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Find video protocols related to scientific articles indexed in Pubmed.
Cardiac resynchronization therapy with right ventricular sense triggered left ventricular pacing for chronic congestive heart failure.
Cardiol J
PUBLISHED: 10-30-2014
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To investigate the effect of cardiac resynchronization therapy (CRT) with right ventricular sense triggered left ventricular pacing for chronic heart failure (CHF).
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Endometrial stromal cells and decidualized stromal cells: origins, transformation and functions.
Gene
PUBLISHED: 08-26-2014
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Decidualization of endometrium, which is characterized by endometrial stromal cell (ESC) decidualization, vascular reconstruction, immune cell recruitment, and plentiful molecule production, is a crucial step for uterus to become receptive for embryo. When implantation takes place, ESCs surround and directly interact with embryo. Decidualized stromal cells (DSCs) are of great importance in endometrial decidualization, having a broad function in regulating immune activity and vascular remodeling of uterus. DSCs are shown to have a higher metabolic level and looser cytoskeleton than ESCs. What's the origin of ESCs and how ESCs successfully transform into DSCs had puzzled scientists in the last decades. Breakthrough had been achieved recently, and many studies had elucidated some of the characters and functions of DSCs. However, several questions still remain unclear. This paper reviews current understanding of where ESCs come from and how ESCs differentiate into DSCs, summarizes some characters and functions of DSCs, analyzes current studies and their limitations and points out research areas that need further investigation.
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[Effect of SU11248 on leukemia cell line K562 and its molecular mechanisms].
Zhongguo Shi Yan Xue Ye Xue Za Zhi
PUBLISHED: 08-19-2014
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This study was aimed to investigate the effect of SU11248 on proliferation and apoptosis of leukemia cell line K562 in vitro and its mechanism. The inhibitory effect of 3.2 µg/ml SU11248 on K562 proliferation was tested by MTT assay. The ability of SU11248 to induce apoptosis of K562 cells was examined by TUNEL and DNA ladder. The expression of C-MYC, hTERT and BCR-ABL mRNA in K562 cells was detected by RT-PCR. The protein expression of Akt and p-Akt in K562 cells was detected by Western blot. The results showed that the proliferation of K562 cells was obviously inhibited by 3.2 µg/ml SU11248 in a time-dependent manner. SU11248 could induce K562 cells apoptosis in dose-and time-dependent manner. The mRNA expression of C-MYC, hTERT and BCR-ABL was reduced significantly by SU11248 in a time-dependent manner (P < 0.05). Western blot detection showed that the expression of p-Akt protein in K562 cells decreased in dose-and time-dependent manner after SU11248 treatment, but the expression of Akt was not significantly changed. It is concluded that SU11248 can inhibit the growth of K562 cells efficiently through inducing apoptosis, its mechanism may be closely relate with the expression down-regulation of C-MYC, hTERT, BCR-ABL and the inhibition of Akt phosphorylation.
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Maternal exposure to the production of fireworks and reduced rate of new onset hypertension in pregnancy.
Hypertens Pregnancy
PUBLISHED: 07-28-2014
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Carbon monoxide (CO) is one of the main substances contained in fireworks. Previous studies suggested that CO may have protective effect on the development of hypertension of pregnancy.
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Complete mitochondrial genome of Anguilla japonica (Anguilliformes, Anguillidae).
Mitochondrial DNA
PUBLISHED: 07-11-2014
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Abstract The complete mitochondrial genome of Anguilla japonica have been determined in this study. The gene composition, arrangement and transcriptional orientation in A. japonica mitogenome were identical to those of most vertebrates. The complete mitogenome of A. japonica was 16,615?bp in size wih 13 protein-coding genes, 22 tRNA genes, 2 rRNA genes and a control region. Two start codon patterns and three stop codon patterns were found in protein-coding genes. Only the tRNA-Ser2 could not fold into a typical clover-leaf secondary structure for lacking the dihydrouridine arm. Sequences alignment results suggest that the complete mitogenome of A. japonica is an efficient tool to study molecular phylogenetics, biogeography and adaptive evolution of this lineage.
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miR-141 targets ZEB2 to suppress HCC progression.
Tumour Biol.
PUBLISHED: 06-04-2014
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Hepatocellular carcinoma (HCC) is one of the leading causes of cancer-related deaths worldwide. Increasing evidence suggests that microRNAs (miRNAs) are associated with HCC tumorigenesis. The present study was designed to define the role of miR-141 in HCC. The expression of miR-141 was significantly decreased in four HCC cell lines. Overexpression of miR-141 suppressed both the growth and the motility of HCC cells. Furthermore, we identified zinc finger E-box binding homeobox 2 (ZEB2) as a target of miR-141 and miR-141 functioned as a tumor suppressor via ZEB2 targeting in HCC. These data provide a novel potential therapeutic target for HCC treatment.
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[Helicobacter pylori infection and gastric mucosa change and blood-lipid in people undergoing the physical examination in Changsha].
Zhong Nan Da Xue Xue Bao Yi Xue Ban
PUBLISHED: 04-22-2014
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To explore the relationship between the Helicobacter pylori (H.pylori) infection and gastric mucosa change and blood-lipid in people undergoing the physical examination in Changsha.
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Bone marrow mesenchymal stem cells ameliorates seawater-exposure-induced acute lung injury by inhibiting autophagy in lung tissue.
Patholog Res Int
PUBLISHED: 03-12-2014
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Seawater drowning can lead to acute lung injury (ALI). Several studies have shown that bone marrow mesenchymal stem cells (BMSC) treatment could attenuate ALI. However, the mechanisms underlying this phenomenon still remain elusive. Therefore, this study aimed to investigate whether BMSC treatment can ameliorate seawater-induced ALI and its underlying mechanisms in a rat model. In this study, arterial blood gas, lung weight coefficient, and TNF-?, and IL-8 in bronchoalveolar lavage fluid (BALF), as well as histopathology examination, were used to detect the lung injury of seawater exposure. Moreover, western blot and RT-PCR were used to explore autophagy in lung tissues. The results demonstrated that seawater exposure induced ALI including impaired arterial blood gas, pulmonary edema, histopathologic changes, and inflammatory response in lung tissues. What is more, these changes were partly ameliorated by BMSC treatment through inhibition of autophagy in lung tissues. The application of BMSC may be a potential effective treatment for seawater-induced ALI.
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Prospective echocardiographic assessment of cardiac structure and function in Chinese persons living with HIV.
Clin. Infect. Dis.
PUBLISHED: 02-25-2014
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Persons living with human immunodeficiency virus (HIV) are at increased risk of developing cardiovascular disease. Few studies have focused on echocardiographic abnormalities in this population.
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Preparation of thermosensitive polymer magnetic particles and their application in protein separations.
J Colloid Interface Sci
PUBLISHED: 02-21-2014
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This paper presents a kind of thermoresponsive polymeric magnetic particles for protein separations. The magnetofluids were directly encapsulated in hollow particles constructed by self-assembly of rod-coil poly(ethylene glycol)-poly(N-isopropylacrylamide)/?-cyclodextrin (PEG-PNIPAM/?-CD) complexes. The resulting particles showed reversible protein absorption/desorption capacity because the reversible thermo-sensitivity of PNIPAM. Above the lower critical solution temperature (LCST) of PNIPAM, these particles showed high absorptive capacities and adsorption was done at lower temperature. The protein-laden particles are readily removed from the feed solution in a magnetic field.
