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Find video protocols related to scientific articles indexed in Pubmed.
Using the multi-parameter variability of photoplethysmographic signals to evaluate short-term cardiovascular regulation.
J Clin Monit Comput
PUBLISHED: 11-12-2014
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Transient changes in cardiovascular regulatory activities are valuable for clinical monitoring and pathophysiological research. However, there is a lack of effective methods to evaluate short-term cardiovascular regulation. This study explores a photoplethysmography (PPG)-based multi-parameter analytical approach using the period, amplitude and baseline of PPG signals to quantitatively assess cardiovascular regulation over a 30 s period. PPG data were recorded from 31 young healthy subjects during conditions of spontaneous respiration, paced respiration with 15 breaths/min and breath holding, with each condition lasting 30 s. The three indices (SD1, SD2, SD1/SD2) in Poincaré plot of the normalized parameters and the correlations between the parameters or the first differences of the parameters were analyzed. The results showed that compared with spontaneous respiration, SD1 of PPG amplitude increased significantly but the correlations between any two parameters or between the first differences of any two parameters decreased significantly during paced respiration; SD1 and SD1/SD2 of all PPG parameters as well as the correlations of the first differences between any two PPG parameters reduced significantly during breath holding. The results indicate the respiratory induced alterations in cardiovascular autonomic function could be identified by the variability of various PPG parameters or their correlations over 30 s periods. Moreover, the indicators used to quantify the variability of the PPG parameters in this study may provide a feasible and effective way to evaluate short-term cardiovascular regulation.
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Economical Way to Synthesize SSZ-13 with Abundant Ion-Exchanged Cu(+) for an Extraordinary Performance in Selective Catalytic Reduction (SCR) of NOx by Ammonia.
Environ. Sci. Technol.
PUBLISHED: 11-04-2014
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In this study, an economical way for SSZ-13 preparation with the essentially cheap choline chloride as template has been attempted. The as-synthesized SSZ-13 zeolite after ion exchange by copper nitrate solution exhibited a superior SCR performance (over 95% NOx conversion across a broad range from 150 to 400 °C) to the traditional zeolite-based catalysts of Cu-Beta and Cu-ZSM-5. Furthermore, the opportune size of pore opening (?3.8 Å) made Cu-SSZ-13 exhibiting the best selectivity to N2 as well as satisfactory tolerance toward SO2 and C3H6 poisonings. The characterization (XRD, XPS, XRF, and H2-TPR) of samples confirmed that Cu-SSZ-13 possessed the most abundant Cu cations among three investigated Cu-zeolites; furthermore, either on the surface or in the bulk the ratio of Cu(+)/Cu(2+) ions for Cu-SSZ-13 is also the highest. New finding was announced that CHA-type topology is in favor of the formation of copper cations, especially generating much more Cu(+) ions than the others, rather than CuO. The activity test of Cu(CuCl)-ZSM-5 (prepared by a solid-state ion-exchange method) clearly indicated that Cu(+) ions could make a major contribution to the low-temperature deNOx activity. The activity of protonic zeolites (H-SSZ-13, H-Beta, H-ZSM-5) revealed the topology effect on SCR performances.
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Design and Optimization of Novel Hydroxamate-Based Histone Deacetylase Inhibitors of Bis-Substituted Aromatic Amides Bearing Potent Activities against Tumor Growth and Metastasis.
J. Med. Chem.
PUBLISHED: 11-01-2014
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Histone deacetylases (HDACs) are one of the most promising drug targets for cancer therapy, and since more than 90% of all cancer-related deaths are associated with tumor metastasis, developing strategies to inhibit tumor metastasis while retaining anti-tumor growth activity are of great interest. Herein we demonstrated the design and identification of a series of novel hydroxamate-based HDAC inhibitors bearing potent activities against tumor growth and metastasis. Optimization of the initial hit resulted in the discovery of new HDAC inhibitors through studying the structure-activity relationship. Among them, compound 11b, one of the most potent leads, exhibited nanomolar IC50 values toward inhibition of class I and IIb HDACs as well as sub-micromolar activity against proliferation and migration of breast cancer cells in vitro. More importantly, it also significantly suppressed tumor growth in a breast tumor xenograft mouse model and dose-dependently blocked in vivo tumor metastasis in a mouse pulmonary metastasis model.
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Metal non-oxide nanostructures developed from organic-inorganic hybrids and their catalytic application.
Nanoscale
PUBLISHED: 10-24-2014
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The rational design of metal non-oxides is important for their catalytic application, which is however limited by the fact that the current synthetic strategies are short of effective control over formation reactions. Recently, the hybrids evenly integrating organic with inorganic molecules on a nanoscale significantly provided quasi-homogeneous reactions towards well-defined nanocatalysts of metal non-oxides, in which their structures and properties can be modulated in a wide range. Focusing on the nanostructures and the related catalytic behaviors, this feature article seeks to provide some control on the key structures and properties of metal non-oxides (e.g. carbides, nitrides, sulfides and selenides). It is thus anticipated to shed some light on the development of emerging materials for efficient catalysis, especially those used in energy utilization.
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Photoluminescence properties of hybrid SiO2-coated CdTe/CdSe quantum dots.
Luminescence
PUBLISHED: 10-23-2014
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Hybrid SiO2-coated CdTe/CdSe quantum dots (QDs) were prepared using CdTe/CdSe QDs prepared by hydrothermal synthesis. A CdSe interlayer made CdTe/CdSe cores with unique type II heterostructures. The hybrid SiO2-coated CdTe/CdSe QDs revealed excellent photoluminescence (PL) properties compared with hybrid SiO2-coated CdTe QDs. Because of the existence of spatial separations of carriers in the type II CdTe/CdSe core/shell QDs, the hybrid QDs had a relatively extended PL lifetime and high stability in phosphate-buffered saline buffer solutions. This is ascribed to the unique components and stable surface state of hybrid SiO2-coated CdTe/CdSe QDs. During the stabilization test in phosphate-buffered saline buffer solutions, both static and dynamic quenching occurred. The quenching mechanism of the hybrid QDs was not suited with the Stern–Volmer equation. However, the relative stable surface of CdTe/CdSe QDs resulted in lower degradation and relative high PL quantum yields compared with hybrid SiO2-coated CdTe QDs. As a result, hybrid SiO2-coated CdTe/CdSe QDs can be used in bioapplications.
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Randomized Controlled Trial of Early Rehabilitation After Intracerebral Hemorrhage Stroke: Difference in Outcomes Within 6 Months of Stroke.
Stroke
PUBLISHED: 10-23-2014
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Mechanisms, acute management, and outcomes for patients who experience intracerebral hemorrhage may differ from patients with ischemic stroke. Studies of very early rehabilitation have been mainly undertaken in patients with ischemic stroke, and it is unknown if benefits apply to those with intracerebral hemorrhage. We hypothesized that early rehabilitation, within 48 hours of stroke, would improve survival and functional outcomes in patients with intracerebral hemorrhage.
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Diagnostic value of surfactant protein-a in severe acute pancreatitis-induced acute respiratory distress syndrome.
Med. Sci. Monit.
PUBLISHED: 09-27-2014
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The complexity of multiple-item criteria in acute respiratory distress syndrome (ARDS) often causes inconvenience for physicians in the management of patients with severe acute pancreatitis (SAP). We evaluated whether serum SP-A levels in the presence of diffuse alveolar damage (DAD) can be qualitatively assessed for diagnosis of SAP-induced ARDS.
