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Find video protocols related to scientific articles indexed in Pubmed.
Histological and developmental study of prehierarchical follicles in geese.
Folia Biol. (Krakow)
PUBLISHED: 11-19-2014
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The development of the follicular wall and apoptosis of corresponding cells are dependent upon the stage of follicle growth and levels of endogenous hormones. However, the development and apoptosis of prehierarchical follicles in geese is insufficiently known. In order to obtain an understanding about the microstructure, development and apoptosis of prehierarchical follicles in geese, firstly, a histological method was used to investigate the morphological structure of prehierarchical follicles. Results showed that the thickness of granulosa cell layers of the follicular wall increased first, then decreased to the lowest when follicles grew to 9-10 mm in diameter, and the theca layers also thinned to the lowest thickness at the same stage. Moreover, the expression of follicle-stimulating hormone receptor (FSHR) mRNA and the enzyme activity of caspase-3 were analyzed and the results showed that the expression of FSHR was highest when follicles grew to 8-9 mm in diameter (p < 0.05); the enzyme activity of caspae-3 was the highest when follicles grew to 6-8 mm in diameter (p < 0.05). These collective findings suggested that follicles 6-10 mm in diameter were especially significant, and perhaps represent a turning point from growing follicles to dominant follicles to be selected into a hierarchical sequence or to other follicles to be degenerated during prehierarchical follicle development.
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Preparation of 5-fluorouracil-loaded Nanoparticles and Study of Interaction with Gastric Cancer Cells.
Asian Pac. J. Cancer Prev.
PUBLISHED: 10-09-2014
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To prepare 5-fluorouracil (5-Fu) nanoparticles with higher encapsulation efficiency and drug loading, and then investigate interaction with the SGC-7901 gastric cancer cell line.
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Fast in situ generated ?-polylysine-poly (ethylene glycol) hydrogels as tissue adhesives and hemostatic materials using an enzyme-catalyzed method.
J Biomater Appl
PUBLISHED: 10-05-2014
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In this study, novel bio-inspired in situ hydrogels as tissue adhesives and hemostatic materials were designed and prepared based on ?-polylysine-grafted poly(ethylene glycol) and tyramine via enzymatic cross-linking. The enzymatic cross-linked method enabled fast gelation within seconds, which facilitated its therapeutic applications. By changing the cross-linking conditions, the storage modulus of the hydrogels could be tunable and the mechanical strength influenced the tissue adhesiveness of the hydrogels. Besides, the hydrogels showed fine network structures with appropriate pore sizes, which were thought to be a contributing factor to the strong adhesiveness. Benefiting from the strong mechanical properties and fine network structures, the ?-polylysine-grafted poly(ethylene glycol) and tyramine hydrogels exhibited superior wound-healing and hemostatic ability compared to conventional and commercially available medical materials. Moreover, indirect cytotoxicity assessment indicated that the ?-polylysine-grafted poly(ethylene glycol) and tyramine hydrogels were nontoxic to the L929 cell. These results demonstrated that the enzymatic cross-linked in situ ?-polylysine hydrogels hold high potential for tissue sealants and hemostatic materials.
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Predicted roles of defects on band offsets and energetics at CIGS (Cu(In,Ga)Se?/CdS) solar cell interfaces and implications for improving performance.
J Chem Phys
PUBLISHED: 09-08-2014
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The laboratory performance of CIGS (Cu(In,Ga)Se2) based solar cells (20.8% efficiency) makes them promising candidate photovoltaic devices. However, there remains little understanding of how defects at the CIGS/CdS interface affect the band offsets and interfacial energies, and hence the performance of manufactured devices. To determine these relationships, we use density functional theory with the B3PW91 hybrid functional that we validate to provide very accurate descriptions of the band gaps and band offsets. This confirms the weak dependence of band offsets on surface orientation observed experimentally. We predict that the conduction band offset (CBO) of perfect CuInSe2/CdS interface is large, 0.79 eV, which would dramatically degrade performance. Moreover we show that band gap widening induced by Ga adjusts only the valence band offset, and we find that Cd impurities do not significantly affect the CBO. Thus we show that Cu vacancies at the interface play the key role in enabling the tunability of CBO. We predict that Na further improves the CBO through electrostatically elevating the valence levels to decrease the CBO, explaining the observed essential role of Na for high performance. Moreover we find that K leads to a dramatic decrease in the CBO to 0.05 eV, much better than Na. We suggest that the efficiency of CIGS devices might be improved substantially by tuning the ratio of Na to K, with the improved phase stability of Na balancing phase instability from K. All these defects reduce interfacial stability slightly, but not significantly.
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Hydrogen peroxide primes heart regeneration with a derepression mechanism.
Cell Res.
PUBLISHED: 08-15-2014
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While the adult human heart has very limited regenerative potential, the adult zebrafish heart can fully regenerate after 20% ventricular resection. Although previous reports suggest that developmental signaling pathways such as FGF and PDGF are reused in adult heart regeneration, the underlying intracellular mechanisms remain largely unknown. Here we show that H2O2 acts as a novel epicardial and myocardial signal to prime the heart for regeneration in adult zebrafish. Live imaging of intact hearts revealed highly localized H2O2 (~30 ?M) production in the epicardium and adjacent compact myocardium at the resection site. Decreasing H2O2 formation with the Duox inhibitors diphenyleneiodonium (DPI) or apocynin, or scavenging H2O2 by catalase overexpression markedly impaired cardiac regeneration while exogenous H2O2 rescued the inhibitory effects of DPI on cardiac regeneration, indicating that H2O2 is an essential and sufficient signal in this process. Mechanistically, elevated H2O2 destabilized the redox-sensitive phosphatase Dusp6 and hence increased the phosphorylation of Erk1/2. The Dusp6 inhibitor BCI achieved similar pro-regenerative effects while transgenic overexpression of dusp6 impaired cardiac regeneration. H2O2 plays a dual role in recruiting immune cells and promoting heart regeneration through two relatively independent pathways. We conclude that H2O2 potentially generated from Duox/Nox2 promotes heart regeneration in zebrafish by unleashing MAP kinase signaling through a derepression mechanism involving Dusp6.
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Novel behaviors of multiferroic properties in Na-Doped BiFeO? nanoparticles.
Nanoscale
PUBLISHED: 08-12-2014
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Highly aliovalent Na(1+) ions were selected as the dopant to substitute Bi(3+) ions in BiFeO? (BFO) and Bi1-xNaxFeO? (x = 0, 0.01, 0.03, 0.05) nanoparticles prepared via a facile sol-gel method. Weak ferromagnetism and an obvious exchange bias (EB) phenomenon without field cooling were observed in the samples. To establish the presence of EB in the nanoparticles, training effect (TE) data were analyzed using Binek's model. Moreover, with the increase in Na(1+) content, the band gap was decreased, while interestingly, the leakage current density was significantly reduced and the smallest leakage current density (?10(-7) A cm(-2)) was observed for the 3% Na-doped BFO. The electrical conduction mechanism of samples was investigated by plotting log?J versus log?E. Oxygen vacancies decreased with the increase of Na content analyzed through X-ray photoelectron spectroscopy (XPS) measurements. To further explain the decrease of band gap and leakage current density with the increase of Na content, the interplay of oxygen vacancies and holes was analyzed and a phenomenological qualitative model based on the electronic energy band proposed.
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Spatially continuous distributed fiber optic sensing using optical carrier based microwave interferometry.
Opt Express
PUBLISHED: 08-05-2014
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This paper reports a spatially continuous distributed fiber optic sensing technique using optical carrier based microwave interferometry (OCMI), in which many optical interferometers with the same or different optical path differences are interrogated in the microwave domain and their locations can be unambiguously determined. The concept is demonstrated using cascaded weak optical reflectors along a single optical fiber, where any two arbitrary reflectors are paired to define a low-finesse Fabry-Perot interferometer. While spatially continuous (i.e., no dark zone), fully distributed strain measurement was used as an example to demonstrate the capability, the proposed concept may also be implemented on other types of waveguide or free-space interferometers and used for distributed measurement of various physical, chemical and biological quantities.
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[Case-control study on the modified ilioinguinal incision of anterior approach for the treatment of pelvic and acetabular fractures].
