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Find video protocols related to scientific articles indexed in Pubmed.
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Effect of thermocycling on roughness of nanofill, microfill and microhybrid composites.
Acta Odontol. Scand.
PUBLISHED: 10-31-2014
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Abstract Objective. This study aimed to investigate the surface roughness of composite resins subjected to thermal cycles procedure. Materials and methods. Two microfill, four microhybrid and four nanofill composites were used. The surface roughness (Ra) was initially measured in a profilometer using a cut-off 0f 0.25 mm, after 3000 and 10,000 thermal cycles. Data were subjected to ANOVA and Fischer's test (? = 0.05). Results. Overall, 3000 thermal cycles increased the surface roughness values for all materials and there was a trend in all groups to decrease the roughness after 10,000 thermal cycles. Conclusions. The composition of material, including the type of organic matrix, could be more relevant to roughness maintenance over time than the general behavior of composites based on particles fillers. The maintenance of smooth surface in resin-based composite restorations is totally dependent of organic composition of the material.
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The effects of chronic stress on hippocampal adult neurogenesis and dendritic plasticity are reversed by selective MAO-A inhibition.
J. Psychopharmacol. (Oxford)
PUBLISHED: 10-14-2014
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There is accumulating evidence that adult neurogenesis and dendritic plasticity in the hippocampus are neuroplastic phenomena, highly sensitive to the effects of chronic stress and treatment with most classes of antidepressant drugs, being involved in the onset and recovery from depression. However, the effects of antidepressants that act through the selective inhibition of monoamine oxidase subtype A (MAO-A) in these phenomena are still largely unknown. In the present study, adult neurogenesis and neuronal morphology were examined in the hippocampus of rats exposed to chronic mild stress (CMS) and treated with the selective reversible MAO-A inhibitor (RIMA) drug, pirlindole and the selective serotonin reuptake inhibitor (SSRI), fluoxetine. The results provide the first demonstration that selective MAO-A inhibition with pirlindole is able to revert the behavioural effects of stress exposure while promoting hippocampal adult neurogenesis and rescuing the stress-induced dendritic atrophy of granule neurons.
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Aerobic exercise did not have compensatory effects on physical activity levels in type 2 diabetes patients.
J Sports Sci
PUBLISHED: 09-27-2014
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Abstract Although exercise promotes beneficial effects in diabetic patients, some studies have questioned the degree of their importance in terms of the increase in total energy expenditure. In these studies, the decrease of physical activity levels (PAL) was referred as "compensatory effect of exercise". However, our aim was to investigate whether aerobic exercise has compensatory effects on PAL in type 2 diabetes patients. Eight volunteers (51.1 ± 8.2 years) were enrolled in a supervised exercise programme for 8 weeks (3 d · wk(-1), 50-60% of VO2 peak for 30-60 min). PAL was measured using tri-axial accelerometers in the 1st, 8th and 12th weeks. Biochemical tests, cardiorespiratory fitness, anthropometric assessment and body composition were measured in the 2nd and 11th weeks. Statistical analysis was performed using non-parametric tests (Friedman and Wilcoxon, P < 0.05). We found no significant differences in PAL between intervention periods, and participants spent the majority of their awake time in sedentary activities. However, the exercise programme generated a significant 14.8% increase in VO2 peak and a 15% reduction in fructosamine. The exercise programme had no compensatory effects on PAL in type 2 diabetes patients, but improved their cardiorespiratory fitness and glycaemic control.
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Instrumented hip joint replacements, femoral replacements and femoral fracture stabilizers.
Expert Rev Med Devices
PUBLISHED: 09-19-2014
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This paper reviews instrumented hip joint replacements, instrumented femoral replacements and instrumented femoral fracture stabilizers. Examination of the evolution of such implants was carried out, including the detailed analysis of 16 architectures, designed by 8 research teams and implanted in 32 patients. Their power supply, measurement, communication, processing and actuation systems were reviewed, as were the tests carried out to evaluate their performance and safety. These instrumented implants were only designed to measure biomechanical and thermodynamic quantities in vivo, in order to use such data to conduct research projects and optimize rehabilitation processes. The most promising trend is to minimize aseptic loosening and/or infection following hip or femoral replacements or femoral stabilization procedures by using therapeutic actuators inside instrumented implants to apply controlled stimuli in the bone-implant interface.
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Histamine H4 Receptor Ligands: Future Applications and State of Art.
Chem Biol Drug Des
PUBLISHED: 09-18-2014
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Histamine is a chemical transmitter found practically in whole organism and exerts its effects through the interaction with H1 to H4 histaminergic receptors. Specifically, H4 receptors are found mainly in immune cells and blood-forming tissues, thus are involved in inflammatory and immune processes, as well as some actions in central nervous system. Therefore, H4 receptor ligands can have applications in the treatment of chronic inflammatory and immune diseases and may be novel therapeutic option in these conditions. Several H4 receptor ligands have been described from early 2000's until nowadays, being imidazole, indolecarboxamide, 2-aminopyrimidine, quinazoline, and quinoxaline scaffolds the most explored and discussed in this review. Moreover, several studies of molecular modeling using homology models of H4 receptor and QSAR data of the ligands are summarized. The increasing and promising therapeutic applications are leading these compounds to clinical trials, which probably will be part of the next generation of blockbuster drugs.
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The MYLIP p.N342S polymorphism is associated with response to lipid-lowering therapy in Brazilian patients with familial hypercholesterolemia.
Pharmacogenet. Genomics
PUBLISHED: 08-30-2014
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A previous study reported that the myosin regulatory light chain interacting protein (MYLIP) might serve as a novel therapeutic class for treating dyslipidemia. It contributes to variations in the levels of circulating low-density lipoprotein cholesterol (LDL-C), promoting the degradation of LDL-LDLR, thus limiting absorption. The effect of genetic variation in the MYLIP gene in a disease scenario characterized by mutations in the LDLR gene has not been previously evaluated.
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Synthesis and evaluation of a pyrazinoic acid prodrug in Mycobacterium tuberculosis.
Saudi Pharm J
PUBLISHED: 08-28-2014
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Tuberculosis (TB) is a disease caused mainly by infection of Mycobacterium tuberculosis affecting more than ten million people around the world. Despite TB can be treated, the rise of MDR-TB and XDR-TB cases put the disease in a worrying status. As pyrazinamide-resistant strains exhibit low or none pyrazinamidase activity, it is proposed that the active form of pyrazinamide (PZA) is pyrazinoic acid (POA), although this acid has poor penetration in mycobacteria. In this work, we present a convenient one-pot synthesis of 2-chloroethyl pyrazinoate, and its activity in M. tuberculosis H37Rv (ATCC27294) in MIC assay using the MABA technique. The obtained MIC of the compound was 3.96 g/mL, and discussion about the activity profile of some previously evaluated pyrazinoates is also performed.
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3-alkoxy-pyrrolo[1,2-b]pyrazolines as selective androgen receptor modulators with ideal physicochemical properties for transdermal administration.
