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Find video protocols related to scientific articles indexed in Pubmed.
The Macrophage Migration Inhibitory Factor -173G/C gene polymorphism increase the risk of renal disease: A meta-analysis.
Nephrology (Carlton)
PUBLISHED: 10-12-2014
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Macrophage migration inhibitory factor (MIF) -173G/C (rs755622) gene polymorphism has been implicated the association with renal disease risk. However, lots of studies have reported inconclusive results. Therefore we performed a meta-analysis to investigate the relationship between the MIF -173G/C gene polymorphism and renal disease susceptibility.
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Associations between the rs6010620 polymorphism in RTEL1 and risk of glioma: a meta-analysis of 20,711 Participants.
Asian Pac. J. Cancer Prev.
PUBLISHED: 09-18-2014
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Associations between the rs6010620 polymorphism in the regulator of telomere elongation helicase1 (RTEL1) gene and glioma have been widely reported but the results were not inconclusive. The aim of the current study was to investigate the association between the rs6010620 polymorphism in RTEL1 gene and risk of glioma by meta-analysis.
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Cu?O template synthesis of high-performance PtCu alloy yolk-shell cube catalysts for direct methanol fuel cells.
Chem. Commun. (Camb.)
PUBLISHED: 09-04-2014
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Novel PtCu alloy yolk-shell cubes were fabricated via the disproportionation and displacement reactions in Cu2O yolk-shell cubes, and they exhibit significantly improved catalytic activity and durability for methanol electrooxidation.
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Macrolide antibiotics for treatment of asthma in adults: A meta-analysis of 18 randomized controlled clinical studies.
Pulm Pharmacol Ther
PUBLISHED: 07-28-2014
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Mounting studies have been showed that long-term macrolides used in patients with asthma could improve the lung function and symptoms. However, a large number of studies have reported inconclusive results. The aim of this meta-analysis was to investigate the effect of macrolide antibiotics in patients with asthma. We have performed a search in PubMed, Embase, China National Knowledge Internet (CNKI), and Wanfang databases. The weighed mean difference (WMD) or standardized mean difference (SMD) was used to evaluate the pooled effect. Statistical analysis was performed by STATA 11.0 software. Totally 1306 patients were included in the meta-analysis. The overall results indicated that statistically significance of long-term macrolides therapy in patients with asthma on forced expiratory volume in 1 s (FEV1) (WMD: 0.11, P < 0.01), peak expiratory flow (PEF) (SMD: 0.25, P = 0.001), airway hyper-responsiveness (AHR) (SMD: 0.90, P = 0.04), forced vital capacity (FVC) (WMD: 0.18, P = 0.05) and FEV1/FVC (WMD: 1.93, P < 0.001), but no statistically significance on FEV1/predict, FVC/predict, symptom scores, quality of life scores (QOL), reliever inhaler puffs per 24 h, and cell counts in sputum and blood. The subgroup analysis indicated macrolides could increase FEV1 and PEF in Caucasian and Asian, decrease AHR in Caucasian, while cells counts of sputum improvement among Asian. Therefore, the study suggested that long-term marolides therapy in asthma may improved the FEV1, PEF, AHR, FVC, FEV1/FVC and cells counts in sputum, but it can't improve other lung function (FEV1/predict and FVC/predict) and clinical outcomes (such as symptom, quality of life etc.).
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[Comparative analysis of variable region of white spot syndrome virus genome in Penaeus vannamei in Guangxi, China].
Bing Du Xue Bao
PUBLISHED: 04-30-2014
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Comparative analysis of variable region ORF14/15 genes of white spot syndrome virus (WSSV) genome in Guangxi Penaeus vannamei (P. vannamei) could provide useful information for the evaluation of genetic diversity and genetic evolutionary relationship among WSSV isolates from Guangxi, China and other places. Based on geographical and temporal considerations, 40 WSSV-positive P. vannamei samples were collected during the period between May 2010 and July 2013 from Beihai, Qinzhou, and Fangchenggang, which were the main P. vannamei production areas in Guangxi, and the variable region ORF14/15 genes of the WSSV genome from all infected samples were amplified by PCR and then subjected to cloning and sequence analysis. Pairwise and multiple alignment analysis was then conducted to evaluate the degree of genetic divergence between different strains. The variable region ORF14/15 genes from 25 of 40 WSSV positive samples were successfully cloned and sequenced; among the ORF14/15 genes of 25 WSSV-positive strains, 22 was 619 bp in length and 3 was 620 bp. All the 25 Guangxi strains carried a 5949-bp deletion in the ORF14/15 region relative to TH-96-II, which has the longest nucleotide sequence in this region; the deletion of Guangxi strains occurred in the middle region of ORF14/15 gene, with only 190 bp and 429 bp/ 430 bp at 5' and 3' ends, respectively, which were coincident with WSSV-IN-05-I in deletion length and position. Sixteen of 25 Guangxi strains had completely identical nucleotide sequences in the variable re gion, and the homology between other strains also exceeded 97.9%. There were single nucleotide substi tution, deletion, and insertion in the ORF14/15 region of Guangxi strains compared with other strains in GenBank. In the phylogenetic tree based on WSSV variable region ORF14/15, the Guangxi strains were closely related and formed a separate branch with Indian strain IN-05-I, but far from other strains in GenBank. The ORF14/15 gene of WSSV isolates in cultured P. vannamei in Guangxi has a large deletion in the middle of the variable region, and the Guangxi WSSV strains show no significant spatio-temporal differences; the Guangxi strains are closer in genetics to Indian strain IN-05-I than other strains in GenBank.
