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Find video protocols related to scientific articles indexed in Pubmed.
Dementia among elderly in Shanghai suburb: a rural community survey.
J. Alzheimers Dis.
PUBLISHED: 10-31-2014
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The number of elderly in the world is increasing rapidly, especially in China. The prevalence of dementia among elderly was investigated in a community of Sheshan town, located in the Southwest suburb of Shanghai, China. Face-to-face interviews were conducted to collect relevant information with prepared questionnaires. The Chinese version of the Mini-Mental Status Examination was used to screen subjects with cognitive impairment (CI). Physical examinations and neuropsychological assessments were carried out. Dementia and its major subtypes, Alzheimer's disease (AD) and vascular dementia (VaD), were diagnosed by senior neurologists according to relevant diagnostic criteria. In addition, magnetic resonance imaging and EEG (with P300) were performed for a number of cases with AD or VaD. There were 1,472 participants (666 males and 806 females) aged 60 years and over in the study. A total of 167 subjects with CI were screened. Among them, dementia was recognized in 79 cases with a prevalence of 5.37% (95% confidence intervals: 4.22%-6.52%). The diagnosis of AD was made for 53 cases (16 males and 37 females) with a prevalence of 3.60% (95% confidence intervals: 2.65%-4.55%), and VaD for 21 cases (5 males and 16 females) with a prevalence of 1.43% (95% confidence intervals: 0.82%-2.03%); while the ratio of AD to VaD was 2.52. The prevalence rates of dementia among elderly from our study are higher than that previously reported from China, but in line with that reported from most world regions. A nationwide survey and surveillance system on the prevalence of dementia is recommended.
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[Ligustrazine fought against cisplatin induced ototoxicity in guinea pigs].
Zhongguo Zhong Xi Yi Jie He Za Zhi
PUBLISHED: 09-17-2014
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To study the protective effect of Ligustrazine Injection (LI) against cisplatin-induced ototoxicity and to explore its mechanism.
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[Effects of two submerged macrophytes on dissolved inorganic nitrogen in overlying water and interstitial water].
Huan Jing Ke Xue
PUBLISHED: 08-28-2014
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Ceratophyllum demersum (C. demersum) and Vallisneria spiralis L. (V. spiralis L.) were studied as model submerged macrophytes. The effects of the submerged macrophytes on the forms and concentration of the dissolved inorganic nitrogen (DIN) in the overlying water and the interstitial water, as well as the diffusion flux of DIN in the water-sediment interface were investigated by batch simulation experiment. The results indicated that the removal effect of DIN in the overlying water was better than that in the interstitial water by submerged macrophytes. The removal efficiency of DIN in the overlying water and the interstitial water followed the order of NO2(-) -N > NH4(+) -N > NO3(-) -N. The removal rate of DIN by C. demersum was higher than that of V. spiralis L. in the overlying water, while the result was converse in the interstitial water. C. demersum and V. spiralis L. decreased the diffusion flux of NH4(+) -N and NO2(-) -N, and increased the diffusion flux of NO3(-) -N significantly. Consequently, NO3(-) -N replaced NH4(+) -N and became the main form of DIN, which diffused from the interstitial water to the overlying water. The impact of the diffusion flux of NO3(-) -N between C. demersum and V. spiralis L. showed no significant difference, and the result was the same for NH4(+) -N. C. demersum and V. spiralis L. increased the width of variation of the three nitrogen forms to total DIN in the overlying water and the interstitial water, the influence on the ratio of DIN by C. demersum was greater than that of V. spiralis L. in the overlying water, while the result was opposite in the interstitial water. In general, C. demersum had more influence in the overlying water, while V. spiralis L. had more influence in the interstitial water, and the influence of DIN diffusion flux was not significant.
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Characterization of the enterovirus 71 P1 polyprotein expressed in Pichia pastor is as a candidate vaccine.
Hum Vaccin Immunother
PUBLISHED: 07-23-2014
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Human enterovirus 71 (EV71) plays an important role in hand, foot, and mouth disease (HFMD), which recently caused the death of hundreds of children in the Asia-Pacific region. However, there are no specific treatments available for EV71 infections; thus, a safe and effective vaccine is needed urgently. In this study, we developed an effective and economical method for producing EV71 polyprotein (P1 protein) in Pichia pastoris. Furthermore, we evaluated the potential of P1 protein as a candidate vaccine against EV71 virus. The data revealed that P1 protein induced persistent high cross-neutralization antibodies for different EV71 subtypes, and elicited significant splenocyte proliferation. The high levels of interleukin-10(IL-10) and interferon-gamma (IFN-?) showed that P1 protein induced Th1 and Th2 immune responses. Interestingly, vaccinating female mice with the P1 protein conferred cross-protection against different EV71 subtypes to their neonatal offspring.Compared with heat-inactivated EV71, the P1 protein elicited improved humoral and cellular immune responses and showed good cross-protection with different EV71 subtypes. Therefore, the EV71-P1 protein produced by P. pastoris is a promising candidate vaccine against EV71.
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Infection of Ustilaginoidea virens intercepts rice seed formation but activates grain-filling-related genes.
J Integr Plant Biol
PUBLISHED: 06-27-2014
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Rice false smut has become an increasingly serious disease in rice production worldwide. The typical feature of this disease is that the fungal pathogen Ustilaginoidea virens (Uv) specifically infects rice flower and forms false smut balls, the ustiloxins-containing ball-like fungal colony, of which the size is usually several times larger than that of a mature rice seed. However, the underlying mechanisms of Uv-rice interaction are poorly understood. Here, we applied time-course microscopic and transcriptional approaches to investigate rice responses to Uv infection. The results demonstrated that flower-opening process and expression of associated transcription factors, including ARF6 and ARF8, were inhibited in Uv-infected spikelets. The ovaries in infected spikelets were interrupted in fertilization and thus were unable to set seeds. However, a number of grain-filling-related genes, including seed storage protein genes, starch anabolism genes and endosperm-specific transcription factors (RISBZ1 and RPBF), were highly transcribed as if the ovaries were fertilized. In addition, critical defense-related genes like NPR1 and PR1 were down-regulated by Uv infection. Our data imply that Uv might hijack host nutrient reservoir by activation of grain-filling network for the need of growth and formation of false smut balls.
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Characterization of the enterovirus 71 VP1 protein as a vaccine candidate.
J. Med. Virol.
PUBLISHED: 06-10-2014
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Enterovirus 71 (EV71) is an important agent responsible for hand-foot-and-mouth disease (HFMD), which can cause severe neurological complications and death in children. However, there is no specific treatment for EV71 infection, and a safe and effective vaccine is needed urgently. In this study, an effective and economical method for the production of EV71-VP1 protein was developed, and the VP1 protein was evaluated in humoral and cellular immune responses as an EV71 vaccine. The results revealed that the VP1 protein induced high titers of cross-neutralizing antibodies for different EV71 subtypes, and elicited significant splenocyte proliferation. The high levels of IFN-r and IL-10 showed the VP1 protein induced a mixed Th1 and Th2 immune response. Vaccinated female mice could confer protection in their neonatal offspring. Compared with the inactivated EV71, the VP1 protein elicited similar humoral and cellular responses, but the engineered protein is safer, less expensive and can be produced more efficiently. Therefore, EV71-VP1 protein can induce effective immunologic protection against EV71 and is an ideal candidate against EV71 infection. J. Med. Virol. © 2014 Wiley Periodicals, Inc.
