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Find video protocols related to scientific articles indexed in Pubmed.
Nailfold videocapillaroscopy micro-haemorrhage and giant capillary counting as an accurate approach for a steady state definition of disease activity in systemic sclerosis.
Arthritis Res. Ther.
PUBLISHED: 05-15-2014
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IntroductionNailfold videocapillaroscopy (NVC) in systemic sclerosis (SSc) is a procedure commonly used for patient classification and sub-setting, but not to define disease activity (DA). This study aimed to evaluate whether the number of micro-haemorrhages (MHE), micro-thrombosis (MT), giant capillaries (GC), and normal/dilated capillaries (Cs) in NVC could predict DA in SSc.MethodsEight finger NVC was performed in 107 patients with SSc, and the total number of MHE/MT, GC, and the mean number of Cs were counted and defined as number of micro-haemorrhages (NEMO), GC and Cs scores respectively. The European Scleroderma Study Group (ESSG) index constituted the gold standard for DA assessment, and scores ¿3.5 and¿=¿3 were considered indicative of high and moderate activity respectively.ResultsNEMO and GC scores were positively correlated with ESSG index (R¿=¿0.65, P <0.0001, and R¿=¿0.47, P <0.0001, respectively), whilst Cs score showed a negative correlation with that DA index (R¿=¿¿0.30, P <0.001). The area under the curve (AUC) of receiver operating characteristic plots, obtained by NEMO score sensitivity and specificity values in classifying patients with ESSG index ¿3.5, was significantly higher than the corresponding AUC derived from either GC or Cs scores (P <0.03 and P <0.0006, respectively). A modified score, defined by the presence of given number of MHE/MT and GC, had a good performance in classifying active patients (ESSG index ¿3, sensitivity 95.1%, specificity 84.8%, accuracy 88.7%).ConclusionsMHE/MT and GC appear to be good indicators of DA in SSc, and enhances the role of NVC as a easy technique to identify active patients.
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Low-dose oral imatinib in the treatment of systemic sclerosis interstitial lung disease unresponsive to cyclophosphamide: a phase II pilot study.
Arthritis Res. Ther.
PUBLISHED: 02-03-2014
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Pulmonary involvement represents a major cause of death of systemic sclerosis (SSc) patients. Recent data suggest that tyrosine kinase inhibitors, such as imatinib, may be a therapeutic option for SSc patients. However, preliminary published clinical trials were inconclusive about imatinib efficacy and showed side effects. The purpose of this study was to verify efficacy and tolerability of low-dose imatinib on interstitial lung disease in a cohort of SSc patients unresponsive to cyclophosphamide therapy.
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AUTOLOGOUS FAT GRAFTING IN THE TREATMENT OF FIBROTIC PERIORAL CHANGES IN PATIENTS WITH SYSTEMIC SCLEROSIS.
Cell Transplant
PUBLISHED: 10-22-2013
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Background: Autologous fat tissue grafting (AFTG) has been successfully used in the treatment of different sclerotic conditions, including localised scleroderma. Patients with advanced systemic sclerosis (SSc)-related perioral thickening and mouth opening limitation are candidates to this therapeutic approach. Aims: AFTG of the lips was performed to improve mouth opening in patients with SSc. Materials and Methods: We enrolled in the study 20 patients with diffuse SSc, (median age 35 ±15 years and 11±10 years of disease duration). Two-ml fractions of autologous fat drawn from trochanteric or peri-umbelical areas were injected in 8 different sites around the mouth. Baseline and after treatment mouth opening changes were assessed by measuring inter-incisal distance and oral perimeter, while skin hardness was tested by digital durometer. Pre- and post-treatment modifications of microvascular architecture were assessed by counting capillaries in the inferior lip videocapillaroscopy (VC) images, and by scoring the microvascular density (MVD) in anti-CD34/CD31 immuno-histochemical (IH) stained perioral skin biopsy sections. Similarly, histological sections were examined to evaluate dermo-epidermic junction (DEJ) modifications. Results: Three months after treatment, both the inter-incisal distance and oral perimeter significantly increased (p <0.001). At the same time, a significant skin neovascularization became evident, both considering the VC images (p <0.001) and MVD scores in IH sections (p <0.0001). Finally, some skin histological aspects also improved, as shown by the significant changes in DEJ flattening scores (p <0.0001). Conclusions: The present study suggests that, in patients with SSc, AFTG can improve mouth opening and function, induce a neovascularization, and partially restore the skin structure.
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Inflammation in dry eye associated with rheumatoid arthritis: cytokine and in vivo confocal microscopy study.
Innate Immun
PUBLISHED: 01-22-2013
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The purpose of this research was to study ocular surface inflammation in relation to systemic disease activity in rheumatoid arthritis (RA) patients with or without secondary Sjögrens syndrome (SSII and non-SSII respectively). The study was conducted in two phases. In phase I, 12 patients with active RA SSII and 12 with active RA non-SSII were consecutively enrolled. Each completed an Ocular Surface Disease Index (OSDI) questionnaire and underwent a full eye exam and in vivo confocal microscopy examination of the cornea. Tear fluid samples were collected in sponges and analyzed for IL-1?, -6, and -8, and TNF-?. When RA activity was suppressed by systemic treatment the patients entered phase II of the study in which all of the phase I examinations were repeated. In RA SSII patients, OSDI, fluorescein staining dendritic cell density, and concentrations of IL-1? and IL-6 decreased significantly (P?
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Antiendothelial cell antibodies induce apoptosis of bone marrow endothelial progenitors in systemic sclerosis.
J. Rheumatol.
PUBLISHED: 08-17-2010
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Patients with systemic sclerosis (SSc) have significantly fewer and functionally impaired endothelial progenitor cells (EPC) in peripheral blood and bone marrow; further, endothelial apoptosis seems to play a primary role in the pathogenesis of vascular damage. We investigated whether the failure of bone marrow EPC is related to their apoptotic phenotype and analyzed the possible mechanisms inducing apoptosis.
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Management of antiphospholipid syndrome.
Ther Adv Musculoskelet Dis
PUBLISHED: 08-01-2010
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The antiphospholipid syndrome (APS) is an autoimmune disorder presenting with tissue injury in various organs related to large- or small-vessel thrombosis associated with antiphospholipid and antiprotein/phospholipid complex antibodies. Although the pathophysiology, diagnosis, and clinical scenario may seem clear and straightforward, a more detailed examination reveals a more complex and uncertain picture related to the management of APS. This article reviews the current situation relating to APS therapy by evaluating the different clinical features of the syndrome ranging from thrombosis to pregnancy complications together with new strategies and pharmacological approaches.
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Serologic profile and mortality rates of scleroderma renal crisis in Italy.
J. Rheumatol.
PUBLISHED: 06-01-2009
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To analyze clinical and serological characteristics of subjects with scleroderma renal crisis (SRC) in Italian patients with systemic sclerosis (SSc).
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What is Visualize?

JoVE Visualize is a tool created to match the last 5 years of PubMed publications to methods in JoVE's video library.

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We use abstracts found on PubMed and match them to JoVE videos to create a list of 10 to 30 related methods videos.

Video X seems to be unrelated to Abstract Y...

In developing our video relationships, we compare around 5 million PubMed articles to our library of over 4,500 methods videos. In some cases the language used in the PubMed abstracts makes matching that content to a JoVE video difficult. In other cases, there happens not to be any content in our video library that is relevant to the topic of a given abstract. In these cases, our algorithms are trying their best to display videos with relevant content, which can sometimes result in matched videos with only a slight relation.