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Find video protocols related to scientific articles indexed in Pubmed.
Interstitial lung disorders in the indium workers of Korea: An update study for the relationship with biological exposure indices.
Am. J. Ind. Med.
PUBLISHED: 09-22-2014
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Korea is one of the highest indium-consuming countries worldwide. The present study aims to determine the relationship between interstitial lung disorders and indium exposure in Korea.
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Relationship Between Oxidative Stress and Bone Mass in Obesity and Effects of Berry Supplementation on Bone Remodeling in Obese Male Mice: An Exploratory Study.
J Med Food
PUBLISHED: 09-09-2014
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Abstract Berry consumption can prevent bone loss. However, the effects of different berries with distinct anthocyanin composition have not been thoroughly examined. The present study compared the effects of blueberry, blackberry, and black currant on bone health using a mouse model of diet-induced obesity. To investigate the effect of different berry supplements against a high-fat (HF) diet in vivo, 40 HF diet-induced obese (DIO) C57BL mice were assigned into four groups and fed a HF diet (35% w/w) with or without berry supplementation for 12 weeks (n=10). We measured adipose tissue mass (epididymal and retroperitoneal), plasma antioxidant, bone-related biomarkers, femur bone mineral density (BMD), and bone mineral content (proximal and distal). Adipose masses were negatively correlated with proximal BMD, but positively associated with plasma superoxide dismutase (SOD) concentrations (P<.001). Berry supplementation did not change the plasma ferric reducing antioxidant power, SOD, and insulin-like growth factor-1. However, the black currant group exhibited greater plasma alkaline phosphatase compared with the control group (P<.05). BMD in the distal epiphysis was significantly different between the blueberry and blackberry group (P<.05). However, berry supplementation did not affect bone mass compared with control. The present study demonstrates a negative relationship between fat mass and bone mass. In addition, our findings suggest that the anthocyanin composition of berries will affect bone turnover, warranting further research to investigate the underlying mechanisms.
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Impact of orange juice consumption on bone health of the U.S. population in the national health and nutrition examination survey 2003-2006.
J Med Food
PUBLISHED: 07-23-2014
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Orange juice (OJ) fortified with calcium (Ca) and vitamin D has turned OJ into a readily available source of these nutrients for children and adults. However, the impact of OJ consumption on Ca and vitamin D adequacy and bone health has not been documented. The aim of this study was the evaluation of the contribution of 100% OJ consumption to dietary and serum Ca and vitamin D status, and bone health parameters in the U.S. population aged 4 years and older (n=13,971) using the National Health and Nutrition Examination Survey (NHANES) 2003-2004 and 2005-2006. Food consumption data were coded to produce micronutrient intake values using the USDA Food and Nutrient Database for Dietary Studies 3.0. Serum concentrations of bone-related micronutrients and biomarkers, bone mineral density (BMD), and bone mineral content (BMC) were measured. Analysis of data was conducted using SAS software 9.2 and SUDAAN. OJ consumers showed higher intakes of bone-related micronutrients, compared with nonconsumers (P<.05). In addition, OJ consumers had higher serum Ca levels in adults (P<.01) and had a lower odds ratio for serum 25-hydroxyvitamin D3 <20?ng/mL in children (P<.05). OJ consumption was positively associated with femur BMD in children (P<.05) and with femur BMC in both children and adults (P<.05). In conclusion, OJ may be recommended as an effective dietary means of improving the status of Ca and vitamin D, acid-base balance, and of promoting bone health in children and adults.
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0159?The relationship between low level benzene exposure and blood cell counts in Korean workers.
Occup Environ Med
PUBLISHED: 07-15-2014
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Benzene is a well-known haematological toxin causing aplastic anaemia and leukaemia. Recent studies showed that low level benzene less than 1ppm disturbs the hematopoietic system. However, other studies did not show consistent results. The aim of the present study was to examine the relationship between low level benzene exposure and blood cell counts in Korean workers.
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0126?Workplace violence and depressive symptomatology among police officer.