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The association between CD14-260C/T polymorphism and malignant tumor risk: a meta-analysis of 5,603 participants.
Tumour Biol.
PUBLISHED: 04-29-2014
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The CD14-260C/T polymorphism has been implicated to be in association with malignant tumor. However, a number of studies have reported inconclusive results. The aim of this study was to investigate the relationship of CD14-260C/T polymorphism and malignant tumor risk by meta-analysis. A search was performed in PubMed, Embase, the Chinese Journals Full-text Database (CNKI), and Wanfang databases up to August 2013. Odds ratio (OR) and 95 % confidence interval (95 % CI) were used to assess the association. Statistical analysis was calculated by STATA 11.0 software. The polymorphism was identified from 11 articles (12 case-control studies), involving 2,660 cases and 2,943 controls. Overall, no significant association between CD14-260C/T polymorphism and malignant tumor risk was found in the dominant model (TT + TC vs. CC: OR?=?0.86, 95 % CI?=?0.67-1.11). In the subgroup analysis by malignant tumor types, we found that the heterozygote model (TC vs. CC) might reduce the risk of malignant tumor, especially hematological malignance and prostate cancer (OR?=?0.67, 95 % CI?=?0.47-0.95), but not associated with gastrointestinal cancer susceptibility. In the subgroup analysis by ethnicity, no significant associations were found among different ethnicities. The study suggested that CD14-260C/T polymorphism might be a protective factor for hematological malignance and prostate tumor susceptibility but not an independent risk factor for gastrointestinal cancer susceptibility. To further evaluate the association between the polymorphism and malignant tumor susceptibility, more studies involving thousands of patients are required.
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Mitochondrial DNA sequence of Culter compressocorpus.
Mitochondrial DNA
PUBLISHED: 04-18-2014
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Abstract In this study, the complete mitochondrial genome of Culter compressocorpus was detected and annotated. The circular mtDNA molecule was 16,623?bp in length which contains 22 transfer RNA genes, 13 protein-coding genes, 2 ribosomal RNA genes, and the non-coding control region (D-loop). Its total protein-coding genes content is 68.67% in whole mitochondrial genome. The mitochondrial genome can contribute to the studies on geographical distribution and genetic diversity of C. compressocorpus resources, as well as molecular phylogeny and species identification in Cyprinidae.
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Genome-wide analysis of DNA 5-hmC in peripheral blood of uremia by hMeDIP-chip.
Ren Fail
PUBLISHED: 04-03-2014
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Treatment of uremia is now dominated by dialysis; in some cases, patients are treated with dialysis for decades, but overall outcomes are disappointing. A number of studies have confirmed the relevance of several experimental insights to the pathogenesis of uremia, but the specific biomarkers of uremia have not been fully elucidated. To date, our knowledge about the alterations in DNA 5-hydroxymethylcytosine (5-hmC) in uremia is unclear, to investigate the role of DNA 5-hmC in the onset of uremia, we performed hMeDIP-chip between the uremia patients and the normal controls from the experiment to identify differentially expressed 5-hmC in uremia-associated samples.
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Mitochondrial DNA sequence of Pseudorasbora parva (Cyprinidae: Gobioninae).
Mitochondrial DNA
PUBLISHED: 03-14-2014
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Abstract In this study, the complete mitochondrial genome of Pseudorasbora parva (Cyprinidae: Gobioninae) was determined. The circular mtDNA molecule was 16,601?bp in length which contains 22 transfer RNA genes, 13 protein-coding genes, 2 ribosomal RNA genes and non-coding control region (D-loop). The critical central conserved sequences were also detected. Its total A + T content is 55.97%. The mitochondrial genome can contribute to the studies on geographical distribution and genetic diversity of P. parva resources, as well as molecular phylogeny and species identification in Cyprinidae.
