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Find video protocols related to scientific articles indexed in Pubmed.
Iterative algorithm for absolute planarity calibration in three-flat test.
Opt Express
PUBLISHED: 11-18-2014
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An iterative algorithm is presented to calibrate the shapes of the reference surfaces in three-flat test. Three measurements in three-flat test determine the even part of the flat surfaces. The odd part can be obtained by adding one or more rotation measurements and using analytical recovery algorithm. Here we present an iterative algorithm to obtain the odd part instead of special analytical recovery algorithm. The iterative algorithm is purely numerical, and only involved in data rotation operation. A simulation experiment proves the validity and high accuracy of the proposed algorithm method.
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The complete mitochondrial genome of Schizopygopsis chengi baoxingensis (Teleostei, Cyprinidae, Schizopygopsis).
Mitochondrial DNA
PUBLISHED: 11-13-2014
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Abstract Schizopygopsis chengi baoxingensis is endemic to the Baoxing River in China and may become an endangered species due to its very narrow habitat and the construction of hydropower dams. In this study, we successfully sequenced the first mitochondrial genome of S. c. baoxingensis. The mitogenome is 16,787?bp in length, containing 13 protein-coding genes, two rRNA genes, 22 tRNA genes and a control region (D-loop). The overall base composition of the H-strand is 28.5% A, 26.2% T, 18.4% C, and 26.9% G, with a slight AT bias of 54.7%. The sequenced mtDNA genome of S. c. baoxingensis is similar in gene arrangement to that of other cyprinidaes except for a 191?bp non-coding region found between tRNA(Thr) and tRNA(Pro).
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Clinical utility of simultaneous measurement of alpha-fetoprotein and des-?-carboxy prothrombin for diagnosis of patients with hepatocellular carcinoma in China: A multi-center case-controlled study of 1,153 subjects.
Biosci Trends
PUBLISHED: 11-11-2014
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This study aimed to investigate the clinical utility of simultaneous measurement of alphafetoprotein (AFP) and des-?-carboxy prothrombin (DCP) for hepatocellular carcinoma (HCC) diagnosis in Chinese patients predominantly caused by hepatitis B virus infection by a multi-center case-controlled study. Subjects were 1,153 individuals from three major hospitals in China, including 550 cases in HCC group, 164 in Malignant disease group, 182 in Benign disease group, 85 in Chronic liver disease group, and 173 in Normal group. Serum levels of AFP and DCP were measured and clinicopathological features were determined for all subjects. Results showed that the levels of DCP and AFP were significantly higher in HCC group (550 patients, 74.18% with HBV infection) than that in other four groups (P < 0.001). Receiver operating curves (ROC) indicated the optimal cut-off value was 86 mAU/mL for DCP with a sensitivity of 71.50% and specificity of 86.30%, and 21 ng/mL for AFP with a sensitivity of 68.00% and specificity of 93.20%. The area under ROC curve was 0.846 for DCP, 0.832 for AFP, and 0.890 for the combination of DCP and AFP. The combination of DCP and AFP resulted in a higher Youden index and a sensitivity of approximately 90%, even for small tumors. The simultaneous measurement of AFP and DCP could achieve a better sensitivity in diagnosing Chinese HCC patients, even for small tumors.
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Respiratory motion management using audio-visual biofeedback for respiratory-gated radiotherapy of synchrotron-based pulsed heavy-ion beam delivery.
Med Phys
PUBLISHED: 11-06-2014
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To efficiently deliver respiratory-gated radiation during synchrotron-based pulsed heavy-ion radiotherapy, a novel respiratory guidance method combining a personalized audio-visual biofeedback (BFB) system, breath hold (BH), and synchrotron-based gating was designed to help patients synchronize their respiratory patterns with synchrotron pulses and to overcome typical limitations such as low efficiency, residual motion, and discomfort.
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Flavobacterium suzhouense sp. nov., isolated from farmland river sludge.
Int. J. Syst. Evol. Microbiol.
PUBLISHED: 11-02-2014
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A Gram-negative bacterium, designated XIN-1T, was isolated from farmland river sludge sample in Suzhou, China. Cells of strain XIN-1T was strictly aerobic, non-motile and rod-shaped. Strain XIN-1T grew optimally at pH 7.0 and 28?. Phylogenetic analysis of the 16S rRNA gene sequences showed that strain XIN-1T was most closely related to Flavobacterium hauense BX12T (98.2 %), followed by Flavobacterium beibuense F44-8T (96.3 %). The major respiratory quinone was menaquinone-6 and the major polar lipid was phosphatidylethanolamine. The major fatty acids (?5 %) were summed feature 3 (comprising C16:1?7c and/or C16:1?6c), summed feature 4 (comprising C17:1iso I and/or C17:1anteiso B), iso-C15:0, C16:0 and iso-C17:0 3-OH. The genomic DNA G+C content of strain XIN-1T was 39.8 mol %. Strain XIN-1T showed low DNA-DNA relatedness with F.hauense BX12T (38.7±0.5 %). On the basis of genotypic and phenotypic data, strain XIN-1T is considered to represent a novel species of the genus Flavobacterium, for which the name Flavobacterium suzhouense sp. nov. is proposed. The type strain is XIN-1T (=CCTCC AB 2014200T =KCTC 42107T).
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Differential activation of receptors and signal pathways upon stimulation by different doses of sphingosine-1-phosphate in endothelial cells.
Exp. Physiol.
PUBLISHED: 11-02-2014
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Sphingosine-1-phosphate (S1P) is a bioactive sphingolipid in plasma, and its plasma concentration can be adjusted through a complex metabolic process. The alterations in S1P levels and the activation of receptors collaboratively regulate distinct biological effects. This study was performed to comparatively investigate the effect of different concentrations of S1P on endothelial barrier function and to explore the roles of S1P receptors (S1PRs), Rho GTPases and calcium in S1P-induced endothelial responses. Endothelial barrier function was studied using transendothelial electric resistance (TEER) and a resistance meter in human umbilical vein endothelial cells (HUVECs). Specific agonists or antagonists were applied to control the activation of S1P receptors and the release of calcium from different cellular compartments. The results indicated that at physiological concentrations, S1P preserved endothelial barrier function by binding with S1PR1. The activation of S1PR1 triggered the release of intracellular Ca(2+) from the ER through the PI-PLC and IP3 pathways. Consequently, the Rho GTPase Rac1 was activated, strengthening the assembly of tight junction proteins and F-actin. However, excessive S1P induced endothelial barrier dysfunction by activating S1PR2 followed by the RhoA/RhoA kinase pathway, causing the disorganisation of F-actin and the disassembly of the tight junction protein ZO-1. An influx of extracellular Ca(2+) was involved in this process. These data suggested that physiological and excessive amounts of S1P induce different responses in HUVECs; the activation of the 1PR1-PLC-IP3R-Ca(2+)-Rac1 pathway governs the low-dose S1P-enhanced endothelial barrier integrity, and the activation of S1PR2-calcium influx-RhoA/ROCK dominates the high-dose S1P-induced endothelial monolayer hyperpermeability response. This article is protected by copyright. All rights reserved.
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Design and Optimization of Novel Hydroxamate-Based Histone Deacetylase Inhibitors of Bis-Substituted Aromatic Amides Bearing Potent Activities against Tumor Growth and Metastasis.