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5-[(2-Hy-droxy-eth-yl)(meth-yl)amino]-thio-phene-2-carbaldehyde.
Acta Crystallogr Sect E Struct Rep Online
PUBLISHED: 06-01-2014
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In the title compound, C8H11NO2S, the aldehyde group is approximately coplanar with the thio-phene ring [maximum deviation = 0.023?(2)?Å]. In the crystal, mol-ecules are linked by O-H?O hydrogen bonds into supra-molecular chains propagating along the a-axis direction.
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Acorus tatarinowii Schott extract protects PC12 cells from amyloid-beta induced neurotoxicity.
Pharmazie
PUBLISHED: 05-27-2014
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Amyloid-beta induced neurotoxicity has been identified as a major cause of Alzheimer's disease. Acorus tatarinowii Schott is one of the most frequently used Chinese herbs for Alzheimer's disease treatment. However, the effects of Acorus tatarinowii Schott on amyloid-beta mediated nerve cell damage remains unknown. In the present study, neuronal differentiated PC12 cells were used as a model to evaluate the effects of A. tatarinowii Schott extract (ATSE) against Abeta25-35 induced neurotoxicity. The results showed pretreatment with ATSE significantly protected PC12 cells from Abeta25-35 induced cell death, lactate dehydrogenase release, DNA damage, mitochondrial dysfunction and cytochrome c release from mitochondria. In addition, pretreatment with ATSE also significantly inhibited Abeta25-35 induced caspase-3 activation and reactive oxygen species generation in PC12 cells. These observations suggested that ATSE protects PC12 cells from amyloid-beta induced neurotoxicity.
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The relationship between laminoplasty opening angle and increased sagittal canal diameter and the prediction of spinal canal expansion following double-door cervical laminoplasty.
Eur Spine J
PUBLISHED: 05-14-2014
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To clarify the relationship between laminoplasty opening angle (LOA) and the increase in sagittal canal diameter (SCD) in double-door cervical laminoplasty (DDCL) and to predict the increase in SCD using the resulting formula.
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Acutissimanide, a new lignan with antioxidant activity isolated from the bark of Quercus acutissima Carruth.
Nat. Prod. Res.
PUBLISHED: 05-01-2014
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Acutissimanide (1), a new lignin, together with 11 known polyphenols (2-12) were isolated from the bark of the deciduous oak tree, Quercus acutissima Carruth. The structure of compound 1 was determined using multidimensional (1)H and (13)C NMR and mass spectroscopy. The antioxidant properties of compounds 1-12 were investigated using a 1,1-diphenyl-2-picryhydrazyl radical-scavenging assay with compounds 6-11 displaying significant antioxidant activity (EC50 values of 5.2-23.7 ?M). Our findings suggest the extracts of Q. acutissima Carruth are a potential source of natural antioxidant additives for use in the food and other allied industries.
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Synthesis of 2´-hydroxy-4´-isoprenyloxychalcone Derivatives with Potential Antidepressant-like Activity.
Med Chem
PUBLISHED: 03-26-2014
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A series of 2´-hydroxy-4´-isoprenyloxychalcone derivatives was synthesized and evaluated for its antidepressant- like activity using the FST and TST. All compounds exhibited the potential antidepressant-like activity in the FST and the TST through intraperitoneal injection. Among them, compounds 4i, 4l and 4n exhibited more potent antidepressant- like activity at a dose of 10 mg/kg. And, compounds 4i, 4l and 4n were also adequately absorbed in mice after oral administration at a dose of 30 mg/kg. In the 5-HT induced head-twitch test and yohimbine induced mortality test, compound 4i could increase the head-twitch and rise mortality in mice. The results suggested that the antidepressant effects of compound 4i may be related to via the serotonergic and noradrenergic system.
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Relationship between dioxin and steroid hormones in sera of Vietnamese men.
Biomarkers
PUBLISHED: 03-24-2014
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A recent study found an inverse correlation between serum TCDD levels and serum testosterone in the US veterans, while there is little known on the dioxin and steroid hormones about Vietnamese men. We collected blood samples from 48 men who had resided in a hotspot when exposure happened and 38 men in a non-sprayed area. Some steroid hormones levels showed significant differences between two areas. There were no correlations between steroid hormones and dioxin TEQ, after ajusting for age and other factors. Our findings indicate that steroid hormones of Vietnamese men did not correlate with dioxin TEQ in two areas.
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BMP-4 genetic variants and protein expression are associated with platinum-based chemotherapy response and prognosis in NSCLC.
Biomed Res Int
PUBLISHED: 03-19-2014
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To explore the role of genetic polymorphisms of bone morphogenic proteins 4 (BMP-4) in the response to platinum-based chemotherapy and the clinical outcome in patients with advanced nonsmall cell lung cancer (NSCLC), 938 patients with stage III (A+B) or IV NSCLC were enrolled in this study. We found that the variant genotypes of 6007C > T polymorphisms significantly associated with the chemotherapy response. The 6007CC genotype carriers had a higher chance to be responder to chemotherapy (adjusted odd ratio = 2.77; 95% CI: 1.83-4.18; adjusted < 0.001). The 6007C > T polymorphisms and BMP-4 expression also affect the prognosis of NSCLC. Patients with high BMP-4 expression had a significantly higher chance to be resistant to chemotherapy than those with low BMP-4 expression (OR = 2.81; 95% CI: 1.23-6.44; P = 0.01). The hazard ratio (HR) for 6007TT was 2.37 times higher than 6007CC (P = 0.003). In summary, the 6007C > T polymorphism of BMP-4 gene and BMP-4 tissue expression may be used as potential predictor for the chemotherapy response and prognosis of advanced NSCLC.
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Sodium arsenite induces ROS-dependent autophagic cell death in pancreatic ?-cells.
Food Chem. Toxicol.
PUBLISHED: 03-12-2014
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Inorganic arsenic is a worldwide environmental pollutant. Inorganic arsenic's positive relationship with the incidence of type 2 diabetes mellitus arouses concerns associated with its etiology in diabetes among the general human population. In this study, the inhibitor of autophagosome formation, 3-methyladenine, protected the cells against sodium arsenite cytotoxicity, and the autophagy stimulator rapamycin further decreased the cell viability of sodium arsenite-treated INS-1 cells. These finding suggested the hypothesis that autophagic cell death contributed to sodium arsenite-induced cytotoxicity in INS-1 cells. Sodium arsenite increased the autophagosome-positive puncta in INS-1 cells observed under a fluorescence microscope, and this effect was confirmed by the elevated LC3-II levels detected through Western blot. The LC3 turnover assay indicated that the accumulation of autophagosomes in the arsenite-treated INS-1 cells was due to increased formation rather than impaired degradation. The pretreatment of INS-1 cells with the ROS inhibitor NAC reduced autophagosome formation and reversed the sodium arsenite cytotoxicity, indicating that sodium arsenite-induced autophagic cell death was ROS-dependent. In summary, the precise molecular mechanisms through which arsenic is related to diabetes have not been completely elucidated, but the ROS-dependent autophagic cell death of pancreatic ?-cells described in this study may help to elucidate the underlying mechanism.
