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Find video protocols related to scientific articles indexed in Pubmed.
[Remudy.]
Brain Nerve
PUBLISHED: 11-20-2014
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Abstract Remudy (Registry of Muscular Dystrophy), operated by NCNP (the National Center of Neurology and Psychiatry), Japan, runs two national registries for Dystrophinopathy and GNE myopathy in collaboration with the TREAT-NMD (Translational Research in Europe--Assessment and Treatment of Neuromuscular Disease) alliance. The aim of Remudy is to construct a clinical research infrastructure that accelerates the pace of clinical development research for these rare diseases. We successfully provide data sets for feasibility studies, send out appropriate information about clinical trials to speed up the recruitment of candidates, as well as present the natural history and epidemiology data of these rare diseases using a new 'registry based' research style. Remudy presents a prototype model of the clinical research infrastructure to overcome these rare and incurable diseases.
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Renal redox dysregulation in AKI: Application for oxidative stress marker of AKI.
Am. J. Physiol. Renal Physiol.
PUBLISHED: 10-29-2014
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Oxidative stress is a major determinant of acute kidney injury (AKI); however, the effects of the AKI on renal redox system are unclear and few existing AKI markers are suitable for evaluating oxidative stress. We measured urinary levels of the redox regulatory protein thioredoxin 1 (TRX1) in patients with various kinds of kidney disease and in mice with renal ischemia/reperfusion injury. Urinary TRX1 levels were markedly higher in patients with AKI than in those with chronic kidney disease or in healthy subjects. In a receiver operating characteristic curve analysis to differentiate between AKI and other renal diseases, the area under the curve for urinary TRX1 was 0.94 (95% confidence interval, 0.90-0.98), and the sensitivity and specificity were 0.88 and 0.88, respectively, at the optimal cutoff value of 43.0 ?g/g creatinine. Immunostaining revealed TRX1 to be diffusely distributed in the tubules of normal kidney, but to be shifted to the brush borders or urinary lumen in injured tubules in both mice and humans with AKI. Urinary TRX1 in AKI was predominantly in the oxidized form. In cultured human proximal tubular epithelial cells, hydrogen peroxide specifically and dose-dependently increased TRX1 levels in the culture supernatant, while decreasing intracellular levels. These findings suggest that urinary TRX1 is an oxidative stress-specific biomarker useful in distinguishing AKI from chronic kidney disease and healthy kidneys.
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Decreased extension gap and valgus alignment after implantation of total knee prosthesis in primary varus knees.
Knee Surg Sports Traumatol Arthrosc
PUBLISHED: 10-14-2014
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It was hypothesised that implantation of a total knee prosthesis may change the size and shape of the joint gap. To test this hypothesis, a tensor device was used which was specifically designed to reproduce the conditions before and after implantation, including attachment of the polyethylene insert trial. This study aimed to compare the joint gaps before and after implantation of a total knee prosthesis using this new tensor device.
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Preference of surgical procedure for the forefoot deformity in the rheumatoid arthritis patients-A prospective, randomized, internal controlled study.
Mod Rheumatol
PUBLISHED: 10-09-2014
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Objectives. The deformed rheumatoid forefoot may be treated with resection of lesser metatarsal heads combined with arthrodesis or resection of the first metatarsophalangeal joint. Recurrent hallux valgus deformity has been reported by resection. We performed a prospective, randomized, internal-controlled study to compare results between arthrodesis and resection. Methods. We resected the lesser metatarsal heads bilaterally and performed arthrodesis of the first metatarsophalangeal joint on one side and resection on the opposite side. We investigated 26 patients (52 feet) who were followed at least one year. Patients were assessed for clinical score, hallux valgus angle (HVA), angle between first and second metatarsals, and angle between first and fifth metatarsals preoperatively, postoperatively and at final follow-up. We evaluated callosities, claw toes, recurrences, and procedure preferences. Results. The mean follow-up period was 4.1 years. No significant differences between arthrodesis and resection were seen, with the exception of HVA. That was significantly less on arthrodesis side (11.5°) than on resection side (17.0°, p < 0.05). Seven callosities on resection side and four on arthrodesis side were observed. On resection side, hallux valgus deformity often recurred (15.3%). Patients expressed a significant preference for arthrodesis over resection (p = 0.008). Conclusions. Arthrodesis provides better results for maintaining HVA.
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Factors associated with periesophageal vagal nerve injury after pulmonary vein antrum isolation.
J Am Heart Assoc
PUBLISHED: 09-25-2014
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Periesophageal vagal nerve injury is recognized as a rare complication in atrial fibrillation ablation procedures. We investigated the factors associated with the occurrence of symptomatic periesophageal vagal nerve injury after pulmonary vein antrum isolation.
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Role of extended external auto-triggered loop recorder monitoring for atrial fibrillation.
Circ. J.
PUBLISHED: 09-22-2014
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Clinical outcomes after atrial fibrillation (AF) ablation are evaluated using standard 24-h Holter monitoring, and the large spontaneous variability of AF episodes and incidence of silent AF are major limitations. Further, symptoms generally decrease after AF ablation.Methods?and?Results:Newly developed extended external auto-trigger loop recorders (ELR) were used for 14-day consecutive monitoring to detect atrial tachyarrhythmia (ATa). Continuous tracings were stored for the initial 24h. Among 500 examinations after AF ablation in 342 patients, 40 ATa episodes were manually detected in 25 patients during the initial 24h. All episodes including 27 asymptomatic episodes (67.5%) were successfully identified using ELR. Recurrent ATa after AF ablation were detected in 83 patients, and a median monitoring duration of 4.0 days (IQR, 1.0-7.75 days) was required to detect the first episode of recurrence. The sensitivity of 24-h monitoring in detecting arrhythmia recurrence was 27.7% relative to the 14-day monitoring. The diagnostic yield gradually improved with longer monitoring duration regardless of the period after the ablation procedure. Longer follow-up, however, was required to obtain similar diagnostic yield >1 year after as compared to <1 year after the procedure.
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Evaluation of Diaphragmatic Electromyograms in Radiofrequency Ablation of Atrial Fibrillation: Prospective Study Comparing Different Monitoring Techniques.
J. Cardiovasc. Electrophysiol.
PUBLISHED: 08-25-2014
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The utility of compound motor action potential (CMAP) monitoring for anticipating phrenic nerve injury (PNI) during cryoballoon ablation has been reported. We sought to compare two different CMAP recording techniques and evaluated the feasibility during pulmonary vein antrum isolation (PVAI) and superior vena cava isolation (SVCI) using radiofrequency energy.
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Discriminating imaging findings of acute osteoporotic vertebral fracture: a prospective multicenter cohort study.
J Orthop Surg Res
PUBLISHED: 08-24-2014
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Appropriate treatment of osteoporotic vertebral fractures (OVF) requires knowledge of the age of the fracture. Although diagnostic imaging has made remarkable progress in recent years, it remains difficult to differentiate acute fractures from old. Our purpose was to investigate chronological changes in radiological findings after OVF and to identify discriminators of acute versus older injuries.
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Effect of selenite on T-cell mitogenesis: contribution of ROS production and apoptosis signal-regulating kinase 1.
Biol. Pharm. Bull.