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Thermodynamic and kinetic investigation on the crucial factors affecting adefovir dipivoxil-saccharin cocrystallization.
Pharm. Res.
PUBLISHED: 02-13-2014
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The aim of this study was to perform a thermodynamic and kinetic investigation on the crucial factors affecting the cocrystallization between adefovir dipivoxil (AD) and saccharin (SAC).
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Regulation of sheep ?-TTP by dietary vitamin E and preparation of monoclonal antibody for sheep ?-TTP.
Gene
PUBLISHED: 01-31-2014
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?-Tocopherol transfer protein (?-TTP) is a cytosolic protein that plays an important role in regulating concentrations of plasma ?-tocopherol (the most bio-active form of vitamin E). Despite the central roles that ?-TTP plays in maintaining vitamin E adequacy, we have only recently proved the existence of the ?-TTP gene in sheep and, for the first time, cloned its full-length cDNA. However, the study of sheep ?-TTP is still in its infancy. In the present study, thirty-five local male lambs of Tan sheep with similar initial body weight were randomly divided into five groups and fed with diets supplemented with 0 (control group), 20, 100, 200, 2000IU·sheep(-1)·d(-1) vitamin E for 120 days. At the end of the experiment, the plasma and liver vitamin E contents were analyzed first and then ?-TTP mRNA and protein expression levels were determined by quantitative real-time PCR (qRT-PCR) and Western-blot analysis, respectively. In addition, as no sheep ?-TTP antibody was available, a specific monoclonal antibody (McAb) against the ovine ?-TTP protein was prepared. The effect of vitamin E supplementation was confirmed by the significant changes in the concentrations of vitamin E in the plasma and liver. As shown by qRT-PCR and Western-blot analysis, dietary vitamin E does not affect sheep ?-TTP gene expression, except for high levels of vitamin E supplementation, which significantly increased expression at the protein level. Importantly, the specific sheep anti-?-TTP McAb we generated could provide optimal recognition in ELISA, Western-blot and immunohistochemistry assays, which will be a powerful tool in future studies of the biological functions of sheep ?-TTP.
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Dietary vitamin E affects ?-TTP mRNA levels in different tissues of the Tan sheep.
Gene
PUBLISHED: 01-21-2014
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The ?-tocopherol transfer protein (?-TTP) is a ~32kDa cytosolic protein that plays an important role in the efficient circulation of plasma ?-tocopherol in the body, a factor with great relevance in reproduction. The ?-TTP gene has been studied in a number of tissues; however, its expression and function in some ovine tissues remain unclear. A previous study from our laboratory has demonstrated ?-TTP expression in sheep liver. In the present study we determined whether ?-TTP is expressed in non-liver tissues and investigated the effects of dietary vitamin E on the ?-TTP mRNA levels. Thirty-five male Tan sheep with similar body weight were randomly allocated into five groups and supplemented 0, 20, 100, 200 and 2000IUsheep(-1)day(-1) vitamin E, for four months, respectively. At the end of the study, the animals were slaughtered and tissue samples from the heart, spleen, lung, kidney, longissimus dorsi muscle and gluteus muscle were immediately collected. We found that the ?-TTP gene is expressed in sheep tissues other than the liver. Moreover, dietary vitamin E levels had influenced the expression levels of ?-TTP gene in these tissues in a tissue-specific way. The technique of immunohistochemistry was used to detect ?-TTP in tissues of the heart, spleen, lung, and kidney and we found that ?-TTP was mainly located in the cytoplasm while no ?-TTP immunoreactivity was detected in the cytoplasm of longissimus dorsi and gluteus muscle samples. Importantly, our findings lay the foundation for additional experiments focusing on the absorption and metabolism of vitamin E in tissues other than the liver.
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miR-17 inhibitor suppressed osteosarcoma tumor growth and metastasis via increasing PTEN expression.
Biochem. Biophys. Res. Commun.
PUBLISHED: 01-11-2014
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MicroRNAs (miRNAs) play essential roles in cancer development and progression. Here, we investigated the role of miR-17 in the progression and metastasis of osteosarcoma (OS). miR-17 was frequently increased in OS tissues and cell lines. Inhibition of miR-17 in OS cell lines substantially suppressed cell proliferation, migration, and invasion. Phosphatase and tensin homolog (PTEN) was identified as a target of miR-17, and ectopic expression of miR-17 inhibited PTEN by direct binding to its 3'-untranslated region (3'-UTR). Expression of miR-17 was negatively correlated with PTEN in OS tissues. Together, these findings indicate that miR-17 acts as an oncogenic miRNA and may contribute to the progression and metastasis of OS, suggesting miR-17 as a potential novel diagnostic and therapeutic target of OS.
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An antiretroviral regimen containing 6 months of stavudine followed by long-term zidovudine for first-line HIV therapy is optimal in resource-limited settings: a prospective, multicenter study in China.
Chin. Med. J.
PUBLISHED: 01-04-2014
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An zidovudine (AZT)-substitution regimen containing 24-week stavudine (d4T) followed by long-term AZT for HIV therapy is potential to trade off short-term AZT-related anemia and long-term risks associated with d4T in resource-limited settings. However, evidence is scarce. This study aims to assess the efficacy and safety of AZT-substitution regimen, aiming to find a regimen with better efficacy, less adverse events, and more affordability in resource-limited settings.
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Genetic analysis of Chinese families reveals a novel truncation allele of the retinitis pigmentosa GTPase regulator gene.
Int J Ophthalmol
PUBLISHED: 01-01-2014
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To make comprehensive molecular diagnosis for retinitis pigmentosa (RP) patients in a consanguineous Han Chinese family using next generation sequencing based Capture-NGS screen technology.
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Silencing of the hTERT gene by shRNA inhibits colon cancer SW480 cell growth in vitro and in vivo.
PLoS ONE
PUBLISHED: 01-01-2014
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Human telomerase reverse transcriptase (hTERT) is the key enzyme responsible for synthesizing and maintaining the telomeres on the ends of chromosomes, and it is essential for cell proliferation. This has made hTERT a focus of oncology research and an attractive target for anticancer drug development. In this study, we designed a small interfering RNA (siRNA) targeting the catalytic subunit of hTERT and tested its effects on the growth of telomerase-positive human colon carcinoma SW480 cells in vitro, as well as on the tumorigenicity of these cells in nude mice. Transient and stable transfection of hTERT siRNA into colon cancer SW480 cells suppressed hTERT expression, reduced telomerase activity and inhibited cell growth and proliferation. Knocking down hTERT expression in SW480 tumors xenografted into nude mice significantly slowed tumor growth and promoted tumor cell apoptosis. Our results suggest that hTERT is involved in carcinogenesis of human colon carcinoma, and they highlight the therapeutic potential of a hTERT knock-down approach.
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Thermosensitive AB4 four-armed star PNIPAM-b-HTPB multiblock copolymer micelles for camptothecin drug release.
