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Characterization by electrochemical and X-ray photoelectron spectroscopic measurements and quantum chemical calculations of N-containing functional groups introduced onto glassy carbon electrode surfaces by electrooxidation of a carbamate salt in aqueous solutions.
Langmuir
PUBLISHED: 05-01-2014
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The present paper deals with characterization of an aminated glassy carbon electrode (GCE) surface obtained by electrooxidation of ammonium carbamate in its aqueous solution (amination reaction) using electrochemical and XPS methods. From the XPS analysis, it was found that not only the primary amine group (i.e., aniline-like aromatic amine moiety) but also other N-containing functional groups (i.e., the secondary amine-like moieties containing pyrrole-type nitrogen and quaternary amine-like moieties containing graphitic quaternary nitrogen) are introduced onto the GCE surface during the amination reaction. Moreover, the presence of the primary and secondary amine groups was ascertained based on the difference in the reactivity of a Michael reaction-type addition reaction of amine groups introduced onto the GCE surface with quinone compounds having a carbonyl group and a C?C double bond (i.e., in this case, 1,2-benzoquinone which is in situ prepared by the electrooxidation of catechol) and on the electrochemical redox response of the introduced benzoquinones. This electrochemical treatment of aminated GCE with catechol led to catechol-grafted aminated GCE which indicated two surface redox couples (i.e., the Ia/Ic and IIa/IIc couples with formal potentials of E(0)'(Ia/Ic) = ca. 0.17 V and E(0)'(IIa/IIc) = ca. 0.03 V vs Ag|AgCl|KCl(sat.) in phosphate buffer solution (pH 7)). From the electrochemical behavior of catechols grafted onto the maleimide-treated aminated GCE and on the methylamine-treated GCE, it was found that the catechol associated with the primary amine groups gave the IIa/IIc redox peaks, while the catechol bound to the secondary amine groups gave the Ia/Ic redox peaks. Further electrochemical measurements and quantum chemical calculations concluded that the IIa/IIc redox peaks are ascribed to the surface-redox reaction of the 1,2-dihydroxybenzene/1,2-benzoquinone couple, while those of the 1,2-dihydroxybenzene/1,2-benzoquinone and the N-(4'-hydroxyphenyl)-p-aminophenol/indophenol couples can be associated with the Ia/Ic redox peaks.
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Electroless deposition of platinum nanoparticles in room-temperature ionic liquids.
Langmuir
PUBLISHED: 09-13-2013
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The electroless deposition of Pt nanoparticles (Pt-NPs) could be carried out by dissolving potassium tetrachloroplatinate(II) (K2[PtCl4]) in 1-ethyl-3-methylimidazolium (EMI(+)) room-temperature ionic liquids (RTILs) containing bis(trifluoromethylsulfonyl) imide (NTf2(-)) or tetrafluoroborate (BF4(-)) anion and small cations, such as H(+), K(+), and Li(+). In this case, no deposition of Pt-NPs occurred in RTILs without such small cations. The formation of Pt-NPs was only observed in RTILs containing trifluoromethanesulfonimide (HNTf2) and protons at high temperature (?80 °C) when potassium hexachloroplatinate(IV) (K2[PtCl6]) was dissolved in the RTILs. The obtained Pt-NPs gave a characteristic absorption spectrum of ultrasmall Pt-NPs. The ultrasmall and uniform Pt-NPs of ca. 1-4 nm in diameter were produced and the Pt-NPs/EMI(+)NTf2(-) dispersion was kept stably for several months without adding any additional stabilizers or capping molecules. The identified Fourier-transform patterns along the [0 1 1] zone axis were observed for the TEM images of Pt-NPs. On the basis of the results obtained, a probable mechanism of the electroless formation of Pt-NPs is discussed.
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Direct electrochemistry and intramolecular electron transfer of ascorbate oxidase confined on l-cysteine self-assembled gold electrode.
