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Find video protocols related to scientific articles indexed in Pubmed.
[Prevalence status for smokers of undergraduates in Huaihua and the influential factors].
Zhong Nan Da Xue Xue Bao Yi Xue Ban
PUBLISHED: 10-31-2014
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To investigate the prevalence status and the influential factors for smokers of undergraduates in Huaihua.
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[Clinical pathological features of small renal cell cancer: a single-center experience on 1 267 cases].
Beijing Da Xue Xue Bao
PUBLISHED: 10-22-2014
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To study the characteristics of histopathologic features of small renal carcinoma.
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lincRNA-RoR and miR-145 Regulate Invasion in Triple-negative Breast Cancer via Targeting ARF6.
Mol. Cancer Res.
PUBLISHED: 09-26-2014
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Triple-negative (ER-, HER2-, PR-) breast cancer (TNBC) is an aggressive disease with a poor prognosis with no available molecularly targeted therapy. Silencing of microRNA-145 (miR-145) may be a defining marker of TNBC based on molecular profiling and deep sequencing. Therefore, the molecular mechanism behind miR-145 down-regulation in TNBC was examined. Overexpression of the long non-coding RNA, lincRNA-RoR, functions as a competitive endogenous RNA sponge in TNBC. Interestingly, lincRNA-RoR is dramatically upregulated in TNBC and in metastatic disease and knockdown restores miR-145 expression. Previous reports suggest that miR-145 has growth suppressive activity in some breast cancers; however, the current data in TNBC indicates that miR-145 does not impact proliferation or apoptosis but instead, miR-145 regulates tumor cell invasion. Investigation of miR-145 regulated pathways involved in tumor invasion revealed a novel target, the small GTPase ADP-ribosylation factor 6 (Arf6). Subsequent analysis demonstrated that ARF6, a known regulator of breast tumor cell invasion, is dramatically upregulated in TNBC and in breast tumor metastasis. Mechanistically, ARF6 regulates E-cadherin localization and impacts cell-cell adhesion. These results reveal a lincRNA-ROR/miR-145/ARF6 pathway that regulates invasion in TNBCs. Implications: The lincRNA-RoR/miR-145/ARF6 pathway is critical to TNBC metastasis and could serve as biomarkers or therapeutic targets for improving survival.
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NRF2/Long Noncoding RNA ROR Signaling Regulates Mammary Stem Cell Expansion and Protects against Estrogen Genotoxicity.
J. Biol. Chem.
PUBLISHED: 09-17-2014
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Long noncoding RNAs (lncRNAs) have emerged as key regulators of gene expression in embryonic stem cell (ESC) self-renewal and differentiation. In ESCs, lncRNAs are regulated at the genetic level via transcription factor binding to lncRNA gene promoters. Here we demonstrate that the key cytoprotective transcription factor NRF2 controls lncRNA expression in mammary stem cells. By profiling lncRNAs in wild-type and NRF2 knockdown mammary stem cells, we demonstrate that the lncRNA ROR, a regulator of embryonic stem cell pluripotency, is overexpressed upon NRF2 knockdown. We performed promoter analyses and examined predicted NRF2 binding elements in the ROR promoter using luciferase reporter constructs of a ROR promoter deletion series. Our studies revealed that NRF2 binds to two specific NRF2 response elements flanking the ROR promoter and that these two NRF2 response elements are equally important to suppress ROR transcription. In addition, we identified associated H3K27me3 chromatin modification and EZH2 binding at the ROR promoter that was dependent on NRF2 binding. We observed that NRF2 knockdown or ROR overexpression leads to increased stem cell self-renewal in mammary stem cells. Furthermore, we demonstrate Nrf2 regulation of the mammary stem cell population in vivo. These observations provide further evidence for the critical role of NRF2 in maintaining normal stem cell subpopulations in mammary epithelium.
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[Analysis of different parts and tissues of Panax Notoginseng by Fourier transform infrared spectroscopy].
Guang Pu Xue Yu Guang Pu Fen Xi
PUBLISHED: 09-12-2014
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The techniques of Fourier transform infrared (FTIR) spectroscopy were applied to analyze the different parts and tissues of Panax Notoginseng (Sanqi, SQ), i.e. rhizome, main root, rootlet, fibrous root, xylem, cambium, phloem and epidermis. Both the FTIR spectra and second derivative spectra of these various parts and tissues of SQ samples were found to be similar. Their dominant component is starch resulting from the characteristic peaks of starch observed at 3 400, 2 930, 1 645, 1 155, 1,080 and 1,020 cm(-1) on the spectra of all these SQ samples. However, the varieties of peaks were found on the spectra among these specific samples. The rhizome contains more saponins than others on the basis of the largest ratio of the peak intensity at 1,077 cm(-1) to that at 1,152 cm(-1). The peaks located at 1 317 and 780 cm(-1) on the FTIR spectra of the rhizome and its epidermis indicate that the two parts of SQ samples contain large amount of calcium oxalate, and its content in the latter is relative larger than that in former. The fibrous root contains much amount of nitrate owing to the obvious characteristic peaks at 1 384 and 831 cm(-1). For the difference among the various tissues of SQ samples, the peaks at 2,926, 2,854 and 1,740 cm(-1) on the FTIR spectra of epidermis is the strongest among the various tissues of main root indicating the largest amount of esters in epidermis. Protein was also found in the cambium of the main root based on the relative strong peaks of amide I and II band at 1,641 and 1,541 cm(-1), respectively. The results indicate that FTIR spectra with its second derivative spectra can show the characteristic of the various parts and tissues of SQ samples in both the holistic chemical constituents and specific chemical components, including organic macromolecule compounds and small inorganic molecule compounds. FTIR spectroscopy is a useful analytical method for the genuine and rapid identification and quality assessment of SQ samples.
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Examining estrogen regulation of cancer stem cells through multicolor lineage tracing.
Methods Mol. Biol.
PUBLISHED: 09-04-2014
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We have designed a dual-color fluorescent reporter that can track microRNA expression in vitro, which can be used for lineage tracing experiments. We have used this system to track miR-140 promoter activity in breast cancer cells and to follow the impact of estrogen signaling in cancer stem cell subpopulations.
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MicroRNA-191 promotes pancreatic cancer progression by targeting USP10.
Tumour Biol.
PUBLISHED: 08-29-2014
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Recent studies have shown that microRNAs, a class of small and noncoding RNA molecules, play crucial roles in the initiation and progression of pancreatic cancer. In the present study, the expression and roles of miR-191 were investigated. Through both gain-of function and loss-of function experiments, a pro-oncogenic function of miR-191 was demonstrated. At the molecular level, bioinformatic prediction, luciferase, and protein expression analysis suggested that miR-191 could inhibit protein levels of UPS10, which suppressed the proliferation and growth of cancer cells through stabilizing P53 protein. Collectively, these data suggest that miR-191 could promote pancreatic cancer progression through targeting USP10, implicating a novel mechanism for the tumorigenesis.
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Surfactant-assisted synthesis of Fe2O3 nanoparticles and F-doped carbon modification toward an improved Fe3O4@CFx/LiNi0.5Mn1.5O4 battery.
ACS Appl Mater Interfaces
PUBLISHED: 08-27-2014
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A simple surfactant-assisted reflux method was used in this study for the synthesis of cocklebur-shaped Fe2O3 nanoparticles (NPs). With this strategy, a series of nanostructured Fe2O3 NPs with a size distribution ranging from 20 to 120 nm and a tunable surface area were readily controlled by varying reflux temperature and the type of surfactant. Surfactants such as cetyltrimethylammonium bromide (CTAB), polyvinylpyrrolidone (PVP), poly(ethylene glycol)-block-poly(propylene glycol)-block-poly(ethylene glycol) (F127) and sodium dodecyl benzenesulfonate (SDBS) were used to achieve large-scale synthesis of uniform Fe2O3 NPs with a relatively low cost. A new composite of Fe3O4@CFx was prepared by coating the primary Fe2O3 NPs with a layer of F-doped carbon (CFx) with a one-step carbonization process. The Fe3O4@CFx composite was utilized as the anode in a lithium ion battery and exhibited a high reversible capacity of 900 mAh g(-1) at a current density of 100 mA g(-1) over 100 cycles with 95% capacity retention. In addition, a new Fe3O4@CFx/LiNi(0.5)Mn(1.5)O4 battery with a high energy density of 371 Wh kg(-1) (vs cathode) was successfully assembled, and more than 300 cycles were easily completed with 66.8% capacity retention at 100 mA g(-1). Even cycled at the high temperature of 45 °C, this full cell also exhibited a relatively high capacity of 91.6 mAh g(-1) (vs cathode) at 100 mA g(-1) and retained 54.6% of its reversible capacity over 50 cycles. Introducing CFx chemicals to modify metal oxide anodes and/or any other cathode is of great interest for advanced energy storage and conversion devices.