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COMT 158G/A and CYP1B1 432C/G polymorphisms increase the risk of endometriosis and adenomyosis: a meta-analysis.
Eur. J. Obstet. Gynecol. Reprod. Biol.
PUBLISHED: 03-10-2014
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Catechol-O-methyltransferase (COMT) 158G/A and cytochrome P450-1B1 (CYP1B1) 432C/G gene polymorphisms have been associated with the risk of endometriosis and adenomyosis, but results remain inconclusive. The aim of this study was to investigate the relationships between these polymorphisms and the risk of endometriosis and adenomyosis by meta-analysis.
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Carbon/MnO(2) double-walled nanotube arrays with fast ion and electron transmission for high-performance supercapacitors.
ACS Appl Mater Interfaces
PUBLISHED: 02-17-2014
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The novel carbon (C)/MnO2 double-walled nanotube arrays (DNTAs) are designed and fabricated via template-assisted electrodeposition. The unique DNTA architectures of C/MnO2 composites with high weight fraction of MnO2 allow high electrode utilization ratio and facilitate electron and ion transmission. In the half-cell test, the hybrid C/MnO2 DNTAs as electrodes show a large specific capacitance (Csp) of 793 F/g at the scan rate of 5 mV/s, high energy/power densities, and much enhanced long-term cycle stability. After 5,000 cycles, the Csp retention of C/MnO2 DNTAs keeps ?97%, which is much larger than 69% of the MnO2 nanotube arrays (NTAs). The symmetrical supercapacitors (SSCs) composed of C/MnO2 DNTAs also show the predominant performance, such as large Csp of 161 F/g and high energy density of ?35 Wh/kg, indicating that the C/MnO2 DNTAs is a potential electrode for supercapacitors. The high order pore passages, double-walled structures, hollow structures, and high conductivity are responsible for the superior performance of C/MnO2 DNTAs. Such hybrid C/MnO2 DNTAs may bring new opportunities for the development of supercapacitors with superior performance.
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The complete mitochondrial genome sequence of Hemiculter leucisculus.
Mitochondrial DNA
PUBLISHED: 01-28-2014
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Abstract The complete mitochondrial genome of Hemiculter leucisculus was determined to be 16,617?bp. It contains the structure of 22 transfer RNA genes, 13 protein-coding genes, 2 ribosomal RNA genes, and non-coding control region (D-loop). The critical central conserved sequences (CSB-D, CSB-E, and CSB-F) were also detected. The determination of H. leucisculus mitogenome would play an important role in genetic diversity and population vitality in Cyprinidae.
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Complete mitochondrial genome sequence of Romanogobio tenuicorpus (Amur whitefin gudgeon).
Mitochondrial DNA
PUBLISHED: 01-14-2014
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Abstract Amur whitefin gudgeon (Romanogobio tenuicorpus) belongs to the family Cyprinidae, it is freshwater aquaculture species in China. In the report, we determined the complete mitochondrial genome sequence of Romanogobio tenuicorpus, which is 16,600?bp long circular molecule with 13 protein-coding genes, 22 tRNA genes, 2 rRNA genes and a control region, the conserved sequence blocks, CSB1, CSB2 and CSB3 were also detected.
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Study on the association between the Arg194Trp polymorphism in the XRCC1 gene and the risk of hematological malignancies.
Tumour Biol.
PUBLISHED: 01-12-2014
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The association between the Arg194Trp polymorphism in the XRCC1 gene and the risk of hematological malignancies has been extensively investigated. However, the results were inconsistent. The objective of the current study is to investigate the association by meta-analysis. We searched the PubMed, Embase, CNKI, Wanfang, and Weipu databases, covering all studies until Aug. 7, 2013. Statistical analysis was performed by using the RevMan4.2 software and the Stata10.0 software. A total of 20 case-control studies concerning the Arg194Trp polymorphism were indentified from 19 articles. In total analysis, our results suggested that the Arg194Trp polymorphism was not associated with an increased/decreased risk of hematological malignancies (odds ratio (OR)?= 1.01, 95 % confidence interval (CI)?= 0.85-1.22, P = 0.87 for Arg/Trp+Trp/Trp vs. Arg/Arg). In the subgroup analysis by ethnicity, no significant association was found either among Asians (OR = 1.04, 95% CI = 0.84-1.29, P = 0.72) or among Europeans (OR = 1.04, 95% CI = 0.72-1.49, P = 0.83); in the subgroup analyses by cancer types, no significant association was found either among leukemia (OR = 1.10, 95% CI = 0.89-1.35, P = 0.39) or in lymphoma (OR = 0.83, 95% CI = 0.57-1.22, P = 0.35). The current meta-analysis indicated that the Arg194Trp polymorphism in the XRCC1 gene might be not a risk factor for hematological malignancies. In the future, more large-scale case-control studies are needed to validate these results.