PUBLISHED: 08-05-2014
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Although supplementation with the selenocompound, sodium selenite has been shown to stimulate the concanavalin A-induced T-cell mitogenic response, the mechanisms responsible remain unclear. This study was conducted to evaluate the relationships between the induction of apoptosis, formation of tumor necrosis factor (TNF)-alpha and reactive oxygen species (ROS), activation of apoptosis signal-regulating kinase (ASK) 1 and the thioredoxin (Trx) system when mitogenesis was stimulated by selenite. TNF-alpha was dose-dependently released by mouse splenocytes treated with selenite, and apoptosis was induced when TNF-alpha was added at the indicated concentrations. However, supplementation with selenite at low concentrations inhibited the accumulation of ROS with the increased expression of Trx reductase 1 and induction of apoptosis in wild-type splenocytes, and also at high concentrations in Trx-1-transgenic mouse splenocytes. The suppression of apoptosis was accompanied by a decrease in the expression of phospho-ASK1. These results suggest that the stimulation of T-cell mitogenesis by selenite may be partly attributed to the inhibited accumulation of ROS due to a reduced Trx-1/TR1 system, the inactivation of ASK1, and the suppression of apoptosis.
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Contrast-enhanced color Doppler ultrasonography increases diagnostic accuracy for soft tissue tumors.
Oncol. Rep.
PUBLISHED: 08-04-2014
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Resolution of ultrasonography (US) has undergone marked development. Additionally, a new-generation contrast medium (Sonazoid) used for US is newly available. Contrast-enhanced US has been widely used for evaluating several types of cancer. In the present study, we evaluated the ability of color Doppler US (CDUS) and Sonazoid to differentiate between benign and malignant soft tissue tumors. A total of 180 patients (87 male, 93 female) were enrolled in the present study. The patient ages ranged from 1 to 91 years (mean 58.1±20.0 years). The maximum size, depth, tumor margins, shape, echogenicity and textural pattern were measured on gray-scale images. CDUS was used to evaluate the intratumoral blood flow with and without Sonazoid. Peak systolic flow velocity (Vp), mean flow velocity (Vm), resistivity index (RI) and pulsatility index (PI) of each detected intratumoral artery were automatically calculated with power Doppler US (PDUS). The present study included 118 benign and 62 malignant tumors. Statistical significances were found in size, depth, tumor margin and textural pattern but not in shape or echogenicity on gray-scale images. Before Sonazoid injection, CDUS findings showed 55% sensitivity, 77% specificity and 69% accuracy, whereas contrast-enhanced CDUS showed 87% sensitivity, 68% specificity and 74% accuracy. There were no statistically significant differences between malignant and benign tumors regarding the mean Vp, Vm, RI and PI values determined on PDUS. In conclusion, contrast-enhanced CDUS proved to be a reliable diagnostic tool for detecting malignant potential in soft tissue tumors.
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Catheter ablation of atrial tachyarrhythmias causing inappropriate implantable cardioverter-defibrillator shocks.
Europace
PUBLISHED: 07-26-2014
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Inappropriate shocks have been an important issue post-implantable cardioverter-defibrillator (ICD) implantation. Moreover, inappropriate ICD shocks are associated with increased mortality. The objective of this study was to evaluate the feasibility of catheter ablation therapy for atrial tachyarrhythmias (ATa) responsible for inappropriate ICD shocks.
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Knee reconstruction with endoprosthesis after extra-articular and intra-articular resection of osteosarcoma.
Jpn. J. Clin. Oncol.
PUBLISHED: 07-23-2014
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Extra-articular resection is necessary to obtain a wide margin for primary osteosarcomas invading the knee joint, and the limb is often reconstructed using a prosthesis. Here, outcomes of extra-articular and intra-articular procedures were compared.
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Fate, origin and roles of cells within free bone grafts.
J Orthop Sci
PUBLISHED: 07-05-2014
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The efficacy of autologous bone grafting in repairing nonunion fractures, large bone defects and spinal instability is widely accepted. However, the cellular and molecular mechanisms underlying new bone formation in bone grafting have yet to be fully elucidated. The purpose of this study was to clarify the fate, origin and the contribution of the cells within the grafted bone.
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Pre-chemotherapy preservation of fertility in male patients with high-grade malignant bone and soft tissue tumors.
Mol Clin Oncol
PUBLISHED: 06-24-2014
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Only a limited number of orthopedic oncologists make arrangements for pre-chemotherapy fertility preservation, such as sperm cryopreservation, for their patients. The purpose of this study was to offer fertility preservation to male patients with high-grade malignant bone and soft tissue tumors and assess the outcomes. The study included 14 male patients, aged <45 years, with high-grade bone and soft tissue tumors. The median age at diagnosis was 23.0±12.0 years (range, 8-42 years). Following pathological confirmation of high-grade malignant tumor, we informed all the patients and/or their guardians on the issue of chemotherapy-related male infertility. If the patients were interested in preserving fertility, they were referred to a team of reproductive specialists. We documented the patients' clinical characteristics and techniques used for fertility preservation. The majority of unmarried and childless patients were interested in fertility preservation. Four patients (28.5%) selected sperm cryopreservation and 1 patient (7.1%) selected hemi-testicular preservation, as he had developed erectile dysfunction following previous surgery for colon cancer. Married patients and those with children did not wish to preserve fertility. In conclusion, infertility following chemotherapy raises serious concerns for young male patients with high-grade bone and soft tissue tumors. Prior to initiating chemotherapy, the potential risks of chemotherapy should be explained, counseling should be provided and informed consent should be obtained from the patients, ideally without delaying commencement of cancer treatment. Prior to administering cancer chemotherapy, orthopedic oncologists are encouraged to offer sperm banking to young male patients at risk of infertility.
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Severe low back pain in patients with rheumatoid arthritis is associated with Disease Activity Score but not with radiological findings on plain X-rays.
Mod Rheumatol
PUBLISHED: 06-20-2014
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Objective. To investigate the prevalence and associated factors of severe low back pain (LBP) among patients with rheumatoid arthritis (RA). Methods. This cross-sectional study included 201 patients with RA without prior spinal surgery. Severe LBP was defined as that with a visual analog scale (VAS) score of ? 50 mm within the previous 4 weeks. Lumbar lesions, sagittal alignment, and disc degeneration were evaluated by plain standing X-rays and magnetic resonance imaging. Associated factors of severe LBP were evaluated using multiple logistic regression analysis. Results. Forty-eight patients (23.8%) had LBP with a VAS score of ? 50 mm. Multivariate analysis indicated that the associated factors for severe LBP were female, smoking, and moderate and high disease activity on the Disease Activity Score in 28 joints-erythrocyte sedimentation rate (DAS28-ESR). There was no relationship between severe LBP and any radiological findings. Among DAS28-ESR subscores, patients with severe LBP had significantly higher tender joint counts and VAS scores for general health. Conclusions. The prevalence of severe LBP was relatively high in patients with RA. The factor most closely associated with severe LBP was Disease Activity Score, but not radiological findings. Severe LBP was related to the tender joint count or subjective complaints of RA.
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Protective effects of oral administration of yeast thioredoxin against gastric mucosal injury.
Biosci. Biotechnol. Biochem.
