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Find video protocols related to scientific articles indexed in Pubmed.
Chinese Herbal Medicine for Obesity: A Randomized, Double-Blinded, Multicenter, Prospective Trial.
Am. J. Chin. Med.
PUBLISHED: 11-20-2014
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Obesity is a serious medical problem worldwide. As a holistic therapy, traditional Chinese medicine (TCM) may have a potential in obesity management. In this controlled trial, we evaluated the safety and effectiveness of Xin-Ju-Xiao-Gao-Fang (XJXGF), a TCM herbal formulation, in 140 obese subjects over a 24-week period. The XJXGF formula mainly consists of rhubarb, coptis, semen cassia, and citrus aurantium. Subjects with body mass index (BMI) 28-40 kg/m(2) were recruited at 5 centers in China. We assessed the changes in subjects' body weight, its related parameters, and the reduction of insulin resistance (IR) after administration of XJXGF formula or low-dose XJXGF (10% of the XJXGF formula, as control). After 24-week treatment, among participants in the XJXGF formula group and low-dose XJXGF group, the mean ± SE changes in the body weight were -3.58±0.48 and -1.91±0.38 kg, respectively (p < 0.01). The changes in the IR-index of two groups were -2.65±1.04 and -1.58±1.3, respectively (p < 0.05). There were no serious adverse events reported during the 24-week trial. Participants reported 7 minor adverse events, 4 in the XJXGF formula group and 3 in the low-dose XJXGF group (p = 0.578). Future studies are needed to investigate the clinical utility of this TCM formulation in the treatment of obese subjects.
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The early diagnostic and prognostic utility of high-sensitive troponin assays in acute myocardial infarction: a meta-analysis.
Intern Med J
PUBLISHED: 11-12-2014
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To determine the diagnostic and prognostic utility of high-sensitive troponin assays in very early phase of AMI (less than 3 hours since symptoms onset) by performing a meta-analysis of prospective studies.
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Inappropriate left ventricular mass and poor outcomes in patients with angina pectoris and normal ejection fraction.
Coron. Artery Dis.
PUBLISHED: 11-06-2014
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Although inappropriate left ventricular mass has been associated with clustered cardiac geometric and functional abnormalities, its predictive value in patients with coronary artery disease is still unknown. This study examined the association of inappropriate left ventricular mass with clinical outcomes in patients with angina pectoris and normal ejection fraction.
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Engineered nanoparticles: thrombotic events in cancer.
Nanoscale
PUBLISHED: 10-28-2014
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Engineered nanoparticles are being increasingly produced for specific applications in medicine. Broad selections of nano-sized constructs have been developed for applications in diagnosis, imaging, and drug delivery. Nanoparticles as contrast agents enable conjugation with molecular markers which are essential for designing effective diagnostic and therapeutic strategies. Such investigations can also lead to a better understanding of disease mechanisms such as cancer-associated thrombosis which remains unpredictable with serious bleeding complications and high risk of death. Here we review the recent and current applications of engineered nanoparticles in diagnosis and therapeutic strategies, noting their toxicity in relation to specific markers as a target.
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Macular Measurements Using Spectral-Domain Optical Coherence Tomography in Chinese Myopic Children.
Invest. Ophthalmol. Vis. Sci.
PUBLISHED: 10-16-2014
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To evaluate the macular thickness/volume in Chinese myopic children using spectral-domain optical coherence tomography (SD-OCT) and assess its correlation with spherical equivalent refraction (SER), axial length (AL), sex, and age.
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[Macular thickness in unilateral amblyopia as measured by optical coherence tomography: a meta analysis].
Zhonghua Yan Ke Za Zhi
PUBLISHED: 10-15-2014
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To offer a clue for the etiology of amblyopia by systematically comparing the macular thickness measured by optical coherence tomography (OCT) between the amblyopic eye and the fellow normal eye in patients with unilateral amblyopia.
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[Clinical analysis of cervical screening in 2329 pregnant women].
Nan Fang Yi Ke Da Xue Xue Bao
PUBLISHED: 09-30-2014
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To study the cytopathologic characteristics of cervical diseases in pregnant women and the outcomes of the postpartum women to provide evidence for prevention and treatment of cervical cancer.
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Vascular channels formed by subpopulations of PECAM1(+) melanoma cells.
Nat Commun
PUBLISHED: 09-09-2014
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Targeting the vasculature remains a promising approach for treating solid tumours; however, the mechanisms of tumour neovascularization are diverse and complex. Here we uncover a new subpopulation of melanoma cells that express the vascular cell adhesion molecule PECAM1, but not VEGFR-2, and participate in a PECAM1-dependent form of vasculogenic mimicry (VM). Clonally derived PECAM1(+) tumour cells coalesce to form PECAM1-dependent networks in vitro and they generate well-perfused, vascular endothelial growth factor (VEGF)-independent channels in mice. The neural crest specifier AP-2? is diminished in PECAM1(+) melanoma cells and is a transcriptional repressor of PECAM1. Re-introduction of AP-2? into PECAM1(+) tumour cells represses PECAM1 and abolishes tube-forming ability, whereas AP-2? knockdown in PECAM1(-) tumour cells upregulates PECAM1 expression and promotes tube formation. Thus, VM-competent subpopulations, rather than all cells within a tumour, may instigate VM, supplant host-derived endothelium, and form PECAM1-dependent conduits that are not diminished by neutralizing VEGF.
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miRNA-646 suppresses osteosarcoma cell metastasis by downregulating fibroblast growth factor 2 (FGF2).
Tumour Biol.
PUBLISHED: 09-05-2014
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MicroRNAs are short regulatory RNAs that play crucial roles in cancer development and progression. MicroRNA-646 (miR-646) is downregulated in many human cancers, and increasing evidence indicates that it functions as a tumor suppressor. However, the role of miR-646 in osteosarcoma remains unclear. Expression levels of miR-646 in osteosarcoma cell lines and patient tissues were evaluated by quantitative real-time PCR (qRT-PCR), and the clinicopathological significance of the resultant data was later analyzed. Next, we investigated the role of miR-646 to determine its potential roles on osteosarcoma cell proliferation, migration, and invasion in vitro. A luciferase reporter assay was conducted to confirm the target gene of miR-646, and the results were validated in the osteosarcoma cell line. In this study, we found that miR-646 was downregulated in osteosarcoma cell lines and osteosarcoma tissues compared with normal osteoblast cell line NHOst and paired adjacent nontumor tissue. We found that a lower expression of miR-646 was associated with metastasis. In osteosarcoma cells, overexpression of miR-646 inhibited cell proliferation, migration, and invasion. In contrast, downregulation of miR-646 expression promoted osteosarcoma cell proliferation, migration, and invasion. Next, we identified that the FGF2 gene is a novel direct target of miR-646 in osteosarcoma cells. Moreover, enforced expression of FGF2 partially reversed the inhibition of cell proliferation, migration, and invasion that was caused by miR-646. Our study demonstrated that miR-646 might be a tumor suppressor in osteosarcoma via the regulation of FGF2, which provided a potential prognostic biomarker and therapeutic target.
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Changes of Serum Osteocalcin, Calcium, and Potassium in a Rat Model of Type 2 Diabetes.
Cell Biochem. Biophys.