Occup Environ Med
PUBLISHED: 07-15-2014
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The prevalence of depression in police officer is higher than in the general population. Police officers are often exposed to work place violence, long time work, shift work and other pcychosocial stressers. This study was conducted to evaluate the occupational factors associated with depressive symptomatology in police officer.
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Phase-pure FeSe(x) (x = 1, 2) nanoparticles with one- and two-photon luminescence.
J. Am. Chem. Soc.
PUBLISHED: 05-12-2014
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Iron chalcogenides hold considerable promise for energy conversion and biomedical applications. Realization of this promise has been hindered by the lack of control over the crystallinity and nanoscale organization of iron chalcogenide films. High-quality nanoparticles (NPs) from these semiconductors will afford further studies of photophysical processes in them. Phase-pure NPs from these semiconductors can also serve as building blocks for mesoscale iron chalcogenide assemblies. Herein we report a synthetic method for FeSe(x) (x = 1, 2) NPs with a diameter of ca. 30 nm that satisfy these needs. The high crystallinity of the individual NPs was confirmed by transmission electron microscopy (TEM) and energy-dispersive X-ray analysis. TEM tomography images suggest pucklike NP shapes that can be rationalized by bond relaxation at the NP edges, as demonstrated in large-scale atomic models. The prepared FeSe(x) NPs display strong photoluminescence with a quantum yield of 20%, which was previously unattainable for iron chalcogenides. Moreover, they also show strong off-resonant luminescence due to two-photon absorption, which should be valuable for biological applications.
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Berry anthocyanins suppress the expression and secretion of proinflammatory mediators in macrophages by inhibiting nuclear translocation of NF-?B independent of NRF2-mediated mechanism.
J. Nutr. Biochem.
PUBLISHED: 02-26-2014
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The objectives of this study were to compare the anti-inflammatory effects of anthocyanins from blueberry (BBA), blackberry (BKA), and blackcurrant (BCA) and to determine the relationship between their antioxidant capacity and anti-inflammatory effect in macrophages. Major anthocyanins in BBA, BKA and BCA were malvidin-3-glucoside (16%), cyanidin-3-glucoside (98%) and delphinidin-3-rutinoside (44%), respectively. BKA showed higher total antioxidant capacity than BBA and BCA. RAW 264.7 macrophages were incubated with 0-20 ?g/ml of BBA, BKA and BCA, and subsequently activated by lipopolysaccharide (LPS) to measure proinflammatory cytokine production. Interleukin 1? (IL-1?) messenger RNA (mRNA) levels were significantly decreased by all berry anthocyanins at 10 ?g/ml or higher. Tumor necrosis factor ? (TNF?) mRNA levels and secretion were also significantly decreased in LPS-treated macrophages. The levels of the repression were comparable for all berry anthocyanins. LPS-induced nuclear factor ?B (NF-?B) p65 translocation to the nucleus was markedly attenuated by all of the berry anthocyanins. In bone marrow-derived macrophages (BMMs) from nuclear factor E2-related factor 2 wild-type (Nrf2(+/+)) mice, BBA, BKA and BCA significantly decreased cellular reactive oxygen species (ROS) levels with a concomitant decrease in IL-1? mRNA levels upon LPS stimulation. However, in the BMM from Nrf2(-/-) mice, the anthocyanin fractions were able to significantly decrease IL-1? mRNA despite the fact that ROS levels were not significantly affected. In conclusion, BBA, BKA and BCA exert their anti-inflammatory effects in macrophages, at least in part, by inhibiting nuclear translocation of NF-?B independent of the NRF2-mediated pathways.
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The effect of scoliosis angle on center of gravity sway.