J. Med. Chem.
PUBLISHED: 11-01-2014
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Histone deacetylases (HDACs) are one of the most promising drug targets for cancer therapy, and since more than 90% of all cancer-related deaths are associated with tumor metastasis, developing strategies to inhibit tumor metastasis while retaining anti-tumor growth activity are of great interest. Herein we demonstrated the design and identification of a series of novel hydroxamate-based HDAC inhibitors bearing potent activities against tumor growth and metastasis. Optimization of the initial hit resulted in the discovery of new HDAC inhibitors through studying the structure-activity relationship. Among them, compound 11b, one of the most potent leads, exhibited nanomolar IC50 values toward inhibition of class I and IIb HDACs as well as sub-micromolar activity against proliferation and migration of breast cancer cells in vitro. More importantly, it also significantly suppressed tumor growth in a breast tumor xenograft mouse model and dose-dependently blocked in vivo tumor metastasis in a mouse pulmonary metastasis model.
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Changes in tar yields and cigarette design in samples of Chinese cigarettes, 2009 and 2012.
Tob Control
PUBLISHED: 10-30-2014
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China is home to the greatest number of smokers as well as the greatest number of smoking-related deaths. An active and growing market of cigarettes marketed as 'light' or 'low tar' may keep health-concerned smokers from quitting, wrongly believing that such brands are less harmful.
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[Lentivirus-mediated stable Fas gene silencing in human umbilical cord-derived mesenchymal stem cells].
Nan Fang Yi Ke Da Xue Xue Bao
PUBLISHED: 10-28-2014
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To construct a lentivirus-mediated vector for RNA interference (RNAi) of Fas and establish a human umbilical cord-derived mesenchymal stem cells (UC-MSCs) line with stable Fas gene silencing.
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Repair of Extrahepatic Bile Duct Defect Using a Collagen Patch in a Swine Model.
Artif Organs
PUBLISHED: 10-28-2014
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Extrahepatic bile duct (EBD) injury can happen during surgery. To repair a defect of the EBD and prevent postoperative biliary complications, a collagen membrane was designed. The collagen material was porous, biocompatible, and degradable and could maintain its shape in bile soaking for about 4 weeks. The goal was to induce rapid bile duct tissue regeneration. Twenty Chinese experimental hybrid pigs were used in this study and divided into a patch group and a control group. A spindle-shaped defect (20?mm?×?6?mm) was made in the anterior wall of the lower EBD in the swine model, and then the defect was reconstructed using a collagen patch with a drainage tube and wrapped with greater omentum. Ultrasound was performed at 2, 4, 8, and 12 weeks postoperatively. Liver function tests and white blood cell count (WBC) were measured. Hematoxylin-eosin staining, cytokeratin 7 immunohistochemical staining, and Van Gieson's staining of EBD were used. The diameter and thickness of the EBD at the graft site were measured. There was no significant difference in liver function tests or WBC in the patch group compared with the control group. No evidence of leakage or stricture was observed, but some pigs developed biliary sludge or stone at 4 and 8 weeks. The drainage tube was lost within 12 weeks. The neo-EBD could withstand normal biliary pressure 2 weeks after surgery. Histological study showed the accessory glands and epithelial cells gradually regenerated at graft sites from 4 weeks, with increasing vessel infiltration and decreasing inflammation. The collagen fibers became regular with full coverage of epithelial cells. The statistical analysis of diameter and thickness showed no stricture formation at the graft site, but the EBD wall was slightly thicker than in the normal bile duct due to collagen fiber deposition. The structure of the neo-EBD was similar to that of the normal EBD. The collagen membrane patch associated with a drainage tube and wrapped with greater omentum effectively induced the regeneration of the EBD defect within 12 weeks.
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Toxic metal and nicotine content of cigarettes sold in China, 2009 and 2012.
Tob Control
PUBLISHED: 10-23-2014
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Metals of primary health concern can accumulate in the tobacco plant and contribute to smokers' exposures to carcinogens, a significant cause of the millions of smoking-related deaths in China each year. These exposures are due to the smoker's addiction to nicotine.
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A Meta-Analysis of the Correlation Between the HLA-DRB1*03 Allele and Chronic Hepatitis B in the Han Chinese Population.
Genet Test Mol Biomarkers
PUBLISHED: 10-18-2014
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Objective: This study sought to use a meta-analysis approach to comprehensively evaluate correlations between the human leukocyte antigen-DR beta 1 (HLA-DRB1)*03 allele and chronic hepatitis B (CHB) in the Han Chinese population. Methods: The China Biomedical Literature database (CBMdisc), the Chongqing VIP database (VIP), and the PubMed database were searched. Using the inclusion and exclusion criteria of this study, all relevant case-control studies retrieved in these searches that satisfied the conditions of this investigation were collected. Review Manager (RevMan) 5.2 software was used to conduct a meta-analysis on the results of these studies. Results: There were 9 publications that satisfied the inclusion criteria. These publications included a total of 970 cases in the CHB group and 1185 cases in the normal control group. Egger's test revealed no significant publication bias. A comprehensive analysis indicated that the pooled odds ratio (OR) value was 1.94 with a 95% confidence interval (CI) of 1.23-3.06 (Z=2.84, p=0.004); these findings suggested that in the Han Chinese population, the HLA-DRB1*03 allele is a susceptibility allele related to the occurrence of CHB. Conclusion: There is a statistically significant correlation between the HLA-DRB1*03 allele and the occurrence of CHB in the Han Chinese population, and the HLA-DRB1*03 allele may be a susceptibility allele for this disease.
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Endovascular Treatment for Fusiform Dilation of Internal Carotid Artery Following Craniopharyngioma Resection: A Case Illustration.
J. Child Neurol.
PUBLISHED: 10-16-2014
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Fusiform dilation of the internal carotid artery complicates aggressive craniopharyngioma resection and occurs mainly in children. We report a case to describe the availability of endovascular treatment for this rare entity. A 13-year-old boy presented with headache for 2 years after resection of craniopharyngioma. A fusiform dilation of the right carotid artery was found and was coiled using stent-assisted technique. Follow-up showed satisfactory outcome and disappearance of headache. To our knowledge, this is the first report regarding endovascular treatment for fusiform dilation of the internal carotid artery after craniopharyngioma resection. Stent-assisted coiling is a useful approach for fusiform dilation of the internal carotid artery following craniopharyngioma surgery.
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WNT5A modulates cell cycle progression and contributes to the chemoresistance in pancreatic cancer cells.
HBPD INT
PUBLISHED: 10-14-2014
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Although there are many studies on the mechanism of chemoresistance in cancers, studies on the relations between WNT5A and chemoresistance in pancreatic cancer are rare. The present study was to examine the role of WNT5A in the regulation of cell cycle progression and in chemo-resistance in pancreatic cancer tissues and cell lines.
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[Analysis of clinical phenotype and genetic mutations of a pedigree of familial hemophagocytic lymphohistiocytosis].
Zhonghua Yi Xue Yi Chuan Xue Za Zhi
PUBLISHED: 10-10-2014
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To analyze mutations in a pedigree of familial hemophagocytic lymphohistiocytosis (FHLH) from Sichuan and provide genetic counseling for the family.
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Presynaptic BK Channels Modulate Ethanol-Induced Enhancement of GABAergic Transmission in the Rat Central Amygdala Nucleus.
