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Find video protocols related to scientific articles indexed in Pubmed.
A Multi-label Classifier for Prediction Membrane Protein Functional Types in Animal.
J. Membr. Biol.
PUBLISHED: 08-09-2014
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Membrane protein is an important composition of cell membrane. Given a membrane protein sequence, how can we identify its type(s) is very important because the type keeps a close correlation with its functions. According to previous studies, membrane protein can be divided into the following eight types: single-pass type I, single-pass type II, single-pass type III, single-pass type IV, multipass, lipid-anchor, GPI-anchor, peripheral membrane protein. With the avalanche of newly found protein sequences in the post-genomic age, it is urgent to develop an automatic and effective computational method to rapid and reliable prediction of the types of membrane proteins. At present, most of the existing methods were based on the assumption that one membrane protein only belongs to one type. Actually, a membrane protein may simultaneously exist at two or more different functional types. In this study, a new method by hybridizing the pseudo amino acid composition with multi-label algorithm called LIFT (multi-label learning with label-specific features) was proposed to predict the functional types both singleplex and multiplex animal membrane proteins. Experimental result on a stringent benchmark dataset of membrane proteins by jackknife test show that the absolute-true obtained was 0.6342, indicating that our approach is quite promising. It may become a useful high-through tool, or at least play a complementary role to the existing predictors in identifying functional types of membrane proteins.
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Efficient production of flavonoids in Fagopyrum tataricum hairy root cultures with yeast polysaccharide elicitation and medium renewal process.
Pharmacogn Mag
PUBLISHED: 07-24-2014
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Tartary buckwheat (Fagopyrum tataricum), an excellent edible and medicinal crop, has been widely used as a daily diet and traditional medicine for a long time. The major functional components of Fagopyrum tataricum have been demonstrated to be flavonoids (i.e. rutin and quercetin), which had notable andioxidant, antidiabetic, hypocholesterolemic and antitumor activities. Hairy root culture is a convenient and efficient plant tissue culture system for large scale production of bioactive metabolites.
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[Effect of blood activating water relieving method on heart functions and serum levels of NT-proBNP in patients with heart failure with normal ejection fraction].
Zhongguo Zhong Xi Yi Jie He Za Zhi
PUBLISHED: 03-29-2014
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To study the effect of blood activating water relieving method (BAWRM) on heart functions and serum levels of NT-proBNP in patients with heart failure with normal ejection fraction (HFNEF).
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A20 overexpression inhibits lipopolysaccharide-induced NF-?B activation, TRAF6 and CD40 expression in rat peritoneal mesothelial cells.
Int J Mol Sci
PUBLISHED: 02-11-2014
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Zinc finger protein A20 is a key negative regulator of inflammation. However, whether A20 may affect inflammation during peritoneal dialysis (PD)-associated peritonitis is still unclear. This study was aimed to investigate the effect of A20 overexpression on lipopolysaccharide (LPS)-induced inflammatory response in rat peritoneal mesothelial cells (RPMCs). Isolated and cultured RPMCs in vitro. Plasmid pGEM-T easy-A20 was transfected into RPMCs by Lipofectamine™2000. The protein expression of A20, phospho-I?B?, I?B?, TNF receptor-associated factor (TRAF) 6 and CD40 were analyzed by Western blot. The mRNA expression of TRAF6, CD40, interleukin-6 (IL-6) and tumor necrosis factor-? (TNF-?) were determined by real time-PCR. NF-?B p65 DNA binding activity, IL-6 and TNF-? levels in cells culture supernatant were determined by ELISA. Our results revealed that RPMCs overexpression of A20 lead to significant decrease of LPS-induced I?B? phosphorylation and NF-?B DNA binding activity (all p<0.01). In addition, A20 also attenuated the expression of TRAF6, CD40, IL-6 and TNF-? as well as levels of IL-6 and TNF-? in cells culture supernatant (all p<0.05). However, A20 only partly inhibited CD40 expression. Our study indicated that A20 overexpression may depress the inflammatory response induced by LPS in cultured RPMCs through negatively regulated the relevant function of adaptors in LPS signaling pathway.
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Improving the performance of protein kinase identification via high dimensional protein-protein interactions and substrate structure data.
Mol Biosyst
PUBLISHED: 01-21-2014
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As a crucial post-translational modification, protein phosphorylation regulates almost all basic cellular processes. Recently, thousands of phosphorylation sites have been discovered by large-scale phospho-proteomics studies, but only about 20% of them have information regarding catalytic kinases, which brings a great challenge for correct identification of the protein kinases responsible for experimentally verified phosphorylation sites. In most existing identification tools, only a local sequence was selected to construct predictive models, and information regarding protein-protein interaction (PPI) was adopted for further filtering. However, the limited information utilized by these tools is not sufficient to identify protein kinases responsible for phosphorylated proteins. In this work, a novel computational approach that fully incorporates PPI and substrate structure information is proposed to improve the performance of human protein kinase identification. To handle the issue of high-dimensional PPI and structure data, a two-step feature selection algorithm that incorporates a support vector machine (SVM), is designed to detect information useful in discriminating the corresponding kinase of phosphorylation sites. Benchmark datasets for kinase identification are constructed using human protein phosphorylation data extracted from the latest Phospho.ELM database. With the selected PPI and structure features, the performance of kinase identification is significantly enhanced as compared with that obtained by using only sequence information. To further verify our method, we compared it with the state-of-the-art tools NetworKIN and IGPS at two stringency levels with medium (>90.0%) and high (>99.0%) specificity. The results show that our method outperforms existing tools in identifying protein kinases. Further evaluation demonstrates that our method also has superior performance on different hierarchical levels including kinase, subfamily, family and group.