PUBLISHED: 06-18-2014
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Thioredoxin (TRX) is a redox regulating protein which has protective effects against oxidative stress-induced damage to cells and tissues. In this study, we investigated the effects of orally administered TRX derived from edible yeast, Saccharomyces cerevisiae, on gastric mucosa. First, we examined the digestibility of orally administered yeast TRX in mice, and detected yeast TRX in the stomach for 4?h after administration. Next, we investigated the mitigation of gastric mucosal injury after the oral administration of yeast TRX in water-immersion restraint stress and HCl/ethanol-induced gastric ulcer models. Furthermore, we conducted DNA microarray analysis, using the HCl/ethanol-induced model, which revealed that several groups of genes related to tissue repair were upregulated in ulcer regions in the stomachs of rats administered with yeast TRX. These results demonstrated the viability of the use of oral administrations of yeast TRX to protect the gastric mucosa.
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Osteosarcoma arising from the parapharyngeal space: A case report.
Oncol Lett
PUBLISHED: 06-18-2014
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The current study presents a rare case of osteoblastic osteosarcoma arising from an extremely rare site, namely, the parapharyngeal space. To the best of our knowledge, this is the first study of computed tomography (CT) and magnetic resonance imaging (MRI) of an osteosarcoma in the parapharyngeal space with pathological correlation. A 56-year-old male presented with a mass of the right facial region. CT and MRI showed a heterogeneous mass, with ossification or calcification, occupying the parapharyngeal space. Open biopsy revealed an osteoblastic osteosarcoma containing calcified malignant osteoid. Lung CT also showed multiple lung metastases at the time of the first visit to the Department of Orthopedic Surgery, Osaka City University Graduate School of Medicine (Osaka, Japan). Systemic chemotherapy and radiotherapy were administered to the patient for palliation. The patient was alive at the 24-month follow-up subsequent to this treatment. Although a definitive diagnosis requires the use of a biopsy, the CT and MRI findings described in the present study suggest inclusion of this rare tumor in the differential diagnosis that is formed when such findings occur in the parapharyngeal space. The present study also briefly discusses osteosarcoma of the parapharyngeal space.
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Nationwide patient registry for GNE myopathy in Japan.
Orphanet J Rare Dis
PUBLISHED: 05-28-2014
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GNE myopathy is a slowly progressive autosomal recessive myopathy caused by mutations in the GNE (glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase) gene. This study aimed to (1) develop a nationwide patient registry for GNE myopathy in order to facilitate the planning of clinical trials and recruitment of candidates, and (2) gain further insight into the disease for the purpose of improving therapy and care.
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Factors predicting an arrhythmogenic superior vena cava in atrial fibrillation ablation: insight into the mechanism.
Heart Rhythm
PUBLISHED: 04-30-2014
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The superior vena cava (SVC) is an infrequent but important source of atrial fibrillation (AF), but is not always easy to identify.
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Curative resection of gallbladder cancer with liver invasion and hepatic metastasis after chemotherapy with gemcitabine plus S-1: report of a case.
World J Surg Oncol
PUBLISHED: 04-19-2014
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A 62-year-old woman diagnosed with gallbladder cancer exhibiting broad liver invasion and metastasis to Couinaud's hepatic segments 4 and 8 (S4 and S8) consulted her regular doctor. Owing to the presence of liver metastases, she received treatment with gemcitabine plus S-1. After four cycles of chemotherapy, the size of the main lesion dramatically decreased and the two liver metastases disappeared. After six cycles of chemotherapy, the patient was referred to our hospital for surgical treatment. Upon admission, there was no evidence of any distant metastasis, based on a detailed radiological examination. Therefore, we performed cholecystectomy and central bisegmentectomy of the liver after obtaining the patient's informed consent. Pathological examination demonstrated viable cancer cells with granuloma formation and calcification in the gallbladder, as well as regenerative changes without viable cancer cells in S4 and S8 of the liver. Gemcitabine plus S-1 was again administered as postoperative adjuvant chemotherapy. One and a half years after the surgery, there were no signs of recurrence. In patients selected according to their response to chemotherapy, surgical treatment might therefore be effective against gallbladder cancer with metastasis.
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Intraoperative assessment of midflexion laxity in total knee prosthesis.
Knee
PUBLISHED: 04-07-2014
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Soft-tissue balancing of the knee is fundamental to the success of a total knee arthroplasty (TKA). In posterior-stabilized TKA, there is no stabilizer of the anterior-posterior translation in the midflexion range in which the cam-post mechanism does not engage yet. Therefore, instability in the midflexion range is suspected to occur in posterior-stabilized TKA. The purpose of this study was to measure the joint gap throughout a full range of motion and to analyze the joint gap laxity in the midflexion range after implantation of a mobile-bearing posterior-stabilized total knee prosthesis.
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Detection of Small Periprosthetic Bone Defects after Total Knee Arthroplasty.
J Arthroplasty
PUBLISHED: 03-31-2014
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Large bone defect around total knee prostheses is among the most critical challenges in revision surgery. However, it is difficult to detect bone defects around a prosthesis in early stage. We compared the efficacy of the detection of small bone defects between fluoroscopically guided plain radiography, CT, MRI, and a novel tomographic technique (tomosynthesis) using the six pig knee models. No bone defects were detected with plain radiography and MRI. The sensitivity and specificity of CT were 61.5% and 64.1%, respectively. The sensitivity and specificity of tomosynthesis were 85.4% and 87.2%, respectively. The radiation dose of tomosynthesis was 6% of that of CT. The cost of tomosynthesis was 28% of that of CT. Tomosynthesis was superior in terms of diagnosis, radiation dose, and cost.
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Current prevalence and characteristics of cervical spine instability in patients with rheumatoid arthritis in the era of biologics.
Mod Rheumatol
PUBLISHED: 03-26-2014
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Abstract Objectives. Cervical spine instability (CSI) is commonly involved in patients with rheumatoid arthritis (RA). Although the treatment for RA has dramatically changed due to methotrexate and biologics, it is unclear whether this change contributes to the prevalence of CSI or not. Our objectives were to update the current prevalence of CSI and to investigate the factors associated with CSI. Methods. A cross-sectional study of patients with RA was conducted in our outpatient clinic. Clinical information and symptoms related to CSI were obtained. Plain radiography and magnetic resonance imaging were performed. CSI included atlantoaxial subluxation (AAS), vertical subluxation (VS), and subaxial subluxation (SAS). Results. Two hundred and twenty patients were analyzed, 93 (42%) of whom had CSI. A ? 10-year disease duration, Steinbrocker stage III, and three or more narrowed disc spaces from C2/3 to C6/7 were significantly associated with CSI. A neck pain VAS was associated with VS, but not with AAS and SAS. In contrast, methotrexate and biologics had no effect on CSI. Conclusion. The prevalence of CSI in this study was lower compared to previous reports before the approval of biologic, although we failed to detect the effect of biologics. Further prospective studies are needed to elucidate the efficacy.
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Diagnosis of osteosarcoma in a patient previously treated for Ewing sarcoma.
Skeletal Radiol.
PUBLISHED: 03-20-2014
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Primary malignant bone tumors, whether Ewing sarcoma or osteosarcoma, are a rare type of tumor. The sequential occurrence of two bone sarcomas, Ewing sarcoma and high-grade osteosarcoma, in the same patient at two different locations is an exceptionally rare phenomenon. We present the case of a 13-year-old girl who presented with a high-grade osteoblastic osteosarcoma of the distal femur, 7 years after treatment for Ewing sarcoma of the left pelvis. She did not receive radiation therapy. Following the recent developing multidisciplinary therapy, long-term follow-up for monitoring latent treatment-related adverse effects may be necessary for survivors of primary malignant bone tumors.
