JoVE Visualize What is visualize?
Stop Reading. Start Watching.
Advanced Search
Stop Reading. Start Watching.
Regular Search
Find video protocols related to scientific articles indexed in Pubmed.
[Study on the discharge properties of xeon flash lamp and experimental measurement].
Guang Pu Xue Yu Guang Pu Fen Xi
PUBLISHED: 10-02-2014
Show Abstract
Hide Abstract
The Xenon flash lamp is a new type of light source for analytical instrument. The present paper analyzed the discharge process of xenon flash lamp, presented the discharge test system, and conducted experimental measurement of the voltage, current and optical pulse signal in the process of discharge. The results show that in the preliminary discharge, the free electron concentration was at a low level, so the energy was at a low level, then following the gas discharge, numerous free electrons formed in the lamp, resultin in the increase in the concentration of free electrons, therefore discharge current rised rapidly and voltage reduced. The lamp released photons to generate light pulse in the moment of ionic recombination, The pulse xenon lamp light energy output and spectral characteristic is related to electron energy in recombination and combination level of xenon, if the input energy and the energy consumption of the xenon lamp is inconsistent, it will lead to repeated capacitor charging and discharging and produce oscillation waveform. This paper is very useful for understanding the process of xenon lamp discharge, optimizing the driver circuit and the production of xenon flash lamp.
Related JoVE Video
SUMO-1 plays crucial roles for spindle organization, chromosome congression, and chromosome segregation during mouse oocyte meiotic maturation.
Mol. Reprod. Dev.
PUBLISHED: 07-30-2014
Show Abstract
Hide Abstract
Small ubiquitin-related modifier-1 (SUMO-1)-dependent modifications of many target proteins are involved in a range of intracellular processes. Previous studies reported the localization of SUMO-1 during oocyte meiosis, and that overexpression of Sentrin/SUMO-specific protease 2 (SENP2), a de-SUMOylation protease, altered SUMO-modified proteins, and caused defects in metaphase-II spindle organization. In this study, we detailed the consequences of SUMO-1-mediated SUMOylation by either inhibition of SUMO-1 or UBC9 with a specific antibody or their depletion by specific siRNA microinjection. Inhibition or depletion of SUMO-1 or UBC9 in germinal vesicle (GV)-stage oocytes decreased the rates of germinal vesicle breakdown and first polar body (PB1) extrusion; caused defective spindle organization and misaligned chromosomes; and led to aneuploidy in matured oocytes. Stage-specific antibody injections suggested that SUMO-1 functions before anaphase I during PB1 extrusion. Further experiments indicated that the localization of ?-tubulin was disordered after SUMO-1 inhibition, and that SUMO-1 depletion disrupted kinetochore-microtubule attachment at metaphase I. Moreover, SUMO-1 inhibition resulted in less-condensed chromosomes, altered localization of REC8 and securin, and reduced BUBR1 accumulation at the centromere. On the other hand, overexpression of SUMO-1 in GV-stage oocytes had no significant effect on oocyte maturation. In conclusion, our results implied that SUMO-1 plays crucial roles during oocyte meiotic maturation, specifically involving spindle assembly and chromosome behavior, by regulating kinetochore-microtubule attachment and the localization of ?-tubulin, BUBR1, REC8, and securin.
Related JoVE Video
[Progress in metal-organic frameworks].
Se Pu
PUBLISHED: 05-15-2014
Show Abstract
Hide Abstract
Metal-organic frameworks (MOFs) are a class of crystalline materials built from organic binding ligands and metal ions through self-assembly. Currently, MOFs have drawn a growing interest among the scientific teams of various fields. Compared with conventional inorganic porous materials, MOFs possess larger specific surface areas, higher porosity and diversity of structures and functions, thus many potential applications have been proposed in the domains of gas adsorption and separation, sensors, drug delivery, catalysis or others. The combinations of MOFs and other materials such as graphene oxide, magnetic nanoparticles have obvious advantages in adsorption and separation. The appearance of novel materials greatly promotes interdisciplinary developments such as organic chemistry, inorganic chemistry, coordination chemistry, materials chemistry, life science and computer science. This article reviews the progress of MOFs in recent years, including the characteristics of MOFs, advances at home and abroad, applications, central issues of compound MOFs and the prospects in the future.
