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Find video protocols related to scientific articles indexed in Pubmed.
Chinese Herbal Medicine for Obesity: A Randomized, Double-Blinded, Multicenter, Prospective Trial.
Am. J. Chin. Med.
PUBLISHED: 11-20-2014
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Obesity is a serious medical problem worldwide. As a holistic therapy, traditional Chinese medicine (TCM) may have a potential in obesity management. In this controlled trial, we evaluated the safety and effectiveness of Xin-Ju-Xiao-Gao-Fang (XJXGF), a TCM herbal formulation, in 140 obese subjects over a 24-week period. The XJXGF formula mainly consists of rhubarb, coptis, semen cassia, and citrus aurantium. Subjects with body mass index (BMI) 28-40 kg/m(2) were recruited at 5 centers in China. We assessed the changes in subjects' body weight, its related parameters, and the reduction of insulin resistance (IR) after administration of XJXGF formula or low-dose XJXGF (10% of the XJXGF formula, as control). After 24-week treatment, among participants in the XJXGF formula group and low-dose XJXGF group, the mean ± SE changes in the body weight were -3.58±0.48 and -1.91±0.38 kg, respectively (p < 0.01). The changes in the IR-index of two groups were -2.65±1.04 and -1.58±1.3, respectively (p < 0.05). There were no serious adverse events reported during the 24-week trial. Participants reported 7 minor adverse events, 4 in the XJXGF formula group and 3 in the low-dose XJXGF group (p = 0.578). Future studies are needed to investigate the clinical utility of this TCM formulation in the treatment of obese subjects.
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Salvianolic acids T and U: a pair of atropisomeric trimeric caffeic acids derivatives from root of Salvia miltiorrhiza.
Fitoterapia
PUBLISHED: 08-27-2014
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Two new trimeric caffeic acids, named salvianolic acids T and U (1 and 2), were isolated from the underground part of Salvia miltiorrhiza. Their structures, consisting of three caffeic acid units, were determined based on extensive 1D- and 2D-spectroscopic analyses and electronic circular dichroism (ECD) calculations.
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Objective assessment of persistent rhinitis in Chinese and its relationship with serum indicators.
Eur Arch Otorhinolaryngol
PUBLISHED: 08-20-2014
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Persistent rhinitis (PR) is a chronic disease that affects millions of people. However, it lacks of a useful method, which can indicate the actual severity of the inflammation in PR patients. This study was designed to seek an examination which could reflect the actual severity of PR disease. The serum Phadiatop test, ECP level, four-phase rhinomanometry, and acoustic rhinometry were assessed in 91 adult patients with PR and 10 healthy controls. The serum total IgE was determined in some of the patients and all of the controls. The patients were divided into four groups: ARWO, ARWTO, NARWO and NARWTO. 40 % (22/55) of AR and 33.3 % (13/36) of NAR patients never complained of persistent nasal obstruction. Serum ECP levels were increased in the ARWO group. Serum total IgE was significantly elevated in the AR groups. MCA1-Min and MCA1-T were significantly reduced in the ARWO, ARWTO, and NARWO groups. NV6-Min and NV6-T were decreased in all PR groups, but only some of these differences were significant. In the ARWO group, MCA2-Min (r = -0.252), MCA2-T (r = -0.377), NV6-Min (r = -0.32), and NV6-T (r = -0.311) had significant relationships with serum ECP. We recommend acoustic rhinometry as a useful routine tool for the diagnosis of PR, even among patients without persistent subjective nasal obstruction. This technique might reveal the actual status of nasal congestion. An elevated serum ECP level might indicate severe AR and is negatively correlated with the results of acoustic rhinometry.
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[Two cases of African imported Plasmodium malariae malaria].
Zhongguo Xue Xi Chong Bing Fang Zhi Za Zhi
PUBLISHED: 07-24-2014
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The process of diagnosis, epidemiological survey and treatment of two cases of African imported quartan malaria is reported in this paper.
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Mutation breeding of Saccharomyces cerevisiae with lower methanol content and the effects of pectinase, cellulase and glycine in sugar cane spirits.
J. Sci. Food Agric.
PUBLISHED: 07-09-2014
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To decrease the methanol content of the sugar cane sprits, mutagenesis of ultraviolet (UV) coupled with diethyl sulfate (DES) was used to generate a mutant of Saccharomyces cerevisiae with lower methanol content. Meanwhile, the effects of the additions of pectinase, cellulase and glycine on the production of methanol in sugar cane spirits were evaluated.
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Apigenin suppresses GLUT-1 and p-AKT expression to enhance the chemosensitivity to cisplatin of laryngeal carcinoma Hep-2 cells: an in vitro study.
Int J Clin Exp Pathol
PUBLISHED: 06-15-2014
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Glucose transporter-1 (GLUT-1) and PI3K/Akt are known to be closely involved in resistance to chemotherapy. Co-targeted therapy reducing GLUT-1 expression and PI3K/Akt pathway activity may overcome the chemoresistance of human cancers. Apigenin may inhibit the expression of GLUT-1 and the PI3K/Akt pathway. We hypothesized that over-expression of GLUT-1 and p-Akt was associated with the resistance to cisplatin of laryngeal carcinoma Hep-2 cells. We explored whether apigenin inhibited GLUT-1 and p-Akt, resulting in sensitization of laryngeal carcinoma Hep-2 cells to cisplatin. Real-time RT-PCR and Western blotting confirmed the presence of GLUT-1 mRNA, and GLUT-1 and p-Akt proteins in Hep-2 cells. We found that resistance or insensitivity of Hep-2 cells to cisplatin might be associated with such expression. Apigenin markedly enhanced the cisplatin-induced suppression of Hep-2 cell growth. This effect was concentration- and time-dependent. Thus apigenin may significantly reduce the levels of GLUT-1 mRNA, and GLUT-1 and p-Akt proteins, in cisplatin-treated Hep-2 cells, in a concentration- and time-dependent manner. To conclude, overexpression of GLUT-1 mRNA may be associated with the resistance to cisplatin of laryngeal carcinoma Hep-2 cells. Apigenin may enhance the sensitivity to cisplatin of laryngeal carcinoma cells via inhibition of GLUT-1 and p-Akt expression.
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Submucosal small-cell neuroendocrine carcinoma of the larynx detected using (18)F-fluorodeoxyglucose positron emission tomography/computed tomography: A case report and review of the literature.
Oncol Lett
PUBLISHED: 06-12-2014
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A 67-year-old male presented with a metastatic carcinoma in the right side of the neck from an unknown primary site. (18)F-fluorodeoxyglucose (FDG) positron emission tomography/computed tomography showed increased (18)F-FDG uptake in the right larynx and right neck lymph nodes. A smooth lesion was identified in the submucosa of the right supraglottic region via a suspension laryngoscopy under general anaesthesia. A biopsy was performed and a frozen section revealed a small-cell (SC) carcinoma. A total laryngectomy and bilateral neck dissection were performed simultaneously, and the pathological results demonstrated a SC neuroendocrine carcinoma. The patient received chemo-radiotherapy postoperatively, however, succumbed due to distant metastasis one year following surgery.
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Clinical features and hypoxic marker expression of primary sinonasal and laryngeal small-cell neuroendocrine carcinoma: a small case series.
World J Surg Oncol
PUBLISHED: 06-12-2014
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Small-cell neuroendocrine carcinoma (SCNEC) of the head and neck is rare. The prognosis of SCNEC in the nasal cavity and larynx is poor. The aim of this study was to investigate the clinicopathological features of nasal and laryngeal SCNEC and to determine the expression of HIF-1?, GLUT-1, PI3K, and p-Akt in SCNEC.
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Reduction of methanol in brewed wine by the use of atmospheric and room-temperature plasma method and the combination optimization of malt with different adjuncts.