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MicroRNA-27a promotes proliferation and suppresses apoptosis by targeting PLK2 in laryngeal carcinoma.
BMC Cancer
PUBLISHED: 09-17-2014
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miRNA-27a has been confirmed as an important regulator in carcinogenesis and other pathological processes. Whether and how it plays a role in the laryngeal carcinoma is unknown.
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Standard b-value versus low b-value diffusion-weighted MRI in renal cell carcinoma: a systematic review and meta-analysis.
BMC Cancer
PUBLISHED: 09-16-2014
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We sought to determine the comparative diagnostic performance of standard b-value (800-1000 s/mm2) versus low b-value (400-500 s/mm2) diffusion-weighted magnetic resonance imaging (DW-MRI) in the detection of renal cell carcinoma (RCC).
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[Mutation analysis of mucopolysaccharidosis type II and prenatal diagnosis].
Zhonghua Fu Chan Ke Za Zhi
PUBLISHED: 08-30-2014
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To analyze the mutations of IDS gene in a mucopolysaccharidosis type II (MPSII) family and to make prenatal diagnosis on the high-risk fetus which has been pregnant for eleven weeks.
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Anti-oxidative nutrient rich diet protects against acute ischemic brain damage in rats.
Brain Res.
PUBLISHED: 08-29-2014
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We evaluated the neuroprotective effects of an anti-oxidative nutrient rich enteral diet (AO diet) that contained rich polyphenols (catechins and proanthocyanidins) and many other anti-oxidative ingredients. Wistar rats were treated with either vehicle, normal AO diet (containing 100kcal/100mL, catechin 38.75mg/100mL and proanthocyanidin 19mg/100mL, 1mL/day), or high AO diet (containing 10 times the polyphenols of the normal AO diet) for 14 days, and were subjected to 90min of transient middle cerebral artery occlusion. The AO diet improved motor function, reduced cerebral infarction volume, and decreased both peroxidative markers such as 4-hydroxynonenal, advanced glycation end products, 8-hydroxy-2-deoxyguanosine and inflammatory markers such as monocyte chemotactic protein-1, ionized calcium-binding adapter molecule-1, and tumor necrosis factor-?. Our study has shown that an AO diet has neuroprotective effects through both anti-oxidative and anti-inflammatory mechanisms, indicating that nutritional control with polyphenols could be useful for patients with acute ischemic stroke.
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[Clinical characteristics of adrenal gland trauma].
Zhonghua Yi Xue Za Zhi
PUBLISHED: 08-26-2014
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To explore the clinical characteristics of adrenal gland trauma.
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Hydrogenated Anatase: Strong Photocatalytic Dihydrogen Evolution without the Use of a Co-Catalyst.
Angew. Chem. Int. Ed. Engl.
PUBLISHED: 08-24-2014
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The high-pressure hydrogenation of commercially available anatase or anatase/rutile TiO2 powder can create a photocatalyst for H2 evolution that is highly effective and stable without the need for any additional co-catalyst. This activation effect cannot be observed for rutile; however, for anatase/rutile mixtures, a strong synergistic effect can be found (similar to results commonly observed for noble-metal-decorated TiO2 ). EPR and PL measurements indicated the intrinsic co-catalytic activation of anatase TiO2 to be due to specific defect centers formed during hydrogenation. These active centers can be observed specifically for high-pressure hydrogenation; other common reduction treatments do not result in this effect.
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[Mutation analysis and prenatal diagnosis in families of X-linked agammaglobulinemia caused by BTK gene mutation].
Zhonghua Yi Xue Za Zhi
PUBLISHED: 08-22-2014
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To evaluate the genetic diagnostic feasibility of Bruton's tyrosine kinase (BTK) gene in three families with X-linked agammagobulinemia (XLA) birth history, mutation analysis and prenatal genetic diagnosis of BTK gene for two families with XLA.
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Exploring the rising incidence of neuroendocrine tumors: A population-based analysis of epidemiology, metastatic presentation, and outcomes.
Cancer
PUBLISHED: 08-19-2014
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An increased incidence of neuroendocrine tumors (NETs) has been reported worldwide, but the reasons underlying this rise have not been identified. By assessing patterns of metastatic presentation, this study sought to examine the epidemiologic characteristics of NETs and the contribution of early-stage detection to the rising incidence.
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Aloperine attenuated neuropathic pain induced by chronic constriction injury via anti-oxidation activity and suppression of the nuclear factor kappa B pathway.
Biochem. Biophys. Res. Commun.
PUBLISHED: 08-12-2014
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To investigate whether aloperine (ALO) has antinociceptive effects on neuropathic pain induced by chronic constriction injury, whether ALO reduces ROS against neuropathic pain, and what are the mechanisms involved in ALO attenuated neuropathic pain.
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KTP crystal thickness distribution measurements based on laser feedback interferometry.
Appl Opt
PUBLISHED: 08-05-2014
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KTiOPO(4) (KTP) crystal is a widely used nonlinear optical crystal, and it can meet high requirements for parallelism of crystal surfaces and the length in the light propagation direction in its application fields. In this paper, we present a method for measuring the thickness distribution of cuboid KTP crystals based on laser feedback interferometry (LFI). The accuracy of the measurements for the relative thickness distribution was 8.8 nm, and that of the absolute thickness can be improved by increasing the accuracy of the refractive index. This method is applicable to measurements of all light transmissive birefringent materials, and the results provide detailed instructions for crystal processing and polishing.
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Nomenclatural changes, new country records and range extensions of Baridinae (Coleoptera, Curculionidae) from China.
Zootaxa
PUBLISHED: 07-25-2014
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Thirteen relatively frequently collected species of baridine weevils from mainland China were mapped to improve our understanding of biogeographic patterns and distributional ranges of Baridinae in the Palaearctic and Oriental regions. This simple case study showed that taxonomists tend to underestimate the spatial scope required in regional investigations and descriptive works often resulting in widespread species being described numerous times. Several apparently uncommon species with uncertain relationships, known previously only from moderately high latitudes in the Palaearctic region, showed Oriental affinities. In each region, species may occur over many thousands of kilometers not only in East-West but also in North-South directions. New synonyms are Mimophilus Faust (=Baridiomorphus Voss), Acythopeus inflatirostris Voss (=Acythopeus proximus Voss), Baris albisquama Zaslavskij (=Baris ljaodunensis Zaslavskij), Mimophilus tragicus Faust (=Baridiomorphus conicollis Voss), Moreobaris deplanata (Roelofs) (=Acythopeus patruelis Voss), Baris pilosa Roelofs (=Baris blennus Marshall, =Baris piliventris Zaslavskij, =Baris pygidialis Voss, =Baris suvorovi Reitter), Baris artemisiae (Panzer) (=Baris corvina Voss), Pellobaris melancholica (Roelofs) (=Paracythopeus collaris Voss), Pteridobaris maritima (Roelofs) (=Baris quinquecarinata Zaslavskij) and Ulobaris kuchenbeisseri Hartmann (=Baris pseudospitzyi Zaslavskij, =Baris ussuriensis ussuriensis Zaslavskij, =Baris ussuriensis chinganensis Zaslavskij). New combinations are Athesapeuta gracilis (Voss) (from Eumycterus Schönherr), Athesapeuta inornata (Voss) (from Baris Germar) and Nespilobaris inflatirostris (Voss) (from Acythopeus Pascoe). Baridius vestitus Perris (not Boheman) and Baris pygidialis Hustache (not Voss) are newly recognized primary homonyms. A lectotype is designated for Moreobaris deplanata. Twenty-four species are recorded newly from China. 