Related JoVE Video
Preparation of mixed lanthanides-immobilized magnetic nanoparticles for selective enrichment and identification of phosphopeptides by MS.
Electrophoresis
PUBLISHED: 03-15-2014
Show Abstract
Hide Abstract
A new type of mixed lanthanides-immobilized (Tb(3+) , Tm(3+) , Ho(3+) , Lu(3+) ) magnetic nanoparticles, Fe3 O4 @TCPP-DOTA-M(3+) , was prepared with a particle size of approximately 30 nm. A model protein, ?-casein, and a protein mixture of ?-casein and BSA (1:100) were first used to test the phosphopeptide enrichment efficiency of the newly developed magnetic nanoparticles. For the model protein ?-casein, 19 phosphopeptides were identified with the newly developed materials. Even in the tryptic digest of ?-casein and BSA (1:100), 16 phosphopeptides were easily detected, suggesting that the novel materials possess high selectivity in phosphopeptide enrichment. To evaluate the phosphopeptide enrichment efficiency in a real biological sample, the materials were used to capture phosphopeptides in the tryptic digests of an extract of HeLa cells. In total, 9048 phosphopeptides corresponding to 2103 phosphoproteins were identified in a single mass spectrometric analysis, indicating the great potential of the new materials for practical applications. Compared with metal oxide-based enrichment methods, the newly developed materials are convenient to prepare and easy to handle, and they save time in the phosphopeptide enrichment procedure, making these materials a good choice for highly selective and sensitive phosphopeptide enrichment in future phosphoproteome analyses.
Related JoVE Video
Proteomic analysis of 'Zaosu' pear (Pyrus bretschneideri Rehd.) and its early-maturing bud sport.
Plant Sci.
PUBLISHED: 01-21-2014
Show Abstract
Hide Abstract
Maturation of fruits involves a series of physiological, biochemical, and organoleptic changes that eventually make fleshy fruits attractive, palatable, and nutritional. In order to understand the mature mechanism of the early-maturing bud sport of 'Zaosu' pear, we analyzed the differences of proteome expression between the both pears in different mature stages by the methods of a combination of two-dimensional electrophoresis (2-DE) and matrix assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) analysis. Seventy-five differential expressed protein spots (p<0.05) were obtained between 'Zaosu' pear and its early-maturing bud sport, but only sixty-eight were demonstratively identified in the database of NCBI and uniprot. The majority of proteins were linked to metabolism, energy, stress response/defense and cell structure. Additionally, our data confirmed an increase of proteins related to cell-wall modification, oxidative stress and pentose phosphate metabolism and a decrease of proteins related to photosynthesis and glycolysis during the development process of both pears, but all these proteins increased or decreased faster in the early-maturing bud sport. This comparative analysis between both pears showed that these proteins were closely associated with maturation and could provide more detailed characteristics of the maturation process of both pears.
Related JoVE Video
Different biosynthesis patterns among flavonoid 3-glycosides with distinct effects on accumulation of other flavonoid metabolites in pears (Pyrus bretschneideri Rehd.).