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Malignant mixed epithelial and stromal tumor of the kidney: the second male case and review of literature.
Int J Clin Exp Pathol
PUBLISHED: 01-01-2014
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Mixed epithelial and stromal tumor of the kidney is a recently described neoplasm that predominantly affects perimenopausal women. Although typically benign, few cases with malignant features have been reported. Here, we report the second male case of malignant mixed epithelial and stromal tumor of the kidney with sarcomatous transformation. The patient presented with abdominal discomfort and right flank pain. Computed tomography (CT) of the abdomen revealed a large mass arisen from the right kidney with solid and focal cystic components. The patient underwent right radical nephrectomy. Histologic sections showed benign and malignant components. The benign component consisted of multiple tubules and variably sized cysts lined by benign epithelium. The malignant component was predominantly composed of undifferentiated cellular spindle cell sarcoma. By immunohistochemical studies, the epithelial component was positive for cytokeratins and epithelial membrane antigen (EMA). The stromal component displayed strong immunohistochemical expression of vimentin, CD-99, bcl-2; and was negative for cytokeratins, desmin, SMA, S-100, estrogen receptor (ER) and progesterone receptor (PR). Analysis by reverse transcriptase polymerase chain reaction (RT-PCR) failed to identify the SYT-SSX1 or SYT-SSX2 fusion transcripts characteristic of synovial sarcoma. Subsequently adjuvant chemotherapy was given. The patient developed a local recurrent tumor 9 months after operation.
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[Expression and localization of vascular endothelial growth factor in the radial artery of the coronary artery bypass grafting patients with diabetes].
Zhonghua Wai Ke Za Zhi
PUBLISHED: 11-22-2013
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To evaluate the quality of the radial artery for coronary artery bypass grafting (CABG) from patients with diabetes by observing the morphology of the radial artery and detecting the expression of vascular endothelial growth factor (VEGF) which may attribute to the long-term patency rate of the coronary artery bypass grafting.
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Three new iridoid glycosides from the fruit of gardenia jasminoides var. radicans.
Chem. Pharm. Bull.
PUBLISHED: 10-04-2013
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Three new iridoid glycosides, 6?-O-trans-feruloylgenipin gentiobioside (1), 2-O-trans-p-coumaroylgardoside (2), 2-O-trans-feruloylgardoside (3), were isolated from the fruit of Gardenia jasminoides var. radicans MAKINO (Rubiaceae). The structures of these compounds were elucidated on the basis of MS, NMR spectra analysis, glycoside hydrolysis, and sugar derivatization coupled with HPLC analysis.
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[Effect of subanaesthetic dose of ketamine on mechanical stimulus on brain regions].
Zhongguo Yi Xue Ke Xue Yuan Xue Bao
PUBLISHED: 08-31-2013
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To investigate the effect of subanaesthetic dose of ketamine on mechanical stimulus on brain regions.
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Response surface modeling and optimization of ultrasound-assisted extraction of three flavonoids from tartary buckwheat (Fagopyrum tataricum).
Pharmacogn Mag
PUBLISHED: 06-11-2013
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Buckwheat (Fagopyrum spp., Polygonaceae) is a widely planted food crop. Flavonoids, including quercetin, rutin, and kaempferol, are the main bioactive components in tartary buckwheat (Fagopyrum tataricum (L.) Gaertn). From the nutriological and pharmacological perspectives, flavonoids have great value in controlling blood glucose and blood pressure levels, and they also have antioxidant properties.
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[Role of target controlled infusion of remifentanil for the prevention of etomidate induced myoclonus during general anesthesia].
Zhongguo Yi Xue Ke Xue Yuan Xue Bao
PUBLISHED: 04-23-2013
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To explore the preventive effect of etomidate-induced myoclonus by different concentrations of target-controlled infusion of remifentanil.
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The expression and functionality of stromal caveolin 1 in human adenomyosis.
Hum. Reprod.
PUBLISHED: 02-26-2013
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What is the expression pattern and functionality of caveolin 1 (CAV1) in the endometrium of patients with adenomyosis?
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Band filling and correlation controlling electronic properties and magnetism in K(x)Fe(2-y)Se2: a slave boson study.
J Phys Condens Matter
PUBLISHED: 02-19-2013
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We investigate the electronic and magnetic properties of K(x)Fe(2-y)Se2 materials at different band fillings utilizing the multi-orbital Kotliar-Ruckenstein slave boson mean-field approach. We find that the ground state of KFe2Se2 is a paramagnetic (PM) bad metal with intermediate correlation, in contrast with the previous antiferromagnetic (AFM) results obtained by the local density approximation. Our PM metallic ground state suggests that KFe2Se2 is the parent phase of superconducting K(x)Fe(2-y)Se2, supporting a recent scanning tunneling spectroscopy experiment. For pure Fe2+-based systems, the ground state is a striped AFM (SAFM) metal with a spin density wave gap partially opened near the Fermi level. In comparison, for Fe3+-based compounds, besides SAFM, a Néel AFM metal without orbital ordering is observed, and an orbital selective Mott phase (OSMP) accompanied by an intermediate-spin to high-spin transition is also found, giving a possible scenario of an OSMP in K(x)Fe(2-y)Se2. These results demonstrate that the band filling and correlation control the Fermi surface topology, electronic state and magnetism in K(x)Fe(2-y)Se2.
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Mild copper-mediated direct oxidative cross-coupling of 1,3,4-oxadiazoles with polyfluoroarenes by using dioxygen as oxidant.