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Synergy of urokinase?type plasminogen activator receptor isomer (D1D2) and integrin ?5?1 causes malignant transformation of hepatic cells and the occurrence of liver cancer.
Mol Med Rep
PUBLISHED: 08-20-2014
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The aim of the present study was to investigate the correlations and possible synergy among the urokinase?type plasminogen activator receptor (uPAR) isomer D1D2 and integrin ?5?1 expression levels, malignant transformation in hepatic cells and the occurrence of liver cancer. The expression site and concentration of uPAR (D1D2) were analyzed using polymerase chain reaction and in situ hybridization at the gene level in 60 samples of hepatocellular carcinoma (HCC) tissues, 60 samples of para?carcinoma tissues and 25 samples of normal liver tissues. The mRNA levels of uPAR (D1D2) and integrin ?5?1 were markedly increased para?carcinoma tissue and liver cancer tissue as compared with those in normal tissue. The grey values of the three groups were significantly different (P<0.05). In situ hybridization revealed that the expression levels of uPAR (D1D2) and integrin ?5?1 in the cytoplasm and the positive rate of the two molecules in the HCC tissue were significantly higher than those in the para-carcinoma and normal liver tissues, and the expression levels were positively correlated (rs1=0.257, P<0.05; rs2=0.261, P<0.05). The results suggested that uPAR (D1D2) mRNA overexpression may be due to changes in the conformation of the uPAR isomer. Synergy of uPAR (D1D2) mRNA and integrin ?5?1 interaction may result in abnormal signal transduction in liver cells and ultimately liver cell abnormal clonal hyperplasia and malignant transformation.
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[A prospective randomized control study: new rigid cystoscopy technology to improve patients' comfort].
Beijing Da Xue Xue Bao
PUBLISHED: 08-19-2014
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To determine whether the extruded irrigation solution bag during passage of rigid cystoscope will reduce the patient's discomfort.
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[Technique of renal pedicle control in transperitoneal laparoscopic nephrectomy: experience of 191 cases by a single surgeon].
Beijing Da Xue Xue Bao
PUBLISHED: 08-19-2014
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To explore the technique of vascular control in transperitoneal laparoscopic nephrectomy.
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[Long-term outcome of isolated local recurrence following radical nephrectomy].
Beijing Da Xue Xue Bao
PUBLISHED: 08-19-2014
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To characterize the long-term outcome of surgical extirpation for local recurrence after radical nephrectomy of renal cell carcinoma (RCC) and identify prognostic factors for locally recurrent RCC.
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[Characteristics of lymph node metastasis in patients undergoing radical cystectomy for bladder cancer: a retrospective single-center study of 522 cases].
Beijing Da Xue Xue Bao
PUBLISHED: 08-19-2014
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To investigate the characteristics and related influence factors of lymph node metastases in patients undergoing radical cystectomy and lymph node dissection for bladder cancer.
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[Transperitoneal laparoscopic ureteral reimplantation with extracorporeal tailoring and direct nipple ureteroneocystostomy for adult obstructive megaureter].
Beijing Da Xue Xue Bao
PUBLISHED: 08-19-2014
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This paper focuses on a novel modified technique about the treatment of adult obstructed megaureter by the transperitoneal laparoscopic procedure. With the improvement of the laparoscopic surgery, many urological surgeries can be safely and effectually performed by laparoscopic approach. The previously reported laparoscopic methods for treatment of adult obstructed megaureter were complex and time-consuming. To simplify the method, we modified the laparoscopic approach based on the previous methods. The innovative points of our novel technique are the extracorporeal tailoring of ureter and nipple ureteroneocystostomy. By this modified procedure, the time of operation can be obviously reduced while the procedure is effective. We hope this modified procedure will be accepted by more urologists.
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Laparoscopic versus Open Partial Nephrectomy for Multilocular Cystic Renal Cell Carcinoma: A Direct Comparison Based on Single-Center Experience.
Urol. Int.
PUBLISHED: 08-07-2014
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Objectives: To compare the treatment of laparoscopic (LPN) versus open partial nephrectomy (OPN) in patients with multilocular cystic renal cell carcinoma (MCRCC). Methods: Thirty-seven patients diagnosed with MCRCC were reviewed retrospectively between January 2007 and January 2013 at our institution. They were divided into two groups: group 1 (LPN) consisted of 19 patients (51.4%) and group 2 (OPN) of 18 patients (48.6%). RENAL and the Preoperative Aspects and Dimensions Used for an Anatomical classification were applied to predict perioperative complications, which were graded based on the Clavien-Dindo classification. Results: The two groups were comparable with regard to all of the patients' baseline characteristics. In group 1, the mean operative time was 142.1 min, including the mean warm ischemia time (WIT) of 32.6 min; the mean estimated blood loss (EBL) was 96.1 ml, the mean retroperitoneal drainage lasted 3.6 days, and the mean postoperative hospital stay was 5.3 days. In group 2, the figures were 126.6 and 23.5 min, 223.3 ml, and 4.6 and 8.7 days, respectively. The differences in WIT, EBL, drainage days and hospitalization were statistically significant between both groups (p < 0.05). No recurrence or new lesions occurred in these patients during a mean follow-up of 37.8 months. Conclusions: Our single-center experience suggests that although it remains technically complex, demanding and challenging for MCRCC, LPN can still induce favorable perioperative results and survival rates in MCRCC are comparable with OPN. © 2014 S. Karger AG, Basel.
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[Special impact of supramolecular chemistry on Chinese medicine theories].
Zhongguo Zhong Yao Za Zhi
PUBLISHED: 07-22-2014
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The paper aimed to elucidate the specific impact of supramolecular chemistry on the Chinese medicine theories (CMT) in their modernization, after had summarized up the research status of supramolecular chemistry and analyzed the possible supramolecular forms of Chinese medicine (CM), as well as considered the problems in modernization of CM theories. On comparison of the classical chemistry that delt with chemical bonds among atoms, the supramolecular chemistry was rather concerned with varietes of weak noncovalent bonds intermolecules, and reflected the macro-apparent chemical properties of each molecules, and was the most appropriate chemical theories to explain the CMT and microcosmic materials. The molecules in the human body and Chinese material medica (CMM) formed supramolecules by way of self-assembly, self-organization, self-recognition and self-replication, with themselves or with complexation, composition, chelation, inclusion, neutralization etc. Meridian and Zang-fu viscera in CMT might be a space channel structure continuously consisted of unique molecules cavity that was imprinted with the supramolecularly template inside and outside of cells, through which the molecules in CMM interacted with the meridian and Zang-fu viscera. When small molecules in human body imprinted with macromolecules in meridian and Zang-fu viscera, in other words, they migrated along within imprinting channels of meridian and Zang-fu viscera on behavior of "Qi chromatography" impulsed by the heart beat, finally showed up on macroscopic the anisotropy of tissue and organ, as described namely as visceral manifestation in Chinese medical science. When small molecules in CMM interacted with imprinting channel on meridian and Zang-fu viscera, the natural properties and efficacy regularities of CMM was reflected on macroscopic. Therefore, the special representation forms of basic CMT is based on the macroscopic expression of "Qi chromatography" abided by imprinting effect regularities, and on whether the imprinted template of small molecules matched with cavity template of macromolecules in meridian and Zang-fu viscera, only is the adequate representation of supramolecular chemistry for them. The CMM materials is the mixture including single molecules and supramolecules. The compatibility for CM prescriptions can significantly change the function rules. Therefore in the study of basic CMT, we should pay special attention to the laws of supramolecular chemistry. It is the most essential differences of the CMT from the modern medicine which established by the laws of single molecular theories.
