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Find video protocols related to scientific articles indexed in Pubmed.
One-Step Click Engineering Considerably Ameliorates the Practicality of an Unqualified Rhodamine Probe.
ACS Appl Mater Interfaces
PUBLISHED: 11-08-2014
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This study describes the exploitation of click chemistry in the one-step molecular engineering of an unqualified rhodamine probe, leading to its considerable functional enhancement in terms of water solubility, ion selectivity, and usefulness in detecting biological and environmental samples. A dipropargyl rhodamine dye previously identified as an unselective and poorly water-soluble mercury(II) probe was used to couple with an azido polyethylene glycol (PEG) by the Cu(I)-catalyzed azide-alkyne 1,3-dipolar cycloaddition click reaction in almost quantitative yield. The simple click-engineered rhodamine probe shows, remarkably, better water solubility and mercury(II) selectivity comparing to the raw counterpart, and can be used to sensitively image mercury ions internalized by live cells and to accurately quantify the ion spiked in river water specimens. This study provides insights into the simple functional improvement of unqualified molecular dye probes via the efficient "click engineering".
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Preparation of two-qubit steady entanglement through driving a single qubit.
Opt Lett
PUBLISHED: 11-01-2014
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Inspired by a recent paper [J. Phys. B47, 055502 (2014)10.1088/0953-4075/47/5/055502JPAPEH0953-4075], we propose a simplified scheme to generate and stabilize a Bell state of two qubits coupled to a resonator. In the scheme only one qubit is needed to be driven by external classical fields, and the entanglement dynamics is independent of the phases of these fields and insensitive to their amplitude fluctuations. This is a distinct advantage as compared with the previous ones that require each qubit to be addressed by well-controlled classical fields. Numerical simulation shows that the steady singlet state with high fidelity can be obtained with currently available techniques in circuit quantum electrodynamics.
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[Determination of arsenic speciation in scomberomorus niphonius by capillary electrophoresis-inductively coupled plasma mass spectrometry].
Guang Pu Xue Yu Guang Pu Fen Xi
PUBLISHED: 11-01-2014
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A method for the detection of arsenocholine (AsC), arsenobetaine (AsB), As(III), dimethylarsinic (DMA), monomethylarsonic (MMA) and As (V) by capillary electrophoresis-inductively coupled plasma mass spectrometry (CE-ICP-MS) was established. The results showed that the six species of arsenic were separated within 20 min under the optimized conditions. Good linearities of 6 arsenic species were observed in the range from 2 to 50 ?g x L(-1) with the linear correlation greater than 0.996, the detection limits were 0.10-1.08 ?g x L(-1) and the RSDs (n = 5) of the peak areas were smaller than 7%. The method was successfully adopted to the determination of the species in scomberomorus niphonius. The recoveries were between 93% and 98%, and we found the arsenobetaine (AsB) was the main species in the sample. The method was suitable for the analysis of other biological samples with the advantages of good stability, less sample consumption, short analysis time and convenience.
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[Clinicopathologic features and prognosis of T lymphoblastic lymphoma associated with Langerhans cell histiocytosis].
Zhonghua Bing Li Xue Za Zhi
PUBLISHED: 10-28-2014
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To study the clinicopathologic features, immunophenotype and molecular genetic changes of T lymphoblastic lymphoma (T-LBL) associated with Langerhans cell histiocytosis (LCH).
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Mechanisms and energetics of potassium channel block by local anesthetics and antifungal agents.
Biochemistry
PUBLISHED: 10-21-2014
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Many drug molecules inhibit the conduction of several families of cation channels by binding to a small cavity just below the selectivity filter of the channel protein. The exact mechanisms governing drug-channel binding and the subsequent inhibition of conduction are not well understood. Here the inhibition of two K(+) channel isoforms, Kv1.2 and KCa3.1, by two drug molecules, lidocaine and TRAM-34, is examined in atomic detail using molecular dynamics simulations. A conserved valine-alanine-valine motif in the inner cavity is found to be crucial for drug binding in both channels, consistent with previous studies of similar systems. Potential of mean force calculations show that lidocaine in its charged form creates an energy barrier of ?6 kT for a permeating K(+) ion when the ion is crossing over the drug, while the neutral form of lidocaine has no significant effect on the energetics of ion permeation. On the other hand, TRAM-34 in the neutral form is able to create a large energy barrier of ?10 kT by causing the permeating ion to dehydrate. Our results suggest that TRAM-34 analogues that remain neutral and permeable to membranes under acidic conditions common to inflammation may act as possible drug scaffolds for combating local anesthetic failure in inflammation.
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Fluorogenic supramolecular complexes formed between pyrenyl-?-cyclodextrin and glyco-rhodamine for the selective detection of lectins.
Chem. Commun. (Camb.)
PUBLISHED: 10-04-2014
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Fluorogenic supramolecular complexes formed between tubular-shaped pyrenyl-?-cyclodextrins and glyco-rhodamine are determined to respond to a selective lectin with 'turn-on' fluorescence with excellent selectivity over a range of competing species.
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[Effect of electroacupuncture stimulation of back-shu points on expression of TNF-alpha and lipid peroxidation reaction in the liver tissue in non-alcoholic fatty liver disease rats].
Zhen Ci Yan Jiu
PUBLISHED: 09-16-2014
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To observe the effect of electroacupuncture (EA) stimulation (EAS) of back-shu acupoints on expression of tumor necrosis factor-alpha (TNF-alpha) and lipid peroxidase reaction in the liver in non-alcoholic fatty liver disease (NAFLD) rats.
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Characterization and site-directed mutation of a novel aldo-keto reductase from Lodderomyces elongisporus NRRL YB-4239 with high production rate of ethyl (R)-4-chloro-3-hydroxybutanoate.
J. Ind. Microbiol. Biotechnol.
PUBLISHED: 09-05-2014
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A novel aldo-keto reductase (LEK) from Lodderomyces elongisporus NRRL YB-4239 (ATCC 11503) was discovered by genome database mining for carbonyl reduction. LEK was overexpressed in Escherichia coli BL21 (DE3), purified to homogeneity and the catalytic properties were studied. Among the substrates, ethyl 4-chloro-3-oxobutanoate was converted to ethyl (R)-4-chloro-3- hydroxybutanoate ((R)-CHBE), an important pharmaceutical intermediate, with an excellent enantiomeric excess (e.e.) (>99 %). The mutants W28A and S209G obtained by site-directed mutation were identified with much higher molar conversion yields and lower Km values. Further, the constructed coenzyme regeneration system with glucose as co-substrate resulted in a yield of 100 %, an enantioselectivity of >99 %, and the calculated production rate of 56.51 mmol/L/H. These results indicated the potential of LEK for the industrial production of (R)-CHBE and other valuable chiral alcohols.
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Study of structural, electronic and optical properties of tungsten doped bismuth oxychloride by DFT calculations.
Phys Chem Chem Phys
PUBLISHED: 09-02-2014
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First-principle calculations have been carried out to investigate structural stabilities, electronic structures and optical properties of tungsten doped bismuth oxychloride (BiOCl). The structures of substitutional and interstitial tungsten, and in the form of WO6-ligand-doped BiOCl are examined. The substitutional and interstitial tungsten doping leads to discrete midgap states within the forbidden band gap, which has an adverse effect on the photocatalytic properties. On the other hand, the WO6-ligand-doped BiOCl structure induces a continuum of hybridized states in the forbidden gap, which favors transport of electrons and holes and could result in enhancement of visible light activity. In addition, the band gap of WO6-BiOCl decreases by 0.25 eV with valence band maximum (VBM) shifting upwards compared to that of pure BiOCl. By calculating optical absorption spectra of pure BiOCl and WO6-ligand-doped BiOCl structure, it is found that the absorption peak of the WO6-ligand-doped BiOCl structure has a red shift towards visible light compared with that of pure BiOCl, which agrees well with experimental observations. These results reveal the tungsten doped BiOCl system as a promising material in photocatalytic decomposition of organics and water splitting under sunlight irradiation.
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Spatial localization of the JAG1/Notch1/osteopontin cascade modulates extrahepatic metastasis in hepatocellular carcinoma.
Int. J. Oncol.