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Minimally invasive midvastus versus standard parapatellar approach in total knee arthroplasty: a meta-analysis of randomized controlled trials.
PLoS ONE
PUBLISHED: 01-01-2014
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Minimally invasive midvastus approach (mini-midvastus) has been widely used in total knee arthroplasty (TKA). However, the clinical effects still remains controversial. This meta-analysis was based on randomized controlled trials (RCTs) aiming to quantitatively analyze the clinical efficacy of mini-midvastus versus standard parapatellar approach in TKA.
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Metformin inhibits the IL-6-induced epithelial-mesenchymal transition and lung adenocarcinoma growth and metastasis.
PLoS ONE
PUBLISHED: 01-01-2014
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Epithelial-mesenchymal transition (EMT) plays an important role in cancer tumorigenesis. However, the underlying mechanisms of EMT in lung adenocarcinoma, and how this process might be inhibited, remain to be explored. This study investigated the role of IL-6 in lung adenocarcinoma cell EMT and explored the potential effects of metformin on this process.
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The complete mitochondrial genome sequence of white-spotted char (Salvelinus leucomaenis).
Mitochondrial DNA
PUBLISHED: 12-12-2013
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Abstract The complete sequence of the mitochondrial genome of the white-spotted char (Salvelinus leucomaenis) was determined to be 16,658?bp in length, which contains the control region (CR), the origin of light-strand replication (OL), 22 transfer RNA genes, 2 ribosomal genes and 13 protein-coding genes. Overall, base composition of the complete mitochondrial DNA was 28.20% A, 26.51% T, 28.39% C, 16.90% G, with 54.71% AT.
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Design of Polypyrrole/Polyaniline Double-Walled Nanotube Arrays for Electrochemical Energy Storage.
ACS Appl Mater Interfaces
PUBLISHED: 12-11-2013
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The novel hybrid polypyrrole (PPy)/polyaniline (PANI) double-walled nanotube arrays (DNTAs) were designed to exploit the synergistic effects and shape effects for supercapacitive energy storage. The PPy/PANI DNTAs showed large specific capacitance (Csp) of 693 F/g at a scan rate of 5 mV/s. The PPy/PANI DNTAs also exhibited good rate capability and high long-term cycle stability (less 8% loss of the maximum specific capacitance after 1000 cycles). The synergistic effects between PPy and PANI, the shape effects of nanotube arrays and double-walled nanostructures, and high utilization rate of electrode are crucial for the outstanding performance of PPy/PANI DNTAs. The large Csp, good rate capability, and high long-term cycle stability offered by the PPy/PANI DNTAs, make them promising candidate electrodes for high-performance supercapacitors.
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[Study of RON mediated invasion of Raji cell line and drug-target effects].
Zhonghua Xue Ye Xue Za Zhi
PUBLISHED: 12-04-2013
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To study the proto-oncogene RON mediated aggression of Raji cells and the inhibitory effects by monoclonal antibody Zt/f2 (2f2).
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The association between the Arg280His polymorphism in the XRCC1 gene and the risk of hematological malignancies.
Tumour Biol.
PUBLISHED: 08-17-2013
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The associations between the Arg280His polymorphism in X-ray repair cross-complementing gene 1 (XRCC1 gene) and hematological malignancies have been extensively investigated. However, the results were inconsistent. The objective of the current study was to investigate the associations between the Arg280His polymorphism in XRCC1 gene and the risk of hematological malignancies by meta-analysis. We searched PubMed, Embase, CNKI, Wanfang, and Weipu databases, covering all studies until 07 Aug 2013. Statistical analysis was performed by using the Revman4.2 software and the Stata10.0 software. A total of 2,650 cases and 3,856 controls in 12 case-control studies concerning the Arg280His polymorphism were included. The results suggested that the Arg280His polymorphism might not be associated with risk of hematological malignancies (OR?=?1.08, 95%CI?=?0.86-1.35, P?=?0.50). In the subgroup analyses by cancer types and ethnicity, no significant associations were found among different cancers or different ethnicities. The current meta-analysis indicated that the Arg280His polymorphism in the XRCC1 gene might not be a risk factor for hematological malignancies. In future, more large-scale case-control studies are needed to validate these results.
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[Effects of delayed first feeding on growth and survival of Hucho taimen larvae].