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Circulating nucleated peripheral blood cells contribute to early-phase meniscal healing.
J Tissue Eng Regen Med
PUBLISHED: 03-10-2014
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The purpose of this study was to assess how peripheral blood cells (PBCs) contribute to meniscus repair, using a parabiotic rat model. Wild-type (WT) and green fluorescent protein (GFP) transgenic rats were conjoined at the torso. After 4 weeks, the anterior part of the medial meniscus of both groups of rats was removed. At 1, 2, 4, 8 and 12 weeks post-meniscectomy, repaired tissue was evaluated using stereomicroscopy, histology with toluidine blue staining, and immunofluorescence microscopy. Stereomicroscopic observations and confocal laser microscopy revealed that a high number of GFP-positive cells were present in the repaired meniscus of WT rats 1 week post-meniscectomy, and the number of GFP-positive cells decreased over time. Based on blood chimerism, the ratios of PBCs in the repaired meniscus were 20.5?±?2.3% at 1 week, 8.3?±?0.9% at 2 weeks, 4.4?±?0.9% at 4 weeks, 2.1?±?0.9% at 8 weeks, and 0.5?±?0.4% at 12 weeks, post-meniscectomy. Histologically, fibrochondrocytes were observed in the repaired meniscus of WT rats after 4 weeks, some of which were GFP-positive. The chondrogenic marker, type II collagen, was merged within the PBCs in the repaired tissue. However, type-II-collagen-positive cell ratio and metachromasia in the repaired meniscus were not equivalent in normal meniscal tissue. This indicated that PBCs were present within the repaired meniscus at an early phase, replacing the excised meniscal cells, suggesting PBCs contributed to meniscal healing. The tissue repair contribution by these cells decreased at later phases. Copyright © 2014 John Wiley & Sons, Ltd.
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Postnatal serum concentrations of endogenous free fatty acids in newborns admitted to the neonatal intensive care unit: effects on unbound bilirubin.
Ann. Clin. Biochem.
PUBLISHED: 03-06-2014
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Few studies have reported the characterization of postnatal serum concentrations of endogenous free fatty acids (FFAs) in high-risk newborns and their effects on unbound bilirubin (UB).
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Tandem keyhole foraminotomy in the treatment of cervical radiculopathy: retrospective review of 35 cases.
J Orthop Surg Res
PUBLISHED: 02-04-2014
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There has been no report regarding the results of two-level keyhole foraminotomy. The purpose of this study was to detail clinical outcomes following consecutive two-level cervical foraminotomy (tandem keyhole foraminotomy (TKF)) in patients with radiculopathy.
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Role of FDG-PET/CT for monitoring soft tissue tumors.
Oncol Lett
PUBLISHED: 01-23-2014
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The aim of the current study was to evaluate the limitations of 2-deoxy-2-F(18)-fluoro-D-glucose positron emission tomography combined with computed tomography (FDG-PET/CT) when monitoring soft tissue tumors. The diagnostic criteria of malignancy was defined as the tumor having a maximum standardized uptake value (SUVmax) ?2.0 and a maximum diameter ?5 cm as measured using FDG-PET/CT. One-hundred-and-thirteen patients, that were either included in the criteria or not, were compared. In addition, the values of SUVmax of the primary tumor and relapse in 12 patients were evaluated. The Kaplan-Meier analysis demonstrated that patients with tumors measuring ?5 cm size and ?2.0 SUVmax were associated with a worse survival rate. Among the 12 patients with relapse, statistical significances were detected in the tumor diameters, however, not in the SUVmax values. Thus, the criteria identified patients that were associated with a poor prognosis, and the SUVmax of distant metastases and local recurrences were identified to be significantly affected by tumor size.
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Combined medialis pedis and medial plantar fasciocutaneous flaps for coverage of soft tissue defects of multiple adjacent fingers.
Microsurgery
PUBLISHED: 01-16-2014
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Soft tissue defects of adjacent multiple fingers covered by a single large flap require secondary separation of the flap into each finger. Such covering obstructs independent motion of injured fingers until the single large flap is separated. This report describes the technique of combined medialis pedis and medial plantar fasciocutaneous flaps for reconstructing soft tissue defects of multiple adjacent fingers. Three male patients (age range, 18-33 years) underwent soft tissue reconstructions of multiple adjacent fingers with combined flaps. Injuries involved three adjacent palmar fingers, two adjacent palmar fingers, and two adjacent dorsal fingers. Average sizes of the combined flaps were 4.2 × 4.0 cm for the medialis pedis flap and 3.0 × 1.8 cm for the medial plantar fasciocutaneous flap. All flaps survived without vascular complications, and donor sites healed uneventfully. All patients experienced excellent recovery of range of motion for the reconstructed fingers. In conclusion, combined flaps may offer an alternative for coverage of soft tissue defects that involve multiple adjacent fingers.
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Spinopelvic alignment of diffuse idiopathic skeletal hyperostosis in lumbar spinal stenosis.
Eur Spine J
PUBLISHED: 01-11-2014
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The effect of diffuse idiopathic skeletal hyperostosis (DISH) on spinopelvic alignment in patients with lumbar spinal stenosis (LSS) remains unclear. The aim of this study was to investigate the association between DISH and sagittal spinopelvic alignment in patients undergoing surgery for LSS.
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A comparative study of clinicopathological features between simple bone cysts of the calcaneus and the long bone.
Foot Ankle Int
PUBLISHED: 01-09-2014
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The potential unrevealed clinicopathological differences between simple bone cysts situated in the calcaneus (calcaneal bone cysts) and those situated in long bones (long bone cysts) were investigated in the present study.
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Mobile-bearing prosthesis and intraoperative gap balancing are not predictors of superior knee flexion: a prospective randomized study.
Knee Surg Sports Traumatol Arthrosc
PUBLISHED: 01-06-2014
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Range of motion is a crucial measure of the outcome of total knee arthroplasty. Gap balancing technique and mobile-bearing prosthesis can improve postoperative range of motion. The purpose of this study was to determine the factors that are predictive of the postoperative range of motion.
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Gastric Invasive Micropapillary Carcinoma with Intestinal Phenotypes Harboring a TP53 R175H Mutation.
Case Rep Oncol
PUBLISHED: 01-01-2014
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We report a case of gastric invasive micropapillary carcinoma (IMPC) in an 86-year-old female patient. She was admitted to our hospital with a chief complaint of bloody emesis. Upper gastrointestinal endoscopy found a gastric adenocarcinoma at the antrum. The biopsy specimens showed moderately differentiated adenocarcinoma with invasive small tumor nests. Distal gastrectomy with systematic lymph node dissection demonstrated that the tumor had IMPC through a pathological examination. Despite the depth of tumor invasion (the submucosa), extensive lymph node metastases were observed. Anti-D2-40 immunostaining revealed numerous infiltrating tumor cell nests in the lymphatic vessels, which could explain subsequent multiple lymph node metastases. The adenocarcinoma showed intestinal phenotypes by several immunohistochemical studies. One of these antibodies (CD10) clearly demonstrated the inverted apical-basal (inside-out) pattern of IMPC, whereas it showed an ordinary pattern in intestinal metaplasia adjacent to the tumor. Furthermore, genetic analysis by direct sequencing revealed a point mutation in the exon 5 of TP53 in the tumor. The mutation presumably harbors a missense mutation from Arg to His at the codon 175 (R175H). R175H has been previously described as a 'gain-of-function' mutation with a high invasive or metastatic potential in several types of cancers. In summary, this is one of the first reported cases of gastric IMPC with intestinal phenotypes harboring a TP53 R175H mutation in the literature.