PUBLISHED: 08-29-2014
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The model of Notch-driven carcinogenesis and development of hepatocellular carcinoma remains controversial and is based on observations of developmental stage- and dose-dependent Notch activation. In this study, the relevance of the spatial distribution of Notch cascade members to the promotion of hepatocellular carcinoma metastasis was evaluated. The spatial expression patterns of the members of the Jagged1 (JAG1)/Notch1 cascade in HCC were evaluated in a tissue microarray of 112 tumors and 46 peri-tumors. Regulation of JAG1/Notch1 on osteopontin (OPN) was evaluated by RNA interference. Tumor cells with JAG1 expressed on the membrane (JAG1(Mem)) were more likely to undergo extrahepatic metastasis [p<0.001; hazard ratio (HR), 0.166; 95% CI, 0.068-0.402], and JAG1(Mem) was a strong independent prognostic factor for metastasis (HR, 0.467; 95% CI, 0.271-0.806; p=0.006). JAG1(Mem) also showed a strong positive correlation with Notch1(Mem). In addition, tumors with JAG1(Mem) expression had more poorly encapsulated membranes (p=0.014). Furthermore, Notch1(Mem) expression correlated with HCC metastasis and was the strongest predictive factor for metastasis. However, in peri-tumoral tissues, most JAG1 (45/46) and Notch1 (41/46) was localized to the cytoplasm. The expression of OPN, one of the main targets of JAG1/Notch1 signaling and a crucial metastasis-related gene in HCC, correlated significantly with JAG1(Mem) expression. Knockdown of JAG1 expression or Notch1 expression induced the downregulation of OPN in HCC cells. Taken together, protein localization is a critical factor affecting the activity of the Notch cascade in the development of hepatocellular carcinoma. Furthermore, our results suggest that the JAG1/Notch1/OPN cascade represents a potential therapeutic target for hepatocellular carcinoma metastasis.
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[Effects of ryegrass and arbuscular mycorrhiza on activities of antioxidant enzymes, accumulation and chemical forms of cadmium in different varieties of tomato].
Huan Jing Ke Xue
PUBLISHED: 08-28-2014
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Pot experiments were carried out to investigate the effects of ryegrass and arbuscular mycorrhiza on the plant growth, malondialdehyde (MDA), antioxidant enzyme activities of leaf and root, accumulation and chemical forms of cadmium (Cd) in tow varieties of tomato when exposed to Cd (20 mg x kg(-1)). The results showed that dry weights of fruit and plant, and contents of malondialdehyde (MDA) and antioxidant enzyme activities of leaf and root, and concentrations and accumulations of Cd significantly differed between two varieties of tomato. Dry weights of fruit, roots, stem, leaf and plant were increased by single or combined remediation of ryegrass and arbuscular mycorrhiza, while MDA contents and antioxidant enzyme activities of leaf and root reduced. The total extractable Cd, F(E), F(W), F(NaCl), F(HAc), F(HCl), and F(R) in fruit of two varieties of tomato reduced by 19.4% - 52.4%, 31.0% - 75.2%, 19.7% - 59.1%, 3.1% - 48.2%, 20.0% - 65.0%, 40.7% - 100.0% and 15.2% - 50.0%, respectively. Cadmium accumulations in tomato were in the order of leaf > stem > fruit > root. Cadmium concentrations in leaf, stem, root and fruit of both varieties decreased by single or combined remediation of ryegrass and arbuscular mycorrhiza, and Cd accumulations of stem and plant of two varieties also reduced. Cd accumulations in fruit of two varieties decreased by 42.9% and 43.7% in the combined remediation treatments, respectively. Tolerance and resistance of 'LUO BEI QI' on Cd was more than 'De Fu mm-8', and Cd concentrations and Cd accumulations in fruit and plant were in the order of 'LUO BEI QI' < 'De Fu mm-8' in the presence or absence of single or combined remediation of ryegrass and arbuscular mycorrhiza.
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Hepatoma-selective imaging of heavy metal ions using a 'clicked' galactosylrhodamine probe.
Chem. Commun. (Camb.)
PUBLISHED: 08-22-2014
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This study depicts the 'click' construction of a water-soluble galactosyl rhodamine that can selectively probe mercury ions internalized by hepatoma cells over other cancer cells.
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[Influence of different drying methods on the quality of Sparganii rhizoma based on multi-index evaluation].
Zhong Yao Cai
PUBLISHED: 08-06-2014
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To study the influence of different drying methods on the quality of Sparganii Rhizoma and obtain the optimal one.
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Role of serum and induced sputum surfactant protein D in predicting the response to treatment in chronic obstructive pulmonary disease.
Exp Ther Med
PUBLISHED: 07-29-2014
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This study was designed to determine the expression of serum and sputum surfactant protein D (SP-D) in chronic obstructive pulmonary disease (COPD) and its association with treatment response. Sixty-five treatment-naive patients with COPD and 26 normal control subjects were recruited in the study. The concentrations of serum and sputum SP-D were measured, and the associations of SP-D with pulmonary function and the modified Medical Research Council dyspnea scale (mMRC) and the St. George's Respiratory Questionnaire (SGRQ) scores before and after three months of treatment with an inhaled corticosteroid and a long-acting ?2-agonist were analyzed. The concentrations of serum and sputum SP-D in the COPD group (45.46±37.78 and 173.23±186.93 ng/ml, respectively) were significantly higher than those of the normal control group (31.68±12.04 and 89.59±70.29 ng/ml, respectively). After three months of treatment, serum SP-D levels were reduced to 30.7±13.9 ng/ml and were significantly lower than the baseline levels (t=2.217, P=0.031). However, no significant reduction in sputum SP-D levels was observed following the treatment (P>0.05). A significant association between baseline sputum SP-D and change in SGRQ activity scores (r=-0.652, P=0.012) was observed; however no association was established with the changes in other clinical profiles following the treatment (P>0.05). This result suggested that an increased baseline sputum SP-D may be a weak predictive indicator of response to treatment with inhaled corticosteroids and long-acting ?2-agonists in patients with COPD.
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Novel multipotent AChEI-CCB attenuates hyperhomocysteinemia-induced memory deficits and Neuropathologies in rats.
J. Alzheimers Dis.
PUBLISHED: 07-16-2014
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Alzheimer's disease (AD) has multiple etiopathogenic factors, yet the definitive cause remains unclear and the therapeutic strategies have been elusive. Combination therapy, as one of the promising treatments, has been studied for years and may exert synergistic beneficial effects on AD through polytherapeutic targets. In this study, we tested the effects of a synthesized juxtaposition (named SCR1693) composed of an acetylcholinesterase inhibitor (AChEI) and a calcium channel blocker (CCB) on the hyperhomocysteinemia (HHcy)-induced AD rat model, and found that SCR1693 remarkably improved the HHcy-induced memory deficits and preserved dendrite morphologies as well as spine density by upregulating synapse-associated proteins PSD95 and synapsin-1. In addition, SCR1693 attenuated HHcy-induced tau hyperphosphorylation at multiple AD-associated sites by regulating the activity of protein phosphatase-2A and glycogen synthase kinase-3?. Furthermore, SCR1693 was more effective than individual administration of both donepezil and nilvadipine which were used as AChEI and CCB, respectively, in the clinical practice. In conclusion, our data suggest that the polytherapeutic targeting juxtaposition SCR1693 (AChEI-CCB) is a promising therapeutic candidate for AD.
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Optofluidics based micro-photocatalytic fuel cell for efficient wastewater treatment and electricity generation.
Lab Chip
PUBLISHED: 07-10-2014
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In this work, an optofluidics based micro-photocatalytic fuel cell with a membrane-free and air-breathing mode was proposed to greatly enhance the cell performance. The incorporation of the optofluidic technology into a photocatalytic fuel cell not only enlarges the specific illumination and reaction area but also enhances the photon and mass transfer, which eventually boosts the photocatalytic reaction rate. Our results show that this new photocatalytic fuel cell yields a much higher performance in converting organics into electricity. A maximum power density of 0.58 mW cm(-2) was achieved. The degradation performance of this new optofluidic micro-photocatalytic fuel cell was also evaluated and the maximum degradation efficiency reached 83.9%. In short, the optofluidic micro-photocatalytic fuel cell developed in this work shows promising potential for simultaneously degrading organic pollutants and generating electricity.
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Selective fluorescence detection of monosaccharides using a material composite formed between graphene oxide and boronate-based receptors.