Ying Yong Sheng Tai Xue Bao
PUBLISHED: 08-01-2013
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This paper studied the effects of delaying first breeding Hucho taimen larvae for different days on the larvae growth, survival, and body size. Five treatments were installed, i. e. , feeding begins on the first eating day (control, S0) and on the 9th, 12th, 15th, and 18th days after the first eating day (S1 -S4) at 10.4-14.9 degree C, respectively. By the end of the experiment (36-day), the growth rate and initial feeding rate in S1 was higher than that in S0, and the overall mortality rate in S1 was lower, but the body size and mass in S1and S0 had no significant difference. Compared with S0, S2 had higher growth rate, initial feeding rate, total mortality, and self-mutilation mortality, the body mass was significantly lower, but the body size had less difference. S3 had higher first feeding rate, body size, total mortality, and self-mutilation mortality, but significantly lower body mass than S0, whereas the growth rate had less difference. In S4, the growth rate and body mass were lower, and the total mortality and self-mutilation mortality were higher than those in S0. It was suggested that under the same conditions, delaying first feeding for 9 days would induce H. taimen larvae presenting "completely compensatory growth", and this feeding way could be applied for the culture of H. taimen larvae in their initial feeding period.
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An endovascular treatment of a thoracic aortic injury caused by a misplaced pedicle screw: Case report and review of the literature.
J. Formos. Med. Assoc.
PUBLISHED: 07-15-2013
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Aortic injuries are devastating complications after spinal surgery. We here would like to share our experience with a successful endovascular treatment of an iatrogenic thoracic aortic injury caused by misplaced pedicle screw after spinal surgery.
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The Complete mitochondrial genome of Spinibarbus denticulatus (Oshima).
Mitochondrial DNA
PUBLISHED: 07-10-2013
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Abstract Spinibarbus denticulatus (Oshima) is a rare and commercial fish. The complete mitochondrial genome sequence of S. denticulatus will help us to study the genetic of conversation, such as the genetic diversity and genetic structure, and provides the basis for the study in evolution. In this paper, the complete mitochondrial genome of S. denticulatus was determined to be 16,549 bp in length by Sanger sequencing technology. Thirteen protein-coding genes, 22 tRNA genes and 2 ribosomal genes were characterized. We also analyzed the structure of control region, 6 CSBs (CSB-1, CSB-2, CSB-3, CSB-D, CSB-E and CSB-F) and 1 TAS were identified, the control region also included an AT unit tandem repeat with 17 repeat times.
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Design of Pd/PANI/Pd sandwich-structured nanotube array catalysts with special shape effects and synergistic effects for ethanol electrooxidation.
J. Am. Chem. Soc.
PUBLISHED: 07-09-2013
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Low cost, high activity, and long-term durability are the main requirements for commercializing fuel cell electrocatalysts. Despite tremendous efforts, developing non-Pt anode electrocatalysts with high activity and long-term durability at low cost remains a significant technical challenge. Here we report a new type of hybrid Pd/PANI/Pd sandwich-structured nanotube array (SNTA) to exploit shape effects and synergistic effects of Pd-PANI composites for the oxidation of small organic molecules for direct alcohol fuel cells. These synthesized Pd/PANI/Pd SNTAs exhibit significantly improved electrocatalytic activity and durability compared with Pd NTAs and commercial Pd/C catalysts. The unique SNTAs provide fast transport and short diffusion paths for electroactive species and high utilization rate of catalysts. Besides the merits of nanotube arrays, the improved electrocatalytic activity and durability are especially attributed to the special Pd/PANI/Pd sandwich-like nanostructures, which results in electron delocalization between Pd d orbitals and PANI ?-conjugated ligands and in electron transfer from Pd to PANI.
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Mitochondrial DNA sequence of Mongolian redfin (Chanodichthys mongolicus).
Mitochondrial DNA
PUBLISHED: 06-24-2013
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Abstract Monglian redfin is a kind of freshwater aquaculture species which has an important economic value in China. In this study, we report the complete sequence of mitochondrial genome of Monglian redfin. The complete mitochondrial genome sequence is determined to be 16,621 base pairs (bp) in length and contain 13 protein-coding gene (PCGs), 22 transfer RNA genes, large (rrnL) and small (rrnS) ribosomal RNA, and the non-coding control region. Its total A?+?T content is 55.98%. We also analyzed the structure of control region, six conserved sequence blocks (CSBs) (CSB-1, CSB-2, CSB-3, CSB-D, CSB-E and CSB-F) and one potential termination-associated sequence were detected and the control region also included a 2-bp tandem repeat with eight repeat times.
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[Calculation and simulation on infrared radiation of hot jet from engine].
Guang Pu Xue Yu Guang Pu Fen Xi
PUBLISHED: 04-17-2013
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Spectral distribution of infrared radiation from plume by the method to calculate infrared radiance of the gaze direction in small sight field was calculated. Based on numerical value and form of radiative transfer equation, infrared radiant intensity of the gaze direction was calculated using the Malkmus statistical narrow-band model and CG approach. Flow field and mole fraction distribution were simulated using a FLUENT computational fluid dynamic (CFD) software. Infrared imaging simulation model of hot jet was established. The hot jets infrared images of liquid rocket engine were generated. The results demonstrate that the method can detect well-resolved fine structure of flow field. And the model is also applicable to calculation and simulation on infrared radiation of hot jet from engine.