Chemistry
PUBLISHED: 02-10-2013
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CH(3)CN and O(2) do the trick! A copper-mediated direct oxidative cross-coupling of 2-(het)aryl-1,3,4-oxadiazoles with polyhaloarenes under mild reaction conditions has been developed (see scheme). The process provides a concise access to biaryl structures containing polyhaloarenes, which are of interest in the fields of pharmaceuticals and functional materials. Acetonitrile and oxygen play crucial roles.
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Mechanistic insights into copper-catalyzed Sonogashira-Hagihara-type cross-coupling reactions: sub-mol% catalyst loadings and ligand effects.
Chemistry
PUBLISHED: 02-06-2013
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An efficient catalytic system for Sonogashira-Hagihara-type reactions displaying ligand acceleration in the copper-catalyzed formation of C(sp²)-C(sp) bonds is described. The structure of the ligand plays a key role for the coupling efficiency. Various copper sources show excellent catalytic activity, even in sub-mol% quantities. A wide variety of substituents is tolerated in the substrates. Mechanistic details have been revealed by kinetic measurements and DFT calculations.
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PKIS: computational identification of protein kinases for experimentally discovered protein phosphorylation sites.
BMC Bioinformatics
PUBLISHED: 01-25-2013
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Dynamic protein phosphorylation is an essential regulatory mechanism in various organisms. In this capacity, it is involved in a multitude of signal transduction pathways. Kinase-specific phosphorylation data lay the foundation for reconstruction of signal transduction networks. For this reason, precise annotation of phosphorylated proteins is the first step toward simulating cell signaling pathways. However, the vast majority of kinase-specific phosphorylation data remain undiscovered and existing experimental methods and computational phosphorylation site (P-site) prediction tools have various limitations with respect to addressing this problem.
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Rapid and simple method for the determination of emodin in tartary buckwheat (Fagopyrum tataricum) by high-performance liquid chromatography coupled to a diode array detector.
J. Agric. Food Chem.
PUBLISHED: 01-22-2013
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A simple and rapid method for determining emodin, an active factor presented in tartary buckwheat (Fagopyrum tataricum), by high-performance liquid chromatography coupled to a diode array detector (HPLC-DAD) has been developed. Emodin was separated from an extract of buckwheat on a Kromasil-ODS C(18) (250 mm × 4.6 mm × 5 ?m) column. The separation is achieved within 15 min on the ODS column. Emodin can be quantified using an external standard method detecting at 436 nm. Good linearity is obtained with a correlation coefficient exceeding 0.9992. The limit of detection and the limit of quantification are 5.7 and 19 ?g/L, respectively. This method shows good reproducibility for the quantification of the emodin with a relative standard deviation value of 4.3%. Under optimized extraction conditions, the recovery of emodin was calculated as >90%. The validated method is successfully applied to quantify the emodin in tartary buckwheat and its products.
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Reinforced aortic root reconstruction for acute type A aortic dissection involving the aortic root.
Rev Bras Cir Cardiovasc
PUBLISHED: 01-14-2013
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There are debates regarding the optimal approach for AAAD involving the aortic root. We described a modified reinforced aortic root reconstruction approach for treating AAAD involving the aortic root.
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The study of absorption kinetics of berberine based on portal vein in rat, and the influence of verapamil and borneol to its absorption ability by UHPLC method.
Eur J Drug Metab Pharmacokinet
PUBLISHED: 01-08-2013
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This paper aims to investigate the portal-vein absorption kinetics of berberine in rat, and the influence of P-gp inhibitors such as verapamil and borneol, to its absorption ability. In the paper, a validated UHPLC method was established to determine the berberine in plasma, and the portal-vein absorption model was applied to conduct the pharmacokinetic study. Animals were divided into four groups as follow: group berberine group (BG); group verapamil + berberine group (VBG); group borneol + berberine group (BBG) and group long-term use of borneol + berberine group (LBBG). Plasma samples were obtained at regular time intervals after administration and separated on Agilent 1290 Infinity UHPLC instrument. The method showed good linearity (r = 0.9988) over wide dynamic ranges (4.08-163.20 ng/mL). Variations within- and between-batch never exceeded 7.58 and 2.21 %, respectively. The extraction recovery rates ranged from 85.34 to 111.62 %. We discovered that the AUC of four group exhibited no significant differences (P > 0.05), and co-administration of berberine with borneol of group BBG and group LBBG in advance could both reduce the T max and C max as compared to group BG (P < 0.05), while co-administration of verapamil seems to have had little influence to berberines absorption ability.
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MicroRNA-21 and risk of severe acute kidney injury and poor outcomes after adult cardiac surgery.
PLoS ONE
PUBLISHED: 01-01-2013
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Severe acute kidney injury (AKI) after cardiac surgery is associated with poor clinical outcomes. This study evaluated the potential use of miR-21 as a risk marker for postoperative AKI progression and other poor outcomes.
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Cell death pathways in astrocytes with a modified model of oxygen-glucose deprivation.