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Cloning and characterization of a new ?-Glucosidase from a metagenomic library of Rumen of cattle feeding with Miscanthus sinensis.
BMC Biotechnol.
PUBLISHED: 07-09-2014
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The study on the second generation bio-fuel is a hot area of current research of renewable energy. Among series of key points in this area, the role of ?-glucosidase in the degradation of intermediate gluco-oligosaccharides limits the rate of the complete saccharification of lignocellulose.
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A computational study on the complexation of Np(V) with N,N,N',N'-tetramethyl-3-oxa-glutaramide (TMOGA) and its carboxylate analogs.
Phys Chem Chem Phys
PUBLISHED: 07-03-2014
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Density functional theory has been used to study the geometries and relative stabilities of the complexes of NpO2(+) with the title compounds (L), including TMOGA, deprotonated N,N'-dimethyl-3-oxa-glutaramic acid (DMOGA) and their deprotonated oxydiacetic analog (ODA). Our calculations suggest that the complexes where the ligands appear as tridentate chelators are more stable than as bidentate ones, and the substitution of the amide group by carboxylate favors the formation of the complexes. Thermodynamically the 1?:?2 complex (Np-L2) is more favorable than the 1?:?1 complex (Np-L) in the cases of TMOGA and DMOGA, but not for the ODA anion. Taking into account the solvation effect of water, the 1?:?2 complex is more favorable than the 1?:?1 complex for all of the three ligands, though the reaction enthalpy decreases compared to that in the gas phase, and the formation of Np-(TMOGA)2 from Np-TMOGA is roughly a thermal neutral process. The strength of the Np=O bond is weakened upon the coordination of ligands to Np(V) and the increase of the negative charge on the ligand (-1e for deprotonated DMOGA and -2e for deprotonated ODA). The Quantum Theory of Atoms-in-Molecules (QTAIM) was used here to analyze the bonding mode of NpO2(+)-Lx (x = 1, 2) and to compare the bond order data.
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MiR-143 acts as a tumor suppressor by targeting N-RAS and enhances temozolomide-induced apoptosis in glioma.
Oncotarget
PUBLISHED: 07-02-2014
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Therapeutic applications of microRNAs (miRNAs) in RAS-driven glioma were valuable, but their specific roles and functions have yet to be fully elucidated. Here, we firstly report that miR-143 directly targets the neuroblastoma RAS viral oncogene homolog (N-RAS) and functions as a tumor-suppressor in glioma. Overexpression of miR-143 decreased the expression of N-RAS, inhibited PI3K/AKT, MAPK/ERK signaling, and attenuated the accumulation of p65 in nucleus of glioma cells. In human clinical specimens, miR-143 was downregulated where an adverse with N-RAS expression was observed. Furthermore, overexpression of miR-143 decreased glioma cell migration, invasion, tube formation and slowed tumor growth and angiogenesis in a manner associated with N-RAS downregulation in vitro and in vivo. Finally, miR-143 also sensitizes glioma cells to temozolomide (TMZ),the first-line drug for glioma treatment. Taken together, for the first time, our results demonstrate that miR-143 plays a significant role in inactivating the RAS signaling pathway through the inhibition of N-RAS, which may provide a novel therapeutic strategy for treatment of glioma and other RAS-driven cancers.
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End-of-life home care utilization and costs in patients with advanced colorectal cancer.
J Community Support Oncol
PUBLISHED: 06-28-2014
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To determine overall utilization and costs associated with home care services in Ontario, Canada by linking a home care database to a stage IV colorectal cancer cohort.
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Ammonia drives dendritic cells into dysfunction.
J. Immunol.
PUBLISHED: 06-25-2014
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Ammonia levels are often elevated in patients with cirrhosis or tumors. Patients with these diseases are immunocompromised. In this study, we investigated the effects of ammonia on a member of the immune cell family, the dendritic cells (DCs). Our results demonstrated that ammonia diminished cell count, phagocytosis, and lymphocyte stimulation of DCs. Ammonia also induced DC swelling, excessive reactive oxygen species production, and mitochondrial damage, which may constitute the underlying mechanism of ammonia-induced DC dysfunction. In ammonium chloride (NH4Cl)-loaded mice, DCs exhibited lowered phagocytosis and a weakened immune response to the chicken OVA vaccine. DCs from patients with cirrhosis or ammonia-treated healthy human blood both exhibited diminished phagocytosis. Moreover, tumor cell conditioned medium drove DCs into dysfunction, which could be reversed by ammonia elimination. In a murine colon carcinoma model, we found that ammonia could regulate tumor growth involving DCs and their related immune response. These findings reveal that ammonia could drive DCs into dysfunction, which contributes to the immunocompromised state of patients with cirrhosis or tumors.
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[Clinical study of tissue-selecting therapy in the treatment of mixed hemorrhoids: a single-blind randomized controlled trail].
Zhonghua Wei Chang Wai Ke Za Zhi
PUBLISHED: 06-24-2014
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To evaluate the clinical efficacy and safety of tissue-selecting therapy (TST) in treatment of mixed hemorrhoids.
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MicroRNA-377 inhibited proliferation and invasion of human glioblastoma cells by directly targeting specificity protein 1.
Neuro-oncology
PUBLISHED: 06-20-2014
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Increasing evidence has indicated that microRNAs (miRNAs) are strongly implicated in the initiation and progression of glioblastoma multiforme (GBM). Here, we identi?ed a novel tumor suppressive miRNA, miR-377, and investigated its role and therapeutic effect for GBM.
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[Expression of peroxisome proliferators-activated receptor in glioma and its effect on the growth of human glioma cells].
Zhonghua Yi Xue Yi Chuan Xue Za Zhi
PUBLISHED: 06-15-2014
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To study the expression of peroxisome proliferators-activated receptor (PPAR) in human glioma tissue and its influence on tumor growth.
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[Mutation analyses and prenatal diagnosis in two families of X linked severe combined immunodeficiency caused by IL2RG gene novel mutation].
Zhonghua Yi Xue Za Zhi
PUBLISHED: 06-14-2014
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To evaluate the diagnostic feasibility of mutation analysis and prenatal genetic diagnosis genetic analysis of IL2RG gene in two families with a birth history of X-linked severe combined immunodeficiency (X-SCID).
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Transplanted hUCB-MSCs migrated to the damaged area by SDF-1/CXCR4 signaling to promote functional recovery after traumatic brain injury in rats.
Neurol. Res.
PUBLISHED: 06-12-2014
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Transplanted human umbilical cord mesenchymal stem cells (hUC-MSCs) have exhibited considerable therapeutic potential for traumatic brain injury (TBI). However, how hUC-MSCs migrating to the injury region and the mechanism of hUC-MSCs promoting functional recovery after TBI are still unclear. In this study, we investigated whether stromal cell-derived factor-1 (SDF-1) was involved in the hUC-MSCs migration and the possible mechanisms that might be involved in the beneficial effect on functional recovery. In vitro experiments demonstrated that SDF-1 induces a concentration-dependent migration of hUC-MSCs. Furthermore, pre-treatment with the CXCR4-specific antagonist AMD3100 significantly prevented the migration of hUC-MSCs in vitro. We found that the expression of SDF-1 increased significantly around the damaged area. Transplanted hUC-MSCs were localized to regions where SDF-1 was highly expressed. Additionally, our results showed that hUC-MSCs-treated animals showed significantly improved functional recovery compared with controls. In hUC-MSCs-transplanted group, terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling (TUNEL)-positive cells were decreased and BrdU-positive cells were significantly increased compared with control group, more of BrdU-positive cells co-localized with GFAP. These suggest that SDF-1 plays an important role in the migration of hUC-MSCs to the damaged area and hUC-MSCs are beneficial for functional recovery after TBI.
