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Find video protocols related to scientific articles indexed in Pubmed.
Human infection with highly pathogenic A(H7N7) avian influenza virus, Italy, 2013.
Emerging Infect. Dis.
PUBLISHED: 10-02-2014
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During an influenza A(H7N7) virus outbreak among poultry in Italy during August-September 2013, infection with a highly pathogenic A(H7N7) avian influenza virus was diagnosed for 3 poultry workers with conjunctivitis. Genetic analyses revealed that the viruses from the humans were closely related to those from chickens on affected farms.
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Risk factors for colonization with carbapenemase-producing Klebsiella pneumoniae in hospital: a matched case-control study.
Am J Infect Control
PUBLISHED: 03-31-2014
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The present case-control study aimed to identify the main factors associated with colonization with carbapenemase-producing Klebsiella pneumoniae (CPKP) in acute care facilities. Administration of carbapenems (odds ratio [OR], 3.67; 95% confidence interval [CI], 1.37-9.83) and other antibiotics (OR, 2.83; 95% CI, 1.10-7.31) during the hospital stay was significantly associated with outcome. The design of this study, with concurrent selection of controls and matching by hospital ward and date of screening, provides a more accurate estimation of relative risks for CPKP colonization than previous investigations.
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The role and utilisation of public health evaluations in Europe: a case study of national hand hygiene campaigns.
BMC Public Health
PUBLISHED: 01-27-2014
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Evaluations are essential to judge the success of public health programmes. In Europe, the proportion of public health programmes that undergo evaluation remains unclear. The European Centre for Disease Prevention and Control sought to determine the frequency of evaluations amongst European national public health programmes by using national hand hygiene campaigns as an example of intervention.
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Feasibility and effectiveness of a low cost campaign on antibiotic prescribing in Italy: community level, controlled, non-randomised trial.
BMJ
PUBLISHED: 09-14-2013
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To test the hypothesis that a multifaceted, local public campaign could be feasible and influence antibiotic prescribing for outpatients.
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Global implementation of WHOs multimodal strategy for improvement of hand hygiene: a quasi-experimental study.
Lancet Infect Dis
PUBLISHED: 08-23-2013
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Health-care-associated infections are a major threat to patient safety worldwide. Transmission is mainly via the hands of health-care workers, but compliance with recommendations is usually low and effective improvement strategies are needed. We assessed the effect of WHOs strategy for improvement of hand hygiene in five countries.
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Antimicrobial resistance and stewardship in long-term care settings.
Future Microbiol
PUBLISHED: 08-02-2013
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Infections and antimicrobial resistance (AMR) in long-term care facilities (LTCFs) are a public health challenge and a future infectious disease threat. More and more data show the dimension and impact of AMR and of inappropriate use of antimicrobials in this setting. Recently, the spread of carbapenemase-producing Enterobacteriaceae has provided new insights into the dangerous role the long-term care sector may play in the AMR problem in a community. Implementation of effective infection and surveillance control programs in LTCFs is challenging, due to scarce resources (personnel, expertise, diagnostic and supportive services), and no or poor coordination of medical care. However, interventions in LTCFs have been proven to be effective: inappropriate use of antibiotics for asymptomatic bacteriuria may be reduced; hand hygiene compliance may be improved; and the transmission of multidrug-resistant organisms may be halted. This paper reviews the most recent epidemiological information on this issue, providing references to valuable intervention programs.
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Outbreak of Citrobacter freundii carrying VIM-1 in an Italian Hospital, identified during the carbapenemases screening actions, June 2012.
Int. J. Infect. Dis.
PUBLISHED: 02-06-2013
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The identification of patients colonized or infected with carbapenemase-producing Enterobacteriaceae (CPE), in order to control and prevent the global spread of multidrug-resistant (MDR) pathogens.
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Staphylococcus aureus in a northern Italian region: phenotypic and molecular characterization.
Scand. J. Infect. Dis.