PLoS ONE
PUBLISHED: 01-01-2014
Show Abstract
Hide Abstract
Flavonoid biosynthesis profile was clarified by fruit bagging and re-exposure treatments in the green Chinese pear 'Zaosu' (Pyrus bretschneideri Rehd.) and its red mutant 'Red Zaosu'. Two distinct biosynthesis patterns of flavonoid 3-glycosides were found in 'Zaosu' pear. By comparison with 'Red Zaosu', the biosynthesis of flavonoid 3-galactosides and flavonoid 3-arabinosides were inhibited by bagging and these compounds only re-accumulated to a small degree in the fruit peel of 'Zaosu' after the bags were removed. In contrast, the biosynthesis of flavonoid 3-gluctosides and flavonoid 3-rutinosides was reduced by bagging and then increased when the fruits were re-exposed to sunlight. A combination of correlation, multicollinearity test and partial-correlation analyses among major flavonoid metabolites indicated that biosynthesis of each phenolic compound was independent in 'Zaosu' pear, except for the positive correlation between flavonoid 3-rutincosides and flavanols. In contrast with the green pear cultivar, almost all phenolic compounds in the red mutant had similar biosynthesis patterns except for arbutin. However, only the biosynthesis of flavonoid 3-galactosides was relatively independent and strongly affected the synthesis of the other phenolic compounds. Therefore, we propose a hypothesis that the strong accumulation of flavonoid 3-galactosides stimulated the biosynthesis of other flavonoid compounds in the red mutant and, therefore, caused systemic variation of flavonoid biosynthesis profiles between 'Zaosu' and its red mutant. This hypothesis had been further demonstrated by the enzyme activity of UFGT, and transcript levels of flavonoid biosynthetic genes and been well tested by a stepwise linear regression forecasting model. The gene that encodes flavonoid 3-galacosyltransferase was also identified and isolated from the pear genome.
Related JoVE Video
[Radioprotective effect of rmIL-12 on mice irradiated by ?-ray].
Zhongguo Shi Yan Xue Ye Xue Za Zhi
PUBLISHED: 09-04-2013
Show Abstract
Hide Abstract
The aim of this study was to investigate the radioprotective effect of recombinant murine interleukin 12 (rmIL-12) on mice irradiated by ?-ray. Fifty- six BALB/c mice were totally irradiated by 6.0 Gy of (60)Co ?-ray and randomly divided into irradiation control group,rmIL-12 treated group and recombinant murine thrombopoietin (rmTPO) treated group.The 5 and 20 µg/kg of rmIL-12 were administrated intraperitoneally at 24 h before irradiation respectively (low and high dose rmIL-12 treated group), 15 µg/kg of rmTPO was administrated subcutaneously at 30 min and 24 h following irradiation in rmTPO treated group. The general conditions of mice were observed twice a day, the changes in body weight and peripheral blood cell counts were examined once every three days, bone marrow cells were collected to perform colony cultivation at day 14 and 28 after irradiation. The results showed that the general conditions of mice in rmIL-12 treated group were better than those in irradiation control group. Compared with the irradiation control group,5 and 20 µg/kg rmIL-12 treatment significantly promoted platelet recovery, resulting in less profound nadirs (15.9% vs 8.1%,18.2% vs 8.1%,P < 0.01) and rapid recovery to normal levels (11 days vs 14 days). WBC count recovery rate in rmIL-12 treated group was faster than that in the irradiation control group. The WBC and platelet count recovery rate in 5 µg/kg rmIL-12 treated group were as fast as that in the rmTPO treated group, both of which were slower than that in 20 µg/kg rmIL-12 treated group (P > 0.05). Semi-solid bone marrow cell culture also demonstrated that rmIL-12 could stimulate bone marrow cells to form more CFU-Mix than those in the irradiation control group in vitro at day 14 and 28 after irradiation(P < 0.01).There was no significant difference between rmIL-12 and rmTPO treated groups (P > 0.05), CFU-GM counts in 5 µg/kg rmIL-12 treated group and rmTPO treated group at day 28 after irradiation were higher than those in irradiation control group(P < 0.05), but less than those in 20 µg/kg rmIL-12 treated group (P < 0.05). It is concluded that rmIL-12 has a significant radioprotective effect on mice irradiated by ?-ray.
Related JoVE Video
Bora regulates meiotic spindle assembly and cell cycle during mouse oocyte meiosis.
Mol. Reprod. Dev.