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NH? treatment of TiO? nanotubes: from N-doping to semimetallic conductivity.
Chem. Commun. (Camb.)
PUBLISHED: 06-12-2014
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In the present work we show that a suitable high temperature ammonia treatment allows for the conversion of single-walled TiO2 nanotube arrays not only to a N-doped photoactive anatase material (which is already well established), but even further into fully functional titanium nitride (TiN) tubular structures that exhibit semimetallic conductivity.
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Inhibition of activated Ras suppresses multiple oncogenic Hub genes in human epithelial tumors.
Int. J. Oncol.
PUBLISHED: 06-10-2014
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Cancer cells may involve diverse mutations, but they often rely on continued expression of a single oncoprotein for survival, as a response to targeting this protein. Generally, Ras is overexpressed in human epithelial tumors and cancellation of activated Ras inhibits carcinoma cell proliferation and differentiation ability, and induces apoptotosis of tumor cells. However, the mechanisms of inhibition of activated Ras that suppress the malignancy activity of human epithelial tumors remain to be illuminated. We utilized text-mining of MEDLINE abstracts with natural language processing to establish the Ras biologic association network, and identified several interactions of this network with the Ras pathway. Our investigation not only examined the expression of Ras and Hub genes (PIK3CA, MDM2, CCND1, EGFR, JUN, MYC, VEGFA, ERK1 and ERK2) but also confirmed inhibition of activated Ras reduced expression of multiple oncogene in vitro studies. Our studies provide strong support for the conclusion that cancellation of activated Ras specifically regulates defective Ras pathways in human tumor cells.
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Association between metabolic syndrome and osteoporotic fracture in middle-aged and elderly Chinese peoples.
Cell Biochem. Biophys.
PUBLISHED: 06-09-2014
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Associations between metabolic syndrome (MetS) and osteoporotic fracture have been reported. However, the epidemiological studies are not conclusive. The objective of the study was to determine whether MetS associates with osteoporotic fracture. This was a cross-sectional study of 9,930 Chinese adults aged 40 years or older in the Chongming District, Shanghai, China. A questionnaire, anthropometric measurements and laboratory tests were conducted. Metabolic syndrome was defined according to the updated National Cholesterol Education Program Adult Treatment Panel III criteria for Asian Americans. A history of fractures was collected with an interviewer-assisted questionnaire. Osteoporotic fractures were defined as fractures that occurred due to low trauma in 2 years prior to the study. Among women, the prevalence of osteoporotic fractures was significantly higher in those with MetS (3.5 vs. 2.6 %, = 0.028). However, the difference was not found in men (2.6 vs. 2.4 %, P = 0.737). The presence of MetS was significantly associated with increased odds of osteoporotic fracture among women (odds ratio 1.22; 95 % confidence interval 1.12-1.54; P = 0.039) after controlling for potential confounders. The significant associations were not detected in men. The presence of MetS was significantly associated with a recent history of osteoporotic fracture in middle-aged and elderly Chinese women.
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Decompression alone versus decompression with instrumented fusion for young patients with single-level lumbar disc herniation: a short-term prospective comparative study.
Chin. Med. J.
PUBLISHED: 06-04-2014
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For young patients, the surgical method for lumbar disc herniation remains controversial. The aim of this study was to prospectively determine the short-term clinical outcome after surgery for young patients with lumbar disc herniation.
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A brightness-referenced star identification algorithm for APS star trackers.
Sensors (Basel)
PUBLISHED: 05-22-2014
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Star trackers are currently the most accurate spacecraft attitude sensors. As a result, they are widely used in remote sensing satellites. Since traditional charge-coupled device (CCD)-based star trackers have a limited sensitivity range and dynamic range, the matching process for a star tracker is typically not very sensitive to star brightness. For active pixel sensor (APS) star trackers, the intensity of an imaged star is valuable information that can be used in star identification process. In this paper an improved brightness referenced star identification algorithm is presented. This algorithm utilizes the k-vector search theory and adds imaged stars' intensities to narrow the search scope and therefore increase the efficiency of the matching process. Based on different imaging conditions (slew, bright bodies, etc.) the developed matching algorithm operates in one of two identification modes: a three-star mode, and a four-star mode. If the reference bright stars (the stars brighter than three magnitude) show up, the algorithm runs the three-star mode and efficiency is further improved. The proposed method was compared with other two distinctive methods the pyramid and geometric voting methods. All three methods were tested with simulation data and actual in orbit data from the APS star tracker of ZY-3. Using a catalog composed of 1500 stars, the results show that without false stars the efficiency of this new method is 4~5 times that of the pyramid method and 35~37 times that of the geometric method.
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Diagnosis and treatment of pancreatic metastases in 22 patients: a retrospective study.
World J Surg Oncol
PUBLISHED: 05-16-2014
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Pancreatic metastases (PMs) are rare and lack of guidelines for diagnosis and treatments .The aim of this study is to explore the diagnosis, treatment, and prognosis of pancreatic metastases.
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Rapid detection and characterization of major phenolic compounds in Radix Actinidia chinensis Planch by ultra-performance liquid chromatography tandem mass spectrometry.
J Pharm Biomed Anal
PUBLISHED: 05-12-2014
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Radix Actinidia chinensis Planch is a traditional Chinese herb, and its decotion had been widely used clinically to treat several types of cancer. In our study, the phenolic compounds constituting the major water soluble components of Radix A. chinensis were profiled and characterized by ultraperformance liquid chromatography electrospray ionization tandem mass spectrometry. A total of 50 compounds were identified or tentatively characterized, including caffeic acid, p-coumaric acid, 29 catechin derivatives, 12 quinic acid derivatives and 7 coumarin derivatives. Most of the identified compounds were firstly reported from A. chinensis. Among them, scopoletin, scoplin, isofraxoside and quinic acid derivatives have not ever been reported from genus Actinidia previously. These phenolic compounds might be responsible for the antitumor activity of the water extract of radix A. chinensis, and the established analytical method could be applied to further study of quality evaluation and active components of Radix A. chinensis.
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miR-622 suppresses proliferation, invasion and migration by directly targeting activating transcription factor 2 in glioma cells.
J. Neurooncol.
PUBLISHED: 04-06-2014
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Malignant gliomas are the most common and devastating primary brain tumors in adults. The rapid invasion of tumor cells into the adjacent normal brain tissues is a major cause of treatment failure, yet the mechanisms that regulate this process remain poorly understood. MicroRNAs have recently emerged as regulators of invasion and metastasis by acting on multiple signaling pathways. In this study, we found that miR-622 is significantly downregulated in glioma tissues and cell lines. Functional experiments showed that increased miR-622 expression reduced glioma cell invasion and migration, whereas decreased miR-622 expression enhanced cell invasion and migration. Moreover, activating transcription factor 2 (ATF2), an important transcription factor that regulate tumor invasion, was identified as a direct target of miR-622. Knockdown of ATF2 using small interefering RNA recapitulated the anti-invasive function of miR-622, whereas restoring the ATF2 expression attenuated the function of miR-622 in glioma cells. Furthermore, clinical data indicated that miR-622 and ATF2 were inversely expressed in glioma specimens. Our findings provide insight into the specific biological behavior of miR-622 in tumor invasion and migration. Targeting miR-622/ATF2 axis is a novel therapeutic approach for blocking glioma invasion.
