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Find video protocols related to scientific articles indexed in Pubmed.
Autologous fat graft and bone marrow-derived mesenchymal stem cells assisted fat graft for treatment of Parry-Romberg syndrome.
Ann Plast Surg
PUBLISHED: 07-09-2014
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Progressive facial hemiatrophy, also called Parry-Romberg syndrome (PRS), is characterized by slowly progressive atrophy of one side of the face and primarily involves the subcutaneous tissue and fat. The restoration of facial contour and symmetry in patients affected by PRS still remains a challenge clinically. Fat graft is a promising treatment but has some shortcomings, such as unpredictability and low rate of graft survival due to partial necrosis. To obviate these disadvantages, fat graft assisted by bone marrow-derived mesenchymal stem cells (BMSCs) was used to treat PRS patients and the outcome was evaluated in comparison with the conventional treatment by autologous fat graft.
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[An alternative model of composite tissue transplantation in rat: the femur osteomyocutaneous flap].
Zhonghua Zheng Xing Wai Ke Za Zhi
PUBLISHED: 04-24-2014
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To reconstruct a simpler and reliable composite tissue transplantation model-the femur osteomyocutaneous flap for the replacement of hindlimb transplantation.
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[Vacuum sealing drainage promotes experimental pig explosive abdomen wound healing].
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi
PUBLISHED: 03-11-2014
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To explore the roles of vacuum sealing drainage (VSD) in controlling infection and promoting healing on the experimental pigs with blast injury in the abdomen and exposed internal organs.
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Enhancement of fat graft survival by bone marrow-derived mesenchymal stem cell therapy.
Plast. Reconstr. Surg.
PUBLISHED: 10-30-2013
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Adipose-derived stem cells can improve fat graft survival, but there is no literature reporting whether bone marrow-derived mesenchymal stem cells enhance fat graft survival. The authors explored the feasibility of enhancing fat graft survival using bone marrow-derived mesenchymal stem cells.
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Morphological Features of Cell Death and Tissue Remolding of Fat Grafts.
Ann Plast Surg
PUBLISHED: 10-24-2013
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Fat tissue graft has been commonly used for soft tissue augmentation. However, the mechanisms underlying the maintenance of graft volume and weight are still unclear. As morphological features provide direct evidences for cell death and survival, we aimed to investigate the fate of grafted adipocytes and the dynamic changes in the remodeling of adipose tissues by transmission electron microscopy technique.
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[Application of computed tomographic angiography in repairing skin defect after scalp avulsion with free latissimus dorsi flap transplantation].
Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi
PUBLISHED: 05-16-2013
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To investigate the clinical value of computed tomographic angiography (CTA) and three-dimensional reconstruction technique in repairing scalp avulsion wound with large skull exposure by the free latissimus dorsi flap transplantation.
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The utilization of perforated bioinert chambers to generate an in vivo isolated space for tissue engineering involving chondrocytes, mesenchymal stem cells, and fibroblasts.
Tissue Eng Part C Methods
PUBLISHED: 01-31-2013
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Vascular invasion and admixture of the nude mouse cells with seeded cells make it difficult to reapply the regenerated tissues to the restoration of host tissue defects. Therefore, a device that is capable of allowing for autologous or allogenic tissue growth while preventing host tissue invasion will be a valuable tool for in vivo tissue engineering. We have previously fabricated a novel silicon-perforated chamber. The aim of this study was to evaluate whether this chamber, after being implanted subcutaneously in experimental animals, would hinder host tissue ingrowth while providing an environment inside its cavity for in vivo growth of either autologous or allogenic implant cells. We found that the chamber did not induce severe foreign body reaction, and the chambers with perforated pores of 1-3 mm in diameter effectively inhibited the host granulation tissue or vascular invasion for as long as 3 months. In addition, the exudates rich in vascular endothelial growth factor, basic fibroblast growth factor, transforming growth factor-?, insulin-like growth factor-1, and platelet-derived growth factor-BB were steadily generated and collected in the chambers. In vitro cell culture studies revealed that the exudates were able to support the viability and proliferation of rabbit chondrocytes, rat mesenchymal stem cells, and human fibroblasts. The results indicate that this novel chamber could potentially provide an environment favorable for in vivo tissue engineering while effectively preventing host tissue or vascular invasion.
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Repair of facial scars by the free expanded deltopectoral flap.
Plast. Reconstr. Surg.
PUBLISHED: 01-30-2013
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Facial scars caused by burns and trauma severely affect patients function and appearance. The expanded deltopectoral flap to local shift is the traditional method of repair, but for patients with the compulsive position after pedicle transfer, the therapeutic outcome is not satisfactory. The authors explored the surgical procedures for repairing facial scars by using the free expanded deltopectoral flap, which can reduce patient suffering and ensure a satisfactory outcome.
