor invasion and progression, but the mechanisms underlying this promotion are not fully understood.
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A new sesquiterpene from the Hainan gorgonian Menella kanisa Grassoff.
Nat. Prod. Res.
PUBLISHED: 05-23-2014
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Chemical investigation of the gorgonian Menella kanisa Grassoff collected from the South China Sea led to the isolation of a new sesquiterpene, menecubebane A (1), together with three known compounds, 8-methoxy-trans-calamenene (2), 8,9-dihydro-linderazulene (3) and (3?)-oleanan-3-ol (4). The structure of 1 was determined by detailed analysis of the spectroscopic data especially the NOESY spectrum. All the compounds were evaluated for their cytotoxic, brine shrimp lethal and antifouling activities.
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[Treatment of thoracolumbar fractures with short-segment transpediclar screw fixation and vertebroplasty via paraspinal intermuscular approach].
Zhongguo Gu Shang
PUBLISHED: 05-16-2014
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To evaluate the clinical effects of short-segment transpedicular fixation and vertebroplasty via paraspinal intermuscular approach in treating thoracolumbar fractures.
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Leaf-templated synthesis of 3D hierarchical porous cobalt oxide nanostructure as direct electrochemical biosensing interface with enhanced electrocatalysis.
Biosens Bioelectron
PUBLISHED: 04-25-2014
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A novel three-dimensional (3D) hierarchical porous cobalt oxide (Co3O4) architecture was first synthesized through a simple, cost-effective and environmentally friendly leaf-templated strategy. The Co3O4 nanoparticles (30-100 nm) with irregular shapes were interconnected with each other to form a 3D multilayer porous network structure, which provided high specific surface area and numerous electrocatalytic active sites. Subsequently, Co3O4 was successfully utilized as direct electrochemical sensing interface for non-enzymatic detection of H2O2 and glucose. By using chronoamperometry, the current response of the sensor at +0.31 V was linear with H2O2 concentration within 0.4-200 ?M with a low limit of detection (LOD) of 0.24 ?M (S/N=3) and a high sensitivity of 389.7 ?A mM(-1) cm(-2). Two linear ranges of 1-300 ?M (with LOD of 0.1 ?M and sensitivity of 471.5 ?A mM(-1) cm(-2)) and 4-12.5 mM were found at +0.59 V for glucose. In addition, the as-prepared sensor showed excellent stability and anti-interference performance for possible interferents such as ascorbic acid, uric acid, dopamine, acetaminophen and especially 0.15 M chloride ions. Similarly, other various metal oxide nanostructures may be also prepared using this similar strategy for possible applications in catalysis, electrochemical sensors, and fuel cells.
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Prevalence, correlates and recognition of depression in Chinese inpatients with cancer.
Gen Hosp Psychiatry
PUBLISHED: 04-18-2014
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To determine the prevalence, correlates and recognition rates of depressive disorders (DDs) in Chinese inpatients with cancer.
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Visible-light-enhanced electrocatalysis and bioelectrocatalysis coupled in a miniature glucose/air biofuel cell.
ChemSusChem
PUBLISHED: 04-18-2014
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A glucose/air biofuel cell (BFC) that can convert both chemical and light energy into electricity is described. Polyterthiophene (pTTh), a photoresponsive conducting polymer, serves as cathode and catalyzes the reduction of oxygen. Taking advantage of the good environmental stability and exceptional optical properties of pTTh, the assembled BFC exhibits excellent stability and a fast photoresponse with an open-circuit voltage (V(oc)) of 0.50?V and a maximum power output density (P(max)) of 23.65??W?cm(-2) upon illumination by visible light of 10?mW?cm(-2) , which is an enhancement of ca. 22?times as compared to P(max) in the dark. Additionally, we propose a possible mechanism for this enhancement. Fabricating a BFC in this manner provides an energy conversion model that offers high efficiency at low cost, paving an avenue for practical solar energy conversion on a large scale.
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Thermal physical property-based fusion of geostationary meteorological satellite visible and infrared channel images.
Sensors (Basel)
PUBLISHED: 03-22-2014
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Geostationary meteorological satellite infrared (IR) channel data contain important spectral information for meteorological research and applications, but their spatial resolution is relatively low. The objective of this study is to obtain higher-resolution IR images. One common method of increasing resolution fuses the IR data with high-resolution visible (VIS) channel data. However, most existing image fusion methods focus only on visual performance, and often fail to take into account the thermal physical properties of the IR images. As a result, spectral distortion occurs frequently. To tackle this problem, we propose a thermal physical properties-based correction method for fusing geostationary meteorological satellite IR and VIS images. In our two-step process, the high-resolution structural features of the VIS image are first extracted and incorporated into the IR image using regular multi-resolution fusion approach, such as the multiwavelet analysis. This step significantly increases the visual details in the IR image, but fake thermal information may be included. Next, the Stefan-Boltzmann Law is applied to correct the distortion, to retain or recover the thermal infrared nature of the fused image. The results of both the qualitative and quantitative evaluation demonstrate that the proposed physical correction method both improves the spatial resolution and preserves the infrared thermal properties.
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The chemokine CXCL9 exacerbates chemotherapy-induced acute intestinal damage through inhibition of mucosal restitution.
J. Cancer Res. Clin. Oncol.
PUBLISHED: 03-11-2014
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Acute intestinal damage induced by chemotherapeutic agent is often a dose-limiting factor in clinical cancer therapy. The aim of this study was to investigate the effect of chemokine CXCL9 on the intestinal damage after chemotherapy and explore the therapeutic potential of anti-CXCL9 agents.
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MicroRNA-566 activates EGFR signaling and its inhibition sensitizes glioblastoma cells to nimotuzumab.
Mol. Cancer
PUBLISHED: 03-11-2014
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Epidermal growth factor receptor (EGFR) is amplified in 40% of human glioblastomas. However, most glioblastoma patients respond poorly to anti-EGFR therapy. MicroRNAs can function as either oncogenes or tumor suppressor genes, and have been shown to play an important role in cancer cell proliferation, invasion and apoptosis. Whether microRNAs can impact the therapeutic effects of EGFR inhibitors in glioblastoma is unknown.
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Identification of suitable reference genes in buffalo grass for accurate transcript normalization under various abiotic stress conditions.