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Noninvasive in-vehicle alcohol detection with wavelength-modulated differential photothermal radiometry.
Biomed Opt Express
PUBLISHED: 07-01-2014
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This study describes the potential of wavelength-modulated differential photothermal radiometry (WM-DPTR) for non-invasive in-vehicle alcohol detection which can be of great importance in reducing alcohol-impaired driving. Ethanol content in the range of concern, 0-100 blood alcohol concentration (BAC) in water phantoms and blood serum diffused in human skin in vitro were measured with high sensitivity. The results show that the WM-DPTR system can be optimized for alcohol detection with the combination of two sensitivity-tuning parameters, amplitude ratio R and phase shift ?P. WM-DPTR has demonstrated the potential to be developed into a portable alcohol ignition interlock biosensor that could be fitted as a universal accessory in vehicles.
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Association of STAT4 gene rs7574865G > T polymorphism with ulcerative colitis risk: evidence from 1532 cases and 3786 controls.
Arch Med Sci
PUBLISHED: 06-27-2014
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Several studies have reported the relationship between the STAT4 rs7574865G > T polymorphism as a susceptibility factor to ulcerative colitis (UC). However, the results have been controversial. Therefore, we conducted this meta-analysis to obtain the most reliable estimate of the association.
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Penifupyrone, a new cytotoxic funicone derivative from the endophytic fungus Penicillium sp. HSZ-43.
Nat. Prod. Res.
PUBLISHED: 06-18-2014
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Penifupyrone (1), a new funicone derivative, has been isolated from the endophytic fungus Penicillium sp. HSZ-43, along with three known analogues, funicone (2), deoxyfunicone (3) and 3-O-methylfunicone (4). These structures were identified by using spectroscopic methods, including UV, MS, 1D and 2D NMR experiments. The structure of 1 was confirmed by single-crystal X-ray diffraction analysis. All the isolated compounds were evaluated for cytotoxicity against human oral epidermoid carcinoma KB cells, and compound 1 exhibited moderate cytotoxic activity with IC50 value of 4.7 ?M.
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Risk factors for depth of infiltration in the differentiated depressed early gastric carcinoma: a preliminary analysis.
Diagn Pathol
PUBLISHED: 05-26-2014
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To analyze the clinicopathologic factors associated with mucosal and submucosal infiltration in differentiated depressed early gastric cancer, and screening factors that can predict depth of infiltration before endoscopic treatment.
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[Analysis and identification of Poria cocos peels harvested form different producing areas by FTIR and 2D-IR correlation spectroscopy].
Guang Pu Xue Yu Guang Pu Fen Xi
PUBLISHED: 05-15-2014
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Different geographical regions of traditional Chinese medicine (TCM), its chemical composition is different, the accumulation of drug and medicinal properties is different. The accurate identification and analysis of different production area of medicinal herbs is critical for the quality control and pharmacological research of TCM. In this paper, a tri-step infrared spectroscopy (Fourier transform infrared spectroscopy (FTIR) combined with second derivative spectra and two-dimensional correlation infrared spectroscopy (2D-COS) were employed to identify and analyze the main components of Hubei (HB), Anhui (AH), Yun-nan (YN) genuine Poria Cocos peels. The emergence of several characteristic absorption peaks of carbohydrates including 1149, 1079 1036 cm(-1), peaks around 1619, 1315, 780 cm(-1) belonged to calcium oxalate suggested that HB and AH Poria Cocos peels contained calcium oxalate, but peaks around 797, 779, 537, 470 cm(-1) belonged to kaoline suggested that YN Poria Cocos peels contained kaoline. Their carbohydrates were different by comparing the second derivative infrared spectra in the range of 1640-450 cm(-1) and Yongping come from YN contains both calcium oxalate and kaoline. Furthermore, the above differences were visually validated by two-dimensional correlation spectroscopy (2D-COS). It was demonstrated that the Tri-step infrared spectroscopy were successfully applied to fast analyze and identify Poria Cocos peels from different geographical regions and subsequently would be applicable to explain the relevance of geographical regions and medicinal properties for the TCM.
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Functional rescue of Kallmann syndrome-associated prokineticin receptor 2 (PKR2) mutants deficient in trafficking.
J. Biol. Chem.
PUBLISHED: 04-21-2014
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Mutations in the G protein-coupled prokineticin receptor 2 (PKR2) are known to cause Kallmann syndrome and idiopathic hypogonadotropic hypogonadism manifesting with delayed puberty and infertility. Some of the mutant receptors are not routed to the cell surface; instead, they are trapped in the cellular secretory pathway. The cell-permeant agonists/antagonists have been used to rescue some membrane receptors that are not targeted onto the cell membrane. Here, we chose three disease-associated mutations (W178S, G234D, and P290S), which all resulted in retention of PKR2 intracellularly. We show that a small molecule PKR2 antagonist (A457) dramatically increased cell surface expression and rescued the function of P290S PKR2, but had no effect on W178S and G234D PKR2. Furthermore, we also tested chemical chaperone glycerol on the cell surface expression and function of PKR2 mutants. Treatment with 10% glycerol significantly increased the cell surface expression and signaling of P290S and W178S PKR2. These data demonstrate that some Kallmann syndrome-associated, intracellularly retained mutant PKR2 receptors can be functionally rescued, suggesting a potential treatment strategy for patients bearing such mutations.
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Laparoscopic partial nephrectomy for multilocular cystic renal cell carcinoma: a potential gold standard treatment with excellent perioperative outcomes.
World J Surg Oncol
PUBLISHED: 04-07-2014
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To report on the perioperative outcomes of laparoscopic partial nephrectomy (LPN) for multilocular cystic renal cell carcinoma (MCRCC) and evaluate the feasibility of this minimally invasive technique as a potential gold standard treatment for MCRCC.
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Recommendation for a contouring method and atlas of organs at risk in nasopharyngeal carcinoma patients receiving intensity-modulated radiotherapy.
Radiother Oncol
PUBLISHED: 04-07-2014
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To recommend contouring methods and atlas of organs at risk (OARs) for nasopharyngeal carcinoma (NPC) patients receiving intensity-modulated radiotherapy, in order to help reach a consensus on interpretations of OARs delineation.
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CARL lncRNA inhibits anoxia-induced mitochondrial fission and apoptosis in cardiomyocytes by impairing miR-539-dependent PHB2 downregulation.
Nat Commun
PUBLISHED: 03-08-2014
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Abnormal mitochondrial fission participates in the pathogenesis of many diseases. Long non-coding RNAs (lncRNAs) are emerging as new players in gene regulation, but how lncRNAs operate in the regulation of mitochondrial network is unclear. Here we report that a lncRNA, named cardiac apoptosis-related lncRNA (CARL), can suppress mitochondrial fission and apoptosis by targeting miR-539 and PHB2. The results show that PHB2 is able to inhibit mitochondrial fission and apoptosis. miR-539 is responsible for the dysfunction of PHB2 and regulates mitochondrial fission and apoptosis by targeting PHB2. Further, we show that CARL can act as an endogenous miR-539 sponge that regulates PHB2 expression, mitochondrial fission and apoptosis. Our present study reveals a model of mitochondrial fission regulation that is composed of CARL, miR-539 and PHB2. Modulation of their levels may provide a new approach for tackling apoptosis and myocardial infarction.
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Vibrational microspectroscopic identification of powdered traditional medicines: chemical micromorphology of Poria observed by infrared and Raman microspectroscopy.