Zhongguo Gu Shang
PUBLISHED: 07-18-2014
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To study modified ilioinguinal approach through the retrospective analysis on the surgical treatment of 63 patients with pelvic and acetabular fractures through anterior approach.
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[Rapid determination of ractopamine content in pork by using three-dimensional synchronous fluorescence spectrum coupled with APTLD].
Guang Pu Xue Yu Guang Pu Fen Xi
PUBLISHED: 07-11-2014
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In order to realize the rapid determination of ractopamine content in pork, quantitative determination model of ractopamine content in pork was established by using three-dimensional synchronous fluorescence spectrum coupled with alternating penalty trilinear decomposition (APTLD). Firstly, the generation mechanism of the fluorescence spectrum for ractopamine and three-dimensional synchronous fluorescence spectrum for samples were analyzed. Secondly, concentration quenching phenomenon of fluorescence of ractopamine in pork extract was investigated. Thirdly, the number of components for three linear decomposition of APTLD was set as 2 by using the core consistency diagnostic method, and the calibration curve of the relative fluorescence intensity of ractopamine between pork extract and the training sample was established for the correction of relative fluorescence intensity of prediction samples. Finally, three-dimensional synchronous fluorescence spectrum combined with APTLD was used to build the prediction model of ractopamine content in pork. The experimental results showed that the method adopted in the paper could better solve the problem of serious synchronous fluorescence spectrum overlapping between ractopamine in pork samples and backgrounds, and leave out some trivial process of chemical separation for the identification of ractopamine in pork. The determination coefficient (R2) and the root mean squared error of prediction (RMSEP) for the model proposed in this paper were 0.986 3 and 0.496 6 mg x L(-1), respectively. The method in this paper has achieved the goal of rapid quantitative detection of ractopamine content in pork.
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Synthesis and evaluation of bisbenzylidenedioxotetrahydrothiopranones as activators of endoplasmic reticulum (ER) stress signaling pathways and apoptotic cell death in acute promyelocytic leukemic cells.
J. Med. Chem.
PUBLISHED: 07-11-2014
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Curcumin is known to trigger ER-stress induced cell death of acute promyelocytic leukemic (APL) cells by intercepting the degradation of nuclear co-repressor (N-CoR) protein which has a key role in the pathogenesis of APL. Replacing the heptadienedione moiety of curcumin with a monocarbonyl cross-conjugated dienone embedded in a tetrahydrothiopyranone dioxide ring resulted in thiopyranone dioxides that were more resilient to hydrolysis and had greater growth inhibitory activities than curcumin on APL cells. Several members intercepted the degradation of misfolded N-CoR and triggered the signaling cascade in the unfolded protein response (UPR) which led to apoptotic cell death. Microarray analysis showed that genes involved in protein processing pathways that were germane to the activation of the UPR were preferentially up-regulated in treated APL cells, supporting the notion that the UPR was a consequential mechanistic pathway affected by thiopyranone dioxides. The Michael acceptor reactivity of the scaffold may have a role in exacerbating ER stress in APL cells.
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All-in-fiber optofluidic sensor fabricated by femtosecond laser assisted chemical etching.
Opt Lett
PUBLISHED: 07-01-2014
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An all-in-fiber prototype optofluidic device was fabricated by femtosecond laser irradiation and subsequent selective chemical wet etching. Horizontal and vertical microchannels can be flexibly created into an optical fiber to form a fluidic cavity with inlets/outlets. The fluidic cavity also functions as an optical Fabry-Perot cavity in which the filled liquid can be probed. The assembly-free microdevice exhibited a fringe visibility of 20 dB and was demonstrated for measurement of the refractive index of the filling liquids. The proposed all-in-fiber optofluidic micro device is attractive for chemical and biomedical sensing because it is flexible in design, simple to fabricate, mechanically robust, and miniaturized in size.
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Control of critical coupling in a coiled coaxial cable resonator.
Rev Sci Instrum
PUBLISHED: 06-02-2014
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This paper reports a coiled coaxial cable resonator fabricated by cutting a slot in a spring-like coiled coaxial cable to produce a periodic perturbation. Electromagnetic coupling between two neighboring slots was observed. By manipulating the number of slots, critical coupling of the coiled coaxial cable resonator can be well controlled. An ultrahigh signal-to-noise ratio (over 50 dB) at the resonant frequency band was experimentally achieved from a coiled coaxial cable resonator with 38 turns. A theoretic model is developed to understand the device physics. The proposed device can be potentially used as a high quality and flexibly designed band-stop filter or a sensor in structural health monitoring.
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[Pathological changes of after trans-portal vein chemoembolization Echinococcus multilocularis in the liver of infected rats].
Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi
PUBLISHED: 05-15-2014
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To observe the morphological change in the pathological process of Echinococcus multilocularis in liver of infected rats after treatment with liposome entrapped albendazole and iodized oil suspension through portal vein, and investigate its efficacy against hepatic alveolar echinococcosis.
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[Treatment of pituitary adenomas invading cavernous sinus with neuroendoscopy assisted by multiple techniques].
Zhongguo Yi Xue Ke Xue Yuan Xue Bao
PUBLISHED: 05-06-2014
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To study the effectiveness of treating giant pituitary adenomas invading cavernous sinus with neuroendoscopy assisted by multiple techniques.
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Anti-inflammatory and anti-apoptotic effect of combined treatment with methylprednisolone and amniotic membrane mesenchymal stem cells after spinal cord injury in rats.
Neurochem. Res.
PUBLISHED: 04-15-2014
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This study was undertaken to investigate the synergistic effects of methylprednisolone (MP) administration and transplantation of amniotic membrane mesenchymal stem cells (AM-MSCs) following T11 spinal cord clip compressive injury in rats. The combination treatment with MP (50 mg/kg) and delayed transplantation of AM-MSCs after rat spinal cord injury, significantly reduced (1) myeloperoxidase activity, (2) the proinflammatory cytokines: tumor necrosis factor-?, interleukin (IL)-1?, IL-6, IL-17, interferon-? and (3) the cell apoptosis [terminal deoxynucleotidyl transferase, dUTP nick end labeling (TUNEL) staining, and caspase-3, Bax and Bcl-2 expressions]; increased: (1) the levels of the anti-inflammatory cytokines (IL-10 and transforming growth factor-?1) and (2) the survival rate of AM-MSCs in the injury site. The combination therapy significantly ameliorated the recovery of limb function (evaluated by Basso, Beattie and Bresnahan score). Taken together, our results demonstrate that MP in combination with AM-MSCs transplantation is a potential strategy for reducing secondary damage and promoting functional recovery following spinal cord injury.
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DNA methylation study of fetus genome through a genome-wide analysis.
BMC Med Genomics
PUBLISHED: 04-10-2014
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DNA methylation is a crucial epigenetic modification of the genome which is involved in embryonic development, transcription, chromatin structure, X chromosome inactivation, genomic imprinting and chromosome stability. Consistent with these important roles, DNA methylation has been demonstrated to be required for vertebrate early embryogenesis and essential for regulating temporal and spatial expression of genes controlling cell fate and differentiation. Further studies have shown that abnormal DNA methylation is associated with human diseases including the embryonic development diseases. We attempt to study the DNA methylation status of CpG islands in fetus related to fetus growth and development.
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Cyclooxygenase-2 inhibition as a strategy for treating gastric adenocarcinoma.
Oncol. Rep.
