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Find video protocols related to scientific articles indexed in Pubmed.
ABO Blood Group IgM Isoagglutinins Interact with Tumor-Associated O-Glycan Structures in Pancreatic Cancer.
Clin. Cancer Res.
PUBLISHED: 10-15-2014
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The ABO gene locus is associated with the risk of developing pancreatic ductal adenocarcinoma (PDAC) resulting in an increased incidence in individuals with non-O blood groups. Up to 90% of PDAC specimens display alterations in mucin type O-GalNAc glycosylation. Because aberrant O-GalNAc glycans (Tn and T antigen) are structurally related to blood group A and B glycans, we investigated the role of IgM isoagglutinins in PDAC.
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Elapid snake venom analyses show the specificity of the peptide composition at the level of genera Naja and Notechis.
Toxins (Basel)
PUBLISHED: 01-24-2014
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Elapid snake venom is a highly valuable, but till now mainly unexplored, source of pharmacologically important peptides. We analyzed the peptide fractions with molecular masses up to 10 kDa of two elapid snake venoms-that of the African cobra, N. m. mossambica (genus Naja), and the Peninsula tiger snake, N. scutatus, from Kangaroo Island (genus Notechis). A combination of chromatographic methods was used to isolate the peptides, which were characterized by combining complimentary mass spectrometric techniques. Comparative analysis of the peptide compositions of two venoms showed specificity at the genus level. Three-finger (3-F) cytotoxins, bradykinin-potentiating peptides (BPPs) and a bradykinin inhibitor were isolated from the Naja venom. 3-F neurotoxins, Kunitz/basic pancreatic trypsin inhibitor (BPTI)-type inhibitors and a natriuretic peptide were identified in the N. venom. The inhibiting activity of the peptides was confirmed in vitro with a selected array of proteases. Cytotoxin 1 (P01467) from the Naja venom might be involved in the disturbance of cellular processes by inhibiting the cell 20S-proteasome. A high degree of similarity between BPPs from elapid and viperid snake venoms was observed, suggesting that these molecules play a key role in snake venoms and also indicating that these peptides were recruited into the snake venom prior to the evolutionary divergence of the snakes.
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Proteome analysis of the effects of all-trans retinoic acid on human germ cell tumor cell lines.
J Proteomics
PUBLISHED: 06-27-2013
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We analysed the effects of all-trans retinoic acid (ATRA) on proliferation and changes in the global proteome of the nullipotent human embryonal carcinoma cell line 2102Ep and the pluripotent cell line NTERA2 cl.D1 (NT2). Differentially expressed proteins were assessed by 2D-PAGE and mass spectrometry, followed by verification and analysis of protein modifications of proteins of the retinoid pathway. We established a proteome map of the germ cell tumor (GCT) cell line NT2 showing neuronal differentiation under ATRA treatment for 7days. Using bioinformatic analyses, we identified functional groups of altered proteins and potentially involved pathways, of which changes to the organization of the cytoskeleton and anti-apoptotic effects were the most prominent. Changes observed in the expression of factors involved in the retinoid pathway under ATRA, namely an upregulation of CRBP and CRABP2, were also reflected in GCT tissues of different histologies, providing further insight into factors involved in the differentiation of these pluripotent tumors.
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Influence of cement film thickness on the retention of implant-retained crowns.
J Prosthodont
PUBLISHED: 02-11-2013
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The main goal of this study was to establish a new, high precision procedure to evaluate the influence of cement film thickness on the retention of cemented implant-retained crowns.
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Venom peptide analysis of Vipera ammodytes meridionalis (Viperinae) and Bothrops jararacussu (Crotalinae) demonstrates subfamily-specificity of the peptidome in the family Viperidae.