J Biomater Sci Polym Ed
PUBLISHED: 11-18-2013
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Thermo-sensitive poly(N-isoproplacrylamide)m-block-hydroxyl-terminated polybutadiene-block-poly(N-isoproplacrylamide)m (PNIPAMm-b-HTPB-b-PNIPAMm, m = 1 or 2) block copolymers, AB4 four-armed star multiblock and linear triblock copolymers, were synthesized by ATRP with HTPB as central blocks, and characterization was performed by (1)H NMR, Fourier transform infrared, and size exclusion chromatography. The multiblock copolymers could spontaneously assemble into more regular spherical core-shell nanoscale micelles than the linear triblock copolymer. The physicochemical properties were detected by a surface tension, nanoparticle analyzer, transmission electron microscope (TEM), dynamic light scattering, and UV-vis measurements. The multiblock copolymer micelles had lower critical micelle concentration than the linear counterpart, TEM size from 100 to 120 nm, and the hydrodynamic diameters below 150 nm. The micelles exhibited thermo-dependent size change, with low critical solution temperature of about 33-35 °C. The characteristic parameters were affected by the composition ratios, length of PNIPAM blocks, and molecular architectures. The camptothecin release demonstrated that the drug release was thermo-responsive, accompanied by the temperature-induced structural changes of the micelles. MTT assays were performed to evaluate the biocompatibility or cytotoxicity of the prepared copolymer micelles.
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[Value of percentage of highly fluorescent lymphocytic cells for rapidly assessing septicemia in tumor patients].
Zhonghua Yi Xue Za Zhi
PUBLISHED: 10-16-2013
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To evaluate the value of percentage of highly fluorescent lymphocytic cells (HFLC%) for rapidly assessing septicemia in tumor patients.
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Kirschner wire migration from the right second rib to the right ventricle: a case report.
Chin. J. Traumatol.
PUBLISHED: 10-10-2013
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Migrations of orthopedic wires to cardiovascular system are uncommon and rarely reported. We report a case of right ventricle embolization with the Kirschner wire that was used for right 2nd rib osteosynthesis 2 years and 8 months previously in a 50-year-old man. The patient was asymptomatic and migration of the Kirschner wire was discovered by routine chest X-ray. An 8 cm-long Kirschner wire was successfully retrieved from the right ventricle. The treatment strategy for Kirschner wire removal from right ventricle is discussed.
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Two-stage model of chemically induced hepatocellular carcinoma in mouse.
Oncol. Res.
PUBLISHED: 09-26-2013
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The aim of this study was to develop an efficient and reproducible mouse model for hepatocellular carcinoma (HCC) research and assess the expression of two proto-oncogenes (c-myc and N-ras) and tumor suppressor gene p53 in the carcinogenic process. In this study, we found that diethylnitrosamine initiation with CCl4 and ethanol promotion could induce a short-term, two-stage liver carcinogenesis model in male BALB/c mice, the process of hepatocarcinogenesis including liver damage, liver necrosis/cell death, liver inflammation, liver proliferation, liver hyperplasia, liver steatosis, and liver cirrhosis and hepatocellular nodules, which mimicked the usual sequence of events observed in human HCC. We also identified that the increase in expression of the p53 gene is related to the proliferation of hepatocytes, whereas overexpression of the c-myc and N-ras genes is associated with hepatocarcinogenesis. This animal model may serve as a basis for recapitulating the molecular pathogenesis of HCC seen in humans.
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[Pre-conceptive factors on the birth ponderal index].
Zhonghua Liu Xing Bing Xue Za Zhi
PUBLISHED: 08-14-2013
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To analyze the pre-conceptive factors on birth ponderal index (PI).
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Thermosensitive PNIPAM-b-HTPB block copolymer micelles: Molecular architectures and camptothecin drug release.
Colloids Surf B Biointerfaces
PUBLISHED: 06-26-2013
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Two kinds of thermo-sensitive poly(N-isoproplacrylamide) (PNIPAM) block copolymers, AB4 four-armed star multiblock and linear triblock copolymers, were synthesized by ATRP with hydroxyl-terminated polybutadiene (HTPB) as central blocks, and characterization was performed by (1)H NMR, FT-IR and SEC. The multiblock copolymers could spontaneously assemble into more regular spherical core-shell nanoscale micelles than the linear triblock copolymer. The physicochemical properties were detected by a surface tension technique, nano particle analyzer, TEM, DLS and UV-vis measurements. The multiblock copolymer micelles had lower critical micelle concentration than the linear counterpart, TEM size from 100 to 120nm and the hydrodynamic diameters below 150nm. The micelles exhibited thermo-dependent size change, with low critical solution temperature about 33-35°C. The characteristic parameters were affected by the composition ratios, length of PNIPAM blocks and molecular architectures. The camptothecin release demonstrated that the drug release was thermo-responsive, accompanied by the temperature-induced structural changes of the micelles. MTT assays were performed to evaluate the biocompatibility or cytotoxicity of the prepared copolymer micelles.
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The Effect of Tai Chi on Chronic Obstructive Pulmonary Disease: A Pilot Randomised Study of Lung Function, Exercise Capacity and Diaphragm Strength.
Heart Lung Circ
PUBLISHED: 06-17-2013
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Although the benefits of exercise on the health of patients with chronic obstructive pulmonary disease (COPD) have been widely reported, the effect of Tai Chi as an alternative exercise has not been thoroughly evaluated in patients with COPD. This study reported a randomised controlled trial, which investigated the effects of Tai Chi on lung function, exercise capacity, and diaphragm strength in patients with COPD.
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[Changes in blood pressure and related determinants before and during normal pregnancy].
Zhonghua Liu Xing Bing Xue Za Zhi
PUBLISHED: 06-14-2013
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To study the longitudinal changes of blood pressure (BP) and its related determinants before and during normal pregnancy.
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Gene Expression Profiles at Moxibustioned Site (ST36): A Microarray Analysis.
Evid Based Complement Alternat Med
PUBLISHED: 06-06-2013
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As a major alternative therapy in Traditional Chinese Medicine, it has been demonstrated that moxibustion could generate a series of molecular events in blood, spleen, and brain, and so forth. However, what would happen at the moxibustioned site remained unclear. To answer this question, we performed a microarray analysis with skin tissue taken from the moxibustioned site also Zusanli acupoint (ST36) where 15-minute moxibustion stimulation was administrated. The results exhibited 145 upregulated and 72 downregulated genes which responded immediately under physiological conditions, and 255 upregulated and 243 downregulated genes under pathological conditions. Interestingly, most of the pathways and biological processes of the differentially expressed genes (DEGs) under pathological conditions get involved in immunity, while those under physiological conditions are involved in metabolism.
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[Effects of bisphenol A on OCT4 and SOX2 genes expression in mouse embryonic stem cells].
Zhonghua Yu Fang Yi Xue Za Zhi
PUBLISHED: 05-31-2013
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To explore the effects of bisphenol A (BPA) exposure on toxicity characteristic and OCT4 and SOX2 gene expression of mouse embryonic stem cells (mESC).
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[Clinical application of oral and lip acupuncture].
Zhongguo Zhen Jiu
PUBLISHED: 05-30-2013
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The origin, manipulations, including needling tools, postures of patients, selection of points, sterilization and specific manipulations, indications, instructions and contraindications of oral and lip acupuncture are sorted and summarized. It lays foundation to the national standard of oral acupuncture manipulations. It is also supposed to be adopted as clinical practice standard, and is going to be popularized in clinics.
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[Zebrafish as the model system to study organogenesis and regeneration].