PUBLISHED: 04-04-2014
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Cyclooxygenase-2 (COX-2) has been proven to play critical roles in inflammation as well as in cancer. Some studies have shown that the anti-inflammatory, immunosuppressive and anti-arthritic effects of celecoxib are mainly attributed to the inhibition of COX-2 expression. The present study aimed to investigate the function of COX-2 in human gastric adenocarcinoma (GAC). Forty-five cases of human GAC tissues and corresponding adjacent non-cancerous tissues (ANCTs) were collected. The expression of COX-2 and proliferating cell nuclear antigen (PCNA) was assessed using immunohistochemical assay through a tissue microarray procedure. GAC cells (SGC-7901 and MKN-45) in vitro were treated with COX-2 siRNA or different concentrations of celecoxib to observe their effects on cell proliferation, invasion and the underlying molecular mechanisms. As a consequence, the expression of COX-2 and PCNA was found in cancer tissues with a higher strong reactivity rate, compared with the ANCTs (80.0 vs. 53.3%, P=0.011; 68.9 vs. 48.9%, P=0.047), and COX-2 was positively associated with lymph node metastasis of GAC patients (P=0.011). Targeted knockdown of COX-2 inhibited the proliferation, migration and invasion of GAC cells with decreased expression of PCNA. COX-2 inhibitor celecoxib also suppressed the proliferative activities of GAC cells with decreased expression of COX-2 and PCNA. In addition, the tumor volume in the MKN-45 subcutaneous tumor model treated with siCOX-2 was significantly smaller than that of the negative control (NC) group (P<0.01). Taken together, our findings offer a strong preclinical rationale to target COX-2 signaling as a therapeutic strategy to improve the treatment of gastric adenocarcinoma.
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The effect of postural correction and subsequent balloon inflation in deformity correction of acute osteoporotic vertebral fractures.
J Korean Neurosurg Soc
PUBLISHED: 03-29-2014
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To determine deformity correction by postural correction and subsequent balloon inflation in acute vertebral compression fractures (OVCFs) and to examine the effect of bone mineral density on deformity correction.
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Extraesophageal saline enhances endoscopic ultrasonography to differentiate esophagus and adjacent organs.
World J. Gastroenterol.
PUBLISHED: 03-03-2014
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To distinguish between the esophagus and adjacent organs using extraesophageal saline injection (ESI) in a canine model.
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Reflection based Extraordinary Optical Transmission Fiber Optic Probe for Refractive Index Sensing.
Sens Actuators B Chem
PUBLISHED: 02-28-2014
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Fiber optic probes for chemical sensing based on the extraordinary optical transmission (EOT) phenomenon are designed and fabricated by perforating subwavelength hole arrays on the gold film coated optical fiber endface. The device exhibits a red shift in response to the surrounding refractive index increases with high sensitivity, enabling a reflection-based refractive index sensor with a compact and simple configuration. By choosing the period of hole arrays, the sensor can be designed to operate in the near infrared telecommunication wavelength range, where the abundant source and detectors are available for easy instrumentation. The new sensor probe is demonstrated for refractive index measurement using refractive index matching fluids. The sensitivity reaches 573 nm/RIU in the 1.333~1.430 refractive index range.
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Prenatal nicotinic exposure suppresses fetal adrenal steroidogenesis via steroidogenic factor 1 (SF-1) deacetylation.
Toxicol. Appl. Pharmacol.
PUBLISHED: 02-26-2014
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This study aimed to investigate the suppressive effect of nicotine on fetal adrenal steroidogenesis and to explore the potential role of epigenetic modification of steroidogenic factor-1 (SF-1) transcriptional activity in this process. Nicotine was intragastrically administered to pregnant rats and NCI-H295A cells were treated with nicotine or trichostatin A (TSA). The pathomorphology of fetal adrenals, steroid hormone levels, the expression of SF-1 and its target genes, and histone deacetylase (HDAC) mRNA were analyzed. Histone modification and DNA methylation of the SF-1 promoter region were assessed using chromatin immunoprecipitation (ChIP) and bisulfite sequencing PCR. The interaction between SF1 and its target genes was observed. Prenatal nicotinic exposure decreased fetal body weight, increased the IUGR rate and caused detrimental changes in fetal adrenal. In addition, the levels of corticosterone, the expression of SF-1 and its target genes were decreased while HDAC2 expression was enhanced. Nicotine treatment decreased histone H3K9 and H3K14 acetylation levels while there was no effect on the methylation frequency on the SF-1 promoter region. Furthermore, in nicotine-treated NCI-H295A cells, lower levels of steroidogenic synthesis, lower expression of SF-1 and its target genes were observed while the expression of HDACs was enhanced. The interaction between SF1 and StAR decreased with nicotine treatment. Nicotine treatment decreased histone H3K9 and H3K14 acetylation levels, and addition of TSA reversed the inhibition of nicotine-mediated SF-1 and its partial target genes. Thus, nicotine-mediated reduction of SF-1 expression resulted in an inhibitory effect on the expression of its target genes and steroid production via histone deacetylation.
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Ursolic acid suppresses growth and adrenocorticotrophic hormone secretion in AtT20 cells as a potential agent targeting adrenocorticotrophic hormone-producing pituitary adenoma.
Mol Med Rep
PUBLISHED: 02-26-2014
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Adrenocorticotrophic hormone (ACTH)-producing pituitary adenoma leads to excess ACTH secretion, which is associated with significant mortality and impaired quality of life. Thus far, the first line therapy is the transphenoidal microsurgery. Considering the high recurrence rate and complications of surgery, novel agents, which directly target on pituitary ACTH-producing adenoma and suppress ACTH secretion are urgently required. In the present study, the effect of ursolic acid (UA) as a candidate agent targeting ACTH-producing AtT20 cells was investigated. It was demonstrated that UA inhibited the viability and induced apoptosis of AtT20 cells and decreased ACTH secretion. The process of apoptosis involved a decrease of the B cell lymphoma 2 (Bcl-2)/Bcl2-associated X protein ratio followed by a release of mitochondrial cytochrome c into the cytosol with subsequent activation of caspase-9, -3/7 and -8. The potential signaling pathway involved the activation of c-Jun N-terminal kinase (JNK) but not extracellular signal-regulated protein kinases1/2 and p38 mitogen-activated protein kinase. The JNK pathway participated in UA-induced mitochondrial apoptotic signaling transduction via increasing the phosphorylation and degradation of Bcl-2, which may be partly attenuated by the JNK inhibitor SP600125. In conclusion, the present study indicates that UA may be a promising candidate agent for the management of ACTH-producing pituitary adenoma.
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Coaxial cable Bragg grating assisted microwave coupler.
Rev Sci Instrum
PUBLISHED: 02-13-2014
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This paper reports a microwave coupler based on two parallel coaxial cable Bragg gratings fabricated by drilling U-grooves across the cables at periodic distance along the cable direction. Electromagnetic field couplings between two cables were observed at discrete frequencies through both near and far ends detections. The coupling frequency and strength can be precisely controlled by varying the grating period and length. The coupling bandwidth may also be controlled through specific grating design. The device physics was also described through transfer matrix which matched well with the experimental results.
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A comparison of the life-history traits between diapause and direct development individuals in the cotton bollworm, Helicoverpa armigera.
J. Insect Sci.
PUBLISHED: 02-05-2014
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In order to understand the differences of life-history traits between diapause and direct development individuals in the cotton bollworm, Helicoverpa armigera (Hübner) (Lepidoptera: Noctuidae), the development time, body size, growth rate, and adult longevity were investigated between the two populations, which were induced under 12:12 L:D and 16:8 L:D photoperiods, respectively, at 20, 22, and 25°C. The results indicated that the larval development time, pupal weight, adult weight, and growth rate were significantly different between diapause and direct developing individuals. The diapause developing individuals had a significantly higher pupal and adult weight and a longer larval time compared with direct developing individuals. However, the growth rate in diapause developing individuals was lower than that in the direct developing individuals. Analysis by GLM showed that larval time, pupal and adult weight, and growth rate were significantly influenced by both temperature and developmental pathway. The pupal and adult weights were greater in males than females in both developmental pathways, exhibiting sexual size dimorphism. The dimorphism in adult weight was more pronounced than in pupal weight because female pupae lost more weight at metamorphosis compared to male pupae. Protogyny was observed in both developmental pathways. However, the protogyny phenomenon was more pronounced at lower temperatures in direct developing individuals, whereas it was more pronounced in diapause developing individuals when they experienced higher temperatures in their larval stage and partial pupal period. The adult longevity of diapause developing individuals was significantly longer than that of direct developing individuals. The results reveal that the life-history strategy was different between diapause and direct developing individuals.
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Gene cloning, expression, and characterization of an exo-inulinase from Paenibacillus polymyxa ZJ-9.
Appl. Biochem. Biotechnol.