Bioelectrochemistry
PUBLISHED: 04-23-2013
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A direct electrochemistry and intramolecular electron transfer of multicopper oxidases are of a great importance for the fabrication of these enzyme-based bioelectrochemical-devices. Ascorbate oxidase from Acremonium sp. (ASOM) has been successfully immobilized via a chemisorptive interaction on the l-cysteine self-assembled monolayer modified gold electrode (cys-SAM/AuE). Thermodynamics and kinetics of adsorption of ASOM on the cys-SAM/AuE were studied using cyclic voltammetry. A well-defined redox wave centered at 166±3mV (vs. Ag?AgCl?KCl(sat.)) was observed in 5.0mM phosphate buffer solution (pH7.0) at the fabricated ASOM electrode, abbreviated as ASOM/cys-SAM/AuE, confirming a direct electrochemistry, i.e., a direct electron transfer (DET) between ASOM and cys-SAM/AuE. The direct electrochemistry of ASOM was further confirmed by taking into account the chemical oxidation of ascorbic acid (AA) by O2 via an intramolecular electron transfer in the ASOM as well as the electrocatalytic oxidation of AA at the ASOM/cys-SAM/AuE. Thermodynamics and kinetics of the adsorption of ASOM on the cys-SAM/AuE have been elaborated along with its direct electron transfer at the modified electrodes on the basis of its intramolecular electron transfer and electrocatalytic activity towards ascorbic acid oxidation and O2 reduction. ASOM saturated surface area was obtained as 2.41×10(-11)molcm(-2) with the apparent adsorption coefficient of 1.63×10(6)Lmol(-1). The ASOM confined on the cys-SAM/AuE possesses its essential enzymatic function.
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Electrodeposition of platinum nanoparticles in a room-temperature ionic liquid.
Langmuir
PUBLISHED: 11-08-2011
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The electrochemistry of the [PtCl(6)](2-)-[PtCl(4)](2-)-Pt redox system on a glassy carbon (GC) electrode in a room-temperature ionic liquid (RTIL) [i.e., N,N-diethyl-N-methyl-N-(2-methoxyethyl)ammonium tetrafluoroborate (DEMEBF(4))] has been examined. The two-step four-electron reduction of [PtCl(6)](2-) to Pt, i.e., reduction of [PtCl(6)](2-) to [PtCl(4)](2-) and further reduction of [PtCl(4)](2-) to Pt, occurs separately in this RTIL in contrast to the one-step four-electron reduction of [PtCl(6)](2-) to Pt in aqueous media. The cathodic and anodic peaks corresponding to the [PtCl(6)](2-)/[PtCl(4)](2-) redox couple were observed at ca. -1.1 and 0.6 V vs a Pt wire quasi-reference electrode, respectively, while those observed at -2.8 and -0.5 V were found to correspond to the [PtCl(4)](2-)/Pt redox couple. The disproportionation reaction of the two-electron reduction product of [PtCl(6)](2-) (i.e., [PtCl(4)](2-)) to [PtCl(6)](2-) and Pt metal was also found to occur significantly. The electrodeposition of Pt nanoparticles could be carried out on a GC electrode in DEMEBF(4) containing [PtCl(6)](2-) by holding the potential at -3.5 or -2.0 V. At -3.5 V, the four-electron reduction of [PtCl(6)](2-) to Pt can take place, while at -2.0 V the two-electron reduction of [PtCl(6)](2-) to [PtCl(4)](2-) occurs. The results obtained demonstrate that the electrodeposition of Pt at -3.5 V may occur via a series of reductions of [PtCl(6)](2-) to [PtCl(4)](2-) and further [PtCl(4)](2-) to Pt and at -2.0 V via a disproportionation reaction of [PtCl(4)](2-) to [PtCl(6)](2-) and Pt. Furthermore, the deposition potential of Pt nanoparticles was found to largely influence their size and morphology as well as the relative ratio of Pt(110) and Pt(100) crystalline orientation domains. The sizes of the Pt nanoparticles prepared by holding the electrode potential at -2.0 and -3.5 V are almost the same, in the range of ca. 1-2 nm. These small nanoparticles are "grown" to form bigger particles with different morphologies: In the case of the deposition at -2.0 V, the GC electrode surface is totally, relatively compactly covered with Pt particles of relatively uniform size of ca. 10-50 nm. On the other hand, in the case of the electrodeposition at -3.5 V, small particles of ca. 50-100 nm and the grown-up particles of ca. 100-200 nm cover the GC surface irregularly and coarsely. Interestingly, the Pt nanoparticles prepared by holding the potential at -2.0 and -3.5 V are relatively enriched in Pt(100) and Pt(110) facets, respectively.
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Generation of gaseous sulfur-containing compounds in tumour tissue and suppression of gas diffusion as an antitumour treatment.