ACS Appl Mater Interfaces
PUBLISHED: 06-25-2014
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We have developed a novel class of simple materials for sensing monosaccharides by the functionalization of graphene oxide (GO) with boronate-based fluorescence probes (BA1 and BA2). The composite materials were characterized by atomic force microscopy, Raman spectroscopy, and UV-vis/fluorescence spectroscopy. The strong fluorescence of the BA probes is quenched in the presence of GO through fluorescence resonance energy transfer. The BA@GO composite sensors formed provide a useful platform for fluorogenic detection of monosaccharides based on the strong affinity between the boronic acid receptor and monosaccharides. The BA@GO composite sensor displayed a "turn-on" fluorescence response with a good linear relationship toward fructose over a range of other saccharides.
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A genomic search approach to identify carbonyl reductases in Gluconobacter oxydans for enantioselective reduction of ketones.
Biosci. Biotechnol. Biochem.
PUBLISHED: 06-24-2014
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The versatile carbonyl reductases from Gluconobacter oxydans in the enantioselective reduction of ketones to the corresponding alcohols were exploited by genome search approach. All purified enzymes showed activities toward the tested ketoesters with different activities. In the reduction of 4-phenyl-2-butanone with in situ NAD(P)H regeneration system, (S)-alcohol was obtained with an e.e. of up to 100% catalyzed by Gox0644. Under the same experimental condition, all enzymes catalyzed ethyl 4-chloroacetoacetate to give chiral products with an excellent e.e. of up to 99%, except Gox0644. Gox2036 had a strict requirement for NADH as the cofactor and showed excellent enantiospecificity in the synthesis of ethyl (R)-4-chloro-3-hydroxybutanoate. For the reduction of ethyl 2-oxo-4-phenylbutyrate, excellent e.e. (>99%) and high conversion (93.1%) were obtained by Gox0525, whereas the other enzymes showed relatively lower e.e. and conversions. Among them, Gox2036 and Gox0525 showed potentials in the synthesis of chiral alcohols as useful biocatalysts.
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Hybrid polylingual object model: an efficient and seamless integration of Java and native components on the Dalvik virtual machine.
ScientificWorldJournal
PUBLISHED: 06-12-2014
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JNI in the Android platform is often observed with low efficiency and high coding complexity. Although many researchers have investigated the JNI mechanism, few of them solve the efficiency and the complexity problems of JNI in the Android platform simultaneously. In this paper, a hybrid polylingual object (HPO) model is proposed to allow a CAR object being accessed as a Java object and as vice in the Dalvik virtual machine. It is an acceptable substitute for JNI to reuse the CAR-compliant components in Android applications in a seamless and efficient way. The metadata injection mechanism is designed to support the automatic mapping and reflection between CAR objects and Java objects. A prototype virtual machine, called HPO-Dalvik, is implemented by extending the Dalvik virtual machine to support the HPO model. Lifespan management, garbage collection, and data type transformation of HPO objects are also handled in the HPO-Dalvik virtual machine automatically. The experimental result shows that the HPO model outweighs the standard JNI in lower overhead on native side, better executing performance with no JNI bridging code being demanded.
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A new method using xenogeneicacellular dermal matrix in the reconstruction of lacrimal drainage.
Br J Ophthalmol
PUBLISHED: 06-11-2014
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To prospectively evaluate the reliability and efficacy of a new treatment for the reconstruction of the lacrimal duct using a new histo-engineered material, xenogeneic (bovine) acellular dermal matrix.
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Content analysis of physical examination templates in electronic health records using SNOMED CT.
Int J Med Inform
PUBLISHED: 06-08-2014
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Most electronic health record (EHR) systems are built on proprietary information models and terminology, which makes achieving semantic interoperability a challenge. Solving interoperability problems requires well-defined standards. In contrast, the need to support clinical work practice requires a local customization of EHR systems. Consequently, contrasting goals may be evident in EHR template design because customization means that local EHR organizations can define their own templates, whereas standardization implies consensus at some level. To explore the complexity of balancing these two goals, this study analyzes the differences and similarities between templates in use today.
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Complex assembly, crystallization and preliminary X-ray crystallographic analysis of the chicken CD8??-BF2*0401 complex.
Acta Crystallogr F Struct Biol Commun
PUBLISHED: 05-30-2014
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In the process of antigen presentation, the MHCI-CD8 complex is important for immune signal transduction by the activation of cytotoxic T cells. Here, the expression, purification, crystallization and X-ray analysis of the complex of the chicken MHC class I molecule BF2*0401 and CD8?? (CD8??-BF2*0401) are reported. This complex was verified by SDS-PAGE analysis of a CD8??-BF2*0401 crystal, which showed three bands corresponding to the molecular weights of BF2*0401, ?2-microglobulin and CD8?, respectively. The crystal of CD8??-BF2*0401 diffracted to 2.8?Å resolution and belonged to space group P21, with unit-cell parameters a = 90.6, b = 90.8, c = 94.9?Å, ? = 98°. The Matthews coefficient and solvent content were calculated to be 2.88?Å(3)?Da(-1) and ?57.3%, respectively.
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Binding modes of two scorpion toxins to the voltage-gated potassium channel kv1.3 revealed from molecular dynamics.
Toxins (Basel)
PUBLISHED: 05-30-2014
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Molecular dynamics (MD) simulations are used to examine the binding modes of two scorpion toxins, margatoxin (MgTx) and hongotoxin (HgTx), to the voltage gated K+ channel, Kv1.3. Using steered MD simulations, we insert either Lys28 or Lys35 of the toxins into the selectivity filter of the channel. The MgTx-Kv1.3 complex is stable when the side chain of Lys35 from the toxin occludes the channel filter, suggesting that Lys35 is the pore-blocking residue for Kv1.3. In this complex, Lys28 of the toxin forms one additional salt bridge with Asp449 just outside the filter of the channel. On the other hand, HgTx forms a stable complex with Kv1.3 when the side chain of Lys28 but not Lys35 protrudes into the filter of the channel. A survey of all the possible favorable binding modes of HgTx-Kv1.3 is carried out by rotating the toxin at 3° intervals around the channel axis while the position of HgTx-Lys28 relative to the filter is maintained. We identify two possible favorable binding modes: HgTx-Arg24 can interact with either Asp433 or Glu420 on the vestibular wall of the channel. The dissociation constants calculated from the two binding modes of HgTx-Kv1.3 differ by approximately 20 fold, suggesting that the two modes are of similar energetics.
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Association of the variants in the PPARG gene and serum lipid levels: a meta-analysis of 74 studies.
J. Cell. Mol. Med.
PUBLISHED: 05-27-2014
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Considerable studies have been carried out to investigate the relationship between the polymorphisms of PPARG (Pro12Ala, C161T and C1431T) and serum lipid levels, but the results were inconclusive. Hence, we conducted a meta-analysis to clarify the association. MEDLINE, EMBASE and the Cochrane Library databases were searched systematically. The subgroup analysis was performed based on ethnicity. Seventy-four studies with 54,953 subjects were included in this meta-analysis. In Pro12Ala, the group with the 'PP' (C/C genotype) genotype group had lower levels of total cholesterol (TC) (mean difference, MD: -0.02, P < 0.00001; I(2)  = 28%), low-density lipoprotein cholesterol (LDL-C) (MD: -0.02, P < 0.00001; I(2)  = 30%) and higher levels of triglyceride (TG) (MD: 0.06, P < 0.00001; I(2)  = 30%) than the combined 'PA+AA' (PA = C/G genotype, AA = G/G genotype) genotype group in Asian population, and the group with the 'PP' genotype had higher levels of TG (MD: 0.07, P < 0.02; I(2)  = 67%) than the combined 'PA+AA' genotype group in non-Asian population. No statistically significant differences in the levels of TC, TG, high-density lipoprotein cholesterol, LDL-C were detected between different genotypes in C161T(Asian or non-Asian) and C1431T(Asian) polymorphisms. This meta-analysis was a renewed and confirmed study to assess the association between PPARG polymorphisms and serum lipid levels in Asian and non-Asian populations. There is a prominent association between Pro12Ala polymorphism and the levels of TC, LDL-C and TG in Asian population. No statistically significant differences in serum lipid levels were detected between different genotypes in C161T and C1431T polymorphisms.
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Identification of a new bis-amino acid glycoside selectively toxic to multiple myeloma cells.
Carbohydr. Res.