Spectrochim Acta A Mol Biomol Spectrosc
PUBLISHED: 03-08-2014
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Microscopic identification using optical microscopes is a simple and effective method to identify powdered traditional medicines made from plants, animals and fungi. Sometimes, the criteria based on physical properties of the microscopic characteristics of drug powder may be ambiguous, which makes the microscopic identification method subjective and empirical to some extent. In this research, the vibrational microspectroscopic identification method is proposed for more explicit discrimination of powdered traditional medicines. The chemical micromorphology, i.e., chemical compositions and related physical morphologies, of the drug powder can be profiled objectively and quantitatively by infrared and Raman microspectroscopy, leading to better understanding about the formation mechanisms of microscopic characteristics and more accurate identification criteria. As an example, the powder of Poria, which is one of the most used traditional Chinese medicines, is studied in this research. Three types of hyphae are classified according to their infrared spectral features in the region from 1200 to 900 cm(-1). Different kinds of polysaccharides indicate that these hyphae may be in different stages of the growth. The granular and branched clumps observed by the optical microscope may be formed from the aggregation of the mature hyphae with ?-D-glucan reserves. The newfound spherical particles may originate from the exuded droplets in the fresh Poria because they are both composed of ?-D-glucan. The results are helpful to understand the development of the hyphae and the formation of active polysaccharides in Poria and to establish accurate microspectroscopic identification criteria.
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Preparation of fluorine-doped, carbon-encapsulated hollow Fe3O4 spheres as an efficient anode material for Li-ion batteries.
Nanoscale
PUBLISHED: 03-07-2014
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Herein we report the design and synthesis of fluorine-doped, carbon-encapsulated hollow Fe3O4 spheres (h-Fe3O4@C/F) through mild heating of polyvinylidene fluoride (PVDF)-coated hollow Fe3O4 spheres. The spheres exhibit enhanced cyclic and rate performances. The as-prepared h-Fe3O4@C/F shows significantly improved electrochemical performance, with high reversible capacities of over 930 mA h g(-1) at a rate of 0.1 C after 70 cycles, 800 mA h g(-1) at a rate of 0.5 C after 120 cycles and 620 mA h g(-1) at a rate of 1 C after 200 cycles. This improved lithium storage performance is mainly ascribed to the encapsulation of the spheres with fluorine-doped carbon, which not only improves the reaction kinetics and stability of the solid electrolyte interface film but also prevents aggregation and drastic volume change of the Fe3O4 particles. These spheres thus represent a promising anode material in lithium-ion battery applications.
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Infrared microspectroscopic identification of marker ingredients in the finished herbal products based on the inherent heterogeneity of natural medicines.
Anal Bioanal Chem
PUBLISHED: 03-03-2014
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Finished herbal products (FHPs) are preparations made from one or more herbs. The first stage in assuring the quality, safety, and efficacy of FHPs is to identify the herbs in the products. A new simple and quick method is developed in this research to detect the marker ingredients in FHPs. The inherent chemical heterogeneity of herbs and FHPs makes it possible to resolve different ingredients, without any additional separation or labeling, by infrared microspectroscopic imaging. Therefore, multiple marker ingredients in FHPs can be recognized directly and simultaneously by the infrared microspectroscopic identification method. As an example, all six kinds of herbs in Liuwei Dihuang Wan are identified through the following steps: (1) Each herb is characterized by infrared spectroscopic imaging, then the spectra of the main ingredients are calculated by the combination of principal component analysis, independent component analysis, and alternating least squares. (2) One marker ingredient is chosen for each herb. Ten typical pixels, the spectra of which best match the calculated spectrum of the marker ingredient, are selected by partial least squares target. The average spectrum of the typical pixels is taken as the marker spectrum. (3) Correlation coefficients between the typical pixel spectra and the marker spectrum are calculated. The acceptance correlation threshold is determined through the beta distribution function and then validated by positive and negative samples. (4) Using the above marker spectra and correlation criteria, herbs in the model mixture and the commercial product are identified. Good recognition results reveal the potential of the infrared microspectroscopic identification method in the quality control of herbs and FHPs.
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Site-specific antibody-drug conjugation through glycoengineering.
Bioconjug. Chem.
PUBLISHED: 02-28-2014
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Antibody-drug conjugates (ADCs) have been proven clinically to be more effective anti-cancer agents than native antibodies. However, the classical conjugation chemistries to prepare ADCs by targeting primary amines or hinge disulfides have a number of shortcomings including heterogeneous product profiles and linkage instability. We have developed a novel site-specific conjugation method by targeting the native glycosylation site on antibodies as an approach to address these limitations. The native glycans on Asn-297 of antibodies were enzymatically remodeled in vitro using galactosyl and sialyltransferases to introduce terminal sialic acids. Periodate oxidation of these sialic acids yielded aldehyde groups which were subsequently used to conjugate aminooxy functionalized cytotoxic agents via oxime ligation. The process has been successfully demonstrated with three antibodies including trastuzumab and two cytotoxic agents. Hydrophobic interaction chromatography and LC-MS analyses revealed the incorporation of ~1.6 cytotoxic agents per antibody molecule, approximating the number of sialic acid residues. These glyco-conjugated ADCs exhibited target-dependent antiproliferative activity toward antigen-positive tumor cells and significantly greater antitumor efficacy than naked antibody in a Her2-positive tumor xenograft model. These findings suggest that enzymatic remodeling combined with oxime ligation of the native glycans of antibodies offers an attractive approach to generate ADCs with well-defined product profiles. The site-specific conjugation approach presented here provides a viable alternative to other methods, which involve a need to either re-engineer the antibody sequence or develop a highly controlled chemical process to ensure reproducible drug loading.
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Long non-coding RNAs in stem cells and cancer.
World J Clin Oncol
PUBLISHED: 01-22-2014
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An overwhelming majority of the transcribed genome encodes for non-coding RNA (ncRNA) sequences. Deep sequencing of the transcriptome has uncovered tens of thousands of long ncRNA (lncRNA) sequences. However, little is known regarding the possible functions for a vast majority of these sequences. Among those lncRNAs whose function has been experimentally validated, most serve as regulators of gene expression. LncRNAs have been found to be critical to development and homeostasis and they have been implicated in several pathologies including cancer. Here, we examine the functions and underlying mechanisms of lncRNAs in stem cells and in cancer biology, areas linked by the actions of lncRNAs.
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Selective use of adjuvant chemotherapy for rectal cancer patients with ypN0.
Int J Colorectal Dis
PUBLISHED: 01-15-2014
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The administration of adjuvant chemotherapy for rectal cancer patients with ypN0 is controversial. The purposes of this study were to evaluate the role of adjuvant chemotherapy in ypN0 patients and to optimize its use for these patients.
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Comparison of Long-Term Survival and Toxicity of Cisplatin Delivered Weekly versus Every Three Weeks Concurrently with Intensity-Modulated Radiotherapy in Nasopharyngeal Carcinoma.
PLoS ONE
PUBLISHED: 01-01-2014
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We aimed to compare the long-term survival outcomes and acute toxicity of cisplatin administered weekly versus every three weeks concurrently with intensity-modulated radiotherapy (IMRT) in patients with nasopharyngeal carcinoma (NPC).
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Oxytocin inhibits the activity of acid-sensing ion channels through the vasopressin, V1A receptor in primary sensory neurons.
Br. J. Pharmacol.
PUBLISHED: 01-01-2014
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A growing number of studies have demonstrated that oxytocin (OT) plays an analgesic role in modulation of nociception and pain. Most work to date has focused on the central mechanisms of OT analgesia, but little is known about whether peripheral mechanisms are also involved. Acid-sensing ion channels (ASICs) are distributed in peripheral sensory neurons and participate in nociception. Here, we investigated the effects of OT on the activity of ASICs in dorsal root ganglion (DRG) neurons.
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Signaling role of prokineticin 2 on the estrous cycle of female mice.