Gene
PUBLISHED: 03-05-2014
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Quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR) is a sensitive technique for normalization of the gene expression level of target genes. Buffalograss (Buchloe dactyloides), a warm-season turfgrass with strong abiotic stress resistance, is widely used in North China. Up to now, no work was performed to evaluate the reference genes in buffalograss. In this study, the expression profiles of ten potential reference genes were examined by qRT-PCR in 24 buffalograss samples, which were subjected to a different treatment (salt, osmotic, cold and heat). Three qRT-PCR analysis methods (GeNorm, NormFinder, and Bestkeeper) were used to evaluate the stability of gene expression. The results indicated that DNAJ and ?-ACTIN were the optimal reference genes for salt-treated leaves, and the combination of PP2A and GAPDH was better reference genes for PEG-treated leaves. Under cold stress, DNAJ and ?-ACTIN showed less variety of expression level in leaves. DNAJ and GAPDH exhibited the most stable expression in heat-treated samples. To sum up, glyceral-dehyde-3-phosphate dehydrogenase (GAPDH), ?-ACTIN, DNAJ-like protein (DNAJ) and protein phosphatase 2A (PP2A) were selected as the most stable reference gene among all tested samples. To further validate the suitability of these reference genes, the expression levels of DREB2 (homologs of AtDREB2) were analyzed in parallel. Our results show that the best reference genes differed across different experimental conditions, and these results should enable better normalization and quantification of transcript levels in buffalograss in the future.
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STAT3 inhibitor WP1066 attenuates miRNA-21 to suppress human oral squamous cell carcinoma growth in vitro and in vivo.
Oncol. Rep.
PUBLISHED: 03-04-2014
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Abnormalities in signal transducer and activator of transcription 3 (STAT3) are involved in the oncogenesis of oral squamous cell carcinoma (OSCC). MicroRNA-21 (miR-21) is an important gene expression regulator to OSCC. miR-21 induction by STAT3 has been reported in multiple human cancers. In the present study, we found that STAT3 (-/p) expression was positively correlated with miR-21 in 60 OSCC samples. A reporter gene assay showed that miR-21 overexpression was dependent on STAT3 activation. WP1066, a small molecular inhibitor of STAT3, was used to suppress STAT3 expression in OSCC cells. TSCCA and TCA8113 showed reduction in tumor cell proliferation, invasion ability and miR-21 expression by WP1066 treatment. In addition, the expression of miR-21 target proteins [programmed cell death 4 (PDCD4), tissue inhibitor of metalloproteinase 3 (TIMP-3) and phosphatase and tensin homolog (PTEN)] was upregulated. Restored STAT3 expression by IL-6 induced miR-21 overexpression, which further confirmed the correlation between STAT3 and miR-21. WP1066 inhibited tumor growth and induced tumor cell apoptosis in the TSCCA xenograft tumor model. Western blotting and immunohistochemistry staining indicated that STAT3 (-/p), Ki67, Bcl-2 and MMP-2 expressions decreased in the WP1066-treated group; PDCD4, TIMP-3 and PTEN expression increased simulta-neously. The present study provides evidence that targeting STAT3 could regulate OSCC cell growth in a miR-21-dependent manner and WP1066 could be a novel candidate drug to treat OSCC by inhibiting STAT3/miR-21 axis.
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BASI, a potent small molecular inhibitor, inhibits glioblastoma progression by targeting microRNA-mediated ?-catenin signaling.
CNS Neurosci Ther
PUBLISHED: 03-03-2014
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The nuclear localization of ?-catenin, a mediator of canonical Wnt signaling, has been indicated in a variety of cancers and is frequently related to tumor progression and metastasis. Therefore, targeting ?-catenin is an attractive therapeutic strategy for cancers.
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Nested PCR-denaturing gradient gel electrophoresis analysis of human skin microbial diversity with age.
Microbiol. Res.
PUBLISHED: 02-23-2014
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To determine whether the composition and structure of skin microbiota differ with age, cutaneous bacteria were isolated from the axillary fossa of 37 healthy human adults in two age groups (old people and young adults). Bacterial genomic DNA was extracted and characterized by nested PCR-denaturing gradient gel electrophoresis (PCR-DGGE) with primers specifically targeting V3 region of the 16S rRNA gene. The excised gel bands were sequenced to identify bacterial categories. The total bacteria, Staphylococcus spp., Staphylococcus epidermidis and Corynebacterium spp. were further enumerated by quantitative PCR. There were no significant differences in the species diversity profiles between age groups. The similarity index was lower across age groups than that it was intra-group. This indicates that the composition of skin flora is more similar to others of the same age than across age groups. While Staphylococcus spp. and Corynebacterium spp. were the dominant bacteria in both groups, sequencing and quantitative PCR revealed that skin bacterial composition differed by age. The copy number of total bacteria and Corynebacterium spp. were significantly lower in younger subjects, whereas there were no statistical differences in the quantity of Staphylococcus spp. and Staphylococcus epidermidis. These results suggest that the skin flora undergo both quantitative and qualitative changes related to aging.
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Development of neural correlates of linear motion in the rat vestibular nucleus.
Sheng Li Xue Bao
PUBLISHED: 02-21-2014
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The capability of the central vestibular system in utilizing cues arising from the inner ear determines the ability of animals to acquire the sense of head orientations in the three-dimensional space and to shape postural movements. During development, neurons in the vestibular nucleus (VN) show significant changes in their electrophysiological properties. An age-dependent enhancement of membrane excitability is accompanied by a progressive increase in firing rate and discharge regularity. The coding of horizontal and vertical linear motions also exhibits developmental refinement in VN neurons. Further, modification of cell surface receptors, such as glutamate receptors, of developing VN neurons are well-orchestrated in the course of maturation, thereby regulating synaptic efficacy and spatial coding capacity of these neurons in local circuits. Taken together, these characteristic features of VN neurons contribute to developmental establishment of space-centered coordinates within the brain.
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A lentivirus-mediated miR-23b sponge diminishes the malignant phenotype of glioma cells in vitro and in vivo.
Oncol. Rep.
PUBLISHED: 02-04-2014
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microRNA (miRNA) sponges are RNA molecules with repeated miRNA binding sequences that can sequester miRNAs from their endogenous target mRNAs, and a stably expressed miRNA sponge is particularly valuable for long-term loss-of-function studies in vitro and in vivo. Glioblastoma multiforme (GBM) is the most common primary brain tumor in adults and is characterized by extraordinarily angiogenic, invasive and migratory capabilities, hallmark features that make the disease incurable. Nonetheless, improvements in clinical treatment and a better understanding of the underlying molecular mechanisms have been achieved within the past few decades. miR-23b has previously been found to function as a tumor oncogene in GBM. In the present study, we employed an microRNA sponge that was forcibly expressed using a lentiviral vector to knock down the expression of miR-23b in vitro and in vivo and assessed the pleiotropic effects on glioma angiogenesis, invasion and migration. We demonstrated that the inhibition of miR-23b in glioma cell lines and orthotopic tumor mouse models resulted in a reduction in tumor malignancy, through the downregulation of HIF-1?, ?-catenin, MMP2, MMP9, VEGF and ZEB1 and increased expression of VHL and E-cadherin. Therefore, we suggest that this miR-23b sponge could be developed into a promising anticancer therapy either alone or in combination with current targeted therapies.