PUBLISHED: 05-19-2014
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A bis-triazolyl phenylalaninyl galactoside was synthesized by a two-fold click reaction between an azido phenylalanine and a di-O-propynyl galactoside. By a cytotoxicity assay the compound was determined to be selectively toxic for multiple myeloma (MM) among a series of cancer cell lines with no toxicity to a control cell line. A Western blot analysis suggested that this compound could potentiate the cleavage of poly ADP-ribose polymerase in MM cells, leading to apoptosis.
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Target-specific imaging of transmembrane receptors using quinonyl glycosides functionalized quantum dots.
Anal. Chem.
PUBLISHED: 05-19-2014
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Here, we describe a novel "switch-on" biosensor based on quinonyl glycosides functionalized quantum dots (QDs) for the specific targeting and imaging of transmembrane glycoprotein receptors on the surface of cancer cells. The design of the quinonyl glycosides lies in that the quinone moiety serves as a quencher of QDs and the glycoside moiety as a biospecific ligand for targeting a receptor. We observed that the quenched photoluminescence of the quinone glycosides functionalized QDs could be significantly recovered by a specific lectin that selectively binds to the glycosides clustering the QDs but was not affected by a panel of nonspecific lectins. Moreover, we determined that quinonyl galactoside functionalized QDs could optically image the asialoglycoprotein receptors of a hepatoma cell line in a target-specific manner. This system might provide new insights into the fabrication of photoluminogenic biosensors for the analysis of the universal ligand-receptor recognitions in nature.
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[The effect and mechanism of curcumin derivative B06 on the myocardium from type 2 diabetic rats].
Zhongguo Ying Yong Sheng Li Xue Za Zhi
PUBLISHED: 04-19-2014
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To investigate the protective effect and mechanism of curcumin derivatives B06 on myocardium from type 2 diabetic rats.
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CXCR7 correlates with the differentiation of hepatocellular carcinoma and suppresses HNF4? expression through the ERK pathway.
Oncol. Rep.
PUBLISHED: 04-16-2014
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Hepatocellular carcinoma (HCC) is a malignancy with dysregulated differentiation. However, effective differentiation therapy for HCC is lacking. Previous evidence suggests that CXCR7 is associated with the differentiation of embryonic stem cells. Here, we evaluated the potential role of CXCR7 in the differentiation of HCC. In HCC cell lines, the expression of cancer stem cell-related markers was assessed by flow cytometry and confirmed by western blot and immunofluorescence analyses. Dimethyl sulfoxide, oncostatin M and dexamethasone were used to induce the differentiation of HCC. Immunohistochemical assay was performed on a tissue microarray based on 112 HCC cases that received hepatectomy. Ligand activation, inhibition assays and RNA interference were used to analyze the regulation of hepatocyte nuclear factor 4? (HNF4?) by the CXCR7 pathway. Huh7 and HCCLM3 cell lines were screened for differentiation induction based on biomarkers of hepatic cancer stem cells. CXCR7 was found to be closely associated with the differentiation of HCC, and an inverse expression trend between CXCR7 and HNF4? was found upon induced differentiation. Clinically, high CXCR7 expression was negatively correlated with HNF4? expression in patients with relatively well-differentiated HCC. Moreover, high CXCR7 expression was correlated with poor overall survival and accelerated post-resection metastasis in HCC with a low HNF4? level. Mechanistically, CXCR7 signaling inhibited HNF4? through extracellular regulated protein kinase (ERK) activation, which was inhibited by U0126, an inhibitor of MAPK/ERK kinases 1 and 2. Knockdown of CXCR7 further confirmed that CXCR7 signaling can regulate HNF4? expression. Taken together, our findings indicate that CXCR7 participates in the differentiation of HCC by regulating HNF4?. The CXCR7-ERK-HNF4? cascade represents a new target for the differentiation therapy of HCC.
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Joint detection of ERCC1 , TUBB3 , and TYMS guidance selection of docetaxel, 5-fluorouracil and cisplatin (DDP) individual chemotherapy in advanced gastric cancer patients.
Eur. J. Med. Res.
PUBLISHED: 04-16-2014
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BackgroundTo investigate the guidance selection of docetaxel (D), cisplatin (DDP) (C), and 5-fluorouracil (5-FU) (F) as individual chemotherapy agents via joint detection of ERCC1, TUBB3, and TYMS genes in patients with advanced gastric cancer (AGC).MethodClinical data of 120 patients with AGC who enrolled in our hospital between May 2009 and May 2012 were analyzed. These patients were randomly assigned to experimental and control groups. The mRNA expression of ERCC1, TUBB3, and TYMS was measured by DNA chip technology in the experimental group. Different chemotherapies were administered according to the mRNA expression levels of the three genes, while DCF chemotherapy was directly applied to the control group. Correlation between the three genes¿ mRNA levels, efficiency rate, the median time to progression (MTP), median survival time (MST) and adverse reactions was evaluated.ResultsAs a result, there was a significant correlation between ERCC1 and TUBB3 mRNA expression (P¿=¿0.005), but no obvious correlation between TUBB3 and TYMS or ERCC1 and TYMS. There was also no significant difference in the efficiency rate of chemotherapy (50 % versus 55 %; P¿=¿0.357) and the MTP (10 months versus 7 months; P¿=¿0.091) between the two groups. However, there was obvious significance in MST (13.7 months versus 11.6 months; P¿=¿0.004). Additionally, the experimental group provided us with a more effective way for controlling adverse reactions to chemotherapy.ConclusionCombination regimen of D, C, and F in AGC patients according to their ERCC1, TUBB3, and TYMS mRNA expression level may reduce adverse reactions and improve MST.
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Fluorogenic resveratrol-confined graphene oxide for economic and rapid detection of Alzheimer's disease.
ACS Appl Mater Interfaces
PUBLISHED: 04-08-2014
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Developing an effective means for the real-time probing of amyloid ? (A?) that is closely implicated in Alzheimer's disease (AD) could help better understand and monitor the disease. Here we describe an economic approach based on the simple composition of a natural product, resveratrol (Res), with graphene oxide (GO) for the rapid, fluorogenic recognition of A?. The Res@GO composite has proved specific for A? over a range of proteins and ions, and could sensitively capture both A? monomers and fibers in a physiological buffer solution within only 3 min. The composite can also fluorescently image amyloid deposits in a mouse brain section within 30 min. This new protocol is much cheaper and more timesaving than the conventional immunofluorescence staining technique employed clinically, providing an economic tool for the concise detection of AD.
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Crystallization and preliminary crystallographic studies of the complement 1qA globular domain from zebrafish, Dare-C1qAgD.
Acta Crystallogr F Struct Biol Commun
PUBLISHED: 03-31-2014
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Complement 1q (C1q) is the first component of the complement system which can initiate the classical complement pathway. In human, C1q is composed of 18 polypeptide chains: six C1qA chains, six C1qB chains and six C1qC chains. Each chain has a signal peptide and is comprised of a collagen-like region and a C-terminal C1q globular domain (C1qgD), which is organized as a heterotrimer. C1qgD can recognize antigen-antibody complexes containing IgG and IgM or can bind directly to the C-reactive protein. Although the classical complement pathway is found from fish to mammals, only the human C1qgD structure has been determined. Compared with that of mammals, fish C1q exhibits similar immune functions and genome arrangement. In order to illustrate the structure of C1qgD in fish, zebrafish (Danio rerio) C1qA globular domain (Dare-C1qAgD) was expressed, purified and crystallized. X-ray diffraction data were collected from a crystal to a resolution of 2.05?Å; the crystal belonged to the orthorhombic space group P2?2?2?, with unit-cell parameters a=50.347, b=85.059, c=95.560?Å. It contained three molecules in the asymmetric unit. The Matthews coefficient value VM was 2.31?Å3?Da(-1), with a calculated solvent content of 46.7%. The data will help to give insight into the structural basis of C1qA in fish species.
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Label-free detection of serum proteins using surface-enhanced Raman spectroscopy for colorectal cancer screening.