J. Neurosci.
PUBLISHED: 10-10-2014
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Large-conductance calcium-activated potassium BK channels are widely expressed in the brain and are involved in the regulation of neuronal functions such as neurotransmitter release. However, their possible role in mediating ethanol-induced GABA release is still unknown. We assessed the role of BK channels in modulating the action of ethanol on inhibitory synaptic transmission mediated via GABAA receptors in the rat central nucleus of the amygdala (CeA). Evoked IPSCs (eIPSCs) mediated by GABAA receptors were isolated from CeA neurons under whole-cell voltage clamp, and their response to selective BK channel antagonists, channel activators, or ethanol was analyzed. Blocking BK channels with the specific BK channel antagonist paxilline significantly increased the mean amplitude of eIPSCs, whereas the activation of BK channels with the channel opener NS1619 reversibly attenuated the mean amplitude of eIPSCs. Ethanol (50 mm) alone enhanced the amplitude of eIPSCs but failed to further enhance eIPSCs in the slices pretreated with paxilline. Bath application of either BK channel blockers significantly increased the frequency of miniature IPSCs (mIPSCs). Similarly, 50 mm ethanol alone also enhanced mIPSC frequency. Increases in mIPSC frequency by either selective BK channel antagonists or ethanol were not accompanied with changes in the amplitude of mIPSCs. Furthermore, following bath application of BK channel blockers for 10 min, ethanol failed to further increase mIPSC frequency. Together, these results suggest that blocking BK channels mimics the effects of ethanol on GABA release and that presynaptic BK channels could serve as a target for ethanol effects in CeA.
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Preparation of 5-fluorouracil-loaded Nanoparticles and Study of Interaction with Gastric Cancer Cells.
Asian Pac. J. Cancer Prev.
PUBLISHED: 10-09-2014
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To prepare 5-fluorouracil (5-Fu) nanoparticles with higher encapsulation efficiency and drug loading, and then investigate interaction with the SGC-7901 gastric cancer cell line.
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Visceral adiposity index and insulin secretion and action in first-degree relatives of subjects with type 2 diabetes.
Diabetes Metab. Res. Rev.
PUBLISHED: 10-06-2014
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To explore the predictive performance of the newly established visceral adiposity index (VAI) for diabetes and prediabetes, as well as the relationships between the VAI and the parameters of insulin secretion and action.
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[Effects of plant-derived smoke water on accumulation of biomass and active substance of Salvia miltiorrhiza f. alba].
Zhongguo Zhong Yao Za Zhi
PUBLISHED: 10-04-2014
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To study the effect of plant-derived smoke water on the accumulation of biomass and active substance of Salvia miltiorrhiza f. alba, seedlings of S. miltiorrhiza were treated with different concentrations of smoke water (1:500, 1: 1 000, 1: 2 000). The fresh weight and dry weight of underground part, the number of split-root, maximum root diameter, average root diameter, average root length, the content of lipophilic components and water-soluble components were measured. Results showed that fresh weight and dry weight of underground part were respectively improved by 98.01%, 44.32% and 85.71%, 28.57% with significant difference by smoke water treatment with concentration of 1: 500 and 1: 1 000. Maximum root diameter and dry weight of underground part were respectively enhanced by 58.44% and 85.71% by smoke water with concentration of 1:500. The content of tanshinone I and tanshinone II(A) were improved by smoke water treatment, however there were no significantly difference on the content of cryptotanshinone and dihydrotan shinone. This study indicates that smoke water treatment could be used to improve the accumulation of biomass and active substance content of S. miltiorrhiza f. alba, which could provide new ideas for its green cultivating.
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Surgical treatment of traumatic multiple intracranial hematomas.
Neurosciences (Riyadh)
PUBLISHED: 10-03-2014
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To summarize our experience with the surgical treatment of traumatic multiple intracranial hematomas (TMIHs) and discuss the surgical indications.
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[Comparison of thoracoscopic esophagectomy and traditional esophagectomy in radical mediastinal lymphadenectomy for esophageal cancer].
Zhonghua Wei Chang Wai Ke Za Zhi
PUBLISHED: 10-03-2014
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To compare thoracoscopic esophagectomy with traditional esophagectomy in radical mediastinal lymphadenectomy for esophageal cancer, and to explore the feasibility and safety of thoracoscopic mediastinal lymphadenectomy for esophagectomy.
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A novel mutation in the CD40 ligand gene in a Chinese boy with X-linked hyper-IgM syndrome.
Asian Pac. J. Allergy Immunol.
PUBLISHED: 10-01-2014
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X-linked hyper-IgM Syndrome (XHIGM) is caused by a mutation of CD40 ligand (CD40L), which is normally expressed on activated CD4+ T cells and is responsible for immunoglobulin class switching. A 7-year-old boy with recurrent sino-pulmonary infections since the age of 3 months had normal CD3+, CD4+, CD8+T lymphocytes, and CD19+B lymphocytes and NK cells, but significantly elevated IgM and extremely decreased IgG and IgA. Sequencing of genomic DNA revealed that the patient had a 34 base deletion in intron 3 and exon 4 of CD40L(g.8172_8205del34bp), which lead to the entire deletion of exon 4 in cDNA (c.347_409del63bp, i.e.,exon 4 skipping) and an in-frame deletion of 21 amino acids in CD40L protein. Moreover, the patient had negligible CD40L expression on activated CD3+CD8-T lymphocytes. His mother and sister were carriers of the CD40L mutation. Our studies demonstrated a novel mutation in CD40L, which, to our knowledge, has not been reported previously.
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Activation of TRPV1 Attenuates High Salt Induced Cardiac Hypertrophy through Improvement of Mitochondrial Function.
Br. J. Pharmacol.
PUBLISHED: 09-28-2014
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High salt diet induces cardiac remodelling and leads to heart failure, which is closely related to cardiac mitochondrial dysfunction. Transient receptor potential channels are implicated in the pathogenesis of cardiac dysfunction. We investigated whether activation of transient receptor potential vanilloid (subtype 1) (TRPV1) by dietary capsaicin can prevent high salt diet-induced cardiac hypertrophy through the improvement of cardiac mitochondrial dysfunction.
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[Case-control study on minimally invasive percutaneous plate osteosynthesis for the treatment of distal tibial comminuted fractures at different operation times].
Zhongguo Gu Shang
PUBLISHED: 09-23-2014
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To compare clinical outcomes of minimally invasive percutaneous plate osteosynthesis (MIPPO) in treating distal tibial comminuted fractures at early and delayed stage.
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Gastrointestinal intervention ameliorates high blood pressure through antagonizing overdrive of the sympathetic nerve in hypertensive patients and rats.
J Am Heart Assoc
PUBLISHED: 09-21-2014
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We investigated the hypothesis that the favorable effects of gastrointestinal (GI) intervention on hypertension (HTN) and cardiovascular (CV) disturbances are mediated by antagonizing overdrive of the sympathetic nervous system (SNS).
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Follow-up of blood-pressure lowering and glucose control in type 2 diabetes.
N. Engl. J. Med.
PUBLISHED: 09-19-2014
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In the Action in Diabetes and Vascular Disease: Preterax and Diamicron Modified Release Controlled Evaluation (ADVANCE) factorial trial, the combination of perindopril and indapamide reduced mortality among patients with type 2 diabetes, but intensive glucose control, targeting a glycated hemoglobin level of less than 6.5%, did not. We now report results of the 6-year post-trial follow-up.
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Hyperbaric oxygen preconditioning protects lung against hyperoxic acute lung injury in rats via heme oxygenase-1 induction.
Biochem. Biophys. Res. Commun.
PUBLISHED: 09-15-2014
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Hyperoxic acute lung injury (HALI) is a clinical syndrome as a result of prolonged supplement of high concentrations of oxygen. Previous studies have shown hyperbaric oxygen preconditioning (HBO-PC) had a protective effect on oxidative injury. In the present study, we investigated the effect of HBO-PC on HALI in rats. The results demonstrated that HBO-PC ameliorated the lung biochemical and histological alterations induced by hyperoxia, decreased oxidative products but increased antioxidant enzymes. Furthermore, HBO-PC up-regulated heme oxygenase-1 (HO-1) mRNA and activity in lung tissues. The administration of HO-1 inhibitor, Zinc protoporphyrin IX, abolished its protective effects. The data showed that HBO-PC could protect rats against HALI and the anti-oxidative effect may be related to the up-regulation of HO-1.