J. Med. Chem.
PUBLISHED: 08-26-2014
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We describe the synthesis and characterization of 3-alkoxy-pyrrolo[1,2-b]pyrazolines as novel selective androgen receptor (AR) modulators that possess excellent physicochemical properties for transdermal administration. Compound 26 bound to human AR with an IC50 of 0.7 nM with great selectivity over other nuclear hormone receptors and potently activated AR in a C2C12 muscle cell reporter gene assay with an EC50 of 0.5 nM. It showed high aqueous solubility of 1.3 g/L at pH 7.4, and an in silico model as well as a customized parallel artificial membrane permeability assay indicated good skin permeation. Indeed, when measuring skin permeation through excised human skin, an excellent flux of 2 ?g/(cm(2)·h) was determined without any permeation enhancers. In a 2 week Hershberger model using castrated rats, the compound showed dose-dependent effects fully restoring skeletal muscle weight at 0.3 mg/kg/day after subcutaneous administration with high selectivity over prostate stimulation.
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First records of Ochrotrichia Mosely, 1934 (Trichoptera: Hydroptilidae) in Northeastern Brazil: Five new species and two new geographical records.
Zootaxa
PUBLISHED: 08-13-2014
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About 170 species have been assigned to the New World genus Ochrotrichia, only two of which were previously recorded from Brazil. Material collected in Bahia and Ceará states represent the first records of this genus from Northeastern Brazil and reveal five new species of Ochrotrichia. These are described and illustrated in this paper: O. caatinga sp. nov. (O. aldama group, from Ceará), O. priapo sp. nov. (O. aldama group, from Bahia); O. limeirai sp. nov. (O. aldama group, from Ceará), O. constricta sp. nov. (undetermined group, from Bahia), and O. igrapiuna sp. nov. (O. xena group, from Bahia). Ochrotrichia manuensis Flint & Bueno-Soria 1999 is firstly recorded from Brazil and O. patulosa (Wasmund & Holzenthal 2007) is firstly recorded from Northeastern Brazil. 
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Helicopsyche (Feropsyche) timbira sp. nov. (Trichoptera: Helicopsychidae), a new species from southeastern Brazil.
Zootaxa
PUBLISHED: 08-08-2014
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The family Helicopsychidae comprises only two genera, the monotypic Rakiura McFarlane 1973 and the diverse Helicopsyche von Siebold 1856. Johanson (1998) provided a phylogenetic and biogeographic analysis for Helicopsyche, and ranked all other previously Helicopsychidae genera as subgenera of Helicopsyche. Currently, Helicopsyche is subdivided into six subgenera: Helicopsyche, Petrotrichia Ulmer 1910, Galeopsyche Johanson 1998, Saetotrichia Brauer 1865, Cochliopsyche Müller 1885, and Feropsyche Johanson 1998. Johanson (2002, 2003) provided revisions to Neotropical species of Helicopsyche. These species belong to the subgenus Cochliopsyche (currently with 16 species) or to the subgenus Feropsyche (with about 100 species), both endemic to the Neotropics. According to Johanson & Malm (2006), the Neotropical fauna can be divided into three regions: One that includes the West Indies, where 33 Helicopsyche species are recorded, another including Central America, with 25 Helicopsyche species, and finally, another one that includes South America, with 41 Helicopsyche species. In Brazil, only 19 species are recorded, of which six are from the southeastern region (Santos et al. 2013). However, the Brazilian fauna is underestimated and possibly much richer than these numbers indicate, especially when it is compared to regions of Central America or South America, such as Venezuela (28 spp.) and Mexico (18 spp.) (Johanson & Malm 2006). In this work, a new species of Helicopsyche (Feropsyche) is described from southeastern Brazil. Specimens were collected at Serra dos Órgãos (Rio de Janeiro State), a mountain range that includes a large remnant of the Atlantic Forest in Brazil. Additional specimens were identified from São Paulo State, in an area that, along with the Serra dos Órgãos, is a part of the forest corridor of the mountain range named Serra do Mar. 
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Physical Activity and Better Medication Compliance Improve Mini-Mental State Examination Scores in the Elderly.
Dement Geriatr Cogn Disord
PUBLISHED: 08-04-2014
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In addition to hypertension, dyslipidemia, atherosclerosis, and diabetes, a sedentary lifestyle plays a pivotal role in cerebro- and cardiovascular disease and progressive cognitive decline, including vascular dementia and Alzheimer's disease. The present study investigated whether controlling the key risks and participating in physical activity have a beneficial impact on these disorders. Elderly volunteers were enrolled in a 3-month program that consisted of structured exercise three times per week. The daily routine, medical treatment, and vital parameters were evaluated and correlated with the subjects' neuropsychiatric status. High blood pressure was found in 40% of the participants, with no significant differences between the sexes. A higher proportion of females (55%) than males (18%) forgot to take their medication during the observation period. Significant negative correlations were found between Mini-Mental State Examination (MMSE) scores and age, lack of a caregiver, and increased pulse rate before or after exercise. These results suggest that the presence of home assistance and subsequent improvement in medication compliance, vital parameter optimization, and regular physical activity may yield better MMSE results and a lower risk for cerebro- and cardiovascular disease. © 2014 S. Karger AG, Basel.
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Role of microglia adenosine A(2A) receptors in retinal and brain neurodegenerative diseases.
Mediators Inflamm.
PUBLISHED: 07-16-2014
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Neuroinflammation mediated by microglial cells in the brain has been commonly associated with neurodegenerative diseases. Whether this microglia-mediated neuroinflammation is cause or consequence of neurodegeneration is still a matter of controversy. However, it is unequivocal that chronic neuroinflammation plays a role in disease progression and halting that process represents a potential therapeutic strategy. The neuromodulator adenosine emerges as a promising targeting candidate based on its ability to regulate microglial proliferation, chemotaxis, and reactivity through the activation of its G protein coupled A2A receptor (A2AR). This is in striking agreement with the ability of A2AR blockade to control several brain diseases. Retinal degenerative diseases have been also associated with microglia-mediated neuroinflammation, but the role of A2AR has been scarcely explored. This review aims to compare inflammatory features of Parkinson's and Alzheimer's diseases with glaucoma and diabetic retinopathy, discussing the therapeutic potential of A2AR in these degenerative conditions.
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[Evaluation of the accessibility to the Specialized Dental Care Center within the scope of the macro region of Sobral, in the state of Ceará, Brazil].
Cien Saude Colet
PUBLISHED: 07-12-2014
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The Specialized Dental Care Center for the region of Sobral in the state of Ceará (CEO-R) has characteristics that render it unique, as it has regional coverage and is managed by a Local Health Consortium (which comprises 24 cities). It also works in conjunction with the School of Dentistry of the Federal University of Ceará - Sobral Campus, combining care and academic study in the same location. The scope of this article was to evaluate the accessibility to specialized care in this region with the implementation of this CEO-R. A descriptive-exploratory study with a quantitative approach was conducted by means of the collection and analysis of secondary data regarding the availability and usage of this service in addition to the application of semi-structured interviews with Oral Health Coordinators of the 24 municipalities in the consortium. The results showed that the CEO-R is equipped to meet the demand. However, geographic, financial and organizational barriers were detected, which explains the low rates of appointments scheduled and use of the services and the high rate of missed appointments, causing a significant financial impact on the cities included in the consortium.