PLoS ONE
PUBLISHED: 01-01-2014
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The possible signaling role of prokineticin 2 (PK2) and its receptor, prokineticin receptor 2 (PKR2), on female reproduction was investigated. First, the expression of PKR2 and its co-localization with estrogen receptor (ER?) in the hypothalamus was examined. Sexually dimorphic expression of PKR2 in the preoptic area of the hypothalamus was observed. Compared to the male mice, there was more widespread PKR2 expression in the preoptic area of the hypothalamus in the female mice. The likely co-expression of PKR2 and ER? in the preoptic area of the hypothalamus was observed. The estrous cycles in female PK2-null, and PKR2-null heterozygous mice, as well as in PK2-null and PKR2-null compound heterozygous mice were examined. Loss of one copy of PK2 or PKR2 gene caused elongated and irregular estrous cycle in the female mice. The alterations in the estrous cycle were more pronounced in PK2-null and PKR2-null compound heterozygous mice. Consistent with these observations, administration of a small molecule PK2 receptor antagonist led to temporary blocking of estrous cycle at the proestrous phase in female mice. The administration of PKR2 antagonist was found to blunt the circulating LH levels. Taken together, these studies indicate PK2 signaling is required for the maintenance of normal female estrous cycles.
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[Value of postoperative adjuvant chemotherapy in locally advanced rectal cancer patients with ypT1-4N0 after neo-adjuvant chemoradiotherapy].
Zhonghua Zhong Liu Za Zhi
PUBLISHED: 12-17-2013
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The purpose of this study was to investigate the value of postoperative chemotherapy for locally advanced rectal cancer patients who reached pathological ypT1-4N0 after neo-adjuvant chemoradiotherapy.
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Characterization of a Stem-like Subpopulation in Basal-like Ductal Carcinoma in Situ (DCIS) Lesions.
J. Biol. Chem.
PUBLISHED: 12-02-2013
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Previously, we found that basal-like Ductal Carcinoma In Situ (DCIS) contains cancer stem-like cells. Here, we characterize stem-like subpopulations in a model of basal-like DCIS and identify subpopulations of CD49f+/CD24- stem-like cells which possess ALDH1 activity. We found that these cells show enhanced migration potential compared to non-stem DCIS cells. We also found that the chemopreventive agent sulforaphane (SFN) can target these DCIS stem like cells, reduce Aldehyde Dehydrogenase 1 (ALDH1) expression, and decrease mammosphere and progenitor colony formation. Furthermore, we characterized exosomal trafficking of microRNAs in DCIS and found that several microRNAs (miRs) including miR-140, miR-29a, and miR-21 are differentially expressed in exosomes from DCIS stem-like cells. We found that SFN treatment could reprogram DCIS stem-like cells as evidenced by significant changes in exosomal secretion more closely resembling that of non-stem cancer cells. Finally, we demonstrated that exosomal secretion of miR-140 might impact signaling in nearby breast cancer cells.
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Glycan structure determinants for cation-independent mannose 6-phosphate receptor binding and cellular uptake of a recombinant protein.
Bioconjug. Chem.
PUBLISHED: 11-12-2013
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The cation-independent mannose 6-phosphate receptor (CI-MPR) plays a critical role in intracellular transport of lysosomal enzymes as well as the uptake of recombinant proteins. To define the minimal glycan structure determinants necessary for receptor binding and cellular uptake, we synthesized a series of glycans containing mono-, di-, tri-, tetra-, and hexamannoses terminated with either one or two phosphates for conjugating to a model protein, recombinant human acid ?-glucosidase. A high affinity interaction with the CI-MPR can be achieved for the enzyme conjugated to a dimannose glycan with a single phosphate. However, tightest binding to a CI-MPR affinity column was observed with a hexamannose structure containing two phosphates. Moreover, maximal cellular uptake and a 5-fold improvement in in vivo potency were achieved when the bisphosphorylated hexamannose glycan is conjugated to the protein by a ? linker. Nevertheless, even a monophosphorylated dimannose glycan conjugate showed stronger binding to the receptor affinity column, higher cellular uptake, and significantly greater in vivo efficacy compared to the unconjugated protein which contains a low level of high affinity glycan structure. These results demonstrate that the phosphorylated dimannose moiety appears to be the minimal structure determinant for enhanced CI-MPR binding and that the orientation of the glycan is critical for maximum receptor interaction. In summary, we have improved the understanding of the mechanism of CI-MPR binding and developed a simple alternative for CI-MPR targeting.
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Laparoscopic Versus Open Surgery for Renal Masses with Infrahepatic Tumor Thrombus: The Largest Series of Retroperitoneal Experience from China.
J. Endourol.
PUBLISHED: 11-09-2013
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Abstract Objective: To compare the treatment outcome of laparoscopic versus open surgery for renal masses with infrahepatic (levels I and II) tumor thrombus and analyze the largest series of retroperitoneal experience in such cases from China. Materials and Methods: Forty-nine patients with renal masses with infrahepatic tumor thrombus were analyzed retrospectively between January 2009 and January 2013 at our institution. The diagnosis was confirmed by computed tomography and/or magnetic resonance imaging preoperatively. They were divided into two groups based on the detailed surgical approach applied. Group 1 (A, pure laparoscopic; B, laparoscopic nephrectomy with open thrombectomy) consisted of 17 patients (34.7%) and group 2 (pure open) consisted of 32 patients (65.3%). Results: The two groups were comparable with regard to all of the patients baseline characteristics. All operations were performed successfully without severe complications. In group 1, the mean operative time was 208.5 minutes, the mean estimated blood loss was 147.1?mL, the mean postoperative hospital stay was 5.8 days, and only two patients (11.8%) accepted blood transfusion with a mean quantity of 300?mL. In group 2, the figures were 268.3 minutes, 1345.3?mL, 12.5 days, respectively, and a total of 17 patients (53.1%) received transfusion with a mean quantity of 1488.2?mL. The differences of all these parameters were statistically significant between both groups (p<0.05). However, comparisons between the group 1A and 1B in all of the perioperative characteristics were not statistically significant. During a mean follow-up of 18.2 months, 13 patients in group 1 and 26 patients in group 2 survived. The difference in the cancer-survival rates between both groups was not statistically significant. Conclusions: Although it remains technically complex, demanding and challenging for renal masses with infrahepatic tumor thrombus, laparoscopic surgery with a retroperitoneal approach can make favorable perioperative results and comparable survival rates in selectively less complex patients compared with the traditional open surgery.
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[Urachal carcinoma: experience of a clinical center within 30 years].
Beijing Da Xue Xue Bao
PUBLISHED: 10-19-2013
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To summarize more than thirty years of experience with urachal carcinoma and to discuss the clinical features of urachal carcinoma.
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[Effects of metabolic syndrome on multi-vessel lesions of symptomatic intracranial atherosclerosis].
Zhonghua Yi Xue Za Zhi
PUBLISHED: 10-16-2013
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To explore the effects of metabolic syndrome (MS) on multi-vessel lesions of symptomatic intracranial atherosclerosis.
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[The influence of Co-Cr-Mo alloys on biological behaviour of L929 mouse fibroblasts].
Shanghai Kou Qiang Yi Xue
PUBLISHED: 10-09-2013
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To investigate the influence of dental Co-Cr-Mo alloys on biological behaviour of L929 mouse fibroblasts.
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[Prognostic and long term follow-up analysis of sporadic bilateral renal cell carcinoma].
Beijing Da Xue Xue Bao
PUBLISHED: 08-14-2013
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To investigate the pathological feature, therapy and prognosis of bilateral sporadic renal cell carcinoma.
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[Retrospective analysis of primary renal sarcoma: 17 cases report].
Beijing Da Xue Xue Bao
PUBLISHED: 08-14-2013
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To investigate the clinical characteristics,prognosis, survival and diagnosis of primary renalsarcoma.
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[Surgical management and long-term outcomes of patients with renal cell carcinoma accompanied with venous tumor thrombus].
Beijing Da Xue Xue Bao
PUBLISHED: 08-14-2013
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To evaluate the surgical management and long-term outcomes of patients with renal cell carcinoma and venous tumor thrombus.
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Pattern and risk factors of intravesical recurrence after nephroureterectomy for upper tract urothelial carcinoma: A large Chinese center experience.
J. Formos. Med. Assoc.
PUBLISHED: 08-13-2013
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There is currently no consensus about the pattern and risk factors of bladder recurrence after nephroureterectomy, especially in the Chinese population. We evaluated the pattern and risk factors based on data from a large Chinese center.
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Evaluation of efficacy and safety of sunitinib regimen in 22 patients with metastatic renal cell carcinoma: at least 12-month follow-up.
Chin. Med. J.