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Ultrasensitive electrochemical sensor for p-nitrophenyl organophosphates based on ordered mesoporous carbons at low potential without deoxygenization.
Anal. Chim. Acta
PUBLISHED: 01-21-2014
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p-Nitrophenyl organophosphates (OPs) including paraoxon, parathion and methyl parathion, etc, are highly poisonous OPs, for which sensitive and rapid detection method is most needed. In this work, an ultrasensitive electrochemical sensor for the determination of p-nitrophenyl OPs was developed based on ordered mesoporous carbons (OMCs) modified glassy carbon electrode (GCE) (OMCs/GCE). The electrochemical behavior and reaction mechanism of p-nitrophenyl OPs at OMCs/GCE was elaborated by taking paraoxon as an example. Experimental conditions such as buffer pH, preconcentration potential and time were optimized. By using differential pulse voltammetry, the current response of the sensor at -0.085 V was linear with concentration within 0.01-1.00 ?M and 1.00-20 ?M paraoxon. Similar linear ranges of 0.015-0.5 ?M and 0.5-10 ?M were found for parathion, and 0.01-0.5 ?M and 0.5-10 ?M for methyl parathion. The low limits of detection were evaluated to be 1.9nM for paraoxon, 3.4 nM for parathion and 2.1 nM for methyl parathion (S/N=3). Common interfering species had no interference to the detection of p-nitrophenyl OPs. The sensor can be applicable to real samples measurement. Therefore, a simple, sensitive, reproducible and cost-effective electrochemical sensor was proposed for the fast direct determination of trace p-nitrophenyl OPs at low potential without deoxygenization.
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Assessment of osteopontin in early breast cancer: correlative study in a randomised clinical trial.
Breast Cancer Res.
PUBLISHED: 01-10-2014
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Osteopontin (OPN) is a malignancy-associated glycoprotein that contributes functionally to tumor aggressiveness. In metastatic breast cancer, we previously demonstrated that elevated OPN in primary tumor and blood was associated with poor prognosis.
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Molecular characterization of skin microbiota between cancer cachexia patients and healthy volunteers.
Microb. Ecol.
PUBLISHED: 01-09-2014
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Systemic inflammation contributes to both the development of cancer and of cachexia. The microenvironment of bacterial habitats might be changed during the progression of cancer cachexia. The aim of this study was to quantitatively and qualitatively compare the composition of the skin microbiota between cancer cachexia patients and healthy volunteers. Cutaneous bacteria were swabbed at the axillary fossa of 70 cancer cachexia patients and 34 healthy individuals from China. Nested-PCR-denaturing gradient gel electrophoresis (PCR-DGGE) with primers specifically targeting V3 region and quantitative PCR (qPCR) for total bacteria, Corynebacterium spp., Staphylococcus spp., and Staphylococcus epidermidis were performed on all samples. Barcoded 454 pyrosequencing of the V3-V4 regions was performed on 30 randomly selected samples. By comparing diversity and richness indices, we found that the skin microbiome of cachectic cancer patients is less diverse than that of healthy participants, though these differences were not significant. The main microbes that reside on human skin were divided into four phyla: Firmicutes, Actinobacteria, Proteobacteria, and Bacteroidetes. Staphylococcus spp. and Corynebacterium spp. were the dominant bacteria at the genus level. Significantly fewer Corynebacterium spp. had been observed in cachexia patients compared to healthy subjects. These results suggest that the presence of cancer and cachexia alters human skin bacterial communities. Understanding the changes in microbiota during cancer cachexia may lead to new insights into the syndrome.
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ZnS nanoarchitectures induced dysfunction of vascular endothelial cells in vitro and in vivo.
Environ. Toxicol.
PUBLISHED: 01-07-2014
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ZnS nanoarchitectures have been intensively investigated recently because of their applications in optoelectronics and adsorption capacity. The potential hazard of ZnS nanoarchitectures is not well known. In this study, we investigated the toxicity of ZnS nanoarchitectures on vascular endothelial cell (VEC) in vitro and in vivo. The results showed that ZnS could inhibit human umbilical vein endothelial cell (HUVEC) proliferation at 50 and 200 ?g/mL. Endothelial nitric oxide synthase (eNOS) activity, nitric oxide (NO), and reactive oxygen species productions were increased, which was companied with the decrease in caveolin-1 level. The endothelium of the aortic root was damaged and the NO levels in serum were elevated in the mice treated with 5 or 10 mg/kg ZnS for 3 and 6 days, but the body could repair the damage. The data suggested that the high concentration of ZnS could induce dysfunction of VECs through decreasing caveolin-1 and elevation of the eNOS activity and thus present toxicity. © 2014 Wiley Periodicals, Inc. Environ Toxicol, 2014.
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AJAP1 is dysregulated at an early stage of gliomagenesis and suppresses invasion through cytoskeleton reorganization.
CNS Neurosci Ther
PUBLISHED: 01-06-2014
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Down-regulation of AJAP1 in glioblastoma multiforme (GBM) has been reported. However, the expression profiles of AJAP1 in gliomas and the underlying mechanisms of AJAP1 function on invasion are still poorly understood.
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Enhancement of microbial hydroxylation of 13-ethyl-gon-4-ene-3,17-dione by Metarhizium anisopliae using nano-liposome technique.
J. Ind. Microbiol. Biotechnol.
PUBLISHED: 01-02-2014
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The introduction of 11?-hydroxy to 13-ethyl-gon-4-ene-3,17-dione (GD) by microbial transformation is a key step in the synthesis of oral contraceptive desogestrel, while low substrate solubility and uptake into cells are tough problems influencing biotransformation efficiency greatly. Nano-liposome technique was used in the hydroxylation of GD by Metarhizium anisopliae. The substrate GD was processed to be GD-loaded nano-liposomes (GNLs) with high stability and encapsulation efficiency, and then applied in microbial hydroxylation by M. anisopliae. The results proved that the yield of the main product 11?-hydroxy-13-ethyl-gon-4-ene-3,17-dione (HGD) tripled compared to regular solvent dimethylformamide dispersion method at 2 g/l of substrate feeding concentration, and the HGD conversion rate showed no obvious reduction when the substrate feeding concentration increased from 2 to 6 g/l, which indicated the improvement of GNL addition method on biotransformation. Furthermore, the main byproduct changed from 6?-hydroxy derivative of GD (with similar polarity to HGD) to 6?,11?-dihydroxy derivative, which benefits the following purification of HGD from fermentation broth. These advantages suggest a great potential for the application of nano-liposome technique in microbial steroid transformation.