J. Food Sci.
PUBLISHED: 05-17-2014
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Methanol, often generated in brewed wine, is highly toxic for human health. To decrease the methanol content of the brewed wine, atmospheric and room-temperature plasma (ARTP) was used as a new mutagenesis tool to generate a mutant of Saccharomy cescerevisiae with lower methanol content. Headspace gas chromatography was used to determine the identity and concentration of methanol with butyl acetate as internal standard in brewed wine. With 47.4% higher and 26.3% positive mutation rates were obtained, the ARTP jet exhibited a strong effect on mutation breeding of S. cerevisiae. The mutant S. cerevisiae S12 exhibited the lowest methanol content, which was decreased by 72.54% compared with that of the wild-type strain. Subsequently, the mutant S. cerevisiae S12 was used to ferment different combinations of malt and adjuncts for lower methanol content and higher alcoholic content. It was shown that the culture 6#, which was 60% malt, 20% wheat, and 20% corn, was the best combinations of malt and adjuncts, with the lowest methanol content (104.8 mg/L), and a relatively higher alcoholic content (15.3%, v/v). The optimal malt-adjunct culture 6#, treated with the glucoamylase dose of 0.04 U/mg of grain released the highest reducing sugars (201.6 mg/mL). It was indicated that the variation in reducing sugars among the combinations of malt and different adjuncts could be due to the dose of exogenous enzymes.
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The utility of repeated computed tomography to track a foreign body penetrating the esophagus to the level of the thyroid gland.
Oral Radiol
PUBLISHED: 05-13-2014
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Foreign body (FB) ingestion is a common problem in otolaryngology. One uncommon complication of FB ingestion is penetration to the level of the thyroid gland. To our knowledge, only 21 such cases have been reported in the literature. Here, we report a case of an esophageal FB penetrating to the level of the right thyroid gland.
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Post-harvest induced production of salvianolic acids and significant promotion of antioxidant properties in roots of Salvia miltiorrhiza (Danshen).
Molecules
PUBLISHED: 04-29-2014
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Danshen, the dried roots of Salvia miltiorrhiza, is an extremely valued Traditional Chinese Medicine. Previously, we have demonstrated that salvianolic acid B (SaB), the important bioactive ingredient in this herb, was a post-harvest product. Here, we further reported that all salvianolic acids (SAs) in the roots were post-harvest products of the drying process. In addition, the results of various radical scavenging activity assays, including lipid peroxidation (1), DPPH (2), hydroxyl (3) and superoxide (4), were significantly increased along with the accumulation of total salvianolic acids in the process. The contents of chemical targets and antioxidant activities both reached the highest value under thermal treatment at 130 °C for 80 min. In this dehydration period, contents of SaB, and sum of nine SAs increased from 0.01% to 5.51%, and 0.20% to 6.61%; and IC50 of antioxidant activity decreased from 4.85 to 2.69 (1); 7.75 to 0.43 (2); 2.57 to 1.13 (3) and 17.25 to 1.10 mg/mL. These results further supported the hypothesis that the newly harvested plant roots were still physiologically active and the secondary metabolites might be produced due to dehydration stress after harvest. Our findings supplied an important and useful theoretical basis for promoting the quality of Danshen and other medicinal plant materials.
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Determination and fingerprint analysis of steroidal saponins in roots of Liriope muscari (Decne.) L. H. Bailey by ultra high performance liquid chromatography coupled with ion trap time-of-flight mass spectrometry.
J Sep Sci
PUBLISHED: 03-13-2014
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Liriope muscari (Decne.) L. H. Bailey is a well-known traditional Chinese medicine used for treating cough and insomnia. There are few reports on the quality evaluation of this herb partly because the major steroid saponins are not readily identified by UV detectors and are not easily isolated due to the existence of many similar isomers. In this study, a qualitative and quantitative method was developed to analyze the major components in L. muscari (Decne.) L. H. Bailey roots. Sixteen components were deduced and identified primarily by the information obtained from ultra high performance liquid chromatography with ion-trap time-of-flight mass spectrometry. The method demonstrated the desired specificity, linearity, stability, precision, and accuracy for simultaneous determination of 15 constituents (13 steroidal glycosides, 25(R)-ruscogenin, and pentylbenzoate) in 26 samples from different origins. The fingerprint was established, and the evaluation was achieved using similarity analysis and principal component analysis of 15 fingerprint peaks from 26 samples by ultra high performance liquid chromatography. The results from similarity analysis were consistent with those of principal component analysis. All results suggest that the established method could be applied effectively to the determination of multi-ingredients and fingerprint analysis of steroid saponins for quality assessment and control of L. muscari (Decne.) L. H. Bailey.
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Roles of glucose transporter-1 and the phosphatidylinositol 3?kinase/protein kinase B pathway in cancer radioresistance.
Mol Med Rep
PUBLISHED: 01-28-2014
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The mechanisms underlying cancer radioresistance remain unclear. Several studies have found that increased glucose transporter?1 (GLUT?1) expression is associated with radioresistance. Recently, the phosphatidylinositol 3-kinase (PI3K)/protein kinase B (Akt) pathway was reported to be involved in the control of GLUT?1 trafficking and activity. Activation of the PI3K/Akt pathway may itself be associated with cancer radioresistance. Thus, increasing attention has been devoted to the effects of modifying the expression of GLUT?1 and the PI3K/Akt pathway on the increase in the radiosensitivity of cancer cells. This review discusses the importance of the association between elevated expression of GLUT?1 and activation of the PI3K/Akt pathway in the development of radioresistance in cancer.
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Fluorodeoxyglucose uptake in laryngeal carcinoma is associated with the expression of glucose transporter-1 and hypoxia-inducible-factor-1? and the phosphoinositide 3-kinase/protein kinase B pathway.
Oncol Lett
PUBLISHED: 01-23-2014
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High fluorodeoxyglucose (FDG) uptake by human carcinomas, including head and neck cancers, is associated with a poor prognosis. Glucose transporter-1 (Glut-1) is believed to be an intrinsic marker of hypoxia in malignant tumors. The expression of hypoxia-inducible factor-1? (HIF-1?) and correlated target genes, including Glut-1, is regulated by the phosphoinositide 3-kinase/protein kinase B (PI3K/Akt) pathway. However, it remains unclear whether the PI3K/Akt signaling pathway is involved in regulating FDG uptake directly. In the present study, 24 consecutive patients with laryngeal carcinoma were examined pre-operatively and the standardized uptake values (SUV) of the laryngeal carcinomas were determined. Glut-1, HIF-1?, PI3K and phosphorylated-Akt (p-Akt) expression was detected by immunohistochemical staining of paraffin sections from the tumor specimens. Associations among SUVmax, Glut-1, HIF-1?, PI3K and p-Akt protein expression and the other clinical parameters were analyzed. The univariate analyses revealed a significantly shorter survival time along with higher HIF-1? (P=0.018) and PI3K (P=0.008) expression, but the survival time was not significantly correlated with Glut-1 or p-Akt expression. The multivariate analysis demonstrated that higher SUVmax (P=0.043) and PI3K expression (P=0.012) were significantly correlated with a poor survival time. Spearman's rank analysis showed significant correlations between SUVmax and HIF-1? (r=0.577; P=0.003), PI3K (r=1.0; P<0.0001) and p-Akt (r=0.577; P=0.003) expression. PI3K was associated with poorly- and moderately-differentiated laryngeal carcinoma (P=0.012). In conclusion, a high SUVmax indicates a poor prognosis for laryngeal carcinoma. Also, a high SUVmax may be associated with the increased expression of Glut-1, HIF-1?, PI3K and p-Akt.
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Metastatic squamous cell carcinoma of the gingiva appearing as a solitary branchial cyst carcinoma: diagnostic role of PET/CT.