J Phys Ther Sci
PUBLISHED: 01-08-2014
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[Purpose] The purpose of this study was to verify the effects of idiopathic scoliosis on the human body by comparing the postural balance of adolescents with and without idiopathic scoliosis, to provide basic data for the optimal desirable growth and development of adolescents. [Subjects] The subjects were 128 adolescents diagnosed with scoliosis on X-ray by orthopedists. The subjects were divided into a 10 to 19 degree group, 20 to 29 degree group, and 30 degree and over group according to the degree of scoliosis. For comparison, 15 normal adolescents without orthopedic injury within the last 6 months were selected as a control group. [Methods] As measurement tools, DK2 525R (Dongkang Medical: Korea) was used to measure the Cobb angle and a multifunktional traininggeraete device (MFT, Germany) was used to measure balance. One-way variance of analysis was conducted in order to examine differences among the four groups in left and right balance, forward and backward balance, and overall postural balance, and when there were differences, they were compared in detail using Duncan's post-hoc test. [Results] The results of scoliosis angle and body mass index (BMI) showed significant differences between the normal group (NG) and the scoliosis groups (GI, G II, G III), but there were no significant differences among the scoliosis groups. The scoliosis groups showed a significantly lower BMI than that of the normal group. In addition, the results of the left/right and the front/rear balance abilities showed significant differences between the normal group and the scoliosis groups. Furthermore, the results of whole body balance ability were showed significant differences between the normal group and the scoliosis groups.
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Blood lead levels of Korean lead workers in 2003-2011.
Ann Occup Environ Med
PUBLISHED: 01-01-2014
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This study aimed to document the trend in blood lead levels in Korean lead workers from 2003 until 2011 and blood lead levels within each of the main industries.
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The effects of propionate and valerate on insulin responsiveness for glucose uptake in 3T3-L1 adipocytes and C2C12 myotubes via G protein-coupled receptor 41.
PLoS ONE
PUBLISHED: 01-01-2014
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Since insulin resistance can lead to hyperglycemia, improving glucose uptake into target tissues is critical for regulating blood glucose levels. Among the free fatty acid receptor (FFAR) family of G protein-coupled receptors, GPR41 is known to be the G?i/o-coupled receptor for short-chain fatty acids (SCFAs) such as propionic acid (C3) and valeric acid (C5). This study aimed to investigate the role of GPR41 in modulating basal and insulin-stimulated glucose uptake in insulin-sensitive cells including adipocytes and skeletal muscle cells. Expression of GPR41 mRNA and protein was increased with maximal expression at differentiation day 8 for 3T3-L1 adipocytes and day 6 for C2C12 myotubes. GPR41 protein was also expressed in adipose tissues and skeletal muscle. After analyzing dose-response relationship, 300 µM propionic acid or 500 µM valeric acid for 30 min incubation was used for the measurement of glucose uptake. Both propionic acid and valeric acid increased insulin-stimulated glucose uptake in 3T3-L1 adipocyte, which did not occur in cells transfected with siRNA for GPR41 (siGPR41). In C2C12 myotubes, these SCFAs increased basal glucose uptake, but did not potentiate insulin-stimulated glucose uptake, and siGPR41 treatment reduced valerate-stimulated basal glucose uptake. Therefore, these findings indicate that GPR41 plays a role in insulin responsiveness enhanced by both propionic and valeric acids on glucose uptake in 3T3-L1 adipocytes and C2C12 myotubes, and in valerate-induced increase in basal glucose uptake in C2C12 myotubes.
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Diets high in total antioxidant capacity improve risk biomarkers of cardiovascular disease: a 9-month observational study among overweight/obese postmenopausal women.
Eur J Nutr
PUBLISHED: 08-20-2013
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Previous studies have shown that total antioxidant capacity (TAC) of typical diets is associated with higher plasma TAC and antioxidant enzyme activities. At present, however, little is known for the association between dietary TAC and inflammatory biomarkers.
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Validation of an FFQ to assess antioxidant intake in overweight postmenopausal women.
Public Health Nutr
PUBLISHED: 06-26-2013
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To validate an FFQ to assess antioxidant intake in overweight postmenopausal women.
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Orange juice, a marker of diet quality, contributes to essential micronutrient and antioxidant intakes in the United States population.
J Nutr Educ Behav
PUBLISHED: 02-20-2013
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To evaluate the impact of 100% orange juice (OJ) on the healthy diet and micronutrient intakes of the United States population.
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5,8-Dimethoxy-2-Nonylamino-Naphthalene-1,4-Dione Inhibits Vascular Smooth Muscle Cell Proliferation by Blocking Autophosphorylation of PDGF-Receptor ?.