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miRNA-124 down-regulates SOX8 expression and suppresses cell proliferation in non-small cell lung cancer.
Int J Clin Exp Pathol
PUBLISHED: 01-01-2014
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Non-small lung cell carcinoma (NSCLC) is a leading lethal disease and a global health burden. The function of the Sex determining region Y (SRY)-related high mobility group box (SOX) family gene in cancer has attracted the attention of more and more scientists recently, yet there are few reports regarding the role of SOX in NSCLC. Our study aimed to investigate the expression of SOX8, a protein belonging to the E group of the SOX family, as well as SOX9, in non-small cell lung cancer (NSCLC) and the relationship of gene expression to clinicopathological factors and prognosis in patients. Immunohistochemical analysis was used to measure the expression of SOX8 in 80 NSCLC and 7 adjacent normal tissues. SOX8 expression was detected as elevated in tumor samples and correlated to tumor size (P < 0.001), lymph node metastasis (P = 0.001), differentiation classification (P = 0.015), and clinical stage (P = 0.013) significantly. Moreover, Kaplan-Meier survival analysis demonstrated that shorter survival time for patients who had higher SOX8 expression (P < 0.001). In addition, our experiments indicate that miRNA-124 functions as a tumor suppressor in NSCLC. We also demonstrate miRNA-124 directly targeted and decreased SOX8 in NSCLC cell lines, suggesting smiRNA-124 may regulate NSCLC cell proliferation via decreasing SOX8 (oncogenicity of biomarker in NSCLC).
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Operating Mechanism and Molecular Dynamics of Pheromone-Binding Protein ASP1 as Influenced by pH.
PLoS ONE
PUBLISHED: 01-01-2014
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Odorant binding protein (OBP) is a vital component of the olfactory sensation system. It performs the specific role of ferrying odorant molecules to odorant receptors. OBP helps insects and types of animal to sense and transport stimuli molecules. However, the molecular details about how OBPs bind or release its odorant ligands are unclear. For some OBPs, the systems' pH level is reported to impact on the ligands' binding or unbinding capability. In this work we investigated the operating mechanism and molecular dynamics in bee antennal pheromone-binding protein ASP1 under varying pH conditions. We found that conformational flexibility is the key factor for regulating the interaction of ASP1 and its ligands, and the odorant binds to ASP1 at low pH conditions. Dynamics, once triggered by pH changes, play the key roles in coupling the global conformational changes with the odorant release. In ASP1, the C-terminus, the N-terminus, helix ?2 and the region ranging from helices ?4 to ?5 form a cavity with a novel 'entrance' of binding. These are the major regions that respond to pH change and regulate the ligand release. Clearly there are processes of dynamics and hydrogen bond network propagation in ASP1 in response to pH stimuli. These findings lead to an understanding of the mechanism and dynamics of odorant-OBP interaction in OBP, and will benefit chemsensory-related biotech and agriculture research and development.
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The effect of two novel amino acid-coated magnetic nanoparticles on survival in vascular endothelial cells, bone marrow stromal cells, and macrophages.
Nanoscale Res Lett
PUBLISHED: 01-01-2014
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Magnetic nanoparticles (MNPs) have been popularly used in many fields. Recently, many kinds of MNPs are modified as new absorbents, which have attracted considerable attention and are promising to be applied in waste water. In our previous study, we synthesized two novel MNPs surface-coated with glycine or lysine, which could efficiently remove many anionic and cationic dyes under severe conditions. It should be considered that MNP residues in water may exert some side effects on human health. In the present study, we evaluated the potential nanotoxicity of MNPs in human endothelial cells, macrophages, and rat bone marrow stromal cells. The results showed that the two kinds of nanoparticles were consistently absorbed into the cell cytoplasm. The concentration of MNPs@Gly that could distinctly decrease survival was 15 ?g/ml in human umbilical vascular endothelial cells (HUVECs) or bone marrow stromal cells (BMSCs) and 10 ?g/ml in macrophages. While the concentration of MNPs@Lys that obviously reduced viability was 15 ?g/ml in HUVECs or macrophages and 50 ?g/ml in BMSCs. Furthermore, cell nucleus staining and cell integrity assay indicated that the nanoparticles induced cell apoptosis, but not necrosis even at a high concentration. Altogether, these data suggest that the amino acid-coated magnetic nanoparticles exert relatively high cytotoxicity. By contrast, lysine-coated magnetic nanoparticles are more secure than glycine-coated magnetic nanoparticles.
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Antepartum or immediate postpartum renal biopsies in preeclampsia/eclampsia of pregnancy: new morphologic and clinical findings.
Int J Clin Exp Pathol
PUBLISHED: 01-01-2014
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Preeclampsia (PE) and eclampsia remain leading causes of maternal and fetal mortality worldwide. The kidney is considered the first and most severely affected organ in women with PE/eclampsia. In this study, we analyzed new morphologic features of kidney biopsies and clinical findings in patients with PE or eclampsia at our hospital.
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Identification of a core miRNA-pathway regulatory network in glioma by therapeutically targeting miR-181d, miR-21, miR-23b, ?-Catenin, CBP, and STAT3.
PLoS ONE
PUBLISHED: 01-01-2014
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The application of microRNAs (miRNAs) in the therapeutics of glioma and other human diseases is an area of intense interest. However, it's still a great challenge to interpret the functional consequences of using miRNAs in glioma therapy. Here, we examined paired deep sequencing expression profiles of miRNAs and mRNAs from human glioma cell lines after manipulating the levels of miRNAs miR-181d, -21, and -23b, as well as transcriptional regulators ?-catenin, CBP, and STAT3. An integrated approach was used to identify functional miRNA-pathway regulatory networks (MPRNs) responding to each manipulation. MiRNAs were identified to regulate glioma related biological pathways collaboratively after manipulating the level of either post-transcriptional or transcriptional regulators, and functional synergy and crosstalk was observed between different MPRNs. MPRNs responsive to multiple interventions were found to occupy central positions in the comprehensive MPRN (cMPRN) generated by integrating all the six MPRNs. Finally, we identified a core module comprising 14 miRNAs and five pathways that could predict the survival of glioma patients and represent potential targets for glioma therapy. Our results provided novel insight into miRNA regulatory mechanisms implicated in therapeutic interventions and could offer more inspiration to miRNA-based glioma therapy.
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Identification of miRNA-mediated core gene module for glioma patient prediction by integrating high-throughput miRNA, mRNA expression and pathway structure.