Int J Clin Exp Pathol
PUBLISHED: 01-01-2014
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We herein present a case of a left cervical cystic mass, for which the initial pathological diagnosis was branchial cleft cyst carcinoma (following complete mass excision). Thorough postoperative examinations, including with FDG positron emission tomography/computed tomography (PET/CT), revealed a primary tumor in the retromolar region of the left mandible. A 52-year-old female presented with a 2-month history of a painless, progressively enlarged left-sided neck mass. Fine-needle aspiration biopsy suggested a branchial cleft cyst. Physical examination revealed a 3 × 3-cm smooth, tender mass in the upper-left neck and anterior border of the sternocleidomastoid muscle. Examination using nasendoscopy and a strobolaryngoscope revealed no abnormalities of the nasal cavity, nasopharynx, oropharynx, hypopharynx or larynx. MRI of the neck revealed a solitary, round, cystic mass under the left parotid gland. The mass was excised completely. Pathologic results indicated a branchial cleft cyst carcinoma. According to the diagnostic criteria for a branchial cleft cystic carcinoma, PET/CT was performed to detect the occult primary site. PET/CT revealed high FDG uptake in the tooth root of the left mandible. Frozen sections of the mass were indicative of moderate, differentiated squamous cell carcinoma. The carcinoma in the retromolar region of the left mandible was locally excised under general anesthesia. A partial left maxillectomy, partial mandibulectomy, and left radical neck dissection were performed. The patient received postoperative concurrent chemoradiotherapy, and was disease-free at the 8-month follow-up. True branchial cleft cyst carcinoma is rare: once diagnosed, it should be distinguished from metastatic cystic cervical lymph and occult primary carcinoma. FDG PET/CT is useful in the identification of occult primary tumor.
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Obstructive sleep apnea syndrome caused by uncommon tumors of the upper aerodigestive tract.
Int J Clin Exp Pathol
PUBLISHED: 01-01-2014
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Obstructive sleep apnea syndrome (OSAS) is always caused by anatomic abnormalities, including nasal cavity, pharynx, and neuromuscular dysfunctions, leading to airway narrowing. OSAS associated with a mass in the aerodigestive tract is rare. In the present study, we report OSAS caused by 9 cases of preoperative uncommon tumors in the aerodigestive tract. Two tumors in the parapharyngeal space were pleomorphic adenoma, one oropharyngeal tumor was mucoepidermoid carcinoma, one tumor in the right tonsil was schwannoma, and five tumors were non-Hodgkin's lymphoma (NHL). Of the five NHL cases, one in the nasopharynx was diffuse large B-cell lymphoma, two were mantle cell lymphoma, one was chronic lymphocytic leukemia/small lymphocytic lymphoma, and one was NHL. Tumors in the aerodigestive tract should be considered in the differential diagnosis of OSAS upon exacerbation of snoring or sudden gasping. Further examinations should be performed, including a routine workup (computed tomography (CT) and magnetic resonance imaging) and positron emission tomography/CT.
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Spindle cell rhabdomyosarcoma in the hypopharynx of an adult.
Int J Clin Exp Pathol
PUBLISHED: 01-01-2014
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We herein present the first-reported case in the English-language literature of a spindle cell rhabdomyosarcoma in the hypopharynx of an adult. A 58-year-old male presented with a progressive, 7-month history of discomfort during swallowing, accompanied by hoarseness for 1 month. Computed tomography and magnetic resonance imaging revealed a tumor in the left pyriform sinus; frozen section evaluation suggested that it was rhabdomyosarcoma. The tumor was removed completely via left lateral cervical incision. Section margins were negative, and laryngeal function was preserved. The postoperative pathological results indicated spindle cell rhabdomyosarcoma. The patient received postoperative radiotherapy (6,000 cGy in 200 cGy fractions, delivered over 30 days). However, he was also required to undergo urgent tracheostomy due to severe laryngeal obstruction, precipitated by laryngeal edema, 3 months subsequent to radiotherapy. The patient was free of disease 25 months postoperatively but is still unable to close his tracheostomy.
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[Difference evaluation of three kinds of root of Aconitum carmichaelii in Sichuan based on UPLC analysis of six alkaloids and chemometrics].
Zhongguo Zhong Yao Za Zhi
PUBLISHED: 12-31-2013
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An ultra performance liquid chromatography (UPLC) method was established and validated to simultaneously determine the contents of six aconitum alkaloids in mother, daughter and fibrous roots of 19 batches of Aconitum carmichaelii from Sichuan province. The separation of the six alkaloids was achieved on a ACQUITY UPLC BEH C18 (2.1 mm x 100 mm, 1.7 microm) column at 40 degrees C with a mobile phase consisting of acetonitrile in 30 mmol x L(-1) ammonium acetate buffer solution (adjusted to pH 10.0 with aqueous ammonia) in gradient mode. The data and plots showed that the six aconitum alkaloids have different distributions. Four aconitum alkaloids were almost same in mother and daughter root except benzoylmesaconine and mesaconitine, while the fibrous root differed from the other two roots. The comparisons of significant differences of six aconitum alkaloids between the mother and daughter roots definitely demonstrated that benzoylmesaconine and mesaconitine were the representative components. The 38 detecting samples were classified as two clusters by hierarchical clustering analysis (HCA) and principle component analysis (PCA), the results indicated that the mother root was different from the daughter root on chemical material basis. The study might contribute to the reasonable clinical application of A. carmichaelii.
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[Analysis of K76T Pfcrt genetic polymorphism in imported Plasmodium falciparum].
Zhongguo Xue Xi Chong Bing Fang Zhi Za Zhi
PUBLISHED: 12-24-2013
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To identify the incidence of the K76T mutation in Pfcrt genes of imported Plasmodium falciparum.
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[Ecological behavior comparison between Anopheles pseudowillmori and A. willmori in villages with malaria outbreaks in Motuo County, Tibet Autonomous Region].
Zhongguo Xue Xi Chong Bing Fang Zhi Za Zhi
PUBLISHED: 12-24-2013
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To understand the ecological behaviors of Anopheles pseudowillmori and A. willmori in medium or high altitude areas of Motuo County, Tibet Autonomous Region, and their transmission potential for malaria.
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Protein inhibitor of activated STAT 1 (PIAS1) is identified as the SUMO E3 ligase of CCAAT/enhancer-binding protein ? (C/EBP?) during adipogenesis.
Mol. Cell. Biol.
PUBLISHED: 09-23-2013
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It is well recognized that PIAS1, a SUMO (small ubiquitin-like modifier) E3 ligase, modulates such cellular processes as cell proliferation, DNA damage responses, and inflammation responses. Recent studies have shown that PIAS1 also plays a part in cell differentiation. However, the role of PIAS1 in adipocyte differentiation remains unknown. CCAAT/enhancer-binding protein ? (C/EBP?), a major regulator of adipogenesis, is a target of SUMOylation, but the E3 ligase responsible for the SUMOylation of C/EBP? has not been identified. The present study showed that PIAS1 functions as a SUMO E3 ligase of C/EBP? to regulate adipogenesis. PIAS1 expression was significantly and transiently induced on day 4 of 3T3-L1 adipocyte differentiation, when C/EBP? began to decline. PIAS1 was found to interact with C/EBP? through the SAP (scaffold attachment factor A/B/acinus/PIAS) domain and SUMOylate it, leading to increased ubiquitination and degradation of C/EBP?. C/EBP? became more stable when PIAS1 was silenced by RNA interference (RNAi). Moreover, adipogenesis was inhibited by overexpression of wild-type PIAS1 and promoted by knockdown of PIAS1. The mutational study indicated that the catalytic activity of SUMO E3 ligase was required for PIAS1 to restrain adipogenesis. Importantly, the inhibitory effect of PIAS1 overexpression on adipogenesis was rescued by overexpressed C/EBP?. Thus, PIAS1 could play a dynamic role in adipogenesis by promoting the SUMOylation of C/EBP?.
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[A cross-sectional study on iodine nutrition in general population from Zhejiang province, China].
Zhonghua Liu Xing Bing Xue Za Zhi
PUBLISHED: 09-11-2013
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To understand the current status of iodine nutrition among the community residents to compare the level of iodine nutrition in different areas and groups of populations in Zhejiang province.
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Anticancer mechanism of apigenin and the implications of GLUT-1 expression in head and neck cancers.