PUBLISHED: 01-12-2014
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An inulinase-producing strain, Paenibacillus polymyxa ZJ-9, was isolated from natural sources to produce R,R-2,3-butanediol via one-step fermentation of raw inulin extracted from Jerusalem artichoke tubers. The inulinase gene from P. polymyxa ZJ-9 was cloned and overexpressed in Escherichia coli BL21 (DE3), and the purified recombinant inulinase was estimated to be approximately 56 kDa by both sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and gel filtration chromatography. This result suggests that the active form of the inulinase is probably a monomer. Terminal hydrolysis fructose units from the inulin indicate that enzymes are exo-inulinase. The purified recombinant enzyme showed maximum activity at 25 °C and pH 6.0, which indicate its extreme suitability for industrial applications. Zn(2+), Fe(2+), and Mg(2+) stimulated the activity of the purified enzyme, whereas Co(2+), Cu(2+), and Ni(2+) inhibited enzyme activity. The K m and V max values for inulin hydrolysis were 1.72 mM and 21.69 ?mol min(-1) mg(-1) protein, respectively. The same parameters toward sucrose were 41.09 mM and 78.7 ?mol min(-1) mg(-1) protein, respectively. Considering its substrate specificity and other enzymatic characteristics, we believe that this inulinase gene from P. polymyxa ZJ-9 could be transformed into other special bacterial strains to allow inulin conversion to other biochemicals and bioenergy through one-step fermentation.
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MDR1 gene polymorphisms are associated with glucocorticoid-induced avascular necrosis of the femoral head in a Chinese population.
Genet Test Mol Biomarkers
PUBLISHED: 01-04-2014
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P-glycoprotein (P-gp) is present in various tissue cells, required for the pumping of lipophilic drugs (including glucocorticoids) out of cells. We hypothesized that polymorphisms in the P-gp encoding gene (multidrug-resistant transporter-1 [MDR1]) are related to individual differences in glucocorticoid sensitivity and the development of glucocorticoid-induced avascular necrosis of the femoral head (GANFH). In this case-control study, we genotyped three known single-nucleotide polymorphisms (SNPs: C1236T, G2677T/A, and C3435T) within the MDR1 gene in 662 Chinese subjects. Statistically significant differences between GANFH patients and either healthy controls or glucocorticoid-resistant patients (non-GANFH) were found for the T allele or TT genotype of C3435T. The haplotype TTT, composed of these three SNPs, exhibited a significant association with the disease. No associations were identified between C1236T or G2677T/A and GANFH. Our results suggest that the C3435T polymorphism of the MDR1 gene is associated with susceptibility to GANFH in a Chinese population.
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Correlations of pituitary tumor transforming gene expression with human pituitary adenomas: a meta-analysis.
PLoS ONE
PUBLISHED: 01-01-2014
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Pituitary tumor transforming gene (PTTG) is an important paracrine growth factor involved in early lactotrope transformation and early onset of angiogenesis in pituitary hyperplasia. Emerging evidences have shown that PTTG expression may contribute to the etiology of pituitary adenomas; but individually published studies showed inconclusive results. This meta-analysis aimed to derive a more precise estimation of the correlations of PTTG expression with human pituitary adenomas.
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[Significance of pseudocapsule in the excision of pituitary adenomas in transsphenoidal surgery].
Zhonghua Yi Xue Za Zhi
PUBLISHED: 12-24-2013
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To explore the significance of pseudocapsule in the excision of pituitary adenomas in transsphenoidal surgery.
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[Application of factor analysis to evaluate deformation behaviors of frequently-used tablet excipients].
Yao Xue Xue Bao
PUBLISHED: 12-24-2013
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The present work is to investigate the correlation between physical properties and deformation behaviors of tablet excipients, and rank them according to their plastic performances during compaction. The excipients selected were compacted using Korsch XP1 after measuring their physical properties where the compression parameters for evaluating deformation behaviors were Heckle equation, compression work and elastic stretch in die. The correlations between compaction descriptors and physical parameters were analyzed by canonical correlation analysis, and factor analysis was simultaneously employed to synthetically assess deformation behaviors for all our samples. The canonical variables show that true density (Pa) correlated negatively with plastic coefficient (PL) and positively with yield pressure (YP); compression degree (Cp) correlated negatively with fast elastic stretch (FES) as well as YP and positively with PL. When factor scores were used in combination with original data, the plasticity of our samples was sorted and ranked as high (-0.56 < F < 0.21), intermediate (-0.16 < F < 0.36), or low (0.38 < F < 0.84), which are in accord with plasticity rankings previously reported in literature. This study indicates factor analysis can be an approach to evaluate deformation behaviors of pharmaceutical powders.
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High-temperature fiber-optic Fabry-Perot interferometric pressure sensor fabricated by femtosecond laser.
Opt Lett
PUBLISHED: 12-11-2013
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In this Letter, we report on a fiber-optic Fabry-Perot interferometric pressure sensor with its external diaphragm surface thinned and roughened by a femtosecond laser. The laser-roughened surface helps to eliminate outer reflections from the external diaphragm surface and makes the sensor immune to variations in the ambient refractive index. The sensor is demonstrated to measure pressure in a high-temperature environment with low-temperature dependence.
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Optical fiber magnetic field sensor based on single-mode-multimode-single-mode structure and magnetic fluid.
Opt Lett
PUBLISHED: 12-11-2013
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An optical fiber magnetic field sensor based on the single-mode-multimode-single-mode (SMS) structure and magnetic fluid (MF) is proposed and demonstrated. By using a piece of no-core fiber as the multimode waveguide in the SMS structure and MF sealed in a capillary tube as the magnetic sensitive media, which totally immersing the no-core fiber, an all-fiber magnetic sensor was fabricated. Interrogation of the magnetic field strength can be achieved either by measuring the dip wavelength shift of the transmission spectrum or by detecting the transmission loss at a specific wavelength. A demonstration sensor with sensitivities up to 905??pm/mT and 0.748??dB/mT was fabricated and investigated. A theoretical model for the design of the proposed device was developed and numerical simulations were performed.
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Targeting SHCBP1 Inhibits Cell Proliferation in Human Hepatocellular Carcinoma Cells.
Asian Pac. J. Cancer Prev.
PUBLISHED: 12-03-2013
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Src homology 2 domain containing (SHC) is a proto-oncogene which mediates cell proliferation and carcinogenesis in human carcinomas. Here, the SHC SH2-domain binding protein 1 (SHCBP1) was first established to be up-regulated in human hepatocellular carcinoma (HCC) tissues by array-base comparative genome hybridization (aCGH). Meanwhile, we examine and verify it by quantitative real-time PCR and western blot. Our current data show that SHCBP1 was up-regulated in HCC tissues. Overexpression of SHCBP1 could significantly promote HCC cell proliferation, survival and colony formation in HCC cell lines. Furthermore, knockdown of SHCBP1 induced cell cycle delay and suppressed cell proliferation. Furthermore, SHCBP1 could regulate the expression of activate extracellular signal-regulated kinase 1/2 (ERK1/2) and cyclin D1. Together, our findings indicate that SHCBP1 may contribute to human hepatocellular carcinoma by promoting cell proliferation and may serve as a molecular target of cancer therapy.
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Inhibitory effect of the gallotannin corilagin on dextran sulfate sodium-induced murine ulcerative colitis.
J. Nat. Prod.
PUBLISHED: 11-07-2013
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The therapeutic effect of corilagin (1) was evaluated in an acute colitis model induced by dextran sulfate sodium (DSS) in mice, and the mechanism of action was investigated in this study. Animals were challenged with 2% DSS drinking water for 5 consecutive days and then intraperitoneally treated with 1 (7.5, 15, and 30 mg/kg) daily for 7 days. It was found that 1 significantly decreased the disease activity index, inhibited the shortening of colon length, reduced colon tissue damage, and suppressed myeloperoxidase activity. Moreover, 1 greatly suppressed the secretion of TNF-?, IL-6, and IL-1?, inhibited the degradation of I?B ?, and down-regulated expression of cleaved caspase-3 and cleaved caspase-9 in colon tissues of DSS-treated mice. These findings demonstrated that 1 exerts a protective effect on DSS-induced colitis, and its underlying mechanisms are associated with inhibition of the NF-?B pathway that mitigates colon inflammatory responses and apoptosis of intestinal epithelial cells.
