JoVE Visualize What is visualize?
Stop Reading. Start Watching.
Advanced Search
Stop Reading. Start Watching.
Regular Search
Find video protocols related to scientific articles indexed in Pubmed.
Inactivation of dinoflagellate Scripsiella trochoidea in synthetic ballast water by advanced oxidation processes.
Environ Technol
PUBLISHED: 09-30-2014
Show Abstract
Hide Abstract
Ship-borne ballast water contributes significantly to the transfer of non-indigenous species across aquatic environments. To reduce the risk of bio-invasion, ballast water should be treated before discharge. In this study, the efficiencies of several conventional and advanced oxidation processes were investigated for potential ballast water treatment, using a marine dinoflagellate species, Scripsiella trochoidea, as the indicator organism. A stable and consistent culture was obtained and treated by ultraviolet (UV) light, ozone (O3), hydrogen peroxide (H2O2), and their various combinations. UV apparently inactivated the cells after only 10?s of irradiation, but subsequently photo-reactivation of the cells was observed for all methods involving UV. O3 exhibited 100% inactivation efficiency after 5?min treatment, while H2O2 only achieved maximum 80% inactivation in the same duration. Combined methods, e.g. UV/O3 and UV/H2O2, were found to inhibit photo-reactivation and improve treatment efficiency to some degree, indicating the effectiveness of using combined treatment processes. The total residual oxidant (TRO) levels of the methods were determined, and the results indicated that UV and O3 generated the lowest and highest TRO, respectively. The synergic effect of combined processes on TRO generation was found to be insignificant, and thus UV/O3 was recommended as a potentially suitable treatment process for ballast water.
Related JoVE Video
Enhanced enzymatic hydrolysis of palm pressed fiber based on the three main components: cellulose, hemicellulose, and lignin.
Appl. Biochem. Biotechnol.
PUBLISHED: 03-03-2014
Show Abstract
Hide Abstract
The enzymatic hydrolysis of the native and the pretreated palm pressed fiber (PPF) was deeply investigated by using the enzyme cocktail ACCELLERASE 1500. Together with the spent PPF from the first hydrolysis and the further doubly-treated PPF, the proportions of three main components were determined and analyzed based on a triangle figure. The proportion (cellulose/hemicelluloses/lignin) in the spent PPF was equal to 44:23:33 and the surface morphology of the spent PPF looks very similar to the native PPF surface showing poor hydrolysis efficiency. After further double treatment, the proportion was changed evidently from the original 44:23:33 to 54:21:25 and the surface structure was significantly disrupted showing a potential to be hydrolyzed completely. Additionally, all samples were characterized by Fourier transform infrared spectroscopy and X-ray diffractogram through considerations of alkaline solution treatment, so as to understand better the nature of biomass hydrolysis, from the aspect of three biomass components.
Related JoVE Video
Adsorption behavior of activated carbon derived from pyrolusite-modified sewage sludge: equilibrium modeling, kinetic and thermodynamic studies.
Water Sci. Technol.
PUBLISHED: 11-22-2011
Show Abstract
Hide Abstract
Activated carbon was developed from sewage sludge using pyrolusite as an additive. It was demonstrated that the removal efficiency of two synthetic dyes (Tracid orange GS and Direct fast turquoise blue GL) by the produced adsorbent was up to 97.6%. The activated carbon with pyrolusite addition had 38.2% higher surface area, 43.8% larger micropore and 54.4% larger mesopore production than ordinary sludge-based activated carbons. Equilibrium adsorption isotherms and kinetics were also investigated based on dyes adsorption tests. The experimental data were analyzed by the Langmuir and Freundlich models of adsorption, and the results fitted well to the Langmuir isotherm. The kinetic data have been analyzed using pseudo-first-order, pseudo-second-order and intraparticle diffusion equation. The experimental data fitted very well with pseudo-second-order kinetic model. Activation energies for the adsorption processes ranged between 8.7 and 19.1 kJ mol 1. Thermodynamic parameters such as standard free energy (deltaG0), standard enthalpy (deltaH0) and standard entropy (deltaS0) were evaluated. The adsorption of these two dyes on the activated carbon was found to be a spontaneous and endothermic process in nature.
Related JoVE Video
Impact of a metabolic uncoupler, 2,4-dichlorophenol on minimization of activated sludge production in membrane bioreactor.
Water Sci. Technol.
PUBLISHED: 09-24-2010
Show Abstract
Hide Abstract
This study investigated the effects of 2,4-dichlorophenol on reduction of activated sludge in membrane bioreactors. Significant inhibition on sludge growth and slight reduction in COD removal were observed at higher 2,4-dichlorophenol dosages. The deviation between relative specific COD removal rate (q/q0) and relative specific growth rate (?/?0) suggested that a minimum 2,4-dichlorophenol concentration was required for uncoupling of anabolism and catabolism. With the increase of the dosage of 2,4-dichlorophenol, stepwise improvement of biomass bioactivity and the reduction in activated sludge production were achieved simultaneously. Compared with the control bioreactor, the peak distribution of floc size in the 2,4-dichlorophenol added bioreactor shifted to a range of smaller floc size. Besides, addition of 2,4-dichlorophenol caused little variation of microorganism community structure and SVI value of the sludge. After 24-hour operation, the residue 2,4-dichlorophenol concentration in the bioreactors was reduced to a negligible level.