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[Research progress in immune of composite tissue allotransplantation].
Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi
PUBLISHED: 11-11-2011
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To introduce the research progress in the immune of composite tissue allotransplantation.
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Use of autologous chondrocytes and bioinert perforated chambers to tissue engineer cartilage in vivo.
J. Surg. Res.
PUBLISHED: 06-09-2011
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To explore the potential applications of a chamber for in vivo tissue engineering, and to establish a novel model for in vivo tissue-engineered cartilage.
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Ex vivo transfer of adenovirus-mediated CTLA4Ig gene combined with a short course of rapamycin therapy prolongs free flap allograft survival.
Plast. Reconstr. Surg.
PUBLISHED: 05-03-2011
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Adenovirus-mediated cytotoxic T-lymphocyte-associated antigen-4 immunoglobulin (AdCTLA4Ig) gene transfer has been reported to enhance both organ and composite tissue grafts survival in rodent transplantation models. The authors tested the efficacy of local expression of CTLA4Ig gene on the survival of rat free flap allografts.
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[Progress of tissue engineering research in vascularized tissue engineering chamber in vivo].
Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi
PUBLISHED: 03-25-2011
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To introduce a new method of tissue engineering research by transplanting vessels to tissue engineering chamber (vascularized tissue engineering chamber) in vivo, and to review the progress of research in vascularized tissue engineering chamber.
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A smart pH responsive graphene/polyacrylamide complex via noncovalent interaction.
Nanotechnology
PUBLISHED: 07-26-2010
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We report that the graphene sheets can be stably dispersed in water by hydrophobic interaction with polyacrylamide. Most interestingly, the resultant graphene-polyacrylamide complexes show a reversible pH responsive property although polyacrylamide itself does not possess such characteristics. This method opens up novel opportunities for the potential applications of graphene in intelligent sensors, biology, medicine, nanoelectronics and other relevant areas.
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Prolonged cold ischemic time results in increased acute rejection in a rat allotransplantation model.
J. Surg. Res.
PUBLISHED: 06-13-2010
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The cold ischemic time may be more prolonged for facial tissue allografts than for organ allografts. Previous researches have shown that prolonged ischemia resulted in increased signs of rejection in a rat groin allotransplantation model; however, the relationship between cold ischemia and alloantigen-induced rejection was unclear.
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Proteomic profiling of human bone marrow mesenchymal stem cells under shear stress.
Mol. Cell. Biochem.
PUBLISHED: 02-26-2010
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Mesenchymal stem cells (MSCs) are promising seed cells for tissue engineering of blood vessels. As seed cells, MSCs must endure blood fluid shear stress after transplantation. It has been shown that fluid shear stress can regulate the proliferation and differentiation of MSCs. However, the effects of fluid shear stress on MSCs including the types of proteins modulated are still not well understood. In this study, we exposed human mesenchymal stem cells (HMSCs) to 3 dyn/cm(2) shear stress for 6 h and compared them to a control group using proteomic analysis. Thirteen specific proteins were affected by shear stress, 10 of which were up-regulated. Shear stress especially induced sustained increases in the expression of Annexin A2 and GAPDH, which have been specifically shown to affect HMSCs function. We present here the first comparative proteome analysis of effect of shear stress on HMSCs.
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Heterogeneous ultrathin films of poly(vinyl alcohol)/layered double hydroxide and montmorillonite nanosheets via layer-by-layer assembly.
J Phys Chem B
PUBLISHED: 10-29-2009
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In this paper, Co-Al layered double hydroxide (LDH) and montmorillonite (MMT) have been exfoliated into charged single layers in the solvent of formamide and water, respectively. The structures of individual layers of LDH and MMT were characterized by X-ray diffraction (XRD) and atomic force microscopy (AFM). The delamination mechanisms of LDH and MMT were also discussed. Furthermore, heterogeneous ultrathin films of poly(vinyl alcohol) (PVA)/charged inorganic nanosheets, (PVA/MMT/PVA/LDH)(n), were fabricated by layer-by-layer (LBL) assembly via hydrogen bonding. The LBL assembly process was monitored by UV-vis spectroscopy, and the structures of the heterogeneous ultrathin films were analyzed by XRD.
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Adenovirus-mediated METH1 gene expression inhibits hypertrophic scarring in a rabbit ear model.