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Report of four cases of Bardet-Biedl syndrome.
J Bras Nefrol
PUBLISHED: 06-02-2014
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Bardet-Biedl syndrome is rare. Although its diagnosis depends on cardinal clinical manifestations which appear in childhood, we report four cases of Bardet-Biedl syndrome lately diagnosed in a dialysis center. Three cases were diagnosed in end-stage renal disease patients when they started maintenance hemodialysis, and one case was diagnosed through screening among hemodialysis patients' relatives. Although pediatricians have more opportunity to diagnose the syndrome, nephrologists are important during the treatment, since renal failure is the main cause of death among Bardet-Biedl syndrome patients. Moreover, late diagnosis of the syndrome among patients with end-stage renal disease can help to detect new cases through the screening among hemodialysis patients' relatives.
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Preventive health services implemented by family physicians in Portugal-a cross-sectional study based on two clinical scenarios.
BMJ Open
PUBLISHED: 05-28-2014
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To assess whether Portuguese family physicians perform preventive health services in accordance with scientific evidence, based on the recommendations of the United States Preventive Services Task Force (USPSTF).
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Monte Carlo simulation of laser beams interaction with the human eye using Geant4.
Biomed Eng Online
PUBLISHED: 04-29-2014
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Due to the unique characteristics of the eye, ophthalmologic diagnostic techniques often rely on the photons interaction with the retina to infer its internal structure. Although these techniques are widely used, the interpretation of the generated images is not always fully understood, as in scanning laser ophthalmoscopy dark field imaging. This limits the exploitation of its full potential as a diagnostic tool for deep abnormalities in the retina, as in the situation of drusen.
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Surgical techniques for maxillary bone grafting - literature review.
Rev Col Bras Cir
PUBLISHED: 04-29-2014
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For oral rehabilitation with implant-supported prostheses, there are required procedures to create the bone volume needed for installation of the implants. Thus, bone grafts from intraoral or extraoral donor sites represent a very favorable opportunity. This study aimed to review the literature on the subject, seeking to discuss parameters for the indications, advantages and complications of techniques for autogenous bone grafts.
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Histopathologic diagnosis of chronic graft-versus-host disease of the oral mucosa according to the National Institutes of Health Consensus.
Einstein (Sao Paulo)
PUBLISHED: 04-15-2014
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To validate the minimal criteria of the histopathologic diagnosis of oral chronic graft-versus-host disease, based on the histopathologic classification of the National Institutes of Health and correlate them with clinical features.
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Seasonal variations cause morphological changes and altered spermatogenesis in the testes of viscacha (Lagostomus maximus).
Anim. Reprod. Sci.
PUBLISHED: 04-14-2014
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This study complements the previous investigations of the reproductive biology of male viscachas, a rodent of a seasonal Hystricognathi that exhibits photoperiod-induced morphological variations in the reproductive system. In the present study, a quantitative analysis of spermatogenesis was performed during the summer and the spring. Spermatogonial cells were analyzed to determine by immunolabelling for STRA8 and DAZL, which are essential for spermatogenesis. Six free-living male viscachas were captured, three animals in the summer during the period of reproductive activity and three animals in the spring during the period of testicular regression. The testes of the viscachas were collected and processed for light microscopy, macroscopic and immunochemical analyses. The germ and Sertoli cells present in the seminiferous tubules were quantitatively analyzed in each animal. The efficiency coefficient for spermatogonial mitosis, meiotic yield, overall spermatogenesis yield and Sertoli cell index, revealed that the Sertoli cells in male viscachas captured during the summer had a reduced capacity to structurally and nutritionally support the developing round spermatids compared with the male viscachas captured during the spring. The animals produced less sperm during the spring than the summer, suggesting a seasonal impact on spermatogenesis. Immunolabelling for STRA8 and DAZL was detected during summer and spring seasons. These results suggest that in seasonal rodents, such as the male viscachas, the photoperiod promotes significant changes in the testis and in the germ cell yield.
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ACTN3 R577X polymorphism and long-term survival in patients with chronic heart failure.
BMC Cardiovasc Disord
PUBLISHED: 04-10-2014
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Previous studies have shown the occurrence of actinin-3 deficiency in the presence of the R577X polymorphism in the ACTN3 gene. Our hypothesis is that this deficiency, by interfering with the function of skeletal muscle fiber, can result in a worse prognosis in patients with chronic heart failure.
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Transition from cyclosporine-induced renal dysfunction to nephrotoxicity in an in vivo rat model.
Int J Mol Sci
PUBLISHED: 04-07-2014
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Cyclosporin A (CsA), a calcineurin inhibitor, remain the cornerstone of immunosuppressive regimens, regardless of nephrotoxicity, which depends on the duration of drug exposure. The mechanisms and biomarkers underlying the transition from CsA-induced renal dysfunction to nephrotoxicity deserve better elucidation, and would help clinical decisions. This study aimed to clarify these issues, using a rat model of short- and long-term CsA (5 mg/kg bw/day) treatments (3 and 9 weeks, respectively). Renal function was assessed on serum and urine; kidney tissue was used for histopathological characterization and gene and/or protein expression of markers of proliferation, fibrosis and inflammation. In the short-term, creatinine and blood urea nitrogen (BUN) levels increased and clearances decreased, accompanied by glomerular filtration rate (GFR) reduction, but without kidney lesions; at that stage, CsA exposure induced proliferating cell nuclear antigen (PCNA), transforming growth factor beta 1 (TGF-?1), factor nuclear kappa B (NF-??) and Tumor Protein P53 (TP53) kidney mRNA up-regulation. In the long-term treatment, renal dysfunction data was accompanied by glomerular and tubulointerstitial lesions, with remarkable kidney mRNA up-regulation of the mammalian target of rapamycin (mTOR) and the antigen identified by monoclonal antibody Ki-67 (Mki67), accompanied by mTOR protein overexpression. Transition from CsA-induced renal dysfunction to nephrotoxicity is accompanied by modification of molecular mechanisms and biomarkers, being mTOR one of the key players for kidney lesion evolution, thus suggesting, by mean of molecular evidences, that early CsA replacement by mTOR inhibitors is indeed the better therapeutic choice to prevent chronic allograft nephropathy.
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Simultaneous use of amiodarone influences warfarin maintenance dose but is not associated with adverse events.
J Manag Care Pharm
PUBLISHED: 04-02-2014
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Drug interaction studies selecting patients in a real-life setting are scarce, and most studies to date are characterized by a small sample size.
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Mechanical properties of components of the bonding interface in different regions of radicular dentin surfaces.
J Prosthet Dent
PUBLISHED: 03-11-2014
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The mechanical properties of the adhesive materials used in intraradicular treatments could vary according to the interaction between the restorative material and dentin substrate. An evaluation of these properties is essential to determine the success of the luting procedures performed on glass-fiber posts.
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Exploratory study comparing dysautonomia between asthmatic and non-asthmatic elite swimmers.