PLoS ONE
PUBLISHED: 01-01-2013
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Traditional oxygen-glucose deprivation (OGD) models do not produce sufficiently stable and continuous deprivation to induce cell death in the ischemic core. Therefore, we modified the OGD model to mimic the observed damage in the ischemic core following stroke and utilized this new model to study cell death pathways in astrocytes. The PO2 and pH levels in the astrocyte culture medium were compared between a physical OGD group, a chemical OGD group and a mixed OGD group. The mixed OGD group was able to maintain anaerobic conditions in astrocyte culture medium for 6 h, while the physical and the chemical groups failed to maintain such conditions. Astrocyte viability decreased and LDH release into in the medium increased as a function of exposure to OGD. Compared to the control group, the expression of active caspase-3 in the mixed OGD group increased within 2 h after OGD, but decreased after 2 h of OGD. Additionally, porimin mRNA levels did not significantly increase during the first 2 h of OGD, while bcl-2 mRNA levels decreased at 1 h. However, both porimin and bcl-2 mRNA levels increased after 2 h of OGD; interestingly, they both suddenly decreased at 4 h of OGD. Taken together, these results indicate that apoptosis and oncosis are the two cell death pathways responsible for astrocyte death in the ischemic core. However, the main death pathway varies depending on the OGD period.
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[Effects of nitrogen fertilization, soil moisture and soil temperature on soil respiration during summer fallow season].
Huan Jing Ke Xue
PUBLISHED: 10-31-2011
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On the loess plateau, summer fallow season is a hot rainy time with intensive soil microbe activities. To evaluate the response of soil respiration to soil moisture, temperature, and N fertilization during this period is helpful for a deep understanding about the temporal and spatial variability of soil respiration and its impact factors, then a field experiment was conducted in the Changwu State Key Agro-Ecological Experimental Station, Shaanxi, China. The experiment included five N application rates: unfertilized 0 (N0), 45 (N45), 90 (N90), 135(N135), and 180 (N180) kg x hm(-2). The results showed that at the fallow stage, soil respiration rate significantly enhanced from 1.24 to 1.91 micromol x (m2 x s)(-1) and the average of soil respiration during this period [6.20 g x (m2 x d)(-1)] was close to the growing season [6.95 g x (m2 x d)(-1)]. The bivariate model of soil respiration with soil water and soil temperature was better than the single-variable model, but not so well as the three-factor model when explaining the actual changes of soil respiration. Nitrogen fertilization alone accounted for 8% of the variation soil respiration. Unlike the single-variable model, the results could provide crucial information for further research of multiple factors on soil respiration and its simulation.
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[Perioperative monitoring and control of hyperglycemia during deep hypothermic circulatory arrest].
Zhongguo Wei Zhong Bing Ji Jiu Yi Xue
PUBLISHED: 07-27-2011
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To observe the trend of change in perioperative blood glucose level in patients undergoing deep hypothermic circulatory arrest (DHCA), in order to evaluate the influencing factors of inciting hyperglycemia and the clinical effects of insulin control.
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[Comparative study on the ultrastructure of radial artery in elderly patients underwent coronary artery bypass grafting with diabetes mellitus].
Zhonghua Wai Ke Za Zhi
PUBLISHED: 06-29-2011
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To explore the impact of age and diabetes mellitus (DM) on the ultrastructure of radial artery (RA).
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Leaf rolling controlled by the homeodomain leucine zipper class IV gene Roc5 in rice.
Plant Physiol.
PUBLISHED: 05-19-2011
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Leaf rolling is considered an important agronomic trait in rice (Oryza sativa) breeding. To understand the molecular mechanism controlling leaf rolling, we screened a rice T-DNA insertion population and isolated the outcurved leaf1 (oul1) mutant showing abaxial leaf rolling. The phenotypes were caused by knockout of Rice outermost cell-specific gene5 (Roc5), an ortholog of the Arabidopsis (Arabidopsis thaliana) homeodomain leucine zipper class IV gene GLABRA2. Interestingly, overexpression of Roc5 led to adaxially rolled leaves, whereas cosuppression of Roc5 resulted in abaxial leaf rolling. Bulliform cell number and size increased in oul1 and Roc5 cosuppression plants but were reduced in Roc5-overexpressing lines. The data indicate that Roc5 negatively regulates bulliform cell fate and development. Gene expression profiling, quantitative polymerase chain reaction, and RNA interference (RNAi) analyses revealed that Protodermal Factor Like (PFL) was probably down-regulated in oul1. The mRNA level of PFL was increased in Roc5-overexpressing lines, and PFL-RNAi transgenic plants exhibit reversely rolling leaves by reason of increases of bulliform cell number and size, indicating that Roc5 may have a conserved function. These are, to our knowledge, the first functional data for a gene encoding a homeodomain leucine zipper class IV transcriptional factor in rice that modulates leaf rolling.
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Pharmacokinetic profile of total quercetin after single oral dose of tartary buckwheat extracts in rats.
J. Agric. Food Chem.
PUBLISHED: 03-18-2011
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As an important edible and medicinal material, tartary buckwheat ( Fagopyrum tataricum Gaertn.) is commonly used as a kind of food or drug in eastern Asian countries. To investigate the pharmacokinetic profile of total quercetin after a single oral dose of tartary buckwheat extract in rats, a sensitive, simple, and accurate HPLC-UV method was developed to determine total quercertin following plasma enzyme hydrolysis with glucuronidase/sulfatase. Eighteen male Wistar rats were randomly assigned into three groups, which were given three different doses of tartary buckwheat extract. The pharmacokinetic results showed that the area under the plasma concentration-time curve (AUC) of total quercetin after single oral doses of tartary buckwheat extract presented a linear relationship. The peak plasma concentrations (C(max)) of total quercetin afte plasma enzyme hydrolysis with glucuronidase/sulfatase were 0.55 ± 0.26, 1.10 ± 0.53, and 2.05 ± 0.26 ?g/mL; the peak times (T(max)) were 2.33 ± 1.94, 2.75 ± 3.67, and 2.50 ± 1.82 h; the areas under the curves (AUC(0-36h)) were 5.29 ± 1.35, 10.02 ± 4.43, and 22.51 ± 3.05 ?g·mL(-1)·h(-1) for three doses of tartary buckwheat extract (60, 120, and 240 mg/kg). The present study has provided a basic pharmacokinetic profile of total quercetin after a single oral dose of tartary buckwheat extract in rats.