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Stabilization of multiple rib fractures in a canine model.
J. Surg. Res.
PUBLISHED: 02-14-2014
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Operative stabilization is frequently used in the clinical treatment of multiple rib fractures (MRF); however, no ideal material exists for use in this fixation. This study investigates a newly developed biodegradable plate system for the stabilization of MRF.
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Perfluorooctane sulfonate blocked autophagy flux and induced lysosome membrane permeabilization in HepG2 cells.
Food Chem. Toxicol.
PUBLISHED: 01-07-2014
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Perfluorooctane sulfonate (PFOS) is an emerging persistent organic pollutant widely distributed in the environment, wildlife and human. In this study, as observed under the transmission electron microscope, PFOS increased autophagosome numbers in HepG2 cells, and it was confirmed by elevated LC3-II levels in Western blot analysis. PFOS increased P62 level and chloroquine failed to further increase the expression of LC3-II after PFOS treatment, indicating that the accumulation of autophagosome was due to impaired degradation rather than increased formation. With acridine orange staining, we found PFOS caused lysosomal membrane permeabilization (LMP). In this study, autophasome formation inhibitor 3-methyladenine protected cells against PFOS toxicity, autophagy stimulator rapamycin further decreased cell viability and increased LDH release, cathepsin inhibitor did not influence cell viability of PFOS-treated HepG2 cells significantly. These further supported the notion that autophagic cell death contributed to PFOS-induced hepatotoxicity. In summary, the data of the present study revealed that PFOS induced LMP and consequent blockage of autophagy flux, leading to an excessive accumulation of the autophagosomes and turning autophagy into a destructive process eventually. This finding will provide clues for effective prevention and treatment of PFOS-induced hepatic disease.
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Effect of nitrogen-starvation, light intensity and iron on triacylglyceride/carbohydrate production and fatty acid profile of Neochloris oleoabundans HK-129 by a two-stage process.
Bioresour. Technol.
PUBLISHED: 01-06-2014
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Triacylglyceride (TAG) and carbohydrate are potential feedstock for biofuels production. In this study, a two-stage process was applied for enhancing TAG/carbohydrate production in the selected microalgae - Neochloris oleoabundans HK-129. In stage I, effects of nitrogen, light intensity and iron on cell growth were investigated, and the highest biomass productivity of 292.83±5.83mg/L/d was achieved. In stage II, different nitrogen-starvation periods, light intensities and iron concentrations were employed to trigger accumulation of TAG and carbohydrate. The culture under 2-day N-starvation, 200?mol/m(2)/s light intensity and 0.037mM Fe(3+) concentration produced the maximum TAG and carbohydrate productivity of 51.58mg/L/d and 90.70mg/L/d, respectively. Nitrogen starvation period and light intensity had marked effects on TAG/carbohydrate accumulation and fatty acids profile, compared to iron concentration. The microalgal lipid was mainly composed of C16/C18 fatty acids (90.02%), saturated fatty acids (29.82%), and monounsaturated fatty acids (32.67%), which is suitable for biodiesel synthesis.
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Selenium Accumulation in Unicellular Green Alga Chlorella vulgaris and Its Effects on Antioxidant Enzymes and Content of Photosynthetic Pigments.
PLoS ONE
PUBLISHED: 01-01-2014
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The aim of the present study was to investigate selenite effects in the unicellular green algae Chlorella vulgaris as a primary producer and the relationship with intracellular bioaccumulation. The effects of selenite were evaluated by measuring the effect of different selenite concentrations on algal growth during a 144 h exposure period. It was found that lower Se concentrations (?75 mg L-1) positively promoted C. vulgaris growth and acted as antioxidant by inhibiting lipid peroxidation (LPO) and intracellular reactive oxygen species (ROS). The antioxidative effect was associated with an increase in guaiacol peroxidase (GPX), catalase (CAT), superoxide dismutase (SOD) and photosynthetic pigments. Meanwhile, significant increase in the cell growth rate and organic Se content was also detected in the algae. In contrast, these changes were opposite in C. vulgaris exposed to Se higher than 100 mg L-1. The antioxidation and toxicity appeared to be correlated to Se bioaccumulation, which suggests the appropriate concentration of Se in the media accumulation of C. vulgaris should be 75 mg L-1. Taken together, C. vulgaris possesses tolerance to Se, and Se-Chlorella could be developed as antioxidative food for aquaculture and human health.
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Feasibility of ?-Sheet Breaker Peptide-H102 Treatment for Alzheimer's Disease Based on ?-Amyloid Hypothesis.
PLoS ONE
PUBLISHED: 01-01-2014
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?-amyloid hypothesis is the predominant hypothesis in the study of pathogenesis of Alzheimer's disease. This hypothesis claims that aggregation and neurotoxic effects of amyloid ? (A?) is the common pathway in a variety of etiological factors for Alzheimer's disease. A? peptide derives from amyloid precursor protein (APP). ?-sheet breaker peptides can directly prevent and reverse protein misfolding and aggregation in conformational disorders. Based on the stereochemical structure of A?1-42 and aggregation character, we had designed a series of ?-sheet breaker peptides in our previous work and screened out a 10-residue peptide ?-sheet breaker peptide, H102. We evaluated the effects of H102 on expression of P-tau, several associated proteins, inflammatory factors and apoptosis factors, and examined the cognitive ability of APP transgenic mice by behavioral test. This study aims to validate the ?-amyloid hypothesis and provide an experimental evidence for the feasibility of H102 treatment for Alzheimer's disease.
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[Clinical efficacy of tetrandrine combined with acetylcysteine effervescent tablets in treatment of silicosis].
Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi
PUBLISHED: 12-28-2013
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To observe the clinical efficacy of tetrandrine combined with acetylcysteine effervescent tablets in the treatment of silicosis.
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[Multiple scattering of visible and infrared light by sea fog over wind driving rough sea surface].
Guang Pu Xue Yu Guang Pu Fen Xi
PUBLISHED: 10-29-2013
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The present paper is concerned with computing the multiple scattering characteristics of a sea fog-sea surface couple system within this context. The single scattering characteristics of sea fog were studied by Mie theory, and the multiple scattering of sunlight by single sea fog layer was studied by radiative transfer theory. The reflection function of a statistically rough ocean surface was obtained using the standard Kirchhoff formulation, with shadowing effects taken into account. The reflection properties of the combined sea fog and ocean surface were obtained employing the adding method, and the results indicated that the reflected light intensity of sea fog increased with the sea background.
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[Clinical analysis of 25 gastric cancer cases undergoing totally laparoscopic distal gastrectomy].