Mol Biosyst
PUBLISHED: 09-29-2011
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Snake venom peptidomes are valuable sources of pharmacologically active compounds. We analyzed the peptidic fractions (peptides with molecular masses < 10,000 Da) of venoms of Vipera ammodytes meridionalis (Viperinae), the most toxic snake in Europe, and Bothrops jararacussu (Crotalinae), an extremely poisonous snake of South America. Liquid chromatography/mass spectrometry (LC/MS), direct infusion electrospray mass spectrometry (ESI-MS) and matrix-assisted desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) were applied to characterize the peptides of both snake venoms. 32 bradykinin-potentiating peptides (BPPs) were identified in the Crotalinae venom and their sequences determined. 3 metalloproteinase inhibitors, 10 BPPs and a Kunitz-type inhibitor were observed in the Viperinae venom peptidome. Variability in the C-terminus of homologous BPPs was observed, which can influence the pharmacological effects. The data obtained so far show a subfamily specificity of the venom peptidome in the Viperidae family: BPPs are the major peptide component of the Crotalinae venom peptidome lacking Kunitz-type inhibitors (with one exception) while the Viperinae venom, in addition to BPPs, can contain peptides of the bovine pancreatic trypsin inhibitor family. We found indications for a post-translational phosphorylation of serine residues in Bothrops jararacussu venom BPP (S[combining low line]QGLPPGPPIP), which could be a regulatory mechanism in their interactions with ACE, and might influence the hypotensive effect. Homology between venom BPPs from Viperidae snakes and venom natriuretic peptide precursors from Elapidae snakes suggests a structural similarity between the respective peptides from the peptidomes of both snake families. The results demonstrate that the venoms of both snakes are rich sources of peptides influencing important physiological systems such as blood pressure regulation and hemostasis. The data can be used for pharmacological and medical applications.
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In vitro influence of ultrasonic stress, removal force preload and thermocycling on the retrievability of implant-retained crowns.
Clin Oral Implants Res
PUBLISHED: 07-04-2011
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The main goals of this in vitro study were to evaluate the influence of thermocycling, ultrasonic stress and the removal force preload on the retrievability of cemented implant crowns using a clinical removal device (Coronaflex) and evaluating the tensile strength using a universal testing machine (UTM).
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Assessment of lipopolysaccharide microleakage at conical implant-abutment connections.
Clin Oral Investig
PUBLISHED: 05-19-2011
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The aim of this in vitro study was to assess lipopolysaccharide microleakage at conical implant-abutment connections of two-piece dental implants in terms of the expression levels of genes involved in lipopolysaccharide-mediated proinflammatory cytokine production.
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Three-year clinical outcome of single implant-retained mandibular overdentures--results of preliminary prospective study.
J Dent
PUBLISHED: 04-05-2011
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The aim of this preliminary prospective study was to evaluate the clinical outcome, the oral health-related quality of life (OHRQoL), and the subjective chewing ability of patients with mandibular complete dentures retained by a single implant placed in the mandible midline.
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Tandem affinity depletion: a combination of affinity fractionation and immunoaffinity depletion allows the detection of low-abundance components in the complex proteomes of body fluids.
J. Proteome Res.
PUBLISHED: 10-21-2010
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Protein biomarker discovery in the low concentration range of human body fluids requires the enrichment of the proteins of interest. Here we report on a tandem affinity strategy: In the first step, we isolated a human plasma glyco-subproteome of healthy individuals by wheat germ agglutinin (WGA) lectin affinity chromatography. In the second step, the proteins of this subproteome were used to raise antibodies in llama (Lama glama). The heavy-chain fraction of the llama antibodies was used to deplete from the WGA lectin binding fraction all proteins normally found in human plasma. In this way, we selectively enriched the glycoprotein, CEA, a known cancer marker which had been spiked into normal plasma. As a proof of concept, we applied this method to the analysis of plasma sample from colon cancer patients. We could demonstrate the selective enrichment of CEA by a factor of 600-800.
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Discovery of a novel unfolded protein response phenotype of cancer stem/progenitor cells from the bone marrow of breast cancer patients.