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Hexabromocyclododecanes in surface sediments from Shanghai, China: spatial distribution, seasonal variation and diastereoisomer-specific profiles.
Chemosphere
PUBLISHED: 04-02-2014
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The concentrations, spatial distribution, diastereoisomer-specific profiles of hexabromocyclododecanes (HBCDs) in surface sediments from three major rivers in Shanghai as well as the seasonal variation of HBCDs were investigated. The concentrations of total HBCD diastereoisomers ranged from 0.05 to 6.87ngg(-1)dw. Significant spatial distribution of total HBCDs concentrations were observed. The mean concentration of total HBCDs followed the order of Suzhou Creek (2.00ngg(-1))>Huangpu River (1.59ngg(-1))>Yunzao Creek (0.77ngg(-1)). The concentration was a relatively low HBCDs level compared to levels measured in domestic and other parts of the world. The proportions of ?-HBCD in sediment samples were generally higher than that of commercial formulations. This might be due to thermal isomerization from ?-HBCD to ?-HBCD and slower degradation rate of ?-HBCD compared to ?-HBCD in anaerobic conditions. The concentrations of ?HBCD in the summer were significantly higher than those in the winter (paired t test, p<0.01). This seasonal variation could probably be attributed to a combined effect of temperature and wet deposition. Moreover, a poor and no significant correlation between ?HBCD levels and TOC content in sediments was observed, suggesting that the spatial distributions of HBCDs were not constrained by the TOC in sediments of Shanghai.
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Decreased serum levels of free fatty acids are associated with breast cancer.
Clin. Chim. Acta
PUBLISHED: 03-23-2014
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Changes in the levels of lipids are associated with breast cancer (BC).
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Prognostic nutritional index predicts postoperative complications and long-term outcomes of gastric cancer.
World J. Gastroenterol.
PUBLISHED: 03-12-2014
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To investigate the impact of prognostic nutritional index (PNI) on the postoperative complications and long-term outcomes in gastric cancer patients undergoing total gastrectomy.
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Three puncture sites used for in utero electroporation show no significantly different negative impacts during gene transfer into the embryonic mouse brain.
Neurosci. Lett.
PUBLISHED: 03-09-2014
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Although various ways to manipulate genes in vivo exist, in utero electroporation is a widely used technique, especially in the field of neural development due to its many advantages. In this study, we focused on direct comparison between three puncture sites during in utero electroporation on the death rate of embryos, the thickness and the area of cortex, cell differentiation, cell proliferation, cell migration and cell apoptosis. We found no statistical significant differences between the three puncture methods in the death rate of embryos, the thickness and the area of cortex, cell differentiation, cell proliferation, cell migration and cell apoptosis.
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Complete sequence of a novel duck astrovirus.
Arch. Virol.
PUBLISHED: 02-28-2014
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A duck astrovirus isolate CPH (DAstV/CPH) detected from newly hatched ducklings was sequenced. The 7463-nt-long genome contained three open reading frames (ORFs) that were in three different frames, as seen in DAstV/C-NGB. Sequence comparison and phylogenetic analysis based on the full-length amino acid sequences of the three ORFs demonstrated that DAstV/CPH was highly divergent from previously known avastroviruses for which corresponding sequences are available. Genetic analysis of the complete ORF2 region revealed that mean amino acid genetic distances shared by DAstV/CPH with members of the three established avastrovirus species and other avastroviruses (except the chicken astrovirus GA2011 [CAstV/GA2011]-like viruses) were between 0.577 and 0.787, suggesting that DAstV/CPH may represent an additional avastrovirus species. The mean amino acid genetic distance between DAstV/CPH and the CAstV/GA2011-like viruses was 0.366, demonstrating the difficulty in determining the taxonomic relationship of DAstV/CPH to the CAstV/GA2011-like viruses. Interestingly, analysis based on a portion of the ORF1b region indicated that DAstV/CPH was more closely related to the common-teal-origin astrovirus MPK514 than to the CAstV/GA2011-like viruses. The findings are likely to provide new insights into the ecology and evolution of avastroviruses.
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Dehydration stress memory genes of Zea mays; comparison with Arabidopsis thaliana.
BMC Plant Biol.
PUBLISHED: 02-27-2014
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Pre-exposing plants to diverse abiotic stresses may alter their physiological and transcriptional responses to a subsequent stress, suggesting a form of "stress memory". Arabidopsis thaliana plants that have experienced multiple exposures to dehydration stress display transcriptional behavior suggesting "memory" from an earlier stress. Genes that respond to a first stress by up-regulating or down-regulating their transcription but in a subsequent stress provide a significantly different response define the 'memory genes' category. Genes responding similarly to each stress form the 'non-memory' category. It is unknown whether such memory responses exists in other Angiosperm lineages and whether memory is an evolutionarily conserved response to repeated dehydration stresses.
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Node-extranodal soft tissue stage based on extranodal metastasis is associated with poor prognosis of patients with gastric cancer.
J. Surg. Res.
PUBLISHED: 02-21-2014
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The purpose of this study was to evaluate the effect of Node-Extranodal soft tissue (pNE) stage based on Extranodal Metastasis (EM) on recurrence and survival in patients with gastric cancer (GC).
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HJB-1, a 17-hydroxy-jolkinolide B derivative, inhibits LPS-induced inflammation in mouse peritoneal macrophages.
Int. Immunopharmacol.
PUBLISHED: 02-16-2014
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Jolkinolide B (JB) and 17-hydroxy-JB (HJB) are diterpenoids from plants and it has been reported that the presence of a C-17 hydroxy group in JB significantly enhances the anti-inflammatory potency of JB. In this study, two HJB derivatives HJB-1 and HJB-2 were generated by the chemical modification of a 17-hydroxy group of HJB. HJB-1 more effectively inhibited TNF-?, IL-1? and IL-6 release in LPS-stimulated mouse peritoneal macrophages. In addition, HJB-1 reduced LPS-induced mRNA expression of TNF-?, IL-1?, IL-6, COX-2 and iNOS in a concentration-dependent manner, but did not alter IL-10 mRNA expression. LPS-induced NF-?B activation and MAPK phosphorylation were also effectively inhibited by HJB-1. These results demonstrate that HJB-1 exerts anti-inflammatory effects on LPS-activated mouse peritoneal macrophages by inhibiting NF-?B activation and MAPK phosphorylation and modification of a 17-hydroxy group of HJB may enhance the anti-inflammatory potency of HJB derivatives.
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Screening of human chromosome 21 genes in the dorsolateral prefrontal cortex of individuals with Down syndrome.
Mol Med Rep
PUBLISHED: 02-13-2014
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The aim of the current study was to identify the genes on human chromosome 21 (HC21) that may serve important functions in the pathogenesis of Down syndrome (DS). The microarray data GSE5390 were obtained from the Gene Expression Omnibus database, which contained 7 DS and 8 healthy normal samples. The data were then normalized and the differentially expressed genes (DEGs) were identified using the LIMMA package and Bonferroni correction. Furthermore, the DEGs underwent clustering and gene ontology analysis. Additionally, the locations of the DEGs on HC21 were confirmed using human genome 19 in the University of California, Santa Cruz Interaction Browser. A total of 25 upregulated and 275 downregulated genes were screened between DS and healthy samples with a false discovery rate of <0.05 and |logFC|>1. The expression levels of these genes in the two samples were different. In addition, the up? and downregulated genes were markedly enriched in organic substance biological processes (P=4.48x10?10) and cell?cell signaling (P=0.000227). Furthermore, 17 overexpressed genes were identified on the 21q21?22 area, including COL6A2, TTC3 and ABCG1. Together, these observations suggest that 17 upregulated genes on HC21 may be involved in the development of DS and provide the basis for understanding this disability.