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[Retrospective study on Chinese medicine syndrome patterns and their associated factors in patients with type 2 diabetic nephropathy stage III].
Zhongguo Zhong Xi Yi Jie He Za Zhi
PUBLISHED: 12-25-2010
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To explore the relationship between Chinese medicine syndrome patterns (CMSP) and their associated factors in patients with type 2 diabetic nephropathy stage III (DN2-3).
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[Relative factors in home safety evaluated by multilevel statistical models].
Zhonghua Liu Xing Bing Xue Za Zhi
PUBLISHED: 12-18-2010
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To understand the situations of home establishments and the related domestic behaviors that causing injuries.
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[Mechanism involving blm gene underlies repair of DNA damage of Jurkat cells induced by mitomycin C].
Zhongguo Shi Yan Xue Ye Xue Za Zhi
PUBLISHED: 12-07-2010
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The defect or block of apoptosis is an important factor involved in the drug resistance of tumor cells. Blm gene plays a great role in DNA damage and repair. This study was aimed to explore the relationship of blm gene expression with cell cycle and apoptosis after Jurkat DNA damage. The apoptosis rate and change of cell cycle were detected by flow cytometry, the expression level of blm mRNA in Jurkat cells was determined by semi-quantitative RT-PCR. The results indicated that after induction with 0.4 g/L of mitomycin C (MMC) for 24 hours the apoptosis rate of Jurkat cells were (11.42±0.013)%, and (66.08±1.60)% Jurkat cells were arrested in G2/M phase. After induction for 48 hours, the apoptosis rate of Jurkat cells declined from (11.42±0.013)% to (8.08±0.27)%, and cell count of Jurkat cells arrested in G2/M phase decreased from (66.08±1.60)% to (33.96±1.05)%. When induced with 0.4 g/L of MMC for 24 hours, the apoptosis rate of fibroblasts and the percentage of fibroblasts in G2/M, G0-G1 and S phase all showed no significant change until 48 hours. The range of apoptosis rate and the change of cell percentage in three phases were significantly different between Jurkat cells and fibroblasts (p<0.01). Expression level of blm mRNA in Jurkat cells was remarkably higher than that in normal fibroblasts (p<0.01), at 48 hours expression level of blm mRNA was remarkably higher than that at 24 hours. The 2 groups showed clear difference of blm mRNA expression after treated by MMC (p<0.01). It is concluded that the blm gene may play a significant role in repair of DNA damage of Jurkat cells after MMC induction. Abnormal expression of blm is correlated to the drug resistance of leukemia cells.
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[Surgical treatment of congenital quadricuspid aortic valve].
Zhonghua Yi Xue Za Zhi
PUBLISHED: 11-13-2010
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To improve the understanding of congenital quadricuspid aortic valve (QAV), explore its echocardiographic diagnostic value and summarize the methods and outcomes of surgical treatments.
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[Reoperative valve replacement in patients undergoing cardiac reoperation: a report of 104 cases].
Zhonghua Wai Ke Za Zhi
PUBLISHED: 11-09-2010
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To review the experience of reoperative valve replacement for 104 patients.
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Orbital polarizations and superconducting phase diagrams in two-orbital asymmetric t-J models.
J Phys Condens Matter
PUBLISHED: 08-13-2010
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We present the zero-temperature superconducting (SC) phase diagrams of the two-orbital asymmetric t-J model on a square lattice by means of the auxiliary-boson approach. Besides the two-gap SC and normal phases, we find an orbital dependent intermediate SC phase, in which one orbit is fully SC and another orbit is exponentially vanishing gapped SC. Such an intermediate phase is stable only for sufficient asymmetry in orbital space and doping concentration. The implication of the present scenario on the iron-based and other multi-orbital superconductors is discussed.
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[An analysis of the risk factors for the severity of paralysis in patients with hypokalemic periodic paralysis].
Zhonghua Nei Ke Za Zhi
PUBLISHED: 05-11-2010
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To analyze the independent factors for the severity of paralysis in the patients with hypokalemic periodic paralysis (HOPP).
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Role of prostaglandin E and its receptors in the process of ductus arteriosus maturation and functional closure in the rabbit.
Clin. Exp. Pharmacol. Physiol.
PUBLISHED: 01-17-2010
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1. Patent ductus arteriosus (PDA) is a common congenital heart defect in premature infants. The present study was designed to determine the role of the prostaglandin (PG) E(2) pathway in the process of ductus arteriosus (DA) maturation and functional closure. 2. Changes in PGE(2) pathway-related enzymes and receptors in DA in preterm and term rabbits were examined at both the mRNA and protein levels. In addition, responses of DA rings to Po(2) and PGE(2) were determined. 3. Circulating PGE(2) levels remained high until 2 h after birth. High levels of the EP(4) receptor were found in preterm DA. These tissues were sensitive to PGE(2), which caused vessel dilation, but were insensitive to increased Po(2). In contrast, DA tissues from term rabbits exhibited an immediate contractile response to increased Po(2) and PGE(2) treatment resulted in vasoconstriction, which was associated with increased EP(3) and decreased EP(4) receptor expression in term DA. 4. In conclusion, the preterm PDA is maintained by high levels of PGE(2), which mainly binds to the EP(4) receptor under conditions of hypoxia. In contrast, in the term DA, in which levels of the EP(3) receptor are higher than in preterm DA, exposure to PGE(2) resulted in vasoconstriction under normoxic conditions. These findings suggest that blocking the EP(4) receptor may represent a more selective treatment for the preterm PDA, whereas activating the EP(3) receptor may be more suitable for the treatment of the term PDA.