PUBLISHED: 09-05-2011
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Staphylococcus aureus is a leading cause of community-acquired infections and healthcare-associated infections. Epidemiological data are useful for understanding the dynamics of the diffusion of this pathogen, and to plan control activities and monitor their efficacy.
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Inflammatory cytokine expression is associated with chikungunya virus resolution and symptom severity.
PLoS Negl Trop Dis
PUBLISHED: 07-05-2011
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The Chikungunya virus infection zones have now quickly spread from Africa to parts of Asia, North America and Europe. Originally thought to trigger a disease of only mild symptoms, recently Chikungunya virus caused large-scale fatalities and widespread economic loss that was linked to recent virus genetic mutation and evolution. Due to the paucity of information on Chikungunya immunological progression, we investigated the serum levels of 13 cytokines/chemokines during the acute phase of Chikungunya disease and 6- and 12-month post-infection follow-up from patients of the Italian outbreak. We found that CXCL9/MIG, CCL2/MCP-1, IL-6 and CXCL10/IP-10 were significantly raised in the acute phase compared to follow-up samples. Furthermore, IL-1?, TNF-?, Il-12, IL-10, IFN-? and IL-5 had low initial acute phase levels that significantly increased at later time points. Analysis of symptom severity showed association with CXCL9/MIG, CXCL10/IP-10 and IgG levels. These data give insight into Chikungunya disease establishment and subsequent convalescence, which is imperative to the treatment and containment of this quickly evolving and frequently re-emerging disease.
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Accuracy of automated and manual systems for susceptibility testing of Pseudomonas aeruginosa to piperacillin and piperacillin-tazobactam.
New Microbiol.
PUBLISHED: 01-15-2011
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This study aimed to evaluate the accuracy of routine systems (Vitek2 cards AST-N022 and AST-N026; Kirby Bauer; Etest) for susceptibility testing of Pseudomonas aeruginosa to piperacillin and piperacillin-tazobactam. Vitek2 (card AST-N022) showed the worst performance; the other three methods (Vitek2 card AST-N026, Kirby-Bauer and E-test) performed comparably but never fulfilled the minimal standard proposed by FDA.
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Neuropathology of the recessive A673V APP mutation: Alzheimer disease with distinctive features.
Acta Neuropathol.
PUBLISHED: 07-30-2010
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Mutations of three different genes, encoding ?-amyloid precursor protein (APP), presenilin 1 and presenilin 2 are associated with familial Alzheimers disease (AD). Recently, the APP mutation A673V has been identified that stands out from all the genetic defects previously reported in these three genes, since it causes the disease only in the homozygous state (Di Fede et al. in Science 323:1473-1477, 2009). We here provide the detailed neuropathological picture of the proband of this family, who was homozygous for the APP A673V mutation and recently came to death. The brain has been studied by histological and immunohistochemical techniques, at the optical and ultrastructural levels. Cerebral A? accumulation and tau pathology were severe and extensive. Peculiar features were the configuration of the A? deposits that were of large size, mostly perivascular and exhibited a close correspondence between the pattern elicited by amyloid stainings and the labeling obtained with immunoreagents specific for A?40 or A?42. Moreover, A? deposition spared the neostriatum while deeply affecting the cerebellum, and therefore was not in compliance with the hierarchical topographical sequence of involvement documented in sporadic AD. Therefore, the neuropathological picture of familial AD caused by the APP recessive mutation A673V presents distinctive characteristics compared to sporadic AD or familial AD inherited as a dominant trait. Main peculiar features are the morphology, structural properties and composition of the A? deposits as well as their topographic distribution in the brain.
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Alzheimers disease and amyloid beta-peptide deposition in the brain: a matter of aging?
Biochem. Soc. Trans.