Yi Chuan
PUBLISHED: 05-11-2013
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Zebrafish (Danio rerio) is becoming more and more popular as a model organism to study vertebrate organogenesis and regeneration, taking the advantage of its extra-uterine development, transparency, strong regeneration ability and suitability for the large scale genetic screen. In the past decades, a series of useful methods have been established in zebrafish, which allows studies of important questions in developmental biology including cell fate determination, differentiation, morphogenesis, and regeneration after tissue/organ injury. Recently, using large scale mutagenesis screen and improved live imaging techniques, many questions that had not been well understood in mice have been successfully investigated in zebrafish. Therefore, we are confident that the zebrafish model system will further help decipher the cellular and molecular mechanisms of organogenesis and regeneration in the future, providing valuable references for the treatment of related clinical diseases. This review just summarized the progress in the studies of the important organs development using zebrafish, such as central nervous system(CNS), liver and pancreas, blood and vessel, recently. Meanwhile, the updated informations about the regeneration of tail fin, heart, liver, retina and so on in zebrafish were also included in this paper.
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Thermosensitive mPEG-b-PA-g-PNIPAM Comb Block Copolymer Micelles: Effect of Hydrophilic Chain Length and Camptothecin Release Behavior.
Pharm. Res.
PUBLISHED: 04-17-2013
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Block copolymer micelles are extensively used as drug controlled release carriers, showing promising application prospects. The comb or brush copolymers are especially of great interest, whose densely-grafted side chains may be important for tuning the physicochemical properties and conformation in selective solvents, even in vitro drug release. The purpose of this work was to synthesize novel block copolymer combs via atom transfer radical polymerization, to evaluate its physicochemical features in solution, to improve drug release behavior and to enhance the bioavailablity, and to decrease cytotoxicity.
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Effect of activated autologous monocytes/macrophages on wound healing in a rodent model of experimental diabetes.
Diabetes Res. Clin. Pract.
PUBLISHED: 04-09-2013
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We aimed to evaluate the effectiveness of the application of activated autologous monocytes/macrophages (Mo/Mp) on wound healing in diabetic rats.
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Targeted intraceptor nanoparticle therapy reduces angiogenesis and fibrosis in primate and murine macular degeneration.
ACS Nano
PUBLISHED: 03-20-2013
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Monthly intraocular injections are widely used to deliver protein-based drugs that cannot cross the blood-retina barrier for the treatment of leading blinding diseases such as age-related macular degeneration (AMD). This invasive treatment carries significant risks, including bleeding, pain, infection, and retinal detachment. Further, current therapies are associated with a rate of retinal fibrosis and geographic atrophy significantly higher than that which occurs in the described natural history of AMD. A novel therapeutic strategy which improves outcomes in a less invasive manner, reduces risk, and provides long-term inhibition of angiogenesis and fibrosis is a felt medical need. Here we show that a single intravenous injection of targeted, biodegradable nanoparticles delivering a recombinant Flt23k intraceptor plasmid homes to neovascular lesions in the retina and regresses CNV in primate and murine AMD models. Moreover, this treatment suppressed subretinal fibrosis, which is currently not addressed by clinical therapies. Murine vision, as tested by OptoMotry, significantly improved with nearly 40% restoration of visual loss induced by CNV. We found no evidence of ocular or systemic toxicity from nanoparticle treatment. These findings offer a nanoparticle-based platform for targeted, vitreous-sparing, extended-release, nonviral gene therapy.
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Soluble vascular endothelial growth factor receptor 3 is essential for corneal alymphaticity.
Blood
PUBLISHED: 03-08-2013
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Corneal transparency is a prerequisite for optimal vision and in turn relies on an absence of blood and lymphatic vessels, which is remarkable given the corneas proximity to vascularized tissues. Membrane-bound vascular endothelial growth factor receptor 3 (VEGFR-3), with its cognate ligand vascular endothelial growth factor C (VEGF-C), is a major mediator of lymphangiogenesis. Here, we demonstrate that the cornea expresses a novel truncated isoform of this molecule, soluble VEGFR-3 (sVEGFR-3), which is critical for corneal alymphaticity, by sequestering VEGF-C. sVEGFR-3 binds and sequesters VEGF-C, thereby blocking signaling through VEGFR-3 and suppressing lymphangiogenesis induced by VEGF-C. sVEGFR-3 knockdown leads to lymphangiogenesis and hemangiogenesis in the mouse cornea, while overexpression of sVEGFR-3 inhibits lymphangiogenesis and hemangiogenesis in a murine suture injury model. Pax6(+/-) mice spontaneously develop corneal and lymphatic vessels and are deficient in sVEGFR-3. sVEGFR-3 suppresses hemangiogenesis by blocking VEGF-C-induced phosphorylation of VEGFR-2. Overexpression of sVEGFR-3 leads to a 5-fold increase in corneal transplant survival in mouse models. sVEGFR-3 holds promise as a molecule to control and regress lymphatic-vessel-based dysfunction. Therefore, sVEGFR-3 has the potential to protect the injured cornea from opacification secondary to infection, inflammation, or transplant rejection.
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Precision frequency metrology of helium 2(1)S(0)?2(1)P(1) transition.
Phys. Rev. Lett.
PUBLISHED: 03-06-2013
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We report a precision frequency measurement of the (4)He 2(1)S(0)?2(1)P(1) transition at 2058 nm. The saturated absorption spectroscopy is performed in a rf discharge sealed-off cell with a volume Bragg grating-based Tm:Ho:YLF laser. The absolute transition frequency measured using a fiber optical frequency comb is 145?622?892?822 (183) kHz with a relative uncertainty of 1.3×10(-9). Our result is ten times more precise than current best theoretical calculations and is in reasonable agreement with the calculated values. However, the ionization energy of the 2(1)P(1) state, derived from our result and other precisely measured transitions, shows a discrepancy of approximately 3.5? with the most precise atomic theory. We have also determined the isotope shift between (3)He and (4)He to be 4248.7 (5.3) MHz, which is more precise than the previous measurement by one order of magnitude.
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Metal-enhanced fluorescence of nano-core-shell structure used for sensitive detection of prion protein with a dual-aptamer strategy.
Anal. Chim. Acta
PUBLISHED: 02-26-2013
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Metal-enhanced fluorescence (MEF) as a newly recognized technology is widespread throughout biological research. The use of fluorophore-metal interactions is recognized to be able to alleviate some of fluorophore photophysical constraints, favorably increase both the fluorophore emission intensity and photostability. In this contribution, we developed a novel metal-enhanced fluorescence (MEF) and dual-aptamer-based strategy to achieve the prion detection in solution and intracellular protein imaging simultaneously, which shows high promise for nanostructure-based biosensing. In the presence of prion protein, core-shell Ag@SiO2, which are functionalized covalently by single stranded aptamer (Apt1) of prions and Cyanine 3 (Cy3) decorated the other aptamer (Apt2) were coupled together by the specific interaction between prions and the anti-prion aptamers in solution. By adjusting shell thickness of the pariticles, a dual-aptamer strategy combined MEF can be realized by the excitation and/or emission rates of Cy3. It was found that the enhanced fluorescence intensities followed a linear relationship in the range of 0.05-0.30 nM, which is successfully applied to the detection of PrP in mice brain homogenates.
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Tumor-specific cytolysis caused by an E1B55K-attenuated adenovirus in nasopharyngeal carcinoma is augmented by cisplatin.