PUBLISHED: 08-09-2013
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Sunitinib has been proved an effective new option for treatment of metastatic renal cell carcinoma (mRCC). Analysis of clinical data of 22 patients, who were exposed to sunitinib for at least 1 year, was conducted to evaluate the long-term efficacy and safety of sunitinib for the treatment of mRCC.
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Predictive factors for worse pathological outcomes of upper tract urothelial carcinoma: experience from a nationwide high-volume centre in China.
BJU Int.
PUBLISHED: 07-26-2013
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To analyse the predictive factors for worse pathological outcome (muscle invasive pT2+, non-organ-confined pT3+ or N+ and histological Grade 3) of upper tract urothelial carcinoma (UTUC) in a Chinese population from a nationwide high-volume centre in China.
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A pre-microRNA-149 (miR-149) genetic variation affects miR-149 maturation and its ability to regulate the Puma protein in apoptosis.
J. Biol. Chem.
PUBLISHED: 07-19-2013
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MicroRNAs (miRNAs) are small, single-stranded, noncoding RNAs that function as negative regulators of gene expression. They are transcribed from endogenous DNA and form hairpin structures (termed as pre-miRNAs) that are processed to form mature miRNAs. It remains largely unknown as to the molecular consequences of the natural genetic variation in pre-miRNAs. Here, we report that an A?G polymorphism (rs71428439) is located in Homo sapiens miR-149 stem-loop region. This polymorphism results in a change in the structure of the miR-149 precursor. Our results showed that the genotype distribution of this polymorphism in myocardial infarction cases was significantly different from that in the control subjects. We examined the biological significance of this polymorphism on the production of mature miR-149, and we observed that the G-allelic miR-149 precursor displayed a lower production of mature miR-149 compared with the A-allelic one. Further investigations disclosed that miR-149 could withstand mitochondrial fission and apoptosis through targeting the pro-apoptotic factor p53-up-regulated modulator of apoptosis (Puma). Enforced expression of miR-149 promoted cell survival, whereas knockdown of miR-149 rendered cells to be sensitive to apoptotic stimulation. Intriguingly, the A to G variation led pre-miR-149 to elicit an attenuated effect on the inhibition of mitochondrial fission and apoptosis. Finally, this polymorphism exerts its influence on cardiac function in the mouse model of myocardial infarction. These data suggest that this polymorphism in the miR-149 precursor may result in important phenotypic traits of myocardial infarction. Our findings warrant further investigations on the relationship between miR-149 polymorphism and myocardial infarction.
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[Analysis of Spirulina powder by Fourier transform infrared spectroscopy and calculation of protein content].
Guang Pu Xue Yu Guang Pu Fen Xi
PUBLISHED: 07-12-2013
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Spirulina, Spirulina powder and dextrin standard were analyzed and identified by Infrared (IR) spectroscopy. The main components, protein (1 657 and 1 537 cm(-1)) and carbohydrate (1 069 and 1054 cm(-1)), had distinct fingerprint characteristics of IR spectra. By comparing the IR spectra of Spirulina, Spirulina powder and dextrin standard, the dominant nutrition in Spirulina powder was identified as protein and carbohydrate. The dominant accessory added in Spirulina powder was dextrin. Comparing the IR spectra of Spirulina powder from 28 different factories and figuring out the correlation provides the information about the amount of accessory. A standard curve of the ratio of absorption peak intensities to protein content was constructed to accurately determine the amount of protein in Spirulina powder.
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Sulforaphane inhibits mammary adipogenesis by targeting adipose mesenchymal stem cells.
Breast Cancer Res. Treat.
PUBLISHED: 07-11-2013
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It is now well accepted that tumor cells actively communicate with the tumor microenvironment (e.g., adipocytes) leading to the progression of breast cancer and other malignancies. It is also known that adipose mesenchymal stem cells (MSCs) have the ability to differentiate into mature adipocytes and initiate cytokine signaling within the tumor microenvironment. Here, we examine the role of MSC-differentiated adipocytes on breast cancer cell migration, and test the effects of sulforaphane (SFN, a dietary chemoprevention agent) on adipocyte-breast cancer cell interaction. Our results demonstrate that SFN promotes MSC self-renewal and inhibits adipogenic differentiation. Subsequently, SFN treatment of adipocytes considerably hinders cytokine communication with breast cancer cells, thereby decreasing breast cancer cell migration and tumor formation.
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Prognostic value of chronic hepatitis B virus infection in patients with nasopharyngeal carcinoma: Analysis of 1301 patients from an endemic area in China.
Cancer
PUBLISHED: 06-29-2013
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The current study investigated the prevalence and prognostic value of chronic hepatitis B virus (HBV) infection in patients with nasopharyngeal carcinoma (NPC) from an area in southern China in which HBV and NPC are endemic.
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[Proteome analysis of potato drought resistance variety in Ninglang 182 leaves under drough stress].
Yi Chuan
PUBLISHED: 06-05-2013
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It is utmost important to cultivate potato variety with drought resistance to reduce arid loss by research progress on potato drought resistance molecular mechanism. A comparative study with differences in protein group analysis of potato drought resistance variety in Ninglang 182 leaves was carried out by using two-dimensional gel electrophoresis during drought and normal processing conditions.There were 12 differentially expressed protein spots identified by Electro-phoresis and MALDI-TOF-TOF/MS analysis. The function classification of these proteins results that the potato varieties to drought tolerance could be improved through the protection of photosynthesis and mitochondria, regulation of the signal transduction induced under environmental stress and regulation of plant tissue N and C transport system, these proteins expression were increased under drought.The results showed that these proteins are the drought resistance associated proteins of potato variety in Ninglang 182.This study provided a theoretical basis of the molecular mechanism of improving drought tolerance in order to expound the potato drought resistance variety through multiple paths and the level regulation.
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[Discrimination of eleven genera of Chinese herbs in geraniaceae by FTIR spectroscopy and clustering analysis].
Guang Pu Xue Yu Guang Pu Fen Xi
PUBLISHED: 05-24-2013
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A fast identification method of eleven genera of Chinese herbs in geraniaceae was developed by the combination of Fourier transform infrared spectroscopy with clustering analysis. FTIR spectroscopy was employed to identify and analyze eleven genera of Chinese herbs in geraniaceae. On the basis of a principal component analysis (PCA) model, three genera of Chinese herbs were rapidly classified by using the method of SIMCA clustering analysis. These samples could be successfully classified by SIMCA. Recognition rate and rejection rate reached up to 98%. The accuracy of clustering reached up to 91% during blind sample testing. It is concluded that in combination with clustering analysis, FTIR method provides an effective way to rapidly evaluate Chinese herbs in Geraniaceae.
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Direct observation of bulk and surface chemical morphologies of Ginkgo biloba leaves by Fourier transform mid- and near-infrared microspectroscopic imaging.
Anal Bioanal Chem
PUBLISHED: 05-08-2013
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Fourier transform infrared microspectroscopy is a powerful tool to obtain knowledge about the spatial and/or temporal distributions of the chemical compositions of plants for better understanding of their biological properties. However, the chemical morphologies of plant leaves in the plane of the blade are barely studied, because sections in this plane for mid-infrared transmission measurements are difficult to obtain. Besides, native compositions may be changed by chemical reagents used when plant samples are microtomed. To improve methods for direct infrared microspectroscopic imaging of plant leaves in the plane of the blade, the bulk and surface chemical morphologies of nonmicrotomed Ginkgo biloba leaves were characterized by near-infrared transmission and mid-infrared attenuated total reflection microspectroscopic imaging. A new self-modeling curve resolution procedure was proposed to extract the spectral and concentration information of pure compounds. Primary and secondary metabolites of secretory cavities, veins, and mesophylls of Ginkgo biloba leaf blades were analyzed, and the distributions of cuticle, protein, calcium oxalate, cellulose, and ginkgolic acids on the adaxial surface were determined. By the integration of multiple infrared microspectroscopic imaging and chemometrics methods, it is possible to analyze nonmicrotomed leaves and other plant samples directly to understand their native chemical morphologies in detail.
