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Find video protocols related to scientific articles indexed in Pubmed.
Serotonin 2A receptor regulates microtubule assembly and induces dynamics of dendritic growth cones in rat cortical neurons in vitro.
Neurosci. Res.
PUBLISHED: 03-19-2014
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Serotonin (5-HT) regulates the development of cerebral cortex, but 5-HT receptors mediating the effects are poorly understood. We investigated roles of 5-HT2A receptor in dendritic growth cones using dissociation culture of rat cerebral cortex. Neurons at embryonic day 16 were cultured for 4 days and treated with 5-HT2A/2C receptor agonist (DOI) for 4h. DOI increased the size of growth cone periphery which was actin-rich and microtubule-associated protein 2-negative at the dendritic tip. The length increase of the growth cone periphery may be mediated by 5-HT2A receptor, because the 5-HT2A receptor antagonist reversed the effects of DOI. Moreover, the time-lapse analysis demonstrated the increase of morphological dynamics in dendritic growth cones by DOI. Next, to elucidate the mechanisms underlying the actions of 5-HT2A receptor in dendritic growth cones, we examined the cytoskeletal proteins, tyrosinated ?-tubulin (Tyr-T; dynamic tubulin) and acetylated ?-tubulin (Ace-T; stable tubulin). DOI increased the fluorescence intensity of Tyr-T, while decreased that of Ace-T in the dendritic growth cone periphery. These effects were reversed by the 5-HT2A receptor antagonist, suggesting that 5-HT2A receptor promotes microtubule dynamics. In summary, it was suggested that 5-HT2A receptor induces morphological changes and dynamics of dendritic growth cones through regulation of microtubule assembly.
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JoVE Visualize is a tool created to match the last 5 years of PubMed publications to methods in JoVE's video library.

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We use abstracts found on PubMed and match them to JoVE videos to create a list of 10 to 30 related methods videos.

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In developing our video relationships, we compare around 5 million PubMed articles to our library of over 4,500 methods videos. In some cases the language used in the PubMed abstracts makes matching that content to a JoVE video difficult. In other cases, there happens not to be any content in our video library that is relevant to the topic of a given abstract. In these cases, our algorithms are trying their best to display videos with relevant content, which can sometimes result in matched videos with only a slight relation.