Rev Port Pneumol
PUBLISHED: 03-06-2014
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Dysautonomia has been independently associated with training and exercise-induced bronchoconstriction. In addition, neurogenic airway inflammation was recently associated with swimmers-asthma. We aimed to assess the relation between autonomic nervous system and airway responsiveness of asthmatic elite swimmers.
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Effects of continuous rate infusion of butorphanol in isoflurane-anesthetized calves.
Acta Cir Bras
PUBLISHED: 02-24-2014
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To assess the hemodynamic changes and bispectral index (BIS) following administration of a continuous rate infusion (CRI) of butorphanol in isoflurane-anesthetized calves.
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Conversion to sirolimus ameliorates cyclosporine-induced nephropathy in the rat: focus on serum, urine, gene, and protein renal expression biomarkers.
Biomed Res Int
PUBLISHED: 02-21-2014
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Protocols of conversion from cyclosporin A (CsA) to sirolimus (SRL) have been widely used in immunotherapy after transplantation to prevent CsA-induced nephropathy, but the molecular mechanisms underlying these protocols remain nuclear. This study aimed to identify the molecular pathways and putative biomarkers of CsA-to-SRL conversion in a rat model. Four animal groups (n = 6) were tested during 9 weeks: control, CsA, SRL, and conversion (CsA for 3 weeks followed by SRL for 6 weeks). Classical and emergent serum, urinary, and kidney tissue (gene and protein expression) markers were assessed. Renal lesions were analyzed in hematoxylin and eosin, periodic acid-Schiff, and Masson's trichrome stains. SRL-treated rats presented proteinuria and NGAL (serum and urinary) as the best markers of renal impairment. Short CsA treatment presented slight or even absent kidney lesions and TGF-?, NF- ??, mTOR, PCNA, TP53, KIM-1, and CTGF as relevant gene and protein changes. Prolonged CsA exposure aggravated renal damage, without clear changes on the traditional markers, but with changes in serums TGF- ? and IL-7, TBARs clearance, and kidney TGF-? and mTOR. Conversion to SRL prevented CsA-induced renal damage evolution (absent/mild grade lesions), while NGAL (serum versus urine) seems to be a feasible biomarker of CsA replacement to SRL.
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Disease severity affects obstacle crossing in people with Parkinson's disease.
Gait Posture
PUBLISHED: 02-21-2014
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The current study evaluated the effects of disease severity on the control of obstacle crossing in people with idiopathic Parkinson's disease (PD). Forty-five subjects participated in the study, including 15 patients with mild PD (classified as stage 1 to 1.5 of the Hoehn and Yahr Rating Scale), 15 patients with moderate PD (classified as stage 2 to 3 of the Hoehn and Yahr Rating Scale), and 15 neurologically healthy individuals. Groups were matched by sex, age, body mass, and body height. The obstacle crossing task required participants to walk along a pathway and step over an obstacle (half of the knee height, positioned in the middle of the pathway). Patients were tested in a typically medicated state. Kinematic data were recorded using an optoelectronic tridimensional system. The outcome measures included spatiotemporal measures of obstacle avoidance. There were no significant differences between patients with mild PD and healthy individuals. Patients with moderate PD exhibited shorter distances for leading toe clearance and leading foot placement after the obstacle than did healthy individuals. Patients with moderate PD tended to exhibit a lower leading horizontal mean velocity during obstacle crossing than did healthy individuals. We found significant negative relationships between obstacle crossing measures and disease severity (score on the motor section of the Unified Parkinson's Disease Rating Scale). These findings suggest that disease severity affects locomotor behavior during obstacle crossing in PD. Specifically, obstacle avoidance was not affected in the early stages of PD; however, bradykinesia and hypometria influenced obstacle crossing in patients with moderate PD.
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Oral compound nevus.
Dermatol. Online J.
PUBLISHED: 02-14-2014
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The melanocytic nevus is a benign and focal proliferation of nevus cells that can be congenital or acquired. Intraoral lesions are uncommon, and the etiology and pathogenesis are poorly understood. The occurrence rate of oral compound nevus is about 5.9% to 16.5% of all oral melanocytic nevi. A 22-year-old male patient presented with a dark brown macule on the buccal mucosa of the maxilla in the region of tooth 26. The lesion was elliptical, 0.7 x 0.5 cm, well circumscribed, asymptomatic, and the evolution time was unknown. An excisional biopsy was performed and microscopic analysis revealed nests of nevus cells in the epithelium and underlying connective tissue that were compatible with melanocytic compound nevus. Owing to the clinical similarity between oral melanocytic nevus and oral melanoma, a histopathological analysis is mandatory for definitive diagnosis.
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Influence of Ferrule, Post System, and Length on Stress Distribution of Weakened Root-filled Teeth.
J Endod
PUBLISHED: 02-11-2014
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The aim of this study was to evaluate the influence of a ferrule, post system, and length on the stress distribution of weakened root-filled teeth.
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Ethical implications and decision making in care education process.
Invest Educ Enferm
PUBLISHED: 02-10-2014
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To determine ethical implications for nursing practice at the point of decision making by nursing professors in practice area.
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Color alteration, hydrogen peroxide diffusion, and cytotoxicity caused by in-office bleaching protocols.
Clin Oral Investig
PUBLISHED: 01-31-2014
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This study evaluated the color alteration, cytotoxicity, and hydrogen peroxide (HP) diffusion associated with different in-office bleaching protocols.
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Sitagliptin prevents aggravation of endocrine and exocrine pancreatic damage in the Zucker Diabetic Fatty rat - focus on amelioration of metabolic profile and tissue cytoprotective properties.
Diabetol Metab Syndr
PUBLISHED: 01-30-2014
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The purpose of this study was to investigate some of the possible mechanisms underlying the protective effects of a dipeptidyl peptidase IV (DPP-IV) inhibitor, sitagliptin, on pancreatic tissue in an animal model of type 2 diabetes mellitus (T2DM), the Zucker Diabetic Fatty (ZDF) rat, focusing on glycaemic, insulinic and lipidic profiles, as well as, on apoptosis, inflammation, angiogenesis and proliferation mediators.
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Evaluation of bone heating, drill deformation, and drill roughness after implant osteotomy: guided surgery and classic drilling procedure.
Int J Oral Maxillofac Implants
PUBLISHED: 01-24-2014
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This study evaluated and compared bone heating, drill deformation, and drill roughness after several implant osteotomies in the guided surgery technique and the classic drilling procedure.
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Does ulnar variance change with age and what is the influence of training and biological characteristics in this change? A short-term longitudinal study in Portuguese artistic gymnasts.
Clin J Sport Med
PUBLISHED: 01-24-2014
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To analyze the variability and longitudinal stress-related changes in ulnar variance (UV) in a group of immature artistic gymnasts. The relationship between UV and a group of biological and training variables was also investigated.
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Interactions of age and leg muscle fatigue on unobstructed walking and obstacle crossing.