Korean J. Physiol. Pharmacol.
PUBLISHED: 01-24-2013
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As the abnormal proliferation of vascular smooth muscle cells (VSMCs) plays a critical role in the development of atherosclerosis and vascular restenosis, a candidate drug with antiproliferative properties is needed. We investigated the antiproliferative action and underlying mechanism of a newly synthesized naphthoquinone derivative, 5,8-dimethoxy-2-nonylamino-naphthalene-1,4-dione (2-nonylamino-DMNQ), using VSMCs treated with platelet-derived growth factor (PDGF). 2-Nonylamino-DMNQ inhibited proliferation and cell number of VSMCs induced by PDGF, but not epidermal growth factor (EGF), in a concentration-dependent manner without any cytotoxicity. This derivative suppressed PDGF-induced [(3)H]-thymidine incorporation, cell cycle progression from G0/G1 to S phase, and the phosphorylation of phosphor-retinoblastoma protein (pRb) as well as the expression of cyclin E/D, cyclin-dependent kinase (CDK) 2/4, and proliferating cell nuclear antigen (PCNA). Importantly, 2-nonylamino-DMNQ inhibited the phosphorylation of PDGF receptor?(PDGF-R?) enhanced by PDGF at Tyr(579), Tyr(716), Tyr(751), and Tyr(1021) residues. Subsequently, 2-nonylamino-DMNQ inhibited PDGF-induced phosphorylation of STAT3, ERK1/2, Akt, and PLC?1. Therefore, our results indicate that 2-nonylamino-DMNQ inhibits PDGF-induced VSMC proliferation by blocking PDGF-R? autophosphorylation, and subsequently PDGF-R?-mediated downstream signaling pathways.
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A case of esophageal intramural pseudodiverticulosis.
Gut Liver
PUBLISHED: 03-16-2011
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Esophageal intramural pseudodiverticulosis (EIP) is a rare benign disease that is characterized by multiple tiny flask-shaped outpouching lesions of the esophageal wall. The etiology is unknown, but the pathologic findings include dilatation of excretory ducts of submucosal glands. The predominant symptom is dysphagia, and esophageal stricture occurs frequently. Diseases such as diabetes mellitus, esophageal candidiasis, gastroesophageal reflux disease, and chronic alcoholism are often combined. Since most EIP cases are benign, the mainstream treatment is symptom relief by endoscopic dilatation or medical treatment of accompanied diseases. This report describes the case of a 68-year-old male patient who suffered from chest tightness for 2 months and was diagnosed with EIP. This symptom disappeared after 2 months of medical treatment, and the patient is now being regularly followed up.
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Neuroprotective effect of caffeoylquinic acids from Artemisia princeps Pampanini against oxidative stress-induced toxicity in PC-12 cells.
J. Food Sci.
PUBLISHED: 02-03-2011
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Phenolics in dry Artemisia princeps Pampanini, an herbal plant traditionally consumed as food ingredients in Korea was extracted, fractionated, and quantified as well as evaluated for its neuroprotection for PC-12 cells. Whole extract had 5,852 mg gallic acid equivalents/100 g of total phenolics and 6,274 mg and 9,698 mg vitamin C equivalents/100 g of antioxidant capacities assayed by DPPH and ABTS radicals, respectively. The fraction extracted with n-butanol had the highest levels of total phenolics and antioxidant capacity than the other fractions (n-hexane, chloroform, ethyl acetate, and water). Using a reversed-phase HPLC system, caffeoylquinic acid (CQA) and its derivatives such as 3-CQA, 4-CQA, 5-CQA, 1,5-diCQA, 3,4-diCQA, 3,5-diCQA, and 4,5-diCQA were isolated and quantified. The whole extract and its n-butanol fraction yielded 3,5-diCQA with the highest amount, which consisted of approximately 36.8% and 33.5%, respectively. The whole extract, the n-butanol fraction, and 3,5-diCQA showed neuroprotective effect on PC-12 cells under the insult of amyloid ß peptide in a dose-dependent manner. Treatments of the whole extract and the n-butanol fraction for PC-12 cells under oxidative stress increased approximately 1.6 and 2.4 times higher cell viability, compared with the control without treatments. For PC-12 cells treated with 3,5-diCQA, intracellular oxidative stress decreased by 51.3% and cell viability increased up to 2.8 times compared to the control with oxidative insult of amyloid ß peptide only. These results indicate that phenolics from A. princeps Pampanini alleviated the oxidative stress and enhanced the viability of PC-12 cells, suggesting that it may be applied as a dietary antineurodegenerative agent in functional foods.