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Association of single-nucleotide polymorphisms in toll-like receptor 5 gene with rheumatic heart disease in Chinese Han population.
Int. J. Cardiol.
PUBLISHED: 06-22-2009
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This case-control study was carried out to investigate the association of single-nucleotide polymorphisms (SNPs) in toll-like receptor 5 (TLR5) gene and rheumatic heart disease (RHD) among the Chinese Han population. A group of 239 patients with RHD and 478 healthy controls were studied. Three tag single-nucleotide polymorphisms (tSNPs) in TLR5 gene (rs1640827, rs2353476, and rs5744140) were identified with microarray hybridization analysis. Significant differences were observed in the distribution of alleles and genotypes between the patients with RHD and the controls for either rs1640827 or rs2353476. Patients with RHD also have higher frequencies of CGC and CAC haplotypes. These findings indicate that the genetic variants of TLR5 gene might be associated with RHD in Chinese Han population.
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Superconducting pairing symmetry and energy gaps of the two-orbital t-t-J-J model: comparisons with the ARPES experiments in iron pnictides.
J Phys Condens Matter
PUBLISHED: 05-27-2009
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Motivated by the discovery of the iron-based superconductors, we present the theoretical results on the superconducting phase diagram, the temperature-dependent Fermi surface structures in normal state and the angle-resolved photoemission spectroscopy (ARPES) character of quasiparticles of the two-orbital t-t-J-J model. In the reasonable physical parameter region of LaFeAsO(1-x)F(x), we find the superconducting phase is stable, and the pairing symmetry is weakly anisotropic and nodeless d(x(2)-?y(2))+S(x(2)y(2))-wave, qualitatively in agreement with the ARPES experiments in iron pnictide superconductors. Nevertheless, the two ratios of the energy gaps to T(c) deviate from the ARPES data, suggesting that a more elaborate theoretical model is needed.
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Charge and spin orderings in triangular t-J-V model with quarter filling: application to Na(0.5)CoO(2).
J Phys Condens Matter
PUBLISHED: 04-24-2009
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We investigated the ground state (GS) properties of the single-orbital t-J-V model in a quarter-filling two-dimensional triangular lattice with the slave-boson mean-field approach. We found that the charge ordering (CO) and spin ordering arising from the spin exchange interaction J competes with that from the inter-site Coulomb interaction V. For comparatively large J, the stable GS is insulating with striped CO and antiferromagnetic ordering, avoiding the homogeneous frustrated phase. Accompanying the insulating CO phase, a small insulating gap is opened. Within a reasonable J and V parameter region, our results are consistent with the recent neutron scattering experiments in Na(0.5)CoO(2), which possibly elucidate its CO, magnetic and other GS properties.
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[Psychedelic effects of subanesthetic doses of ketamine].
Zhongguo Yi Xue Ke Xue Yuan Xue Bao
PUBLISHED: 03-26-2009
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To study the psychedelic effects in healthy volunteers when given subanesthetic dose of ketamine.
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Surgical treatment of multivalvular endocarditis: twenty-one-year single center experience.
J. Thorac. Cardiovasc. Surg.
PUBLISHED: 03-17-2009
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Little information is available about surgical outcomes in patients with multivalvular endocarditis. The aim of this article is to review the 21-year experience with surgical treatment of patients with multivalvular endocarditis at our institution and, in particular, to determine the incidence, pathologic status, diagnosis, surgical strategies, and outcomes of patients with this disease.
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Anharmonic effect on lattice distortion, orbital ordering and magnetic properties in Cs(2)AgF(4).
J Phys Condens Matter
PUBLISHED: 01-14-2009
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We develop the cluster self-consistent field method incorporating both electronic and lattice degrees of freedom to study the origin of ferromagnetism in Cs(2)AgF(4). After self-consistently determining the harmonic and anharmonic Jahn-Teller distortions, we show that the anharmonic distortion stabilizes the staggered x(2)-z(2)/y(2)-z(2) orbital and ferromagnetic ground state, rather than the antiferromagnetic one. The amplitudes of lattice distortions, Q(2) and Q(3), the magnetic coupling strengths, J(x,y), and the magnetic moment are in good agreement with the experimental observations.
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[Effect of borneol on intestinal absorption of muscone in rats].
Zhongguo Zhong Yao Za Zhi
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To explore the influence of borneol on intestinal absorption of muscone in rats.
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[Methods and results of surgical treatment for aortic root pathology due to Stanford A aortic dissection].
Zhonghua Wai Ke Za Zhi
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To investigate the effectiveness of surgical approaches, outcomes and prognosis of aortic root pathology due to Stanford A aortic dissection.
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[Determination method of muscone in rat intestinal perfusate by GC-MS/MS and its intestinal absorption kinetic characteristics in rats].
Zhongguo Zhong Yao Za Zhi
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To establish the method for determining muscone in rat intestinal perfusate by GC-MS/MS and study its intestinal absorption kinetic characteristics in rats.
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A local risk prediction model for prolonged ventilation after adult heart valve surgery in a Chinese single center.
Heart Lung
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The aim of this study was to develop a logistic risk prediction model for prolonged ventilation after adult heart valve surgery.
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Transition metal-free direct C-H bond thiolation of 1,3,4-oxadiazoles and related heteroarenes.