Related JoVE Video
In-depth investigation of enzymatic hydrolysis of biomass wastes based on three major components: Cellulose, hemicellulose and lignin.
Bioresour. Technol.
PUBLISHED: 03-10-2010
Show Abstract
Hide Abstract
The artificial biomass based on three biomass components (cellulose, hemicellulose and lignin) were developed on the basis of a simplex-lattice approach. Together with a natural biomass sample, they were employed in enzymatic hydrolysis researches. Different enzyme combines of two commercial enzymes (ACCELLERASE 1500 and OPTIMASH BG) showed a potential to hydrolyze hemicellulose completely. Negligible interactions among the three components were observed, and the used enzyme ACCELLERASE 1500 was proven to be weak lignin-binding. On this basis, a multiple linear-regression equation was established for predicting the reducing sugar yield based on the component proportions in a biomass. The hemicellulose and cellulose in a biomass sample were found to have different contributions in staged hydrolysis at different time periods. Furthermore, the hydrolysis of rice straw was conducted to validate the computation approach through considerations of alkaline solution pretreatment and combined enzymes function, so as to understand better the nature of biomass hydrolysis, from the aspect of three biomass components.
Related JoVE Video
Characterization of mesoporous activated carbons prepared by pyrolysis of sewage sludge with pyrolusite.
Bioresour. Technol.
PUBLISHED: 06-02-2009
Show Abstract
Hide Abstract
Activated carbons were prepared from sewage sludge by chemical activation. Pyrolusite was added as a catalyst during activation and carbonization. The influence of the mineral addition on the properties of the activated carbons produced was evaluated. The results show that activated carbons from pyrolusite-supplemented sewage sludge had up to a 75% higher BET surface area and up to a 66% increase in mesoporosity over ordinary sludge-based activated carbons. Batch adsorption experiments applying the prepared adsorbents to synthetic dye wastewater treatment yielded adsorption data well fitted to the Langmuir isotherm. The adsorbents from pyrolusite-supplemented sludges performed better in dye removal than those without mineral addition, with the carbon from pyrolusite-augmented sludge T2 presenting a significant increase in maximum adsorption capacity of 50mg/g. The properties of the adsorbents were improved during pyrolusite-catalyzed pyrolysis via enhancement of mesopore production, thus the mesopore channels may provide fast mass transfer for large molecules like dyes.
Related JoVE Video
Spectroscopic study of degradation products of ciprofloxacin, norfloxacin and lomefloxacin formed in ozonated wastewater.
Water Res.
Show Abstract
Hide Abstract
This study addressed the formation and properties of degradation products of ciprofloxacin, norfloxacin and lomefloxacin formed during ozonation of secondary wastewater effluent containing these fluoroquinolone antibiotics. The generation of the degradation products was interpreted in the context of transformations of effluent organic matter (EfOM) tracked via absorbance measurements. The structures of 20 degradation products were elucidated for ciprofloxacin and norfloxacin, respectively. 27 degradation products were identified for lomefloxacin. The prevalent oxidation pathways were suggested based on the structures of the identified products formed in the absence and presence of the hydroxyl radical scavenger t-butanol. These pathways were largely similar for all studied fluoroquinolones and involved attacks on the piperazine ring and the quinolone structure. The quinolone ring remained intact in the presence of t-butanol thus indicating that this functional group could only be oxidized by OH radicals while the piperazine ring was readily oxidized by molecular ozone. The cleavage of the quinolone moiety that resulted in several identified degradation products occurred via the attack by hydroxyl radicals on the carbon-carbon double bond adjacent to the carboxylic acid group. Lomefloxacin had more diverse oxidation products due to the presence of a methyl group on its piperazinyl ring. The concentrations of the identified degradation products behaved non-monotonically as a function of ozone dose or treatment time, yet exhibited interpretable correlations versus changes of EfOM absorbance. Examination of these correlations allowed developing a novel approach for elucidating the transformations of fluoroquinolone antibiotics during ozonation.
Related JoVE Video

What is Visualize?

JoVE Visualize is a tool created to match the last 5 years of PubMed publications to methods in JoVE's video library.

How does it work?

We use abstracts found on PubMed and match them to JoVE videos to create a list of 10 to 30 related methods videos.

Video X seems to be unrelated to Abstract Y...

In developing our video relationships, we compare around 5 million PubMed articles to our library of over 4,500 methods videos. In some cases the language used in the PubMed abstracts makes matching that content to a JoVE video difficult. In other cases, there happens not to be any content in our video library that is relevant to the topic of a given abstract. In these cases, our algorithms are trying their best to display videos with relevant content, which can sometimes result in matched videos with only a slight relation.