Gut
PUBLISHED: 08-11-2011
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The mechanisms of cancer cell growth and metastasis are still not entirely understood, especially from the viewpoint of chemical reactions in tumours. Glycolytic metabolism is markedly accelerated in cancer cells, causing the accumulation of glucose (a reducing sugar) and methionine (an amino acid), which can non-enzymatically react and form carcinogenic substances. There is speculation that this reaction produces gaseous sulfur-containing compounds in tumour tissue. The aims of this study were to clarify the products in tumour and to investigate their effect on tumour proliferation.
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pH Dependence of the size and crystallographic orientation of the gold nanoparticles prepared by seed-mediated growth.
Langmuir
PUBLISHED: 03-16-2011
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The effect of the pH of the growth solution on the size and crystallographic orientation of gold nanoparticles (GNPs) was studied during the course of the preparation of surface-confined spherical GNPs following a two-step protocol (electrochemical and chemical). GNPs were first electrodeposited onto a clean glassy carbon (GC) electrode and these GNPs were used as seeds. Seed-mediated growth of the electrodeposited GNPs was performed in a solution of H[AuCl(4)] at various pHs (5.0 to 0.5) using NH(2)OH as a reducing agent. The thus-prepared GNPs were characterized by electrochemical, UV-visible absorption spectral, SEM, and TEM studies. The nucleation (i.e., formation of the new seeds) was found to dominate over growth (i.e., enlargement of the seed particles) process at higher pH during NH(2)OH seeding, whereas only growth was recognized at low pH (0.5). Nonspherical byproducts were noticed when the seed-mediated growth was performed at higher pHs, but at pH 0.5 only spherical GNPs were observed. The present method provides a way out for the preparation of GNPs with homogeneous shape resolving the problem of simultaneous formation of nonspherical byproducts during the seed-mediated growth as well as for the preparation of GNPs with a Au(111) facet ratio as high as 97%. On the basis of the obtained results, the mechanism of the growth process at low pH is also discussed. Interestingly, an enhanced electrochemical response was obtained for the oxidation of H(2)O(2) using the GNPs prepared at pH 0.5.
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Selective detection of As(III) at the Au(111)-like polycrystalline gold electrode.
Anal. Chem.
PUBLISHED: 10-25-2010
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Selective electrochemical detection of As(III) using a highly sensitive platform based on a Au(111)-like surface is described. The Au(111)-like surface was achieved for the first time by the partial reductive desorption of n-butanethiol (n-BT) from polycrystalline gold (poly-Au), on which a self-assembled monolayer (SAM) of n-BT was formed previously, which allows the selective blockage of the Au(100) and Au(110) surface domains by n-BT while the Au(111) domain remains bare. Square wave anodic stripping voltammetry (SWASV) using the Au(111)-like poly-Au electrode confirms the successful detection of As(III) without any interference from Cu(II). The fabricated electrode is stable and highly sensitive even in the presence of Cu(II), and it shows a linear response for As(III) up to 15 ?M. The detection limit (S/N = 3) toward As(III) is 0.28 ppb, which is far below the guideline value given by World Health Organization (WHO). The electrode was applicable for the analysis of spiked arsenic in tap water containing a significant amount of various other ion elements. The results indicate that the Au(111)-like poly-Au electrode could be promising for the electrochemical detection of trace level of As(III) in real samples without any interference from Cu(II).
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Fabrication of Au(111) nanoparticle-like electrode through a seed-mediated growth.
Chem. Commun. (Camb.)
PUBLISHED: 06-11-2010
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We demonstrate a seed-mediated growth of electrodeposited gold-nanoparticles (GNPs) onto a glassy carbon (GC) electrode from a solution of H[AuCl(4)] containing NH(2)OH at pH 0.5, resulting in a Au(111) facet ratio as high as 97%.
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Determination of formal potential of NADH/NAD+ redox couple and catalytic oxidation of NADH using poly(phenosafranin)-modified carbon electrodes.