Gait Posture
PUBLISHED: 01-21-2014
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Older adults commonly report muscle fatigue, which may be associated with reduced walking ability. Elderly may have insufficient awareness of the balance threat caused by muscle fatigue. The aim of this study was to analyze the interaction effects of aging and leg muscle fatigue on gait parameters in walking and obstacle crossing. One hundred and twenty men, who were divided in six groups according to their age (20-29 years, 30-39 years, 40-49 years, 50-59 years, 60-69 years, above 70 years), participated in this study. Participants performed three trials of unobstructed level ground walking and obstacle crossing during walking before and after quadriceps muscle fatigue. To induce fatigue, participants performed a repeated sit-to-stand task from a chair with arms across the chest to a pre-determined cadence (30 cycles/min) using a metronome. Spatial-temporal gait parameters (stride length, duration, and speed, step width, and trailing and leading heel-clearance) were analyzed, and compared by two-way ANOVA (group and fatigue). The results confirmed our hypothesis, showing age-related effects of leg muscles fatigue in both gait conditions. From 40 years old, participants modulated spatial-temporal and vertical impulses in both tasks more in response to fatigue than younger participants, apparently to improve balance and safety. Leg muscle fatigue caused age-dependent changes in both unobstructed level ground walking and obstacle crossing during walking, which appeared to reflect an attempt to maintain balance and safety, probably to counteract adverse fatigue effects.
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Parkia pendula seed lectin: potential use to treat cutaneous wounds in healthy and immunocompromised mice.
Appl. Biochem. Biotechnol.
PUBLISHED: 01-15-2014
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Parkia pendula seed lectin was used to treat cutaneous wounds of normal and immunocompromised mice, inducing cicatrization. Methotrexate (0.8 mg/kg/week) was used as immunosuppressive drug. Wounds were produced in the dorsal region (1 cm(2)) of female albino Swiss mice (Mus musculus), health and immunocompromised. Wounds were daily topically treated with 100 ?L of the following solutions: (1) control (NaCl 0.15 M), (2) control Im (0.15 M NaCl), (3) P. pendula seed lectin (100 ?g/mL), and (4) P. pendula seed lectin Im (100 ?g/mL). Clinical evaluation was performed during 12 days. Biopsies for histopathology analysis and microbiological examinations were carried out in the second, seventh, and 12th days. The presence of edema and hyperemia was observed in all groups during inflammatory period. The first crust was detected from the second day, only in the groups treated with P. pendula seed lectin. Microbiological analysis of wounds from day 0 to day 2 did not show bacterium at P. pendula seed lectin group; however, Staphylococcus sp. was detected every day in the other groups. The lectin markedly induced a total wound closing at P. pendula seed lectin and P. pendula seed lectin Im groups on 11th day of evolution. The present study suggests that P. pendula seed lectin is a biomaterial potential to show pharmacological effect in the repair process of cutaneous wounds.
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Effect of glucans from Caripia montagnei mushroom on TNBS-induced colitis.
Int J Mol Sci
PUBLISHED: 01-13-2014
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In this study, we evaluated the effect of different doses of polysaccharides extracted from Caripia montagnei mushroom at different intervals of treatment on colonic injury in the model of colitis induced by 2,4,6-trinitrobenzene sulfonic acid (TNBS). The FT-IR analysis and NMR showed that the polysaccharides from this species of mushroom are composed of ?- and ?-glucans. The colonic damage was evaluated by macroscopic, histological, biochemical and immunologic analyses. The results showed the reduction of colonic lesions in all groups treated with the glucans. Such glucans significantly reduced the levels of IL-6 (50 and 75 mg/kg, p < 0.05), a major inflammatory cytokine. Biochemical analyses showed that the glucans from C. montagnei acted on reducing levels of alkaline phosphatase (75 mg/kg, p < 0.01) and myeloperoxidase (p < 0.001), a result confirmed by the reduction of cellular infiltration observed microscopically. The increase of catalase activity possibly indicates a protective effect of these glucans on colonic tissue, confirming their anti-inflammatory potential.
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Sitagliptin prevents inflammation and apoptotic cell death in the kidney of type 2 diabetic animals.
Mediators Inflamm.
PUBLISHED: 01-10-2014
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This study aimed to evaluate the efficacy of sitagliptin, a dipeptidyl peptidase IV (DPP-IV) inhibitor, in preventing the deleterious effects of diabetes on the kidney in an animal model of type 2 diabetes mellitus; the Zucker diabetic fatty (ZDF) rat: 20-week-old rats were treated with sitagliptin (10?mg/kg bw/day) during 6 weeks. Glycaemia and blood HbA1c levels were monitored, as well as kidney function and lesions. Kidney mRNA and/or protein content/distribution of DPP-IV, GLP-1, GLP-1R, TNF-?, IL-1?, BAX, Bcl-2, and Bid were evaluated by RT-PCR and/or western blotting/immunohistochemistry. Sitagliptin treatment improved glycaemic control, as reflected by the significantly reduced levels of glycaemia and HbA1c (by about 22.5% and 1.2%, resp.) and ameliorated tubulointerstitial and glomerular lesions. Sitagliptin prevented the diabetes-induced increase in DPP-IV levels and the decrease in GLP-1 levels in kidney. Sitagliptin increased colocalization of GLP-1 and GLP-1R in the diabetic kidney. Sitagliptin also decreased IL-1? and TNF-? levels, as well as, prevented the increase of BAX/Bcl-2 ratio, Bid protein levels, and TUNEL-positive cells which indicates protective effects against inflammation and proapoptotic state in the kidney of diabetic rats, respectively. In conclusion, sitagliptin might have a major role in preventing diabetic nephropathy evolution due to anti-inflammatory and antiapoptotic properties.
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Measuring skin temperature before, during and after exercise: a comparison of thermocouples and infrared thermography.
Physiol Meas
PUBLISHED: 01-07-2014
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Measuring skin temperature (TSK) provides important information about the complex thermal control system and could be interesting when carrying out studies about thermoregulation. The most common method to record TSK involves thermocouples at specific locations; however, the use of infrared thermal imaging (IRT) has increased. The two methods use different physical processes to measure TSK, and each has advantages and disadvantages. Therefore, the objective of this study was to compare the mean skin temperature (MTSK) measurements using thermocouples and IRT in three different situations: pre-exercise, exercise and post-exercise. Analysis of the residual scores in Bland-Altman plots showed poor agreement between the MTSK obtained using thermocouples and those using IRT. The averaged error was -0.75 °C during pre-exercise, 1.22 °C during exercise and -1.16 °C during post-exercise, and the reliability between the methods was low in the pre- (ICC = 0.75 [0.12 to 0.93]), during (ICC = 0.49 [-0.80 to 0.85]) and post-exercise (ICC = 0.35 [-1.22 to 0.81] conditions. Thus, there is poor correlation between the values of MTSK measured by thermocouples and IRT pre-exercise, exercise and post-exercise, and low reliability between the two forms of measurement.
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Presence and type of low density lipoprotein receptor (LDLR) mutation influences the lipid profile and response to lipid-lowering therapy in Brazilian patients with heterozygous familial hypercholesterolemia.
Atherosclerosis
PUBLISHED: 01-04-2014
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Familial hypercholesterolemia (FH) is an autosomal dominant disease caused mainly by LDLR mutations. This study assessed the influence of the presence and type of LDLR mutation on lipid profile and the response to lipid-lowering therapy in Brazilian patients with heterozygous FH.