PLoS ONE
PUBLISHED: 01-01-2014
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The prognosis of glioma patients is usually poor, especially in patients with glioblastoma (World Health Organization (WHO) grade IV). The regulatory functions of microRNA (miRNA) on genes have important implications in glioma cell survival. However, there are not many studies that have investigated glioma survival by integrating miRNAs and genes while also considering pathway structure. In this study, we performed sample-matched miRNA and mRNA expression profilings to systematically analyze glioma patient survival. During this analytical process, we developed pathway-based random walk to identify a glioma core miRNA-gene module, simultaneously considering pathway structure information and multi-level involvement of miRNAs and genes. The core miRNA-gene module we identified was comprised of four apparent sub-modules; all four sub-modules displayed a significant correlation with patient survival in the testing set (P-values?0.001). Notably, one sub-module that consisted of 6 miRNAs and 26 genes also correlated with survival time in the high-grade subgroup (WHO grade III and IV), P-value?=?0.0062. Furthermore, the 26-gene expression signature from this sub-module had robust predictive power in four independent, publicly available glioma datasets. Our findings suggested that the expression signatures, which were identified by integration of miRNA and gene level, were closely associated with overall survival among the glioma patients with various grades.
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Ion uptake in tall fescue as affected by carbonate, chloride, and sulfate salinity.
PLoS ONE
PUBLISHED: 01-01-2014
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Turfgrass nutrient uptake may be differentially affected by different salts. The objective of this study was to compare nutrient uptake in tall fescue (Festuca arundinacea Schreb.) as affected by carbonate, chloride, and sulfate under iso-osmotic, iso-Na+ strength conditions. 'Tar Heel II' and 'Wolfpack' cultivars were subjected to NaCl, Na2CO3, Na2SO4, CaCl2, NaCl+ CaCl2, Na2CO3+ CaCl2, and Na2SO4+ CaCl2, in the range of 0 to 225 mM. There was no cultivar difference regarding K, Na, Mg, and Mn content in shoots. 'Tar Heel II' had higher shoot Ca content than 'Wolfpack', which were 6.9 and 5.7 g kg(-1), respectively. In general, K+/Na+ ratio decreased with increasing salt concentrations, which reached <1 at about 87.5 mM in Na2CO3 treatment. All salt treatments decreased Mg content in shoot tissues, especially in Na2CO3 and treatments containing CaCl2. Both Ca and Mg content in shoot were higher in the NaCl treatment than the Na2SO4 and Na2CO3 treatments. All salt treatments except Na2CO3 had higher Mn content in shoots compared to the control. In conclusion, nutrient uptake was differently affected by carbonate, chloride, and sulfate which are different in pH, electrical conductivity (EC), and osmotic potential at the same concentration. Adding Ca to the sodium salts increased Ca content and balanced K+/Na+ in shoots, but did not increase Mg content, which was below sufficient level. Maintaining Mg content in shoots under salinity stress was recommended. The physiological impact of elevated Mn content in shoot under salinity stress requires further study.
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Experience of offering HIV rapid testing to at-risk patients in community health centers in eight Chinese cities.
PLoS ONE
PUBLISHED: 01-01-2014
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To explore the feasibility of offering HIV counseling and testing in community health centers (CHCs) and to provide evidence for the HIV/AIDS response in China.
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A Facile One-Pot Method to Synthesize a Polypyrrole/Hemin Nanocomposite and Its Application in Biosensor, Dye Removal, and Photothermal Therapy.
ACS Appl Mater Interfaces
PUBLISHED: 12-11-2013
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In this work, we introduced a facile method for the construction of a polypyrrole/hemin (PPy/hemin) nanocomposite via one-pot chemical oxidative polymerization. In this process, a hemin molecule serving as a dopant was entrapped in the PPy nanocomposite during chemical oxidative polymerization. Scanning electron microscopy (SEM), transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FTIR), and UV-visible spectroscopy results demonstrated that the PPy/hemin nanocomposite was successfully synthesized. The as-prepared nanocomposite exhibited intrinsic peroxidase-like catalytic activities, strong adsorption properties, and an excellent near-infrared (NIR) light-induced thermal effect. We utilized the nanomaterials to catalyze the oxidation of a peroxidase substrate 3,3,5,5-tetramethylbenzidine by H2O2 to the oxidized colored product which provided a colorimetric detection of glucose. As low as 50 ?M glucose could be detected with a linear range from 0.05 to 8 mM. Moreover, the obtained nanocomposite also showed excellent removal efficiency for methyl orange and rhodamine B and a photothermal effect, which implied a promising application as the pollutant adsorbent and photothermal agent. The unique nature of the PPy/hemin nanocomposite makes it very promising for the fabrication of inexpensive, high-performance bioelectronic devices in the future.
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Facile Fabrication of Highly Efficient g-C3N4/Ag2O Heterostructured Photocatalysts with Enhanced Visible-Light Photocatalytic Activity.
ACS Appl Mater Interfaces
PUBLISHED: 11-19-2013
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Highly efficient visible-light-driven g-C3N4/Ag2O heterostructured photocatalysts were prepared by a simple liquid phase synthesis method at room temperature. The composition, structure, morphology, and optical absorption properties of the as-prepared g-C3N4/Ag2O composites were characterized by XRD, FTIR, XPS, TEM, and UV-vis DRS, respectively. We found interestingly that the photogenerated charge carriers separations of the as-prepared g-C3N4/Ag2O composites were closely related to the mass ratio of g-C3N4 and Ag2O. When the mass ratio of g-C3N4 and Ag2O reached 1:4, the as-prepared composite exhibited the highest photocatalytic activity, which was almost 11 and 1.2 times as high as that of individual g-C3N4 and Ag2O, respectively. The enhancement of photocatalytic activity could be attributed to the synergetic effects between g-C3N4 and Ag2O as well as the improved dispersibility and the decreased particle size of Ag2O. Moreover, the as-prepared composites showed excellent stability toward the photodegradation of methyl orange (MO). Finally, a possible photocatalytic and charge separation mechanism was proposed.
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HOTAIR, a cell cycle-associated long noncoding RNA and a strong predictor of survival, is preferentially expressed in classical and mesenchymal glioma.
Neuro-oncology
PUBLISHED: 11-07-2013
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Long noncoding RNA Hox transcript antisense intergenic RNA (HOTAIR) has been characterized as a negative prognostic factor in breast and colon cancer patients. The clinical significance and function of HOTAIR in glioma remains unclear.
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High-performance BiOBr ultraviolet photodetector fabricated by a green and facile interfacial self-assembly strategy.
Nanoscale
PUBLISHED: 11-07-2013
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We have fabricated a BiOBr film for the first time through water-air interfacial self-assembly, which is a green, easily processable and environmentally friendly strategy. Moreover, an ultraviolet photodetector based on the BiOBr film exhibited excellent stability and a fast response time.