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Predicting subsequent relapse by drug-related cue-induced brain activation in heroin addiction: an event-related functional magnetic resonance imaging study.
Addict Biol
PUBLISHED: 09-13-2014
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Abnormal salience attribution is implicated in heroin addiction. Previously, combining functional magnetic resonance imaging (fMRI) and a drug cue-reactivity task, we demonstrated abnormal patterns of subjective response and brain reactivity in heroin-dependent individuals. However, whether the changes in cue-induced brain response were related to relapse was unknown. In a prospective study, we recruited 49 heroin-dependent patients under methadone maintenance treatment, a gold standard treatment (average daily dose 41.8?±?16.0?mg), and 20 healthy subjects to perform the heroin cue-reactivity task during fMRI. The patients' subjective craving was evaluated. They participated in a follow-up assessment for 3 months, during which heroin use was assessed and relapse was confirmed by self-reported relapse or urine toxicology. Differences between relapsers and non-relapsers were analyzed with respect to the results from heroin-cue responses. Compared with healthy subjects, relapsers and non-relapsers commonly demonstrated significantly increased brain responses during the processing of heroin cues in the mesolimbic system, prefrontal regions and visuospatial-attention regions. However, compared with non-relapsers, relapsers demonstrated significantly greater cue-induced craving and the brain response mainly in the bilateral nucleus accumbens/subcallosal cortex and cerebellum. Although the cue-induced heroin craving was low in absolute measures, the change in craving positively correlated with the activation of the nucleus accumbens/subcallosal cortex among the patients. These findings suggest that in treatment-seeking heroin-dependent individuals, greater cue-induced craving and greater specific regional activations might be related to reward/craving and memory retrieval processes. These responses may predict relapse and represent important targets for the development of new treatment for heroin addiction.
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Pharmacokinetic comparisons of benzoylmesaconine in rats using ultra-performance liquid chromatography-tandem mass spectrometry after administration of pure benzoylmesaconine and wutou decoction.
Molecules
PUBLISHED: 09-11-2014
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Wutou decoction is widely used in China because of its therapeutic effect on rheumatoid arthritis. Benzoylmesaconine (BMA), the most abundant component of Wutou decoction, was used as the marker compound for the pharmacokinetic study of Wutou decoction. The aim of the present study was to compare the pharmacokinetics of BMA in rats after oral administration of pure BMA and Wutou decoction. Pure BMA (5 mg/kg) and Wutou decoction (0.54 g/kg, equivalent to 5 mg/kg BMA) were orally administered to rats with blood samples collected over 10 h. Quanti?cation of BMA in rat plasma was achieved using sensitive and validated ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). Specifically, the half-life (T1/2) and mean residence time values of pure BMA were 228.3 ± 117.0 min and 155.0 ± 33.2 min, respectively, whereas those of BMA in Wutou decoction were decreased to 61.8 ± 35.1 min and 55.8 ± 16.4 min, respectively. The area under the curve (AUC) of BMA after administration of Wutou decoction was significantly decreased (five-fold) compared with that of pure BMA. The results indicate that the elimination of BMA in rats after the administration of Wutou decoction was significantly faster compared with that of pure BMA.
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[Determination of tauroursodeoxycholic acid in compound bile capsule by HPLC].
Zhongguo Zhong Yao Za Zhi
PUBLISHED: 09-11-2014
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To discriminate and determine of the artificial bear bile of the compound bile capsule.
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Effect of segmentation algorithms on the performance of computerized detection of lung nodules in CT.
Med Phys
PUBLISHED: 09-05-2014
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The purpose of this study is to reveal how the performance of lung nodule segmentation algorithm impacts the performance of lung nodule detection, and to provide guidelines for choosing an appropriate segmentation algorithm with appropriate parameters in a computer-aided detection (CAD) scheme.
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Construction of a host-independent T7 expression system with small RNA regulation.
J. Biotechnol.
PUBLISHED: 09-02-2014
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It is desirable to build a universal and efficient protein expression system for wild-type prokaryotic strains in biotechnology industry and the outstanding T7 expression system could be a good candidate. However, the current utilization of T7 system depends on the specific DE3 lysogenic hosts, which severely limits its application in wild-type strains. In this study, a host-independent T7 expression system without relying on DE3 lysogenic hosts to provide T7 RNA Polymerase was developed. T7 RNA Polymerase gene (Gene1) and T7 Promoter were successfully integrated into a single plasmid with the regulation of proper antisense RNA to limit T7 RNA Polymerase expression at a non-lethal level. This host-independent T7 expression system realized efficient protein expression in 4 non-DE3 Escherichia coli strains and a wild-type Sinorhizobium strain TH572.
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[A comparative study of three-dimensional versus two-dimensional laparoscopic subtotal thyroidectomy via a breast approach].
Nan Fang Yi Ke Da Xue Xue Bao
PUBLISHED: 09-02-2014
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To explore the safety, efficacy and feasibility of 3D laparoscopic subtotal thyroidectomy via a breast approach.
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Optical fiber-based system for continuous measurement of in-bore projectile velocity.
Rev Sci Instrum
PUBLISHED: 09-01-2014
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This paper reports the design of an optical fiber-based velocity measurement system and its application in measuring the in-bore projectile velocity. The measurement principle of the implemented system is based on Doppler effect and heterodyne detection technique. The analysis of the velocity measurement principle deduces the relationship between the projectile velocity and the instantaneous frequency (IF) of the optical fiber-based system output signal. To extract the IF of the fast-changing signal carrying the velocity information, an IF extraction algorithm based on the continuous wavelet transforms is detailed. Besides, the performance of the algorithm is analyzed by performing corresponding simulation. At last, an in-bore projectile velocity measurement experiment with a sniper rifle having a 720 m/s muzzle velocity is performed to verify the feasibility of the optical fiber-based velocity measurement system. Experiment results show that the measured muzzle velocity is 718.61 m/s, and the relative uncertainty of the measured muzzle velocity is approximately 0.021%.
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Pneumothorax in cystic fibrosis.
J Thorac Dis
PUBLISHED: 08-31-2014
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Pneumothorax is recognized as a common and life-threatening complication in cystic fibrosis (CF) patients, especially in those who are infected with P. aeruginosa, B. cepacia or Aspergillus, need enteral feeding, are diagnosed as suffering from allergic bronchopulmonary aspergillosis (ABPA), developed massive hemoptysis, and their respiratory function is seriously compromised. Structural impairment and altered airflow dynamics in the lungs of CF patients are considered as the main predisposing factors, but also inhaled medications and non-invasive positive pressure ventilation (NIPPV) could increase the risk of pneumothorax. Clinical presentation could range from dramatic to very mild. Management of spontaneous pneumothorax occurring to patients with CF is essentially similar to that for non-CF patients. Therapeutic options include intercostal tube drainage, video-assisted thoracoscopic surgery (VATS), and medical or surgical pleurodesis. Pneumothorax increases both short- and long-term morbidity and mortality in CF patients and causes significant deterioration of their quality of life.
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Genome sequence and genetic diversity of the common carp, Cyprinus carpio.
Nat. Genet.
PUBLISHED: 08-29-2014
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The common carp, Cyprinus carpio, is one of the most important cyprinid species and globally accounts for 10% of freshwater aquaculture production. Here we present a draft genome of domesticated C. carpio (strain Songpu), whose current assembly contains 52,610 protein-coding genes and approximately 92.3% coverage of its paleotetraploidized genome (2n = 100). The latest round of whole-genome duplication has been estimated to have occurred approximately 8.2 million years ago. Genome resequencing of 33 representative individuals from worldwide populations demonstrates a single origin for C. carpio in 2 subspecies (C. carpio Haematopterus and C. carpio carpio). Integrative genomic and transcriptomic analyses were used to identify loci potentially associated with traits including scaling patterns and skin color. In combination with the high-resolution genetic map, the draft genome paves the way for better molecular studies and improved genome-assisted breeding of C. carpio and other closely related species.