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Alteration of sFAS and sFAS ligand expression during canine visceral leishmaniosis.
Vet. Parasitol.
PUBLISHED: 01-01-2014
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Visceral leishmaniosis (VL) is caused by intracellular parasites of the genus Leishmania that affect humans and several animal species. Dogs are one of the main urban reservoirs of Leishmania infantum and play a central role in the transmission cycle to humans via sandflies. CD3+ cells apoptosis is involved in the immune response in VL. Dysregulation of apoptosis has been implicated in various disease states. An important regulator of apoptosis is the FAS-FAS-associated death domain protein (cluster of differentiation 95 - CD95) and FASL-FAS ligand protein (cluster of differentiation 178 - CD178) system involved in the down-regulation of immune reactions and in T cell-mediated cytotoxicity. FAS is a member of the tumor necrosis factor (TNF) receptor super family, which can be expressed in transmembrane or soluble forms. The soluble levels of FAS (sFAS), FASL (sFASL) and active Caspase-3, this last related to apoptotic cascade, were investigated in the spleen of 19 symptomatic dogs presenting moderate VL and 6 healthy dogs, determined by ELISA assay. The splenic parasite load was determined by real-time PCR monitoring of amplification of the intergenic internal transcribed spacer (ITS1) gene of parasite rRNA. sFAS levels were lower (p<0.05). sFASL and active Caspase-3 levels were higher (p<0.05) in dogs with VL compared with controls. Negative correlation was observed between parasite burden and sFASL levels. The increase in sFASL could be related to the mechanism involved in the elimination of the parasite.
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Mandibular growth and dentoalveolar development in the treatment of Class II, division 1, malocclusion using Balters Bionator according to the skeletal maturation.
Dental Press J Orthod
PUBLISHED: 11-23-2013
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The purpose of the study was to evaluate the influence of the skeletal maturation in the mandibular and dentoalveolar growth and development during the Class II, division 1, malocclusion correction with Balters bionator.
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Rice fortified with iron given weekly increases hemoglobin levels and reduces anemia in infants: a community intervention trial.
Int J Vitam Nutr Res
PUBLISHED: 11-14-2013
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More than two billion people suffer from anemia worldwide, and it is estimated that more than 50 % of cases are caused by iron deficiency. In this community intervention trial, we evaluated infants aged 10 to 23 months of age (n = 171) from two public child day-care centers. Intervention lasted 18 weeks. The 50-g individual portion (uncooked) of fortified rice provided 56.4 mg of elemental iron as ferric pyrophosphate. Capillary blood samples to test for anemia were taken at baseline and at endpoint. The objective of this study was to evaluate the impact of rice fortified with iron (Ultrarice®) on hemoglobin and anemia prevalence compared with standard household rice. For the fortified rice center, baseline mean hemoglobin was 113.7 ± 9.2 g/L, and at endpoint 119.5 ± 7.7 g/L, p < 0.0001; for the standard rice center, baseline mean hemoglobin value was 113.5 ± 40.7 g/L, and at endpoint 113.6 ± 21.0, p = 0.99. Anemia prevalence for the fortified rice center was 27.8 % (20/72) at baseline, and 11.1 % (8/72) at endpoint, p = 0.012; for the control center, 47.1 % (33/70) were anemic at baseline, and 37.1 % (26/70) at the end of the study, p = 0.23. The Number Needed to Treat (NNT) was 4. In this intervention, rice fortified with iron given weekly was effective in increasing hemoglobin levels and reducing anemia in infants.
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Effect of the crown, post, and remaining coronal dentin on the biomechanical behavior of endodontically treated maxillary central incisors.
J Prosthet Dent
PUBLISHED: 11-05-2013
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It is unclear how the amount of remaining coronal dentin and the type of post and core rehabilitation affect the strain, stress distribution, and fracture resistance of endodontically treated teeth.
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The need for transparency and good practices in the qPCR literature.
Nat. Methods
PUBLISHED: 11-01-2013
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Two surveys of over 1,700 publications whose authors use quantitative real-time PCR (qPCR) reveal a lack of transparent and comprehensive reporting of essential technical information. Reporting standards are significantly improved in publications that cite the Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines, although such publications are still vastly outnumbered by those that do not.
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Electrocardiographic evaluation of the degree of sedation and the isolated use of methadone in healthy dogs.
Vet Anaesth Analg
PUBLISHED: 09-30-2013
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The present study aimed to investigate the influence of methadone on cardiorespiratory parameters, electrocardiogram and clinical sedation in dogs. Further possible side effects are reported.
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Torque stability of different abutment screws submitted to mechanical cycling.
Int J Oral Maxillofac Implants
PUBLISHED: 09-26-2013
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To evaluate the torque stability of different UCLA retention screws of single implant-supported crowns submitted to mechanical cycling.
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Investigation of genetic disturbances in oxygen sensing and erythropoietin signaling pathways in cases of idiopathic erythrocytosis.
Genet Res Int
PUBLISHED: 08-30-2013
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Background. Idiopathic erythrocytosis is the term reserved for cases with unexplained origins of abnormally increased hemoglobin after initial investigation. Extensive molecular investigation of genes associated with oxygen sensing and erythropoietin signaling pathways, in those cases, usually involves sequencing all of their exons and it may be time consuming. Aim. To perform a strategy for molecular investigation of patients with idiopathic erythrocytosis regarding oxygen sensing and erythropoietin signaling pathways. Methods. Samples of patients with idiopathic erythrocytosis were evaluated for the EPOR, VHL, PHD2, and HIF-2 ? genes using bidirectional sequencing of their hotspots. Results. One case was associated with HIF-2 ? mutation. Sequencing did not identify any pathogenic mutation in 4 of 5 cases studied in any of the studied genes. Three known nonpathogenic polymorphisms were found (VHL p.P25L, rs35460768; HIF-2 ? p.N636N, rs35606117; HIF-2 ? p.P579P, rs184760160). Conclusion. Extensive molecular investigation of cases considered as idiopathic erythrocytosis does not frequently change the treatment of the patient. However, we propose a complementary molecular investigation of those cases comprising genes associated with erythrocytosis phenotype to meet both academic and genetic counseling purposes.
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H-3, a new lectin from the marine sponge Haliclona caerulea: purification and mass spectrometric characterization.
Int. J. Biochem. Cell Biol.
PUBLISHED: 08-19-2013
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A new lectin from the marine sponge Haliclona caerulea (H-3) was isolated using a combination of hydrophobic interaction chromatography and ion-exchange chromatography. H-3 is a protein with three distinct bands on SDS-PAGE: 9 kDa, 16 kDa and 18 kDa. Nevertheless, on gel filtration and N-PAGE, H-3 showed a symmetrical peak and a unique band, respectively. Hemagglutinating activity of H-3 was stable at neutral pH and temperatures up to 60 °C. N-Acetylgalactosamine and porcine stomach mucin were the most potent inhibitors of H-3. Primary structure of the lectin was determined using tandem mass spectrometry, and it showed no similarity to any members of the animal lectin families. Top down fragmentation revealed some posttranslational modifications in H-3, including glycosylation. The glycan composition of H-3 was determined, and its structure was predicted. Furthermore, H-3 is a blue protein, binding to a chromophore(-597) by weak interactions, and this is the first time that the interaction between one lectin and a natural chromophore has been shown.