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Impact of atrial fibrillation termination site and termination mode in catheter ablation on arrhythmia recurrence.
Circ. J.
PUBLISHED: 11-02-2013
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Background:?Although atrial fibrillation (AF) termination has been reported as a predictor of clinical outcome after persistent AF (PsAF) ablation, the relationship between AF termination site and mode and clinical outcome has not been fully evaluated. Methods and Results:?A total of 135 patients (62±9 years) underwent their first ablation procedure for PsAF (76 longstanding PsAF). With an endpoint of AF termination, the ablation procedure was performed sequentially in the following order: pulmonary vein (PV) antrum isolation, and left atrial and right atrial substrate modification. AF termination was achieved in 69 patients (51%; 24 at the PV antrum, and 45 in the atrium; direct conversion to sinus rhythm in 21, and atrial tachycardia [AT] in 48). With a mean of 1.7±0.7 procedures/patient, 100 patients (74%) were free from atrial tachyarrhythmia (ATa) during a median of 15.0 months of follow-up. During the initial procedure, the AF termination site (atrium vs. PV antrum, hazard ratio [HR], 1.38; 95% confidence interval [CI]: 0.72-3.77; no termination vs. PV antrum, HR, 2.32; 95% CI: 1.26-6.30; P=0.023) and mode (AT vs. sinus rhythm, HR, 1.47; 95% CI: 0.77-4.01; no termination vs. sinus rhythm, HR, 2.38; 95% CI: 1.26-6.46; P=0.017) were independent predictors of ATa recurrence after the last ablation procedure. Conclusions:?The site and mode of AF termination during the index ablation procedure predict ATa recurrence following multiple catheter ablation procedures for PsAF.??(Circ J?2014; 78: 78-84).
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Application of the real-time Retinex image enhancement for endoscopic images.
Conf Proc IEEE Eng Med Biol Soc
PUBLISHED: 10-11-2013
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This paper presents a real-time image enhancement technique for gastric endoscopy, which is based on the variational approach of the Retinex theory. In order to efficiently reduce the computational cost required for image enhancement, processing layers and repeat counts of iterations are determined in accordance with software evaluation result, and as for processing architecture, the pipelining architecture can handle high resolution pictures in real-time. To show its potential, performance comparison between with and without the proposed image enhancement technique is shown using several video images obtained by endoscopy for different parts of digestive organ.
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Delayed progression of edema formation around a hematoma expressing high levels of VEGF and mmp-9 in a patient with traumatic brain injury: case report.
Neurol. Med. Chir. (Tokyo)
PUBLISHED: 09-27-2013
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The mechanisms accounting for the development of tissue damage following traumatic brain injury (TBI) have been studied for several decades. A variety of mediators, such as vascular endothelial growth factor (VEGF) and matrix metalloproteinase-9 (MMP-9), which play a crucial role in edema formation after TBI, have been identified. We experienced a case of brain edema that progressed continuously at least until 13 days after head injury. The brain edema occurred around the hemorrhage from an intracerebral contusion. The evacuated hematoma was investigated based on the inference that the unexpected expansion of edema was induced by the mediators within the hematoma itself. A 64-year-old woman was admitted to our hospital following a traffic injury. Left brain contusion was revealed by head computed tomography (CT) on admission. Three hours later, formation of an intracerebral hematoma became evident. Serial CT examination revealed that brain edema had developed progressively till 13 days after the injury. A hematoma removal operation was performed on Day 13. The hematoma was centrifuged and the supernatant was analyzed for the expression of VEGF and MMP-9. The values of both (4400 pg/ml and 920 ng/ml, respectively) were extremely high compared with values reported previously in serum and cerebrospinal fluid collected from patients with intracranial infection or injury. This case suggested that the delayed exacerbation of edema following traumatic intracranial hemorrhage was possibly induced by secretory factors such as VEGF and MMP-9 released from within and around the hematoma.
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Discrete prepotential as an indicator of successful ablation in patients with coronary cusp ventricular arrhythmia.
Circ Arrhythm Electrophysiol
PUBLISHED: 08-27-2013
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Although coronary cusp (CC) ventricular arrhythmia (VA) can be treated by catheter ablation, reliable indicators of successful ablation sites have not been fully identified.
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Six-year follow-up of catheter ablation in paroxysmal atrial fibrillation.
Circ. J.
PUBLISHED: 08-08-2013
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Although pulmonary vein (PV) antrum isolation is an established therapy for drug-resistant paroxysmal atrial fibrillation (PAF), long-term (>5 years) follow-up data are limited. This study investigated long-term clinical outcome of catheter ablation.
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Oxford phase 3 unicompartmental knee arthroplasty in Japan--clinical results in greater than one thousand cases over ten years.
J Arthroplasty
PUBLISHED: 07-26-2013
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There are few reports of the Oxford unicompartmental knee arthroplasty (UKA) survival rate in Asia. This study describes outcomes of 1279 Oxford UKAs for Japanese patients. The mean follow-up was 5.2 years. We divided patients into two groups based on preoperative indications (extended indications group and strict indications group). The Oxford knee score improved from 22.3 to 40.8 (P=0.041). The 10-year survival rate using revision was 95%. A total of 25 UKAs (2.0%) required revision. The most common reason was subsidence of tibial component. The 5-year cumulative survival rate of the strict indications group was significantly higher than that of the extended indications group (99.1% vs. 93.8%, P<0.001). When we followed inclusion criteria strictly, good clinical results were achieved in Asia.
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Clinical impact of adenosine triphosphate injection on arrhythmogenic superior vena cava in the context of atrial fibrillation ablation.
Circ Arrhythm Electrophysiol
PUBLISHED: 05-17-2013
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Superior vena cava (SVC) is an infrequent yet an important source of atrial fibrillation. The clinical impact of ATP injection on arrhythmogenic SVC has not been evaluated.
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Cancer stem-like cell marker CD44 promotes bone metastases by enhancing tumorigenicity, cell motility, and hyaluronan production.
Cancer Res.
PUBLISHED: 04-30-2013
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CD44, an adhesion molecule that binds to the extracellular matrix, primarily to hyaluronan (HA), has been implicated in cancer cell migration, invasion, and metastasis. CD44 has also recently been recognized as a marker for stem cells of several types of cancer. However, the roles of CD44 in the development of bone metastasis are unclear. Here, we addressed this issue by using bone metastatic cancer cell lines, in which CD44 was stably knocked down. Tumor sphere formation and cell migration and invasion were significantly inhibited by CD44 knockdown. Furthermore, the downregulation of CD44 markedly suppressed tumorigenicity and bone metastases in nude mice. Of note, the number of osteoclasts decreased in the bone metastases. Microarray analysis revealed that the expression of HA synthase 2 was downregulated in CD44-knockdown cells. The localization of HA in the bone metastatic tumors was also markedly reduced. We then examined the roles of CD44-HA interaction in bone metastasis using 4-methylumbelliferone (4-MU), an inhibitor of HA synthesis. 4-MU decreased tumor sphere and osteoclast-like cell formation in vitro. Moreover, 4-MU inhibited bone metastases in vivo with reduced number of osteoclasts. These results collectively suggest that CD44 expression in cancer cells promotes bone metastases by enhancing tumorigenicity, cell migration and invasion, and HA production. Our results also suggest the possible involvement of CD44-expressing cancer stem cells in the development of bone metastases through interaction with HA. CD44-HA interaction could be a potential target for therapeutic intervention for bone metastases.