Future Oncol
PUBLISHED: 08-29-2013
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Apigenin, a natural phytoestrogen flavonoid, has potential biological effects, including antioxidative, anti-in?ammatory and anticancer activities. The mechanisms of anticancer activities of apigenin are unknown. Some studies have found that apigenin inhibits GLUT-1 mRNA and protein expression in cancer cells. Thus, we hypothesized that apigenin exerts similar effects on head and neck cancers through its inhibition of GLUT-1 expression. In this article, we review the anticancer mechanism of apigenin and the implications of GLUT-1 expression in head and neck cancers. In addition, we describe the current state of knowledge about the relationship between apigenin and GLUT-1 expression in head and neck cancers.
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Simultaneous Quantitative Determination of Six Active Components in Traditional Chinese Medicinal Preparation Cerebralcare Granule(R) by RP-HPLC Coupled with Diode Array Detection for Quality Control.
J Chromatogr Sci
PUBLISHED: 08-08-2013
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A simple, accurate and reliable method for the simultaneous separation and determination of six active components (protocatechuic acid, chlorogenic acid, caffeic acid, paeoniflorin, ferulic acid and rosmarinic acid) in traditional Chinese medicinal preparation Cerebralcare Granule(®) (CG) was developed using reverse-phase high-performance liquid chromatography coupled with diode array detector detection. The chromatographic separation was performed on a Hypersil GOLD C18 column with aqueous formic acid (0.1%, v/v) and acetonitrile as mobile phase at a flow rate of 0.2 ml/min at 30°C. Because of the different UV characteristics of these components, change detection wavelength method was used for quantitative analysis. All of the analytes showed good linearity (r > 0.9992). The established method showed good precision and relative standard deviations (%) for intra-day and inter-day variations of 0.15-1.81 and 0.11-1.98%, respectively. The validated method was successfully applied to the simultaneously determination of six active components in CG from different batches.
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[Changes of pyrethroid resistance and P450 monooxygenase activity with age in Anopheles sinensis in Huainan City, Anhui Province, China].
Zhongguo Xue Xi Chong Bing Fang Zhi Za Zhi
PUBLISHED: 07-31-2013
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To study the effect of mosquito age on the pyrethroid resistance and P450 monooxygenase activity in Anopheles sinensis in Huainan City, Anhui Province, China, as well as the molecular mechanism of pyrethroid resistance.
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Expression of hypoxia-related markers in inflammatory myofibroblastic tumors of the head and neck.
World J Surg Oncol
PUBLISHED: 06-19-2013
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The etiology of inflammatory myofibroblastic tumors (IMTs) is controversial and the prognosis is unpredictable. Previous studies have not investigated the expression of hypoxia-related markers in IMTs.
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Transactivation of Atg4b by C/EBP? promotes autophagy to facilitate adipogenesis.
Mol. Cell. Biol.
PUBLISHED: 06-10-2013
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Autophagy is a highly conserved self-digestion pathway involved in various physiological and pathophysiological processes. Recent studies have implicated a pivotal role of autophagy in adipocyte differentiation, but the molecular mechanism for its role and how it is regulated during this process are not clear. Here, we show that CCAAT /enhancer-binding protein ? (C/EBP?), an important adipogenic factor, is required for the activation of autophagy during 3T3-L1 adipocyte differentiation. An autophagy-related gene, Atg4b, is identified as a de novo target gene of C/EBP? and is shown to play an important role in 3T3-L1 adipocyte differentiation. Furthermore, autophagy is required for the degradation of Klf2 and Klf3, two negative regulators of adipocyte differentiation, which is mediated by the adaptor protein p62/SQSTM1. Importantly, the regulation of autophagy by C/EBP? and the role of autophagy in Klf2/3 degradation and in adipogenesis are further confirmed in mouse models. Our data describe a novel function of C/EBP? in regulating autophagy and reveal the mechanism of autophagy during adipocyte differentiation. These new insights into the molecular mechanism of adipose tissue development provide a functional pathway with therapeutic potential against obesity and its related metabolic disorders.
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Poor outcome of comprehensive therapy in a case of laryngeal synovial sarcoma.
Radiol Oncol
PUBLISHED: 06-01-2013
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Synovial sarcoma is common in the extremities. Our search revealed only 17 cases of synovial sarcoma of the larynx in the English-language literature.
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Students awareness of malaria at the beginning of national malaria elimination programme in China.
Malar. J.
PUBLISHED: 05-28-2013
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In the battle against malaria in China, the rate of elementary and high school students awareness on malaria knowledge is an important index for malaria elimination, but only rare data is available. This study aimed to investigate the level of malaria awareness in students at elementary and high schools in malaria endemic areas of China, and to provide the baseline information for the malaria elimination.
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Development of strategies for screening, predicting, and diagnosing intrauterine HBV infection in infants born to HBsAg positive mothers.
J. Med. Virol.
PUBLISHED: 05-13-2013
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This study aimed at developing strategies for screening, predicting, and diagnosing intrauterine HBV infection in infants born to HBsAg positive mothers. A total of 1,360 infants born to 1,355 HBsAg positive mothers were followed for 1 year. All newborn infants received active and passive immunization within 24 hr after birth. Maternal and infant blood samples were collected and tested for the status of serum HBsAg, HBeAg, and HBV DNA positivity. The accuracy of infant HBsAg positivity, HBV DNA positivity, HBsAg and HBV DNA double positivity, and HBsAg and/or HBV DNA positivity at birth in the diagnosis of intrauterine HBV infection was evaluated by receiver operating characteristic curve analysis. Of 1,360 infants, 145 tested positive for HBsAg and/or HBV DNA at birth. Twenty-one (1.5%) infants, who were diagnosed with intrauterine HBV infection, showed HBsAg positivity from birth to 7 and 12 months of age. Infant HBsAg positivity at birth had the highest sensitivity in predicting intrauterine HBV infection, while neonatal HBsAg and HBV DNA double positivity had the highest specificity. These findings suggest that infants, who were born to HBsAg positive mothers and were positive for both HBsAg and HBV DNA at birth, may be at a higher risk for intrauterine HBV infection. HBsAg positivity at birth may be a good marker for screening intrauterine HBV infection. Infant HBsAg positivity both at birth and 7 months of age may be used as a diagnostic criterion to simplify diagnostic procedures and improve diagnostic efficiency.
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Value of helical computed tomography in the early diagnosis of esophageal foreign bodies in adults.
Am J Emerg Med
PUBLISHED: 05-06-2013
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To investigate the relationship between early use of computed tomography (CT) and complications associated with esophageal foreign body impaction in adults.
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[Analysis of urine iodine level and its influencing factors in Zhejiang from 2009 to 2011].
Zhonghua Yu Fang Yi Xue Za Zhi
PUBLISHED: 04-23-2013
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To evaluate the iodine nutrition level of population in Zhejiang province and to analyze the relevant influencing factors from 2009 to 2011.
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Interleukin-17A expression in patients with chronic rhinosinusitis and its relationship with clinical features.
J. Int. Med. Res.
PUBLISHED: 04-18-2013
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To investigate the expression of interleukin (IL) 17A in Chinese patients with chronic rhinosinusitis (CRS) and analyse its correlation with clinical parameters.
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Simultaneous determination of five phenolic components and paeoniflorin in rat plasma by liquid chromatography-tandem mass spectrometry and pharmacokinetic study after oral administration of Cerebralcare granule(®).
J Pharm Biomed Anal
PUBLISHED: 04-12-2013
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A simple, sensitive and selective high-performance liquid chromatography electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS) method was developed for simultaneous determination and pharmacokinetic study of six active components, protocatechuic acid, chlorogenic acid, caffeic acid, ferulic acid rosmarinic acid and paeoniflorin in rat plasma after oral administration of Cerebralcare granule(®) for the first time. The method involves a simple liquid-liquid extraction with ethyl acetate. The separation was performed on a Luna C18 column (2.0×100mm i.d., 3.0?m, particle, Phenomenex, USA) with gradient elution using a mobile phase composed of acetonitrile and water (containing 0.1% formic acid) at a flow rate of 0.2ml/min. Electrospray ionization (ESI) in negative ion mode and selective reaction monitoring (SRM) was used for the quantification of six active components and internal standard (IS, Chloroamphenicol). The method was linear for all analytes over investigated range with all correlation coefficients greater than 0.9914. The lower limits of quantification (LLOQ) were 1.0ng/ml for protocatechuic acid, 1.0ng/ml for chlorogenic acid, 1.0ng/ml for caffeic acid, 5.0ng/ml for ferulic acid, 1.5ng/ml for rosmarinic acid and 6.0ng/ml for paeoniflorin, respectively. The intra- and inter-day precisions (R.S.D.%) were less than 6.60% and 11.68%, and accuracy (RE %) between -3.26% and 1.13% (n=6). The developed method was applied for the first time to the pharmacokinetic study of protocatechuic acid, chlorogenic acid, caffeic acid, ferulic acid, rosmarinic acid and paeoniflorin in rat plasma after oral administration of Cerebralcare granule(®).