PUBLISHED: 09-04-2014
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Changes in serum levels of calcium (Ca), phosphorus (P), Ca phosphate, alkaline phosphatase (AKP), and osteocalcin (BGP) were assessed in a model of type 2 diabetes in 8-weeks-old Sprague-Dawley rats. Throughout the study, the animals in Group A consumed high-carbohydrate, high-fat diet and were administered two intraperitoneal injections of streptozocin (STZ) at 4 (25 mg/kg) and 8 (40 mg/kg) weeks after diet initiation. Animals in Group B received similar diet but intraperitoneal injections with citrate buffer. The control group rats (Group C) were fed normal diet and received citrate buffer injections. The Ca levels in Group A were significantly decreased at 12 and 16 weeks, while P levels were significantly lower at 8, 12, and 16 weeks after the first STZ injection (p < 0.05 vs. Groups B and C). The Ca phosphate levels in Group A were significantly lower at 16 weeks (p < 0.05 vs. Groups B and C). There was a discrepancy in kinetics of AKP and BGP levels in Group A, such that the former showed a significant surge at 8 weeks (p < 0.05 vs. Groups B and C) followed by a decline at 16 weeks (p < 0.05 vs. Groups B and C), while the levels of the latter consistently decreased and became significantly decreased at 12 and 16 weeks (p < 0.05 vs. Groups B and C). In conclusion, these findings may lead to better understanding of the pathogenesis of osteoporosis in diabetic rats.
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[Zero-profile implant for anterior cervical discectomy and fusion in treating single cervical disc herniation].
Zhongguo Gu Shang
PUBLISHED: 08-30-2014
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To explore the efficacy and safety of zero-profile implant for anterior cervical discectomy and fusion (ACDF) in treating single cervical disc herniation.
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[Study on transition metals in airborne particulate matter in Shanghai city's subway].
Huan Jing Ke Xue
PUBLISHED: 08-28-2014
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PM10 and PM2.5 aerosol particle samples were collected at a subway station in Shanghai and their morphology, chemical composition and transition metal species were studied. The mass concentrations of PM10 and PM2.5 inside the subway station were significantly higher than those measured in aboveground ambient air. The PM levels inside subway were much higher than the state control limit. The aerosol composition in the metro station was quite different from that of the aboveground urban particles. Concentrations of Fe, Mn and Cr were higher than the averages of aboveground urban air particles by factors of 8, 2, and 2, respectively, showing a substantial enrichment in subway. Scanning electron microscope (SEM) analysis showed that the subway particles had flat surfaces in combination with parallel scratches and sharp edges and looked like metal sheets or flakes. Furthermore, analysis of the atomic composition of typical subway particles by energy dispersive X-Ray (EDX) spectroscopy showed that oxygen and iron dominated the mass of the particles. The X-ray absorption near-edge structure (XANES) spectroscopy results showed that a fraction (> 26%) of the total iron in the PM10 was in the form of pure Fe, while in the street particles Fe(III) was shown to be a significant fraction of the total iron. The work demonstrated that the underground subway stations in Shanghai were an important microenvironment for exposure to transition metal aerosol for the people taking subway train for commuting every day and those who work in the subway stations, and the metal particle exposure for people in the subway station should not be ignored.
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Microfluidic serpentine antennas with designed mechanical tunability.
Lab Chip
PUBLISHED: 08-22-2014
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This paper describes the design and characterization of microfluidic serpentine antennas with reversible stretchability and designed mechanical frequency modulation (FM). The microfluidic antennas are designed based on the Poisson's ratio of the elastomer in which the liquid alloy antenna is embedded, to controllably decrease, stabilize or increase its resonance frequency when being stretched. Finite element modelling was used in combination with experimental verification to investigate the effects of substrate dimensions and antenna aspect ratios on the FM sensitivity to uniaxial stretching. It could be designed within the range of -1.2 to 0.6 GHz per 100% stretch. When the aspect ratio of the serpentine antenna is between 1.0 and 1.5, the resonance frequency is stable under stretching, bending, and twisting. The presented microfluidic serpentine antenna design could be utilized in the field of wireless mobile communication for the design of wearable electronics, with a stable resonance frequency under dynamic applied strain up to 50%.
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More favorable response to cardiac resynchronization therapy in women than in men.
Circ Arrhythm Electrophysiol
PUBLISHED: 08-21-2014
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Data on sex difference in response to cardiac resynchronization therapy (CRT) remain controversial. We conducted a meta-analysis to summarize all published studies to determine whether sex-based differences in response to CRT exist.
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Cytokine-like Molecule CCDC134 Contributes to CD8+ T-cell Effector Functions in Cancer Immunotherapy.
Cancer Res.
PUBLISHED: 08-14-2014
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CCDC134 is a poorly characterized secreted protein that may act as an immune cytokine. Here, we show that CCDC134 is differentially expressed on resting and activated immune cells and that it promotes CD8(+) T-cell activation, proliferation, and cytotoxicity by augmenting expression of the T-cell effector molecules IFN?, TNF?, granzyme B, and perforin. CCDC134 facilitated infiltration of CD8(+) T cells with enhanced cytolytic activity into tumors, demonstrating strong antitumor effects in a CD8(+) T-cell-dependent manner. Mechanistically, in CD8(+) T cells, exposure to CCDC134 promoted cell proliferation through the JAK3-STAT5 pathway, a classic feature of many cytokines of the common ?-chain (?c) cytokine receptor family. Overall, our results provide evidence that CCDC134 may serve as a member of the ?c cytokine family and illustrate its potent antitumor effects by augmenting CD8(+) T-cell-mediated immunity. Cancer Res; 74(20); 5734-45. ©2014 AACR.
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The ratio of high-density lipoprotein cholesterol to apolipoprotein A-I predicts myocardial injury following elective percutaneous coronary intervention.
Clin Cardiol
PUBLISHED: 08-11-2014
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High-density lipoprotein (HDL) has cardioprotective properties. Each HDL particle has a few molecules of apolipoprotein A-I (apoA-I) and carries various amounts of cholesterol. The ratio of high-density lipoprotein cholesterol (HDL-C) to apoA-I may reflect mean HDL particle size.
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Lentivirus-mediated Wnt10b overexpression enhances fracture healing in a rat atrophic non-union model.
Biotechnol. Lett.
PUBLISHED: 07-16-2014
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Lentivirus vectors encoding Wnt10b gene (LV-Wnt10b) or luciferase gene (LV-luc) were constructed to determine whether Wnt10b overexpression improved fracture healing in a rat atrophic non-union model. After fracture, rats were injected with LV-Wnt10b or LV-luc. Luciferase signals were clearly detected. At 2 and 4 weeks, LV-Wnt10b group had 107 and 98 % more proliferating cell nuclear antigen (PCNA) positive cells, respectively, and promoted expression of bone morphogenetic protein-2 (BMP-2) in the callus compared with controls. LV-Wnt10b injection significantly increased bone mass density and bone mineral content: 46-84 and 96-193 %, respectively, at the site of fracturein the LV-Wnt10b group compared with controls. At 8 weeks, fractured femora were healed in the LV-Wnt10b group compared with atrophic non-unions formed in controls. Thus, Wnt10b overexpression associated with lentiviral gene therapy is effective in healing atrophic non-unions in rats.
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Cytotoxicity of the compounds isolated from Pulsatilla chinensis saponins and apoptosis induced by 23-hydroxybetulinic acid.