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Four-component assembly in the crystalline state driven by amidinium-carboxylate salt bridge formation from an aqueous solution.
Org. Biomol. Chem.
PUBLISHED: 04-30-2013
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A series of diamidine dihydrochlorides was prepared utilizing a spacer unit to control the distance between the two amidinium groups. The introduction of two amidinium groups to the 1,8-position of each spacer unit (i.e., 9,10-dihydroanthracene, anthracene, biphenylene) can control the direction of formation of a self-assembled structure. The fine-tuning of the distances between the two amidinium groups in the spacer units can help control the stabilizing interactions of two carboxylic acid units (intermolecular attraction) after the four-component assembly (see ). Based on this concept, we succeeded in the formation of a four-component box-like assembled structure using amidinium-carboxylate salt bridge formation in the crystalline state from aqueous solutions.
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A case of dedifferentiated liposarcoma showing a biphasic pattern on 2-deoxy-2-f(18)-fluoro-d-glucose positron emission tomography/computed tomography.
Rare Tumors
PUBLISHED: 04-15-2013
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Integrated 2-deoxy-2-F(18)-fluoro-D-glucose positron emission tomography combined with computed tomography (FDG-PET/CT) has been used in the field of soft tissue sarcoma. We report an 81-year-old man with dedifferentiated liposarcoma in the left thigh, which was composed of well-differentiated liposarcoma and pleomorphic malignant fibrous histiocytoma. As well as other radiological modalities, FDG-PET was able to demonstrate a biphasic signal pattern composed of well-differentiated liposarcoma and dedifferentiated area, being consistent with the histological grade of malignancy.
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No difference in in vivo polyethylene wear particles between oxidized zirconium and cobalt-chromium femoral component in total knee arthroplasty.
Knee Surg Sports Traumatol Arthrosc
PUBLISHED: 04-11-2013
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Polyethylene wear particle generation is one of the most important factors affecting mid- to long-term results of total knee arthroplasties. Oxidized zirconium was introduced as a material for femoral components to reduce polyethylene wear generation. However, an in vivo advantage of oxidized zirconium on polyethylene wear particle generation is still controversial. The purpose of this study was to compare in vivo polyethylene wear particles between oxidized zirconium total knee prosthesis and conventional cobalt-chromium (Co-Cr) total knee prosthesis.
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Radiographic evaluation of postoperative bone regrowth after microscopic bilateral decompression via a unilateral approach for degenerative lumbar spondylolisthesis.
J Neurosurg Spine
PUBLISHED: 03-22-2013
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Minimally invasive decompressive surgery using a microscope or endoscope has been widely performed for the treatment of lumbar spinal canal stenosis (LSS). In this study the authors aimed to assess outcomes following microscopic bilateral decompression via a unilateral approach (MBDU) in terms of postoperative bone regrowth and preservation of the facet joints in patients with degenerative lumbar spondylolisthesis (DS) as compared with those in patients with LSS.
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Clinical characteristics of intraspinal facet cysts following microsurgical bilateral decompression via a unilateral approach for treatment of degenerative lumbar disease.
Eur Spine J
PUBLISHED: 03-20-2013
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Primary intraspinal facet cysts in the lumbar spine are uncommon, but it is unclear whether cyst incidence increases following decompression surgery and if these cysts negatively impact clinical outcome. We examined the prevalence, clinical characteristics, and the risk factors associated with intraspinal facet cysts after microsurgical bilateral decompression via a unilateral approach (MBDU).
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Clinical and Radiological Outcomes of Microscopic Partial Pediculectomy for Degenerative Lumbar Foraminal Stenosis.
Spine
PUBLISHED: 03-07-2013
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STRUCTURED ABSTRACT: Study Design. Retrospective cohort study.Objective. To investigate the clinical outcomes of microscopic partial pediculectomy for degenerative lumbar craniocaudal foraminal stenosis, risk factors for postsurgical scoliosis progression, and feasibility of postsurgical pedicle screw insertion.Summary of Background Data. Previous studies have evaluated surgical strategies for degenerative lumbar foraminal stenosis. Although less-invasive decompression surgery is an option for surgical treatment, postsurgical instability and salvaging fusion surgery remain as problems. No analysis has focused on the radiological progression and feasibility of pedicle screw setting after pediculectomy.Methods. Microscopic partial pediculectomy by our original method was performed as a first-choice surgical treatment for lumbar radiculopathy due to degenerative craniocaudal foraminal stenosis. This study included 50 consecutive patients followed up for a minimum of 2 years. Clinical outcomes were evaluated with Japanese Orthopedic Association (JOA) scores and a numerical rating scale (NRS). Radiological changes were obtained from standing X-rays. Foraminal height (FH) and the minimum pedicle diameter (MPD) were measured by reconstructed images on multidetector-row computed tomography.Results. The preoperative JOA score of 14.2 ± 4.2 significantly improved to 21.5 ± 6.2, and 60% of patients were satisfied. The NRS for lumbar back pain, leg pain, and leg numbness significantly improved. Nine patients (18%) showed lumbar Cobb angle progression of ?5° within 2 years, and the risk factor for scoliosis progression was surgery at L3-4 or L4-5 by multivariate logistic regression analysis. FH was enlarged from 5.4 mm preoperatively to 8.9 mm postoperatively. The postoperative MPD was 8.7 ± 1.6 (5.9-11.7) mm.Conclusion. Microscopic lumbar partial pediculectomy provided satisfactory clinical outcomes, but early postsurgical scoliosis progression was likely to occur in patients who underwent the surgery at L3-4 or L4-5. Even if a second surgical procedure is needed, pedicle screws can be set on the resected pedicle.
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Impact of low-dose dipyridamole injection on adenosine test after pulmonary vein isolation.
Pacing Clin Electrophysiol
PUBLISHED: 03-06-2013
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Dipyridamole increases the levels of extracellular adenosine. The study investigates the impact of low-dose intravenous dipyridamole on adenosine test after pulmonary vein (PV) isolation (PVI).
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Porous tantalum tibial component prevents periprosthetic loss of bone mineral density after total knee arthroplasty for five years-a matched cohort study.
J Arthroplasty
PUBLISHED: 02-27-2013
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In 21 knees receiving porous tantalum tibial component and 21 knees receiving a cemented cobalt-chromium tibial component, dual x-ray absorptiometry scans were performed for five years post-operatively. The postoperative decrease in the bone mineral density in the lateral aspect of the tibia was significantly less in knees with porous tantalum tibial components (11.6%) than in knees with cemented cobalt-chromium tibial components (29.6%) at five years (p < 0.05). No prosthetic migration or periprosthetic fracture was detected in either group. The present study is one of the studies with the longest follow-up period on bone mineral density after total knee arthroplasty. Porous tantalum tibial component has a favorable effect on the bone mineral density of the proximal tibia after total knee arthroplasty up to five years.
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Enhanced Bone-forming Activity of Side Population Cells in the Periodontal Ligament.