Zhonghua Wei Chang Wai Ke Za Zhi
PUBLISHED: 10-26-2013
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To investigate the feasibility and safety of totally laparoscopic distal gastrectomy.
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Tamoxifen-resistant breast cancer cells possess cancer stem-like cell properties.
Chin. Med. J.
PUBLISHED: 08-29-2013
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Cancer stem cells (CSCs) are the cause of cancer recurrence because they are resistant to conventional therapy and contribute to cancer growth and metastasis. Endocrinotherapy is the most common breast cancer therapy and acquired tamoxifen (TAM) resistance is the main reason for endocrinotherapy failure during such therapy. Although acquired resistance to endocrine treatment has been extensively studied, the underlying mechanisms are unclear. We hypothesized that breast CSCs played an important role in TAM-induced resistance during breast cancer therapy. Therefore, we investigated the biological characteristics of TAM-resistant (TAM-R) breast cancer cells.
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Percutaneous radiofrequency ablation versus partial hepatectomy for small centrally located hepatocellular carcinoma.
World J Surg
PUBLISHED: 08-16-2013
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It is not known whether percutaneous radiofrequency ablation (PRFA) could get the same treatment efficacy and fewer complications as partial hepatectomy (PH) in patients with small centrally located hepatocellular carcinoma (HCC). The present study was designed to evaluate the efficacy of PH and PRFA in the treatment of small centrally located HCC.
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[The combined effects of beta-sheet breaker and hUCMSC on APP transgenic mice].
Zhongguo Ying Yong Sheng Li Xue Za Zhi
PUBLISHED: 08-15-2013
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To study the effect of combining the injection of beta-sheet breaker H102 with human umbilical cord mesenchymal stem cell (hUCMSC) on APP transgenic mice behavior, P-tau, apoptosis and the expression of relevant enzymes in the brain.
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[Prevalence and risk factors of atherosclerotic renal artery stenosis].
Zhonghua Yi Xue Za Zhi
PUBLISHED: 07-18-2013
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To explore the prevalence and risk factors of atherosclerotic renal artery stenosis (ARAS) in patients undergoing coronary angiography.
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Structure-based ensemble-QSAR model: a novel approach to the study of the EGFR tyrosine kinase and its inhibitors.
Acta Pharmacol. Sin.
PUBLISHED: 07-03-2013
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Aim:To develop a novel 3D-QSAR approach for study of the epidermal growth factor receptor tyrosine kinase (EGFR TK) and its inhibitors.Methods:One hundred thirty nine EGFR TK inhibitors were classified into 3 clusters. Ensemble docking of these inhibitors with 19 EGFR TK crystal structures was performed. Three protein structures that showed the best recognition of each cluster were selected based on the docking results. Then, a novel QSAR (ensemble-QSAR) building method was developed based on the ligand conformations determined by the corresponding protein structures.Results:Compared with the 3D-QSAR model, in which the ligand conformations were determined by a single protein structure, ensemble-QSAR exhibited higher R2 (0.87) and Q2 (0.78) values and thus appeared to be a more reliable and better predictive model. Ensemble-QSAR was also able to more accurately describe the interactions between the target and the ligands.Conclusion:The novel ensemble-QSAR model built in this study outperforms the traditional 3D-QSAR model in rationality, and provides a good example of selecting suitable protein structures for docking prediction and for building structure-based QSAR using available protein structures.
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Association between child and adolescent obesity and parental weight status: a cross-sectional study from rural North China.
J. Int. Med. Res.
PUBLISHED: 06-14-2013
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To investigate the relationship between child and adolescent obesity, and parental weight status.
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Fluoxetine attenuates chronic methamphetamine-induced pulmonary arterial remodelling: possible involvement of serotonin transporter and serotonin 1B receptor.
Basic Clin. Pharmacol. Toxicol.
PUBLISHED: 06-12-2013
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Epidemiological data have shown that methamphetamine (MA) abuse significantly increases the risk of developing pulmonary arterial hypertension (PAH). To investigate whether MA could induce PAH and its possible mechanism, rats were exposed daily to MA for 5 weeks in the absence or presence of fluoxetine. The results showed that the pulmonary arterial pressure was not significantly increased, but the pulmonary arterial remodelling was markedly developed in the MA exposure group. The protein expressions of the serotonin transporter (5-HTT) and 5-HT(1B) receptor were increased in the lungs and in the pulmonary arteries of MA-treated rats. Fluoxetine attenuated the pulmonary arterial remodelling and down-regulated the protein expression of 5-HTT and 5-HT(1B) receptor in pulmonary arteries of MA-treated rats. These findings suggest that fluoxetine has a novel potential suppressive effect on the chronic MA-induced pulmonary vascular remodelling and also suggest that 5-HTT and 5-HT(1B) receptor may be involved as part of its mechanism.
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Bioinformatics analysis of breast cancer bone metastasis related gene-CXCR4.
Asian Pac J Trop Med
PUBLISHED: 06-10-2013
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To analyze breast cancer bone metastasis related gene-CXCR4.
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Combined radiochemotherapy in patients with locally advanced pancreatic cancer: A meta-analysis.
World J. Gastroenterol.
PUBLISHED: 06-02-2013
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To compare the long-term clinical efficacy of chemotherapy plus radiotherapy (CRT) with that of radiotherapy alone (RT) or chemotherapy alone (CT) for locally advanced pancreatic carcinoma (LAPC).
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Degree-based attacks are not optimal for desynchronization in general networks.
Phys Rev E Stat Nonlin Soft Matter Phys
PUBLISHED: 05-23-2013
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Suppressing harmful synchronization in complex networked systems is receiving increasing interest in various contexts including epileptic seizure and the Internet and traffic congestion. It is traditionally believed that by disrupting the most important nodes (i.e., those nodes of high degrees) synchronization can be effectively mitigated. However, this approach can perform poorly in more general situations such as homogeneous (random) or small world networks. In this article, we investigate how topological properties, such as the heterogeneity of the network can have an impact on its desynchronization. In particular, we propose a topology-aware scheme that chooses topologically sufficiently diversified contrarian nodes by taking into account the patterns of connectivity among them. Hence, a maximal number of nodes in the network can be influenced, leading to a more global impact and greater disruption to synchronization. Our scheme demonstrates significantly improved performance on various networked systems including homogeneous networks, small world, and even scale-free networks.
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Association between obesity and ECG variables in children and adolescents: A cross-sectional study.