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Fibrillar superstructure formation of hemoglobin A and its conductive, photodynamic and photovoltaic effects.
Acta Biomater
PUBLISHED: 02-01-2010
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The fabrication of biomaterials which serve as functional scaffolds exhibiting diversified effects has been valued. We report here a unique strategy to fibrillate hemoglobin A (HbA), which exhibits multiple photoelectrochemical properties, and a subsequent specific defibrillation procedure. A subtle structural rearrangement of the ?/?-subunits within the quaternary structure of HbA is responsible for the HbA fibril formation in the presence of 0.5% CHCl?. The narrow pH dependence of the suprastructure formation around pH 7.4 illustrates the highly sensitive nature of the structural alteration. The CHCl?-induced fibrils become disintegrated by ascorbic acid, indicating that the oxidation-reduction process of the iron within the heme moiety could be involved in stabilization of the fibrillar structures. The electron-transferring property of the iron allows the fibrils to exhibit not only their conductive behavior but also a photodynamic effect generating hydroxyl radicals in the presence of H(2)O(2) with light illumination. A photovoltaic effect is also demonstrated with the HbA fibrils, which generate an electric current on the fibril-coated microelectrode upon irradiation at 405nm. Taken together, the multiple effects of HbA fibrils and the selective fibrillation/defibrillation procedures could qualify the fibrils to be employed for various future applications in biotechnology, including bio-machine interfaces.
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Fumigant antitermitic activity of plant essential oils and components from Ajowan ( Trachyspermum ammi ), Allspice ( Pimenta dioica ), caraway ( Carum carvi ), dill ( Anethum graveolens ), Geranium ( Pelargonium graveolens ), and Litsea ( Litsea cubeba )
J. Agric. Food Chem.
PUBLISHED: 09-03-2009
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Plant essential oils from 26 plant species were tested for their insecticidal activities against the Japanese termite, Reticulitermes speratus Kolbe, using a fumigation bioassay. Responses varied with source, exposure time, and concentration. Among the essential oils tested, strong insecticidal activity was observed with the essential oils of ajowan ( Trachyspermum ammi ), allspice ( Pimenta dioica ), caraway ( Carum carvi ), dill ( Anethum graveolens ), geranium ( Pelargonium graveolens ), and litsea ( Litsea cubeba ). The composition of six essential oils was identified by using gas chromatography-mass spectrometry. The compounds thus identified were tested individually for their insecticidal activities against Japanese termites. Responses varied in a dose-dependent manner for each compound. Phenol compounds exhibited the strongest insecticidal activity among the test compounds; furthermore, alcohol and aldehyde groups were more toxic than hydrocarbons. The essential oils and compounds described herein merit further study as potential fumigants for termite control.
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Male-produced aggregation pheromone blend in Platypus koryoensis.
J. Agric. Food Chem.
PUBLISHED: 01-28-2009
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The ambrosia beetle, Platypus koryoensis , is a vector of Korean oak wilt disease, which causes massive mortality of oak trees (mainly Quercus mongolica ) in Korea. So that a semiochemical-based control method could be developed, its aggregation pheromone was investigated. Whole body extract and body part extracts of male and female P. koryoensis were analysized using gas chromatography-flame ionization detector (GC-FID) and gas chromatography-mass spectrometry (GC-MS). All samples of male extracts contained nerol, neral, geraniol, and geranial. Those compounds were detected from female whole body extract as minor constituents and not detected from any female body part extracts. In addition to those compounds, citronellol was detected from the extract of boring dust produced by an unmated male. However, none of the five compounds were detected from the extract of boring dust produced by mated males and females or in artificial sawdust obtained from a beetle-infected Q. mongolica log. Male and female antennae of P. koryoensis responded to all five compounds in an electroantennography test. The blend of five components was tested in the field and attractive for male and female P. koryoensis. This result suggested that the blend of citronellol, nerol, neral, geraniol, and geranial served as an aggregation pheromone to P. koryoensis.
