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Find video protocols related to scientific articles indexed in Pubmed.
Ion chromatography as highly suitable method for rapid and accurate determination of antibiotic fosfomycin in pharmaceutical wastewater.
Water Sci. Technol.
PUBLISHED: 05-22-2014
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A rapid and accurate ion chromatography (IC) method (limit of detection as low as 0.06 mg L(-1)) for fosfomycin concentration determination in pharmaceutical industrial wastewater was developed. This method was compared with the performance of high performance liquid chromatography determination (with a high detection limit of 96.0 mg L(-1)) and ultraviolet spectrometry after reacting with alizarin (difficult to perform in colored solutions). The accuracy of the IC method was established in the linear range of 1.0-15.0 mg L(-1) and a linear correlation was found with a correlation coefficient of 0.9998. The recoveries of fosfomycin from industrial pharmaceutical wastewater at spiking concentrations of 2.0, 5.0 and 8.0 mg L(-1) ranged from 81.91 to 94.74%, with a relative standard deviation (RSD) from 1 to 4%. The recoveries of effluent from a sequencing batch reactor treated fosfomycin with activated sludge at spiking concentrations of 5.0, 8.0, 10.0 mg L(-1) ranging from 98.25 to 99.91%, with a RSD from 1 to 2%. The developed IC procedure provided a rapid, reliable and sensitive method for the determination of fosfomycin concentration in industrial pharmaceutical wastewater and samples containing complex components.
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Ulinastatin activates the renin-angiotensin system to ameliorate the pathophysiology of severe acute pancreatitis.
J. Gastroenterol. Hepatol.
PUBLISHED: 01-28-2014
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Ulinastatin is a drug used effectively to alleviate symptoms and improve the pathophysiology of various types of pancreatitis. However, the molecular mechanism responsible for its action remains unknown. Therefore, we further explore the therapeutic effects of ulinastatin and investigate possible molecular pathways modulated by this drug in the development of severe acute pancreatitis (SAP).
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Identifying changes in dissolved organic matter content and characteristics by fluorescence spectroscopy coupled with self-organizing map and classification and regression tree analysis during wastewater treatment.
Chemosphere
PUBLISHED: 01-12-2014
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The stabilization of latent tracers of dissolved organic matter (DOM) of wastewater was analyzed by three-dimensional excitation-emission matrix (EEM) fluorescence spectroscopy coupled with self-organizing map and classification and regression tree analysis (CART) in wastewater treatment performance. DOM of water samples collected from primary sedimentation, anaerobic, anoxic, oxic and secondary sedimentation tanks in a large-scale wastewater treatment plant contained four fluorescence components: tryptophan-like (C1), tyrosine-like (C2), microbial humic-like (C3) and fulvic-like (C4) materials extracted by self-organizing map. These components showed good positive linear correlations with dissolved organic carbon of DOM. C1 and C2 were representative components in the wastewater, and they were removed to a higher extent than those of C3 and C4 in the treatment process. C2 was a latent parameter determined by CART to differentiate water samples of oxic and secondary sedimentation tanks from the successive treatment units, indirectly proving that most of tyrosine-like material was degraded by anaerobic microorganisms. C1 was an accurate parameter to comprehensively separate the samples of the five treatment units from each other, indirectly indicating that tryptophan-like material was decomposed by anaerobic and aerobic bacteria. EEM fluorescence spectroscopy in combination with self-organizing map and CART analysis can be a nondestructive effective method for characterizing structural component of DOM fractions and monitoring organic matter removal in wastewater treatment process.
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Angiotensin-converting enzyme (ACE and ACE2) imbalance correlates with the severity of cerulein-induced acute pancreatitis in mice.
Exp. Physiol.
PUBLISHED: 01-10-2014
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Angiotensin-converting enzyme (ACE) and its effector peptide angiotensin II (Ang II) have been implicated in the pathogenesis of pancreatitis. Angiotensin-converting enzyme 2 (ACE2) degrades Ang II to angiotensin-(1-7) [Ang-(1-7)] and has recently been described to have an antagonistic effect on ACE signalling. However, the specific underlying role of ACE2 in the pathogenesis of severe acute pancreatitis (SAP) is unclear. In the present study, the local imbalance of ACE and ACE2, as well as Ang II and Ang-(1-7) expression, was compared in wild-type (WT) and ACE2 knock-out (KO) or ACE2 transgenic (TG) mice subjected to cerulein-induced SAP. Serum amylase, tumour necrosis factor-?, interleukin (IL)-1?, IL-6 and IL-10 levels and histological morphometry were used to determine the severity of pancreatitis. In WT mice, pancreatic ACE and Ang II and serum Ang II expression increased (P < 0.05), while pancreatic ACE2 and Ang-(1-7) and serum Ang-(1-7) levels were also significantly elevated (P < 0.05) from 2 to 72 h after the onset of SAP. However, the ratio of pancreatic ACE2 to ACE expression was significantly reduced (from 1.46 ± 0.09 to 0.27 ± 0.05, P < 0.001) and paralleled the severity of pancreatitis. The Ace2 KO mice exhibited increased levels of tumour necrosis factor-?, IL-1?, IL-6, multifocal coagulative necrosis and inflammatory infiltrate, and lower levels of serum IL-10 and pancreatic Ang-(1-7) (4.70 ± 2.13 versus 10.87 ± 2.51, P < 0.001) compared with cerulein-treated WT mice at the same time point. Conversely, Ace2 TG mice with normal ACE expression were more resistant to SAP challenge as evidenced by a decreased inflammatory response, attenuated pathological changes and increased survival rates. These data suggest that the ACE2-ACE imbalance plays an important role in the pathogenesis of SAP and that pancreatic ACE2 is an important factor in determining the severity of SAP.