Exp Ther Med
PUBLISHED: 05-22-2013
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Obesity exhibits a wide variety of electrocardiogram (ECG) abnormalities in adults, which often lead to cardiovascular events. However, there is currently no evidence of an association between obesity and ECG variables in children and adolescents. The present study aimed to explore the associations between obesity and ECG intervals and axes in children and adolescents. A cross-sectional observational study of 5,556 students aged 5-18 years was performed. Anthropometric data, blood pressure and standard 12-lead ECGs were collected for each participant. ECG variables were measured manually based on the temporal alignment of simultaneous 12 leads using a CV200 ECG Work Station. Overweight and obese groups demonstrated significantly longer PR intervals, wider QRS durations and leftward shifts of frontal P-wave, QRS and T-wave axes, while the obese group also demonstrated significantly higher heart rates, compared with normal weight groups within normotensive or hypertensive subjects (P<0.05). Abdominal obesity was also associated with longer PR intervals, wider QRS duration and a leftward shift of frontal ECG axes compared with normal waist circumference (WC) within normotensive or hypertensive subjects (P<0.05). Gender was a possible factor affecting the ECG variables. Furthermore, the ECG variables, including PR interval, QRS duration and frontal P-wave, QRS and T-wave axes, were significantly linearly correlated with body mass index, WC and waist-to-height ratio adjusted for age, gender, ethnicity and blood pressure. However, there was no significant association between obesity and the corrected QT interval (P>0.05). The results of the current study indicate that in children and adolescents, general and abdominal obesity is associated with longer PR intervals, wider QRS duration and a leftward shift of frontal P-wave, QRS and T-wave axes, independent of age, gender, ethnicity and blood pressure.
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Biotransformation of osthole by Alternaria longipes.
J Asian Nat Prod Res
PUBLISHED: 05-16-2013
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The biotransformation of osthole (1) by Alternaria longipes was carried out, and five transformed products were obtained in the present research work. Based on their extensive spectral data, the structures of these metabolites were characterized as 4-hydroxyl-osthole (2), 4-hydroxyl-2,3-dihydroosthole (3), 2,3-dihydroxylosthole (4), osthole-4-oic acid methyl ester (5), and osthole-4-oic acid glucuron-1-yl ester (6), respectively. Among them, products 5 and 6 were new compounds.
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Registration of 3D point clouds and meshes: a survey from rigid to nonrigid.
IEEE Trans Vis Comput Graph
PUBLISHED: 05-11-2013
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Three-dimensional surface registration transforms multiple three-dimensional data sets into the same coordinate system so as to align overlapping components of these sets. Recent surveys have covered different aspects of either rigid or nonrigid registration, but seldom discuss them as a whole. Our study serves two purposes: 1) To give a comprehensive survey of both types of registration, focusing on three-dimensional point clouds and meshes and 2) to provide a better understanding of registration from the perspective of data fitting. Registration is closely related to data fitting in which it comprises three core interwoven components: model selection, correspondences and constraints, and optimization. Study of these components 1) provides a basis for comparison of the novelties of different techniques, 2) reveals the similarity of rigid and nonrigid registration in terms of problem representations, and 3) shows how overfitting arises in nonrigid registration and the reasons for increasing interest in intrinsic techniques. We further summarize some practical issues of registration which include initializations and evaluations, and discuss some of our own observations, insights and foreseeable research trends.
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[Effects of high glucose on in vitro invasiveness of human breast cancer cell line MDA-MB-435].
Zhonghua Yi Xue Za Zhi
PUBLISHED: 05-08-2013
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To explore the effects and underlying mechanisms of high glucose on in vitro invasiveness of human breast cancer cell line MDA-MB-435.
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[An individualized approach combining local flaps with radiotherapy for the treatment of auricle keloid].
Zhongguo Yi Xue Ke Xue Yuan Xue Bao
PUBLISHED: 05-07-2013
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To investigate an individualized approach to the treatment of auricle keloid to maintain the normal appearance of external ear and meanwhile reduce the recurrence.
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Association between ISL1 variants and susceptibility to ventricular septal defect in a Chinese cohort.
Mol Diagn Ther
PUBLISHED: 04-11-2013
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It has previously been reported that ISLET1 (ISL1) plays a fundamental role in cardiac morphogenesis. This study investigated the possible association between variants in the ISL LIM homeobox 1 (ISL1) gene and congenital ventricular septal defect (VSD) in a Chinese cohort.
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Efficacy of capecitabine and oxaliplatin regimen for extrahepatic metastasis of hepatocellular carcinoma following local treatments.
World J. Gastroenterol.
PUBLISHED: 03-21-2013
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To investigate the efficacy and safety of capecitabine and oxaliplatin (CapeOx) for extrahepatic metastasis after local treatment of hepatocellular carcinoma (HCC).
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Role of octreotide in post chemotherapy and/or radiotherapy diarrhea: prophylaxis or therapy?
Asia Pac J Clin Oncol
PUBLISHED: 01-08-2013
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AIM: The clinical efficacy of octreotide in controlling chemoradiotherapy-induced diarrhea remains controversial. We conducted a meta-analysis of randomized controlled trials (RCT) comparing octreotide with placebo in the prevention or therapy of chemoradiotherapy-induced diarrhea. METHODS: A computerized literature search of Medline, EMBASE, Cochrane Library and BIOSIS databases were performed for RCT comparing the use of octreotide versus placebo in the management of patients with chemoradiotherapy-induced diarrhea. The main outcome measure was the response of the diarrhea. RESULTS: We included eight RCT comprising 572 patients. We found octreotide was significantly effective compared with the placebo (OR, 4.9; 95%, 1.58-15.2).The overall effect of octreotide was 69% (204/294), while that of placebo was 54% (149/278). When divided into prophylactic and therapeutic subgroups, the prophylactic subgroup had an OR of 2.11 (95% CI, 0.51-2.89) for chemoradiotherapy-induced diarrhea and the therapeutic subgroup had an OR of 7.30 (95% CI, 4.09-13.04). CONCLUSION: In chemoradiotherapy-induced diarrhea, octreotide is best used as a therapeutic rather than a prophylactic agent against diarrhea.
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A SNP based high-density linkage map of Apis cerana reveals a high recombination rate similar to Apis mellifera.
PLoS ONE
PUBLISHED: 01-01-2013
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The Eastern honey bee, Apis cerana Fabricius, is distributed in southern and eastern Asia, from India and China to Korea and Japan and southeast to the Moluccas. This species is also widely kept for honey production besides Apis mellifera. Apis cerana is also a model organism for studying social behavior, caste determination, mating biology, sexual selection, and host-parasite interactions. Few resources are available for molecular research in this species, and a linkage map was never constructed. A linkage map is a prerequisite for quantitative trait loci mapping and for analyzing genome structure. We used the Chinese honey bee, Apis cerana cerana to construct the first linkage map in the Eastern honey bee.
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[Effect of comprehensive control in demonstration plots of parasitic diseases in Guizhou Province].
Zhongguo Xue Xi Chong Bing Fang Zhi Za Zhi
PUBLISHED: 11-30-2011
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To evaluate the effect of comprehensive control in demonstration plots of parasitic diseases in Guizhou Province.
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Association of tag SNPs of GPx-3 with essential hypertension in rural Han Chinese in Fuxin, Liaoning, China.
Chin. Med. J.
PUBLISHED: 09-22-2011
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Genetic mechanisms contribute to blood pressure regulation. This study investigated whether glutathione peroxidase (GPx-3) tag single nucleotide polymorphisms (SNPs) are associated with hypertension in the rural areas of Fuxin county, Liaoning province, China.