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[Evolvement and pollution of heavy metals in core sediments from Yamenqi Lake in Lianhuan Lake, China].
Huan Jing Ke Xue
PUBLISHED: 11-07-2013
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One sediment core was obtained from the center of Yamenqi Lake at the Songnen Plain in eastern China in August 2010 using a gravity corer. The sediment samples were digested using HNO3-HClO4-HF. Concentrations of the metals Cr, Cu, Mn, Ni, Fe, Pb, Zn, Cd, Al, Ba, Ca, K, Li, Mg, Na and Sr were determined using inductively coupled plasma-mass spectrometry (ICP-MS). With the dating approach, the characteristics and polluted history of heavy metals in lake sediments were determined. The heavy metal pollution of sediment was discussed based on the enrichment factor. The results are listed as following:(1) the contents of all elements had a smooth variation trend before 1950,and fluctuated severely from 1950 to 1990. The contents of Mn, Zn, Cd, Pb, Fe, Ni, Cr, Cu, Ca, Li and Sr increased obviously since 1990. (2) Mn, Zn, Pb and Cd showed light contamination degree. Contents of Ni, Cr and Cu were below the contamination level.
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Membrane permeability-guided identification of neuroprotective components from Polygonum cuspidatun.
Pharm Biol
PUBLISHED: 10-21-2013
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Abstract Context: Polygonum cuspidatum Sieb et Zucc. (Polygonaceae) possesses various pharmacological activities and has been widely using as one of the most popular and valuable Chinese herbal medicines in clinics. Its usage has increasingly attracted much of our attention and urges investigation on its bioactive components. Objective: To establish a rapid and valid approach for screening potential neuroprotective components from P. cuspidatum. Materials and methods: Potential neuroprotective components from P. cuspidatum were screened utilizing liposome equilibrium dialysis followed by high-performance liquid chromatography (HPLC) analysis. Their neuroprotective effects on modulation of protein expression of ?7 nAChR, ?3 nAChR and synaptophysin (SPY) on SH-SY5Y human neuroblastoma cell line (SH-SY5Y) were evaluated by means of Western blotting. Results: Two potential compounds, polydatin (C1) and emodin-8-O-?-d-glucoside (C2), were detected and identified in our study. The biological tests showed that both compounds C1 and C2, respectively, at concentrations of 0.1 and 0.25?mg/mL significantly increased protein expression of ?7 and ?3 nicotinic acetylcholine receptors (nAChRs) in SH-SY5Y cells. Moreover, C1 and C2 at 0.1?mg/mL significantly reversed the A?1-42-induced decrease of ?7 and ?3 nAChRs protein expression in SH-SY5Y cells. In addition, C2 at 0.1?mg/mL significantly increased protein expression of SPY in SH-SY5Y cells and A?1-42-induced SH-SY5Y cells whereas C1 did not provide any positive effects. Discussion and conclusion: In conclusion, our approach utilizing liposome equilibrium dialysis combined with HPLC analysis and cell-based assays is a prompt and useful method for screening neuroprotective agents.
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[Analysis of a hereditary protein C deficient consanguineous pedigree caused by Phe139Val homozygous mutation].
Zhonghua Xue Ye Xue Za Zhi
PUBLISHED: 10-10-2013
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To analyze genetic mutation and explore its molecular pathogenesis for an hereditary protein C(PC)deficient consanguineous pedigree.
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A coaxial cable Fabry-Perot interferometer for sensing applications.
Sensors (Basel)
PUBLISHED: 09-02-2013
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This paper reports a novel coaxial cable Fabry-Perot interferometer for sensing applications. The sensor is fabricated by drilling two holes half-way into a coaxial cable. The device physics was described. The temperature and strain responses of the sensor were tested. The measurement error was calculated and analyzed.
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Microwave assisted reconstruction of optical interferograms for distributed fiber optic sensing.
Opt Express
PUBLISHED: 08-14-2013
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This paper reports a distributed fiber optic sensing technique through microwave assisted separation and reconstruction of optical interferograms in spectrum domain. The approach involves sending a microwave-modulated optical signal through cascaded fiber optic interferometers. The microwave signal was used to resolve the position and reflectivity of each sensor along the optical fiber. By sweeping the optical wavelength and detecting the modulation signal, the optical spectrum of each sensor can be reconstructed. Three cascaded fiber optic extrinsic Fabry-Perot interferometric sensors were used to prove the concept. Their microwave-reconstructed interferogram matched well with those recorded individually using an optical spectrum analyzer. The application in distributed strain measurement has also been demonstrated.
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N-methyl-2-pyrrolidonium methyl sulfonate acidic ionic liquid as a new dynamic coating for separation of basic proteins by capillary electrophoresis.
Electrophoresis
PUBLISHED: 08-06-2013
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A simple and economical CE method has been developed for the analysis of four model basic proteins by employing N-methyl-2-pyrrolidonium methyl sulfonate ionic liquid (IL) as the dynamic coating material based on the interaction of both between electrostatic attraction and hydrogen bond, and between the organic cations of IL and the inner surface of bare fused-silica capillary. The N-methyl-2-pyrrolidonium-based IL modified capillary not only generated a stable suppressed electroosmotic flow, but also effectively eliminated the wall adsorption of proteins. Several important parameters such as the IL concentration, pH values, and concentrations of the background electrolyte were optimized to improve the separation of basic proteins. Consequently, under the optimum separation conditions, a satisfied separation of basic proteins including lysozyme, cytochrome c, ribonuclease A, and ?-chymotrypsinogen A with theoretical plates ranging from 2.09 × 10(5) to 4.48 × 10(5) plates/m had been accomplished within 15 min. The proposed method first illustrated the effect of hydrogen bond between coating material and inner capillary surface on the coating, which should be a new strategy to design and select more effective coating materials to form more stable coatings in CE.
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Asymmetric Facet Joint Osteoarthritis and its Relationships to Facet Orientation, Facet Tropism and Ligamentum Flavum Thickening.
J Spinal Disord Tech
PUBLISHED: 08-02-2013
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The degrees of osteoarthritis of the left and right facet joints were evaluated by using computerized tomography (CT) among elderly patients with low back or leg pain.
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Superficial esophageal lesions detected by endoscopic ultrasound enhanced with submucosal edema.
World J. Gastroenterol.
PUBLISHED: 08-01-2013
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To determine if there is consistency between endoscopic ultrasound (EUS) findings and pathological results for detecting lesions of different depth in the esophageal mucosa.
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Inhibition of novel reassortant avian influenza H7N9 virus infection in vitro with three antiviral drugs, oseltamivir, peramivir and favipiravir.
Antivir. Chem. Chemother.
PUBLISHED: 07-24-2013
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A novel reassortant avian-origin influenza A (H7N9) virus was isolated from respiratory specimens obtained from three patients and was identified as H7N9 in China. The antiviral agents are required to treat the patients with the avian influenza H7N9 virus infection.
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Fiber pigtailed thin wall capillary coupler for excitation of microsphere WGM resonator.
Opt Express
PUBLISHED: 07-12-2013
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In this paper, we demonstrate a fiber pigtailed thin wall capillary coupler for excitation of Whispering Gallery Modes (WGMs) of microsphere resonators. The coupler is made by fusion-splicing an optical fiber with a capillary tube and consequently etching the capillary wall to a thickness of a few microns. Light is coupled through the peripheral contact between inserted microsphere and the etched capillary wall. The coupling efficiency as a function of the wall thickness was studied experimentally. WGM resonance with a Q-factor of 1.14 × 10(4) was observed using a borosilicate glass microsphere with a diameter of 71 ?m. The coupler operates in the reflection mode and provides a robust mechanical support to the microsphere resonator. It is expected that the new coupler may find broad applications in sensors, optical filters and lasers.
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Heterocyclic glucocorticoid receptor modulators with a 2,2-dimethyl-3-phenyl-N-(thiazol or thiadiazol-2-yl)propanamide core.
Bioorg. Med. Chem. Lett.