Pharm Biol
PUBLISHED: 07-16-2014
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Abstract Context: The rizoma of Pulsatilla chinensis (Bunge) Regel has been used as a traditional Chinese medicinal herb for thousands of years. Total saponins from P. chinensis can induce the apoptosis of solid cancer cells; however, their activity on chronic myeloid leukemia and the mechanisms remains unknown. Objective: To study the activity of total saponins and the main active fractions from P. chinensis saponins on chronic myeloid leukemia, and to illustrate the mechanisms underlying the anticancer activities. Materials and methods: The cytotoxic activity were assayed by MTT; cell cycle arrest and apoptosis were tested by flow cytometry system; changes in the mitochondrial membrane potential were determined using JC-1; and the apoptosis signaling pathway was determined by western blotting. Results: We demonstrated that total P. chinensis saponin displayed cytotoxic activity against K562 cell line. In addition, we identified 23-hydroxybetulinic acid (HBA), pulchinenoside A (PA), and anemoside B4 (AB4) from the total saponins, with the most cytotoxic compound HBA. Glycosylation at C3 and C28 of HBA significantly reduces its cytotoxicity. HBA could promote cell cycle arrest at S phase and induce apoptosis via intrinsic pathway. HBA disrupts mitochondrial membrane potential significantly (p?
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Percutaneous coronary intervention compared with coronary artery bypass graft in coronary artery disease patients with chronic kidney disease: a systematic review and meta-analysis.
Ren Fail
PUBLISHED: 07-02-2014
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Previous reports of percutaneous coronary intervention versus coronary artery bypass graft outcomes in coronary artery disease patients with chronic kidney disease (CKD) were inconsistent. We evaluated the optimal revascularization strategy for CKD patients. We searched Pub Med, EMBASE, and the Cochrane Central Register of Controlled Trials and scanned the references of relevant articles and reviews. All studies that compared relevant clinical outcomes between percutaneous coronary intervention and coronary artery bypass graft in CKD patients were selected. We defined short-term and long-term all-cause mortality as primary outcome, and long-term incidences of myocardial infarction and revascularization as secondary outcomes. A total of 2235 citations were retrieved, and 31 studies involving 99,054 patients, with 55,383 receiving percutaneous coronary intervention and 43,671 receiving coronary artery bypass graft, were included. In subgroup analyses of dialysis patients receiving percutaneous coronary intervention with stents versus coronary artery bypass graft, CKD patients with multivessel coronary disease, and CKD patients receiving drug-eluting stent versus coronary artery bypass graft, the pooled outcomes revealed that percutaneous coronary intervention possessed lower short-term mortality, but higher late revascularization risk. No significant differences in long-term mortality were observed between the two strategies in these subgroup analyses. In conclusion, in some specific clinical circumstances, CKD patients receiving percutaneous coronary intervention possessed lower short-term all-cause mortality, but higher long-term revascularization risk, than coronary artery bypass graft; long-term all-cause mortality was not different between the two strategies.
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Subclassification of left ventricular hypertrophy based on dilation stratifies coronary artery disease patients with distinct risk.
Eur. J. Clin. Invest.
PUBLISHED: 06-23-2014
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A new 4-tired classification of left ventricular hypertrophy (LVH) based on LV concentricity and dilation has been proposed; however, the association between the new categorization of LV geometry and outcomes in patients with coronary artery disease (CAD) is still unknown.
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[The effect of equilibrium psychological intervention in wounded patients in Lushan earthquake].
Sichuan Da Xue Xue Bao Yi Xue Ban
PUBLISHED: 06-20-2014
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To investigate the early psychological effect of the equilibrium psychological intervention on the person who were injured in the disaster incident.
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Biochar from Alternanthera philoxeroides could remove Pb(II) efficiently.
Bioresour. Technol.
PUBLISHED: 05-24-2014
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A novel bio-adsorbent was successfully synthesized by pyrolyzing Alternanthera philoxeroides (AP), one of the most widely used hydrophytes for eutrophic lake ecological restoration under O2-limited condition at 600 °C. Compared with commercially active carbon (AC), the initial solution pH had a weak effect on the adsorption of Pb(II) by AP biochar (APB). The maximum adsorption capacity of APB for Pb(II) was 257.12 mg/g, which was 5.3 times of that of the AC. The adsorption process was fast, with only 2.5h to reach adsorption equilibrium. The adsorption mechanism of Pb(II) by APB involves the precipitation and complexation of Pb(II) with free carboxyl/hydroxyl functional groups and mineral carbonates of APB as well as ion replacement between Pb(II) and alkaline earth cations. These results suggest that using a low-cost APB adsorbent for heavy metals contaminated water treatment may have great ecological and environmental significance.
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Poor ability to resist tempting calorie rich food is linked to altered balance between neural systems involved in urge and self-control.
Nutr J
PUBLISHED: 05-01-2014
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The loss of self-control or inability to resist tempting/rewarding foods, and the development of less healthful eating habits may be explained by three key neural systems: (1) a hyper-functioning striatum system driven by external rewarding cues; (2) a hypo-functioning decision-making and impulse control system; and (3) an altered insula system involved in the translation of homeostatic and interoceptive signals into self-awareness and what may be subjectively experienced as a feeling.
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An alternative feedstock of corn meal for industrial fuel ethanol production: delignified corncob residue.
Bioresour. Technol.
PUBLISHED: 04-25-2014
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Delignified corncob residue is an industrial solid waste from xylose production using corncob as feedstock. In this study, delignified corncob residue was used as the feedstock of ethanol production by simultaneous saccharification and fermentation (SSF) and the optimal fermentation performance was investigated under various operation conditions. The ethanol titer and yield reached 75.07 g/L and 89.38%, respectively, using a regular industrial yeast strain at moderate cellulase dosage and high solids loading. A uniform SSF temperature of 37°C at both prehydrolysis and SSF stages was tested. The fermentation performance and cost of delignified corncob residue and corn meal was compared as feedstock of ethanol fermentation. The result shows that the delignified corncob residue is competitive to corn meal as ethanol production feedstock. The study gives a typical case to demonstrate the potential of intensively processed lignocellulose as the alternative feedstock of corn meal for industrial fuel ethanol production.
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Association of preprocedural low-density lipoprotein cholesterol levels with myocardial injury after elective percutaneous coronary intervention.
J Clin Lipidol
PUBLISHED: 04-15-2014
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Lower levels of low-density lipoprotein cholesterol (LDL-C) are associated with less cardiovascular risk in patients with coronary artery disease.
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[Feasibility study on Xelox (capecitabine plus oxapliplatin) in the volumetric modulated arc therapy (VMAT)-based preoperative chemoradiotherapy for locally advanced rectal cancer].
Zhonghua Wei Chang Wai Ke Za Zhi
PUBLISHED: 03-28-2014
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To investigate the feasibility of Xelox(capecitabine plus oxaliplatin) in the volumetric modulated arc therapy(VMAT)-based preoperative chemoradiotherapy (CRT) for locally advanced rectal cancer(LARC).
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Effects of Nardostachys chinensis on spontaneous ventricular arrhythmias in rats with acute myocardial infarction.
J. Cardiovasc. Pharmacol.
PUBLISHED: 03-26-2014
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To investigate the effects and mechanisms of Nardostachys chinensis (NC) on spontaneous ventricular arrhythmias in rats with hyper-acute myocardial infarction (AMI).
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Elevated serum level of IL-27 and VEGF in patients with ankylosing spondylitis and associate with disease activity.
Clin. Exp. Med.