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Mechanical properties and structural features of novel Fe-based bulk metallic glasses with unprecedented plasticity.
Sci Rep
PUBLISHED: 08-29-2014
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Fe-based bulk metallic glasses (BMGs) have attracted great attention due to their unique magnetic and mechanical properties, but few applications have been materialized because of their brittleness at room temperature. Here we report a new Fe(50)Ni(30)P(13)C(7) BMG which exhibits unprecedented compressive plasticity (>20%) at room temperature without final fracture. The mechanism of unprecedented plasticity for this new Fe-based BMG was also investigated. It was discovered that the ductile Fe(50)Ni(30)P(13)C(7) BMG is composed of unique clusters mainly linked by less directional metal-metal bonds which are inclined to accommodate shear strain and absorbed energy in the front of crack tip. This conclusion was further verified by the X-ray photoelectron spectroscopy and ultraviolet photoelectron spectroscopy experiments of Fe(80-x)Ni(x)P(13)C(7) (x = 0, 10, 20, 30) and Fe(72-x)Ni(x)B(20)Si(4)Nb(4) (x = 0, 7.2, 14.4, 21.6, 28.8) glassy systems. The results also indicate a strong correlation between the p-d hybridization and plasticity, verifying that the transition from brittle to ductile induced by Ni addition is due to the change of bonding characteristics in atomic configurations. Thus, we can design the plasticity of Fe-based BMGs and open up a new possible pathway for manufacturing BMGs with high strength and plasticity.
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Gold nanoparticle transfer through photothermal effects in a metamaterial absorber by nanosecond laser.
Sci Rep
PUBLISHED: 08-26-2014
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A non-complicated, controllable method of metallic nanoparticle fabrication at low operating light power is proposed. The method is based on laser-induced forward transfer, using a metamaterial absorber as the donor to significantly enhance the photothermal effect and reduce the operating light fluence to 35?mJ/cm(2), which is much lower than that in previous works. A large number of metallic nanoparticles can be transferred by one shot of focused nanosecond laser pulses. Transferred nanoparticles exhibit good size uniformity and the sizes are controllable. The optical properties of transferred particles are characterized by dark-field spectroscopy and the experimental results agree with the simulation results.
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Tube thoracostomy; chest tube implantation and follow up.
J Thorac Dis
PUBLISHED: 08-25-2014
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Pneumothorax is an urgent medical situation that requires urgent treatment. We can divide this entity based on the etiology to primary and secondary. Chest tube implantation can be performed either in the upper chest wall or lower. Both thoracic surgeons and pulmonary physicians can place a chest tube with minimal invasive techniques. In our current work, we will demonstrate chest tube implantation to locations, methodology and tools.
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Pneumothorax in sarcoidosis.
J Thorac Dis
PUBLISHED: 08-25-2014
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While sarcoidosis is a relatively common but often underdiagnosed disease, pneumothorax appears to be a rare clinical event observed mostly at the late stages of the disease course. The precise underlying mechanism of such complication is unclear and probably involves the formation of bullae due to bronchial obstruction and retraction-collapse of distracted lung tissue. Thoracoscopic bullectomy represent the preferred treatment option for recurrent pneumothoraces. The administration of corticosteroids for the treatment of pulmonary sarcoidosis may have a protecting effect for such recurrences, but remains a controversial issue.
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Penetrating trauma.
J Thorac Dis
PUBLISHED: 08-25-2014
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Pneumothorax occurs when air enters the pleural space. Currently there is increasing incidence of road traffic accidents, increasing awareness of healthcare leading to more advanced diagnostic procedures, and increasing number of admissions in intensive care units are responsible for traumatic (non iatrogenic and iatrogenic) pneumothorax. Pneumothorax has a clinical spectrum from asymptomatic patient to life-threatening situations. Diagnosis is usually made by clinical examination and imaging techniques. In our current work we focus on the treatment of penetrating trauma.
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Catamenial pneumothorax.
J Thorac Dis
PUBLISHED: 08-25-2014
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Catamenial pneumothorax (CP) is the most common form of thoracic endometriosis syndrome, which also includes catamenial hemothorax, catamenial hemoptysis, catamenial hemopneumothorax and endometriosis lung nodules, as well as some exceptional presentations. Usually onset of lung collapse is less than 72 hours after menstruation. Most commonly occurs in women aged 30-40 years, but has been diagnosed in young girls as early as 10 years of age and post menopausal women (exclusively in women of menstrual age) most with a history of pelvic endometriosis. Diagnosis can be hinted by high recurrence rates of lung collapse in a woman of reproductive age with endometriosis. Moreover; CA-125 is elevated. Video-assisted thoracoscopy or medical thoracoscopy is used for confirmation. In our current work we will present all aspects of CP from diagnosis to treatment.
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TGF-?-induced CD4+Foxp3+ T cells attenuate acute graft-versus-host disease by suppressing expansion and killing of effector CD8+ cells.
J. Immunol.
PUBLISHED: 08-25-2014
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The use of TGF-?-induced CD4(+)Foxp3(+) T cells (induced regulatory T cells [iTregs]) is an important prevention and treatment strategy in autoimmune diseases and other disorders. However, the potential use of iTregs as a treatment modality for acute graft-versus-host disease (aGVHD) has not been realized because they may be unstable and less suppressive in this disease. We restudied the ability of iTregs to prevent and treat aGVHD in two mouse models. Our results showed that, as long as an appropriate iTreg-generation protocol is used, these iTregs consistently displayed a potent ability to control aGVHD development and reduce mortality in the aGVHD animal models. iTreg infusion markedly suppressed the engraftment of donor CD8(+) cells and CD4(+) cells, the expression of granzyme A and B, the cytotoxic effect of donor CD8(+) cells, and the production of T cell cytokines in aGVHD. Therefore, we conclude that as long as the correct methods for generating iTregs are used, they can prevent and even treat aGVHD.
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Reduced responses to heroin-cue-induced craving in the dorsal striatum: effects of long-term methadone maintenance treatment.
Neurosci. Lett.
PUBLISHED: 08-23-2014
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Methadone maintenance treatment (MMT) is safe and effective for heroin addiction, but the neural basis of the length effects of long-term MMT on brain activity during craving in former heroin addicts is unclear. This study explored it by comparing the brain activations of heroin addicts with different length of MMT during pictorial presentation of heroin-related cue. Fifteen male former heroin addicts successfully treated by MMT less than 1 year (Group A), 15 matched patients with 2-3 year MMT (Group B) and 17 healthy controls underwent functional magnetic resonance imaging while heroin-related and neutral stimuli were present to them. Subjective cue-elicited craving was measured with visual analog scale before and after imaging. Then, partial correlation analysis to reveal the relationship between drug-related blood oxygen level dependent (BOLD) signal intensity and heroin or methadone use history. Finally, self-reported craving was not different between Group A and B before and after scanning. Compared with Group A, Group B had a significant reduced brain activity to heroin-related minus neural cues in the bilateral caudate. After controlling for the variable heroin use history, the drug-related BOLD signal intensity in the bilateral caudate was negatively correlated with MMT duration and total methadone consumption. When MMT history was controlled, the drug-related activity intensity in right caudate had a positive correlation with heroin daily dosage. Long-term MMT may improve heroin-craving response by modulating the impaired function in the bilateral dorsal striatum caused by former heroin use.