PUBLISHED: 07-01-2013
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A series of heterocyclic glucocorticoid receptor (GR) modulators with 2,2-dimethyl-3-phenyl-N-(thiazol or thiadiazol-2-yl)propanamide core are described. Structure-activity relationships suggest a combination of H-bond acceptor and a 4-fluorophenyl moiety as being important structural components contributing to the glucocorticoid receptor binding and functional activity for this series of GR modulators.
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[Gene analysis in four inherited coagulation F? deficiency pedigree].
Zhonghua Xue Ye Xue Za Zhi
PUBLISHED: 05-21-2013
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To identify the genotype and pathogenesis in four Chinese pedigrees with Factor ? deficiency.
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Dependence on the structure and surface polarity of ZnS photocatalytic activities of water splitting: first-principles calculations.
Phys Chem Chem Phys
PUBLISHED: 05-14-2013
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It has been reported that phase structure and surface polarity largely affect the photocatalytic efficiency of semiconductor nanostructures. To understand the chemical activity of ZnS at the electronic level, we investigate electron structures and carrier transportation ability for bulk intrinsic zinc blende (ZB) and wurtzite (WZ) ZnS, as well as the reaction pathway of hydrogen generation from water splitting on Zn- and S-terminated polar surfaces. The electron structure calculations prove that the WZ phase possesses a higher reducing ability than the ZB phase. The conductivity of the bulk ZB phase surpasses that of the WZ phase at or above room temperature. As the temperature increases, the asymptotic conductivity ratio of WZ/ZB is close to the Golden Ratio, 0.62. Reaction kinetics studies indicate that Zn-terminated polar surfaces are more chemically active than S-terminated polar surfaces in the reaction of hydrogen generation from water splitting. The calculation results suggest that the first H splitting from water on Zn-terminated polar surfaces can occur with ground state electronic structures, while photo-assistance is necessary for the first H splitting on the S-terminated surfaces. Electronic triplet states calculations further show that Zn-terminated surfaces are more photosensitive than S-terminated surfaces.
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Discovery of potent and selective nonsteroidal indazolyl amide glucocorticoid receptor agonists.
Bioorg. Med. Chem. Lett.
PUBLISHED: 05-03-2013
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Modification of a phenolic lead structure based on lessons learned from increasing the potency of steroidal glucocorticoid agonists lead to the discovery of exceptionally potent, nonsteroidal, indazole GR agonists. SAR was developed to achieve good selectivity against other nuclear hormone receptors with the ultimate goal of achieving a dissociated GR agonist as measured by human in vitro assays. The specific interactions by which this class of compounds inhibits GR was elucidated by solving an X-ray co-crystal structure.
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3D-QSAR and 3D-QSSR studies of thieno[2,3-d]pyrimidin-4-yl hydrazone analogues as CDK4 inhibitors by CoMFA analysis.
Acta Pharmacol. Sin.
PUBLISHED: 04-26-2013
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Aim:To investigate the structural basis underlying potency and selectivity of a series of novel analogues of thieno[2,3-d]pyrimidin-4-yl hydrazones as cyclin-dependent kinase 4 (CDK4) inhibitors and to use this information for drug design strategies.Methods:Three-dimensional quantitative structure-activity relationship (3D-QSAR) and three-dimensional quantitative structure-selectivity relationship (3D-QSSR) models using comparative molecular field analysis (CoMFA) were conducted on a training set of 48 compounds. Partial least squares (PLS) analysis was employed. External validation was performed with a test set of 9 compounds.Results:The obtained 3D-QSAR model (q(2)=0.724, r(2)=0.965, r(2)pred=0.945) and 3D-QSSR model (q(2)=0.742, r(2)=0.923, r(2)pred=0.863) were robust and predictive. Contour maps with good compatibility to active binding sites provided insight into the potentially important structural features required to enhance activity and selectivity. The contour maps indicated that bulky groups at R1 position could potentially enhance CDK4 inhibitory activity, whereas bulky groups at R3 position have the opposite effect. Appropriate incorporation of bulky electropositive groups at R4 position is favorable and could improve both potency and selectivity to CDK4.Conclusion:These two models provide useful information to guide drug design strategies aimed at obtaining potent and selective CDK4 inhibitors.
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[Analysis of a consanguineous pedigree featuring hereditary coagulation factor ? deficiency].
Zhonghua Yi Xue Yi Chuan Xue Za Zhi
PUBLISHED: 04-10-2013
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To screen potential mutation and explore the underlying mechanism for a consanguineous pedigree featuring hereditary coagulation factor ? (F?) deficiency.
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Perinatal complications and higher risks of offspring thyroid dysfunction in early childhood of Graves disease mothers with euthyroidism.
J. Pediatr. Endocrinol. Metab.
PUBLISHED: 03-18-2013
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Abstract Aim: To investigate the perinatal complications and risk of thyroid dysfunction at early childhood of Graves disease (GD) mothers with euthyroidism (EU) or subclinical hyperthyroidism (sHT) during pregnancy. Method: One hundred and twenty-three pregnant women with GD were recruited. They were all in euthyroidism with treatment of anti-thyroid drugs (ATDs) before pregnancy. All the pregnant GD women maintained EU (n=55) or sHT (n=68) by using ATDs. Sixty randomly selected, age-matched healthy pregnant women (non-GD control) were included. The prenatal and newborn data were collected and analyzed. Toddlers of GD mothers (n=45) and non-GD healthy mothers (n=36) were also recruited for thyroid function and growth assessments. Results: Newborns of mothers with GD had significantly higher complications than those of non-GD mothers. The percent of perinatal complications were 5.0%, 30.9% and 32.3% in the control, EU (vs. control, p<0.001), and sHT (vs. control, p<0.001) groups, respectively. There were no differences between the women continuing low doses of ATDs at the start of pregnancy and the women who stopped receiving ATDs at the start of pregnancy. Toddlers serum levels of FT3, FT4, anti-thyroglobulin antibody, and anti-thyroid peroxidase antibody were significantly higher than those of non-GD mothers (all p<0.05). Conclusion: Pregnancy with GD significantly increases the perinatal complications even with EU. The continued use of ATDs at the start of pregnancy does not give an increased risk of perinatal complications in GD mothers. Maternal GD may also induce a higher risk of autoimmue thyroid dysfunction among offspring at early childhood.
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Formation of the -N(NO)N(NO)- polymer at high pressure and stabilization at ambient conditions.
Proc. Natl. Acad. Sci. U.S.A.
PUBLISHED: 03-15-2013
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A number of exotic structures have been formed through high-pressure chemistry, but applications have been hindered by difficulties in recovering the high-pressure phase to ambient conditions (i.e., one atmosphere and 300 K). Here we use dispersion-corrected density functional theory [PBE-ulg (Perdew-Burke-Ernzerhof flavor of DFT with the universal low gradient correction for long range London dispersion)] to predict that above 60 gigapascal (GPa) the most stable form of N2O (the laughing gas in its molecular form) is a one-dimensional polymer with an all-nitrogen backbone analogous to cis-polyacetylene in which alternate N are bonded (ionic covalent) to O. The analogous trans-polymer is only 0.03?0.10 eV/molecular unit less stable. Upon relaxation to ambient conditions, both polymers relax below 14 GPa to the same stable nonplanar trans-polymer. The predicted phonon spectrum and dissociation kinetics validates the stability of this trans-poly-NNO at ambient conditions, which has potential applications as a type of conducting nonlinear optical polymer with all-nitrogen chains and as a high-energy oxidizer for rocket propulsion. This work illustrates in silico materials discovery particularly in the realm of extreme conditions (very high pressure or temperature).
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Genetic interaction between pku300 and fbn2b controls endocardial cell proliferation and valve development in zebrafish.