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Glucose transporter-1 expression in CD133+ laryngeal carcinoma Hep-2 cells.
Mol Med Rep
PUBLISHED: 04-10-2013
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CD133 is a useful putative marker of cancer stem cells (CSCs) in human laryngeal tumors. Numerous studies have demonstrated that CD133+ CSCs possess higher clonogenicity, invasiveness and tumorigenesis compared with CD133- cells. Recently, interest in the Warburg effect in the microenvironment of CSCs has escalated. The Warburg effect dictates that cancer cells rely on glycolysis rather than oxidative phosphorylation under aerobic conditions. In numerous cancer cells, glucose is used mainly for the glycolytic pathway. Stem cells express high levels of glycolytic enzymes and rely mostly on glycolysis to meet their energy demands. Glucose is transported through cell membranes by glucose transporters (Glut). Studies of Glut-1 expression in CSCs are limited. In the present study, we investigated the proliferation of CD133+ Hep-2 cells and whether Glut-1 is expressed in laryngeal carcinoma CD133+ Hep-2 cells. Real-time reverse transcription-polymerase chain reaction (RT-PCR) demonstrated that the size of the CD133 product was 213 bp. Dissociation curve analysis demonstrated only the expected peaks at 82.1?C for CD133. The mean ?Ct of CD133 expression was 10.98. Prior to isolation, the CD133+ fraction was 1.2% by fluorescence-activated cell sorting (FACS) analysis. Following isolation, the CD133+ fraction was increased to 76.1%. Successive tests also demonstrated that cells grew well following isolation. The proliferation of CD133+ and CD133- cells was not different during the first 3 days (P>0.05). From day 4, the proliferation capacity of CD133+ cells in vitro was higher than that of CD133- cells (P<0.05). The mean ?Ct of Glut-1 mRNA expression was 1.78 for CD133+ cells and 1.00 for CD133- cells (P<0.05). The mean Glut-1 protein values in CD133+ and CD133- Hep-2 cells relative to ?-tubulin were 0.48 ± 0.02 and 0.21 ± 0.03 (µg/µl), respectively (P<0.05). In conclusion, CD133+ cells demonstrated higher proliferation. Glut-1 mRNA and protein levels were higher in CD133+ than in CD133- cells. Our results suggest that Glut-1 is important in the energy supply of laryngeal CD133+ Hep-2 cells and Glut-1 may represent a potential therapeutic target for the inhibition of the proliferation of laryngeal CSCs.
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A potential threat to malaria elimination: extensive deltamethrin and DDT resistance to Anopheles sinensis from the malaria-endemic areas in China.
Malar. J.
PUBLISHED: 04-04-2013
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Insecticide resistance in malaria vectors is a growing concern in many countries and requires immediate attention because of the limited chemical arsenal available for vector control. There is lack of systematic and standard monitoring data of malaria vector resistance in the endemic areas, which is essential for the ambitious goal of malaria elimination programme of China.
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Palatine Tonsillar Metastasis of Small-Cell Neuroendocrine Carcinoma from the Lung Detected by FDG-PET/CT After Tonsillectomy: A Case Report.
Iran J Radiol
PUBLISHED: 02-20-2013
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Metastasis from a malignant tumor to the palatine tonsils is rare, accounting for only 0.8% of all tonsillar tumors, with only 100 cases reported in the English-language literature. Various malignant lung carcinomas may metastasize to the tonsils. A few cases of tonsillar metastasis from neuroendocrine lung carcinoma have been reported. A 67-year-old female underwent a right tonsillectomy because of a sore throat and an enlarged right tonsil. The postoperative pathology showed right tonsillar small cell neuroendocrine carcinoma (SCNC). Fluorodeoxyglucose (FDG) positron emission tomography (PET)/computed tomography (CT) demonstrated metabolic activity in the lower lobe of the right lung. In addition, hypermetabolic foci were noted in the lymph nodes of the right neck and mediastinum. A needle biopsy of the pulmonary mass showed SCNC. The patient received chemotherapy and died of multiple distant metastases after 6 months. This is the first report using PET/CT to evaluate tonsillar metastasis from lung SCNC.
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Prospective multicenter clinical trial of Chinese herbal formula JZQG (Jiangzhuoqinggan) for hypertension.
Am. J. Chin. Med.
PUBLISHED: 01-23-2013
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A prospective multicenter clinical trial was conducted to compare the beneficial effects of a Chinese herbal medicine formula Jiangzhuoqinggan (JZQG) and western antihypertension drug irbesartan. JZQG is mainly composed of rhubarb, coptis, cassia, and uncaria. A total of 240 patients with mild to moderate hypertension were enrolled in the trial. Patients were assigned into two groups after screening: JZQG group and the irbesartan group. After four weeks of treatment, we compared the changes in routine blood pressure, 24 h ambulatory blood pressure, and waist circumference. There was a significant reduction in systolic blood pressure and diastolic blood pressure in the JZQG group (both p < 0.01). There were no significant differences between the reduction of systolic and diastolic blood pressures in the two treatment groups. From the 24 h ambulatory blood pressure measurement, the JZQG group showed a greater reduction in both systolic and diastolic blood pressures (in both daytime and nighttime) than the irbesartan group. Furthermore, there was a significant difference in waist circumference in the JZQG group (1.51 cm reduction; P < 0.05) but not the irbesartan group (0.42 cm). Thus, the JZQG formula may have therapeutic value in patients with both hypertension and metabolic syndrome.
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Determination of ginsenoside Rc in rat plasma by LC-MS/MS and its application to a pharmacokinetic study.
J. Chromatogr. B Analyt. Technol. Biomed. Life Sci.
PUBLISHED: 01-16-2013
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Ginsenoside Rc (GRc) is a potential pharmacologically active ingredient isolated from ginseng (Panax ginseng C.A. Meyer, Araliaceae). A simple, rapid and sensitive method for determination of GRc in rat plasma was developed based on liquid chromatography-tandem mass spectrometry (LC-MS/MS). The analyte and internal standard (IS), ginsenoside Rb(1) (GRb(1)), were extracted from plasma with n-butanol and chromatographied on a C(18) column eluted with a mobile phase of methanol and water containing 0.1% formic acid. The detection was performed by positive ion electrospray ionization in selective reaction monitoring mode (SRM), monitoring the transitions m/z 1101.6?789.3 and m/z 1131.7?364.7 for GRc and IS, respectively. The assay was linear over the concentration range of 5-5000 ng/mL with a limit of quantitation (LOQ) of 5 ng/mL. The accuracy was between 86.7% and 114.9%, and the precision was less than 9.7%. This method was successfully applied to investigate the pharmacokinetic study of GRc in rats after intravenous (2mg/kg) and oral (20mg/kg) administration, and the result showed that the ginsenoside was poorly absorbed with an absolute bioavailability being approximately 0.17%.
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Changing malaria transmission and implications in China towards National Malaria Elimination Programme between 2010 and 2012.
PLoS ONE
PUBLISHED: 01-01-2013
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Towards the implementation of national malaria elimination programme in China since 2010, the epidemiology of malaria has changed dramatically, and the lowest malaria burden was achieved yearly. It is time to analyze the changes of malaria situation based on surveillance data from 2010 to 2012 to reconsider the strategies for malaria elimination.