J. Proteome Res.
PUBLISHED: 04-29-2010
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Metastases arise from disseminated tumor cells (DTC) that colonize secondary organs. However, DTC survival strategies to start metastatic outgrowth are unclear. The hostile (hypoxic, hypoglycemic) microenvironmental conditions of the bone marrow serve as an ideal model environment for investigation of DTC survival strategies under environmental stress. We investigated the breast cancer DTC cell line BC-M1 established from the bone marrow of a cancer patient by 2-D DIGE and MS analysis. We observed specific overexpression of the unfolded protein response (UPR) proteins Grp78, Grp94, and protein disulfide-isomerase in breast, lung, and prostate cancer DTC cell lines from the bone marrow. The UPR contributes to survival under adverse environmental conditions including chemotherapy. We show in cellular models that Grp78 expression of the UPR is regulated by tyrosine 1248 of ErbB-2. The breast cancer DTC cell lines shared stem/progenitor cell cancer phenotypes (CD44(high)/CD24(low)). Immunocytochemical staining of bone marrow samples from breast cancer patients confirmed in situ high expression of Grp78 and Grp94 in DTC of breast cancer patients, indicating the potential of both proteins as novel markers for DTC detection. Our results suggest the presence of a previously not recognized stress resistant DTC population that combines stem/progenitor attributes with an UPR phenotype.
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Changes in human mandibular bone morphology after heat application.
Ann. Anat.
PUBLISHED: 02-26-2010
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Intraosseous heat development is always a problem during bone surgery performed using rotary burs and ultrasound devices. However, only few data exist regarding the morphological effects of applied heat on bone surfaces.
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Patients and dentists perception of dental appearance.
Clin Oral Investig
PUBLISHED: 02-16-2010
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The purpose of this study was to compare the patients and dentists perception of dental appearance. Based on internationally accepted guidelines about dental esthetics, a questionnaire was developed to measure "dental appearance" (QDA). Eleven items defined a QDA sum score (0 =?"absolutely satisfied", 44 =?"absolutely dissatisfied"). The QDA was completed by 16 patients (eight women, eight men, mean age 63 ± 9 years) before and after a complete oral rehabilitation. Forty-two dentists evaluated the esthetics before and after rehabilitation on a visual analog scale (VAS, 0?=?"absolutely unesthetic", 100 =?"absolutely esthetic"). The patients quoting showed a significant esthetic improvement (QDA sum score) from 22 before treatment to 3.5 after treatment (P ? 0.001). Although most of the dentists judged an improvement in most of the patients dental appearances, no significant correlation could be found between patients and dentists judgment regarding dental appearance (r =?-0.13-0.53, P > 0.05). When evaluating the influence of age, gender, and experience on rating dental appearance, no significant differences (P > 0.05) could be found.
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Four-year clinical results of fixed dental prostheses with zirconia substructures (Cercon): end abutments vs. cantilever design.
Eur. J. Oral Sci.
PUBLISHED: 12-08-2009
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The purpose of this prospective study was to evaluate the clinical outcome of three- to four-unit posterior all-ceramic fixed dental prostheses (FDPs) made of yttria-stabilized tetragonal zirconia-polycrystal ceramic frameworks (CerconBase; Degudent). Fifty-eight restorations were placed in 48 patients. Twenty-four FDPs had an end abutment design (EAD) replacing 3 premolars and 21 molars. Thirty-four FDPs had a cantilever design (CD) replacing 11 premolars and 23 molars. The frameworks had a minimum proximal connector dimension of 3 x 3 mm. The fixed dental prostheses were cemented with glass-ionomer cement after air-abrading the inner crown surfaces. Three FDPs were defined as drop-outs. The mean observation period was 48 +/- 7 months for the EAD (21 patients/24 FDPs) and 50 +/- 14 months for the CD (25 patients/31 FDPs). The 4-yr survival rate, according to the Kaplan-Meier analyses, was 96% for the EAD and 92% for the CD. The technical complication rate was 13% for the EAD and 12% for the CD, and the biological complication rate was 21% for the EAD and 15% for the CD. For none of the analyses were significant differences found between both groups. After 4 yr the clinical outcome of three- to four-unit posterior FDPs with EAD and CD was promising.