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[Distribution of HIV-1 subtype and its relationship with HIV/AIDS epidemic in Guizhou province].
Zhonghua Liu Xing Bing Xue Za Zhi
PUBLISHED: 09-22-2011
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To study the HIV-1 diversity and how did it promote the rapid spread of AIDS, in Guizhou province.
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[Deviation of ST-segment and detection of infarction related artery in patients with acute inferior wall infarction].
Zhonghua Yi Xue Za Zhi
PUBLISHED: 09-15-2011
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To explore the characteristics of ST-segment deviation in patients with acute ST elevation myocardial infarction(STEMI)having only one vessel lesion in either left circumflex artery (LCX) or right coronary artery (RCA).
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N-palmitoylethanolamide, an endocannabinoid, exhibits antidepressant effects in the forced swim test and the tail suspension test in mice.
Pharmacol Rep
PUBLISHED: 08-23-2011
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The antidepressant-like effects of N-palmitoylethanolamide (PEA), a putative endocannabinoid, was investigated in mice using the tail suspension test (TST) and the forced swimming test (FST). In TST, PEA (10, 20, and 40 mg/kg) produced a statistically significant reduction in immobility (50, 32, and 34%, respectively, vs. the control group), whereas fluoxetine (20 mg/kg) reduced immobility by 38%. In FST, PEA (5, 10, and 20 mg/kg) produced a statistically significant reduction in immobility (15, 21, and 36%, respectively), whereas fluoxetine (20 mg/kg) reduced immobility by 18%. Moreover, PEA (20 mg/kg) did not significantly change motor activity in a spontaneous behavioral test. In conclusion, PEA (dose range of 5-40 mg/kg) administered orally reduced immobility in TST and FST, comparable to the antidepressant effect of fluoxetine, and had no effect on spontaneous activity in mice.
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[Electrochemical sensor for acetaminophen based on layer-by-layer self assembly technique].
Yao Xue Xue Bao
PUBLISHED: 06-28-2011
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A novel type of carbon nanotube-coated Au nanoparticle and [bmim]BF4 composite modified glassy carbon electrode was fabricated by a layer-by-layer self-assembly technique. The electrochemical performance of acetaminophen (ACOP) on the modified electrode was investigated by cyclic voltammetry. The Nafion/GNPs/RTIL/MWNTs/GC electrode showed an excellent electrocatalytic activity for the oxidation of ACOP and accelerated electron transfer between the electrode and ACOP. For ACOP, the reversible electrochemical process was observed on the Nafion/GNPs/RTIL/MWNTs/GC electrode, while irreversible electrochemical process occurred on the GC electrode. For the Nafion/GNPs/RTIL/MWNTs/GC electrode, the anodic peak potential of ACOP was moved from 0.562 V to 0.413 V, with a potential drop of 149 mV. At the same time, the reduction peak potential was 0.384 V, and the potential difference was only 29 mV. It was shown that the modified electrode possessed higher electrocatalytic activity and more sensitive effect for the detection of ACOP than both MWNTs/GC electrode and GC electrode. The effects of the different experimental conditions on the electrochemical behaviors of ACOP were explored. Under the optimum conditions of preparation and experimental, the linear calibration curves of ACOP were obtained in a wide range of 2 x 10(-1) to 4.0 x 10(-4) mol x L(-1) with a correlation coefficient 0.999 2 and a detection limit of 2.6 x 10(-8) mol x L(-1) (the ratio of signal to noise, 3:1). The recovery rate was 97.9%-100.8%. This method can be used to determine ACOP in paracetamol tablets with satisfactory results.
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[ST segment deviation in acute myocardial infarction with isolated disease of left anterior descending artery].
Zhonghua Yi Xue Za Zhi
PUBLISHED: 05-26-2011
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To analyze the characteristics of the electrocardiogram (ECG) of left anterior descending coronary artery (LAD) occlusion in patients with acute ST elevation myocardial infarction (STEMI).
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Transgenic mice with overexpression of mutated human optineurin(E50K) in the retina.
Mol. Biol. Rep.
PUBLISHED: 05-05-2011
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In the present work, Site-directed mutagenesis to insert the Glu50Lys amino acid substitution was achieved by PCR using plasmid pBluescript-OPTN. Mutated human OPTN(E50K) gene-driven mouse c-kit promoter was constructed and confirmed by endonuclease digestion and sequence analysis. Transgenic mice were generated via the microinjection method. PCR and DNA dot blot were used to screen the positive transgenic mice. RT-PCR analyzed the RNA level and location of mutated human OPTN(E50K) mRNA expression in transgenic mice. Western blot and immunohistochemistry were used to detect the level and location of mutated human OPTN(E50K) expression in transgenic mice. A transgenic mouse model with overexpression of mutated human OPTN(E50K) in retina was successfully established. The transgene was integrated and transmitted into the chromosome of transgenic mice. Mutated human OPTN(E50K) gene was controlled by c-kit promoter and expressed in the retina in mice. Mutated human OPTN(E50K) in transgenic mice was higher than that of wild type C57BL/6J mice. Our studies had provided a new transgenic model for investigating the molecular properties of mutated human OPTN(E50K).
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[Design and realization of a microarray data analysis platform].
Nan Fang Yi Ke Da Xue Xue Bao
PUBLISHED: 04-26-2011
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To design a platform for microarray data analysis and processing in the browser/server mode running in Linux operating system.
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Identification of a novel FBN1 gene mutation in a Chinese family with Marfan syndrome.
Mol. Vis.
PUBLISHED: 04-22-2011
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To identify the mutation in the fibrillin-1 gene (FBN1) in a Chinese family with Marfan syndrome (MFS).
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Enrichment of a common wheat genetic map and QTL mapping for fatty acid content in grain.
Plant Sci.
PUBLISHED: 01-26-2011
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DArT and SSR markers were used to saturate and improve a previous genetic map of RILs derived from the cross Chuan35050 × Shannong483. The new map comprised 719 loci, 561 of which were located on specific chromosomes, giving a total map length of 4008.4 cM; the rest 158 loci were mapped to the most likely intervals. The average chromosome length was 190.9 cM and the marker density was 7.15 cM per marker interval. Among the 719 loci, the majority of marker loci were DArTs (361); the rest included 170 SSRs, 100 EST-SSRs, and 88 other molecular and biochemical loci. QTL mapping for fatty acid content in wheat grain was conducted in this study. Forty QTLs were detected in different environments, with single QTL explaining 3.6-58.1% of the phenotypic variations. These QTLs were distributed on 16 chromosomes. Twenty-two QTLs showed positive additive effects, with Chuan35050 increasing the QTL effects, whereas 18 QTLs were negative with increasing effects from Shannong483. Six sets of co-located QTLs for different traits occurred on chromosomes 1B, 1D, 2D, 5D, and 6B.
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[Analysis on the correlation between SF-36 and QOL-35 in patients and coronary artery disease.]