PUBLISHED: 04-16-2013
Show Abstract
Hide Abstract
Bora is the binding partner of Aurora A, which is required for its activation and phosphorylation of Polo like kinase 1 (Plk1), and is involved in the spindle assembly and progress of the cell cycle during mitosis. In this study, we examined the expression, localization, and function of Bora during mouse oocyte meiosis. The expression level of Bora was increased during oocyte meiotic maturation, with an elevated level at metaphase. Immunofluorescence analysis showed that Bora was concentrated as a dot shortly after germinal vesicle breakdown (GVBD), associating first with the surrounding chromosomes and then with the spindle throughout oocyte meiotic maturation. Further experiments confirmed that Bora co-localized with ?-tubulin at prometaphase/metaphase, but dissociated from ?-tubulin at anaphase/telophase. In metaphase-II-arrested oocytes, Bora was evenly distributed in the cytoplasm after treatment with a microtubule-depolymerizing agent, or recruited to the spindle after treatment with a microtubule-polymerizing agent, indicating that Bora was physically connected to the meiotic spindle and ?-tubulin at metaphase. Furthermore, inhibition or depletion of Bora by either anti-Bora antibody or Bora siRNA microinjection significantly reduced the rates of GVBD and inhibited first polar body extrusion; caused morphologically defective spindles and misaligned chromosomes; arrested maturing oocytes at prometaphase/metaphase-I stage, or left oocytes and their first polar bodies with severely misaligned chromosomes and defective spindles; and/or caused the disappearance of Aurora A and Plk1 at the spindle. These results indicated that Bora acts as a critical regulator of Aurora A and Plk1, and is involved in microtubule organization during oocyte meiosis.
Related JoVE Video
SUMOylation of mouse p53b by SUMO-1 promotes its pro-apoptotic function in ovarian granulosa cells.
PLoS ONE
PUBLISHED: 01-01-2013
Show Abstract
Hide Abstract
Follicular atresia is a process of spontaneous degradation of follicles, hindering growth and development in the mammalian ovary. Previous studies showed that follicular atresia was caused by apoptosis of granulosa cells, for which a number of apoptosis-related genes have already been identified. The roles of p53 in apoptosis of mouse granulosa cells and its post-translational modification are still unclear. The main objective of this study was to explore the roles of p53 in mouse granulosa cells. We found that mouse p53b, but not p53a, could be SUMOylated by SUMO-1 at lysine 375, which was essential for the protein stability of p53b in a dose-dependent manner. Immunofluorescent staining showed that wild p53b was located in the nucleus of granulosa cells, while its mutation of SUMOylated site (K375R) was localized in both nucleus and cytoplasm, implying that SUMOylation was necessary for the nuclear localization of p53b in granulosa cells. Overexpression of wild-type p53b, but not the mutation of SUMOylation site (K375R), significantly induced the expression of apoptosis-related gene, Bax, and increased the level of apoptosis in granulosa cells. This suggested that SUMO-1 modification of p53b was essential for inducing apoptosis in granulosa cells. Our results provide strong evidences that modification of p53b by SUMO-1 at lysine 375 was necessary for its activity to induce apoptosis in mouse granulosa cells, and it was involved in the regulation of p53b protein stability and nuclear localization. This implies that modification of p53b by SUMO-1 might regulate follicular atresia by inducing the apoptosis of ovarian granulosa cells in mice.
Related JoVE Video
[Endoscopic surgery and endoscopy-assisted surgery for the malignant transformation of sinonasal inverted papilloma].
Zhonghua Er Bi Yan Hou Tou Jing Wai Ke Za Zhi
PUBLISHED: 09-03-2011
Show Abstract
Hide Abstract
To investigate malignant transformation of sinonasal inverted papilloma (SIP) treated by nasal endoscopic and endoscopy-assisted surgery.
Related JoVE Video
[Value of intraoperative radiotherapy in the treatment of locally advanced pancreatic cancer].
Zhonghua Yi Xue Za Zhi
PUBLISHED: 03-23-2011
Show Abstract
Hide Abstract
To assess the value of intraoperative radiotherapy (IORT) in the combined treatment of locally advanced pancreatic cancer.
Related JoVE Video
Role of adjuvant radiotherapy for stage II thymoma after complete tumor resection.
Int. J. Radiat. Oncol. Biol. Phys.
PUBLISHED: 04-06-2010
Show Abstract
Hide Abstract
To determine whether patients with Masaoka stage II thymoma benefit from adjuvant radiation therapy after complete tumor resection.