Wound Repair Regen
PUBLISHED: 07-21-2009
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Hypertrophic scarring remains a major problem for patients who have suffered from surgeries or burns. Vascularization plays an important role in the early phase of hypertrophic scarring. Therefore, the inhibition of angiogenesis might be used as a preventive strategy. In this study, we assessed the effect of anti-angiogenesis resulting from adenovirus-mediated METH1 (metalloprotease and thrombospondin1) gene expression on the hypertrophic scar formation in a rabbit ear model of hypertrophic scarring. We first investigated the number of microvessel and microcirculatory perfusion in untreated scars on days 10, 30, 60, and 90 after epithelialization. Then, we examined the effect of anti-angiogenesis by adenovirus-mediated METH1 expression on hypertrophic scar formation by calculating the scar elevation index, counting the microvessel and argyrophilic nucleolar organizer region particle, and detecting the amount of collagen on days 30 and 60 after treatment. We found that untreated scar tissues at the proliferative phase (days 10-60 after epithelialization) had a significantly higher density of microvessel and microcirculatory perfusion than those at the mature phase (day 90 after epithelization) (both p<0.05). On days 30 and 60 after treatment, the hypertrophic scar formation was significantly inhibited in the treatment group. There was significantly reduced scar elevation index, microvessel count, number of argyrophilic nucleolar organizer region, and total collagen content for treated scars. Our results demonstrate that METH1 has a markedly inhibitive effect on the formation of hypertrophic scar, and may thus have a promising application in the prevention of human hyperthropic scars.
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An anatomical study with clinical application of one branch of the supraclavicular artery.
Clin Anat
PUBLISHED: 05-29-2009
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The supraclavicular flap is an important method for the reconstruction of the neck. In this study, the authors attempted to clarify the mechanism of blood flow into the supraclavicular flap based on the thoracic branch of the supraclavicular artery. Additionally, the authors discuss the clinical application of such anatomy. Thirty fresh cadavers and 13 preserved cadavers were dissected to observe the anatomic features of the pectorally extended supraclavicular flap. Additionally, 46 clinical cases were treated with such a flap. We identified two branches of the supraclavicular artery. A deltoid branch that extended to the acromial region and a thoracic branch that traveled to the anterior thoracic region. Using these data, all such flaps operated by us have to date, survived with satisfactory results. The pectorally extended supraclavicular flap could be used to repair defects on the ipsilateral or contralateral face, neck, and anterior thorax.
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[Advantages and disadvantages of composite tissue allotransplantation].
Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi
PUBLISHED: 04-02-2009
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To review the research progress of composite tissue allotransplantation (CTA), analyze the superiority and the inferiority, and inform the possible direction of further research.
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Modulation of Neuronal Survival Factor MEF2 by Kinases in Parkinsons Disease.
Front Physiol
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Parkinsons disease (PD) is the second most common neurodegenerative disorder due to selective death of neurons in the substantia nigra pars compacta. The cause of cell death remains largely unknown. Myocyte enhancer factor 2 (MEF2) is a group of transcriptional factors required to regulate neuronal development, synaptic plasticity, as well as survival. Recent studies show that MEF2 functions are regulated in multiple subcellular organelles and suggest that dysregulation of MEF2 plays essential roles in the pathogenesis of PD. Many kinases associated with transcription, translation, protein misfolding, autophagy, and cellular energy homeostasis are involved in the neurodegenerative process. Following the first demonstration that mitogen-activated protein kinase p38 (p38 MAPK) directly phosphorylates and activates MEF2 to promote neuronal survival, several other kinase regulators of MEF2s have been identified. These include protein kinase A and extracellular signal regulated kinase 5 as positive MEF2 regulators, and cyclin-dependent kinase 5 (Cdk5) and glycogen synthase kinase 3? as negative regulators in response to diverse toxic signals relevant to PD. It is clear that MEF2 has emerged as a key point where survival and death signals converge to exert their regulatory effects, and dysregulation of MEF2 function in multiple subcellular organelles may underlie PD pathogenesis. Moreover, several other kinases such as leucine-rich repeat kinase 2 and PTEN-induced putative kinase 1 (PINK1) are of particular interest due to their potential interaction with MEF2.
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Observations on the survival and neovascularization of fat grafts interchanged between C57BL/6-gfp and C57BL/6 mice.
Plast. Reconstr. Surg.
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Autologous fat transplantation has become a prevalent option for soft-tissue augmentation throughout the body. However, there is still much controversy over whether the fat grafts have survived or have been replaced in the recipient sites and over how the vessels grow.
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What is Visualize?

JoVE Visualize is a tool created to match the last 5 years of PubMed publications to methods in JoVE's video library.

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We use abstracts found on PubMed and match them to JoVE videos to create a list of 10 to 30 related methods videos.

Video X seems to be unrelated to Abstract Y...

In developing our video relationships, we compare around 5 million PubMed articles to our library of over 4,500 methods videos. In some cases the language used in the PubMed abstracts makes matching that content to a JoVE video difficult. In other cases, there happens not to be any content in our video library that is relevant to the topic of a given abstract. In these cases, our algorithms are trying their best to display videos with relevant content, which can sometimes result in matched videos with only a slight relation.