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Pericyte protection by edaravone after tissue plasminogen activator treatment in rat cerebral ischemia.
J. Neurosci. Res.
PUBLISHED: 02-01-2014
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Pericytes play a pivotal role in contraction, mediating inflammation and regulation of blood flow in the brain. In this study, changes of pericytes in the neurovascular unit (NVU) were examined in relation to the effects of exogenous tissue plasminogen activator (tPA) and a free radical scavenger, edaravone. Immunohistochemistry and Western blot analyses showed that the overlap between platelet-derived growth factor receptor ?-positive pericytes and N-acetylglucosamine oligomers (NAGO)-positive endothelial cells increased significantly at 4 days after 90 min of transient middle cerebral artery occlusion (tMCAO). The number of pericytes and the overlap with NAGO decreased with tPA but recovered with edaravone 4 days after tMCAO with proliferation. Thus, tPA treatment damaged pericytes, resulting in the detachment from astrocytes and a decrease in glial cell line-derived neurotrophic factor secretion. However, treatment with edaravone greatly improved tPA-induced damage to pericytes. The present study demonstrates that exogenous tPA strongly damages pericytes and destroys the integrity of the NVU, but edaravone treatment can greatly ameliorate such damage after acute cerebral ischemia in rats.
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Effects of BD1047, a ?1 receptor antagonist, on the expression of mTOR, Camk2? and GSK-3? in fluvoxamine-treated N2a cells.
Exp Ther Med
PUBLISHED: 01-08-2014
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Fluvoxamine, a common antidepressant agent, is designed to exert its pharmacological effect by inhibiting synaptic serotonin reuptake. However, increasing evidence has demonstrated that ?1 receptors are likely to be involved in the mechanism of action of fluvoxamine. The present study aimed to observe the effects of fluvoxamine on the expression levels of mammalian target of rapamycin (mTOR), Ca(2+)/calmodulin-dependent protein kinase 2? (Camk2?) and glycogen synthase kinase-3? (GSK-3?) in fluvoxamine-treated N2a cells and attempted to elucidate whether ?1 receptors mediate the pharmacological effects of fluvoxamine. The N2a cells were randomly divided into three groups (each n=6): DMEM group (D group), 0.5 ?mol/l fluvoxamine group (F group) and 0.2 ?mol/l BD1047 (a ?1 receptor antagonist) + 0.5 ?mol/l fluvoxamine group (BF group). Western blotting was used to determine the expression levels of mTOR, Camk2? and GSK-3? in the cultured N2a cells after two days of incubation. The F group exhibited significant increases in the expression levels of mTOR and Camk2? and a significant reduction in the expression levels of GSK-3? compared with those in the D group (P<0.01). By contrast, the BF group demonstrated significant reductions in the expression levels of mTOR and Camk2? and a significant increase in the expression levels of GSK-3?, compared with those in the F group (P<0.01). These results suggest that ?1 receptors mediate fluvoxamine-elicited changes in the expression levels of mTOR, Camk2? and GSK-3? in N2a cells, which indicates that ?1 receptors are likely to be involved in the pharmacological effects of fluvoxamine.
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MicroRNA-223-3p Inhibits the Angiogenesis of Ischemic Cardiac Microvascular Endothelial Cells via Affecting RPS6KB1/hif-1a Signal Pathway.
PLoS ONE
PUBLISHED: 01-01-2014
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MicroRNAs (miRNAs) are a recently discovered class of posttranscriptional regulators of gene expression with critical functions in the angiogenesis and cardiovascular diseases; however, the details of miRNAs regulating mechanism of angiogenesis of ischemic cardiac microvascular endothelial cells (CMECs) are not yet reported.
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Utilization and costs of home care for patients with colorectal cancer: a population-based study.
CMAJ Open
PUBLISHED: 01-01-2014
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The utilization and costs of home care services provided for people with colorectal cancer is not well-known. We conducted an analysis to determine the utilization and costs of such services associated with each stage of colorectal cancer among patients in the province of Ontario.
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A taxonomic revision of Limnobaris Bedel in the strict sense (Coleoptera, Curculionidae, Baridinae), with particular emphasis on the species found in China.
Zookeys
PUBLISHED: 01-01-2014
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The genus name Limnobaris Bedel is applied in a restricted sense to baridine weevils with a covered pygidium and non-prominent, decussate mandibles which occur on sedges in the Palaearctic Region and immediately adjacent parts of tropical Southeast Asia. Calyptopygus Marshall and Pertorcus Voss are syn. n. of Limnobaris. Some species from Africa and the Americas are maintained provisionally in Limnobaris in the widest sense but will need to be transferred to other genera in future studies. A total of eleven species is recognized in Asia, two of which are widespread and occur also in the Western Palaearctic Region. Limnobaris martensi Korotyaev sp. n. is described from Nepal. Pertorcus tibialis basalis Voss is raised to species rank, as L. basalis (stat. prom.). New or reestablished synonyms are L. dolorosa (Goeze) (= L. jucunda Reitter, = L. koltzei Reitter), L. tibialis (Voss) (= Pertorcus tibialis pilifer Voss) and L. t-album (Linnaeus) (= L. bedeli Reitter, = Baridius crocopelmus Gyllenhal, = L. sahlbergi Reitter, = L. scutellaris Reitter, = Baris t-album sculpturata Faust). Calandra uniseriata Dufour is considered a junior synonym of Sitophilus oryzae (L.) (syn. n.). A key for identification and a distribution map are provided.
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An axis involving SNAI1, microRNA-128 and SP1 modulates glioma progression.
PLoS ONE
PUBLISHED: 01-01-2014
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Glioblastoma is an extraordinarily aggressive disease that requires more effective therapeutic options. Snail family zinc finger 1, dysregulated in many neoplasms, has been reported to be involved in gliomas. However, the biological mechanisms underlying SNAI1 function in gliomas need further investigation.
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[Analysis of sialic acid in infant formulas using ultra performance liquid chromatography-triple quadrupole mass spectrometry].
Se Pu
PUBLISHED: 12-28-2013
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The method for analysing sialic acid in infant formulas by ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) has been established. Sialic acid in milk was released via acid hydrolysis, and purified by an HLB solid phase extraction cartridge. The UPLC separation was performed on an ACQUITY UPLC BEH HILIC column (50 mm x 2.1 mm, 1.7 microm) utilizing a gradient elution program of acetonitrile and water (containing 0.1% formic acid) as the mobile phases at a flow rate of 0.25 mL/min. Injection volume and column temperature were set at 5 microL and 30 degrees C, respectively. The identification and quantification were achieved by using electrospray ionisation (ESI)-MS/MS in positive ion mode and multiple reaction monitoring (MRM) mode. The linear range was from 0.05 to 5.0 mg/L for sialic acid and the correlation coefficient (R(2)) was greater than 0.99. The average recoveries spiked at the four concentration levels of 0.1, 0.5, 2.5 and 5.0 mg/L ranged between 84.3% and 98.9% with the relative standard deviations from 4.9% to 8.2%. The limit of detection was 0.01 mg/L. Therefore, this method has the characteristics of simple operation, high reproducibility and sensitivity. It can be widely applied to determine the total contents of sialic acid in infant formula, cow milk and human milk.