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Dyspepsia and gastric emptying in end-stage renal disease patients on hemodialysis.
BMC Nephrol
PUBLISHED: 08-18-2013
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Dyspepsia is common among end-stage renal disease (ESRD) patients and its association with delayed gastric emptying is not well established. We assessed the association of dyspepsia with gastric emptying time in ESRD patients undergoing hemodialysis (HD).
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Clinical and image findings in bisphosphonate-related osteonecrosis of the jaws.
J Craniofac Surg
PUBLISHED: 07-16-2013
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Bisphosphonate-related osteonecrosis of the jaws (BRONJ) is characterized as exposed bone in the jaws for more than 8 weeks in patients with current or previous history of therapy with bisphosphonates (BPs) and no history of radiotherapy in the head and neck. We report a case series of 7 patients with BRONJ and analyze the variations of clinical and imaging signs, correlating them with the presence or absence of bone exposure. Among the patients, 6 were women and 1 was a man, aged 42-79 years. Five of the patients were using zoledronic acid and the other 2 alendronate. The use of BPs varied from 3 to 13 years. In 5 patients, tooth extraction was the triggering event of injuries. Panoramic radiographs and computed tomography (CT) were evaluated by a radiologist blinded to the cases. There were persistent unremodeled extraction socket even several months after tooth extraction in 3 of the cases that were consistent wit CT findings that also showed areas of osteosclerosis and osteolysis. Patients were treated according to the recommendations of the AAOMS, with surgical debridement and antibiotic coverage with amoxicillin in the symptomatic patients. The follow-up of these patients ranged from 8 to 34 months, with a good response to treatment. The image findings in this case series were not specific and showed no difference between each stages of BRONJ (AAOMS, 2009). The image features were similar in presence or absence of exposed bone.
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Microtensile bond strength of resin cements to caries-affected dentin.
J Prosthet Dent
PUBLISHED: 07-16-2013
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The bonding of resin materials to caries-affected dentin, especially self-adhesive cements, remains a challenge in dentistry.
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Mineral loss and color change of enamel after bleaching and staining solutions combination.
J Biomed Opt
PUBLISHED: 06-04-2013
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Pigments of food and beverages could affect dental bleaching efficacy. The aim of this investigation was to evaluate color change and mineral loss of tooth enamel as well as the influence of staining solutions normally used by adolescent patients undergoing home bleaching. Initial hardness and baseline color were measured on enamel blocks. Specimens were divided into five groups (n=5): G1 (control) specimens were kept in artificial saliva throughout the experiment (3 weeks); G2 enamel was exposed to 10% carbamide peroxide for 6 h daily, and after this period, the teeth were cleaned and stored in artificial saliva until the next bleaching session; and G3, G4, and G5 received the same treatments as G2, but after bleaching, they were stored for 1 h in cola soft drink, melted chocolate, or red wine, respectively. Mineral loss was obtained by the percentage of hardness reduction, and color change was determined by the difference between the data obtained before and after treatments. Data were subjected to analysis of variance and Fishers test (?=0.05). G3 and G5 showed higher mineral loss (92.96 ± 5.50 and 94.46 ± 1.00, respectively) compared to the other groups (p ? 0.05). G5 showed high-color change (9.34 ± 2.90), whereas G1 presented lower color change (2.22 ± 0.44) (p ? 0.05). Acidic drinks cause mineral loss of the enamel, which could modify the surface and reduce staining resistance after bleaching.
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Experimental results of underwater cooperative source localization using a single acoustic vector sensor.
Sensors (Basel)
PUBLISHED: 05-07-2013
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This paper aims at estimating the azimuth, range and depth of a cooperative broadband acoustic source with a single vector sensor in a multipath underwater environment, where the received signal is assumed to be a linear combination of echoes of the source emitted waveform. A vector sensor is a device that measures the scalar acoustic pressure field and the vectorial acoustic particle velocity field at a single location in space. The amplitudes of the echoes in the vector sensor components allow one to determine their azimuth and elevation. Assuming that the environmental conditions of the channel are known, source range and depth are obtained from the estimates of elevation and relative time delays of the different echoes using a ray-based backpropagation algorithm. The proposed method is tested using simulated data and is further applied to experimental data from the Makai05 experiment, where 8-14 kHz chirp signals were acquired by a vector sensor array. It is shown that for short ranges, the position of the source is estimated in agreement with the geometry of the experiment. The method is low computational demanding, thus well-suited to be used in mobile and light platforms, where space and power requirements are limited.
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Can the cure time of endodontic sealers affect bond strength to root dentin?
Braz Dent J
PUBLISHED: 04-30-2013
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The cure time of endodontic sealers may influence the bond strength of fiber posts to root dentin. Forty teeth were selected and endodontically filled using calcium hydroxide cement and then divided into 2 groups according to the time elapsed between endodontic filling and post luting (n = 20): Immediately - glass fiber post luting immediately after endodontic filling; and Delayed - post luting performed 7 days after endodontic filling. The roots were also subdivided according to resin cement used for post luting (RelyX ARC and RelyX Unicem). The specimens were stored at 37°C for 24 h and sectioned in six 1-mm-thick slices from cervical, middle and apical thirds. The slice specimens were submitted to a push-out test at a crosshead speed of 0.5 mm/min, and the bond strength values obtained (MPa) were submitted to two-way ANOVA in a split-plot arrangement and Tukeys test (?=0.05). For both RelyX ARC and Unicem, the bond strength was significantly higher when the posts were cemented 7 days after the endodontic treatment. RelyX Unicem showed significantly higher bond strength values than RelyX ARC for both cementation periods. It was concluded that post luting should be made after the complete setting of the root canal sealer. Self-adhesive resin cement should be preferred for fiber post luting.
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Instrumented hip implants: electric supply systems.
J Biomech
PUBLISHED: 04-18-2013
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Instrumented hip implants were proposed as a method to monitor and predict the biomechanical and thermal environment surrounding such implants. Nowadays, they are being developed as active implants with the ability to prevent failures by loosening. The generation of electric energy to power active mechanisms of instrumented hip implants remains a question. Instrumented implants cannot be implemented without effective electric power systems. This paper surveys the power supply systems of seventeen implant architectures already implanted in-vivo, namely from instrumented hip joint replacements and instrumented fracture stabilizers. Only inductive power links and batteries were used in-vivo to power the implants. The energy harvesting systems, which were already designed to power instrumented hip implants, were also analyzed focusing their potential to overcome the disadvantages of both inductive-based and battery-based power supply systems. From comparative and critical analyses of the methods to power instrumented implants, one can conclude that: inductive powering and batteries constrain the full operation of instrumented implants; motion-driven electromagnetic energy harvesting is a promising method to power instrumented passive and active hip implants.
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Impact of diabetes mellitus on arterial stiffness in a representative sample of an urban Brazilian population.