Zhonghua Liu Xing Bing Xue Za Zhi
PUBLISHED: 12-24-2010
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OBJECTIVE: To analyze the correlation between SF-36 and QOL-35 and the consistency of the two kinds of life-quality questionnaires when evaluating the quality of life in patients with coronary heart disease. METHODS: 781 in-hospital-patients, who underwent coronary angiography from June 2008 to April 2009, were included in this study. Quality of life was measured by the Chinese versions of SF-36 and QOL-35. Demographic and clinical information were collected. Correlation Analysis was done between SF-36 and QOL-35 in the patients with coronary artery disease. RESULTS: The total score of SF-36 was 62.63 ± 12.47 and the QOL-35 total score was 62.70 ± 9.69. Data from the simple correlation analysis showed that SF-36 and QOL-35 total scores (r = 0.725, P < 0.01), SF-36 physical functioning and QOL-35 independent living capacity (r = 0.933, P < 0.01), SF-36 mental health and QOL-35 mental health(r = 0.132, P < 0.01), SF-36 social function and QOL-35 social function score (r = 0.215, P < 0.01) were all relevant. Canonical correlation analysis showed that there was significant correlation between SF-36 and QOL-35 (r = 0.946, P < 0.01). CONCLUSION: SF-36 and QOL-35 were consistent in the evaluation on the quality of life, at the overall level.
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[Surface-enhanced Raman scattering spectra of Rh6G adsorbed on Ag nanowire arrays].
Guang Pu Xue Yu Guang Pu Fen Xi
PUBLISHED: 11-26-2010
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The degree of order and uniform of substrates is the key to surface-enhanced Raman scattering study. The structure of porous alumina template by anodic oxidation method is ordered and uniform and the template could be used as the substrate of SERS. Nanowire arrays of Ag were fabricated from the template of porous alumina. The authors observed the SERS signals of the Rh6G adsorbed on the Ag nanowire arrays. The results revealed that the SERS intensity depended on the length of the wires that emerged on the surface that was controlled by the etching time, but the frequency was not affected by the surface condition; the results also revealed that PO4(3-) can increase the intensity of SERS signals.
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Low-temperature heat capacities of 1-alkyl-3-methylimidazolium bis(oxalato)borate ionic liquids and the influence of anion structural characteristics on thermodynamic properties.
Phys Chem Chem Phys
PUBLISHED: 11-23-2010
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Two chelated orthoborate ionic liquids (ILs), 1-butyl-3-methylimidazolium bis(oxalato)borate ([Bmim][BOB]) and 1-hexyl-3-methylimidazolium bis(oxalato)borate ([Hmim][BOB]), were prepared and characterized. Their thermodynamic properties were studied using adiabatic calorimetry and differential scanning calorimetry (DSC). The thermodynamic properties of the two ILs were evaluated and compared with each other, and then with those of other [Bmim] type ILs. The results clearly indicate that for a given cation (or anion) and at a certain temperature, the more atoms in the anion (or cation), the higher the heat capacity; the higher glass-transition temperatures of [BOB] type ILs than others are mainly caused by the higher symmetry of the orthoborate anion structure. It is suggested that a high content of strong electronegative atoms and C(n) or C(nv) (n = 1,2,3,…,?) point group symmetry in the anion are favorable for the design and synthesis of room temperature ILs with a wide liquid range.
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[Influence of arbuscular mycorrhizal inoculation on growth and phoxim residue of carrot (Daucus carota L.)].
Huan Jing Ke Xue
PUBLISHED: 11-10-2010
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A pot culture experiment was carried out to study the influence of arbuscular mycorrhizal (AM) fungi on the growth and phoxim residue of carrot (Daucus carota L). Four levels of phoxim (0, 200, 400, 800 mg x L(-1)) and two AM fungal inocula, Glomus intraradices BEG 141(141), Glomus mosseae BEG 167 (167),and one nonmycorrhizal inoculum (CK), were applied to the sterilized soil. The plants were harvested after 5 months of growth and phoxim was irrigated into the root zone 14 d before plant harvest. Although decreasing with the increase of phoxim dosage, root infection rates of all the mycorrhizal plants were higher than 70%. Phoxim showed no significant dose effect on shoot wet weights and root yields, which were all increased by AM inoculation at four phoxim dosages. Phoxim residues in shoots and roots increased with the increase of phoxim dosage, but decreased by AM inoculation. In general, Glomus intraradices BEG 141 showed more pronounced effects on the growth and phoxim residue of carrot than Glomus mosseae BEG 167 did. Our results show a promising potential of AM fungi in carrot production and controlling pesticide residues.
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[Prevalence, awareness, treatment and control of hypertension in rural adult residents of Liaoning Province].
Zhonghua Xin Xue Guan Bing Za Zhi
PUBLISHED: 11-09-2010
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The objective of this study was to estimate the current status of prevalence, awareness, treatment and control rate of hypertension in adult residents living in rural area of Liaoning Province, North China.
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Synthesis and primary anticonvulsant activity evaluation of 6-alkyoxyl-tetrazolo [5,1-a]phthalazine derivatives.
Arzneimittelforschung
PUBLISHED: 07-23-2010
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A new series of 6-alkyoxyl-tetrazolo[5,1-a] phthalazine derivatives (4 a-4 o) were synthesized as potential anticonvulsant agents. Anticonvulsant activity was evaluated by the maximal electroshock (MES) test. Neurotoxicity was evaluated by the rotarod neurotoxicity test. The pharmacological results showed that 6-(4-chlorophenyoxyl)-tetrazolo[5,1-a]phthalazine (4 m) was the most potent agent, with a median effective dose (ED50) of 6.8 mg/kg and a median neurotoxic dose (TD50) of 456.4 mg/kg. The protective index (PI = TD50/ED50) for compound 4 m was 67.1, which was significantly higher than that for the reference drug carbamazepine (PI = 6.4).
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[Characteristics of chronic obstructive pulmonary disease phenotypes based on high-resolution CT and the relationship with interleukin-6].
Zhonghua Jie He He Hu Xi Za Zhi
PUBLISHED: 07-22-2010
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To classify the high-resolution CT (HRCT) phenotypes of COPD, and to investigate the clinical characteristics of various phenotypes and the relationship with airway inflammation.
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Induced synchronization of a mobile agent network by phase locking.
Phys Rev E Stat Nonlin Soft Matter Phys
PUBLISHED: 06-17-2010
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We investigate synchronization issues of a set of mobile agents in plane, where each agent carries an identical chaotic oscillator and interacts with its neighbors through a blinking coupling mechanism. We discuss the effect of blinking pattern on synchronization of the related network. In particular, we show that phase locking of the blinking behavior can dramatically enhance synchronizability of the mobile agent network, while the random blinking pattern works little. Also, we show that by assessing the convergence time, the mobile agent networks with different blinking periods and duty ratios share a common idle duration which is independent of both the blinking period and the corresponding duty ratio.
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Evaluation of the anticonvulsant activity of 6-(4-chlorophenyoxy)-tetrazolo[5,1-a]phthalazine in various experimental seizure models in mice.