Related JoVE Video
[Value of intraoperative electron radiation therapy in the treatment of malignant bone or soft tissue tumors around the joints].
Zhonghua Zhong Liu Za Zhi
Show Abstract
Hide Abstract
To evaluate the value of intraoperative radiation therapy with electrons (ELIOT) in treatment of malignant bone or soft tissue tumors around the joints.
Related JoVE Video
[The change of pulmonary surfactant protein of rat following silica exposure].
Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi
Show Abstract
Hide Abstract
To investigate the change of lung surfactant protein (SP) A,B,C,D of rats following silica dust exposure in order to provide the evidences for the early diagnosis indices or therapy of silicosis.
Related JoVE Video
[Physiological responses of Enteromorpha linza and Enteromorpha prolifera to seawater salinity stress].
Ying Yong Sheng Tai Xue Bao
Show Abstract
Hide Abstract
To investigate the physiological responses and adaptation mechanisms of Enteromorpha to seawater salinity stress, a laboratory experiment with Enteromorpha linza and E. prolifera was conducted to study their fresh mass (FM), relative growth rate (RGR), relative electrical conductivity (REC), chlorophyll (Chl) and carotenoid (Car) contents, Chl a/Chl b, Chl/Car, chlorophyll fluorescence parameters, and osmotic adjustment ability (OAA) under the stress of different salinity levels of diluted and concentrated seawater for 10 days. Compared with the control, 10%-200% salinity seawater increased the FM and RGR of the two Enteromorpha species obviously, 100% and 50% salinity seawater made the FM and RGR of E. linza and E. prolifera peaked, respectively, while 300% salinity seawater decreased the FM and RGR of E. linza and E. prolifera significantly, with the decrement being larger for E. linza. The biomass of E. linza and E. prolifer only had an increase in 50% and 100% sanity seawater and in 10%, 50%, 100%, and 200% salinity seawater, respectively. The Chl and Car contents and Chl a/Chl b of E. linza and E. prolifera had a significant increase in 10% salinity seawater, but decreased after an initial increase with the increasing salinity level of seawater. The Chl and Car contents and Chl a/Chl b of E. linza and E. prolifera peaked in 100% and 50% salinity seawater, respectively. With increasing salinity of seawater, the light use efficiency (alpha), maximal photochemical efficiency of PS II (F(v)/F(m)), actual photochemical efficiency of PS II in the light (Yield), maximal relative electron transport rate (rETR(max)), and half-saturation light intensity (I(k)) of E. linza and E. prolifera all showed the same variation trend as Chl. 10% -300% salinity seawater enabled E. linza and E. prolifera to express certain osmotic adjustment ability (OAA), and the OAA of E. linza and E. prolifer peaked in 100% and 50% salinity seawater, respectively. The growth of Enteromorpha had no correlation with Chl/Car, but was significantly negatively correlated with REC and positively correlated with Chl, Car, Chl a/ Chl b, F(v)/F(m), Yield, rETR(max), alpha, I(k), and OAA. To sum up, 100% salinity was the optimal salt concentration for the growth of E. linza, and 50% salinity was optimal for E. prolifera. E. prolifera could adapt to a wider range of salinity than E. linza. The parameters REC, Chl, Car, Chl a/Chl b, F(v)/F(m), Yield, rETR(max), alpha, I(k), and OAA could be used to evaluate the salt adaptation of Enteromorpha.
Related JoVE Video
[Therapeutic effect of rmIL-12 combined with G-CSF on acute radiation sickness produced by ?-ray irradiation in mice].