Anat Rec (Hoboken)
PUBLISHED: 02-13-2013
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An E1B55K-attenuated adenovirus, dl1520, has been shown to replicate selectively in and lyse tumor cells. In this study, the antitumor activities of dl1520, alone or in combination with the chemotherapeutic agent cisplatin, were investigated in nasopharyngeal carcinoma (NPC) cells. The results demonstrated that dl1520 replicated in and destroyed NPC cells, and induced apoptosis in vitro. In a nude mouse xenograft model, dl1520 significantly inhibited the growth of NPC cell xenografts, and the viral replication was associated with tumor regression. Importantly, the antitumor activity of dl1520 was augmented by the addition of cisplatin both in vitro and in vivo, showing that dl1520 and cisplatin have a synergistic anti-NPC effect. These data suggest that dl1520 exerts an efficient anti-NPC activity through oncolysis and the induction of apoptosis, which is enhanced synergistically by cisplatin. These findings indicate that oncolytic viral therapeutics using the E1B55K-attenuated adenovirus dl1520 could be promising in the comprehensive treatment of NPC, especially in combination with platinum-based chemotherapy.
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Diversity of methanogens in the hindgut of captive white rhinoceroses, Ceratotherium simum.
BMC Microbiol.
PUBLISHED: 01-28-2013
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The white rhinoceros is on the verge of extinction with less than 20,200 animals remaining in the wild. In order to better protect these endangered animals, it is necessary to better understand their digestive physiology and nutritional requirements. The gut microbiota is nutritionally important for herbivorous animals. However, little is known about the microbial diversity in the gastrointestinal tract (GIT) of the white rhinoceros. Methanogen diversity in the GIT may be host species-specific and, or, function-dependent. To assess methanogen diversity in the hindgut of white rhinoceroses, an archaeal 16S rRNA gene clone library was constructed from pooled PCR products obtained from the feces of seven adult animals.
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Nuclear factors: roles related to mitochondrial deafness.
Gene
PUBLISHED: 01-28-2013
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Hearing loss (HL) is a common disorder with mitochondrial dysfunction as one of the major causes leading to deafness. Mitochondrial dysfunction may be caused by either mutations in nuclear genes leading to defective nuclear-encoded proteins or mutations in mitochondrial genes leading to defective mitochondrial-encoded products. The specific nuclear genes involved in HL can be classified into two categories depending on whether mitochondrial gene mutations co-exist (modifier genes) or not (deafness-causing genes). TFB1M, MTO1, GTPBP3, and TRMU are modifier genes. A mutation in any of these modifier genes may lead to a deafness phenotype when accompanied by the mitochondrial gene mutation. OPA1, TIMM8A, SMAC/DIABLO, MPV17, PDSS1, BCS1L, SUCLA2, C10ORF2, COX10, PLOG1and RRM2B are deafness-causing genes. A mutation in any of these deafness-causing genes will directly induce variable phenotypic HL.
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Photoreceptor avascular privilege is shielded by soluble VEGF receptor-1.
Elife
PUBLISHED: 01-01-2013
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Optimal phototransduction requires separation of the avascular photoreceptor layer from the adjacent vascularized inner retina and choroid. Breakdown of peri-photoreceptor vascular demarcation leads to retinal angiomatous proliferation or choroidal neovascularization, two variants of vascular invasion of the photoreceptor layer in age-related macular degeneration (AMD), the leading cause of irreversible blindness in industrialized nations. Here we show that sFLT-1, an endogenous inhibitor of vascular endothelial growth factor A (VEGF-A), is synthesized by photoreceptors and retinal pigment epithelium (RPE), and is decreased in human AMD. Suppression of sFLT-1 by antibodies, adeno-associated virus-mediated RNA interference, or Cre/lox-mediated gene ablation either in the photoreceptor layer or RPE frees VEGF-A and abolishes photoreceptor avascularity. These findings help explain the vascular zoning of the retina, which is critical for vision, and advance two transgenic murine models of AMD with spontaneous vascular invasion early in life. DOI:http://dx.doi.org/10.7554/eLife.00324.001.
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Progress on materials and scaffold fabrications applied to esophageal tissue engineering.
Mater Sci Eng C Mater Biol Appl
PUBLISHED: 01-01-2013
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The mortality rate from esophageal disease like atresia, carcinoma, tracheoesophageal fistula, etc. is increasing rapidly all over the world. Traditional therapies such as surgery, radiotherapy or chemotherapy have been met with very limited success resulting in reduced survival rate and quality of patients life. Tissue-engineered esophagus, a novel substitute possessing structure and function similar to native tissue, is believed to be an effective therapy and a promising replacement in the future. However, research on esophageal tissue engineering is still at an early stage. Considerable research has been focused on developing ideal scaffolds with optimal materials and methods of fabrication. This article gives a review of materials and scaffold fabrications currently applied in esophageal tissue engineering research.
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Aptamer-conjugated and drug-loaded acoustic droplets for ultrasound theranosis.
Biomaterials
PUBLISHED: 11-03-2011
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Tumor therapy requires multi-functional treatment strategies with specific targeting of therapeutics to reduce general toxicity and increase efficacy. In this study we fabricated and functionally tested aptamer-conjugated and doxorubicin (DOX)-loaded acoustic droplets comprising cores of liquid perfluoropentane compound and lipid-based shell materials. Conjugation of sgc8c aptamers provided the ability to specifically target CCRF-CEM cells for both imaging and therapy. High-intensity focused ultrasound (HIFU) was introduced to trigger targeted acoustic droplet vaporization (ADV) which resulted in both mechanical cancer cell destruction by inertial cavitation and chemical treatment through localized drug release. HIFU insonation showed a 56.8% decrease in cell viability with aptamer-conjugated droplets, representing a 4.5-fold increase in comparison to non-conjugated droplets. In addition, the fully-vaporized droplets resulted in the highest DOX uptake by cancer cells, compared to non-vaporized or partially vaporized droplets. Optical studies clearly illustrated the transient changes that occurred upon ADV of droplet-targeted CEM cells, and B-mode ultrasound imaging revealed contrast enhancement by ADV in ultrasound images. In conclusion, our fabricated droplets functioned as a hybrid chemical and mechanical strategy for the specific destruction of cancer cells upon ultrasound-mediated ADV, while simultaneously providing ultrasound imaging capability.
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[The effect of erythromycin on transforming growth factor-?(1) and secretory leukocyte proteinase inhibitor in a rat model of chronic obstructive pulmonary disease].
Zhonghua Jie He He Hu Xi Za Zhi
PUBLISHED: 11-02-2011
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To study the protective mechanism of erythromycin in the process of COPD.
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Molecular cloning and characterization of the sheep ?-TTP gene and its expression in response to different vitamin E status.
Gene
PUBLISHED: 10-21-2011
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The ?-tocopherol transfer protein (?-TTP) is a ~32 kDa protein that exhibits a marked ligand specificity and selectively recognizes of ?-tocopherol, which is the most active form of vitamin E. The ?-TTP gene has been cloned and its physiological functions have been studied in numbers of species, however, the understanding of sheep ?-TTP is still in his infancy. In this study, the full-length cDNA of sheep ?-TTP gene was cloned from sheep liver by using of rapid amplification of complementary DNA ends (RACE). As a result, the sheep ?-TTP gene was 1098 bp in nucleotide which contained 23 bp 5-untranslated region (UTR), 226 bp 3-UTR and 849 bp open reading frame (ORF) that encoded a basic protein of 282 amino acids. Further bioinformatic analysis indicated that the sheep ?-TTP gene had a high homologous of both nucleotide and amino acid sequences compared with that of other species and had a Sec14p-like lipid-binding domain which called the CRAL-TRIO domain. Moreover, the expression of sheep ?-TTP mRNA and protein in response to different vitamin E supplemented levels were observed according to quantitative real-time PCR (qRT-PCR) and Western blotting analysis. The results showed that dietary vitamin E levels did not affect ?-TTP mRNA expression significantly while the low vitamin E supplemented level groups of sheep had significantly higher ?-TTP protein compared to high-vitamin E groups.