J Biomed Opt
PUBLISHED: 03-26-2014
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Surface-enhanced Raman scattering (SERS) spectra of serum proteins purified from human serum samples were employed to detect colorectal cancer. Acetic acid as a new aggregating agent was introduced to increase the magnitude of the SERS enhancement. High-quality SERS spectra of serum proteins were acquired from 103 cancer patients and 103 healthy volunteers. Tentative assignments of SERS bands reflect that some specific biomolecular contents and protein secondary structures change with colorectal cancer progression. Principal component analysis combined with linear discriminant analysis was used to assess the capability of this approach for identifying colorectal cancer, yielding diagnostic accuracies of 100% (sensitivity: 100%; specificity: 100%) based on albumin SERS spectroscopy and 99.5% (sensitivity: 100%; specificity: 99%) based on globulin SERS spectroscopy, respectively. A partial least squares (PLS) approach was introduced to develop diagnostic models. An albumin PLS model successfully predicted the unidentified subjects with a diagnostic accuracy of 93.5% (sensitivity: 95.6%; specificity: 91.3%) and the globulin PLS model gave a diagnostic accuracy of 93.5% (sensitivity: 91.3%; specificity: 95.6%). These results suggest that serum protein SERS spectroscopy can be a sensitive and clinically powerful means for colorectal cancer detection.
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Correlation of pulmonary function indexes determined by low-dose MDCT with spirometric pulmonary function tests in patients with chronic obstructive pulmonary disease.
AJR Am J Roentgenol
PUBLISHED: 03-26-2014
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The objective of our study was to evaluate the correlation between pulmonary function indexes determined by low-dose MDCT and those obtained from routine spirometric pulmonary function tests (PFTs) in patients with chronic obstructive pulmonary disease (COPD).
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Crystallization and preliminary X-ray diffraction analysis of a single variable domain of the immunoglobulin superfamily in amphioxus, Amphi-IgSF-V.
Acta Crystallogr F Struct Biol Commun
PUBLISHED: 03-24-2014
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Amphioxus is regarded as an essential animal model for the study of immune evolution. Discovery of new molecules with the immunoglobulin superfamily (IgSF) variable (V) domain in amphioxus would help in studying the evolution of IgSF V molecules in the immune system. A protein was found which just contains only one IgSF V domain in amphioxus, termed Amphi-IgSF-V; it has over 30% sequence identity to the V domains of human immunoglobulins and mammalian T-cell receptors. In order to clarify the three-dimensional structure of this new molecule in amphioxus, Amphi-IgSF-V was expressed, purified and crystallized, and diffraction data were collected to a resolution of 1.95?Å. The crystal belonged to space group P3221, with unit-cell parameters a = b = 53.9, c = 135.5?Å. The Matthews coefficient and solvent content were calculated to be 2.58?Å(3)?Da(-1) and 52.38%, respectively. The results will provide structural information to study the evolution of IgSF V molecules in the immune system.
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A 'clicked' tetrameric hydroxamic acid glycopeptidomimetic antagonizes sugar-lectin interactions on the cellular level.
Sci Rep
PUBLISHED: 02-27-2014
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A tetrameric N-acetyl galactosaminyl (GalNAc) peptidomimetic was constructed by N-acetylation of repeating proline-based hydroxamic acid units, followed by a convergent 'click chemistry' coupling. This novel glycopeptidomimetic was determined to effectively antagonize the interaction between a transmembrane hepatic lectin and GalNAc on the cellular level.
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Substitution pattern reverses the fluorescence response of coumarin glycoligands upon coordination with silver (I).
Sci Rep
PUBLISHED: 02-05-2014
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Development of sugar-based fluorescence (FL) chemo-probes is of much interest since sugars are biocompatible, water-soluble and structurally rigid natural starting materials. We report here that fluorescent glycoligands with two triazolyl coumarin moieties installed onto the different positions of an identical glucosyl nucleus exert completely reversed optical response to a metal ion. C3,4-, C2,3- and C4,6-di-substituted coumarin glucosides synthesized by a click reaction similarly showed a selective FL variation in the presence of silver (I) among a range of metal cations in an aqueous solution. However, the variation was determined to be converse: the FL of the C3,4-ligand was quenched whereas that of the C2,3/C4,6-ligand tangibly enhanced. FL and NMR titrations suggested that this divergence was due to the distinct complexation modes of the conformationally constrained ligands with the ion. The optimal motifs of the ligand-ion complexation were predicted by a computational simulation. Finally, the C2,3-ligand was determined to be of low cytotoxicity and applicable in the FL imaging of silver ions internalized by live cells.
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Sinks as limited resources? A new indicator for evaluating anthropogenic material flows.
Ecol Indic
PUBLISHED: 01-19-2014
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Besides recyclables, the use of materials inevitably yields non-recyclable materials such as emissions and wastes for disposal. These flows must be directed to sinks in a way that no adverse effects arise for humans and the environment. The objective of this paper is to present a new indicator for the assessment of substance flows to sinks on a regional scale. The indicator quantifies the environmentally acceptable mass share of a substance in actual waste and emission flows, ranging from 0% as worst case to 100% as best case. This paper consists of three parts: first, the indicator is defined. Second, a methodology to determine the indicator score is presented, including (i) substance flows analysis and (ii) a distant-to-target approach based on an adaptation of the Ecological Scarcity Method 2006. Third, the metric developed is applied in three case studies including copper (Cu) and lead (Pb) in the city of Vienna, and perfluorooctane sulfonate (PFOS) in Switzerland. The following results were obtained: in Vienna, 99% of Cu flows to geogenic and anthropogenic sinks are acceptable when evaluated by the distant-to-target approach. However, the 0.7% of Cu entering urban soils and the 0.3% entering receiving waters are beyond the acceptable level. In the case of Pb, 92% of all flows into sinks prove to be acceptable, and 8% are disposed of in local landfills with limited capacity. For PFOS, 96% of all flows into sinks are acceptable. 4% cannot be evaluated due to a lack of normative criteria, despite posing a risk for human health and the environment. The examples demonstrate the need (i) for appropriate data of good quality to calculate the sink indicator and (ii) for standards, needed for the assessment of substance flows to urban soils and receiving waters. This study corroborates that the new indicator is well suited as a base for decisions regarding the control of hazardous substances in waste and environmental management.
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Isolation an Aldehyde Dehydrogenase Gene from Metagenomics Based on Semi-nest Touch-Down PCR.
Indian J. Microbiol.
PUBLISHED: 01-16-2014
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Culture-independent approaches to analyze metagenome are practical choices for rapid exploring useful genes. The mg-MSDH gene, acquired from the hot spring metagenomic, was retrieved full lengths of functional gene using semi-nest touch-down PCR. Two pairs of degenerate primers were used to separate seven conserve partial sequences by semi-nest touch-down PCR. One of them showed similarity with aldehyde dehydrogenase was used as a target fragment for isolating full-length sequence. The full-length mg-MSDH sequence contained a 1,473 bp coding sequence encoding a 490-amino-acid polypeptide and assigned an accession number JQ715422 in Genbank. The upstream sequences TAGGAG of the start codon (GTG), suggested that was a ribosome binding site. The coding sequence of mg-MSDH was ligated to pET-303 vector and the reconstructive plasmid was successfully overexpressed in E. coli. The purified recombinant mg-MSDH enzyme showed propionaldehyde oxidative activity of 3.0 U mg(-1) at 37 °C.
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Sex-Determination Gene SRY Potentially Associates with Poor Prognosis but Not Sex Bias in Hepatocellular Carcinoma.
Dig. Dis. Sci.
PUBLISHED: 01-08-2014
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Gender disparity is well known in hepatocellular carcinoma (HCC). SRY is a critical sex-determination gene involved in embryonic development.
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Characterization of Lung Function Impairment in Adults with Bronchiectasis.
PLoS ONE
PUBLISHED: 01-01-2014
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Characteristics of lung function impairment in bronchiectasis is not fully understood.
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Goosecoid promotes the metastasis of hepatocellular carcinoma by modulating the epithelial-mesenchymal transition.