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A light transmission technique for pore size measurement in track-etched membranes.
Chem. Commun. (Camb.)
PUBLISHED: 10-31-2013
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A new approach is proposed for accurate measurement of the pore sizes in a track-etched PET membrane (polyethylene terephthalate) from a nanometer scale to a submicrometer scale, which only entailed UV-Vis spectrometry.
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Recombinant lipoproteins reinforce cytotoxicity of doxorubicin to hepatocellular carcinoma.
J Drug Target
PUBLISHED: 10-04-2013
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Abstract Cancer nanotherapeutics are changing the landscape of tumor treatment and used to circumvent limitations of conventional chemotherapy, such as non-specificity and low bioavailability. Reconstituted high density lipoproteins (rHDL) system is one of the most promising targeting delivery systems of chemotherapeutic drugs toward tumors. Here, we developed recombined high-density lipoprotein which can be functionalized to deliver doxorubicin intracellular with a higher efficiency. The cellular viability assay showed that the rHDL/Dox nanovectors had an enhanced efficiency in inhibiting the cell viability of hepatocellular carcinoma cell lines HepG2 and SMMC-7721. FACS and confocal microscopy was used to observe the doxorubicin delivery into cancer cells. Intracellular drug accumulation analysis confirmed that treatment of rHDL/Dox nanovectors resulted in higher intracellular doxorubicin concentration to the levels exceeding that of free drug. On the premise of efficient drug delivery, rHDL/Dox nanovectors have been preliminarily demonstrated effective inducing of cytotoxic effect and cell apoptosis to both of the cell lines in vitro. Tissue distribution experiment showed that rHDL/Dox nanovectors could also deliver doxorubicin to liver effectively. So, we proposed that this lipoprotein-based strategy holds promise for a safer and more efficient delivery of chemotherapeutic agents in the treatment of hepatocellular carcinoma.
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[The prevalence and influencing factors of eye diseases for IT industry video operation workers].
Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi
PUBLISHED: 10-02-2013
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To investigate the situation of video-contact and eye diseases for IT industry video operation workers, and to analyze the influencing factors, providing scientific evidence for the make of health-strategy for IT industry video operation workers.
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TiO(2) nanotube arrays: intrinsic peroxidase mimetics.
Chem. Commun. (Camb.)
PUBLISHED: 10-01-2013
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TiO2 nanotube arrays (NTA), prepared by potentiostatic anodization, were discovered to possess an intrinsic peroxidase-like activity. The colorimetric and electrochemical assays both demonstrated their excellent catalytic activity towards H2O2 reduction. On this basis, a simple and inexpensive electrochemical biosensor for glucose detection was developed.
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[Analysis of risk factors for dry eye syndrome in visual display terminal workers].
Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi
PUBLISHED: 09-24-2013
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To analyze the risk factors for dry eye syndrome in visual display terminal (VDT) workers and to provide a scientific basis for protecting the eye health of VDT workers.
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[The impact of community-based organizations in HIV testing mobilization among men who have sex with men].
Zhonghua Yu Fang Yi Xue Za Zhi
PUBLISHED: 08-21-2013
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To analyze the impact of efforts of community-based organizations (CBO) in HIV testing mobilization and case finding among men who have sex with men(MSM).
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Septicemia caused by Leifsonia aquatica in a healthy patient after retinal reattachment surgery.
J. Clin. Microbiol.
PUBLISHED: 08-14-2013
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Leifsonia aquatica is an aquatic bacterium that is typically found in environmental water habitats. Infections due to L. aquatica are rare and commonly catheter associated in immunocompromised patients. We report the first case of an acute septicemia caused by L. aquatica in a healthy immunocompetent host after cryopexy in the absence of a catheter.
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Activated hepatic stellate cells promote hepatocellular carcinoma cell migration and invasion via the activation of FAK-MMP9 signaling.
Oncol. Rep.
PUBLISHED: 07-23-2013
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Activated hepatic stellate cells (HSCs) are the major subtype of stromal cells in the liver tumor microenvironment which can promote the growth and migration of hepatocellular carcinoma (HCC) cells. However, the underlying mechanisms by which activated HSCs exert their oncogenic effects are not fully understood to date. In the present study, we investigated the number of activated HSCs and its clinicopathological significance in HCC and uncovered its correlation with focal adhesion kinase (FAK)-MMP9 signaling. A higher number of activated HSCs was associated with tumor invasion of the portal vein, advanced TNM stage and poorer tumor differentiation. The number of activated HSCs was positively correlated with the expression levels of p-FAK and MMP9 in HCC. Furthermore, we studied the effects of activated HSCs on the migration and invasion of HCC cells in vitro. Conditioned medium (CM) from activated HSCs or co-culture with activated HSCs significantly induced the migration and invasion of HCC cells. In addition, activation of FAK-MMP9 signaling in HCC was demonstrated in the presence of activated HSC-CM and of co-culture. Inhibition of FAK-MMP9 signaling in HCC cells with FAK short hairpin RNA (shRNA) abrogated the effects of activated HSCs on HCC cells. Taken together, our data suggest that activated HSCs in the tumor microenvironment promote HCC cell migration and invasion via activation of FAK-MMP9 signaling.
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Protection of vascular endothelial cells injured by angiotensin II and hypoxia in vitro by Ginkgo biloba (Ginaton).
Vasc Endovascular Surg
PUBLISHED: 07-23-2013
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The objective of this study was to explore the protective effect and the possible mechanism of Ginkgo biloba extract (Ginaton) on human vascular endothelial cells (VECs) injured by angiotensin II (Ang-II) and hypoxia. The human aortic VECs were divided into different groups to observe the changes in endothelin (ET), calcium concentration ([Ca(2+)]i), and mitochondrial membrane potential (MMP). The results showed that Ginaton had inhibited ET secretion induced by hypoxia and Ang-II (P < .01). the protection offered by Ginaton at mid (10 mg/mL) and low (5 mg/mL) concentrations was obviously better than that offered at high concentration (25 mg/mL). The [Ca(2+)]i increased and MMP decreased significantly in both hypoxia group and Ang-II group (P < .01); however, the changes in [Ca(2+)]i and MMP could be meliorated by Ginaton. This study suggested that Ginaton could effectively protect VECs against injury, and the dose used clinically would rather be low than too high for getting better results.
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Visual and semi-quantitative analyses of dual-phase breast-specific gamma imaging with Tc-99m-sestamibi in detecting primary breast cancer.
Ann Nucl Med
PUBLISHED: 07-17-2013
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Breast cancer is the most common malignancy for females worldwide. This study was to evaluate the application of dual-phase breast-specific gamma imaging (BSGI) in detecting primary breast cancer.