PUBLISHED: 03-20-2010
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Biomolecules can experience aging processes that limit their long-term functionality in organisms. Typical markers of protein aging are spontaneous chemical modifications, such as AAR (amino acid racemization) and AAI (amino acid isomerization), mainly involving aspartate and asparagine residues. Since these modifications may affect folding and turnover, they reduce protein functionality over time and may be linked to pathological conditions. The present mini-review describes evidence of AAR and AAI involvement in the misfolding and brain accumulation of Abeta (amyloid beta-peptide), a central event in AD (Alzheimers disease) synaptic dysfunctions. Structural alterations introduced by site-specific modifications linked to protein aging may affect Abeta production, polymerization and clearance, and therefore play a pivotal role in the pathogenesis of sporadic and genetic forms of AD. Early changes associated with molecular aging also have significant long-term consequences for Abeta folding and turnover. New fast, reproducible and accurate methods for the screening of protein aging markers in biological samples may contribute to improve diagnostic and therapeutic approaches in AD.
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Chikungunya virus in North-Eastern Italy: a seroprevalence survey.
Am. J. Trop. Med. Hyg.
PUBLISHED: 03-09-2010
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After an outbreak of Chikungunya infection in Emilia-Romagna Region (North-eastern Italy), a survey was performed to estimate the seroprevalence of antibody to Chikungunya virus and the proportion of asymptomatic infections, to identify factors associated with infection, and evaluate the performance of the surveillance system. The method used was a survey on a random sample of residents of the village with the largest number of reported cases. The prevalence was 10.2% (33 of 325), being higher in older people and males, and lower when window screens and insect repellents were used. Only 18% of infected persons were fully asymptomatic, 85% of the 27 symptomatic confirmed cases satisfied the surveillance case definition, and 63% of the persons meeting the criteria for suspect case were identified by the active surveillance system. This study provides basic parameters for modeling the transmission potential of outbreaks and planning control measures for Chikungunya infection in temperate settings.
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Comparison of sales and reimbursement data regarding outpatient antibiotic use in a northern Italian Region.
Pharmacoepidemiol Drug Saf
PUBLISHED: 07-23-2009
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To evaluate the accuracy of systemic antibiotic use statistics, based on the reimbursement data of the Emilia-Romagna Region (Italy), focusing on agents prescribed for urinary tract infections.
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Why do paediatricians prescribe antibiotics? Results of an Italian regional project.
BMC Pediatr
PUBLISHED: 03-27-2009
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To investigate determinants of antibiotic prescription in paediatric care, as a first step of a multilevel intervention to improve prescribing for common respiratory tract infections (RTIs) in a northern Italian region with high antibiotic prescription rate.
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Amyloid beta peptide promotes differentiation of pro-inflammatory human myeloid dendritic cells.
Neurobiol. Aging
PUBLISHED: 02-20-2009
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A key event of Alzheimers disease (AD) pathogenesis is the production of amyloid beta peptides (A beta), which are hypothesized to lead to neurodegeneration by still unclear mechanisms, including a chronic inflammatory response characterized by innate immune cell activation and pro-inflammatory molecule release. Since dendritic cells (DCs) are central players of innate immune response and brain dendritic-like cells may have a crucial role in AD pathogenesis, this study investigates the effects of A beta on human DC functions. Myeloid DCs differentiated in the presence of A beta 42 showed an increase in survival and soluble antigen uptake, a reduction in HLA molecule expression and in IL-10 and IL-12 production. Accordingly, A beta 42-treated DCs were impaired in inducing T cell proliferation and IL-2 production. On the other hand, A beta 42 treatment provided DCs with the ability to release higher levels of IL-1 beta, IL-6 and IL-18, than control DCs. These results demonstrate that A beta 42 can modulate the immune system by inducing pro-inflammatory DC differentiation, thus gaining new insights into AD pathogenesis and immune-based therapeutic intervention.
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APP mutations in the A? coding region are associated with abundant cerebral deposition of A?38.
Acta Neuropathol.