Cell Transplant
PUBLISHED: 02-05-2013
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Regeneration of alveolar bone is critical for the successful treatment of periodontal diseases. The periodontal ligament (PDL) has been widely investigated as a source of cells for the regeneration of periodontal tissues. In the present study, to develop an effective strategy for alveolar bone regeneration, we examined the osteogenic potential of side population (SP) cells which stem cell has been shown to be highly abundant in several kinds of tissues, in PDL cells. Isolated SP cells from the rat PDL exhibited superior ability to differentiate into osteoblastic cells compared with non-SP (NSP) and unsorted PDL cells in vitro. The mRNA expressions of osteoblast markers and bone morphogenetic protein (BMP) 2 were significantly up-regulated in SP cells and were further increased by osteogenic induction. To examine the bone-forming activity of SP cells in vivo, PDL SP cells isolated from green-fluorescence protein (GFP) transgenic rats were transplanted with hydroxyapatite (HA) disks into wild-type animals. SP cells exhibited high ability to induce the mineralized matrix compared with NSP and unsorted PDL cells. At 12 weeks after the implantation, some of the pores in HA disks with SP cells were filled with mineralized matrices, which were positive for bone matrix proteins such as osteopontin, bone sialoprotein, and osteocalcin. Furthermore, osteoblast- and osteocyte-like cells on and in the bone-like mineralized matrices were GFP-positive, suggesting that the matrices were directly formed by the transplanted cells. These results suggest that PDL SP cells possess enhanced osteogenic potential and could be a potential source for cell-based regenerative therapy for alveolar bone.
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Long-term complications of implantable defibrillator therapy in Brugada syndrome.
Am. J. Cardiol.
PUBLISHED: 01-16-2013
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Although the benefits of implantable cardioverter-defibrillator (ICD) therapy in patients with Brugada syndrome (BrS) at risk of sudden cardiac arrest are well established, these relatively young patients can encounter complications in the long term. The objective of the present study was to determine the incidence of device complications in patients with BrS during long-term follow-up. The prevalence of device-related complications and the clinical outcome were determined in 41 consecutive patients with BrS (age 48 ± 12 years; 38 men) who were treated with ICD implantation. During a median follow-up of 76 months (interquartile range 51 to 98), 15 patients (37%) experienced 21 adverse events, including 11 device-related complications in 8 (20%) and ?1 inappropriate shock in 10 (24%). Five patients (12%) received appropriate shocks for ventricular fibrillation. Excluding inappropriate shocks, 95.1%, 89.6%, 86.6%, and 73.3% of patients were free of device-related complications at 1, 3, 5, and 7 years, respectively. Also, 92.6%, 89.8%, 89.9%, and 86.1% were free of lethal arrhythmias at 1, 3, 5, and 7 years, respectively. Adverse effects related to ICD implantation are not uncommon, and their prevalence increases with the follow-up duration required for patients with BrS. In conclusion, because complications can be encountered even very late after device implantation, asymptomatic young patients should be carefully selected and educated about the long-term outcomes of ICD therapy.
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The role of internal fixation for long bone metastasis prior to impending fracture: an experimental model.
J Orthop Sci
PUBLISHED: 01-08-2013
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Patients with long bone metastasis have many therapeutic options, including surgery. However, the appropriate time for surgical intervention and the use of internal fixation prior to impending fracture remains controversial. The purpose of this study was to establish a long bone metastatic model with internal fixation, and to determine whether prophylactic internal fixation for long bone metastasis prior to impending fracture would affect bone destruction, tumor progression, and mortality.
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Acceleration of peripheral nerve regeneration using nerve conduits in combination with induced pluripotent stem cell technology and a basic fibroblast growth factor drug delivery system.
J Biomed Mater Res A
PUBLISHED: 01-07-2013
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Various modifications including addition of Schwann cells or incorporation of growth factors with bioabsorbable nerve conduits have been explored as options for peripheral nerve repair. However, no reports of nerve conduits containing both supportive cells and growth factors have been published as a regenerative therapy for peripheral nerves. In the present study, sciatic nerve gaps in mice were reconstructed in the following groups: nerve conduit alone (control group), nerve conduit coated with induced pluripotent stem cell (iPSc)-derived neurospheres (iPSc group), nerve conduit coated with iPSc-derived neurospheres and basic fibroblast growth factor (bFGF)-incorporated gelatin microspheres (iPSc + bFGF group), and autograft. The fastest functional recovery and the greatest axon regeneration occurred in the autograft group, followed in order by the iPSc + bFGF group, iPSc group, and control group until 12 weeks after reconstruction. Thus, peripheral nerve regeneration using nerve conduits and functional recovery in mice was accelerated by a combination of iPSc-derived neurospheres and a bFGF drug delivery system. The combination of all three fundamental methodologies, iPSc technology for supportive cells, bioabsorbable nerve conduits for scaffolds, and a bFGF drug delivery system for growth factors, was essential for peripheral nerve regenerative therapy. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2013.
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Clinical utility of adenosine-infusion test at a repeat atrial fibrillation ablation procedure.
Heart Rhythm
PUBLISHED: 01-05-2013
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The majority of patients with recurrence of arrhythmia after the initial atrial fibrillation (AF) ablation procedure have resumption of pulmonary vein (PV) conduction. Adenosine-infusion test after PV isolation identifies acute dormant PV conduction during the index procedure.
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Smoking counteracts the favorable effect of exercise training on endothelial function in patients with type 2 diabetes.
Jpn Clin Med
PUBLISHED: 01-01-2013
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Exercise training can improve endothelial function in patients with diabetes. We hypothesized that the favorable effect of exercise training on endothelial function in patients with diabetes is counteracted by cigarette smoking.
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Heparanase localization during palatogenesis in mice.
Biomed Res Int
PUBLISHED: 01-01-2013
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Palatogenesis is directed by epithelial-mesenchymal interactions and results partly from remodeling of the extracellular matrix (ECM) of the palatal shelves. Here, we assessed heparanase distribution in developing mouse palates. No heparanase was observed in the vertically oriented palatal shelves in early stages of palate formation. As palate formation progressed, the palatal shelves were reorganized and arranged horizontally above the tongue, and heparanase localized to the epithelial cells of these shelves. When the palatal bilateral shelves first made contact, the heparanase localized to epithelial cells at the tips of shelves. Later in fusing palatal shelves, the cells of the medial epithelial seam (MES) were labeled with intense heparanase signal. In contrast, the basement membrane heparan sulfate (HS) was scarcely observed in the palatal shelves in contact. Moreover, perlecan labeling was sparse in the basement membrane of the MES, on which laminin and type IV collagen were observed. Moreover, we assessed the distribution of matrix metalloproteinase- (MMP-) 9, MMP-2, and MMP-3 in developing mouse palates and these MMPs were observed in the MES. Our findings indicated that heparanase was important for palate formation because it mediated degradation of the ECM of palatal shelves. Heparanase may, in concert with other proteases, participate in the regression of the MES.
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Localization of SUMOylation factors and Osterix in odontoblast lineage cells during dentin formation and regeneration.
Histochem. Cell Biol.