Chem. Commun. (Camb.)
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A convenient transition metal-free procedure for the direct thiolation of 1,3,4-oxadiazole C-H bonds using diaryl disulfides has been developed. Other substrates including indole, benzothiazole, N-phenylbenzimidazole, and caffeine were also thiolated in this manner, providing the corresponding products in good to excellent yields.
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Effects of yeast polysaccharide on growth and flavonoid accumulation in Fagopyrum tataricum sprout cultures.
Molecules
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The purpose of this study was to investigate the effects of yeast polysaccharide (YPS) on growth and flavonoid accumulation in sprout cultures of Fagopyrum tataricum (tartary buckwheat). Without obvious change in the appearance of the sprouts, the exogenous YPS notably stimulated the production of functional metabolites in F. tataricum sprouts, and the stimulation effect was concentration-dependent. With 400 mg/L of YPS applied to the sprout cultures on day 6, the total rutin and quercentin content was effectively increased to 42.8 mg/gdw, or about 1.4-fold in comparison with the control of 31.2 mg/gdw. Feeding with 800 mg/L of YPS on day 9, the sprouts biomass was increased by about 8% compared to the control culture (0.99 gdw/100 sprouts versus 0.92 gdw/100 sprouts). Moreover, the present study revealed that the accumulation of these bioactive metabolites resulted from the stimulation of the phenylpropanoid pathway by YPS treatment. It could be an effective strategy for improving the functional quality of the F. tataricum sprouts provided with YPS.
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Comparative study on the histomorphology and molecular biology of radial artery conduits in patients with diabetes mellitus who underwent coronary bypass surgery.
Diab Vasc Dis Res
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We studied the impact of diabetes mellitus (DM) on the radial artery (RA) in 30 patients with DM and 30 non-diabetic patients undergoing coronary artery bypass grafting with autologous RA. RAs were recorded as normal if there was no cellular or stromal tissue between the endothelium and the internal elastic lamina. The RA was normal in 26.7% of diabetic and 76.7% of non-diabetic patients (p = 0.000298). Intimal thickness index and intima:media ratio were higher in the former than in the latter (p < 0.05; p < 0.05), with no significant difference in luminal narrowing (p > 0.05). Electron microscopy scores were lower in the non-diabetic group (p < 0.001); endothelial nitric oxide synthase (eNOS) protein expression and optical density were higher (p < 0.001). Von Willebrand factor and endothelin-1 messenger RNA (mRNA) levels were higher in the DM patients (p < 0.001). The quality of the RA in patients with DM was thus inferior to that in non-diabetic patients. Care should be taken when selecting RA as a conduit in patients with DM.
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Risk factors for postoperative recurrence of cardiac myxoma and the clinical managements: a report of 5 cases in one center and review of literature.
Chin. Med. J.
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Recurrence or metastasis of myxomas is not rare and can lead to malignancy. We aimed to analyze the risk factors for postoperative cardiac myxoma recurrence and to summarize its clinical characteristics, treatments and classification.
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A genome-wide association study of Alzheimers disease using random forests and enrichment analysis.
Sci China Life Sci
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Alzheimers disease (AD) is a serious neurodegenerative disorder and its cause remains largely elusive. In past years, genome-wide association (GWA) studies have provided an effective means for AD research. However, the univariate method that is commonly used in GWA studies cannot effectively detect the biological mechanisms associated with this disease. In this study, we propose a new strategy for the GWA analysis of AD that combines random forests with enrichment analysis. First, backward feature selection using random forests was performed on a GWA dataset of AD patients carrying the apolipoprotein gene (APOE?4) and 1058 susceptible single nucleotide polymorphisms (SNPs) were detected, including several known AD-associated SNPs. Next, the susceptible SNPs were investigated by enrichment analysis and significantly-associated gene functional annotations, such as alternative splicing, glycoprotein, and neuron development, were successfully discovered, indicating that these biological mechanisms play important roles in the development of AD in APOE?4 carriers. These findings may provide insights into the pathogenesis of AD and helpful guidance for further studies. Furthermore, this strategy can easily be modified and applied to GWA studies of other complex diseases.
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Effects of propofol on brain activation in respond to mechanical stimuli.
Zhongguo Yi Xue Ke Xue Yuan Xue Bao
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To observe the effects of different concentrations of propofol on brain regions activated by mechanical stimuli, and then to investigate the analgesic effect of propofol.
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[Study on the unintended pregnancy among married women of child-bearing age living in Qingshan district, Wuhan].
Zhonghua Liu Xing Bing Xue Za Zhi
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To investigate the prevalence of unintended pregnancy (UP) and exploring the risk factors of UP for married women of child-bearing age from Qingshan district, Wuhan.
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Decreased and inactivated nuclear factor kappa B 1 (p50) in human degenerative calcified aortic valve.
Cardiovasc. Pathol.
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Degenerative aortic valve calcification is an important factor in aortic stenosis and incompetence, but the pathogenesis is unclear. The purpose of the present study was to observe the expression of p50 in degenerative calcified aortic valves, which may provide a potential therapeutic target.
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Metabolic profiling assisted quality assessment of Rhodiola rosea extracts by high-performance liquid chromatography.
Planta Med.