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Assessing removal efficiency of dissolved organic matter in wastewater treatment using fluorescence excitation emission matrices with parallel factor analysis and second derivative synchronous fluorescence.
Bioresour. Technol.
PUBLISHED: 05-20-2013
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To assess removal efficiency of dissolved organic matter (DOM) in the wastewater treatment plant (WWTP), fluorescence excitation emission matrices (EEM) with parallel factor analysis (PARAFAC) and second derivative synchronous fluorescence (SDSF) were used for the characterization of DOM in wastewater. In A(2)/O process, tryptophan-like and tyrosine-like materials were removed to the much greater extent than that of fulvic-like. The protein-like material might be mostly decomposed by anaerobic and aerobic bacteria in anaerobic/anoxic and oxic zones. C1, C2, I276, I286, AP1 and AP2 were much better in tracing variations of tryptophan-like and tyrosine-like materials than C3, I329 and AF1 in tracing fulvic-like. The number of sampling sites should be reduced, as concentration variations of DOM components were subtle among sampling sites in the oxic zone and secondary sedimentation tank. SDSF may be a useful tool as PARAFAC to monitor removal efficiency of DOM fractions from wastewater in the WWTP.
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Proteomic changes in the base of chrysanthemum cuttings during adventitious root formation.
BMC Genomics
PUBLISHED: 03-30-2013
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A lack of competence to form adventitious roots by cuttings of Chrysanthemum (Chrysanthemum morifolium) is an obstacle for the rapid fixation of elite genotypes. We performed a proteomic analysis of cutting bases of chrysanthemum cultivar Jinba during adventitious root formation (ARF) in order to identify rooting ability associated protein and/or to get further insight into the molecular mechanisms controlling adventitious rooting.
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Chrysanthemum cutting productivity and rooting ability are improved by grafting.
ScientificWorldJournal
PUBLISHED: 01-01-2013
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Chrysanthemum has been commercially propagated by rooting of cuttings, whereas the quality will decline over multiple collections from a single plant. Therefore, we compared the vigour, rooting ability, and some physiological parameters between cuttings harvested from nongrafted "Jinba" (non-grafted cuttings) with those collected from grafted "Jinba" plants onto Artemisia scoparia as a rootstock (grafted cuttings). The yield, length, node number, stem diameter, fresh weight, and dry weight of the grafted cuttings were superior to the non-grafted cuttings. Also grafted cuttings "Jinba" rooted 1 day earlier, but showing enhanced rooting quality including number, length, diameter, and dry weight of roots, where compared to the non-grafted. The physiological parameters that indicated contents of soluble protein, peroxidase activity, soluble sugar, and starch, ratios of soluble sugar/nitrogen ratio, and carbohydrate/nitrogen (C/N), as well as contents of indole-3-acetic acid (IAA) and abscisic acid (ABA), and IAA/ABA ratio were significantly increased in the grafted cuttings. This suggested their important parts in mediating rooting ability. Results from this study showed that grafting improved productivity and rooting ability related to an altered physiology, which provide a means to meet the increasing demand.
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Colloidal noble-metal and bimetallic alloy nanocrystals: a general synthetic method and their catalytic hydrogenation properties.
Chemistry
PUBLISHED: 04-23-2010
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A general single-step strategy has been developed for the direct thermal decomposition of noble-metal salts in octadecylamine to synthesize octahedron- and rod-shaped noble-metal aggregates and monodisperse noble-metal or bimetallic alloy nanocrystals without introducing any additive into the system. It has presented a facile and economic way to fabricate these nanocrystals, especially alloy nanocrystals, which does not require a post-synthesis solid-state annealing process. The morphology of the nanocrystals can be easily controlled by tuning the synthetic temperature. Their ability to catalyze heterogeneous Suzuki coupling reactions has been investigated and showed satisfactory catalytic activity. The catalytic performance of the monometallic and bimetallic alloy nanocrystals were also evaluated in the selective hydrogenation of citral in a conventional organic solvent (toluene) and a green solvent (supercritical carbon dioxide, scCO(2)). Interestingly, the catalysts performed differently to each other when they were in scCO(2) owing to the different morphology, which should be readily optimized for further use.
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Facile synthesis of porous Fe7Co3/carbon nanocomposites and their applications as magnetically separable adsorber and catalyst support.