PUBLISHED: 03-25-2014
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Interleukin (IL)-27 is an IL-12 family cytokine and exerts a critical role in immune regulation in the context of infection, autoimmunity, and angiogenesis. In this study, we aimed to investigate the possible pathophysiological role of IL-27 and vascular endothelial growth factor (VEGF) in ankylosing spondylitis (AS). One hundred and forty AS patients and 90 healthy controls were included in the current study. The levels of IL-27 and VEGF in serum and synovial fluid (SF) samples were measured by enzyme-linked immunosorbent assay. Erythrocyte sedimentation rate, C-reactive protein, and human leukocyte antigen (HLA)-B27 were measured by standard laboratory techniques. Disease activity in AS was scored with Bath Ankylosing Spondylitis Disease Activity Index (BASDAI). Hip involvement, peripheral arthritis, and eye involvement were also recorded. The serum levels of IL-27 were remarkably higher in AS patients than healthy groups and significantly correlated with serum levels of VEGF. Furthermore, the serum levels of IL-27 were correlated with BASDAI independent of other markers of inflammation. Elevated serum levels of IL-27 and VEGF were detected in AS patients with peripheral arthritis and HLA-B27 positive. The SF levels of IL-27 and VEGF were significantly higher than serum levels in AS patients with peripheral arthritis. By contrast, levels of IL-27 and VEGF were not increased in AS patients with hip involvement and eye involvement. IL-27 may regulate the immunological or inflammatory process of AS.
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Helicobacter pylori infection, gastrin and cyclooxygenase-2 in gastric carcinogenesis.
World J. Gastroenterol.
PUBLISHED: 03-12-2014
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Gastric cancer is one of the most frequent neoplasms and a main cause of death worldwide, especially in China and Japan. Numerous epidemiological, animal and experimental studies support a positive association between chronic Helicobacter pylori (H. pylori) infection and the development of gastric cancer. However, the exact mechanism whereby H. pylori causes gastric carcinogenesis remains unclear. It has been demonstrated that expression of cyclooxygenase-2 (COX-2) is elevated in gastric carcinomas and in their precursor lesions. In this review, we present the latest clinical and experimental evidence showing the role of gastrin and COX-2 in H. pylori-infected patients and their possible association with gastric cancer risk.
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Characterization of the anticancer effects of S115, a novel heteroaromatic thiosemicarbazone compound, in vitro and in vivo.
Acta Pharmacol. Sin.
PUBLISHED: 03-06-2014
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To investigate the anticancer effects of S115, a novel heteroaromatic thiosemicarbazone compound in vitro and in vivo.
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Significance of red cell distribution width measurement for the patients with isolated coronary artery ectasia.
J Transl Med
PUBLISHED: 03-03-2014
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Red cell distribution width (RDW) has been recognized as a novel marker for several cardiovascular diseases. The aim of this study was to evaluate the association between RDW levels and the presence of isolated coronary artery ectasia (CAE).
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Xiangshaliujunzi Decoction for the treatment of diabetic gastroparesis: a systematic review.
World J. Gastroenterol.
PUBLISHED: 02-28-2014
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To assess the current clinical evidence of the effectiveness of Xiangshaliujunzi Decoction (XSLJZD) for the treatment of diabetic gastroparesis (DGP).
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Cervical cancer screening and analysis of potential risk factors in 43,567 women in Zhongshan, China.
Asian Pac. J. Cancer Prev.
PUBLISHED: 02-27-2014
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The objective of this study was to establish a program model for use in wide-spread cervical cancer screening.
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Combination of symptoms, syndrome and disease: treatment of refractory diabetic gastroparesis.
World J. Gastroenterol.
PUBLISHED: 02-24-2014
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To assess effect of combination of symptoms, syndrome and disease on treatment of diabetic gastroparesis with severe nausea and vomiting.
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Design of a prognostic score model for nasopharyngeal carcinoma.
Head Neck
PUBLISHED: 02-21-2014
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The purpose of this analysis was to create a prognostic score model for newly diagnosed nasopharyngeal carcinoma (NPC) which has not been created before.
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Decreased expression of elastin and lysyl oxidase family genes in urogenital tissues of aging mice.
J. Obstet. Gynaecol. Res.
PUBLISHED: 02-17-2014
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This study aims to investigate the expression levels of elastin and lysyl oxidase (LOX) family members in the urogenital tissues of natural aging mice and accelerated ovarian aging mice.
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Glycosylated hemoglobin A1c as a marker predicting the severity of coronary artery disease and early outcome in patients with stable angina.
Lipids Health Dis
PUBLISHED: 02-16-2014
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Glycosylated hemoglobin A1C (HbA1c) has been widely recognized as a marker for predicting the severity of diabetes mellitus (DM) and several cardiovascular diseases. However, whether HbA1c could predict the severity and clinical outcomes in patients with stable coronary artery disease (CAD) remains largely unknown. We determine relationship of HbA1c with severity and outcome in patients with stable CAD.
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Spherical blackberry-type capsules containing block copolymer aggregates.
Langmuir
PUBLISHED: 02-14-2014
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The design, preparation, and properties of nanosized blackberry-like structures are described. These capsules are composed of two layers of individual block copolymer aggregates, relatively large core vesicles onto which is deposited a layer of smaller vesicles or micelles. The composition of the adjacent layers is such as to ensure strong electrostatic interactions between them. The core vesicles are typically composed of either PS-b-P4VP with a positively charged corona or of PS-b-PAA with a negatively charged corona, and are surrounded by a layer of smaller, oppositely charged block copolymer vesicles or micelles. These composite structures bear a strong resemblance to blackberries, hence the proposed name. The blackberry structures can be prepared in solution or on a flat surface, for example, a silicon wafer. Four compositional possibilities for the blackberries structures were studied, in which the positively or negatively charged core vesicles are covered either by a layer of oppositely charged micelles or by vesicles. These structures represent the earliest stage of a layer-by-layer approach of small spherical aggregates onto a larger spherical hollow core. The strong interaction between the contacting layers is achieved by electrostatic interactions or by complementary acid-base properties, for example, H-bonding. These multicompartmented capsules could be used potentially as delivery vehicles for multiple components; each layer of the capsules could be loaded with hydrophobic (in the core of the micelles or vesicle wall) or hydrophilic molecules (in the vesicle cavity). The overall size of such structures can vary, but in any case can be kept below 1 ?m.
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[Advances in new chemotherapeutic drugs for preoperative chemoradiation of locally advanced rectal cancer].
Zhonghua Wei Chang Wai Ke Za Zhi
PUBLISHED: 02-13-2014
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Preoperative concurrent chemoradiotherapy based on 5-fluorouracil(5-FU) is an standard treatment mode for patients with locally advanced rectal cancer(LARC). Currently, more and more interests has now focused on new chemotherapeutic drugs, such as capecitabine, oxaliplatin, irinotecan, bevacizumab, and cetuximab in this treatment mode. Many prospective phase I(-III( clinical trials have been developed to explore these new drugs efficacy in the neoadjuvant chemoradiation(nCRT) for patients with LARC. Some results are very encouraging, yet others are undesirable. Capecitabine has been widely recognized in the nCRT for patients with LARC, and has the tendency to replace 5-FU. However, there are some controversies for oxaliplatin, irinotecan, and biologically targeted drugs in the nCRT mode because of their limited clinical benefits. It is potentially the development direction to study the mutual interaction mechanism among concurrent drugs or radiation and biologically targeted drugs, find new predicatively responsive targets, and screen the appropriate patient in the treatment of neoCRT for patients with LARC in the future.
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Impact of admission triglyceride for early outcome in diabetic patients with stable coronary artery disease.
Lipids Health Dis
PUBLISHED: 02-12-2014
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The role of triglyceride (TG) in predicting the outcomes in diabetic patients with coronary artery disease (CAD) has not been well investigated.