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Preliminary study on the mechanism of acupoint injection of bone marrow mesenchymal stem cells in improving blood flow in the rat of hind limb ischemia.
J Tradit Chin Med
PUBLISHED: 10-08-2011
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To explore the mechanism of acupoint injection of bone marrow mesenchymal stem cells (BM-MSCs) in improving blood flow in the rat with hind limb ischemia.
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catena-Poly[[(diaqua-calcium)-bis-(?-2-fluorobenzoato)-1:1?O:O,O;1:1?O,O:O] 2,2-bipyridine hemi-solvate].
Acta Crystallogr Sect E Struct Rep Online
PUBLISHED: 10-07-2011
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In the title compound, {[Ca(C(7)H(4)FO(2))(2)(H(2)O)(2)]·0.5C(10)H(8)N(2)}(n), the Ca(II) atom is coordinated by eigth O atoms from four 2-fluoro-benzoate ligands and two water mol-ecules, resulting in a distorted CaO(8) square-anti-prismatic coordination environment. The 2-fluoro-benzoate ligand bridges two symmetry-related Ca(II) atoms, giving rise to a chain structure extending along [100]. The distances between the Ca atom and its two symmetry-related counterparts are 4.054?(2) and 4.106?(2)?Å. The polymeric chains are connected by classical O-H?N hydrogen bonds into a layer structure parallel to (010). The layers are connected by non-classical C-H?F hydrogen bonds into a three-dimensional supra-molecular structure. O-H?O and C-H?O inter-actions also occur. The uncoordinated 2,2-bipyridine mol-ecule is located on a centre of symmetry at the mid-point of the bond between the two heterocycles. One of the two benzene rings is disordered over two sites with occupancy factors of 0.60 and 0.40.
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Release of photoactivatable drugs from plasmonic nanoparticles for targeted cancer therapy.
ACS Nano
PUBLISHED: 10-03-2011
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Chemotherapy is an important modality in cancer treatment. The major challenges of recent works are to improve drug loading, increase selectivity to target cells, and control the precise release of drugs. In the present study, we devised a smart drug carrier, an aptamer/hairpin DNA-gold nanoparticle (apt/hp-Au NP) conjugate for targeted delivery of drugs. The DNA aptamer sgc8c, which possesses strong affinity for protein tyrosine kinase 7 (PTK7), abundantly expressed on the surface of CCRF-CEM (T-cell acute lymphoblastic leukemia) cells, was assembled onto the surface of Au NPs. The repeated d(CGATCG) sequence within the hpDNA on the Au NP surface was used for the loading of the anticancer drug doxorubicin (Dox). After optimization, 25 (±3) sgc8c and 305 (±9) Dox molecules were successfully loaded onto the AuNP (13 nm) surface. The binding capability of apt/hp-Au NP conjugates toward targeted cells was investigated by flow cytometry and atomic absorption spectroscopy, which showed that the aptamer-functionalized nanoconjugates were selective for targeting of cancer cells. A cell toxicity (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, MTT) assay also demonstrated that these drug-loaded nanoconjugates could kill targeted cancer cells more effectively than nontargeted (control) cells. Most importantly, when illuminated with plasmon-resonant light (532 nm), Dox:nanoconjugates displayed enhanced antitumor efficacy with few side effects. The marked release of Dox from these nanoconjugates in living cells was monitored by increasing fluorescence signals upon light exposure. In vitro studies confirmed that aptamer-functionalized hp-Au NPs can be used as carriers for targeted delivery of drugs with remote control capability by laser irradiation with high spatial/temporal resolution.
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Retinoid receptors trigger neuritogenesis in retinal degenerations.
FASEB J.
PUBLISHED: 09-22-2011
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Anomalous neuritogenesis is a hallmark of neurodegenerative disorders, including retinal degenerations, epilepsy, and Alzheimers disease. The neuritogenesis processes result in a partial reinnervation, new circuitry, and functional changes within the deafferented retina and brain regions. Using the light-induced retinal degeneration (LIRD) mouse model, which provides a unique platform for exploring the mechanisms underlying neuritogenesis, we found that retinoid X receptors (RXRs) control neuritogenesis. LIRD rapidly triggered retinal neuron neuritogenesis and up-regulated several key elements of retinoic acid (RA) signaling, including retinoid X receptors (RXRs). Exogenous RA initiated neuritogenesis in normal adult retinas and primary retinal cultures and exacerbated it in LIRD retinas. However, LIRD-induced neuritogenesis was partly attenuated in retinol dehydrogenase knockout (Rdh12(-/-)) mice and by aldehyde dehydrogenase inhibitors. We further found that LIRD rapidly increased the expression of glutamate receptor 2 and ? Ca(2+)/calmodulin-dependent protein kinase II (?CaMKII). Pulldown assays demonstrated interaction between ?CaMKII and RXRs, suggesting that CaMKII pathway regulates the activities of RXRs. RXR antagonists completely prevented and RXR agonists were more effective than RA in inducing neuritogenesis. Thus, RXRs are in the final common path and may be therapeutic targets to attenuate retinal remodeling and facilitate global intervention methods in blinding diseases and other neurodegenerative disorders.
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The generation of the endothelial specific cdc42-deficient mice and the effect of cdc42 deletion on the angiogenesis and embryonic development.
Chin. Med. J.
PUBLISHED: 09-17-2011
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High microvascular permeability plays an essential role in pathological process of multiple diseases such as septic shock, acute lung injury and acute respiratory distress syndrome, and burns. Inhibiting hyperpermeability is significant for controlling these conditions. Cdc42, as a main member of the small Rho GTPase family, plays a critical role in controlling and regulating the endothelial junctional permeability. We aimed to generate and identify endothelial specific cdc42-deficient mice by the Cre/loxp recombination approach, for examination in an animal model of the contribution of the cdc42 gene in the microvascular barrier function.
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[Change in body compositions in female patients with human immunodeficiency virus related lipodystrophy syndrome].
Zhongguo Yi Xue Ke Xue Yuan Xue Bao
PUBLISHED: 09-13-2011
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To study the changes of body composition in females patients with human immunodeficiency virus (HIV)-related lipodystrophy (LD) syndrome (HIV-LD).
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Increased expression of multifunctional serine protease, HTRA1, in retinal pigment epithelium induces polypoidal choroidal vasculopathy in mice.
Proc. Natl. Acad. Sci. U.S.A.
PUBLISHED: 08-15-2011
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Age-related macular degeneration (AMD) is the leading cause of irreversible blindness in the elderly. Wet AMD includes typical choroidal neovascularization (CNV) and polypoidal choroidal vasculopathy (PCV). The etiology and pathogenesis of CNV and PCV are not well understood. Genome-wide association studies have linked a multifunctional serine protease, HTRA1, to AMD. However, the precise role of HTRA1 in AMD remains elusive. By transgenically expressing human HTRA1 in mouse retinal pigment epithelium, we showed that increased HTRA1 induced cardinal features of PCV, including branching networks of choroidal vessels, polypoidal lesions, severe degeneration of the elastic laminae, and tunica media of choroidal vessels. In addition, HTRA1 mice displayed retinal pigment epithelium atrophy and photoreceptor degeneration. Senescent HTRA1 mice developed occult CNV, which likely resulted from the degradation of the elastic lamina of Bruchs membrane and up-regulation of VEGF. Our results indicate that increased HTRA1 is sufficient to cause PCV and is a significant risk factor for CNV.