Bioelectrochemistry
PUBLISHED: 05-27-2010
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The electrochemical regeneration of NADH/NAD(+) redox couple has been studied using poly(phenosafranin) (PPS)-modified carbon electrodes to evaluate the formal potential and catalytic rate constant for the oxidation of NADH. The PPS-modified electrodes were prepared by electropolymerization of phenosafranin onto different carbon substrates (glassy carbon (GC) and basal-plane pyrolytic graphite (BPPG)) in different electrolytic solutions. The formal potential was estimated to be -0.365±0.002V vs. SHE at pH 7.0. As for the bare carbon electrodes, the oxidation of NADH at the BPPG electrode was found to be enhanced compared with the GC electrode. For the PPS-modified electrodes, it was found that the electrocatalysis of PPS-modified electrodes for the oxidation of NADH largely depends on the carbon substrate and electrolyte solution employed for their preparation, i.e., the PPS-modified BPPG electrode prepared in 0.2M NaClO(4)/acetonitrile solution exhibits an excellent and persistent electrocatalytic property toward NADH oxidation in phosphate buffer solution (pH 7.0) with a diminution of the overpotential of about 740 and 670mV compared with those at the bare GC electrode and the PPS-modified GC electrode prepared in 0.2M H(2)SO(4) solution, respectively. A quantitative analysis of the electrocatalytic reaction based on rotating disk voltammetry gave the electrocatalytic reaction rate constants of the order of 10(3)-10(4)M(-)(1)s(-1) depending on the preparation conditions of the PPS-modified electrodes.
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EQCM study of the [Au(III)Cl4](-)-[Au(I)Cl2](-)-Au(0) redox system in 1-ethyl-3-methylimidazolium tetrafluoroborate room-temperature ionic liquid.
Langmuir
PUBLISHED: 02-19-2010
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The electrochemical behavior of the [Au(III)Cl(4)](-)-[Au(I)Cl(2)](-)-Au(0) redox system in room temperature ionic liquid (RTIL) of 1-ethyl-3-methylimidazolium tetrafluoroborate (EMIBF(4)) has been investigated quantitatively using an in situ electrochemical quartz crystal microbalance (EQCM) technique based on a Pt film-coated quartz crystal electrode (Pt-QCE). A series of two-electron (2e) and one-electron (1e) reductions of the [Au(III)Cl(4)](-) to [Au(I)Cl(2)](-) and [Au(I)Cl(2)](-) to Au metal were recognized at the Pt surface. Besides, the disproportionation reaction of [Au(I)Cl(2)](-) (i.e., the 2e-reduction product of [Au(III)Cl(4)](-)) to [Au(III)Cl(4)](-) and Au metal was also observed. Electro-dissolution of the Au deposited on the Pt electrode through a 1e-oxidation reaction in the presence of chloride ions was also confirmed using the Pt-QCE based EQCM technique. A 2e-oxidation reaction of [Au(I)Cl(2)](-) (i.e., the dissolved product) to [Au(III)Cl(4)](-) along with the oxidation of Cl(-) ion on the Pt surface was also realized at high anodic potential. The results demonstrate that in situ EQCM technique is applicable and powerful in elucidating electrochemical surface phenomena accompanying a mass change in RTIL.
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Enhanced catalytic reduction of oxygen at tantalum deposited platinum electrode.
Chem. Commun. (Camb.)
PUBLISHED: 01-08-2010
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A tantalum deposited platinum electrode that offers an enhanced catalytic four-electron reduction of oxygen over the bare platinum electrode in acidic solution is explored.
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Stability of superoxide ion in imidazolium cation-based room-temperature ionic liquids.
J Phys Chem A
PUBLISHED: 01-13-2009
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The stability of superoxide ion (O(2)(*-)) generated chemically by dissolving KO(2) in dried dimethyl sulfoxide solutions containing imidazolium cation [e.g., 1-ethyl-3-methylimidazolium (EMI(+)) and 1-n-butyl-2,3-dimethylimidazolium (BMMI(+))] based ionic liquids (ILs) was investigated with UV-visible spectroscopic, NMR, and voltammetric techniques and an ab initio molecular orbital calculation. UV-visible spectroscopic and cyclic voltammetric measurements reveal that the O(2)(*-) species reacts with BMMI(+) and EMI(+) cations of ILs to form hydrogen peroxide. The pseudo first order rate constant for the reaction of BMMI(+) and O(2)(*-) species was found to be about 2.5 x 10(-3) s(-1). With a molecular orbital calculation, the O(2)(*-) species is understood to attack the 2-position (C-2) of the imidazolium ring (i.e., BMMI(+)) to form an ion pair complex in which one oxygen atom is bounded to C-2 and the other to the hydrogen atom of -CH(3) group attached to C-2. Eventually, the ion pair complex of BMMI(+) cation and O(2)(*-) species undergoes a ring opening reaction as evidenced with (1)H NMR measurement.
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