J. Cell. Sci.
PUBLISHED: 02-15-2013
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Abnormal cardiac valve morphogenesis is a common cause of human congenital heart disease. The molecular mechanisms regulating endocardial cell proliferation and differentiation into cardiac valves remain largely unknown, although great progress has been made on the endocardial contribution to the atrioventricular cushion and valve formation. We found that scotch tape(te382) (sco(te382)) encodes a novel transmembrane protein that is crucial for endocardial cell proliferation and heart valve development. The zebrafish sco(te382) mutant showed diminished endocardial cell proliferation, lack of heart valve leaflets and abnormal common cardinal and caudal veins. Positional cloning revealed a C946T nonsense mutation of a novel gene pku300 in the sco(te382) locus, which encoded a 540-amino-acid protein on cell membranes with one putative transmembrane domain and three IgG domains. A known G3935T missense mutation of fbn2b was also found ?570 kb away from pku300 in sco(te382) mutants. The genetic mutant sco(pku300), derived from sco(te382), only had the C946T mutation of pku300 and showed reduced numbers of atrial endocardial cells and an abnormal common cardinal vein. Morpholino knockdown of fbn2b led to fewer atrial endocardial cells and an abnormal caudal vein. Knockdown of both pku300 and fbn2b phenocopied these phenotypes in sco(te382) genetic mutants. pku300 transgenic expression in endocardial and endothelial cells, but not myocardial cells, partially rescued the atrial endocardial defects in sco(te382) mutants. Mechanistically, pku300 and fbn2b were required for endocardial cell proliferation, endocardial Notch signaling and the proper formation of endocardial cell adhesion and tight junctions, all of which are crucial for cardiac valve development. We conclude that pku300 and fbn2b represent the few genes capable of regulating endocardial cell proliferation and signaling in zebrafish cardiac valve development.
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A bioactivity-guided study on the anti-diarrheal activity of Polygonum chinense Linn.
J Ethnopharmacol
PUBLISHED: 02-01-2013
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Polygonum chinense Linn., a folk medicine, has long been used for the treatment of diarrhea and enteritis in southwestern China. However, the components responsible for its anti-diarrheal activity are still poorly understood.
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Dose-dependent effects of nicotine on proliferation and differentiation of human bone marrow stromal cells and the antagonistic action of vitamin C.
J. Cell. Biochem.
PUBLISHED: 01-24-2013
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A range of biological and molecular effects caused by nicotine are considered to effect bone metabolism. Vitamin C functions as a biological antioxidant. This study was to evaluate the in vitro effects of nicotine on human bone marrow stromal cells and whether Vitamin C supplementation show the antagonism action to high concentration nicotine. We used CCK-8, alkaline phosphatase (ALP) activity assay, Von Kossa staining, real-time polymerase chain reaction and Western Blot to evaluate the proliferation and osteogenic differentiation. The results indicated that the proliferation of BMSCs increased at the concentration of 50, 100 ng/ml, got inhibited at 1,000 ng/ml. When Vitamin C was added, the OD for proliferation increased. For ALP staining, we found that BMSCs treated with 50 and 100 ng/ml nicotine showed a higher activity compared with the control, and decreased at the 1,000 ng/ml. Bone morphogenetic protein-2 (BMP-2) expression and the calcium depositions decreased at 100 and 1,000 ng/ml nicotine, while the addition of Vitamin C reversed the down regulation. By real-time PCR, we detected that the mRNA expression of collagen type I (COL-I) and ALP were also increased in 50 and 100 ng/ml nicotine groups (P < 0.05), while reduced at 1,000 ng/ml (P < 0.05). When it came to osteocalcin (OCN), the changes were similar. Taken all together, it is found that nicotine has a two-phase effect on human BMSCs, showing that low level of nicotine could promote the proliferation and osteogenic differentiation while the high level display the opposite effect. Vitamin C could antagonize the inhibitory effect of higher concentration of nicotine partly.
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Isolation and Functional Analysis of ZmLTP3, a Homologue to Arabidopsis LTP3.
Int J Mol Sci
PUBLISHED: 01-23-2013
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Plant lipid transfer proteins (LTPs) are encoded by multigene families and play important roles in plant physiology. One full-length cDNA encoding an Arabidopsis LTP3 homologue was isolated from maize by RT-PCR and named as ZmLTP3. RT-PCR analysis indicated that the ZmLTP3 expression is induced by salicylic acid (SA), mannitol and salt. Furthermore, in different tissues the ZmLTP3 displayed different expression patterns, indicating that ZmLTP3 may play multiple roles in stress resistance. Over-expression of ZmLTP3 in wild-type Arabidopsis resulted in the increased salt tolerance. Under salt stress condition, compared to wild-type (WT) plants, transgenic Arabidopsis grew better, had higher seedling fresh (FW), dry weight (DW), seed yields, proline content and lower MDA content and relative electric conductivity level. Our results suggest that maize ZmLTP3 might encode a member of LTPs family and play roles in salt resistance.
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The preferential binding of a sensory organ specific odorant binding protein of the alfalfa plant bug Adelphocoris lineolatus AlinOBP10 to biologically active host plant volatiles.
J. Chem. Ecol.
PUBLISHED: 01-15-2013
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Semiochemicals such as sex pheromones and plant volatiles are crucial components of insect mating systems and host plant localization. In the olfactory signal transduction pathway, odorant-binding proteins (OBPs) are important elements that function in the first step of the pathway by carrying hydrophobic semiochemicals across the sensillum lymph to the olfactory receptors (ORs). In this study, we examined the binding affinities of semiochemicals to AlinOBP10, a putative OBP from the alfalfa plant bug, Adelphocoris lineolatus, that we demonstrate is expressed mainly in sensory organs. We then characterized the biological activities of the high affinity semiochemicals by measuring their electrophysiological activities in antennae and behavioral responses in the plant bug. AlinOBP10 displayed weak binding affinities to two major putative pheromone components, hexyl butyrate and (E)-2-hexenyl butyrate. In contrast, AlinOBP10 exhibited higher binding affinities to six host plant volatiles, namely myrcene, ?-pinene, ?-ionone, 3-hexanone, (E)-2-hexenal, and 1-hexanol. The biological activities of these six putative ligands were further studied in electroantennogram recordings and Y-tube olfactometer trials. The three compounds, (E)-2-hexenal, 1-hexanol, and 3-hexanone elicited strong electrophysiological responses, but elicited distinct behaviors. While 3-hexanone was attractive to female adults, (E)-2-hexenal and 1-hexanol were significant repellents. Although a weak electrophysiological response was elicited with ?-pinene, it was a strong repellent. These results demonstrate that AlinOBP10 can interact with attractants, as well as repellents, with some specificity toward plant volatiles over sex pheromones.
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Arecoline suppresses HaCaT cell proliferation through cell cycle regulatory molecules.
Oncol. Rep.
PUBLISHED: 01-14-2013
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Betel nut chewing is the most common cause of oral submucous fibrosis (OSF). Arecoline is the main component of the betel nut, and is associated with the occurrence and development of OSF through cytotoxicity, genotoxicity and DNA damage. Similar types of stimuli elicit differential responses in different cells. In the present study, we investigated the effects of arecoline on the HaCaT epithelial and Hel fibroblast cell lines. The data showed that arecoline affected HaCaT cell morphology. MTT assay revealed that arecoline suppressed HaCaT cell proliferation. Furthermore, we found that arecoline induced the cell cycle arrest of HaCaT cells. In comparison with the untreated control cells, following treatment with ?75 µg/ml arecoline an increased percentage of HaCaT cells remained at the G0/G1 phase of the cell cycle, accompanied by a reduced percentage of cells in the S phase. However, arecoline treatment did not significantly alter Hel cell cycle distribution. In the HaCaT epithelial cells, arecoline downregulated expression of the G1/S phase regulatory proteins cyclin D1, CDK4, CDK2, E2F1 as determined by reverse transcription-PCR analysis and western blotting. In summary, arecoline inhibits HaCaT epithelial cell proliferation and survival, in a dose-dependent manner, and cell cycle arrest in the G1/S phase, while this is not obvious in the Hel fibroblast cells. Potentially, our findings may aid in the prevention of arecoline-associated human OSF.
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Androgen receptors expressed by prostatic stromal cells obtained from younger versus older males exhibit opposite roles in prostate cancer progression.
Asian J. Androl.