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Validation of an FFQ to assess short-term antioxidant intake against 30 d food records and plasma biomarkers.
Public Health Nutr
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OBJECTIVE: To validate a brief FFQ developed for capturing short-term antioxidant intake in a sample of US college students. DESIGN: A seventy-four-item antioxidant FFQ was developed based on major antioxidant sources in the American diet. The FFQ was validated against 30 d food records (FR) and plasma antioxidant concentrations. The reliability of the FFQ was evaluated by two FFQ administered at a 1-month interval. Settings University of Connecticut, CT, USA. SUBJECTS: Sixty healthy college students. RESULTS: Estimates of dietary antioxidants from the FFQ were moderately to highly correlated with those estimated from the 30 d FR (r = 0·29-0·80; P < 0·05) except for ?-tocopherol and ?-cryptoxanthin. Total antioxidant capacity from diet only or from diet and supplements estimated by the 30 d FR and FFQ were highly correlated (r = 0·67 and 0·71, respectively; P < 0·0001). The FFQ categorized 91 % of participants into the same or adjacent tertiles of antioxidant intake as the 30 d FR. Most dietary carotenoids estimated from the FFQ were correlated with plasma levels (P < 0·05). Correlation coefficients for test-retest reliability ranged from 0·39 to 0·86. More than 94 % of the participants were classified in the same or adjacent tertiles between the two administrations of the FFQ. CONCLUSIONS: The brief FFQ demonstrated reasonable validity for capturing a comprehensive antioxidant intake profile. This FFQ is applicable in epidemiological or clinical studies to capture short-term antioxidant intake or to simply document the variations of antioxidant intake in intervention trials. Cross-validation studies are warranted in other target populations.
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Would complex decongestive therapy reveal long term effect and lymphoscintigraphy predict the outcome of lower-limb lymphedema related to gynecologic cancer treatment?
Gynecol. Oncol.
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The aims of this study were to investigate the long-term effect of complex decongestive therapy (CDT) on lymphedema volume reduction, especially considering the proximal and distal parts of the leg, and to evaluate the utility of pre-therapy lymphoscintigraphy in predicting the response to CDT in patients with lower-limb lymphedema after surgery for gynecologic cancer.
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Dietary total antioxidant capacity is associated with diet and plasma antioxidant status in healthy young adults.
J Acad Nutr Diet
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Dietary total antioxidant capacity (TAC), based on the cumulative antioxidant activities of all the antioxidants present in food, has been shown to be inversely associated with risks of chronic diseases. However, dietary TAC has not been validated for its relevance in a healthy young population or for reliability and predictability for antioxidant status. Our study aimed to validate TAC as a tool in assessing antioxidant intake and to investigate whether dietary TAC predicts plasma antioxidant status in a healthy young population. Sixty healthy, nonsmoking college students at the University of Connecticut ages 18 to 25 years were recruited. Thirty-day food records and two 12-hour fasting blood samples were collected for dietary and plasma antioxidant assessments. After adjustment for total energy intake, TAC from diet and supplement was positively correlated with intakes of carotenoids (P<0.01), beta carotene (P<0.05), ?-cryptoxanthin (P<0.05), flavonoids (P<0.0001), isoflavones (P<0.01), flavan-3-ols (P<0.01), flavones (P<0.05), and flavonols (P<0.0001). Dietary TAC was an independent predictor of plasma TAC determined by vitamin C equivalent antioxidant capacity (P<0.01) and by ferric-reducing ability of plasma (P<0.0001), plasma glutathione peroxidase (P<0.01), red blood cell glutathione peroxidase (P<0.05), ?-tocopherol (P<0.05), and lutein (P<0.05). Results were similar for TAC from diet sources only. The findings suggest that dietary TAC is a good predictor of dietary and plasma antioxidant status in this sample of young adult men and women.