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A? is the main component of amyloid deposits in Alzheimer disease (AD) and its aggregation into oligomers, protofibrils and fibrils is considered a seminal event in the pathogenesis of AD. A? with C-terminus at residue 42 is the most abundant species in parenchymal deposits, whereas A? with C-terminus at residue 40 predominates in the amyloid of the walls of large vessels. A? peptides with other C-termini have not yet been thoroughly investigated. We analysed A?38 in the brains of patients with A? deposition linked to sporadic and familial AD, hereditary cerebral haemorrhage with amyloidosis, or Down syndrome. Immunohistochemistry, confocal microscopy, immunoelectron microscopy, immunoprecipitation and the electrophoresis separation of low molecular weight aggregates revealed that A?38 accumulates consistently in the brains of patients carrying APP mutations in the A? coding region, but was not detected in the patients with APP mutations outside the A? domain, in the patients with presenilin mutations or in subjects with Down syndrome. In the patients with sporadic AD, A?38 was absent in the senile plaques, but it was detected only in the vessel walls of a small subset of patients with severe cerebral amyloid angiopathy. Our results suggest that APP mutations in the A? coding region favour A?38 accumulation in the brain and that the molecular mechanisms of A? deposition in these patients may be different from those active in patients with familial AD associated with other genetic defects and sporadic AD.
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A population based cohort study to assess the safety of pandemic influenza vaccine Focetria in Emilia-Romagna region, Italy - part two.
Vaccine
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A two phases post authorization safety and effectiveness study of individuals vaccinated with the MF59-adjuvanted A/H1N1 influenza vaccine, Focetria (Novartis Vaccines & Diagnostics, Siena, Italy), was conducted in Emilia-Romagna region, Italy during the 2009 A/H1N1 influenza pandemic. The second phase study aim was to detect short- and long-term adverse events of special interest (AESIs) following vaccination, and to measure vaccine effectiveness in term of hospital admissions.
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Good gene, bad gene: new APP variant may be both.
Prog. Neurobiol.
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APP mutations cause Alzheimer disease (AD) with virtually complete penetrance. We found a novel APP mutation (A673V) in the homozygous state in a patient with early-onset AD-type dementia and in his younger sister showing initial signs of cognitive decline. It is noteworthy that the heterozygous relatives were not affected, suggesting that this mutation is inherited as an autosomal recessive trait. Studies on molecular events for the recessive mutation in causing disease revealed a double synergistic effect: the A673V APP variant shifts APP processing towards the amyloidogenic pathway with increased production of A? peptides and it markedly enhances the aggregation and fibrillogenic properties of both A?1-40 and A?1-42. However, co-incubation of mutated and wild-type (wt) A? species resulted in inhibition of amyloidogenesis, consistent with the observation that heterozygous carriers do not develop the disease. The opposite effects of the A673V mutation in the homozygous and heterozygous state on amyloidogenesis account for the autosomal recessive pattern of inheritance, revealing a new scenario in AD genetics and pathogenesis. The anti-amyloidogenic properties of this novel human A? variant may offer grounds for the development of therapeutic strategies for AD based on modified A? peptides. Indeed, the interaction between mutated A?1-6 and wt full-length A? prevents amyloid fibril formation. The anti-amyloidogenic effect is further amplified by the use of a mutated six-mer peptide, constructed entirely from D-amino acids to increase the its stability in vivo. Here we reviewed the studies on pathogenic mechanisms associated with the A673V mutation and the first experimental steps toward the development of a novel disease-modifying therapy for AD.
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What is Visualize?

JoVE Visualize is a tool created to match the last 5 years of PubMed publications to methods in JoVE's video library.

How does it work?

We use abstracts found on PubMed and match them to JoVE videos to create a list of 10 to 30 related methods videos.

Video X seems to be unrelated to Abstract Y...

In developing our video relationships, we compare around 5 million PubMed articles to our library of over 4,500 methods videos. In some cases the language used in the PubMed abstracts makes matching that content to a JoVE video difficult. In other cases, there happens not to be any content in our video library that is relevant to the topic of a given abstract. In these cases, our algorithms are trying their best to display videos with relevant content, which can sometimes result in matched videos with only a slight relation.