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Electrochemical synthesis of nanostructured materials for electrochemical energy conversion and storage.
Nanoscale
PUBLISHED: 04-16-2013
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Electrochemical synthesis represents a highly efficient method for the fabrication of nanostructured energy materials, and various nanostructures, such as nanorods, nanowires, nanotubes, nanosheets, dendritic nanostructures, and composite nanostructures, can be easily fabricated with advantages of low cost, low synthetic temperature, high purity, simplicity, and environmental friendliness. The electrochemical synthesis, characterization, and application of electrochemical energy nanomaterials have advanced greatly in the past few decades, allowing an increasing understanding of nanostructure-property-performance relationships. Herein, we highlight some recent progress in the electrochemical synthesis of electrochemical energy materials with the assistance of additives and templates in solution or grafted onto metal or conductive polymer supports, with special attention to the effects on surface morphologies, structures and, more importantly, electrochemical performance. The methodology for preparing novel electrochemical energy nanomaterials and their potential applications has been summarized. Finally, we outline our personal perspectives on the electrochemical synthesis and applications of electrochemical energy nanomaterials.
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Dynamically expressed microRNA-15b modulates the activities of CD8+ T lymphocytes in mice with Lewis lung carcinoma.
J Transl Med
PUBLISHED: 03-13-2013
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CD8+ T cells are key members of adaptive immunity against tumorigenesis. As subset of CD8+ T cells, effector T cells (Te) and memory T cells (Tm) have different biological activities. The former can kill tumor cells but come into apoptosis in a certain period and the latter is static with the ability of self-renewal. Previous studies showed that microRNAs (miRNA) played critical roles in regulating adaptive immunity. This study aimed to identify the different expression of miRNAs between Te and Tm cells in tumor-bearing mice and to sort out the target miRNAs which can be regulated to improve anti-tumor activities of CD8+ T cells.
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Controllable template-assisted electrodeposition of single- and multi-walled nanotube arrays for electrochemical energy storage.
Sci Rep
PUBLISHED: 02-04-2013
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Here we explored a novel ZnO nanorod array template-assisted electrodeposition route to synthesize large-scale single-walled polypyrrole (PPy) nanotube arrays (NTAs) and multi-walled MnO(2)/PPy/MnO(2) NTAs. The structures of nanotubes, such as external and inner diameters, wall thicknesses, and lengths, can be well controlled by adjusting the diameters and lengths of ZnO nanorods and deposition time. The synthesized hybrid MnO(2)/PPy/MnO(2) triple-walled nanotube arrays (TNTAs) as electrodes showed high supercapacitive perporties, excellent long-term cycling stability, and high energy and power densities. The PPy layers in MnO(2)/PPy/MnO(2) TNTAs provide reliable electrical connections to MnO(2) shells and uniquely serve as highly conductive cores to support the redox reactions in the active two-double MnO(2) shells with highly electrolytic accessible surface area. The fabricated multi-walled NTAs allow high efficient utilization of electrode materials with facilitated transports of ions and electrons. The outstanding performance makes MnO(2)/PPy/MnO(2) TNTAs promising candidates for supercapacitor electrodes.
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Hierarchical Pd-Sn alloy nanosheet dendrites: an economical and highly active catalyst for ethanol electrooxidation.
Sci Rep
PUBLISHED: 01-11-2013
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Hierarchical alloy nanosheet dendrites (ANSDs) are highly favorable for superior catalytic performance and efficient utilization of catalyst because of the special characteristics of alloys, nanosheets, and dendritic nanostructures. In this paper, we demonstrate for the first time a facile and efficient electrodeposition approach for the controllable synthesis of Pd-Sn ANSDs with high surface area. These synthesized Pd-Sn ANSDs exhibit high electrocatalytic activity and superior long-term cycle stability toward ethanol oxidation in alkaline media. The enhanced electrocataytic activity of Pd-Sn ANSDs may be attributed to Pd-Sn alloys, nanosheet dendrite induced promotional effect, large number of active sites on dendrite surface, large surface area, and good electrical contact with the base electrode. Because of the simple implement and high flexibility, the proposed approach can be considered as a general and powerful strategy to synthesize the alloy electrocatalysts with high surface areas and open dendritic nanostructures.
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West nile virus infection in Xinjiang, China.
Vector Borne Zoonotic Dis.