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Outcomes of wrist arthroplasty using a free vascularized fibular head graft for Enneking stage II giant cell tumors of the distal radius.
Microsurgery
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The purpose of this study was to report the clinical outcomes of wrist arthroplasty with a free vascularized fibular head graft (FVFHG) and to highlight some considerations that may affect outcomes. FVFHG was performed on 12 patients with giant cell tumors of the distal radius between April 1984 and July 2005. The mean age of patients was 33 years. All 12 patients were classified as Enneking stage 2. Outcomes were evaluated with radiographic and functional assessments, including the scale of Enneking. The mean follow-up period was 6.26 years. Bone union was achieved in all patients at a mean of 15.7 weeks after surgery. Skin grafting was performed at the recipient site in 5 patients and had good skin healing. Subluxation in the wrist joint was observed in 5 patients and was related to the length of the transplanted fibula. The 5 patients with subluxation experienced considerable osteoarthritic change. The mean arc of flexion-extension and rotation of the wrist joint was 73.1° and 102.9°, respectively. The mean grip strength was 57.25% of the contralateral side. The mean functional score was 26.4 points. Wrist arthroplasty with a FVFHG is a useful option to treat Enneking stage 2 giant cell tumors of the distal radius. We believe that wrist instability is not determined by the choice of laterality of the fibula, which can be minimized by transplanting a short fibula with the anterior tibial artery as a donor artery. The recipient sites can be successfully resurfaced by skin grafting.
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Estimated flavonoid intake of the elderly in the United States and around the world.
J Nutr Gerontol Geriatr
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The aging population has been growing fast in the United States and worldwide. The morbidity of age-related chronic degenerative diseases has also been increasing in parallel. Numerous studies have reported that consumption of flavonoid-rich fruits and vegetables is inversely associated with such chronic diseases as Alzheimers disease, age-related macular degeneration, cardiovascular disease, and osteoporosis. In establishing flavonoids as one of the contributors to the protective effects, the very first step is to estimate flavonoid intake from various dietary sources. Estimation of flavonoid intake from dietary sources has been feasible since 2003 when the U.S. Department of Agriculture (USDA) released the database for the flavonoid content of selected foods. Since then, several articles have been published in which flavonoid intake in various subpopulation groups was estimated from relatively large, current databases of flavonoid concentration data. However, information is still limited on the intake by seniors in the United States and worldwide. This review summarizes the most current estimates of flavonoid intake by seniors in the United States and elsewhere.
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?-Casein-based hierarchical suprastructures and their use for selective temporal and spatial control over neuronal differentiation.
Biomacromolecules
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Functions are diversified by producing hierarchical structures from a single raw material. Biologically compatible milk protein of ?-casein has been employed to fabricate higher-order suprastructures. In the presence of dithiothreitol and heat treatment, ?-casein transforms into amyloid fibrils with distinctive morphology attributable to mechanism-based fibrillar polymorphism. As the fibrils elongate to yield high aspect ratio during high-temperature incubation, the resulting fibrils laterally associate into the liquid crystalline state by forming a two-dimensional fibrillar array. Following a desalting process, the fibrillar arrays turn into a three-dimensional matrix of hydrogel that could be selectively disintegrated by subsequent salt treatment. The hydrogel was demonstrated to be a matrix capable of exhibiting controlled release of bioactive substances like retinoic acid, which led to temporal and spatial control over the differentiation of neuronal cells. Therefore, the hierarchical suprastructure formation derived from the single protein of ?-casein producing one-dimensional protein nanofibrils, a two-dimensional liquid crystalline state and a three-dimensional hydrogel could be widely appreciated in various areas of nanobiotechnology including drug delivery and tissue engineering.
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Effect of eriodictyol on glucose uptake and insulin resistance in vitro.