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Were you identified to be at high fracture risk by FRAX® before your osteoporotic fracture occurred?
Clin. Rheumatol.
PUBLISHED: 02-09-2014
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With the increasing longevity and the growing size of the aging population, China is among the developing countries with the greatest burden of osteoporotic fractures (OFs). Identification of individuals at high fracture risk to provide early treatment in developing countries with limited resources remains a challenge. Our aim was to calculate the FRAX® values in a selected mainland Chinese population the day before the occurrence of an OF and to compare the results with the Osteoporosis Foundation (NOF) thresholds. To define a population at high fracture risk, we selected patients in the orthopedic emergency cohort from Shanghai Sixth People's Hospital, China. To calculate the FRAX® the day before the actual OF, the actual fracture was not considered as a previous fracture. Subjects with a FRAX® value lower than the NOF thresholds were not considered at high risk. One-hundred ninety-eight patients were included with a mean age of 69.9?±?10.1 years old, and 78.8 % were female. Major fracture sites included hip (41.9 %), proximal humerus (22.2 %), forearm (27.8 %), spine (6.6 %), and pelvis (1.5 %). Overall, only two (1.0 %) patients had a 10-year major fracture probability ?20 %, and 84 (42.4 %) patients had a 10-year hip fracture probability ?3 %. More than half of the population was not identified to be at high risk by the FRAX® BMI (body mass index). For age groups 55-59, 60-69, 70-79, and 80-90 years old, respectively, the mean FRAX® BMI values (major OF/hip fracture) were 3.5/0.7, 5.7/1.8, 8.2/4.7, and 8.6/4.8 %. Dual-energy X-ray absorptiometry was available for 58 patients, and 41.2 % had a T-score???-2.5 standard deviation (lumbar spine and/or femoral neck and/or total hip). Only six (17.6 %) patients with a normal or osteopenic BMD were classified at high risk by the FRAX® BMD (bone mineral density). The NOF thresholds could only identify fewer than half of the studied population to be at high risk the day before an actual OF, and the 10-year hip fracture probability seemed to perform better than the 10-year major fracture probability. Prospective population-based studies are needed to validate the prediction of Chinese FRAX® model and to establish ideal thresholds.
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Clinical features of coronary artery ectasia in the elderly.
J Geriatr Cardiol
PUBLISHED: 02-08-2014
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To investigate the incidence, imaging and clinical characteristics in elderly patients with coronary artery ectasia (CAE).
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Angiotensin-converting enzyme I/D polymorphism is associated with pneumonia risk: A meta-analysis.
J Renin Angiotensin Aldosterone Syst
PUBLISHED: 02-06-2014
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Previous studies examined the association between angiotensin-converting enzyme (ACE) I/D polymorphism and pneumonia, but their results were inconsistent. Thus, a meta-analysis was performed to clarify the effect of ACE I/D polymorphism on pneumonia risk and pneumonia-related mortality.
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Clinical efficacy of aconitum-containing traditional Chinese medicine for diabetic peripheral neuropathic pain.
Am. J. Chin. Med.
PUBLISHED: 01-29-2014
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Diabetic peripheral neuropathy is a common chronic complication of diabetes. Routine clinical management uses analgesics to relieve pain in combination with drugs for nerve repair. The drugs are often not effective for the severe pain cases, and these western medications also have side effects. We report a more effective treatment of diabetic peripheral neuropathic pain using a high dose of a traditional Chinese medicine, aconitum (including both Radix aconite preparata and Radix aconite kusnezoffii), in combination with Huangqi Guizhi Wuwu Tang (i.e., astragalus, cassia twig, white peony root, and spatholobi). In order to achieve stronger analgesic effects, we increased the clinical dosage of aconitum from 15 to 120 g. The aconitum was boiled for 6-8 hours, and licorice was also used to reduce potential toxicities of aconitum. In the four reported cases, the patients' neuropathic pain was remarkably reduced and the EMG profile was also improved with this treatment regimen. Adverse reactions were not observed during the therapy. Thus, aconitum represents a promising and safe treatment for the well-being of patients and their diabetic peripheral neuropathic pain. Future controlled clinical trials using traditional Chinese medicines containing aconitum in treating the neuropathic pain are warranted.
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Impact of short-term low-dose atorvastatin on low-density lipoprotein and high-density lipoprotein subfraction phenotype.
Clin. Exp. Pharmacol. Physiol.
PUBLISHED: 01-28-2014
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Statins can significantly reduce low-density lipoprotein-cholesterol (LDL-C) and modestly raise or not alter high-density lipoprotein-cholesterol (HDL-C). However, their impact on high-density lipoprotein (HDL) and low-density lipoprotein (LDL) subfractions has been less examined. The aim of the present study was to investigate the short-term impact of low-dose atorvastatin on HDL and LDL subfractions in humans. In this randomized study, data from 52 subjects were analysed. Thirty-seven patients with atherosclerosis were randomized to treatment with atorvastatin 10 mg/day (n = 17) or 20 mg/day (n = 20) for 8 weeks, with 15 healthy subjects without therapy used as a control group. The lipid profile and lipoprotein subfractions were determined using the Lipoprint system at baseline and at 8 weeks. The data suggest that atorvastatin treatment (10 and 20 mg/day) for 8 weeks significantly decreases LDL-C levels and reduces the cholesterol concentration of all LDL subfractions, which is accompanied by an increase of the mean LDL particle size. Although 10 mg/day atorvastatin treatment for 8 weeks had no impact on the HDL subfraction, 20 mg/day atorvastatin for 8 weeks significantly increased the cholesterol concentration of large HDL particles and decreased the cholesterol concentration of small HDL particles without changing serum HDL-C levels in patients with atherosclerosis. Therefore, the results suggest that 20 mg/day atorvastatin treatment for 8 weeks may result in a favourable modification of the HDL subfraction phenotype in addition to its effects on the cholesterol concentration of all LDL subfractions and mean LDL particle size.
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Association of fibrinogen with severity of stable coronary artery disease in patients with type 2 diabetic mellitus.
Dis. Markers
PUBLISHED: 01-26-2014
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Some studies have suggested a relation of plasma fibrinogen to the severity of coronary artery disease (CAD). However, whether plasma fibrinogen can predict the presence and severity of CAD in patients with diabetes mellitus has not been determined.
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The combination of atomic force microscopy and sugar analysis to evaluate alkali-soluble Canna edulis Ker pectin.
Food Chem
PUBLISHED: 01-26-2014
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Alkali-soluble pectin, which has been extracted from Canna edulis Ker, was characterized by single sugar determination and atomic force microscopy (AFM). The results indicated that the amounts of four predominant sugars including arabinose (Ara), glucose (Glc), galactose (Gal) and galacturonic acid (GalA) significantly decreased during the process of mild acid hydrolysis. The decreasing rates of these four sugars followed a sequential order of Ara>Gal>Glc>GalA. The homogalacturonan (HG) chain present in pectin, and the quantity of branched material is greater than the sample containing the main neutral sugars. The results indicated that the neutral sugar and HG side chains are attached to pectin as part of the rhamnogalacturonan I (RGI) complex. Moreover, hydrolysis leads to the reduction of mean lengths of backbone and branch, as well as the number/weight-average molecular weight. Meanwhile, the amount of short chain fractions increased during hydrolysis. Furthermore, the decrease of the polymerization degree of alkali-soluble C. edulis pectin as a function of the hydrolysis time could be described by a first-order exponential decay function.