Diabetol Metab Syndr
PUBLISHED: 03-15-2013
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Independent of other cardiovascular (CV) risk factors, increased arterial stiffness has been established as a predictor of morbidity and mortality. The main aim of this study was to investigate the impact of diabetes on arterial stiffness in a representative sample of an urban Brazilian population plus Amerindians.
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Sexual dysfunction predicts depression among women on hemodialysis.
Int Urol Nephrol
PUBLISHED: 03-15-2013
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Depression is common in patients on hemodialysis (HD), and its treatment fails in many cases. Among women on HD, sexual dysfunction (SD) can be an underestimated cause of depression and an impending factor for successful therapy. We aimed to evaluate the association between SD and depression among end-stage renal disease (ESRD) women undergoing HD and to test SD as an independent predictor of depression.
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Clinical trial evaluating color change and tooth sensitivity throughout and following in-office bleaching.
Int J Periodontics Restorative Dent
PUBLISHED: 03-14-2013
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The aim of this study was to assess the color alteration and sensitivity of teeth throughout and following in-office bleaching. Twenty-two volunteers participated in this clinical trial of bleaching treatment (35% hydrogen peroxide bleaching gel and placebo) applied on maxillary incisors and canines. According to a split-mouth design, the volunteers maxillary hemi-arches received either the bleaching or placebo agent, applied four times, at 1-week intervals. Color alteration and tooth sensitivity were assessed throughout and following bleaching. Statistical calculations were performed using gamma distribution and repeated-measures ANOVA. There was a statistically significant difference between teeth submitted to a bleaching agent and placebo (P < .001). At the end of the first, second, third, and fourth sessions, the bleached teeth presented color scores statistically lower than those observed immediately before bleaching. There was no difference in the color scale scores of the bleached teeth between bleaching sessions. The sensitivity data test showed a significant difference among treatments (P < .0001). Color alteration and dental sensitivity were altered by the bleaching agent.
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Influence of Abutment Type and Esthetic Veneering on Preload Maintenance of Abutment Screw of Implant-Supported Crowns.
J Prosthodont
PUBLISHED: 03-05-2013
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PURPOSE: The effect of veneering materials on screw joint stability remains inconclusive. Thus, this study evaluated the preload maintenance of abutment screws of single crowns fabricated with different abutments and veneering materials. MATERIALS AND METHODS: Sixty crowns were divided into five groups (n = 12): UCLA abutment in gold alloy with ceramic (group GC) and resin (group GR) veneering, UCLA abutment in titanium with ceramic (group TiC) and resin (group TiR) veneering, and zirconia abutment with ceramic veneering (group ZiC). Abutment screws made of gold were used with a 35 Ncm insertion torque. Detorque measurements were obtained initially and after mechanical cycling. Data were analyzed by ANOVA and Fishers exact test at a significance level of 5%. RESULTS: For the initial detorque means (in Ncm), group TiC (21.4 ± 1.78) exhibited statistically lower torque maintenance than groups GC (23.9 ± 0.91), GR (24.1 ± 1.34), and TiR (23.2 ± 1.33) (p < 0.05, Fishers exact test). Group ZiC (21.9 ± 2.68) exhibited significantly lower torque maintenance than groups GC, GR, and TiR (p < 0.05, Fishers exact test). After mechanical cycling, there was a statistically significant difference between groups TiC (22.1 ± 1.86) and GR (23.8 ± 1.56); between groups ZiC (21.7 ± 2.02) and GR; and also between groups ZiC and TiR (23.6 ± 1.30) (p < 0.05, Fishers exact test). CONCLUSIONS: Detorque reduction occurred regardless of abutment type and veneering material. More irregular surfaces in the hexagon area of the castable abutments were observed. The superiority of any veneering material concerning preload maintenance was not established.
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Effect of muscle fatigue and physical activity level in motor control of the gait of young adults.
Gait Posture
PUBLISHED: 03-05-2013
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The aim of this study was to analyze the effect of muscle fatigue in active and inactive young adults on the kinematic and kinetic parameters of normal gait and obstacle crossing. Twenty male subjects were divided into active (10) and inactive (10), based on self-reported physical activity. Participants performed three trials of two tasks (normal gait and obstacle crossing) before and after a fatigue protocol, consisting of repeated sit-to-stand transfers until the instructed pace could no longer be maintained. MANOVAs were used to compare dependent variables with the following factors: physical activity level, fatigue and task. The endurance time in the fatigue protocol was lower for the inactive group. Changes of gait parameters with fatigue, among which increased step width and increased stride speed were the most consistent, were independent of task and physical activity level. These findings indicate that the kinematic and kinetic parameters of gait are affected by muscle fatigue irrespective of the physical activity level of the subjects and type of gait. Inactive individuals used a slightly different strategy than active individuals when crossing an obstacle, independently of muscle fatigue.
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Impact of tongue biofilm removal on mechanically ventilated patients.
Rev Bras Ter Intensiva
PUBLISHED: 02-28-2013
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To evaluate the effectiveness of a tongue cleaner in the removal of tongue biofilm in mechanically ventilated patients.
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Invasive candidiasis and oral manifestations in premature newborns.
Einstein (Sao Paulo)
PUBLISHED: 02-18-2013
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To investigate prevalence of invasive candidiasis in a Neonatal Intensive Care Unit and to evaluate oral diseases and Candida spp. colonization in low birth weight preterm newborns.
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Alterations in the peripheral blood B cell subpopulations of multidrug-resistant tuberculosis patients.
Clin. Exp. Med.
PUBLISHED: 02-09-2013
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The function of B cells in the immune response against Mycobacterium tuberculosis (Mtb) is still regarded as secondary, although major findings in mouse models of tuberculosis (TB) support their participation as regulators and antibody producers. However, studies in cohorts of TB or multidrug-resistant TB (MDR-TB) patients have failed to clearly identify changes in the circulating B cell pool. Therefore, in the present study we aimed at identifying alterations in the different B cell subpopulations in peripheral blood samples of HIV-negative pulmonary MDR-TB patients when compared to healthy donors. The data show, for the first time, that MDR-TB patients, similarly to what has been observed in other chronic inflammatory diseases, have a much lower frequency of peripheral blood unswitched IgD(+)CD27(+) memory B cells. Equally novel are the findings that in MDR-TB patients there is a reduction in the circulating plasma cell pool and that in MDR-TB there is an increased frequency of circulating type 1 transitional IgD(+)CD38(++), CD69(+) and TLR9(+) B cells. These results document disease-related shifts in peripheral blood B cell subsets in MDR-TB and suggest that such changes should be taken into account when designing new strategies to boost the cellular and humoral immune response against Mtb.
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Efficacy of HPA Lanolin® in treatment of lip alterations related to chemotherapy.
J Appl Oral Sci
PUBLISHED: 02-06-2013
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The side effects of chemotherapy on the lips may cause esthetic and functional impact and increase the risk of infection. HPA Lanolin® is an option for supportive therapy because it has anti-inflammatory, antimicrobial and moisturizing properties.
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Influence of ferrule, post system, and length on biomechanical behavior of endodontically treated anterior teeth.
J Endod
PUBLISHED: 02-05-2013
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The aim of this study was to evaluate the influence of post system, length, and ferrule on biomechanical behavior of endodontically treated anterior teeth.
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