PLoS ONE
PUBLISHED: 01-01-2014
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The homeobox gene, goosecoid (GSC), is a transcription factor that participates in cell migration during embryonic development. Because cell migration during development has characteristics similar to cell invasion during metastasis, we evaluated the potential role of GSC in the metastasis of hepatocellular carcinoma (HCC). GSC expression in HCC cell lines and tissues was evaluated, and its effects on the migration potential of HCC cells were determined by GSC knock-down and overexpression methods. In addition, the prognostic role of GSC expression in the metastasis of cancer cells in HCC patients was determined. Our data showed that GSC was highly expressed in several HCC cell lines, particularly in a highly metastatic HCC cell line. Overexpression of GSC promoted cell migration and invasion of HCC cells in vitro. Gain-of-function induced the epithelial-mesenchymal transition but not collective cell migration, whereas loss-of-function induced the reverse change. High-level expression of GSC correlated closely with poor survival and lung metastasis in HCC patients; lung metastases showed more upregulated GSC expression than the primary tumor. We conclude that GSC promotes metastasis of HCC potentially through initiating the epithelial-mesenchymal transition. GSC is also a prognostic factor for poor survival and metastasis of HCC, which suggests its potential as a therapeutic target for metastatic HCC.
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Partially overlapping sensorimotor networks underlie speech praxis and verbal short-term memory: evidence from apraxia of speech following acute stroke.
Front Hum Neurosci
PUBLISHED: 01-01-2014
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We tested the hypothesis that motor planning and programming of speech articulation and verbal short-term memory (vSTM) depend on partially overlapping networks of neural regions. We evaluated this proposal by testing 76 individuals with acute ischemic stroke for impairment in motor planning of speech articulation (apraxia of speech, AOS) and vSTM in the first day of stroke, before the opportunity for recovery or reorganization of structure-function relationships. We also evaluated areas of both infarct and low blood flow that might have contributed to AOS or impaired vSTM in each person. We found that AOS was associated with tissue dysfunction in motor-related areas (posterior primary motor cortex, pars opercularis; premotor cortex, insula) and sensory-related areas (primary somatosensory cortex, secondary somatosensory cortex, parietal operculum/auditory cortex); while impaired vSTM was associated with primarily motor-related areas (pars opercularis and pars triangularis, premotor cortex, and primary motor cortex). These results are consistent with the hypothesis, also supported by functional imaging data, that both speech praxis and vSTM rely on partially overlapping networks of brain regions.
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Expert consensus on acute exacerbation of chronic obstructive pulmonary disease in the People's Republic of China.
Int J Chron Obstruct Pulmon Dis
PUBLISHED: 01-01-2014
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Chronic obstructive pulmonary disease (COPD) is a common disease that severely threatens human health. Acute exacerbation of COPD (AECOPD) is a major cause of disease progression and death, and causes huge medical expenditures. This consensus statement represents a description of clinical features of AECOPD in the People's Republic of China and a set of recommendations. It is intended to provide clinical guidelines for community physicians, pulmonologists and other health care providers for the prevention, diagnosis, and treatment of AECOPD.
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Dynamic expression patterns of differential proteins during early invasion of hepatocellular carcinoma.
PLoS ONE
PUBLISHED: 01-01-2014
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Tumor cell invasion into the surrounding matrix has been well documented as an early event of metastasis occurrence. However, the dynamic expression patterns of proteins during early invasion of hepatocellular carcinoma (HCC) are largely unknown. Using a three-dimensional HCC invasion culture model established previously, we investigated the dynamic expression patterns of identified proteins during early invasion of HCC.
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Macrolide therapy in adults and children with non-cystic fibrosis bronchiectasis: a systematic review and meta-analysis.
PLoS ONE
PUBLISHED: 01-01-2014
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A systematic review and meta-analysis was conducted to evaluate the efficacy and safety of macrolide therapy in adults and children with bronchiectasis.
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Human Enterovirus 71 Uncoating Captured at Atomic Resolution.
J. Virol.
PUBLISHED: 12-18-2013
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Human enterovirus 71 (EV71) is the major causative agent of severe hand-foot-and-mouth diseases (HFMD) in young children, and structural characterization of EV71 during its lifecycle can aid in the development of therapeutics against HFMD. Here, we present the atomic structures of the full virion and an uncoating intermediate of a clinical EV71 C4 strain to illustrate the structural changes in the full virion that lead to the formation of the uncoating intermediate prepared for RNA release. Although the VP1 N-terminal regions, observed to penetrate through the junction channel at the quasi 3-fold axis in the uncoating intermediate of coxsackievirus A16, were not observed in the EV71 uncoating intermediate, drastic conformational changes occur in this region, as has been observed in all capsid proteins. Additionally, the RNA genome interacts with the N-terminal extensions of VP1 and residues 32-36 of VP3, both of which are situated at the bottom of the junction. These observations highlight the importance of the junction for genome release. Furthermore, EV71 uncoating is associated with apparent rearrangements and expansion around the 2- and 5-fold axes without obvious changes around the 3-fold axes. Therefore, these structures enabled the identification of hot spots for capsid rearrangements, which led to the hypothesis that the protomer interface near the junction and the 2-fold axis permits the opening of large channels for the exit of polypeptides and viral RNA, which is an uncoating mechanism that is likely conserved in enteroviruses.
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[Effects of mechanical stimulation on expression of autoantigens in myoblasts].
Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi
PUBLISHED: 11-28-2013
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To explore the effects of mechanical stimulation on the expression of autoantigens in myoblasts.
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Optofluidic Microreactors with TiO2-Coated Fiberglass.
ACS Appl Mater Interfaces
PUBLISHED: 11-26-2013
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Optofluidic microreactors are promising prospects for photocatalytic reactions. However, because the flow type in conventional designs is typically laminar, the mass transport mainly relies on diffusion, and thus the rate of mass transport is limited. Accordingly, poor mass transport reduces the photocatalytic reaction rate. To alleviate the limitation of mass transport, in this work, we proposed a novel optofluidic microreactor with TiO2-coated fiberglasses immersed in the microreaction chamber. Such a design enables enhanced mass transport by shortening the transport length and inducing the perturbation to liquid flow so as to improve the performance. We demonstrated the feasibility of the optofluidic microreactor with the TiO2-coated fiberglass by the photocatalytic water treatment of methylene blue under UV irradiation. Results showed that the proposed optofluidic microreactor yielded much higher degradation efficiency than did the conventional optofluidic microreactor as a result of enhanced mass transport. The microreactor with the TiO2-coated fiberglass showed a 2-3-fold improvement in the reaction rate constant as opposed to conventional ones. The maximal increment of the degradation efficiency can reach more than 40%.
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Highly selective antibacterial activities of silver nanoparticles against Bacillus subtilis.
J Nanosci Nanotechnol
PUBLISHED: 11-20-2013
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Silver nanoparticles (AgNPs) with different sizes (5, 15 and 55 nm) were synthesized via simple method, and characterized by powder X-ray diffraction (XRD), transmission electron microscopy (TEM), energy-dispersive X-ray microanalysis (EDX) and ultraviolet-visible absorption spectroscopy (UV-Vis). The antibacterial activities of the prepared AgNPs against Gram-negative Escherichia coli (E. coli), Gram-positive Staphylococcus aureus (S. aureus) and Bacillus subtilis (B. subtilis) were evaluated by inhibition zone, inhibition curve, and colony counting methods. The results showed that the AgNPs exhibited obvious bacterium-selective and size-dependent antibacterial activities. The Gram-positive bacteria S. aureus and B. subtilis were more sensitive to AgNPs than Gram-negative bacterium E. coli. Interestingly, AgNPs displayed remarkably antibacterial activities against B. subtilis among Gram-positive bacteria, regardless of whether in separately or cocultured bacteria. It also showed that AgNPs with 5 nm in size presented the highest antibacterial activity against both Gram-negative and Gram-positive bacteria. The effects of AgNPs on the membrane leakage of the reducing sugars from three bacteria were also measured by 3,5-dinitrosalicylic acid method. The leakage amount of reducing sugars from B. subtilis was the highest among the tested bacteria, indicating that AgNPs could damage the structure of bacteria cell membrane and resulted in the leakage of reducing sugars, leading to the death of bacteria.
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Core-shell to yolk-shell nanostructure transformation by a novel sacrificial template-free strategy.
Chem. Commun. (Camb.)
PUBLISHED: 11-02-2013
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Au-conducting polymer core-shell nanostructures have been transformed into yolk-shell nanostructures with enhanced catalytic activity through facile swelling-evaporation processes without any sacrificial template.
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Cloning and expression characterization of the chicken Piwil1 gene.
Mol. Biol. Rep.