J. Agric. Food Chem.
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Eriodictyol [2-(3,4-dihydroxyphenyl)-5,7-dihydroxy-2,3-dihydrochromen-4-one] is a flavonoid with anti-inflammatory and antioxidant activities. Because inflammation and oxidative stress play critical roles in the pathogenesis of diabetes mellitus, the present study was designed to explore whether eriodictyol has therapeutic potential for the treatment of type 2 diabetes. The results show that eriodictyol increased insulin-stimulated glucose uptake in both human hepatocellular liver carcinoma cells (HepG2) and differentiated 3T3-L1 adipocytes under high-glucose conditions. Eriodictyol also up-regulated the mRNA expression of peroxisome proliferator-activated receptor ?2 (PPAR?2) and adipocyte-specific fatty acid-binding protein (aP2) as well as the protein levels of PPAR?2 in differentiated 3T3-L1 adipocytes. Furthermore, it reactivated Akt in HepG2 cells with high-glucose-induced insulin resistance. This response was strongly inhibited by pretreatment with the phosphatidylinositol 3-kinase (PI3K) inhibitor LY294002, indicating that eriodictyol increased Akt phosphorylation by activating the PI3K/Akt pathway. These results imply that eriodictyol can increase glucose uptake and improve insulin resistance, suggesting that it may possess antidiabetic properties.
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Plasma total antioxidant capacity is associated with dietary intake and plasma level of antioxidants in postmenopausal women.
J. Nutr. Biochem.
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Increased plasma total antioxidant capacity (TAC) has been associated with a high consumption of fruits and vegetables. However, limited information is available on whether plasma TAC reflects the dietary intake of antioxidants and the levels of individual antioxidants in plasma. By using three different assays, the study aimed to determine if plasma TAC can effectively predict dietary intake of antioxidants and plasma antioxidant status. Forty overweight and apparently healthy postmenopausal women were recruited. Seven-day food records and 12-h fasting blood samples were collected for dietary and plasma antioxidant assessments. Plasma TAC was determined by vitamin C equivalent antioxidant capacity (VCEAC), ferric-reducing ability of plasma (FRAP) and oxygen radical absorbance capacity (ORAC) assays. TAC values determined by VCEAC were highly correlated with FRAP (r=0.79, P<.01) and moderately correlated with ORAC (r=0.34, P<.05). Pearson correlation analyses showed that plasma TAC values by VCEAC and ORAC had positive correlation with plasma uric acid (r=0.56 for VCEAC; r=0.49 for ORAC) and total phenolics (r=0.63 for VCEAC; r=0.36 for ORAC). However, TAC measured by FRAP was correlated only with uric acid (r=0.69). After multivariate adjustment, plasma TAC determined by VCEAC was positively associated with dietary intakes of ?-tocopherol (P<.001), ?-carotene (P<.05), anthocyanidins (P<.05), flavones (P<.05), proanthocyanidins (P<.01) and TAC (P<.05), as well as with plasma total phenolics (P<.05), ?-tocopherol (P<.001), ?-cryptoxanthin (P<.05) and uric acid (P<.05). The findings indicate that plasma TAC measured by VCEAC reflects both dietary and plasma antioxidants and represents more closely the plasma antioxidant levels than ORAC and FRAP.
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Impact of orange juice consumption on macronutrient and energy intakes and body composition in the US population.
Public Health Nutr
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The present study evaluated the contribution of 100 % orange juice (OJ) consumption to the intakes of macronutrients and energy and its impact on body composition.
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What is Visualize?

JoVE Visualize is a tool created to match the last 5 years of PubMed publications to methods in JoVE's video library.

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We use abstracts found on PubMed and match them to JoVE videos to create a list of 10 to 30 related methods videos.

Video X seems to be unrelated to Abstract Y...

In developing our video relationships, we compare around 5 million PubMed articles to our library of over 4,500 methods videos. In some cases the language used in the PubMed abstracts makes matching that content to a JoVE video difficult. In other cases, there happens not to be any content in our video library that is relevant to the topic of a given abstract. In these cases, our algorithms are trying their best to display videos with relevant content, which can sometimes result in matched videos with only a slight relation.