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Effect of bamboo biochar on pentachlorophenol leachability and bioavailability in agricultural soil.
Sci. Total Environ.
PUBLISHED: 08-09-2011
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In this study, bamboo biochar (BBC) was proposed as a practical carbonaceous material for the in situ remediation of organic pollutants. Column leaching experiments were used to evaluate the leachability of pentachlorophenol (PCP) in soil amended with BBC (0, 1, 2, and 5%; w/w). After leaching, the residual bioavailability of PCP in the soil column was determined by chemical extraction methods (methanol and distilled water). The results showed that compared to the control, the addition of 5% BBC decreased the cumulative leach-loss content of PCP by 42%, and also reduced PCP concentrations by 56 and 65% in methanol and distilled water extracts, respectively. Moreover, the linear leaching curves for the 10-h leaching time indicated that the rapid release process may be dependent on the continuous diffusion and partition mechanism. Regression analyses showed a significant (p<0.001) relationship between the BBC percentage and cumulative content of leached PCP, in addition, a significant relationship between BBC percentage and the chemical extraction of PCP with correlation coefficients (R(2)) greater than 0.9. Therefore, adding BBC to soil could prevent PCP further contamination such as bioavailability, surface or groundwater contamination through leaching.
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[Endoplasmic reticulum stress and related apoptosis in the lungs of a chronic obstructive pulmonary disease rat model].
Zhonghua Jie He He Hu Xi Za Zhi
PUBLISHED: 07-07-2011
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To study the endoplasmic reticulum stress (ERS) and the apoptosis of alveolar epithelial cells in a COPD rat model.
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Sensitization of epithelial growth factor receptors by nicotine exposure to promote breast cancer cell growth.
Breast Cancer Res.
PUBLISHED: 06-30-2011
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Tobacco smoke is known to be the main cause of lung, head and neck tumors. Recently, evidence for an increasing breast cancer risk associated with tobacco smoke exposure has been emerging. We and other groups have shown that nicotine, as a non-conventional carcinogen, has the potential to facilitate cancer genesis and progression. However, the underlying mechanisms by which the smoke affects the breast, rather than the lung, remain unclear. Here, we examine possible downstream signaling pathways of the nicotinic acetylcholine receptor (nAChR) and their role in breast cancer promotion.
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[A report of eight cases of Behcets disease with intracardiac thrombus and literatures review].
Zhonghua Nei Ke Za Zhi
PUBLISHED: 06-29-2011
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To analyze the clinical characteristics of Behcets disease with intracardiac thrombus.
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[Change of IL-22R1 expression in human airway smooth muscle cells in response to different stimulating agents].
Nan Fang Yi Ke Da Xue Xue Bao
PUBLISHED: 06-22-2011
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To explore the effects of serum of asthmatic patients, dexamethasone, interleukin-4 (IL-4), interferon-gamma (IFN-?) and transforming growth factor-? (TGF-?) on the expression of interleukin-22 receptor 1 (IL-22R1) mRNA and protein in HASMCs in vitro.
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[Construction of recombinant eukaryotic expression vector pDsRed1-C3/XAPC7 and cellular localization of XAPC7 protein in different cell lines].
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi
PUBLISHED: 05-12-2011
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To construct the recombinant eukaryotic expression vector pDsRed1-C3/XAPC7 and to investigate the cellular localization of XAPC7 protein in 786-O and 293T and Chang liver cell lines.
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[Application of flexirigid thoracoscopy in the diagnosis of pleural disease with unknown etiology].
Nan Fang Yi Ke Da Xue Xue Bao
PUBLISHED: 04-26-2011
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To investigate the diagnostic accuracy of flexirigid thoracoscopy for pleural diseases and the patients compliance.
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Clinical analysis of 68 patients with pulmonary mycosis in China.
Multidiscip Respir Med
PUBLISHED: 04-26-2011
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Due to the lack of specific clinical manifestations and imaging features, the diagnosis of pulmonary mycosis is difficult. This study aimed to investigate the pathogens, clinical manifestations, imaging features, diagnosis and management of pulmonary mycosis.
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[Association of the C3 gene polymorphisms with susceptibility to adult asthma].
Zhonghua Yi Xue Yi Chuan Xue Za Zhi
PUBLISHED: 04-05-2011
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To investigate the association between single nucleotide polymorphisms (SNPs) of the complement component 3 gene (C3) and adult asthma of Hans in southern China.
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[Association of single nucleotide polymorphisms of MD-1 gene with asthma in adults of Han Nationality in Southern China].
Zhonghua Jie He He Hu Xi Za Zhi
PUBLISHED: 03-24-2011
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To investigate the correlation between MD-1 gene single nucleotide polymorphism (SNP) and bronchial asthma in adults of the Han nationality in Southern China as well as their lung functions.
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[Alteration of PTEN gene expression affects the migration of human airway smooth muscle cells in vitro].
Nan Fang Yi Ke Da Xue Xue Bao
PUBLISHED: 03-23-2011
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To investigate the changes in human airway smooth muscle cell (HASMC) migration and related signaling pathway after interference with PTEN gene expression.
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Fabrication and caffeine release from Fe3O4/P(MAA-co-NVP) magnetic microspheres with controllable core-shell architecture.
J Biomater Sci Polym Ed
PUBLISHED: 03-19-2011
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A novel route was proposed to design and construct a magnetic composite microsphere consisting of Fe(3)O(4) nanoparticles chemically-covalently encapsulated with pH-smart poly(methacrylic acid-co-N-vinyl pyrrolidone) (P(MAA-co-NVP)) cross-linked co-polymers by a surface-initiated radical dispersion polymerization route. The multistep surface treatment was employed to improve the dispersity and surface-chemical reactivity of Fe(3)O(4) nanoparticles, involving introduction of active -NH(2) groups, coupling of 1,1-methylene bis-(4-isocyanato-cyclohexane) and immobilization of 2,2-azobis[2-methyl-N-(2-hydroxyethyl) propionamide]. The structure and morphological characterization was carried out by FT-IR, TEM, SEM and XRD. The chemically covalent interactions were investigated by FT-IR, TEM, TGA and DSC. The neat Fe(3)O(4) nanoparticles took on an aggregated spherical shape with an average diameter of about 12 nm, while Fe(3)O(4)/P(MAA-co-NVP) magnetic microspheres assumed controllable and monodispersed spheres with a mean dimension of ca. 0.8 ?m. The microspheres exhibited superparamagnetic properties. The in vitro caffeine release behavior under varying pH environment was investigated to evaluate the potential of Fe(3)O(4)/P(MAA-co-NVP) magnetic microspheres as a magnetic drug targeting carrier. The results indicated that the microspheres have a faster drug-release rate at pH 7.4 than at pH 1.4, corresponding to their pH swelling. The kinetic modeling demonstrated that the drug release is controlled by a balance between co-polymer chain relaxation and Fickian diffusion process, and the proposed carrier is suitable for a magnetic targeting drug-delivery system.