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[Interim report of prospective clinical study of two different digestive tract reconstruction after total gastrectomy].
Zhonghua Wei Chang Wai Ke Za Zhi
PUBLISHED: 12-27-2013
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To compare post-operative long-term complications and quality of life of two digestive reconstruction procedures after total gastrectomy.
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[Preventive administration of antibiotics to patients with benign prostatic hyperplasia before urodynamic examination].
Zhonghua Nan Ke Xue
PUBLISHED: 12-18-2013
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To discuss the effect and safety of preventive administration of antibiotics to patients with benign prostatic hyperplasia (BPH) before urodynamic examination.
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[Effect of quaternary ammonium methacrylates incorporation into a dental adhesive on the resistance of enzymatic degradation of resin-dentine interfaces].
Zhonghua Kou Qiang Yi Xue Za Zhi
PUBLISHED: 11-23-2013
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To evaluate the effect of quaternary ammonium methacrylates incorporation into a dental adhesive on the resistance of enzymatic degradation of resin-dentine interfaces.
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High resolution imaging with differential infrared absorption micro-spectroscopy.
Opt Express
PUBLISHED: 11-13-2013
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Although confocal infrared (IR) absorption micro-spectroscopy is well established for far-field chemical imaging, its scope remains restricted since diffraction limits the spatial resolution to values a little above half the radiation wavelength. Yet, the successful implementations of below-the-diffraction limit far-field fluorescence microscopies using saturated irradiation patterns for example for stimulated-emission depletion and saturated structured-illumination suggest the possibility of using a similar optical patterning strategy for infrared absorption mapping at high resolution. Simulations are used to show that the simple mapping of the difference in transmitted/reflected IR energy between a saturated vortex-shaped beam and a Gaussian reference with a confocal microscope affords the generation of high-resolution vibrational absorption images. On the basis of experimentally relevant parameters, the simulations of the differential absorption scheme reveal a spatial resolution better than a tenth of the wavelength for incident energies about a decade above the saturation threshold. The saturated structured illumination concepts are thus expected to be compatible with the establishment of point-like point-spread functions for measuring the absorbance of samples with a scanning confocal microscope recording the differential transmission/reflection.
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The Thellungiella salsuginea Tonoplast Aquaporin TsTIP1;2 Functions in Protection Against Multiple Abiotic Stresses.
Plant Cell Physiol.
PUBLISHED: 11-09-2013
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Examination of aquaporin (AQP) membrane channels in extremophile plants may increase our understanding of plant tolerance to high salt, drought or other conditions. Here, we cloned a tonoplast AQP gene (TsTIP1;2) from the halophyte Thellungiella salsuginea and characterized its biological functions. TsTIP1;2 transcripts accumulate to high levels in several organs, increasing in response to multiple external stimuli. Ectopic overexpression of TsTIP1;2 in Arabidopsis significantly increased plant tolerance to drought, salt and oxidative stresses. TsTIP1;2 had water channel activity when expressed in Xenopus oocytes. TsTIP1;2 was also able to conduct H2O2 molecules into yeast cells in response to oxidative stress. TsTIP1;2 was not permeable to Na(+) in Xenopus oocytes, but it could facilitate the entry of Na(+) ions into plant cell vacuoles by an indirect process under high-salinity conditions. Collectively, these data showed that TsTIP1;2 could mediate the conduction of both H2O and H2O2 across membranes, and may act as a multifunctional contributor to survival of T. salsuginea in highly stressful habitats.
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HOTAIR, a cell cycle-associated long noncoding RNA and a strong predictor of survival, is preferentially expressed in classical and mesenchymal glioma.
Neuro-oncology
PUBLISHED: 11-07-2013
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Long noncoding RNA Hox transcript antisense intergenic RNA (HOTAIR) has been characterized as a negative prognostic factor in breast and colon cancer patients. The clinical significance and function of HOTAIR in glioma remains unclear.
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Phase difference in modulated signals of two orthogonally polarized outputs of a Nd:YAG microchip laser with anisotropic optical feedback.
Opt Lett
PUBLISHED: 11-02-2013
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We present an experimental observation of the output responses of a Nd:YAG microchip laser with an anisotropic external cavity under weak optical feedback. The feedback mirror is stationary during the experiments. A pair of acousto-optic modulators is used to produce a frequency shift in the feedback light with respect to the initial light. The laser output is a beat signal with 40 kHz modulation frequency and is separated into two orthogonal directions by a Wollaston prism. Phase differences between the two intensity curves are observed as the laser works in two orthogonal modes, and vary with the external birefringence element and the pump power. Theoretical analyses are given, and the simulated results are consistent with the experimental phenomena.
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Potential molecular mechanisms for improved prognosis and outcome with neoadjuvant chemotherapy prior to laparoscopical radical hysterectomy for patients with cervical cancer.
Cell. Physiol. Biochem.
PUBLISHED: 10-23-2013
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Background: The p53:miR-34a:E2F positive feed-forward loop and the p53:miR-605:Mdm2 positive feed-back loop have been identified to be crucial oncogenesis/tumor suppressor-regulating signaling pathways. In this study, we sought to examine the hypothesis that neoadjuvant chemotherapy (NAC) is a better approach with improved prognosis and outcomes after laparoscopical radical hysterectomy (LRH) on patients with cervical cancer and to elucidate the potential roles of the p53:miR-34a:E2F1 and the p53:miR-605:Mdm2 signaling pathways in this therapy. Methods: Twenty-one patients with stage IIB cervical cancer were recruited to this study and they were randomly divided into two groups: LRH (n=10) and NAC+LRH (n=11) groups. The NAC+LRH group consisted of 4 cycles of cisplatin, paclitaxel and carboplatin. Complication rates and NAC outcomes (tumor size changes, 2-year disease-free survival rate, and 2-year overall survival rate) were compared between the two groups. Expression of p53, Mdm2, E2F1, miR-34a, and miR-605 at mRNA and protein levels from the tumor tissues was analyzed. Results: We observed that the diameter of tumors following chemotherapy was substantially smaller in the NAC+LRH patients than in LRH patients. No recurrence or metastasis after surgery was observed in the NAC+LRH patients, whereas 2 out of 10 LRH patients had recurrences and 1 had metastasis. The 2-year disease-free and overall survival rates were apparently higher in the NAC+LRH group than in the LRH group. Furthermore, molecular biology analyses revealed that the protein and mRNA levels of p53 were both markedly increased in patients who received NAC than those who did not, and oppositely, the levels of E2F1 and Mdm2 were significantly lower in the NAC+LRH patients than in the LRH patients. The levels of miR-34a and miR-605 were considerably higher with NAC relative to without NAC. Among the three anti-cancer drugs included in NAC, cisplatin was found to be the main component that caused increases in p53 protein levels, miR-34a and miR-605 miRNA levels, and decreases in Mdm2 and E2F1 protein levels. Furthermore, ERK1/2 inhibitor U0126 or TAB1 siRNA mitigated these changes induced by cisplatin. Conclusion: These findings not only indicate NAC as a rational approach for better treatment of cervical cancer with improved therapeutic outcomes, due partly to the ability of cisplatin to promote the p53:miR-34a:E2F1 positive feed-forward loop and the p53:miR-605:Mdm2 positive feedback loop. © 2014 S. Karger AG, Basel.