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Two-dimensional differential gel electrophoresis of a cell line derived from a breast cancer micrometastasis revealed a stem/ progenitor cell protein profile.
J. Proteome Res.
PUBLISHED: 08-29-2009
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Dissemination of primary cancer cells to distant sites is an early event in breast cancer. These cells can invade the bone marrow, rest there, and many years later disseminated tumor cells (DTC) can grow out to form overt metastases. Epithelium specific cytokeratins are commonly used as marker proteins for sensitive detection of metastatic lesions. However, due to difficulties in the detection of DTC, the question arises if DTC necessarily have the same protein expression profile as advanced tumors. On that account, we analyzed the previously uncharacterized breast cancer DTC cell line BC-M1 by 2-D DIGE. Special protein concentration and purification protocols for 2-DE were developed which resulted in high recovery rates and increased display of alkaline proteins. A broad range reference map of metastasis relevant proteins was compiled including the cytokeratins 5, 7, 8, 17, 18, and 19 and several classes of cytoskeleton proteins involved in metastasis like ezrin, gelsolin, vinculin, or vimentin. BC-M1 shows the rare and highly metastatic vimentin/cytokeratin 5 positive and cytokeratin 8/18 negative breast cancer phenotype and expresses Her-2, which is also found in stem cells/progenitor cells of primary tumors. Supported by the detection of several other epithelium-derived proteins, the example BC-M1 indicates that the protein expression profile of DTC might be reminiscent of the expression profile of the early tumor, which differs from the advanced tumor. Hence, DTC from breast cancer patients bone marrow expressed cytokeratin 5, which further supports our hypothesis.
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Survival and complications of computer aided-designing and computer-aided manufacturing vs. conventionally fabricated implant-supported reconstructions: a systematic review.
Clin Oral Implants Res
PUBLISHED: 08-12-2009
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The aim of this systematic review was to assess the internationally published survival and complication rates of implant-supported computer-aided designing (CAD) and computer-aided manufacturing (CAM)-fabricated restorations and to compare them with those of conventionally fabricated implant-supported restorations.
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Performance of ultrasonic devices for bone surgery and associated intraosseous temperature development.
Int J Oral Maxillofac Implants
PUBLISHED: 07-10-2009
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The purpose of this study was to evaluate and to compare the bone-cutting performance and intraosseous temperature development of three modern ultrasonic devices for bone surgery (UDBS).
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Molecular leakage at implant-abutment connection--in vitro investigation of tightness of internal conical implant-abutment connections against endotoxin penetration.
Clin Oral Investig
PUBLISHED: 01-06-2009
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Microleakage has been discussed as a major contributing factor for inflammatory reactions at the implant-abutment connection. In previous studies, the tightness against corpuscular bodies (viable bacteria) has been successfully investigated under static and dynamic conditions. The aim of this study was to investigate the tightness against endotoxins of two implant systems (AstraTech and Ankylos) with conical internal connections under static conditions. The inner parts of eight implants of each system were inoculated with endotoxin. Implants were screwed together with the respective abutments and stored under isostatic conditions in a supernatant of pyrogen-free water for 168 h. Supernatant samples were taken after 5 min, 24 h, 72 h, and 168 h, and endotoxin contamination was determined by the amebocyte-lysate test. Only one implant in the AstraTech group showed no sign of endotoxin contamination after 168 h, while the other implants showed contamination after varying storage times, respectively. The implants in the Ankylos group showed endotoxin contamination after only 5 min of storage in the supernatant solution. The tested internal conical implant-abutment connections appear to be unable to prevent endotoxin leakage. In average, Astra implants showed a higher tightness than Ankylos implants.
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Increased intraosseous temperature caused by ultrasonic devices during bone surgery and the influences of working pressure and cooling irrigation.
Int J Oral Maxillofac Implants
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The purpose of this study was to investigate the increases in intraosseous temperature generated by a modern ultrasonic device for bone surgery (UDBS) and the influences of working pressure and cooling irrigation on this temperature.