Pharmacol Rep
PUBLISHED: 05-29-2010
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This study investigated the anticonvulsant activity of a new phthalazine tetrazole derivative, QUAN-0808 (6-(4-chlorophenoxy)-tetrazolo[5,1-a]phthalazine), in the mouse maximal electroshock (MES) seizure model. The neurotoxicity of QUAN-0808 was investigated using the rotarod neurotoxicity test in mice. QUAN-0808 exhibited higher activity (median effective dose, ED(50) = 6.8 mg/kg) and lower neurotoxicity (median toxic dose, TD(50) = 456.4 mg/kg), resulting in a higher protective index (PI = 67.1) compared with carbamazepine (PI = 6.4). In addition, QUAN-0808 exhibited significant oral anticonvulsant activity (ED(50) = 24 mg/kg) against MES-induced seizure with low neurotoxicity (TD(50) > 4500 mg/kg) in mice, resulting in a PI value of more than 187.5. QUAN-0808 was also tested in chemically induced animal models of seizure (pentylenetetrazole [PTZ], isoniazid [ISO], thiosemicarbazide [THIO] and 3-mercaptopropionic acid [3-MP]) to further investigate the anticonvulsant activity; QUAN-0808 produced significant anticonvulsant activity against seizures induced by ISO, THIO and 3-MP.
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Synthesis and anti-inflammatory activity evaluation of some novel 6-alkoxy(phenoxy)-[1,2,4]triazolo[3,4-a]phthalazine-3-amine derivatives.
Eur J Med Chem
PUBLISHED: 05-21-2010
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Starting from phthalic anhydride, several new 6-alkoxy(phenoxy)-[1,2,4]triazolo[3,4-a]phthalazine-3-amine derivatives were synthesized as potent anti-inflammatory agent. The study showed that the compounds 6h (6-(2-chlorophenoxy)-[1,2,4]triazolo[3,4-a]phthalazine-3-amine) and 6s (6-(4-aminophenoxy)-[1,2,4] triazolo[3,4-a]phthalazine-3-amine) exhibited the highest anti-inflammatory activity (81% and 83% inhibition, respectively, at 0.5 h after i.p. administration) which were slightly more potent than the reference drug Ibuprofen (61%). Furthermore, the peak activity of 6h and 6s was observed at the 3 h after p.o. administration, and they exhibited stronger anti-inflammatory activity than Ibuprofen at the dose of 50 mg/kg at the peak time.
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[Analysis of therapeutic effects of rural patients with hypertension by combination administration of low dosage of hydrochlorothiazide and nitrendipine].
Zhonghua Xin Xue Guan Bing Za Zhi
PUBLISHED: 04-20-2010
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To evaluate the therapeutic effects of combination administration of hydrochlorothiazide and nitrendipine at low dosage in the treatment of rural hypertension patients.
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[Effects of low temperature- and weak light stress and its recovery on the photosynthesis and chlorophyll fluorescence parameters of cut flower chrysanthemum].
Ying Yong Sheng Tai Xue Bao
PUBLISHED: 04-15-2010
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The cut flower chrysanthemum Jinba was respectively treated with lower temperature and weaker light (16 degrees C/ 12 degrees C, PFD 100 micromol x m(-2) x s(-1)) and critical low temperature and weak light (12 degrees C/8 degrees C, PFD 60 micromol x m(-2) x s(-1)) for 11 days, and then transferred to normal condition (22 degrees C/18 degrees C, PFD 450 micromol x m(-2) x s(-1)) for 11 days, aimed to study the low temperature- and weak light stress and its recovery on the photosynthesis and chlorophyll fluorescence of chrysanthemum leaves. Under the stress of lower temperature and weaker light, the net photosynthetic rate (P(n)) and stomatal limitation (L(s)) of chrysanthemum leaves decreased while the intercellular CO2 concentration (C(i)) increased, the maximal photochemical efficiency of PS II (F(v)/F(m)) in dark and the initial fluorescence (F(o)) had no obvious change, but the maximal photochemical efficiency of PS II (F(v)/F(m)) in light increased after an initial decrease. Contrarily, under the stress of critical low temperature and weak light, the F(o) increased, and the F(v)/F(m) and F(v)/F(m) decreased significantly. The quantum yield of PS II electron transport (phi(PS II)), photochemical quenching (q(p)), and apparent photosynthetic electron transfer rate (ETR) of chrysanthemum leaves decreased with increasing stress and time, and recovered quickly after the release of lower temperature- and weaker light stress but more slowly after the release of critical low temperature- and weak light stress. At the same time, the photochemistry react rate (Prate) decreased, but the hot dissipation of antenna (Drate) and the energy dissipation of PS II (Ex) increased under the stress conditions. Drate was the main pathway of superfluous light allocation.
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[Covariance analysis on the change of quality of life affected by percutaneous coronary intervention for patients with coronary heart disease].
Zhonghua Liu Xing Bing Xue Za Zhi
PUBLISHED: 03-23-2010
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To compare changes of quality of life at 3 months from baseline among patients who underwent and did not undergo percutaneous coronary intervention (PCI).
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STK39 is an independent risk factor for male hypertension in Han Chinese.
Int. J. Cardiol.
PUBLISHED: 03-20-2010
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STK39 interacts with OXSR1 and phosphorylates the sodium-chloride co-transporter (SLC12A3), which plays a critical role in regulating the salt/water balance and blood pressure. Here we tested whether STK39, OXSR1, and SLC12A3 genetically contribute to hypertension in the Han Chinese population and how the SNP to SNP or SNP to other risk factors interacts in the pathogenesis of hypertension.
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Ceramide from sphingomyelin hydrolysis differentially mediates mitogen-activated protein kinases (MAPKs) activation following cerebral ischemia in rat hippocampal CA1 subregion.
J Biomed Res
PUBLISHED: 03-01-2010
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To explore the role that ceramide plays in the activation of mitogen-activated protein kinases (MAPKs) during cerebral ischemia and reperfusion.
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[Protective effects of hypoxia-inducible factor-1 alpha on myocardial ischemia/reperfusion injury in rat and the role of protein kinase C in signal pathway].
Zhongguo Wei Zhong Bing Ji Jiu Yi Xue
PUBLISHED: 02-23-2010
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To study the expression of hypoxia-inducible factor-1 alpha (HIF-1 alpha) in a rat model of myocardial ischemia/reperfusion injury (IRI) and the role of protein kinase C (PKC) in signal pathway.
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[Design and construction of the platform for comparative genomics].
Nan Fang Yi Ke Da Xue Xue Bao
PUBLISHED: 02-18-2010
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To design a versatile genome comparison and visualization platform based on browser/server mode supported by a local server.
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JoVE Visualize is a tool created to match the last 5 years of PubMed publications to methods in JoVE's video library.

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In developing our video relationships, we compare around 5 million PubMed articles to our library of over 4,500 methods videos. In some cases the language used in the PubMed abstracts makes matching that content to a JoVE video difficult. In other cases, there happens not to be any content in our video library that is relevant to the topic of a given abstract. In these cases, our algorithms are trying their best to display videos with relevant content, which can sometimes result in matched videos with only a slight relation.