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Blockage of a miR-21/EGFR regulatory feedback loop augments anti-EGFR therapy in glioblastomas.
Cancer Lett.
PUBLISHED: 07-17-2013
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Epidermal growth factor receptors (EGFR) expression is frequently amplified in human glioblastoma cells. Nimotuzumab, a monoclonal antibody (mAb) against EGFR, has been used globally in clinics as an anti-cancer agent. It is largely unknown whether the blockade of miR-21, a microRNA that is upregulated in glioma cells, could amplify the effects of nimotuzumab. Herein, we have demonstrated that miR-21 directly targets von Hippel-Lindau (VHL) and peroxisome-proliferator-activated receptor ? (PPAR?) and that miR-21 regulates EGFR/AKT signaling through VHL/?-catenin and the PPAR?/AP-1 axis. Further, the expression of miR-21 is regulated by EGFR via the activation of ?-catenin and AP-1. These data indicate that a feedback loop exists between miR-21 and EGFR. We also show that the combination of nimotuzumab and an inhibitor of miR-21 is superior to single-agent therapy. These results clarify a novel association between miR-21 and EGFR in the regulation of cancer cell progression.
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Association between LMP2/LMP7 genetic variability and the metastasis risk of ovarian cancer in Chinese women in Beijing.
Hum. Immunol.
PUBLISHED: 07-11-2013
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LMP2/LMP7 gene (LMP, low molecular mass protein) perform a critical role in the foreign antigen processing via the major histocompatibility complex-I (MHC-I) complex CD8(+) cytotoxic T lymphocytes (CTL) pathway. This study was designed to investigate whether the sequence variants in LMP2/LMP7 gene would increase the risk of ovarian cancer in the Chinese population. Total of 235 patients with ovarian cancer and 338 normal controls were recruited. Two polymorphisms of LMP2-60 (Arg?His) and LMP7-145 (Gln?Lys) were identified by PCR-RFLP (RFLP, restriction fragment length polymorphism) method. The association of LMP2/LMP7 gene variations with ovarian cancer was assessed by logistic regression analysis. The results revealed that LMP7-145 Gln/Lys and Lys/Lys alleles were associated with the risk of ovarian cancer (P=0.002, OR=2.47; P<0.001, OR=3.23). Meanwhile, the relationship between the LMP7-145 polymorphism and the lymph node metastasis and tumor distant metastasis were also found. No statistical correlation between any of the LMP2-60 polymorphic genotypes and the ovarian cancer clinicopathological characteristics were observed (P>0.05). These results suggested that LMP7 genetic variant could increase the susceptibility to ovarian cancer development; especially increase the risk of lymph node and tumor distant metastasis.
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Regulating effect of Tea polyphenols on Endothelin, Intracellular Calcium Concentration and Mitochondrial Membrane Potential in Vascular Endothelial Cells Injured by Angiotensin II.
Ann Vasc Surg
PUBLISHED: 07-04-2013
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Tea polyphenols is a mainly active component of tea, and it is considered as a reagent for anti-atherosclerosis, protecting injuries of vascular endothelial cells and preventing cardiovascular diseases. The content of endothelin, intracellular calcium concentration and mitochondrial membrane potential in vascular endothelial cells directly reflects the function and injured status of cells. The objective of this study was to study the regulating effects of Tea polyphenols on endothelin, intracellular calcium concentration and mitochondrial membrane potential in vascular endothelial cells injured by Angiotensin II then explore the protective effect of Tea polyphenols on human vascular endothelial cells.
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AC1MMYR2, an inhibitor of dicer-mediated biogenesis of Oncomir miR-21, reverses epithelial-mesenchymal transition and suppresses tumor growth and progression.
Cancer Res.
PUBLISHED: 06-28-2013
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The extensive involvement of miRNAs in cancer pathobiology has opened avenues for drug development based on oncomir inhibition. Dicer is the core enzyme in miRNA processing that cleaves the terminal loop of precursor microRNAs (pre-miRNAs) to generate mature miRNA duplexes. Using the three-dimensional structure of the Dicer binding site on the pre-miR-21 oncomir, we conducted an in silico high-throughput screen for small molecules that block miR-21 maturation. By this method, we identified a specific small-molecule inhibitor of miR-21, termed AC1MMYR2, which blocked the ability of Dicer to process pre-miR-21 to mature miR-21. AC1MMYR2 upregulated expression of PTEN, PDCD4, and RECK and reversed epithelial-mesenchymal transition via the induction of E-cadherin expression and the downregulation of mesenchymal markers, thereby suppressing proliferation, survival, and invasion in glioblastoma, breast cancer, and gastric cancer cells. As a single agent in vivo, AC1MMYR2 repressed tumor growth, invasiveness, and metastasis, increasing overall host survival with no observable tissue cytotoxicity in orthotopic models. Our results offer a novel, high-throughput method to screen for small-molecule inhibitors of miRNA maturation, presenting AC1MMYR2 as a broadly useful candidate antitumor drug.
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Modified Jiu Wei Qiang Huo decoction improves dysfunctional metabolomics in influenza A pneumonia-infected mice.
Biomed. Chromatogr.
PUBLISHED: 06-26-2013
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In order to study the effective mechanism of a traditional Chinese medicine (TCM), modified Jiu Wei Qiang Huo decoction (MJWQH), against H1N1-induced pneumonia in mice, we chose a holistic approach. A reverse-phase liquid chromatography with quadruple time-of-flight mass spectrometry (LC-Q-TOF-MS) was developed to determine metabolomic biomarkers in mouse serum for the MJWQH effects. Thirteen biomarkers of H1N1-induced pneumonia in mice serum were identified, which comprised L-valine, lauroylcarnitine, palmitoyl-L-carnitine, L-ornithine, uric acid, taurine, O-succinyl-L-homoserine, L-leucine, L-phenylalanine, PGF2?, 20-ethyl-PGE2, arachidonic acid, and glycerophospho-N-arachidonoyl ethanolamine. Among them, metabolites of amino acids, fatty acids and arachidonic acid had the most relevant changes in mice with H1N1-induced pneumonia. MJWQH effectively improved weight loss, lung index, biomarkers and inflammatory mediators such as prostaglandin E2 and phospholipase A2 in the infected mice. Importantly, MJWQH reversed the elevated biomarkers to the control levels from the infection, which provided a systematic view and a theoretical basis for its prevention or treatment. The results suggest that the protective effect of MJWQH against H1N1-induced pneumonia is possibly through regulation of pathways for amino acid, fatty acid and arachidonic acid metabolism. They also suggest that the LC-MS-based metabolomic strategy is a powerful tool for elucidation of the mechanisms of TCM. Copyright © 2013 John Wiley & Sons, Ltd.