PUBLISHED: 01-05-2013
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An outbreak of fever and meningitis/encephalitis occurred in Xinjiang, China, from August 5 to September 3, 2004. In preliminary diagnostic testing, several cerebrospinal fluid (CSF) and serum samples showed positive immunoglobulin M (IgM) antibody to Japanese encephalitis virus. Here, the CSF and serum samples of 6 cases collected at that time were tested by immunofluorescence assay (IFA), enzyme-linked immunosorbent assay (ELISA), and plaque reduction neutralization assay (PRNT) for the existence of IgM antibody or neutralization antibody against West Nile virus (WNV) or other arboviruses. The results demonstrate the evidence of West Nile infection in Xinjiang, China.
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[Analysis of genetic diversity on 9 wild stocks of taimen (Hucho taimen) by microsatellite markers].
Zool. Res.
PUBLISHED: 12-21-2011
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Taimen (Hucho taimen) is a native fish species in China and it is in the state of endangerment. To explain clearly the genetic diversity and genetic structure, 9 wild populations of taimen were investigated using 20 microsatellite markers. The results showed that their observed heterozygosity ranged from 0.0994 to 0.8882, the expected heterozygosity varied from 0.2005 to 0.8759, and the range of PIC index was from 0.3432 to 0.5261 while population from Huma River had low genetic diversity. Fst of matching group ranged from 0.0246 to 0.2333 (P <0.0001)and Nm varied among 0.8216 to 9.9292, which indicated that the genetic differentiation was remarkable among populations.The half/full-sib family tests detected a proportion of half/full-sib family groups varying among 27.78% to 90.91%, showing a high inbred pressure and a risk of bottlenecks experienced by most groups. The AMOVA results showed that the global Fst was 0.1081; the clustering result showed that individuals from Beiji tributary of Heilongjiang River clustered as one clade, all individuals from Huma River and Wusuli River clustered as one clade and all individuals from the upper reaches of the Heilongjiang River clustered as another clade. All these results indicated that the decrease of taimen resource has affected the gene exchange among their populations. In order to achieve full protection of taimen germplasm resources, we should put an end to the destructive fishing for taimen and promotegene exchange among their populations.
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Super-large dendrites composed of trigonal PbO2 nanoplates with enhanced performances for electrochemical devices.
Chem. Commun. (Camb.)
PUBLISHED: 12-16-2011
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Super-large dendrites composed of trigonal PbO(2) nanoplates were successfully synthesized by electrodeposition and tested as potential materials for electrochemical device applications.
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[Progress in zinc finger nuclease engineering for targeted genome modification].
Yi Chuan
PUBLISHED: 11-05-2011
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Zinc finger nuclease (ZFN) is an artificially engineered hybrid protein that contains a zinc finger protein (ZFP) domain and a Fok I endonuclease cleavage domain. It has recently emerged as a powerful molecular tool for targeted genome modifications. ZFNs recognize and bind to specific DNA sequences to generate a double-strand break (DSB) by its nuclease activity. Based on this finding, various genetic methods, including gene targeting (gene disruption), gene addition, gene correction etc., are being designed to manipulate the genomes of different species at specific loci. One particular advantage of this new technique is its broad applications, which can be employed to generate desirable inheritable mutations both at the organismal level and at the cellular level. Here, we review the recent progress and prospects of ZFN technology. This article focused on the mechanism of how it works, currently available target assessment, ZFP library construction and screening methods, target modification strategies, as well as a collection of specie and genes that have been successfully modified by ZFN. This review will provide a useful reference for researchers who are interested in applying this new technique in their studies.
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A versatile Tb(III) complex for picosecond filamentation, a transparent thin film and a supramolecular gel.
Photochem. Photobiol. Sci.
PUBLISHED: 08-30-2011
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The first complex picosecond filament, namely a filament of terbium(III) p-hydroxybenzoate, is observed. The filament is the only example of Ln(III) ion two-photon absorption in a complex. A transparent, colorless and mechanically robust thin film, as well as a supramolecular gel, of this complex are prepared in a facile manner and exhibit strong luminescence. The thin film is characterized in detail by XRD, SEM, UV-vis, luminescence spectroscopy and lifetime.
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[Isolation and identification of cancer stem cells from primary human ovarian cancer tissues].
Zhonghua Zhong Liu Za Zhi
PUBLISHED: 08-17-2011
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To isolate and identify the cancer stem cells from primary human ovarian cancer tissues.
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[Biological and phylogenetic analysis of first isolate of Tahyna virus in China].