PUBLISHED: 10-26-2013
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Piwi gene involves in the germline stem cells self-renewal, transposon silencing and post-transcriptional gene regulation in the majority of organisms; however, the biological function of Piwi gene in poultry remains unclear. Here we cloned the Piwi-like 1 (Piwil1) gene and characterized its expression in the Langshan chickens during the development. The results showed that the PIWIL1 protein was the homolog of mice MIWI and human HIWI proteins (100 % identity), and encoded a cytoplasmic protein including the two conserved domains PAZ and PIWI. In males, the expression of Piwil1 gene showed a bimodal distribution in the gonads during embryogenesis with peaks at embryonic 14.5 and 17.5-18.5 days respectively. After puberty, the expression of Piwil1 gene increased sharply and reached a high level at the sexual maturity. The mRNA expression of Piwil1 gene at 27 weeks of age is 35-40 times that of 0 week of age, indicating that the high expression of Piwil1 gene was essential to maintain the spermatogenesis. In females, the expression of Piwil1 gene showed a unimodal distribution in the embryonic gonads. A strong peak appeared at E16.5-17.5d when the primary oocytes have entered the prophase I of meiosis. Subsequently, the expression of Piwil1 gene decreased gradually and kept at the low level during the embryogenesis. So Piwil1 gene was likely to play an important role during the meiosis I. This report filled in partly the gap of the Piwi gene researches in poultry and defined our research directions in future.
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Twitch mouth pressure and disease severity in patients with chronic obstructive pulmonary disease.
Respir Care
PUBLISHED: 10-15-2013
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Patients with chronic obstructive pulmonary disease (COPD) have impaired respiratory muscle strength. Twitch mouth pressure (TwPM) in response to magnetic stimulation of the cervical nerve has been suggested to clinically reflect inspiratory muscle strength. However, studies on TwPM values and their relationship with disease severity are limited. Thus, we tested the TwPM values of COPD patients and investigated the relationship of these values with disease severity.
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Revisit of a dipropargyl rhodamine probe reveals its alternative ion sensitivity in both a solution and live cells.
Analyst
PUBLISHED: 10-03-2013
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This study reveals that a dipropargyl rhodamine B derivative previously described as a reaction-based irreversible palladium probe responds, however, more sensitively to mercury with a reversible "turn-on" fluorescence. The probe also shows a much better imaging ability for mercury than for palladium in live cells.
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Rapid and nondestructive method for evaluation of embryo culture media using drop coating deposition Raman spectroscopy.
J Biomed Opt
PUBLISHED: 08-28-2013
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ABSTRACT. In this study, a rapid and simple method which combines drop coating deposition and Raman spectroscopy (DCDR) was developed to characterize the dry embryo culture media (ECM) droplet. We demonstrated that Raman spectra obtained from the droplet edge presented useful and characteristic signatures for protein and amino acids assessment. Using a different analytical method, scanning electron microscopy coupled with energy dispersive X-ray analysis, we further confirmed that Na, K, and Cl were mainly detected in the central area of the dry ECM droplet while sulphur, an indicative of the presence of macromolecules such as proteins, was mainly found at the periphery of the droplet. In addition, to reduce sample preparation time, different temperatures for drying the droplets were tested. The results showed that drying temperature at 50°C can effectively reduce the sample preparation time to 6 min (as compared to 50 min for drying at room temperature, ?25°C) without inducing thermal damage to the proteins. This work demonstrated that DCDR has potential for rapid and reliable metabolomic profiling of ECM in clinical applications.
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[Advances in studies on toxicity of aconite].
Zhongguo Zhong Yao Za Zhi
PUBLISHED: 08-16-2013
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Aconite has the efficacy of reviving yang for resuscitation, dispelling cold and relieving pain, which is widely used in clinic, and shows unique efficacy in treating severe diseases. However, aconite has great toxicity, with obvious cardio-toxicity and neurotoxicity. Its toxicological mechanism main shows in the effect on voltage-dependent sodium channels, release of neurotransmitters and changes in receptors, promotion of lipid peroxidation and cell apoptosis in heart, liver and other tissues. Aconite works to reduce toxicity mainly through compatibility and processing. Besides traditional processing methods, many new modern processing techniques could also help achieve the objectives of detoxification and efficacy enhancement. In order to further develop the medicinal value of aconite and reduce its side effect in clinical application, this article gives comprehensive comments on aconites toxicity characteristics, mechanism and detoxification methods on the basis of relevant reports for aconites toxicity and the authors experimental studies.
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Taxifolin prevents diabetic cardiomyopathy in vivo and in vitro by inhibition of oxidative stress and cell apoptosis.
Food Chem. Toxicol.
PUBLISHED: 07-23-2013
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Diabetic cardiomyopathy has been increasingly recognized as an important cause of heart failure in diabetic patients. Excessive oxidative stress has been suggested to play a critical role in the development of diabetic cardiomyopathy. The objective of this study was to investigate the potential protective effects and mechanisms of taxifolin on cardiac function of streptozotocin-induced diabetic mice and on hyperglycemia-induced apoptosis of H9c2 cardiac myoblasts. In vivo study revealed that taxifolin improved diastolic dysfunction, ameliorated myocardium structure abnormality, inhibited myocyte apoptosis and enhanced endogenous antioxidant enzymes activities. Interestingly, taxifolin reduced angiotensin II level in myocardium, inhibited NADPH oxidase activity, and increased JAK/STAT3 activation. In vitro investigation demonstrated that taxifolin inhibited 33mM glucoseinduced H9c2 cells apoptosis by decreasing intracellular ROS level. It also inhibited caspase-3 and caspase-9 activation, restored mitochondrial membrane potential, and regulated the expression of proteins related to the intrinsic pathway of apoptosis, thus inhibiting the release of cytochrome c from mitochondria into the cytoplasm. In conclusion, taxifolin exerted cardioprotective effects against diabetic cardiomyopathy by inhibiting oxidative stress and cardiac myocyte apoptosis and might be a potential agent in the treatment of diabetic cardiomyopathy.
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Global RNA sequencing reveals that genotype-dependent allele-specific expression contributes to differential expression in rice F1 hybrids.
BMC Plant Biol.
PUBLISHED: 06-25-2013
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Extensive studies on heterosis in plants using transcriptome analysis have identified differentially expressed genes (DEGs) in F1 hybrids. However, it is not clear why yield in heterozygotes is superior to that of the homozygous parents or how DEGs are produced. Global allele-specific expression analysis in hybrid rice has the potential to answer these questions.
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Vascular endothelial cells facilitated HCC invasion and metastasis through the Akt and NF-?B pathways induced by paracrine cytokines.
J. Exp. Clin. Cancer Res.
PUBLISHED: 06-24-2013
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It is well documented that cancer cells secrete angiogenic factors to recruit and sustain tumor vascular networks. However, little is known about the effects of endothelial cells on the behavior of tumor cells. The study here was to determine the roles of endothelial cells in HCC cell growth, migration and invasion.
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Spectrum and antimicrobial resistance of common pathogenic bacteria isolated from patients with acute exacerbation of chronic obstructive pulmonary disease in mainland of China.
Chin. Med. J.
PUBLISHED: 06-22-2013
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Bacteria-induced respiratory infection has been long considered to be the major cause of acute exacerbation of chronic obstructive pulmonary disease (AECOPD). Therefore, a clear picture about the distribution and drug-resistance of pathogenic bacteria in the lower airways should be helpful for treatment of the disease. So far, data on this topic among Chinese are lacking.
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T-cell apoptosis induced by intratumoral activated hepatic stellate cells is associated with lung metastasis in hepatocellular carcinoma.
Oncol. Rep.
PUBLISHED: 04-09-2013
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Profound T cell inhibitory activity of hepatic stellate cells (HSCs) in vitro has recently been described in hepatocellular carcinoma (HCC). In the present study, we investigated the immune inhibitory activity of HSCs in vivo in an orthotopic rat HCC model with lung metastasis. Rats (n=24) were randomly sacrificed on days 7, 14, 21 and 28 (n=4 each). Lung tissues were stained with hematoxylin and eosin. Liver sections were stained for immunofluorescence analysis. T-cell apoptosis was detected using double staining for terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL). Staining revealed marked and continuous accumulation of ?-smooth muscle actin with tumor progression after orthotopic tumor implantation in rat liver. T lymphocyte numbers gradually increased following tumor progression, and subset analysis revealed an increase in the distribution of liver CD8+ and CD4+ T cells. Double staining for CD3 and TUNEL demonstrated T-cell apoptosis. Apoptotic T cells were more frequent in the HCC livers compared to the normal livers, and were spatially associated with intratumoral activated HSCs (tHSCs), suggesting a direct interaction. T-cell apoptosis was more frequently induced in the co-cultures of activated splenic T cells(aT)/tHSCs compared to aT/quiescent (q) HSCs or qT/tHSCs. tHSCs were positively correlated with T-cell apoptosis, and the percentage of T-cells undergoing apoptosis was positively correlated with the number of lung metastasis nodules. T-cell apoptosis may be promoted via an interaction with tHSCs, suggesting that tHSCs regulate T cells and contribute to lung metastasis in HCC.