Langmuir
PUBLISHED: 04-08-2010
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A facile co-gelation route has been developed to synthesize novel porous Fe(7)Co(3)/carbon composites with Fe(7)Co(3) nanoparticles embedded in the porous carbon matrix. The sol-gel process of this route simultaneously involves the hydrolysis of tetraethylorthosilicate (TEOS) and the polymerization of furfuryl alcohol (FA) within an ethanol solution containing TEOS, FA, and metal nitrates, which led to the inorganic/organic hybrid xerogel, accompanying metal salts spontaneously captured in the xerogel, mostly in the framework of poly(furfuryl alcohol) (PFA). Compared to the nanocasting route, the advantage of this method is that the formation of silica template and the impregnation of carbon precursor and metal salts were simultaneously carried out in one co-gelation process, which makes the synthesis very simple and eliminates the time-consuming synthesis of the silica template and multistep impregnation process. Different amounts of Fe(7)Co(3) can be introduced into the composites, which led to different pore structures and magnetic properties. The composites have large surface areas (as high as 651.4 m(2)/g) and high saturation magnetizations (as high as 31.2 emu/g). The Fe(7)Co(3)/carbon composites prepared were successfully applied to the removal of dyes from water and catalysis of hydrogenation as efficient magnetically separable adsober and catalyst support. The facile co-gelation route makes the scalable synthesis of magnetic porous carbon possible for application, and it also provides a promising path to the synthesis of nanoscale metal or alloy embedded in the porous carbon materials.
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Severe acute pancreatitis is associated with upregulation of the ACE2-angiotensin-(1-7)-Mas axis and promotes increased circulating angiotensin-(1-7).
Pancreatology
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Angiotensin-converting enzyme 2 (ACE2), its product angiotensin-(1-7) and its receptor Mas may counteract the adverse effects of the ACE-angiotensin receptor II-AT(1) axis in many diseases. We examined the expression of these novel components of the rennin-angiotensin system in an experimental mouse model of severe acute pancreatitis (SAP).
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Fractionation and characterization of dissolved extracellular and intracellular products derived from floccular sludge and aerobic granules.
Bioresour. Technol.
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Dissolved extracellular and intracellular polymeric substances (DEPS and DIPS) from floccular sludge and aerobic granules were extracted and fractionated based on compound hydrophobicity. Compared with floccular sludge, aerobic granules contained lower amounts of DEPS and DIPS. Fourier transforms infrared spectra suggested that large amounts of proteins and polysaccharides existed in colloidal form in the extracellular polymeric substances of aerobic granules. Fluorescence excitation-emission matrix spectra revealed that tightly bound DEPS (TB-DEPS) and DIPS were humified after sludge granulation. Meanwhile, the proportions of hydrophilic contents in the TB-DEPS and DIPS fractions decreased from 45.3% to 28.3% and from 40.1% to 18.9%, respectively. These data indicated that TB-DEPS and DIPS from aerobic granules were more hydrophobic than those from floccular sludge. The results of the hierarchical cluster analysis further confirmed that the characteristics of dissolved organic matters between floccular and granular sludge were distinctly different.
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SnoN as a key regulator of the high glucose-induced epithelial-mesenchymal transition in cells of the proximal tubule.
Kidney Blood Press. Res.
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Ski-related protein N (SnoN) suppression is essential to transforming growth factor-?1 induction and the epithelial-mesenchymal transition (EMT) in several cancer cells. The role of SnoN in diabetic nephropathy is unknown. We aimed to determine the role of SnoN in the EMT of proximal tubule cells (PTCs) maintained under high glucose conditions.
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In situ FTIR study on the formation and adsorption of CO on alumina-supported noble metal catalysts from H2 and CO2 in the presence of water vapor at high pressures.
Phys Chem Chem Phys
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The formation and adsorption of CO from CO(2) and H(2) at high pressures were studied over alumina-supported noble metal catalysts (Pt, Pd, Rh, Ru) by in situ FTIR measurements. To examine the effects of surface structure of supported metal particles and water vapor on the CO adsorption, FTIR spectra were collected at 323 K with untreated and heat (673 K) treated catalysts in the absence and presence of water (H(2)O, D(2)O). It was observed that the adsorption of CO occurred on all the metal catalysts at high pressures, some CO species still remained adsorbed under ambient conditions after the high pressure FTIR measurements, and the frequencies of the adsorbed CO species were lower either for the heat treated samples or in the presence of water vapor. It is assumed that the CO absorption bands on atomically smoother surfaces appear at lower frequencies and that water molecules are adsorbed more preferentially on atomically rough surfaces rather than CO species.
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JoVE Visualize is a tool created to match the last 5 years of PubMed publications to methods in JoVE's video library.

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We use abstracts found on PubMed and match them to JoVE videos to create a list of 10 to 30 related methods videos.

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In developing our video relationships, we compare around 5 million PubMed articles to our library of over 4,500 methods videos. In some cases the language used in the PubMed abstracts makes matching that content to a JoVE video difficult. In other cases, there happens not to be any content in our video library that is relevant to the topic of a given abstract. In these cases, our algorithms are trying their best to display videos with relevant content, which can sometimes result in matched videos with only a slight relation.