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In this work, fast and sensitive high-performance liquid chromatography (HPLC) coupled with multivariate analysis was utilized to assist the quality assessment of Rhodiola rosea extracts (RREs). 131 peaks were separated and detected in RREs on a fused-core C18 column. Principal component analysis (PCA) and hierarchical cluster analysis (HCA) of the chromatographic data demonstrated that 10 batches of RREs could be well-differentiated and categorized into three groups which were closely related to the origins of RREs. Partial least square-discriminant analysis (PLS-DA) showed that the quality differentiation might be explained by at least 6 components, in which rosavin was characterized by an external reference, rosiridine was identified by liquid chromatography-mass spectrometry (LC-MS), and the mass spectra of the others were provided. The observation that the level of rosavin was more relevant to the multivariate chromatographic data than the ones of salidroside and tyrosol, the other two components commonly used to standardize RREs, was confirmed by the PLS prediction models. Results of the present study not only indicated that rosavin was a rational marker to represent the quality of RREs, but also demonstrated the power of HPLC-based metabolic profiling in the quality assessment of herbal extracts.
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High level expression of bikunin in Pichia pastoris by fusion of human serum albumin.
AMB Express
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Bikunin is a proteoglycan exhibiting broad-spectrum inhibitory activity against serine proteases and could potentially suppress tumor cell invasion and metastasis. Here, we have successfully expressed recombinant human bikunin (rh-bikunin) in Pichia pastoris and also established the purification procedure. Different fusion genes of h-UTI and domain I, domain I and domain II, domain I, domain II and domain III of human serum albumin (HSA) were inserted into expression vector pPICZ?A. After expressed in shake flask, rh-bikunin was produced in an 30-L fermenter and purified by affinity chromatography and cation exchange chromatography. The final expression levels were 200 mg/L and we got totally 1.08 g (3650 IU/mg) of active purified rh-bikunin (purity is 98%) from 20 L of fermentation broth. The rh-bikunin consists of unique form with molecular masses of 25 kDa, and has the same N-terminals sequence as human native bikunin. This study provided a new method for high level expression of active rh-bikunin by using HSA as fusion parter.
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Relation between zinc, copper, and magnesium concentrations following cardiopulmonary bypass and postoperative atrial fibrillation in patients undergoing coronary artery bypass grafting.
Biol Trace Elem Res
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Atrial fibrillation is the most frequently encountered arrhythmia following cardiac surgery. Since the essential trace elements zinc, copper, and magnesium are suspected to have an effect on postoperative atrial fibrillation, the concentrations of these elements were determined by flame atomic absorption spectrophotometry in the plasma of 60 patients undergoing elective coronary artery bypass grafting. Blood samples were collected every 30 min during cardiopulmonary bypass and postoperatively. Plasma concentrations of copper, zinc, and magnesium were measured with flame atomic absorption spectrophotometry. All patients were monitored by continuous electrocardiography until they became outpatients or immediately after atrial fibrillation had taken place. Atrial fibrillation occurred in 13 of the 60 patients, corresponding to 21.7%. The zinc and copper concentrations at postoperative days 1 and 3 were significantly different (P < 0.05) between patients with and without atrial fibrillation. The concentrations of zinc following cardiopulmonary bypass recovered more slowly in patients with postoperative atrial fibrillation than in patients without it. Whether or not supplemental zinc could lower the incidence of postoperative atrial fibrillation should be evaluated in future prospective randomized clinical trials.
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Influence of electronic correlations on orbital polarizations in the parent and doped iron pnictides.
J Phys Condens Matter
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Orbital polarization and electronic correlation are two essential aspects in understanding the normal-state and superconducting properties of multi-orbital FeAs-based superconductors. In this paper, we present a systematic study on the orbital polarization of iron pnictides from weak to strong Coulomb correlations within the Kotliar-Ruckenstein slave boson approach. The magnetic phase diagram of the two-orbital model for LaFeAsO clearly shows that a striped antiferromagnetic metallic phase with orbital polarization exists over a wide doping range, in addition to the Slater-type insulator, Mott insulator and paramagnetic phases. A reversal of the orbital polarization occurs in the intermediate correlation regime in the absence of the crystal field splitting; however, a small crystal field splitting considerably enhances the orbital polarization, and stabilizes the xz-type orbital order. We argue that the ferro-orbital polarization is characteristic of a density wave, and leads to a pseudogap-like behavior in the density of states.
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ENHANCEMENT OF RUTIN PRODUCTION IN FAGOPYRUM TATARICUM HAIRY ROOT CULTURES WITH ITS ENDOPHYTIC FUNGAL ELICITORS.
Prep. Biochem. Biotechnol.
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Tartary buckwheat (Fagopyrum tataricum) is a potentially important source of rutin, a natural bioactive flavonoid with antihyperglycemic, antioxidative, antihypertensive and anti-inflammatory properties. This study was to examine the effects of endophytic fungi on rutin production in the hairy root cultures of F. tataricum. Without obvious changes in the appearance of the hairy roots, the exogenous fungal mycelia elicitors efficiently stimulated the hairy root growth and rutin biosynthesis, and the stimulation effect was mainly depending on the mycelia elicitor species, as well as its treatment dose. Two endophytic fungal isolates Fat9 (Fusarium oxysporum) and Fat15 (Alternaria sp.) were screened to be the promising candidates for promoting F. tataricum hairy root growth and rutin production. With application of polysaccharide (PS) of endophyte Fat9 (200 mg/L), and PS of endophyte Fat15 (100 mg/L) to the hairy root cultures on day 25, the rutin yield was increased to 45.9 mg/L and 47.2 mg/L, respectively. That was about 3.1- to 3.2-fold in comparison with the control of 14.6 mg/L. Moreover, the present study revealed the accumulation of rutin resulted from the stimulation of the phenylpropanoid pathway by mycelia PS treatments. It may be an efficient strategy for enhancing rutin production in F. tataricum hairy root culture provided with its endophytic mycelia elicitors.
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