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Bis(2,3-dibromo-4,5-dihydroxybenzyl) ether, a marine algae derived bromophenol, inhibits the growth of Botrytis cinerea and interacts with DNA molecules.
Mar Drugs
PUBLISHED: 01-23-2014
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Bis(2,3-dibromo-4,5-dihydroxybenzyl) ether (BDDE) is a bromophenol isolated from marine algae. Previous reports have shown that BDDE possesses cytotoxic and antibacterial activity. In the present study, we demonstrate that BDDE displays broad-spectrum antifungal activities, especially on Botrytis cinerea. BDDE inhibits the growth of B. cinerea cultured on a solid medium of potato dextrose agar (PDA) as well as on the potato dextrose broth (PDB) medium. Moreover, BDDE decreases the incidence of fruit decay and severity of strawberries infected with B. cinerea. Further studies have revealed that BDDE decreases the germination rate and inhibits the mycelial growth of B. cinerea. The inhibition mechanisms are related to the disruption of the cell membrane integrity in B. cinerea spores and newly formed germ tubes. This study also suggests that BDDE possibly interacts with DNA via intercalation and minor groove binding. The studies provide evidence that BDDE has potential application in the control of gray mold after fruit harvest and the compound could serve as a candidate or lead template for rational drug design and for the development of antifungal agents.
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NEDD9 overexpression is associated with the progression of and an unfavorable prognosis in epithelial ovarian cancer.
Hum. Pathol.
PUBLISHED: 01-21-2014
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Neural precursor cell-expressed, developmentally down-regulated 9 (NEDD9), a scaffolding protein, has been identified as a prometastatic and poor prognostic gene in multiple malignant tumors. However, the potential role of the NEDD9 protein in epithelial ovarian cancer (EOC) remains unclear. In the present study, we investigated the expression of NEDD9 and the correlation between NEDD9 expression and prognosis in EOC. NEDD9 expression was detected in 129 archived EOC specimens by immunohistochemical staining and in 28 freshly frozen EOC specimens by Western blotting. The expression of NEDD9 was evaluated in ovarian cancer cell lines by Western blotting and immunofluorescence. The association between the expression of NEDD9 and prognosis was determined by survival analysis. Results suggested that NEDD9 was overexpressed in EOC specimens compared with noninvasive epithelial ovarian tumors and normal ovarian specimens. A high level of NEDD9 expression significantly correlated with advanced-stage tumors (International Federation of Gynecology and Obstetrics classes III-IV, P < .001), high-grade carcinoma (grades 2-3, P < .001), and suboptimal primary cytoreductive surgery (residual disease <1cm, P = .021). The expression level of NEDD9 varied in ovarian cancer cell lines. Multivariate analysis indicated that NEDD9 overexpression (P = .033), advanced stage (P < .001), and high-grade carcinoma (P = .01) were independent predictors of poor survival. In conclusion, NEDD9 is overexpressed and associated with an unfavorable prognosis in EOC. NEDD9 overexpression is an independent factor of poor prognosis and may serve as a potential biomarker in EOC.
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Treatment of refractory diabetic gastroparesis: Western medicine and traditional Chinese medicine therapies.
World J. Gastroenterol.
PUBLISHED: 01-13-2014
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Refractory diabetic gastroparesis (DGP), a disorder that occurs in both type 1 and type 2 diabetics, is associated with severe symptoms, such as nausea and vomiting, and results in an economic burden on the health care system. In this article, the basic characteristics of refractory DGP are reviewed, followed by a discussion of therapeutic modalities, which encompasses the definitions and clinical manifestations, pathogenesis, diagnosis, and therapeutic efficacy evaluation of refractory DGP. The diagnostic standards assumed in this study are those set forth in the published literature due to the absence of recognized diagnosis criteria that have been assessed by an international organization. The therapeutic modalities for refractory DGP are as follows: drug therapy, nutritional support, gastric electrical stimulation, pyloric botulinum toxin injection, endoscopic or surgical therapy, and traditional Chinese treatment. The therapeutic modalities may be used alone or in combination. The use of traditional Chinese treatments is prevalent in China. The effectiveness of these therapies appears to be supported by preliminary evidence and clinical experience, although the mechanisms that underlie these effects will require further research. The purpose of this article is to explore the potential of combined Western and traditional Chinese medicine treatment methods for improved patient outcomes in refractory DGP.
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Planar multilayer assemblies containing block copolymer aggregates.
Langmuir
PUBLISHED: 01-13-2014
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The design, preparation, and properties of planar multilayer structures composed of various combinations of sequentially deposited polyelectrolyte (PE) chains and self-assembled layers of individual block copolymer aggregates (vesicles, micelles, or large compound micelles (LCMs)) are described. The aggregates contain negatively or positively charged corona chains while the PE multilayers contain alternating polyanionic or polycationic chains deposited on silicon wafers. The final structures consist of combinations of layers of various charged species: multilayers of alternating PEs of poly(allyl hydrochloride) (PAH) and poly(acrylic acid) (PAA) as well as vesicles, micelles, or large compound micelles of ionized poly(styrene)-b-poly(4-vinylpyridine) (PS-b-P4VP) or of poly(styrene)-b-poly(acrylic acid) (PS-b-PAA). Two types of layer-by-layer (LbL) multilayer structures were studied: individual aggregate layers sandwiched between PE multilayers and layers of individual aggregates of various morphologies and of different corona chain charges, deposited on top of each other without intermediate multilayers or individual layers of PEs. The strong interactions between the successive layers are achieved mainly by electrostatic attraction between the oppositely charged layers. The planar LbL multilayers containing block copolymer aggregates could, potentially, be used as carriers for multiple functional components; each aggregate layer could be loaded with hydrophobic (in the core of the micelles, LCMs, or vesicle walls) or hydrophilic functional molecules (in the vesicular cavities). The overall thickness of such planar LbL multilayers can be controlled precisely and can vary from tens of nanometers to several micrometers depending on the number of layers, the sizes of the aggregates, and the complexity of the structure.
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CS5931, a novel polypeptide in Ciona savignyi, represses angiogenesis via inhibiting vascular endothelial growth factor (VEGF) and matrix metalloproteinases (MMPs).
Mar Drugs
PUBLISHED: 01-09-2014
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CS5931 is a novel polypeptide from Ciona savignyi with anticancer activities. Previous study in our laboratory has shown that CS5931 can induce cell death via mitochondrial apoptotic pathway. In the present study, we found that the polypeptide could inhibit angiogenesis both in vitro and in vivo. CS5931 inhibited the proliferation, migration and formation of capillary-like structures of HUVECs (Human Umbilical Vein Endothelial Cell) in a dose-dependent manner. Additionally, CS5931 repressed spontaneous angiogenesis of the zebrafish vessels. Further studies showed that CS5931 also blocked vascular endothelial growth factor (VEGF) production but without any effect on its mRNA expression. Moreover, CS5931 reduced the expression of matrix metalloproteinases (MMP-2 and MMP-9) both on protein and mRNA levels in HUVEC cells. We demonstrated that CS5931 possessed strong anti-angiogenic activity both in vitro and in vivo, possible via VEGF and MMPs. This study indicates that CS5931 has the potential to be developed as a novel therapeutic agent as an inhibitor of angiogenesis for the treatment of cancer.
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Limited Density of an Antigen Presented by RMA-S Cells Requires B7-1/CD28 Signaling to Enhance T-Cell Immunity at the Effector Phase.