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Cloning and characterization of a Phragmites australis phytochelatin synthase (PaPCS) and achieving Cd tolerance in tall fescue.
PLoS ONE
PUBLISHED: 08-18-2014
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The production of phytochelatins (PCs) provides an important means for plants to achieve tolerance to cadmium (Cd) toxicity. A reed gene encoding PC synthase (PaPCS) was isolated and its function tested through its heterologous expression in a strain of yeast sensitive to Cd. Subsequently, the Cd sensitive and high biomass accumulating species tall fescue was transformed either with PaPCS or PaGCS (a glutamyl cysteine synthetase gene of reed) on their own (single transformants), or with both genes together in the same transgene cassette (double transformant). The single and double transformants showed greater Cd tolerance and accumulated more Cd and PC than wild type plants, and their Cd leaf/root ratio content was higher. The ranking in terms of Cd and PC content for the various transgenic lines was double transformants>PaGCS single transformants>PaPCS single transformants>wild type. Thus PaGCS appears to exert a greater influence than PaPCS over PC synthesis and Cd tolerance/accumulation. The double transformant has interesting potential for phytoremediation.
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Transbronchial lung biopsy and pneumothorax.
J Thorac Dis
PUBLISHED: 08-15-2014
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Mini-interventional procedures are used in the everyday clinical practice by pulmonary physicians and radiologists. Fine needle aspiration and biopsy forceps are the tools mostly used. During these procedures pneumothorax can occur and immediate treatment is necessary. In our current work, we will focus on minimal invasive techniques for biopsy and pneumothorax treatment.
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Pneumothorax: observation.
J Thorac Dis
PUBLISHED: 08-15-2014
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Pneumothorax based on the cause, it can be divided into two large categories; primary and secondary. The staging of pneumothorax plays a crucial role for treatment. Currently both thoracic surgeons and pulmonary physicians can handle efficiently treatment. Pulmonary physicians with the minimally medical thoracoscopy while thoracic surgeons with a more extensive intervention. Experience defines the outcome in most situations and not the method. In our current work we will present data regarding the observation of pneumothorax from a panel of experts.
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Ten Years' Experience for Hypospadias Repair: Combined Buccal Mucosa Graft and Local Flap for Urethral Reconstruction.
Urol. Int.
PUBLISHED: 08-13-2014
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Objectives: To share our experience in hypospadias repair and discuss the clinical implications of our method, which consists of a combined buccal mucosa graft and local flap for urethroplasty. Patients and Methods: 1,394 cases (median age 11.3 years, age range 5 months to 53 years) of hypospadias which were repaired using our method between July 2000 and December 2010 in our department were included in this study. The patients who had a short penis or did not have chordee were excluded from the data. 588 cases (42.2%) had previously undergone surgery in other hospitals but failed; 806 (57.8%) cases had undergone the first treatment in our department. Results: Of the 806 cases which had undergone the first treatment in our department, we successfully reconstructed the urethra for 747 patients (92.3%), and 59 patients had complications (7.7%); of the 588 cases which had previously undergone surgery but failed, we successfully reconstructed the urethra for 522 patients (88.8%), and 66 patients had complications (11.2%). The most common complication was urethra fistula (70; 5%); other complications include necrosis of the skin flap and infection resulting in wound disruption (19; 1.4%), urethral diverticula (11; 0.8%) and urethral stricture (25; 1.8%). Conclusions: Our method appears to be a safe, simple and satisfactory surgical procedure and can provide relatively enough tissue to reconstruct the urethra with a higher success rate. © 2014 S. Karger AG, Basel.
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Characterization and flocculation mechanism of an alkali-activated polysaccharide flocculant from Arthrobacter sp. B4.
Bioresour. Technol.
PUBLISHED: 08-12-2014
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The characterization and flocculation mechanism of a bioflocculant produced by Arthrobacter sp. B4 were investigated. The bioflocculant's active ingredient was a polysaccharide (B4-PS) that consisted of three main fractions corresponding to the molecular weights of approximately 3.97×10(4)Da, 6.84×10(3)Da and 5.9×10(6)Da, respectively. These fractions were composed of galactose, glucose, mannose and glucuronic acid. Flocculation experiments showed that B4-PS could spontaneously flocculate in the presence of Ca(2+) ions at a high pH (>12.0), followed by the pH reduction to ?6.0. The self-flocculation of B4-PS may be mediated by ionization and charge neutralization mechanism. Furthermore, B4-PS exhibited excellent capabilities for pollutant removal and pH reduction in alkaline wastewater. These data suggest B4-PS may be a promising tool for use in industrial alkaline wastewater pretreatment.
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Optimization of nebulized delivery of linezolid, daptomycin, and vancomycin aerosol.
Drug Des Devel Ther
PUBLISHED: 08-12-2014
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At this time, several antibiotics have been investigated as possibilities for aerosol administration, but local therapy has been found to be more efficient in several diseases.
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Approach of the treatment for pneumothorax.
J Thorac Dis
PUBLISHED: 08-09-2014
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Pneumothorax can occur in several situations such as; chronic obstructive pulmonary disease (COPD) where emphysema is observed or due to a biopsy for malignancy suspicion. In any case it is a dangerous situation that requires immediate attention and treatment. Pneumothorax can be divided in primary and secondary. Staging of pneumothorax is also very important. In our current editorial we summarize etiology and treatment of pneumothorax from a panel of pulmonary physicians, oncologists and thoracic surgeons.
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One- and two-dimensional Cd(II) coordination polymers constructed from 2-(2-methyl-1H-benzimidazol-1-yl)acetate ligands.
Acta Crystallogr C Struct Chem
PUBLISHED: 08-05-2014
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The one- and two-dimensional polymorphic cadmium polycarboxylate coordination polymers, catena-poly[bis[?2-2-(2-methyl-1H-benzimidazol-1-yl)acetato-?(3)N(3):O,O']cadmium(II)], [Cd(C10H9N2O2)2]n, and poly[bis[?2-2-(2-methyl-1H-benzimidazol-1-yl)acetato-?(3)N(3):O,O']cadmium(II)], also [Cd(C10H9N2O2)2]n, were prepared under solvothermal conditions. In each structure, each Cd(II) atom is coordinated by four O atoms and two N atoms from four different ligands. In the former structure, two crystallographically independent Cd(II) atoms are located on twofold symmetry axes and doubly bridged in a ?2-N:O,O'-mode by the ligands into correspondingly independent chains that run in the [100] and [010] directions. Chains containing crystallographically related Cd(II) atoms are linked into sheets via ?-? stacking interactions. Sheets containing one of the distinct types of Cd(II) atom are stacked perpendicular to [001] and alternate with sheets containing the other type of Cd(II) atom. The second complex is a two-dimensional homometallic Cd(II) (4,4) net structure in which each Cd(II) atom is singly bridged to four neighbouring Cd(II) atoms by four ligands also acting in a ?2-N:O,O'-mode. A square-grid network results and the three-dimensional supramolecular framework is completed by ?-? stacking interactions between the aromatic ring systems.
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A new three-dimensional anionic cadmium(II) dicyanamide network.