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Rapid discrimination of Herba Cistanches by multi-step infrared macro-fingerprinting combined with soft independent modeling of class analogy (SIMCA).
Spectrochim Acta A Mol Biomol Spectrosc
PUBLISHED: 05-07-2013
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Herba Cistanche, an important Chinese herbal medicine, has common four species, Cistanche deserticola (CD), Cistanche tubulosa (CT), Cistanche salsa (CS) and Cistanche sinensis (CSN) which have been frequent mixed used. To clarify the sources of Herba Cistanches and ensure the clinical efficacy and safety, a method combing IR macro-fingerprinting with statistical pattern recognition was developed to analyze and discriminate the four species of Herba Cistanche. By comparing FT-IR, second derivative spectral fingerprints via group-peak matching, the similarity to CD and total saccharides (TS) followed an increasing sequence, CTCSN>CS>CD. Characteristic fingerprints of their 2D-IR correlation spectra in 1750-1000 cm(-1) have confirmed the above findings in a more intuitive way. In terms of sources for phenylethanoid glycosides (PhGs), CT can be an ideal alternative species. However, in terms of using them as a whole, more pharmacological study should be conducted due to the different ratios of their chemical constituents, which is also applicable to CSN and CS. Moreover, the four species (179 samples) has been objectively classified by SIMCA based on IR macro-fingerprints.
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Repeated exposure of mouse dermal fibroblasts at a sub-cytotoxic dose of UVB leads to premature senescence: A robust model of cellular photoaging.
J. Dermatol. Sci.
PUBLISHED: 05-06-2013
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Photoaging skin is due to accumulative effect of UV irradiation that mainly imposes its damage on dermal fibroblasts. To mimic the specific cellular responses invoked by long term effect of UVB, it is preferable to develop a photo-damaged model in vitro based on repeated UVB exposure instead of a single exposure.
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Real-time 3-dimensional contrast-enhanced ultrasound in detecting hemorrhage of blunt renal trauma.
Am J Emerg Med
PUBLISHED: 03-25-2013
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The objective of this study is to evaluate the diagnostic value of real-time 3-dimensional contrast-enhanced ultrasound in the hemorrhage of blunt renal trauma.
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Fabrication of a bowl-shaped silver cavity substrate for SERS-based immunoassay.
Analyst
PUBLISHED: 03-12-2013
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In this study, a metal sandwich substrate bridged by an immunocomplex has been created for a surface enhanced Raman scattering (SERS)-based immunoassay. The bottom bowl-shaped silver cavity thin film layer was prepared by electrodeposition using a closely packed monolayer of 700 nm diameter polystyrene spheres as a template. The reflection spectra of the films were recorded as a function of film thickness, and then correlated with SERS enhancement using p-aminothiophenol as the probe molecule. The results demonstrate that SERS enhancement can be maximized when both the frequency of the incident laser and Raman scattering approach the resonance frequency of the localized surface plasmon resonance, providing a guideline for the fabrication and further application of these nanocavity arrays. The second layer of silver was introduced by the interactions between the immunocomplexes in the middle layer of the sandwich architecture and the silver nanoparticles. The proposed structure was used to perform the SERS-based immunoassay. The labeled protein can be detected over a wide concentration range and the detection limit of TRITC and Atto610 labeled proteins were 50 and 5 pg mL(-1), respectively. The results demonstrate that the new SERS substrate is suitable for the quantitative identification of biomolecules.
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Rifaximin versus Nonabsorbable Disaccharides for the Treatment of Hepatic Encephalopathy: A Meta-Analysis.
Gastroenterol Res Pract
PUBLISHED: 03-07-2013
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Background. Many studies have found that the antibiotic rifaximin is effective for the treatment of hepatic encephalopathy. However, there is no uniform view on the efficacy and safety of rifaximin. Methods. We performed a meta-analysis through electronic searches to evaluate the efficacy and safety of rifaximin in comparison with nonabsorbable disaccharides. Results. A total of 8 randomized controlled trials including 407 patients were included. The efficacy of rifaximin was equivalent to nonabsorbable disaccharides according to the statistical data (risk ratio (RR): 1.06, 95% CI: 0.94-1.19; P = 0.34). Analysis showed that patients treated with rifaximin had better results in serum ammonia levels (weighted mean difference (WMD): -10.63, 95% CI: -30.63-9.38; P = 0.30), mental status (WMD: -0.32, 95% CI: -0.67-0.03; P = 0.07), asterixis (WMD: -0.12, 95% CI: -0.31-0.08; P = 0.23), electroencephalogram response (WMD: -0.21, 95% CI: -0.34--0.09; P = 0.0007), and grades of portosystemic encephalopathy (WMD: -2.30, 95% CI: -2.78--1.82; P < 0.00001), but only the last ones had statistical significance. The safety of rifaximin was better than nonabsorbable disaccharides (RR: 0.19, 95% CI: 0.10-0.34; P < 0.00001). Conclusion. Rifaximin is at least as effective as nonabsorbable disaccharides, maybe better for the treatment of hepatic encephalopathy. And the safety of rifaximin is better.
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Prophylactic intravesical chemotherapy to prevent bladder tumors after nephroureterectomy for primary upper urinary tract urothelial carcinomas: a systematic review and meta-analysis.
Urol. Int.
PUBLISHED: 02-21-2013
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Intravesical recurrence after treatment of primary upper urinary tract urothelial carcinomas (UUT-UCs) is common. While intravesical instillation is widely used to prevent recurrence after transurethral resection for primary bladder tumors, there is still no consensus on the prophylactic capability of intravesical chemotherapy in preventing bladder tumor recurrence after surgery for UUT-UCs.
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Clinical analysis of the PADUA and the RENAL scoring systems for renal neoplasms: A retrospective study of 245 patients undergoing laparoscopic partial nephrectomy.
Int. J. Urol.
PUBLISHED: 02-03-2013
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To investigate the clinical significance of preoperative aspects and dimensions used for anatomic (PADUA) and radius exophytic/endophytic nearness anterior/posterior location (RENAL) scoring systems for renal neoplasms in patients undergoing laparoscopic partial nephrectomy.
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A PK2/Bv8/PROK2 antagonist suppresses tumorigenic processes by inhibiting angiogenesis in glioma and blocking myeloid cell infiltration in pancreatic cancer.
PLoS ONE
PUBLISHED: 01-23-2013
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Infiltration of myeloid cells in the tumor microenvironment is often associated with enhanced angiogenesis and tumor progression, resulting in poor prognosis in many types of cancer. The polypeptide chemokine PK2 (Bv8, PROK2) has been shown to regulate myeloid cell mobilization from the bone marrow, leading to activation of the angiogenic process, as well as accumulation of macrophages and neutrophils in the tumor site. Neutralizing antibodies against PK2 were shown to display potent anti-tumor efficacy, illustrating the potential of PK2-antagonists as therapeutic agents for the treatment of cancer. In this study we demonstrate the anti-tumor activity of a small molecule PK2 antagonist, PKRA7, in the context of glioblastoma and pancreatic cancer xenograft tumor models. For the highly vascularized glioblastoma, PKRA7 was associated with decreased blood vessel density and increased necrotic areas in the tumor mass. Consistent with the anti-angiogenic activity of PKRA7 in vivo, this compound effectively reduced PK2-induced microvascular endothelial cell branching in vitro. For the poorly vascularized pancreatic cancer, the primary anti-tumor effect of PKRA7 appears to be mediated by the blockage of myeloid cell migration/infiltration. At the molecular level, PKRA7 inhibits PK2-induced expression of certain pro-migratory chemokines and chemokine receptors in macrophages. Combining PKRA7 treatment with standard chemotherapeutic agents resulted in enhanced effects in xenograft models for both types of tumor. Taken together, our results indicate that the anti-tumor activity of PKRA7 can be mediated by two distinct mechanisms that are relevant to the pathological features of the specific type of cancer. This small molecule PK2 antagonist holds the promise to be further developed as an effective agent for combinational cancer therapy.
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Radiation-induced temporal lobe injury after intensity modulated radiotherapy in nasopharyngeal carcinoma patients: a dose-volume-outcome analysis.