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Use of twitch mouth pressure to assess diaphragm strength in patients with chronic obstructive pulmonary disease.
Respir Physiol Neurobiol
PUBLISHED: 04-09-2013
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This study was undertaken to determine whether twitch mouth pressure (TwPmo) can reliably assess diaphragm strength in patients with chronic obstructive pulmonary disease (COPD) using fully automatic trigger techniques. Fifteen patients with COPD were recruited. TwPmo, twitch oesophageal pressure (TwPes) and twitch transdiaphragmtic pressure (TwPdi) were generated by phrenic nerve stimulation and were measured using an inspiratory flow trigger (40 ml/s, Experiment 1) using an inspiratory pressure trigger (-5 cmH2O, Experiment 2) and using no trigger at functional residual capacity (Experiment 3). The correlation between TwPmo and TwPes was as follows: r=0.832; P<0.0001 (Experiment 1), r=0.900; P<0.0001 (Experiment 2); there was no significant correlation in Experiment 3. A Bland-Altman plot of the difference between TwPmo and TwPes showed the limits of agreement in Experiment (1) bias (range) 0.18 cmH2O (-2.05 to 2.41) and Experiment (2) bias (range) 0.32 cmH2O (-1.69 to 2.32). Measuring TwPmo using a fully automatic technique is a simple and convenient method for assessing diaphragm strength.
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Association between awareness of harmful effects of smoking and smoking cessation advice provided by hospital chest physicians in Guangzhou, China: a multi-institutional cross-sectional survey.
Respirology
PUBLISHED: 04-05-2013
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It is unknown which factors are associated with smoking cessation interventions initiated by hospital chest physicians in China. We examined physicians awareness of negative effects of smoking on smoking cessation advice given.
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Bis-triazolyl indoleamines as unique "off-approach-on" chemosensors for copper and fluorine.
Analyst
PUBLISHED: 04-04-2013
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Bis-triazolyl indoleamine-based chemosensors that respond to copper, and then fluorine as presumably facilitated by the high-affinity interaction between F(-) and the NH-proton of indole, are reported. Remarkable fluorimetric as well as colorimetric alternations upon the specific ligand-ion recognitions were observed.
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Capturing intercellular sugar-mediated ligand-receptor recognitions via a simple yet highly biospecific interfacial system.
Sci Rep
PUBLISHED: 03-19-2013
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Intercellular ligand-receptor recognitions are crucial natural interactions that initiate a number of biological and pathological events. We present here the simple construction of a unique class of biomimetic interfaces based on a graphene-mediated self-assembly of glycosyl anthraquinones to a screen-printed electrode for the detection of transmembrane glycoprotein receptors expressed on a hepatoma cell line. We show that an electroactive interface confined with densely clustered galactosyl ligands is able to ingeniously recognize the asialoglycoprotein receptors on live Hep-G2 cells employing simple electrochemical techniques. The only facility used is a personal laptop in connection with a cheap and portable electrochemical workstation.
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Transmembrane receptor CXCR7 increases the risk of extrahepatic metastasis of relatively well-differentiated hepatocellular carcinoma through upregulation of osteopontin.
Oncol. Rep.
PUBLISHED: 02-14-2013
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Recurrence and metastasis are the main obstacles to improving the survival of patients with post-resective hepato-cellular carcinoma (HCC). Our previous study suggests a critical role of CXCR7 in the metastasis of HCC. In the present study, the effect of CXCR7 as a risk factor for metastasis of HCC was evaluated. Immunohistochemical assay was performed on tissue microarrays based on HCC with extrahepatic metastases after hepatectomy. Two categories based on staining scores were used to evaluate the risk effect of CXCR7, respectively. The effect of CXCR7 on osteopontin (OPN) was explored by RNA interference. Based on the results, in both categories, highly expressed CXCR7 was a dependent risk factor for extrahepatic metastasis because of the potential association with relatively good cell differentiation. Stratification analyses indicated that CXCR7 was a strong independent risk factor (OR, 3.40; 95% CI, 1.07-18.84; P=0.038 in category 1 and OR, 6.40; 95% CI, 1.64-24.92; P=0.007 in category 2, respectively) in patients with Edmondson grade 1/2. Furthermore, CXCR7 correlated well and positively with expression of OPN (P=0.019 and P<0.001 in two categories, respectively) in HCC cases with Edmondson grade 1/2. Immunocytochemistry and RT-PCR demonstrated downregulation of OPN in a highly metastatic HCC cell line following knockdown of CXCR7. Taken together, these findings suggest that high expression of CXCR7 increases the risk of metastasis in post-resective HCC patients with relatively good differentiated tumors, potentially through upregulation of OPN. This group of patients may acquire a survival benefit from early detection and treatment of recurrence and metastasis.
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Fluorogenic probing of specific recognitions between sugar ligands and glycoprotein receptors on cancer cells by an economic graphene nanocomposite.
Adv. Mater. Weinheim
PUBLISHED: 01-14-2013
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Economical nanocomposites based on ?-stacking of N-acetyl glycosyl rhodamine B to graphene oxide (GO) are simply prepared. These "sweet" GO-materials are proven to be admirable for the fluorogenic recognition of specific intercellular sugar-based ligand-glycoprotein receptor interactions of interest.
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Effects of nebulized high-dose budesonide on moderate-to-severe acute exacerbation of asthma in children: a randomized, double-blind, placebo-controlled study.
Respirology
PUBLISHED: 01-02-2013
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The efficacy of inhaled corticosteroids (ICS) in asthma exacerbation are yet to be clarified. The aim of this study was to investigate the efficacy of nebulized ICS in children with moderate-to-severe acute exacerbation of asthma in an emergency room setting in order to elucidate the potential use of ICS as the first-line therapy in the management of acute exacerbation of asthma.
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Applying outdoor environment to develop health, comfort, and energy saving in the office in hot-humid climate.
ScientificWorldJournal
PUBLISHED: 01-01-2013
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A human life demand set to emerge in the future is the achievement of sustainability by maintaining a comfortable indoor environment without excessive reliance on energy-consuming air conditioners. The major research processes in this study are: (1) measuring indoor air quality and thermal comfort to evaluate the comfort of an indoor environment; (2) implementing questionnaire survey analysis to explore peoples environmental self-perceptions and conducting a meta-analysis of the measurement results for air quality and physical aspects; and (3) constructing an indoor monitoring and management system. The experimental and analysis results of this research reveal that most of the office occupants preferred a cooler environment with a lower temperature. Additionally, because the summers in Taiwan are humid and hot, the occupants of an indoor space tend to feel uncomfortable because of the high humidity and poor indoor air quality. Therefore, Variable Air Volume (VAV), two air intakes, and exhaust plant are installed to improve indoor environment. After improvement, a lower temperature (approximately 21.2-23.9°C) indirectly reduces humidity, thereby making the occupants comfortable. Increasing air velocity to 0.1 ~ 0.15?m/s, the carbon dioxide concentrations decrease below the requirement of the WHO. Ninety-five percent of the workers corresponded to the standard comfort zone after this improvement.
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What is Visualize?

JoVE Visualize is a tool created to match the last 5 years of PubMed publications to methods in JoVE's video library.

How does it work?

We use abstracts found on PubMed and match them to JoVE videos to create a list of 10 to 30 related methods videos.

Video X seems to be unrelated to Abstract Y...

In developing our video relationships, we compare around 5 million PubMed articles to our library of over 4,500 methods videos. In some cases the language used in the PubMed abstracts makes matching that content to a JoVE video difficult. In other cases, there happens not to be any content in our video library that is relevant to the topic of a given abstract. In these cases, our algorithms are trying their best to display videos with relevant content, which can sometimes result in matched videos with only a slight relation.