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Impact of black carbon originated from fly ash and soot on the toxicity of pentachlorophenol in sediment.
J. Hazard. Mater.
PUBLISHED: 03-18-2011
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The widely existing fly ash and soot produced during the process of combustion, which are often known as waste but also an important source of black carbon (BC) in the environment, were treated by HCl and HF solution for this study, and recorded as FC and SC, respectively. A series of experiments were carried out to investigate the toxicity of pentachlorophenol (PCP) in sediment, influence of various BCs in sediment with different contents (0%, 0.5%, 1%, 2%, 5% and 10%) on the extractability and toxicity of PCP (50mg/kg), and toxicity of various BC in sediment. The results demonstrated that the PCP exposure to wheat seed exhibited a dose-dependent behavior, and the extractability and toxicity of PCP decreased with the increasing content of BC in sediment. The PCP extractable rate was significantly (P<0.01) influenced by the higher content of BCs. Noticeably, each BC had no toxic but stimulative effect on root elongation and early seedling growth. Furthermore, it was found that the inhibitive effect on the extractability and toxicity of PCP and the stimulative effect on root elongation and early seedling growth caused by SC were more evident than FC.
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Overview of antiretroviral treatment in China: advancement and challenges.
Chin. Med. J.
PUBLISHED: 03-03-2011
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As the severity of the HIV epidemic in China grew, National Free Antiretroviral Treatment (ART) Program was announced since 2003. Even though there still were many difficulties, China had obtained great achievements in fighting against HIV. Over 52 000 adult patients had received first-line HAART thus far and the mortality of AIDS in China decreased significantly. This paper presents an overview of the HIV/AIDS epidemic in China; the status of national free ART program, the difficulties suffered and the achievements made since the initiation of program and the challenges ahead for continued progress for China. This paper also provides suggestions to overcome these challenges.
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[Immune tolerance induced by bone marrow cell transplantation combined with use of cyclophosphamide in diabetic rats with pancreatic transplantation].
Sichuan Da Xue Xue Bao Yi Xue Ban
PUBLISHED: 03-02-2011
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To study the immune tolerance induced by bone marrow cell transplantation combined with short-term use of cyclophosphamide after pancreatic transplantation in diabetic rats.
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The influence of acid demineralization on surface characteristics of black carbon and its sorption for pentachlorophenol.
J Colloid Interface Sci
PUBLISHED: 02-17-2011
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Acid treatment is a routine demineralization process to obtain black carbon (BC), but there has been little systematic research about its influence on BCs characteristics. In this study, elemental analysis, SEM, FTIR, and Boehm titration were used to investigate that effect. Our results showed that the acid treatment had little influence on the sorption of fly ash and soot to pentachlorophenol (PCP), but it greatly increased the sorption of rice chars to PCP. There were two competing effects of acid demineralization on the adsorption capacity of BC. On one hand, it increased the amount of the acidic functional groups, which decreased the adsorption capacity. On the other hand, it increased the surface and pore volume of BC and caused the emergence of hidden carbon enclosed by minerals, which in turn increased the sorptivity significantly. Especially for rice chars (600°C), after acid treatment, their surface area increased from 3.52 to 235 m(2) g(-1) and the sorption capacity coefficient increased from 2.12 to 4.10.
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Characterization and risk assessment of polychlorinated biphenyls in soils and vegetations near an electronic waste recycling site, South China.
Chemosphere
PUBLISHED: 01-29-2011
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This study aimed at identifying the levels of PCBs generated from e-waste recycling, and their potential impacts on the soils and vegetations as well. The ?PCBs concentrations in soil and plant samples ranged from 7.4 to 4000 ng g(-1) and from 6.7 to 1500 ng g(-1), respectively. For the plant samples, Chrysanthemum coronarium L. from vegetable field and the wild plant Bidens pilosa L. from the burning site showed relatively higher PCB concentrations than other species. For the soil samples, the e-waste burning site had relatively higher PCB concentrations than the adjacent areas, and vegetable soils had higher PCB concentrations than paddy soils. The PCB concentrations showed a clear decreasing trend with the increasing distance from the e-waste recycling site. PCB 28, 99, 101, 138, 153, and 180 were the predominant congeners. Principal component analysis results showed a potential fractionation of PCB compositions from the burning site to the surroundings. The PCB congener pattern at the burning site was similar to Arochlor 1260, pointing to an input of non-domestic e-waste. Similar PCB congeners were found in soils and related vegetables, indicating they derived from the same source. The consumption of vegetables grown in soils near e-waste recycling sites should be strictly avoided due to the high PCBs in the plant tissues.
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Effect of trace amounts of polyacrylamide (PAM) on long-term performance of activated sludge.
J. Hazard. Mater.
PUBLISHED: 01-29-2011
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This study aims at evaluating the impacts of PAM addition on activated sludge performance. Four lab-scale sequencing batch reactors (SBRs), each with a working volume of 3L, were investigated with different PAM concentrations. Experiments were conducted with varying organic loading rate and the sludge volume index (SVI), particle size, zeta potential, specific oxygen uptake rate (SOUR), mixed liquor suspended solids (MLSS), COD and ammonium removal efficiency were monitored over a 105-day period. The results showed that all of the PAM addition not only improved the removal efficiencies of COD and ammonium, but also exhibited some advantages on sludge performance. It was found that the sludge performance of settling property, flocculation and microbial activity increased with increasing concentration of PAM. However, high level of PAM (1mg/L) led to the formation of large amounts of loose-structure flocs, which eliminated dissolved oxygen transfer and caused the sludge disintegration, resulting in bad settleability and lower microbial activity. In this way, when the dosage of PAM was 0.1mg/L, the sludge had the best settling property and activity.
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Evaluation of a real-time RT-PCR assay using minor groove binding probe for specific detection of Chinese wild-type classical swine fever virus.
J. Virol. Methods
PUBLISHED: 01-06-2011
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A one-step real-time RT-PCR assay using a minor groove binding probe was developed for the specific detection of Chinese wild-type classical swine fever virus (CSFV). The assay detected wild-type CSFV strains representing different genotypes, but did not amplify viral RNA from the Hog Cholera Lipinized Virus (HCLV) vaccine-strain and other porcine viruses. The assay had a detection limit of 10 copies/reaction or 3.0 median tissue culture infective dose/reaction. In comparison to the sequencing nested RT-PCR assay, the sensitivity and specificity of the assay were 98.3% and 94.3%, respectively, when testing 515 veterinary samples. Wild-type CSFV RNA was detected in nasal swabs 2-4 days before detection in serum samples from pigs exposed to infection by contact, and 2-4 days prior to the onset of clinical disease. HCLV RNA remained undetectable in nasal swabs and serum samples from vaccinated pigs. In conclusion, the novel assay described in this study provides a rapid and sensitive method for differentiating between wild-type and the HCLV-strain of CSFV. It could be used for monitoring in CSF outbreak areas or as a screening method for CSFV eradication strategies.
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JoVE Visualize is a tool created to match the last 5 years of PubMed publications to methods in JoVE's video library.

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In developing our video relationships, we compare around 5 million PubMed articles to our library of over 4,500 methods videos. In some cases the language used in the PubMed abstracts makes matching that content to a JoVE video difficult. In other cases, there happens not to be any content in our video library that is relevant to the topic of a given abstract. In these cases, our algorithms are trying their best to display videos with relevant content, which can sometimes result in matched videos with only a slight relation.