BMC Cancer
PUBLISHED: 01-21-2013
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To identify the radiation volume effect and significant dosimetric parameters for temporal lobe injury (TLI) and determine the radiation dose tolerance of the temporal lobe (TL) in nasopharyngeal carcinoma (NPC) patients treated with intensity modulated radiation therapy (IMRT).
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Distribution and sources of organochlorine pesticides in agricultural soils from central China.
Ecotoxicol. Environ. Saf.
PUBLISHED: 01-14-2013
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There is little information on the organochlorine pesticides (OCPs) residues in agricultural soils of Wuhan, the largest city in central China. Surface soil samples were collected from agricultural soils in Wuhan and analyzed to determine twenty-one OCPs. According to the measured concentrations and detection frequencies, dichlorodiphenyltrichloroethanes (DDTs), hexachlorocyclohexanes (HCHs), heptachlor (HEPT), hexachlorobenzene (HCB) and aldrin were the predominant compounds in soil. DDTs accounted for 77.10% of total OCPs, followed by HCHs (7.83%), aldrin (4.21%), HEPTs (2.82%) and HCB (1.53%). The total DDT concentrations ranged from nd to 1198.0ngg(-1) and the main contaminated areas were distributed in Hannan and Xinzhou districts of Wuhan. The total HCH concentrations ranged from nd to 100.58ngg(-1) in soil and relatively higher levels were observed in soil samples from Huangpi and Hannan districts. Source analysis showed that OCPs residues except heptachlor originated mainly from historical application, besides slight recent introduction at some sites. Based on the China National Soil Quality Standard, DDT pollution in most samples of Wuhan agricultural soils could be considered as no and low contamination, while the level of HCHs was classified as no pollution. Our study indicated that there existed potential exposure risk of OCPs in Wuhan agricultural soils although the use of OCPs has been banned.
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Hydrogen peroxide biosensor based on microperoxidase-11 immobilized in a silica cavity array electrode.
Talanta
PUBLISHED: 01-13-2013
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Hydrogen peroxide biosensor based on the silica cavity array modified indium-doped tin oxide (ITO) electrode was constructed. An array of silica microcavities was fabricated by electrodeposition using the assembled polystyrene particles as template. Due to the resistance gradient of the silica cavity structure, the silica cavity exhibits a confinement effect on the electrochemical reactions, making the electrode function as an array of "soft" microelectrodes. The covalently immobilized microperoxidase-11(MP-11) inside these SiO2 cavities can keep its physiological activities, the electron transfer between the MP-11 and electrode was investigated through electrochemical method. The cyclic voltammetric curve shows a quasi-reversible electrochemical redox behavior with a pair of well-defined redox peaks, the cathodic and anodic peaks are located at -0.26 and -0.15V. Furthermore, the modified electrode exhibits high electrocatalytic activity toward the reduction of hydrogen peroxide and also shows good analytical performance for the amperometric detection of H2O2 with a linear range from 2×10(-6) to 6×10(-4)M. The good reproducibility and long-term stability of this novel electrode not only offer an opportunity for the detection of H2O2 in low concentration, but also provide a platform to construct various biosensors based on many other enzymes.
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Identification of ochratoxin a producing fungi associated with fresh and dry liquorice.
PLoS ONE
PUBLISHED: 01-01-2013
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The presence of fungi on liquorice could contaminate the crop and result in elevated levels of mycotoxin. In this study, the mycobiota associated with fresh and dry liquorice was investigated in 3 producing regions of China. Potential toxigenic fungi were tested for ochratoxin A (OTA) and aflatoxin B1 (AFB1) production using liquid chromatography/mass spectrometry/mass spectrometry. Based on a polyphasic approach using morphological characters, ?-tubulin and RNA polymerase II second largest subunit gene phylogeny, a total of 9 genera consisting of 22 fungal species were identified, including two new Penicillium species (Penicillium glycyrrhizacola sp. nov. and Penicillium xingjiangense sp. nov.). The similarity of fungal communities associated with fresh and dry liquorice was low. Nineteen species belonging to 8 genera were detected from fresh liquorice with populations affiliated with P. glycyrrhizacola, P. chrysogenum and Aspergillus insuetus comprising the majority (78.74%, 33.33% and 47.06% of total) of the community from Gansu, Ningxia and Xinjiang samples, respectively. In contrast, ten species belonging to 4 genera were detected from dry liquorice with populations affiliated with P. chrysogenum, P. crustosum and Aspergillus terreus comprising the majority (64.00%, 52.38% and 90.91% of total) of the community from Gansu, Ningxia and Xinjiang samples, respectively. Subsequent LC/MS/MS analysis indicated that 5 fungal species were able to synthesize OTA in vitro including P. chrysogenum, P. glycyrrhizacola, P. polonicum, Aspergillus ochraceus and A. westerdijkiae, the OTA concentration varied from 12.99 to 39.03 µg/kg. AFB1 was absent in all tested strains. These results demonstrate the presence of OTA producing fungi on fresh liquorice and suggest that these fungi could survive on dry liquorice after traditional sun drying. Penicillium chrysogenum derived from surrounding environments is likely to be a stable contributor to high OTA level in liquorice. The harvesting and processing procedure needs to be monitored in order to keep liquorice free of toxigenic fungi.
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Radiation-induced temporal lobe injury for nasopharyngeal carcinoma: a comparison of intensity-modulated radiotherapy and conventional two-dimensional radiotherapy.
PLoS ONE
PUBLISHED: 01-01-2013
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To compare the radiation-induced temporal lobe injury (TLI) in patients with nasopharyngeal carcinoma (NPC) treated with intensity-modulated radiotherapy (IMRT) or two-dimensional conventional radiotherapy (2D-CRT).
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A bispecific protein capable of engaging CTLA-4 and MHCII protects non-obese diabetic mice from autoimmune diabetes.
PLoS ONE
PUBLISHED: 01-01-2013
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Crosslinking ligand-engaged cytotoxic T lymphocyte antigen-4 (CTLA-4) to the T cell receptor (TCR) with a bispecific fusion protein (BsB) comprised of a mutant mouse CD80 and lymphocyte activation antigen-3 (LAG-3) has been shown to attenuate TCR signaling and to direct T-cell differentiation toward Foxp3(+) regulatory T cells (Tregs) in an allogenic mixed lymphocyte reaction (MLR). Here, we show that antigen-specific Tregs can also be induced in an antigen-specific setting in vitro. Treatment of non-obese diabetic (NOD) female mice between 9-12 weeks of age with a short course of BsB elicited a transient increase of Tregs in the blood and moderately delayed the onset of autoimmune type 1 diabetes (T1D). However, a longer course of treatment (10 weeks) of 4-13 weeks-old female NOD animals with BsB significantly delayed the onset of disease or protected animals from developing diabetes, with only 13% of treated animals developing diabetes by 35 weeks of age compared to 80% of the animals in the control group. Histopathological analysis of the pancreata of the BsB-treated mice that remained non-diabetic revealed the preservation of insulin-producing ?-cells despite the presence of different degrees of insulitis. Thus, a bifunctional protein capable of engaging CTLA-4 and MHCII and indirectly co-ligating CTLA-4 to the TCR protected NOD mice from developing T1D.
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[Clinical study of diethylstilbestrol in hormone refractory prostate cancer].
Zhonghua Yi Xue Za Zhi
PUBLISHED: 11-19-2011
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To summarize the clinical outcome and adverse events of estrogen therapy for hormone refractory prostate cancer.
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What is Visualize?

JoVE Visualize is a tool created to match the last 5 years of PubMed publications to methods in JoVE's video library.

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We use abstracts found on PubMed and match them to JoVE videos to create a list of 10 to 30 related methods videos.

Video X seems to be unrelated to Abstract Y...

In developing our video relationships, we compare around 5 million PubMed articles to our library of over 4,500 methods videos. In some cases the language used in the PubMed abstracts makes matching that content to a JoVE video difficult. In other cases, there happens not to be any content in our video library that is relevant to the topic of a given abstract. In these cases, our algorithms are trying their best to display videos with relevant content, which can sometimes result in matched videos with only a slight relation.