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Influence of abutment height and thermocycling on retrievability of cemented implant-supported crowns.
Int J Oral Maxillofac Implants
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To evaluate the influence of abutment height and thermocycling on the retrievability of cemented implant crowns.
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Analysis of the intraimplant microflora of two-piece dental implants.
Clin Oral Investig
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Information about the spectrum of microorganisms in the intraimplant cavities of two-piece dental implants is scarce. The purpose of this study was to assess the intraimplant microflora of two-piece dental implants by conventional biochemical testing, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS), and 16 s rDNA gene sequencing.
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Surface contamination of dental implants assessed by gene expression analysis in a whole-blood in vitro assay: a preliminary study.
J. Clin. Periodontol.
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We aimed at evaluating pyrogen contamination of dental implants made of titanium and zirconia by using gene expression analysis in a whole-blood in vitro assay.
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Impact of vertical loading on the implant-bone interface.
Clin Oral Implants Res
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The main aim of this study was to evaluate the impact of vertical loading occurring during removal of cemented restorations on the implant-bone interface.
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Dental implants stimulate expression of Interleukin-8 and its receptor in human blood--an in vitro approach.
J. Biomed. Mater. Res. Part B Appl. Biomater.
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Interleukin (IL)-8 secreted from osteoblasts and peripheral blood monocytes increases in patients with aseptic hip-implant loss and in patients with mucositis after dental implant insertion. We explored in vitro the possibility of an IL-8-mediated inflammatory response as a consequence of contact between different dental implant surfaces and human blood. Titanium and zirconia implants were incubated in human blood. Nonstimulated blood served as negative, while blood stimulated with bacterial lipopolysaccharides (LPS) served as positive control. After depyrogenization, to examine the possible role of LPS, implants were again submerged in blood. Gene-expression of IL-8 and its receptor was measured by real-time quantitative polymerase chain reaction. In a receptor mediated, but LPS-independent manner, titanium implants led to a more pronounced increase in IL-8 gene expression when compared with zirconia implants. Depyrogenization resulted after 24 h in zirconia implants in decreased IL-8 gene expression. Altered IL-8 expression could indicate aseptic, at least LPS-independent implant loss, which may be an additional feature in the manifestation of peri-implantitis, possibly triggered by microscopically small implant-particles. Hence, opening a new field of investigations to further understand the possible mechanism underlying the manifestation of implant failure.
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Influence of the drill material and method of cooling on the development of intrabony temperature during preparation of the site of an implant.
Br J Oral Maxillofac Surg
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Our aim was to evaluate the intrabony friction heat produced by implant drills, using different drill materials and methods of cooling. Four pilot drills and 4 form drills were used. The following combinations of drill material and cooling supply were tested: steel and external cooling; steel and internal cooling; steel coated with zirconium nitride and external cooling; and zirconium oxide and external cooling. The handpiece that supported the drill was fixed in a lifting device. Specimens of bovine ribs were fixed below the handpiece, and the drill speed was set to 1200 rpm. The vertical force was adjusted to 1 kg for pilot drills and 0.5 kg for implant drills. Intrabony temperature during drilling was measured at depths of 4, 8, and 12 mm parallel to the drill, and the depth was limited to 13 mm. There were no significant differences in heat generation between the drill materials (p>.05), but the differences between groups with internal or external cooling supplies were significant (p?.05). The method of cooling affected the development of the intrabony temperature during preparation of the site of the implant, but the drill material seemed to play no particular role.
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JoVE Visualize is a tool created to match the last 5 years of PubMed publications to methods in JoVE's video library.

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We use abstracts found on PubMed and match them to JoVE videos to create a list of 10 to 30 related methods videos.

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In developing our video relationships, we compare around 5 million PubMed articles to our library of over 4,500 methods videos. In some cases the language used in the PubMed abstracts makes matching that content to a JoVE video difficult. In other cases, there happens not to be any content in our video library that is relevant to the topic of a given abstract. In these cases, our algorithms are trying their best to display videos with relevant content, which can sometimes result in matched videos with only a slight relation.