PUBLISHED: 01-09-2013
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Aging is a major risk factor for prostate cancer (PCa), and prostatic stromal cells may also promote PCa progression. Accordingly, stromal cells do not equally promote PCa in older males and younger males. Therefore, it is also possible that the expression of androgen receptors (ARs) by prostatic stromal cells in older versus younger males plays different roles in PCa progression. Using a gene knockdown technique and coculture system, we found that the knockdown of the AR in prostatic stromal cells obtained from younger males could promote the invasiveness and metastasis of cocultured PC3/LNCaP cells in vitro. By contrast, the invasiveness and metastasis of LNCaP cells was inhibited when cocultured with prostatic stromal cells from older males that when AR expression was knocked down. Moreover, after targeting AR expression with small hairpin RNA (shRNA), matrix metalloproteinase (MMP) expression in stromal cells was observed to increase in the younger group, but decreased or remained unchanged in the older group. One exception, however, was observed with MMP9. In vivo, after knocking down AR expression in prostatic stromal cells, the incidence of metastatic lymph nodes was observed to increase in the younger age group, but decreased in the older age group. Together, these data suggest that the AR in prostatic stromal cells played opposite roles in PCa metastasis for older versus younger males. Therefore, collectively, the function of the AR in prostatic stromal cells appears to change with age, and this may account for the increased incidence of PCa in older males.
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Screening for AIP gene mutations in a Han Chinese pituitary adenoma cohort followed by LOH analysis.
Eur. J. Endocrinol.
PUBLISHED: 01-01-2013
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The aryl hydrocarbon receptor interacting protein gene (AIP) is associated with pituitary adenoma (PA). AIP has not been sequenced in East Asian PA populations, so we performed this study in a Han Chinese cohort.
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[Alcohol-induced proliferation of neurons in mouse hippocampal dentate gyrus: a possible role of ceramide].
Sheng Li Xue Bao
PUBLISHED: 12-24-2011
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To investigate the role and mechanism of ceramide (Cer) regulation in alcohol-induced neuronal proliferation and the newborn neurons formation, we used sphingomyelin synthase 2 (predominant enzyme of Cer metabolism) knockout (SMS2(-/-)) and wild type (WT) female mice to establish the model of prenatal alcohol exposure. In 24 h after being given birth (postnatal day 0, P0), the offspring of model mice received blood sphingomyelin (SM) measurement with enzymatic method. On P0, P7, P14 and P30, the proliferation of granule cells in the dentate gyrus and newborn neurons were investigated with immunofluorescent labeling. The expression of protein kinase C? (PKC?) in the hippocampus was tested with Western blot analysis. The results showed that the SM level of blood in SMS2(-/-) pups was significantly lower than that in WT pups. No matter in SMS2(-/-) or WT mice, the prenatal alcohol exposure down-regulated the SM levels in pups with dose-dependency. In both SMS2(-/-) and WT pups, the number of proliferative neurons and newborn neurons in the dentate gyrus gradually decreased with the growing age. Compared with the WT pups, SMS2(-/-) pups showed significantly more proliferative neurons and newborn neurons in the dentate gyrus. Notably, prenatal alcohol exposure dose-dependently increased proliferative neurons and newborn neurons in the dentate gyrus in both WT and SMS2(-/-) pups. The hippocampal expression of PKC? protein in SMS2(-/-) mice was lower than that in WT mice, and prenatal alcohol exposure could up-regulate the PKC? protein expression in both WT and SMS2(-/-) mice with dose dependency. These results suggest that alcohol exposure during pregnancy can induce the compensatory neural cell proliferation and the production of newborn neurons in offspring, and the Cer-ceramide-1-phosphate (C1P) pathway is involved in alcohol-induced neural cell proliferation. The activation of PKC? may be a key step to start the Cer-C1P pathway and up-regulate the alcohol-induced neural cell proliferation and the newborn neurons formation.
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[Analysis of an hereditary coagulation factor XII deficiency in a consanguineous pedigree].
Zhonghua Yi Xue Yi Chuan Xue Za Zhi
PUBLISHED: 12-14-2011
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To analyze genetic mutation and explore its molecular pathogenesis for an hereditary coagulation factor XII(F XII) deficiency in a pedigree featuring consanguineous marriage.
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Analysis of the prognostic factors of very severe aplastic anemia treated with Chinese Kidney-invigorating drugs in combination with anti-lymphocyte globulin or anti-thymocyte globulin.
Chin J Integr Med
PUBLISHED: 12-08-2011
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To explore the prognostic factors for very severe aplastic anemia (VSAA) patients treated mainly with Chinese Kidney (Shen)-invigorating drugs (CKID) combined with anti-lymphocyte globulin (ALG) or anti-thymocyte globulin (ATG).
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[A new retrievable nitinol alloy stents for treatment of refractory benign esophageal strictures].
Zhonghua Wei Chang Wai Ke Za Zhi
PUBLISHED: 11-26-2011
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To explore long-term effect of the treatment of refractory benign esophageal strictures with a novel retrievable fully covered stent made of nitinol alloy.
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Ultrasensitive thermal sensors based on whispering gallery modes in a polymer core optical ring resonator.
Appl Opt
PUBLISHED: 11-24-2011
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This study proposes a thermal sensor based on whispering gallery modes (WGMs) in a polymer core optical ring resonator (PCORR). The thermal sensitivity and detection limit (i.e., the temperature resolution) for WGMs of various orders and polarizations are theoretically studied as a function of the ring wall thickness. The results show that the temperature detection limits can be as low as 4×10(-5) and 6×10(-6) ?K for laser linewidths of 2 and 0.3 MHz, respectively. The ultrahigh temperature resolution makes the PCORR a very promising platform for temperature measurement. The analysis also shows that the WGM of a lower order has better thermal sensing performance and a thinner optimal thickness of the ring resonator.
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[Effect of neotype carbonic anhydrase target-based inhibitors(P-8) on the hypoxic tolerance in mice].
Zhongguo Ying Yong Sheng Li Xue Za Zhi
PUBLISHED: 11-22-2011
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To explore the effects of different doses of P-8 in increasing the Hypoxia tolerance of mice and the mechanisms involved.
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[Altered expression of endogenous transforming growth factor ?1 and early calcification related genes in rat endplate].
Zhonghua Yi Xue Za Zhi
PUBLISHED: 11-19-2011
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To explore the relationship between endogenous transforming growth factor (TGF)-?1 and calcification-related genes through an in vitro degeneration model by propagating rat endplate chondrocytes during a natural degeneration process.
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Down-regulation of peroxisome proliferator-activated receptor ? coactivator-1? expression in fatty acid-induced pancreatic beta-cell apoptosis involves nuclear factor-?B pathway.
Chin. Med. J.
PUBLISHED: 11-16-2011
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Pancreatic beta-cell apoptosis induced by lipotoxicity, to a large extent, contributes to the progression of type 2 diabetes. To investigate the mechanism of free fatty acid induced apoptosis, we aimed to study the effects of palmitic acid (PA) on the apoptosis and peroxisome proliferator-activated receptor ? coactivator-1? (PGC-1?) expression in ?TC3 cells as well as the possible role of nuclear factor-?B (NF-?B) in this process.
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Altered nuclear factor-kappaB inducing kinase expression in insulin-resistant mice.
Chin. Med. J.
PUBLISHED: 11-16-2011
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Insulin resistance is an underlying feature of both type 2 diabetes and metabolic syndrome. Currently, it is unclear whether nuclear factor (NF)-?B inducing kinase (NIK) plays a role in the development of insulin resistance. The present in vivo study investigated the roles of NIK and I?B kinase ? (IKK?) in obesity-induced insulin resistance using animal models.
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The effect of repeated etomidate anesthesia on adrenocortical function during a course of electroconvulsive therapy.
J ECT
PUBLISHED: 11-15-2011
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Etomidate may affect adrenocortical function. We conducted an investigation of the comparative effects of etomidate and propofol during electroconvulsive therapy (ECT) on adrenocortical function and hemodynamics.
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What is Visualize?

JoVE Visualize is a tool created to match the last 5 years of PubMed publications to methods in JoVE's video library.

How does it work?

We use abstracts found on PubMed and match them to JoVE videos to create a list of 10 to 30 related methods videos.

Video X seems to be unrelated to Abstract Y...

In developing our video relationships, we compare around 5 million PubMed articles to our library of over 4,500 methods videos. In some cases the language used in the PubMed abstracts makes matching that content to a JoVE video difficult. In other cases, there happens not to be any content in our video library that is relevant to the topic of a given abstract. In these cases, our algorithms are trying their best to display videos with relevant content, which can sometimes result in matched videos with only a slight relation.