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Effect of antisense oligodeoxynucleotides glucose transporter-1 on enhancement of radiosensitivity of laryngeal carcinoma.
Int J Med Sci
PUBLISHED: 01-01-2013
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Laryngeal carcinomas always resist to radiotherapy. Hypoxia is an important factor in radioresistance of laryngeal carcinoma. Glucose transporter-1 (GLUT-1) is considered to be a possible intrinsic marker of hypoxia in malignant tumors. We speculated that the inhibition of GLUT-1 expression might improve the radiosensitivity of laryngeal carcinoma.
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Differentiating laryngeal carcinomas from precursor lesions by diffusion-weighted magnetic resonance imaging at 3.0 T: a preliminary study.
PLoS ONE
PUBLISHED: 01-01-2013
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Diffusion-weighted magnetic resonance imaging (DWI) has been introduced in head and neck cancers. Due to limitations in the performance of laryngeal DWI, including the complex anatomical structure of the larynx leading to susceptibility effects, the value of DWI in differentiating benign from malignant laryngeal lesions has largely been ignored. We assessed whether a threshold for the apparent diffusion coefficient (ADC) was useful in differentiating preoperative laryngeal carcinomas from precursor lesions by turbo spin-echo (TSE) DWI and 3.0-T magnetic resonance.
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[Investigation on Anopheles species and their composition in villages at different altitudes of Motuo County, Tibet Autonomous Region].
Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi
PUBLISHED: 10-07-2011
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To study the anopheline species and composition in villages at different altitudes, Muotuo County.
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[Application of nested PCR in diagnosis of imported malaria].
Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi
PUBLISHED: 08-10-2011
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To evaluate the usefulness of nested PCR method in the diagnosis of imported malaria.
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[Malaria situation in the Peoples Republic Of China in 2009].
Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi
PUBLISHED: 08-10-2011
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Totally 14,491 malaria cases and 59 741 suspected cases with 12 deaths were reported by the annual case reporting system in 1171 counties of 23 Provinces/Municipalities/Autonomous Regions (P/M/A) in 2009, and the annual incidence was 0.11/10,000. Through the internet reporting system, 14,140 malaria cases were reported from 956 counties of 30 P/M/ A. The number of malaria cases and the rank of P/M/A were basically in concordance in the two systems. Among the 1171 counties with reported malaria cases, 4 counties with an incidence of more than 10/10,000 distributed in Yunnan (3 counties) and Tibet (1). There were 59 counties in which the malaria incidence was between 1/ 10,000 and 10/10,000. A total of 1027 Plasmodium falciparum malaria cases accounted for 7.1% of the total cases, of which 87.3% (897) were imported cases reported in 326 counties/cities of 21 P/M/A. Indigenous falciparum malaria was found in 18 counties/cities of Yunnan and Hainan Provinces, of which 16 counties/cities were in Yunnan and 2 counties/cities were in Hainan, 3 or 6 less respectively in comparison to that of 2008. No focal outbreaks occurred in the whole country in 2009. Although a considerable decrease in malaria incidence contributed to the implementation of the National Malaria Control Program and the project supported by the Global Fund to Fight AIDS, Tuberculosis and Malaria, Yunnan and Hainan Provinces are still the relatively high transmission areas. Yunnan ranked No.2 in the country in terms of the number of cases, while Hainan ranked down to No.6 by malaria incidence in 2009. Three thousand nine hundred and twenty two malaria cases were reported from the two provinces in 2009, accounting for 27.1% of the total reported cases in the country. There were 3 237 cases with 3 deaths reported from Yunnan, the incidence was 0.96/10,000, a decrease of 17.2% than that in the last year. Among the reported cases, 567 were falciparum malaria with 49.7% imported cases. The number of reported cases in Hainan was 685, with an incidence of 0.79/10,000, 63.8% decrease than the last year. In central China, the resurgence of malaria was suppressed and the number of malaria cases was considerably decreased in 2009, but the incidence in Anhui Province has still been the first in the country. A total of 5 918 malaria cases were reported from Anhui Province in 2009, accounting for 40.8% of the total cases in the country, with an incidence of 1.19/10,000 which decreased by 50.8% than that in 2008. The number of reported cases in Henan Province was 1 611, decreased by 40.8% in incidence. Hubei Province reported 709 malaria cases with an incidence of 0.12/10,000, decreased by 34.8%. There were 389 cases reported from Jiangsu Province, decreased by 41.8% compared to that in 2008. Cases reported from other P/M/A occupied about 13.4% of the total. Above hundred were reported from Shandong and less than 100 cases were reported from each of Fujian, Chongqing, Shanghai, Jiangxi, Liaoning, Shaanxi, Jilin, Heilongjiang, Gansu and Tibet in 2009. In summary, the re-emergence of malaria has been restrained through several years efforts, but malaria is still an important problem of public health in China, especially in the southern and central parts. Yunnan and Hainan still face the spread of Plasmodium falciparum, especially imported malaria in the border areas. In the central parts of the country, Anhui Province is still with the highest incidence in 2009. In addition, imported malaria cases in those provinces with a low transmission or without local transmission may become a new challenge to the National Malaria Control Program.
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In silico analysis of phytoene synthase and its promoter reveals hints for regulation mechanisms of carotenogenesis in Duanliella bardawil.
Bioinformatics
PUBLISHED: 06-27-2011
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Previous researches showed that phytoene synthase (Psy) from Dunaliella bardawil is the first regulatory point in carotenogenesis. We hypothesize certain interactions between the environmental stress factors and the regulatory sequences of Psy in D.bardawil (DbPsy). Consequently, LA PCR-based genomic walking approach was performed for isolation of psy promoter and terminator, respectively. The obtained nucleic acid sequences and the corresponding protein structure of DbPsy were analyzed and predicted using various bioinformatics tools. Finally, we presented some hints for the regulation mechanisms of DbPsy at the molecular level according to the computed results.
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Comparing efficacy of ?1D-receptor antagonist naftopidil and ?1A/D-receptor antagonist tamsulosin in management of distal ureteral stones.
World J Urol
PUBLISHED: 04-23-2011
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To compare the efficacy of ?(1)D-receptor antagonist Naftopidil and ?(1)A/D-receptor antagonist Tamsulosin in management of distal ureteral stones.
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Factors associated with recurrence and therapeutic strategies for sinonasal Rosai-Dorfman disease.
Head Neck
PUBLISHED: 03-31-2011
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The purpose of this study was to explore the factors associated with the recurrence of Rosai-Dorfman disease (RDD) in the nasal cavity and paranasal sinus.
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SemiHS: an iterative semi-supervised approach for predicting protein-protein interaction hot spots.
Protein Pept. Lett.
PUBLISHED: 02-15-2011
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Protein-protein interaction hot spots, as revealed by alanine scanning mutagenesis, make dominant contributions to the free energy of binding. Since mutagenesis experiments are expensive and time-consuming, the development of computational methods to identify hot spots is becoming increasingly important. In this study, by using a new combination of sequence, structure and energy features, we propose an iterative semi-supervised algorithm, SemiHS, to incorporate unlabeled data to improve the accuracy of hot spots prediction when sufficient training data is un-available and to overcome the imbalanced data problem. We evaluate the predictive power of SemiHS on a labeled set of 265 alanine-mutated interface residues in 17 complexes and a large unlabeled set of 2465 interface residues with 10-fold cross validation, and get an AUC score of 0.85, with a sensitivity of 0.70 and a specificity of 0.87, which are better than those of the existing methods. Moreover, we validate the proposed method by an independent test and obtain encouraging results.
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[Penile and scrotal skin flaps: first choice for urethroplasty in the treatment of hypospadias].
Zhonghua Nan Ke Xue
PUBLISHED: 11-25-2010
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To explore the feasibility of the treatment of hypospadias with penile and scrotal skin flaps.
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Mapping and predicting malaria transmission in the Peoples Republic of China, using integrated biology-driven and statistical models.