PUBLISHED: 01-01-2013
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Small ubiquitin-related modifier (SUMO) conjugation (SUMOylation) is a post-translational modification involved in various cellular processes including the regulation of transcription factors. In this study, to analyze the involvement of SUMOylation in odontoblast differentiation, we examined the immunohistochemical localization of SUMO-1, SUMO-2/3, and Osterix during rat tooth development. At the bud and cap stages, localization of SUMOs and Osterix was hardly detected in the dental mesenchyme. At the bell stage, odontoblasts just beginning dentin matrix secretion and preodontoblasts near these odontoblasts showed intense immunoreactivity for these molecules. However, after the root-formation stage, these immunoreactivities in the odontoblasts decreased in intensity. Next, to examine whether the SUMOylation participates in dentin regeneration, we evaluated the distribution of SUMOs and Osterix in the dental pulp after cavity preparation. In the coronal pulp chamber of an untreated rat molar, odontoblasts and pulp cells showed no immunoreactivity. At 4 days after cavity preparation, positive cells for SUMOs and Osterix appeared on the surface of the dentin beneath the cavity. Odontoblast-like cells forming reparative dentin were immunopositive for SUMOs and Osterix at 1 week, whereas these immunoreactivities disappeared after 8 weeks. Additionally, we further analyzed the capacity of SUMO-1 to bind Osterix by performing an immunoprecipitation assay using C2C12 cells, and showed that Osterix could undergo SUMOylation. These results suggest that SUMOylation might regulate the transcriptional activity of Osterix in odontoblast lineage cells, and thus play important roles in odontoblast differentiation and regeneration.
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Partial laceration of the flexor tendon as an unusual cause of trigger finger.
J Plast Surg Hand Surg
PUBLISHED: 12-14-2011
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We present two cases of trigger finger caused by partial laceration of a flexor tendon. Both patients had preceding skin injury and required operative treatment with resection of the lacerated portion of the tendon and incision of the A1 pulley. We describe keys to the diagnosis of this type of lesion.
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Effect of right ventricular pacing site on QRS width.
Asian Cardiovasc Thorac Ann
PUBLISHED: 11-22-2011
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To assess the effect of various right ventricular pacing sites on QRS duration, we enrolled 50 patients (mean age, 64 ± 13 years; 36 men); 16 had bradycardia and 34 had tachycardia. The right ventricle was arbitrarily divided into 5 sections: high and low right ventricular outflow tract, mid septum, low septum, and apex. Right ventricular pacing was performed using an electrode catheter at each of the 5 sites. QRS duration was 162 ± 20 ms during high right ventricular outflow tract pacing, 143 ± 17 ms during low right ventricular outflow tract pacing, 151 ± 20 ms during mid-septal pacing, 163 ± 16 ms during low-septal pacing, and 167 ± 18 ms during apical pacing. Paced QRS duration was shorter during low right ventricular outflow tract and mid-septal pacing compared to apical pacing in 34 patients. There was a difference of 10 ms or less in the paced QRS duration between these pacing sites in the other 16 patients. QRS duration was shortest when the septum was paced in the right ventricle. However, QRS duration was similar during pacing in the septum and the apex in 32% of patients.
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[Infrastructure for new drug development to treat muscular dystrophy: current status of patient registration (remudy)].
Brain Nerve
PUBLISHED: 11-10-2011
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Clinical trials for new therapeutic strategies are now being planned for Duchenne and Becker muscular dystrophies (DMD/BMD); however, many challenges exist in the planning and conduction of a clinical trial for rare diseases. The epidemiological data, total number of patients, natural history, and clinical outcome measures are unclear. Adequate numbers of patients are needed to achieve significant results in clinical trials. As solutions to these problems, patient registries are an important infrastructure worldwide, especially in the case of rare diseases such as DMD/BMD. In Europe, TREAT-NMD, a clinical research network for neuromuscular disorders, developeda global database for dystrophinopathy patients. We developed a national registry of Japanese DMD/BMD patients in collaboration with TREAT-NMD. The database includes clinical and molecular genetic data as well as all required items for the TREAT-NMD global patient registry. As of July 2011, 750 patients were registered in the database. The purpose of this registry is the effective recruitment of eligible patients for clinical trials, and it may also provide timely information to individual patients about upcoming trials. This registry data also provides more detailed knowledge about natural history, epidemiology, and clinical care. In recent years, drug development has become dramatically globalized, and global clinical trials (GCTs) are being conducted in Japan. It is appropriate, particularly with regard to orphan diseases, to include Japanese patients in GCTs to increase evidence for evaluation, because such large-scale trials would be difficult to conduct solely within Japan. GCTs enable the synchronization of clinical drug development in Japan with that in Western countries, minimizing drug approval delays.
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Cardiac cycle-related volume change in unruptured cerebral aneurysms: a detailed volume quantification study using 4-dimensional CT angiography.
Stroke
PUBLISHED: 10-13-2011
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The hemodynamic factors of aneurysms were recently evaluated using computational fluid dynamics in a static vessel model in an effort to understand the mechanisms of initiation and rupture of aneurysms. However, few reports have evaluated the dynamic wall motion of aneurysms due to the cardiac cycle. The objective of this study was to quantify cardiac cycle-related volume changes in aneurysms using 4-dimensional CT angiography.
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Yeast thioredoxin-enriched extracts for mitigating the allergenicity of foods.
Biosci. Biotechnol. Biochem.
PUBLISHED: 10-07-2011
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Thioredoxin (TRX) catalyzes the reduction of disulfide bonds in proteins via the NADPH-dependent thioredoxin reductase system. Reducing the disulfide bonds of allergenic proteins in food by TRX lowers the allergenicity. We established in this study a method to prepare TRX-enriched extracts from the edible yeast, Saccharomyces cerevisiae, on a large and practical scale, with the objective of developing TRX-containing functional foods to mitigate food allergy. Treating with the yeast TRX-enriched extracts together with NADPH and yeast thioredoxin reductase enhanced the pepsin cleavage of ?-lactoglobulin and ovomucoid (OM). We also examined whether yeast TRX can mitigate the allergenicity of OM by conducting immediate allergy tests on guinea pigs. The treatment with TRX reduced the anaphylactic symptoms induced by OM in these tests. These results indicate that yeast TRX was beneficial against food allergy, raising the possibility that yeast TRX-enriched extracts can be applied to food materials for mitigating food allergy.
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Determination of ABO genotypes by real-time PCR using allele-specific primers.
Leg Med (Tokyo)
PUBLISHED: 10-04-2011
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ABO grouping of biological specimens is informative for identifying victims and narrowing down suspects. In Japan and elsewhere, ABO grouping as well as DNA profiling plays an essential role in crime investigations. In the present study, we developed a new method for ABO genotyping using allele-specific primers and real-time PCR. The method allows for the detection of three single nucleotide polymorphisms (SNPs) at nucleotide positions 261, 796, and 803 in the ABO gene and the determination of six major ABO genotypes. This method required less than 2 h for accurate ABO genotyping using 2.0 ng of DNA. This method could be applicable for rapid and simple screening of forensic samples.
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Prenatal molecular diagnosis of a severe type of L1 syndrome (X-linked hydrocephalus).
J Neurosurg Pediatr
PUBLISHED: 10-04-2011
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The aim of this study was to evaluate the feasibility of prenatal L1CAM gene testing for X-linked hydrocephalus (XLH).
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JoVE Visualize is a tool created to match the last 5 years of PubMed publications to methods in JoVE's video library.

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In developing our video relationships, we compare around 5 million PubMed articles to our library of over 4,500 methods videos. In some cases the language used in the PubMed abstracts makes matching that content to a JoVE video difficult. In other cases, there happens not to be any content in our video library that is relevant to the topic of a given abstract. In these cases, our algorithms are trying their best to display videos with relevant content, which can sometimes result in matched videos with only a slight relation.