Acta Crystallogr C Struct Chem
PUBLISHED: 08-01-2014
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A new cadmium dicyanamide complex, poly[tetramethylphosphonium [?-chlorido-di-?-dicyanamido-?(4)N(1):N(5)-cadmium(II)]], [(CH3)4P][Cd(NCNCN)2Cl], was synthesized by the reaction of tetramethylphosphonium chloride, cadmium nitrate tetrahydrate and sodium dicyanamide in aqueous solution. In the crystal structure, each Cd(II) atom is octahedrally coordinated by four terminal N atoms from four anionic dicyanamide (dca) ligands and by two chloride ligands. The dicyanamide ligands play two different roles in the building up of the structure; one role results in the formation of [Cd(dca)Cl]2 building blocks, while the other links the building blocks into a three-dimensional structure. The anionic framework exhibits a solvent-accessible void of 673.8?Å(3), amounting to 47.44% of the total unit-cell volume. The cavities in the network are occupied by pairs of tetramethylphosphonium cations.
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Gene Expression Analysis of the 26S Proteasome Subunit PSMB4 Reveals Significant Upregulation, Different Expression and Association with Proliferation in Human Pulmonary Neuroendocrine Tumours.
J Cancer
PUBLISHED: 08-01-2014
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Proteasomal subunit PSMB4 was suggested to be a survival gene in an animal model of hepatocellular carcinoma and in glioblastoma cell lines. In pulmonary adenocarcinoma, a high expression of these genes was found to be associated with poor differentiation and survival. This study investigates the gene expression levels of 26S proteasome subunits in human pulmonary neuroendocrine tumours including typical (TC) and atypical (AC) carcinoid tumours as well as small cell (SCLC) and large cell (LCNEC) neuroendocrine carcinomas.
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DAXAS and COPD Correlation with Cancer.
J Cancer
PUBLISHED: 08-01-2014
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A new drug for chronic obstructive pulmonary disease has been recently added as a treatment for certain patients. However, new evidences indicate that there might be a connection with lung cancer. It is known that smoking is a major factor that induces chronic pulmonary disease and smoking is associated with lung cancer. The level of connection between phosphodiesterase (PDE)-4 inhibitors and lung cancer will be discussed based on current studies. A comment will be made whether lung cancer is induced to patients receiving phosphodiesterase (PDE)-4 inhibitors from the drug or former smoking habit.
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Evidence of Stranski-Krastanov growth at the initial stage of atmospheric water condensation.
Nat Commun
PUBLISHED: 07-29-2014
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The precipitation products (rain, snow and so on) of atmospheric water vapour are widely prevalent, and yet the map of its initial stage at a surface is still unclear. Here we investigate the condensation of water vapour occurring in both the hydrophobic-hydrophilic interface (graphene/mica) and the hydrophilic-hydrophilic interface (MoS2/mica) by in situ thermally controlled atomic force microscopy. By monitoring the dynamic dewetting/rewetting transitions process, the ice-like water adlayers, at the hydrophobic-hydrophilic interface and not at the hydrophilic-hydrophilic interface, stacked on top of each other up to three ice-Ih layers (each of height 3.7 ± 0.2 Å), and the transition from layers to droplets was directly visualized experimentally. Compared with molecular dynamics simulation, the Stranski-Krastanov growth model is better suited to describe the whole water condensation process at the hydrophobic-hydrophilic interface. The initial stage of the hydrometeor is rationalized, which potentially can be utilized for understanding the boundary condition for water transport and the aqueous interfacial chemistry.
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Higher vaspin levels in subjects with obesity and type 2 diabetes mellitus: a meta-analysis.
Diabetes Res. Clin. Pract.
PUBLISHED: 07-27-2014
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Visceral adipose tissue-derived serpin (vaspin) was identified as a new adipocytokine. Many studies reported vaspin concentrations in obese subjects and type 2 diabetes mellitus (T2DM) patients. However, large variation in levels of vaspin seen in different studies may be attributable to differences of sample size. The aim of this study is to establish an accurate confidence interval of vaspin levels in obese subjects and T2DM patients using a large-scale meta-analysis.
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Effects of an adenoviral vector containing a suicide gene fusion on growth characteristics of breast cancer cells.
Mol Med Rep
PUBLISHED: 07-22-2014
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The herpes simplex virus thymidine kinase/ganciclovir (HSV?TK/GCV) and the cytosine deaminase/5?fluorocytosine (CD/5?FC) systems have been widely applied in suicide gene therapy for cancer. Although suicide gene therapy has been successfully used in vitro and in vivo studies, the number of studies on the effects of recombinant adenoviruses (Ads) containing suicide genes on target cancer cells is limited. The aim of this study was to examine whether recombinant Ads containing the CD/TK fusion gene affect cell proliferation of breast cancer cells in vitro. In the present study, we explored the use of a recombinant adenoviral vector to deliver the CD/TK fusion gene to the breast cancer cell line MCF?7. We found that the recombinant adenoviral vector efficiently infected MCF?7 cells. Western blot analysis revealed that CD and TK proteins are expressed in the infected cells. The infected breast cancer cells did not show any significant changes in morphology, ultrastructure, cell growth, and cell?cycle distribution compared to the uninfected cells. This study revealed that the Ad?vascular endothelial growth factor promoter (VEGFp)?CD/TK vector is non?toxic to MCF?7 cells at the appropriate titer. Our results indicate that it is feasible to use a recombinant adenoviral vector containing the CD/TK fusion gene in suicide gene therapy to target breast cancer cells.
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Taxonomy of the Nacerdes (Xanthochroa) carniolica species-group from China (Coleoptera, Oedemeridae, Nacerdini).
Zookeys
PUBLISHED: 07-17-2014
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This paper deals with a species-group carniolica of the genus Nacerdes from China. This species-group has seven known species/subspecies in the world and two of them are known from China. Nacerdes (Xanthochroa) arcuata sp. n. is a new species belonging to carniolica group. The species were collected from Anhui (Eastern China, 30°02'17.37"N, 118°50'1.72"E). A key to the species of the species-group from China is given along with a distribution map.
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Probing the Anticancer Mechanism of (-)-Ainsliatrimer?A through Diverted Total Synthesis and Bioorthogonal Ligation.
Angew. Chem. Int. Ed. Engl.
PUBLISHED: 07-15-2014
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Herein, we report an efficient approach for exploring the novel anticancer mechanism of (-)-ainsliatrimer?A, a structurally complex and unique trimeric sesquiterpenoid, through a combined strategy of diverted total synthesis (DTS) and bioorthogonal ligation (TQ ligation), which allowed us to visualize the subcellular localization of this natural product in live cells. Further biochemical studies facilitated by pretarget imaging revealed that PPAR?, a nucleus receptor, was a functional cellular target of ainsliatrimer?A. We also confirmed that the anticancer activity of ainsliatrimer?A was caused by the activation of PPAR?.
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On K-peptide length in composition vector phylogeny of prokaryotes.
Comput Biol Chem
PUBLISHED: 07-11-2014
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Using an enlarged alphabet of K-tuples is the way to carry out alignment-free comparison of genomes in the composition vector (CV) approach to prokaryotic phylogeny. We summarize the known aspects concerning the choice of K and examine the results of using CVs with subtraction of a statistical background for K=3-9 and using raw CVs without subtraction for K=1-12. The criterion for evaluation consists in direct comparison with taxonomy. For prokaryotes the best performances are obtained for K=5 and 6 with subtraction and for K=11, 12 or even more without subtraction. In general, CVs with subtractions are slightly better and less CPU consuming, but CVs without subtraction may provide complementary information.
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Effects of pamidronate disodium on the loss of osteoarthritic subchondral bone and the expression of cartilaginous and subchondral osteoprotegerin and RANKL in rabbits.
BMC Musculoskelet Disord
PUBLISHED: 07-10-2014
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Osteoarthritis (OA) is a major health problem in the increasingly elderly population. Therefore, it is crucial to prevent and treat OA at an early stage. The present study investigated whether pamidronate disodium (PAM), a bone-loss inhibitor, can significantly prevent or reverse the progression of early anterior cruciate ligament transection (ACLT)-induced OA. Whether therapeutic intervention is associated with regulation of the expression of osteoprotegerin (OPG), receptor activator of nuclear factor-kappaB ligand (RANKL), metalloproteinase-9 (MMP-9) or Toll-like receptor-4 (TLR-4) in cartilage and/or subchondral bone was also investigated.