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Protective effect of Aplysin on hepatic injury in ethanol-treated rats.
Food Chem. Toxicol.
PUBLISHED: 06-09-2013
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This study evaluated the protective effects of Aplysin against ethanol-induced hepatic injury in rats and analyzed the associated mechanisms. Rats were administered orally with ethanol 8-12ml/kg bw excluding the rats in the control group at 1h after rats were administered by gavage doses of Aplysin (50, 100, and 150mg/kg bw) every day. After 6weeks, rats were sacrificed, and liver injury was evaluated by biochemical and pathological examination. Hepatocyte apoptosis was analyzed by annexin V-FITC/PI staining. Ethanol metabolic enzymes, oxidative stress, mitochondrial function, and Bcl-2, Bax, cytochrome c and cleaved caspase-3 expressions were evaluated by western blot analysis. These results demonstrated that Aplysin exhibited a significant hepatoprotective effect. In the ethanol-treated group, cytochrome P4502E1 and alcohol dehydrogenase were increased significantly in liver tissue. Moreover, Aplysin not only significantly reversed the ratio of NAD(+)/NADH and mitochondrial glutathione depletion, but also reversed the decreased activity of mitochondrial respiratory chain complexes I, III and IV. Overexpression of cytoplasmic cytochrome c and caspase-3 activation was suppressed by Aplysin. These results suggest that Aplysin alleviates hepatocyte apoptosis by modulating the ethanol-metabolizing pathway, attenuating oxidative stress, ameliorating mitochondrial function, inhibiting mitochondrial damage-mediated apoptosis, which ultimately prevent and repair alcoholic liver injury.
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miR-19a and miR-19b overexpression in gliomas.
Pathol. Oncol. Res.
PUBLISHED: 05-28-2013
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Astrocytic gliomas are the most common type of human primary brain tumors with poor prognosis. MicroRNAs(miRs) are frequently deregulated in gliomas and play an oncogenic or tumor suppressor role. In our previous study we found that miR-19a and miR-19b were up-regulated in malignant glioma cell lines by microRNA array. For further validation of this finding, the expression of miR-19a and miR-19b was detected by qRT-PCR and in situ hybridization(ISH) in 8 malignant glioma cell lines, 43 freshly resected glioma samples and 75 archival paraffin embedded glioma specimens with different grades of malignancy in the present study. The results demonstrate that miR-19a and miR-19b are overexpressed in glioma cell lines and astrocytic glioma tissues, and their expression level is positively correlated with tumor grades. Additionally, the tumor suppressor gene PTEN is identified as the target of miR-19a and miR-19b by Luciferase assay. It is speculated that miR-19a and miR-19b may have an oncogenic role in gliomagenesis at least partially via the negative regulation of PTEN and the molecular mechanism of gliomagenesis in which miR 19a and miR-19b involved should be investigated further.
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Protective effects of ginaton on vascular endothelial cells injured by angiotensin II and hypoxia in vitro.
Vasc Endovascular Surg
PUBLISHED: 05-20-2013
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The objective of this study was to explore the protective effect and possible mechanism of Ginkgo biloba extract (Ginaton) on human vascular endothelial cells (VECs) injured by angiotensin II (Ang-II) and hypoxia. The human aortic VECs were divided into different groups to observe the changes in endothelin (ET), intracellular calcium concentration ([Ca(2+)]i), and mitochondrial membrane potential (MMP). The results showed that Ginaton had inhibited ET secretion induced by hypoxia and Ang-II (P < .01); the protective effects of mid (10 mg/mL) and low concentrations (5 mg/mL) of Ginaton was obviously higher than that of the high concentration (25 mg/mL); [Ca(2+)]i increased and MMP decreased significantly in both the hypoxia and the Ang-II groups (P < .01); however, the changes in [Ca(2+)]i and MMP could be meliorated by Ginaton. This study suggested that Ginaton could effectively protect VECs against injury, and lower dose would be used clinically rather than the higher dose for obtaining better results.
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[Anatomical study on the treatment of complex acetabular fractures with self-designed 3-dimensional anatoimical locking plate].
Zhongguo Gu Shang
PUBLISHED: 05-18-2013
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To design a new 3-dimensional anatomical locking plate internal fixation on the basis of anatomic character of acetabulum for treating complex acetabular fractures except the posterior wall and posterior column fracture, and to investigate its advantages and disadvantages.
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Facile synthesis of a free-standing Ag@AgCl film for a high performance photocatalyst and photodetector.
Chem. Commun. (Camb.)
PUBLISHED: 04-24-2013
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A novel and facile method is developed to prepare an Ag@AgCl film which exhibits high photocatalytic activity under visible light irradiation. In addition, it can also be used to fabricate a photodetector that possesses a fast response time and good photocurrent reproducibility.
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The Relationship between DNA Sequences and Oligonucleotide-Templated Silver Nanoclusters and Their Fluorescence Properties.
Chemistry
PUBLISHED: 04-18-2013
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A novel approach was developed to study the relationship between DNA sequences and DNA-templated silver nanoclusters (DNA-Ag NCs) in detail by using an ordinary DNA strand as an example. Three kinds of Ag NCs are formed by using the DNA strand as a scaffold. By dividing the DNA template into several parts according to their different affinities to Ag(+) , it was found that the fluorescence properties of DNA-Ag NCs are related to not only the sequences but also to the position of different parts in the template, which provides a more efficient approach to obtain DNA-Ag NCs with required photoluminescence properties and may ultimately contribute to the targeted synthesis of DNA-Ag NCs.
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Immune adaptor ADAP in T cells regulates HIV-1 transcription and cell-cell viral spread via different co-receptors.
Retrovirology
PUBLISHED: 04-09-2013
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Immune cell adaptor protein ADAP (adhesion and degranulation-promoting adaptor protein) mediates aspects of T-cell adhesion and proliferation. Despite this, a connection between ADAP and infection by the HIV-1 (human immunodeficiency virus-1) has not been explored.
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What is Visualize?

JoVE Visualize is a tool created to match the last 5 years of PubMed publications to methods in JoVE's video library.

How does it work?

We use abstracts found on PubMed and match them to JoVE videos to create a list of 10 to 30 related methods videos.

Video X seems to be unrelated to Abstract Y...

In developing our video relationships, we compare around 5 million PubMed articles to our library of over 4,500 methods videos. In some cases the language used in the PubMed abstracts makes matching that content to a JoVE video difficult. In other cases, there happens not to be any content in our video library that is relevant to the topic of a given abstract. In these cases, our algorithms are trying their best to display videos with relevant content, which can sometimes result in matched videos with only a slight relation.