Zhongguo Shi Yan Xue Ye Xue Za Zhi
Show Abstract
Hide Abstract
The aim of this study is to observe the therapeutic effect of recombinant murine interleukin 12 (rmIL-12) combining with granulocyte colony stimulating factor (G-CSF) on mice irradiated by ?-rays. 56 BALB/c mice were totally irradiated by 6.0 Gy of (60)Co ?-ray and randomly divided into irradiation control group, rmIL-12 treatment group, G-CSF treatment group and combination therapy (rmIL-12 plus G-CSF) group. rmIL-12 20 µg/kg was administrated intraperitoneally at 1 h following irradiation, and was administrated every 3 days after irradiation for 4 times in rmIL-12 treatment group. G-CSF 100 µg/kg was administrated subcutaneously the 2 h following irradiation for 14 d in G-CSF treatment group. The dose and method of rmIL-12 and G-CSF in combination therapy group were same as in rmIL-12 group and G-CSF group. The general status of mice were observed twice a day, the changes in body weight, peripheral blood cell (WBC and Plt) counts were examined once every three days, bone marrow cells were collected to perform colony cultivation on day 14 and 28 after irradiation. The results showed that WBC count recovery time in combination therapy group was significantly earlier than that of the control group (7 d vs 11 d), WBC count recovery velocity in the combination therapy group was no significant different from that of the G-CSF treatment group. Combined therapy significantly promoted Plt count recovery, resulting in less profound nadirs (16.5% vs 8.1%, P < 0.01) and rapid recovery to normal levels (11 d vs 14 d), Plt count recovery velocity in the combination therapy group was no significant different from that of the rmIL-12 treatment group. Culture of bone marrow cells in semi-solid medium also demonstrated that combination of rmIL-12 and G-CSF could stimulate bone marrow cells to form more CFU-GM and CFU-Mix than those of the irradiation control group in vitro on day 14 and 28 after irradiation (P < 0.05). It is concluded that the combination of rmIL-12 and G-CSF can significantly accelerate the recovery of hematopoietic function in mice with acute radiation sickness.
Related JoVE Video
[Impact of pregnancy terminations before 28 weeks of gestation on the overall prevalence of neural tube defects in two counties of Shanxi province].
Zhonghua Liu Xing Bing Xue Za Zhi
Show Abstract
Hide Abstract
To examine the impact of pregnancy termination before 28 weeks of gestation on the overall prevalence of neural tube defects (NTDs).
Related JoVE Video
[Complications and short-term outcomes of intraoperative electron radiotherapy (IOERT) in patients with periarticular soft-tissue sarcomas of the extremities].
Zhonghua Zhong Liu Za Zhi
Show Abstract
Hide Abstract
To analyze the complications and short-term outcomes of patients receiving intraoperative electron radiotherapy (IOERT) for locally advanced and recurrent periarticular soft tissue sarcomas of the extremities.
Related JoVE Video
Hierarchical MnO? nanostructures: synthesis and their application in water treatment.
Water Sci. Technol.
Show Abstract
Hide Abstract
Hierarchical MnO? nanostructures were prepared through the reaction between KMnO? and oleic acid at room temperature in the surfactant-free microemulsion system. The obtained samples were characterized by powder X-ray diffraction, N? adsorption, scanning electron microscopy, and transmission electron microscopy. The results indicated that the flowerlike nanospheres were three-dimensional (3D) porous microstructures consisting of nanoplates. The surface area of the sample was 171.5 m(2)/g and the distribution of pore diameter lay within the range of 5-15 nm. The prepared hierarchically structured MnO? showed excellent adsorption capacity and rapid adsorption rate for methylene blue ions in water. The maximum adsorption capacity of methylene blue was as high as 273.9 mg/g and 97.5% of the dye was removed within initial 5 min of contact time. Compared with other adsorbents, the synthesized hierarchical MnO? nanostructures displayed a faster adsorption rate and higher adsorption capacity, which implied potential application for removing dye pollutants from waste water.
Related JoVE Video

What is Visualize?

JoVE Visualize is a tool created to match the last 5 years of PubMed publications to methods in JoVE's video library.

How does it work?

We use abstracts found on PubMed and match them to JoVE videos to create a list of 10 to 30 related methods videos.

Video X seems to be unrelated to Abstract Y...

In developing our video relationships, we compare around 5 million PubMed articles to our library of over 4,500 methods videos. In some cases the language used in the PubMed abstracts makes matching that content to a JoVE video difficult. In other cases, there happens not to be any content in our video library that is relevant to the topic of a given abstract. In these cases, our algorithms are trying their best to display videos with relevant content, which can sometimes result in matched videos with only a slight relation.