Geospat Health
PUBLISHED: 11-17-2010
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The purpose of this study was to deepen our understanding of Plasmodium vivax malaria transmission patterns in the Peoples Republic of China (P.R. China). An integrated modeling approach was employed, combining biological and statistical models. A Delphi approach was used to determine environmental factors that govern malaria transmission. Key factors identified (i.e. temperature, rainfall and relative humidity) were utilized for subsequent mapping and modeling purposes. Yearly growing degree days, annual rainfall and effective yearly relative humidity were extracted from a 15-year time series (1981-1995) of daily environmental data readily available for 676 locations in P.R. China. A suite of eight multinomial regression models, ranging from the null model to a fully saturated one were constructed. Two different information criteria were used for model ranking, namely the corrected Akaikes information criterion and the Bayesian information criterion. Mapping was based on model output data, facilitated by using ArcGIS software. Temperature was found to be the most important environmental factor, followed by rainfall and relative humidity in the Delphi evaluation. However, relative humidity was found to be more important than rainfall and temperature in the ranking list according to the three single environmental factor regression models. We conclude that the distribution of the mosquito vector is mainly related to relative humidity, which thus determines the extent of malaria transmission. However, in regions with relative humidity >60%, temperature is the major driver of malaria transmission intensity. By integrating biology-driven models with statistical regression models, reliable risk maps indicating the distribution of transmission and the intensity can be produced. In a next step, we propose to integrate social and health systems factors into our modeling approach, which should provide a platform for rigorous surveillance and monitoring progress towards P. vivax malaria elimination in P.R. China.
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Geographical, meteorological and vectorial factors related to malaria re-emergence in Huang-Huai River of central China.
Malar. J.
PUBLISHED: 08-10-2010
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Malaria still represents a significant public health problem in China, and the cases dramatically increased in the areas along the Huang-Huai River of central China after 2001. Considering spatial aggregation of malaria cases and specific vectors, the geographical, meteorological and vectorial factors were analysed to determine the key factors related to malaria re-emergence in these particular areas.
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Stunting and soil-transmitted-helminth infections among school-age pupils in rural areas of southern China.
Parasit Vectors
PUBLISHED: 07-10-2010
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Stunting and soil-transmitted helminth (STH) infections including ascariasis, trichuriasis and hookworm remain major public health problems in school-age pupils in developing countries. The objectives of this study were to determine the prevalence of stunting for children and its association with three major soil-transmitted helminths (STH) in rural areas of southern China. The study also aims to determine risk factors for stunting and to provide guidance on the prevention and control of stunting and STH infections for future studies in this field.
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Aetiological factors contributing to the development of primary laryngeal aspergillosis in immunocompetent patients.
J. Med. Microbiol.
PUBLISHED: 06-24-2010
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Primary laryngeal aspergillosis is extremely rare, especially in an immunocompetent host. It is commonly found as part of a systemic infection involving the respiratory system in immunocompromised people. Two cases of laryngeal aspergillosis with no systemic extension and no generalized immune deficiency are presented here. We report what is to the best of our knowledge only the second case of Aspergillus infection in a vocal cord cyst. Aspergillus species were identified in tissue sections and confirmed by PCR studies. We present a literature review of laryngeal aspergillosis cases and discuss predisposing factors, clinical presentation, histopathology, PCR, diagnosis and treatment of Aspergillus laryngitis. The known aetiological causes of the disease are increasing and include iatrogenic factors, vocal abuse, vocal-fold cysts and occupational factors, and immunocompetent patients are susceptible to these predisposing factors.
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[Clinical analysis and screening for SCN1A gene mutation in two pedigrees of generalized epilepsies with febrile seizures plus].
Zhonghua Er Ke Za Zhi
PUBLISHED: 12-03-2009
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To study the clinical and genetic characteristics of generalized epilepsy with febrile seizures plus (GEFS).
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[GIS/RS/GPS application in the study of relationship between natural factors and malaria transmission].
Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi
PUBLISHED: 10-27-2009
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Malaria transmission is highly related to the natural factors such as temperature, rainfall and topography. Recently, the GIS/RS/GPS techniques were developed based on computer science and spatial analysis technology, which were widely used in data collection and analysis, establishment of mathematics model. This paper reviews the applications and development of GIS/RS/GPS techniques in the studies of relationship between natural factors and malaria transmission.
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Repair of acquired posterior choanal stenosis and atresia by temperature-controlled radio frequency with the aid of an endoscope.
Arch. Otolaryngol. Head Neck Surg.
PUBLISHED: 05-20-2009
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To examine the clinical effects of temperature-controlled radio frequency (TCRF) repair of acquired choanal stenosis and atresia with the aid of an endoscope and to discuss the value of acquired choanal stenosis and atresia typing in clinical therapy.
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Inhibition of cell proliferation and glucose uptake in human laryngeal carcinoma cells by antisense oligonucleotides against glucose transporter-1.
Head Neck
PUBLISHED: 05-15-2009
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Malignant cells show increased glucose uptake in vitro and in vivo, which is thought to be mediated by glucose transporters. In this study, we investigated the effect of plasmid-derived antisense RNA against the Glut-l gene on proliferation and glucose uptake in laryngeal carcinoma Hep-2 cells.
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Changing trends in the clinical features of laryngeal tuberculosis: a report of 19 cases.
Int. J. Infect. Dis.
PUBLISHED: 03-25-2009
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This study was undertaken to evaluate the changing trends in the clinical features of laryngeal tuberculosis, with the goal of reducing misdiagnosis and incorrect treatment.
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Anopheles pseudowillmori is the predominant malaria vector in Motuo County, Tibet Autonomous Region.
Malar. J.
PUBLISHED: 03-16-2009
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Malaria is endemic in Linzhi Prefecture in the Tibet Autonomous Region (TAR), but the vector for malaria transmission had never been identified.
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[Study on malaria vectors in malaria endemic areas of Tibet autonomous region].
Zhongguo Xue Xi Chong Bing Fang Zhi Za Zhi
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The malaria situation in Tibet has been in an active status and the malaria incidence reached the second in China in 2010. Malaria vector prevention and control is one of the important methods for malaria control, while the malaria vectors are still unknown in Tibet. The author summarized the past researches on malaria vectors in Tibet, so as to provide the evidence for improving malaria control investigation in malaria endemic areas of Tibet, with hopes to provide useful vector message for other researcher.
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[Malaria situation in the Peoples Republic of China in 2011].
Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi
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Totally 4 479 malaria cases were reported through the annual reporting system from 782 counties of 27 Provinces/ Municipalities/Autonomous Regions (P/M/A) in 2011, this created a new-low number representing 43.0% reduction compared with 7 855 cases in 2010, and accordingly the annual incidence was reduced to 0.033 4/10 000. However, the number of malaria deaths increased to 33 from 19 in 2010. Among the 782 counties with reported cases, 2 counties of Motuo (16.466 0/10 000) in Tibet and Ruili (12.235 2/ 10000) in Yunnan had an incidence of more than 10/10 000, 10 counties in Yunnan and 1 county in Guizhou had an incidence between 1/10 000 and 10/10 000, and that of the others was below 1/10 000. The malaria areas and transmission got further confined than in 2010. Out of the 4 479 malaria cases, a proportion of 29.3% was reported as the indigenous cases who mainly distributed in the provinces of Anhui (40.0%), Yunnan (25.8%), Henan (12.6%), Guizhou (10.4%) and Hubei (6.1%), a proportion of 66.4% was reported as the abroad-imported cases who mainly distributed in Yunnan (36.5%), Jiangsu (12.0%), Henan (6.2%), Sichuan (5.8%) and Hunan (4.8%), and the remaining 4.3% were domestically-mobile cases. Meanwhile, the confirmed cases took 81.7% while the other 18.3% were clinically diagnosed cases. Among the confirmed cases, 56.7% were Plasmodium vivax cases reported from 25 provinces, 40.2% were P. falciparum cases reported from 22 provinces, 1.1% were mixed infections of P. vivas and P. falciparum reported from 10 provinces, and the remaining 1.9% were P. malariae or P. ovale cases reported from 14 provinces. The 32 indigenous falciparum malaria cases were found only in Yunnan Province. Yunnan was still the major malaria province which ranked No.1 in the country in terms of the case number, 1 522 cases representing 42.4% decrease of the last year with an incidence of 0.331 4/10 000. Among the cases, 301 were falciparum malaria accounting for 20.3% of the national falciparum malaria figure. As previous key malaria province however, Hainan reported only 9 malaria cases indicating 88.5% decrease of the last year with an incidence of 0.010 4/ 10 000, and ranked down to No. 25. In central China, another major malaria region, the case number decreased considerably. However, Anhui still reported 644 malaria cases though with a decrease of 65.5% in comparison to that of 2010, ranked No. 2, accounting for 14.4% of the countrys malaria cases with an incidence of 0.100 0/10 000. In Jiangsu, 374 cases were reported and decreased by 3.1% with an incidence of 0.050 8/10 000. In Henan, the number of reported cases was 358 and decreased by 59.9% with an incidence of 0.038 7/10 000. In Hubei, 167 malaria cases were reported and decreased by 61.1% with an incidence of 0.027 7/10 000. Cases reported from other provinces occupied 31.4% of the total. Respectively from Guizhou, Sichuan, Guangxi, Guangdong, Zhejiang, Hunan and Shandong provinces, 100-200 malaria cases were reported; and the number of cases was less than 100 in the provinces of Fujian, Chongqing, Shanghai, Hebei, Beijing, Tianjin, Xinjiang, Ningxia, Jiangxi, Liaoning, Shaanxi, Shanxi, Gansu and Tibet. Although a delightful progress was made with a substantial reduction of malaria transmission, China had to face the following challenges relating to malaria endemic situation: 1) Malaria cases appeared in almost one thousand counties in most provinces and nearly 200 counties reported indigenous cases. In some counties the transmission is still high, and it is still critical for the provinces of Anhui, Yunnan, Henan, Guizhou, Hubei and Tibet to interrupt the local malaria transmission. 2) Imported malaria cases were dominant and widely distributed in the country including the 4 plasmodium species, this could bring about high risks of re-introduction of malaria transmission in areas where malaria was effectively under control, particularly could cause more malaria deaths in the circumstances of increased imported falciparum malaria cases, therefore provinces with more mobile population and imported cases need to pay higher attention. 3) A number of clinically diagnosed but unconfirmed cases still exist in malaria elimination stage which need to be addressed through strengthening local laboratory diagnosis capabilities.
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Correlation of computed tomography with pathological features in angiomatous nasal polyps.
PLoS ONE
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Angiomatous nasal polyps (ANPs), also known as angiectatic polyps, have rarely been reported in the literature. ANPs are characterized by extensive vascular proliferation and ectasia. ANPs can grow rapidly and exhibit aggressive clinical behavior that could simulate malignancy preoperatively, and they are easily confused with other diseases. In the present study, we analyzed the correlation between the computed tomography (CT) findings of nasal angiomatous polyps and their pathological features.
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Expression and significance of hypoxia-inducible factor-1? and glucose transporter-1 in laryngeal carcinoma.
Oncol Lett
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Malignant cells show increased glucose uptake in vitro and in vivo. This process is considered to be mediated by glucose transporters (GLUTs). Hypoxia-inducible factor-1? (HIF-1?) may upregulate GLUT-1 expression. Little is known about the correlation between HIF-1? and GLUT-1 expression in laryngeal carcinoma. The current study investigated this correlation immunohistochemically, according to various clinical and pathological features, in 49 paraffin-embedded archival tissue blocks from patients with laryngeal squamous cell carcinoma. HIF-1? and GLUT-1 expression was detected in 63.3 (31/49) and 55.1% (27/49) of the tumour samples, respectively. HIF-1? expression was significantly correlated with lymph node classification (P=0.018), recurrence (P=0.02) and metastasis (P=0.031). GLUT-1A expression was significantly associated with recurrence (P=0.02) and metastasis (P=0.01). Univariate analyses revealed that HIF-1? (?(2)=8.2; P=0.004) and GLUT-1 expression (?(2)=9.0; P=0.003) were significantly associated with a poorer survival rate. In a multivariate analysis, GLUT-1 expression (P=0.006) was a significant predictor of poor survival rate, as well as the primary tumour site, lymph node invasion and distant metastasis. Based on Spearmans analysis, GLUT-1 expression and phosphatidylinositol 3-kinase (PI3K) expression were significantly correlated (r=0.504; P=0.000). This is the first study to demonstrate a significant correlation between GLUT-1 and HIF-1? expression in laryngeal carcinoma and to show increased GLUT-1 expression as an independent survival rate predictor. These results suggest that GLUT-1 is a potential new therapeutic target for laryngeal carcinoma.
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[Surveillance and forecast of schistosomiasis based on sentinel mouse technique in key water regions of Wuhan City in 2011].
Zhongguo Xue Xi Chong Bing Fang Zhi Za Zhi
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To understand the water infectivity of schistosome in key water regions of Wuhan City and explore the role of a sentinel mouse technique on surveillance and forecast system for schistosomiasis.
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[Research on ginsenosides composition in wild ginseng leaves in different growing years].
Zhongguo Zhong Yao Za Zhi
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To analyze ginsenosides composition in wild ginseng leaves with different growth years.
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Recurrent prognostic factors and expression of GLUT-1, PI3K and p-Akt in adenoid cystic carcinomas of the head and neck: Clinicopathological features and biomarkers of adenoid cystic carcinoma.
Oncol Lett
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The purpose of this study was to explore the factors associated with the recurrence of adenoid cystic carcinomas (ACCs). We examined the recurrence values of clinicopathological variables and GLUT-1, p-Akt and PI3K expression in 42 patients with ACC. Of the 42 patients, 17 developed recurrence following initial surgery. The positive rates of GLUT-1, PI3K and p-Akt protein expression in ACC were 38.1, 38.1 and 50.0%, respectively. The expression of GLUT-1, p-Akt or PI3K protein in ACC was higher than that in inflammatory lesions or benign tumors. Our study demonstrated that T stage, a positive resection margin, perineural invasion, surgery without postoperative radiotherapy and the expression of GLUT-1, PI3K and p-Akt were factors predictive of recurrence by univariate analyses. In multivariate analyses, perineural invasion, a positive resection margin and p-Akt were significant predictors of recurrence. Initial surgery is very significant in the recurrence of ACC. Overexpression of GLUT-1, PI3K and p-Akt may also play a role in its development and recurrence.
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Effect of Tong Qiao drops on the expression of eotaxin, IL-13 in the nasal mucosa of rats with allergic rhinitis.
J Chin Med Assoc
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In recent years, particularly in China, many Chinese medicines and prescriptions for treating allergic rhinitis have been evaluated for their clinical relevance. Studies have found that numerous herbs and their constituent compounds can significantly alleviate allergic symptoms and are effective treatments for allergic rhinitis. The purpose of this study was to examine the modulatory effect of Tong Qiao nose drops on allergy symptoms and the expression of cytokines in the nasal mucosa of rats with ovalbumin-induced allergic rhinitis.
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[Report of analysis of National Technique Competition for Diagnosis of Parasitic Diseases in 2011--II Analysis of capabilities of Plasmodium detection].
Zhongguo Xue Xi Chong Bing Fang Zhi Za Zhi
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To analyze the results of the National Technique Competition for Diagnosis of Parasitic Diseases in 2011, so as to understand the capability of detection on Plasmodium among professionals from institutes for disease control and prevention at different levels.
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What is Visualize?

JoVE Visualize is a tool created to match the last 5 years of PubMed publications to methods in JoVE's video library.

How does it work?

We use abstracts found on PubMed and match them to JoVE videos to create a list of 10 to 30 related methods videos.

Video X seems to be unrelated to Abstract Y...

In developing our video relationships, we compare around 5 million PubMed articles to our library of over 4,500 methods videos. In some cases the language used in the PubMed abstracts makes matching that content to a JoVE video difficult. In other cases, there happens not to be any content in our video library that is relevant to the topic of a given abstract. In these cases, our algorithms are trying their best to display videos with relevant content, which can sometimes result in matched videos with only a slight relation.