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Description of a new Pratylenchus species from China (Tylenchida, Pratylenchidae).
Zool. Res.
PUBLISHED: 10-12-2013
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Pratylenchus ekrami from maize (Zea mays) roots in Shenyang and luffa (Luffa cylindrica) roots in Dalian, China, are described in this paper. Nematodes from the two areas were identified consistently, and were characterized by a heavy cephalic sclerotization, extending posteriorly up to two body annuli, stylet 11-13 ?m long, elongating conoid tail, and becoming thinner from vulva. Males were not found. Pratylenchus ekrami is close to Pratylenchus vulnus, but the most critical characteristics between the two species were the number of lip annuli, stylet size, and shape of stylet knobs. This species is the first reported in China.
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Overexpressed let-7a inhibits glioma cell malignancy by directly targeting K-ras, independently of PTEN.
Neuro-oncology
PUBLISHED: 10-03-2013
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Altered expression of micro(mi)RNAs has been shown to be associated with tumorigenesis and tumor progression. The expression of phosphatase and tensin homolog (PTEN) plays an important role in glioma and is regarded as a prognostic marker of glioma patients. The goal of this study was to investigate the function of lethal (let)-7a miRNA in glioma cell lines with different PTEN phenotypes.
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[Investigate circulating levels of chemokines and evaluate the correlation between these chemokines and liver function indicators in autoimmune hepatitis].
Zhonghua Gan Zang Bing Za Zhi
PUBLISHED: 09-12-2013
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This study investigated circulation levels of chemokines (CCL2, CCL5, CXCL8, CXCL9, CXCL10) in autoimmune hepatitis(AIH) patients and evaluated the correlation between these chemokines and liver function indicators.
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Sodium fluoride induces apoptosis through the downregulation of hypoxia-inducible factor-1? in primary cultured rat chondrocytes.
Int. J. Mol. Med.
PUBLISHED: 08-21-2013
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It has been reported that sodium fluoride (NaF) suppresses the proliferation and induces apoptosis of chondrocytes. However, the cellular and molecular mechanisms of the effect have not been elucidated. Therefore, the aim of this study was to evaluate the mechanisms of the effects of NaF on primary cultured rat chondrocytes in vitro. Chondrocytes were treated with NaF at concentrations of 0, 1.5, 2.0, 2.5, 3.0, 3.5 and 4.0 mM. Cell viability decreased and the rate of apoptotic cells increased significantly with the gradient concentration of NaF in a time- and dose-dependent manner. Electron microscopy revealed cytoplasmic, organelle and nuclear alterations in the ultrastructure of chondrocytes exposed to various NaF concentrations. The cell cycle distribution was analyzed by flow cytometry, and the results indicated that NaF induced G2 cell cycle arrest. Western blotting was used to detect the apoptotic pathways. Downregulation of the Bcl-2 protein and upregulation of Bax, cleaved caspase-9, -12 and -3 proteins suggested that NaF was capable of inducing apoptosis through the mitochondrial and endoplasmic reticulum pathways. The results also showed that the levels of hypoxia-inducible factor 1? (HIF-1?), sex determining region Y box gene 9 (Sox9) and the collagen II (Col II) protein of the NaF groups were lower compared to those of the control groups. Thus, NaF may induce apoptosis through the downregulation of HIF-1? and disrupt the synthesis of extracellular matrix (ECM) through the downregulation of HIF-1? via the Sox9 pathway in primary cultured rat chondrocytes.
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The Protective Effect of Cordymin, a Peptide Purified from the Medicinal Mushroom Cordyceps sinensis, on Diabetic Osteopenia in Alloxan-Induced Diabetic Rats.
Evid Based Complement Alternat Med
PUBLISHED: 08-17-2013
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The aim of this study was to investigate the protective effect of cordymin on diabetic osteopenia in alloxan-induced diabetic rats and the possible mechanisms involved. The diabetic rats received daily intraperitoneal injection with cordymin (20, 50, and 100?mg/kg/day) for 5 weeks. Cordymin could restore the circulating blood glucose, glycosylated hemoglobin (HbA1c), serum alkaline phosphatase (ALP), tartrate resistant acid phosphatase (TRAP), and insulin levels in a dose-dependent manner. Also, the treatment of diabetic rats with cordymin could partially reverse the ? cells death and decrease the total antioxidant status (TAOS) in the diabetic rats. The results may directly and indirectly account for the possible mechanism of the beneficial effect of cordymin on diabetic osteopenia, which was confirmed with the increased bone mineral content (BMC) and bone mineral density (BMD) in diabetic rats (P < 0.05). All those findings indicate that cordymin may play a protective role in diabetic osteoporosis.
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CMPO-calix[4]arenes with spacer containing intramolecular hydrogen bonding: Effect of local rigidification on solvent extraction toward f-block elements.
J. Hazard. Mater.
PUBLISHED: 08-12-2013
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To understand intramolecular hydrogen bonding in effecting liquid-liquid extraction behavior of CMPO-calixarenes, three CMPO-modified calix[4]arenes (CMPO-CA) 5a-5c with hydrogen-bonded spacer were designed and synthesized. The impact of spacer rotation that is hindered by introduction of intramolecular hydrogen bonding upon extraction of La(3+), Eu(3+), Yb(3+), Th(4+), and UO2(2+) has been examined. The results show that 5b and 5c containing only one hydrogen bond with a less hindered rotation spacer extract La(3+) more efficiently than 5a containing two hydrogen bonds with a more hindered rotation spacer, demonstrating the importance of local rigidification of spacer in the design of extractants in influencing the coordination environment. The large difference in extractability between La(3+) and Yb(3+) (or Eu(3+)) by 5b (or 5c), and the small difference by 5a, suggests intramolecular hydrogen bonding do exert pronounced influence upon selective extraction of light and heavy lanthanides. Log-log plot analysis indicates a 1:1, 2:1 and 1:1 stoichiometry (ligand/metal) for the extracted complex formed between 5b and La(3+), Th(4+), UO2(2+), respectively. Additionally, their corresponding acyclic analogs 7a-7c exhibit negligible extraction toward these metal ions. These results reveal the possibility of selective extraction via tuning local chelating surroundings of CMPO-CA by aid of intramolecular hydrogen bonding.
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Four distinct types of dehydration stress memory genes in Arabidopsis thaliana.
BMC Plant Biol.
PUBLISHED: 08-11-2013
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How plants respond to dehydration stress has been extensively researched. However, how plants respond to multiple consecutive stresses is virtually unknown. Pre-exposure to various abiotic stresses (including dehydration) may alter plants subsequent responses by improving resistance to future exposures. These observations have led to the concept of stress memory implying that during subsequent exposures plants provide responses that are different from those during their first encounter with the stress. Genes that provide altered responses in a subsequent stress define the memory genes category; genes responding similarly to each stress form the non-memory category.
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What is Visualize?

JoVE Visualize is a tool created to match the last 5 years of PubMed publications to methods in JoVE's video library.

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In developing our video relationships, we compare around 5 million PubMed articles to our library of over 4,500 methods videos. In some cases the language used in the PubMed abstracts makes matching that content to a JoVE video difficult. In other cases, there happens not to be any content in our video library that is relevant to the topic of a given abstract. In these cases, our algorithms are trying their best to display videos with relevant content, which can sometimes result in matched videos with only a slight relation.