PLoS ONE
PUBLISHED: 01-01-2014
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The association of B7-1/CD28 between antigen presenting cells (APCs) and T-cells provides a second signal to proliferate and activate T-cell immunity at the induction phase. Many reports indicate that tumor cells transfected with B7-1 induced augmented antitumor immunity at the induction phase by mimicking APC function; however, the function of B7-1 on antitumor immunity at the effector phase is unknown. Here, we report direct evidence of enhanced T-cell antitumor immunity at the effector phase by the B7-1 molecule. Our experiments in vivo and in vitro indicated that reactivity of antigen-specific monoclonal and polyclonal T-cell effectors against a Lass5 epitope presented by RMA-S cells is increased when the cells expressed B7-1. Use of either anti-B7-1 or anti-CD28 antibodies to block the B7-1/CD28 association reduced reactivity of the T effectors against B7-1 positive RMA-S cells. Transfection of Lass5 cDNA into or pulse of Lass5 peptide onto B7-1 positive RMA-S cells overcomes the requirement of the B7-1/CD28 signal for T effector response. To our knowledge, the data offers, for the first time, strong evidence that supports the requirement of B7-1/CD28 secondary signal at the effector phase of antitumor T-cell immunity being dependent on the density of an antigenic peptide.
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Relationship of glycated hemoglobin levels with myocardial injury following elective percutaneous coronary intervention in patients with type 2 diabetes mellitus.
PLoS ONE
PUBLISHED: 01-01-2014
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Glycated hemoglobin (HbA1c) predicts clinical cardiovascular disease or cardiovascular mortality. However, the relationship between HbA1c and myocardial injury following elective percutaneous coronary intervention (PCI) in patients with type 2 diabetes mellitus (DM) has not been investigated.
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Relation of leukocytes and its subsets counts with the severity of stable coronary artery disease in patients with diabetic mellitus.
PLoS ONE
PUBLISHED: 01-01-2014
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Both coronary artery disease (CAD) and diabetes mellitus (DM) are associated with inflammation. However, whether and which leukocytes can predict the presence and extent of CAD in patients with DM has not been investigated. The aim of the present study was to examine the association of leukocyte and its subsets counts with the severity of CAD in patients with DM.
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Zinc ions upregulate the hormone gastrin via an E-box motif in the proximal gastrin promoter.
J. Mol. Endocrinol.
PUBLISHED: 12-24-2013
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Gastrin and its precursors act as growth factors for the normal and neoplastic gastrointestinal mucosa. As the hypoxia mimetic cobalt chloride upregulates the gastrin gene, the effect of other metal ions on gastrin promoter activity was investigated. Gastrin mRNA was measured by real-time PCR, gastrin peptides by RIA, and gastrin promoter activity by dual-luciferase reporter assay. Exposure to Zn(2)(+) ions increased gastrin mRNA concentrations in the human gastric adenocarcinoma cell line AGS in a dose-dependent manner, with a maximum stimulation of 55±14-fold at 100??M (P<0.05). Significant stimulation was also observed with Cd(2)(+) and Cu(2)(+), but not with Ca(2)(+), Mg(2)(+), Ni(2)(+), or Fe(3)(+) ions. Activation of MAPK and phosphatidylinositol 3-kinase pathways is necessary but not sufficient for gastrin induction by Zn(2)(+). Deletional mutation of the gastrin promoter identified an 11?bp DNA sequence, which contained an E-box motif, as necessary for Zn(2)(+)-dependent gastrin induction. The fact that E-box binding transcription factors play a crucial role in the epithelial-mesenchymal transition (EMT), together with our observation that Zn(2)(+) ions upregulate the gastrin gene in AGS cells by an E-box-dependent mechanism, suggests that Zn(2)(+) ions may induce an EMT, and that gastrin may be involved in the transition.
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[Value of postoperative adjuvant chemotherapy in locally advanced rectal cancer patients with ypT1-4N0 after neo-adjuvant chemoradiotherapy].
Zhonghua Zhong Liu Za Zhi
PUBLISHED: 12-17-2013
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The purpose of this study was to investigate the value of postoperative chemotherapy for locally advanced rectal cancer patients who reached pathological ypT1-4N0 after neo-adjuvant chemoradiotherapy.
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[Functional polymorphism of NRF2 gene promoter -617C/A in lipopolysaccharide-stimulated peripheral blood mononuclear cellular inflammatory response in patients with alcoholic liver disease].
Zhonghua Nei Ke Za Zhi
PUBLISHED: 11-26-2013
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To investigate the influence of NRF2 gene polymorphism at locus -617 on inflammatory response of lipopolysaccharide (LPS)-stimulated peripheral blood mononuclear cells (PBMCs) in patients with alcoholic liver disease (ALD).
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Occurrence and Distribution of Antibiotic-resistant Bacteria and Transfer of Resistance Genes in Lake Taihu.
Microbes Environ.
PUBLISHED: 11-16-2013
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The overuse of antibiotics has accelerated antibiotic resistance in the natural environment, especially fresh water, generating a potential risk for public health around the world. In this study, antibiotic resistance in Lake Taihu was investigated and this was the first thorough data obtained through culture-dependent methods. High percentages of resistance to streptomycin and ampicillin among bacterial isolates were detected, followed by tetracycline and chloramphenicol. Especially high levels of ampicillin resistance in the western and northern regions were illustrated. Bacterial identification of the isolates selected for further study indicated the prevalence of some opportunistic pathogens and 62.0% of the 78 isolates exhibited multiple antibiotic resistance. The presence of ESBLs genes was in the following sequence: blaTEM > blaSHV > blaCTMX and 38.5% of the isolates had a class I integrase gene. Of all tested strains, 80.8% were able to transfer antibiotic resistance through conjugation. We also concluded that some new families of human-associated ESBLs and AmpC genes can be found in natural environmental isolates. The prevalence of antibiotic resistance and the dissemination of transferable antibiotic resistance in bacterial isolates (especially in opportunistic pathogens) was alarming and clearly indicated the urgency of realizing the health risks of antibiotic resistance to human and animal populations who are dependent on Lake Taihu for water consumption.
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Association of microRNA-126 expression with clinicopathological features and the risk of biochemical recurrence in prostate cancer patients undergoing radical prostatectomy.
Diagn Pathol
PUBLISHED: 11-14-2013
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Numerous studies have suggested that microRNA-126 (miR-126) is involved in development of various cancer types as well as in malignant proliferation and invasion. However, its role in human prostate cancer (PCa) is still unclear. The aim of this study was to investigate miR-126 expression in PCa and its prognostic value for PCa patients undergoing radical prostatectomy.
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The substantial hospitalization burden of influenza in central China: surveillance for severe, acute respiratory infection, and influenza viruses, 2010-2012.
Influenza Other Respir Viruses
PUBLISHED: 09-26-2013
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Published data on influenza in severe acute respiratory infection (SARI) patients are limited. We conducted SARI surveillance in central China and estimated hospitalization rates of SARI attributable to influenza by viral type/subtype.
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Sex-related differences in outcomes after transcatheter aortic valve implantation: a systematic review and meta-analysis.
Circ Cardiovasc Interv
PUBLISHED: 09-24-2013
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There were considerable discrepancies with regard to sex-related differences in complications and prognosis after transcatheter aortic valve implantation.
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Identification of a stable molecular signature in mammary tumor endothelial cells that persists in vitro.