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Ionic liquid pretreatment to increase succinic acid production from lignocellulosic biomass.
Bioresour. Technol.
PUBLISHED: 07-10-2014
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In this study, pinewood and corn stover pretreated with the ionic liquid (IL) 1-allyl-3-methylimidazolium chloride (AmimCl) were used as a feedstock for succinic acid production. Results reveal that 5% (v/v) AmimCl inhibited bacterial growth, whereas 0.01% (v/v) AmimCl inhibited succinic acid production. AmimCl was effective in extracting cellulose from pinewood and in degrading pinewood into a uniform pulp, as revealed by scanning electron microscopy (SEM). The rate of enzymatic hydrolysis of pinewood extract reached 72.16%. The combinations of AmimCl pretreatment with steam explosion or with hot compressed water were effective in treating corn stover, whereas AmimCl treatment alone did not result in a significant improvement. Pinewood extract produced 20.7g/L succinic acid with an average yield of 0.37g per gram of biomass. Workflow calculations indicated pine wood pretreated with IL has a theoretical yield of succinic acid of 57.1%. IL pretreatment led to increase in succinic acid yields.
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A novel biosensor-based microarray assay for the visualized detection of CYP2C19 ?2, ?3, ?4 and ?5 polymorphisms.
Clin. Chem. Lab. Med.
PUBLISHED: 07-07-2014
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Abstract Background: A number of studies have indicated that the conversion of clopidogrel to its active metabolite is reduced in patients who carry the CYP2C19 *2, *3, *4 or *5 loss-of-function allele, resulting in decreased response of platelet to clopidogrel treatment and worse cardiovascular outcome. The aim of this study was to develop a novel biosensor-based microarray to visually detect CYP2C19 polymorphisms. Methods: The target DNA was amplified from regions flanking the respective alleles using 5'-biotinylated reverse primer, and plasmids were prepared for the respective alleles. High stringency reversed hybridization, horseradish peroxidase-labeled streptavidin reaction, and color development, with multiple washes in different steps, were carried out and the results were recorded with an optical camera. The gene chips were tested for specificity, detection limit, intra- and inter-batch variations using the constructed plasmids. Finally, 88 clinical samples were assayed with this microarray as well as direct sequencing. Results: The results could be seen with the naked eye. Concordance tests indicated that for alleles *2, *3, *4, and *5, the ? values between this assay and plasmids all reached 1.000. The detection limit was 5×102 cells/mL. Concordance test between direct sequencing and the microarray assay using 88 clinical samples gave rise to the ? value of 0.983, and p<0.01, indicating very high concordance. Conclusions: This novel biosensor-based microarray assay can amplify the signal in situ so that it can be detected by simple instruments or even the naked eyes. It is promising for clinical application in hospital laboratories.
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Experimentation with aerosol bonsetan, pirfenidone, treprostinil and sidenafil.
J Thorac Dis
PUBLISHED: 07-02-2014
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Pulmonary hypertension (PH) has been identified either as a symptom or a primary entity. Several drugs are already on the market and other are being investigated. Idiopathic pulmonary fibrosis (IPF) is also a disease were several drugs are being investigated.
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Significantly Decreased and More Variable Expression of Major CYPs and UGTs in Liver Microsomes Prepared from HBV-Positive Human Hepatocellular Carcinoma and Matched Pericarcinomatous Tissues Determined Using an Isotope Label-free UPLC-MS/MS Method.
Pharm. Res.
PUBLISHED: 07-02-2014
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To determine the liver expression of cytochrome P450 (CYPs) and uridine 5'-diphosphate-glucuronosyltransferases (UGTs), the major phase I and II metabolism enzymes responsible for clearance and detoxification of drugs, xenobiotic and endogenous substances.
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MT1-MMP is not a good prognosticator of cancer survival: evidence from 11 studies.
Tumour Biol.
PUBLISHED: 06-27-2014
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MT1-MMP exhibits diverse expressions in patients with cancer and could be considered as potential prognostic biomarker of cancer. We performed a meta-analysis aiming to provide more sufficient evidence that MT1-MMP expression is associated with poor overall survival in several types of cancers. We systematically searched the studies from databases and carefully identified based on eligibility criteria. The association between MT1-MMP expression and overall survival in cancers was estimated using Review Manager. A total of 11 literatures which included 1,918 cancer patients were combined in the final analysis. Meta-analysis revealed that MT1-MMP overexpression was associated with an unfavorable overall survival and the pooled hazard ratio (HR) and corresponding 95 % confidence interval (CI) was 2.46 (95 % CI 1.75-3.47). From subgroup analyses, we identified that MT1-MMP was an independent prognostic factor for lung cancer and gastric cancer, and HRs (95 % CI) were 3.73 (95 % CI 2.67-5.21) and 2.46 (95 % CI 1.69-3.59), respectively. In conclusion, MT1-MMP is a potential prognostic factor in human cancers.
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Intraportal infusion of ghrelin could inhibit glucose-stimulated GLP-1 secretion by enteric neural net in Wistar rat.
Biomed Res Int
PUBLISHED: 06-20-2014
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As a regulator of food intake and energy metabolism, the role of ghrelin in glucose metabolism is still not fully understood. In this study, we determined the in vivo effect of ghrelin on incretin effect. We demonstrated that ghrelin inhibited the glucose-stimulated release of glucagon-like peptide-1 (GLP-1) when infused into the portal vein of Wistar rat. Hepatic vagotomy diminished the inhibitory effect of ghrelin on glucose-stimulated GLP-1 secretion. In addition, phentolamine, a nonselective ? receptor antagonist, could recover the decrease of GLP-1 release induced by ghrelin infusion. Pralmorelin (an artificial growth hormone release peptide) infusion into the portal vein could also inhibit the glucose-stimulated release of GLP-1. And growth hormone secretagogue receptor antagonist, [D-lys3]-GHRP-6, infusion showed comparable increases of glucose stimulated GLP-1 release compared to ghrelin infusion into the portal vein. The data showed that intraportal infusion of ghrelin exerted an inhibitory effect on GLP-1 secretion through growth hormone secretagogue receptor 1? (GHS1? receptor), which indicated that the downregulation of ghrelin secretion after food intake was necessary for incretin effect. Furthermore, our results suggested that the enteric neural net involved hepatic vagal nerve and sympathetic nerve mediated inhibition effect of ghrelin on incretin effect.
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Effect of occlusal hypofunction and its recovery on the three-dimensional architecture of mandibular alveolar bone in growing rats.
J. Surg. Res.
PUBLISHED: 06-19-2014
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Normal occlusion is very important for physiological structure of mandible. However, the details of influences of occlusal hypofunction and its recovery on the three-dimensional architecture of mandibular alveolar bone in growing rats are still lacking.
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What is Visualize?

JoVE Visualize is a tool created to match the last 5 years of PubMed publications to methods in JoVE's video library.

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We use abstracts found on PubMed and match them to JoVE videos to create a list of 10 to 30 related methods videos.

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In developing our video relationships, we compare around 5 million PubMed articles to our library of over 4,500 methods videos. In some cases the language used in the PubMed abstracts makes matching that content to a JoVE video difficult. In other cases, there happens not to be any content in our video library that is relevant to the topic of a given abstract. In these cases, our algorithms are trying their best to display videos with relevant content, which can sometimes result in matched videos with only a slight relation.