Angiogenesis
PUBLISHED: 09-11-2013
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Long-term, in vitro propagation of tumor-specific endothelial cells (TEC) allows for functional studies and genome-wide expression profiling of clonally derived, well-characterized subpopulations. Using a genetically engineered mouse model of mammary adenocarcinoma, we have optimized an isolation procedure and defined growth conditions for long-term propagation of mammary TEC. The isolated TEC maintain their endothelial specification and phenotype in culture. Furthermore, gene expression profiling of multiple TEC subpopulations revealed striking, persistent overexpression of several candidate genes including Irx2 and Zfp503 (transcription factors), Alcam and Cd133 (cell surface markers), Ccl4 and neurotensin (Nts) (angiocrine factors), and Gpr182 and Cnr2 (G protein-coupled receptors). Taken together, we have developed an effective method for isolating and culture-expanding mammary TEC, and uncovered several new TEC-selective genes whose overexpression persists even after long-term in vitro culture. These results suggest that the tumor microenvironment may induce changes in vascular endothelium in vivo that are stably transmittable in vitro.
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Physicochemical property of starch-pectin conjugates with its resistance to enzymatic activity.
J. Sci. Food Agric.
PUBLISHED: 08-15-2013
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In view of high nutritional values and bioactivities of resistant starch and pectin, it will be a good choice to chemically combine pectin into starch to give a new type of chemically modified resistant starch.
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Plasma homocysteine concentrations in the acute phase after central retinal vein occlusion in a Chinese population.
Curr. Eye Res.
PUBLISHED: 07-25-2013
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To explore the relationship between total plasma homocysteine (tHcy) concentration and central retinal vein occlusion (CRVO) in the acute phase in a Chinese population.
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[Analysis on dosage of traditional Chinese medicine decoction pieces stipulated in Chinese pharmacopoeia].
Zhongguo Zhong Yao Za Zhi
PUBLISHED: 07-16-2013
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Chinese Pharmacopoeia I (2010 edition) covers dosage and usage of traditional Chinese medicinal herbs and decoction pieces, and provides dosage ranges of most of decoction pieces. By using the descriptive statistical method, the article discusses the distribution of maximum dosage, minimum dosage and dosage range of decoction pieces set forth in Chinese Pharmacopoeia, and compares toxic drugs and non-toxic drugs. Altogether 617 drugs are included into the study. Except for 16 decoction pieces whose dosages are not clear, all of the remaining decoction pieces are covered by Chinese Pharmacopoeia, with the maximum common dosage, minimum common dosage and dosage range of 3, 10 and 6 g. Upon comparison, we discovered that Chinese Pharmacopoeia sets stricter standards for toxic drugs than non-toxic drugs. Compared with dosages in classical prescriptions and actual clinical usages, dosage ranges described in Chinese Pharmacopoeia are much narrower. There is no significant difference between drugs that can be used as foods or healthcare foods and other drugs according to Chinese Pharmacopoeia.
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Functional imaging of an alcohol-Implicit Association Test (IAT).
Addict Biol
PUBLISHED: 07-04-2013
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This research assessed activation in neural substrates involved in implicit associative processes through functional magnetic resonance imaging of an alcohol-Implicit Association Test (IAT) focused on positive outcomes of alcohol use. Comparisons involved 17 heavy and 19 light drinkers, ranging in age from 18 to 22, during compatible and incompatible association task trials. Behaviorally, a significant IAT effect was found with heavy drinkers showing stronger positive implicit associations toward alcohol use than light drinkers. Imaging data revealed heavy drinkers showed greater activity during compatible trials relative to incompatible trials in the left putamen and insula while no significant difference in activity between conditions was found in the light drinkers. Light drinkers showed significantly more activity in the left orbital frontal cortex during both compatible and incompatible trials than heavy drinkers, and the dorsolateral prefrontal cortex was engaged more in both light and heavy drinkers during incompatible trials relative to compatible trials. Further, within-group analyses showed significant amygdala activity along with the putamen and insula among heavy drinkers during compatible trials relative to incompatible trials. These results are consistent with a dual process framework of appetitive behaviors proposing that (1) implicit associations underlying habit are mediated through neural circuitry dependent on the striatum, and (2) controlled behaviors are mediated through neural circuitry more dependent on the prefrontal cortex. This is the first study to evaluate the neural mechanisms elicited by an alcohol-IAT, providing an additional step toward increasing understanding of associative habit processes and their regulatory influence over addictive behaviors.
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Distribution of nutrients, heavy metals, and PAHs affected by sediment dredging in the Wujingang River basin flowing into Meiliang Bay of Lake Taihu.
Environ Sci Pollut Res Int
PUBLISHED: 06-13-2013
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This papers survey of the pollution of the Wujingang River is important because it is one of the main rivers flowing into Meiliang Bay of Lake Taihu in eastern China. Trace metals (TMs) in this paper are described according to their pollution index (P i). Cluster analysis and correlation analysis are utilized for group sites and to assess co-contamination. Toxicity effect analysis was conducted using individual sediment quality guideline quotients (SQGQs) and mean SQGQs. The results showed that sediment from the Wujingang River basin was affected by nutrients, heavy metals, and polycyclic aromatic hydrocarbons (PAHs), which are an essential contamination source for both Meiliang Bay and Zhushan Bay of Lake Taihu. The discharge of TMs has significant correlations to total nitrogen (TN) and total phosphorus (TP); however, no significant correlations were observed between the content of PAHs and TMs. Toxicity effect results show that sediment in the Wujingang River basin threatens sediment-dwelling organisms. The harmful effect was mainly caused by heavy metals especially Cd, Cr, Ni, and Cu. Sediment dredging is an effective way to control pollution from internal rivers especially for the pollution of TN and heavy metals in the Wujingang River basin.
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Functional imaging of implicit marijuana associations during performance on an Implicit Association Test (IAT).
Behav. Brain Res.
PUBLISHED: 06-13-2013
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This research evaluated the neural correlates of implicit associative memory processes (habit-based processes) through the imaging (fMRI) of a marijuana Implicit Association Test. Drug-related associative memory effects have been shown to consistently predict level of drug use. To observe differences in neural activity of associative memory effects, this study compared 13 heavy marijuana users and 15 non-using controls, ranging in age from 18 to 25, during performance of a marijuana Implicit Association Test (IAT). Group by condition interactions in the putamen, caudate, and right inferior frontal gyrus were observed. Relative to non-users, marijuana users showed greater bilateral activity in the dorsal striatum (caudate and putamen) during compatible trials focused on perceived positive outcomes of use. Alternatively, relative to the marijuana-using group, the non-users showed greater activity in the right inferior frontal gyrus during incompatible trials, which require more effortful processing of information. Further, relative to fixation, heavy users showed bilateral activity in the caudate and putamen, hippocampus and some frontal regions during compatible trials and no significant activity during incompatible trials. The non-using group showed greater activity in frontal regions during incompatible trials relative to fixation and no significant activity during compatible trials. These findings are consistent with a dual process framework of appetitive behaviors proposing that (1) implicit associations underlying habit are mediated through neural circuitry dependent on the striatum, and (2) deliberative/controlled behaviors are mediated through circuitry more dependent on the prefrontal cortex.
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In developing our video relationships, we compare around 5 million PubMed articles to our library of over 4,500 methods videos. In some cases the language used in the PubMed abstracts makes matching that content to a JoVE video difficult. In other cases, there happens not to be any content in our video library that is relevant to the topic of a given abstract. In these cases, our algorithms are trying their best to display videos with relevant content, which can sometimes result in matched videos with only a slight relation.