Bing Du Xue Bao
PUBLISHED: 05-03-2011
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In 2006, the first Chinese Tahyna virus isolate (XJ0625) was obtained in Xinjiang province and human infection were found in the same region. In this study, cell culture, animal experiments, electron microscopy, immunofluorescence assay and cross neutralization tests were performed to see the cell susceptibility, animal pathogenicity, morphology and antigenic and other biological characteristics of XJ0625. In addition, molecular biology software was used to analyze the characteristics of molecular evolution. The results showed that BHK-21 cell line was susceptible to XJ0625 and the virus was lethal to suckling mice when injected by intracranial ways. Similar to the other Bunyavirus, Tahyna virus is spherical enveloped virus under electron microscopy. XJ0625 infected cells showed strong fluorescent signal and could be neutralized by immune asities fluid with immnity to protype Tahyna virus Bardos 92. The sequence of the S and M segments showed 91.8% and 81.9% homology with Bardos 92.
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Inhibition of inflammatory mediators and related signaling pathways by macrophage-stimulating protein in rheumatoid arthritis synovial fibroblasts.
Inflamm. Res.
PUBLISHED: 04-11-2011
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To evaluate the mechanism of macrophage-stimulating protein (MSP)-mediated inhibition of inflammatory cytokine and chemokine production in rheumatoid arthritis synovial fibroblasts (RASF).
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EphB3 is overexpressed in non-small-cell lung cancer and promotes tumor metastasis by enhancing cell survival and migration.
Cancer Res.
PUBLISHED: 01-25-2011
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Eph receptors, the largest subfamily of transmembrane tyrosine kinase receptors, have been increasingly implicated in various physiologic and pathologic processes, and the roles of the Eph family members during tumorigenesis have recently attracted growing attention. Until now, research on EphB3 function in cancer is limited to focusing on tumor suppression by EphB receptors in colorectal cancer. However, its function in other types of cancer remains poorly investigated. In this study, we explored the function of EphB3 in non-small-cell lung cancer (NSCLC). We found that the expression of EphB3 was significantly upregulated in clinical samples and cell lines, and the expression level correlated with the patient pathologic characteristics, including tumor size, differentiation, and metastasis. Overexpression of EphB3 in NSCLC cell lines accelerated cell growth and migration and promoted tumorigenicity in xenografts in a kinase-independent manner. In contrast, downregulation of EphB3 inhibited cell proliferation and migration and suppressed in vivo tumor growth and metastasis. Furthermore, we showed that silencing of EphB3 inhibited cell growth by reducing DNA synthesis and caspase-8-mediated apoptosis and suppressed cell migration by increasing accumulation of focal adhesion formation. Taken together, our findings suggest that EphB3 provides critical support to the development and progression of NSCLC by stimulating cell growth, migration, and survival, thereby implicating EphB3 as a potential therapeutic target in NSCLC.
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Hematoma volume affects the accuracy of computed tomographic angiography spot sign in predicting hematoma expansion after acute intracerebral hemorrhage.
Eur. Neurol.
PUBLISHED: 01-10-2011
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We hypothesized that the presence of tiny, enhancing foci (spot sign) within acute hematomas is associated with hematoma expansion.
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Preclinical transplantation and safety of HS/PCs expanded from human umbilical cord blood.
World J Stem Cells
PUBLISHED: 01-05-2011
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To expand hematopoietic/progenitor stem cells (HS/PCs) from umbilical cord blood (UCB) and prepare the HS/PC product, and analyze preclinical transplantation and safety of HS/PC product.
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Facile electrochemical synthesis of hexagonal Cu2O nanotube arrays and their application.
Inorg Chem
PUBLISHED: 12-23-2010
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Large-scale and highly oriented single-crystalline hexagonal Cu(2)O nanotube arrays have been successfully synthesized using a two-step solution approach, which involves the electrodeposition of oriented Cu(2)O nanorods and a subsequent dissolution technique along the c axis to form a tubular structure. Herein, NH(4)Cl was found to be an effectual additive, and it can successfully realize the dissolution process of Cu(2)O from nanorods to nanotubes. The dissolution mechanism of Cu(2)O from nanorods to nanotubes was illustrated in detail. These prepared Cu(2)O nanotube arrays were characterized by SEM, EDS, XRD, XPS, and TEM. The photoluminescence (PL) spectrum of Cu(2)O nanotube arrays was also measured, and it shows there is a greater fraction of copper or oxygen vacancies in these prepared Cu(2)O nanotubes. Finally, the applications of Cu(2)O nanotube arrays for gas sensors were investigated in this paper.
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[Effect of prolonged infusion of propofol on the liver mitochondria respiratory function in rabbits].
Sichuan Da Xue Xue Bao Yi Xue Ban
PUBLISHED: 12-01-2010
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To investigate the changes of respiratory function of liver mitochondria in rabbits induced by the general anesthetic propofol.
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Tuning magnetic properties of CeO2 by Fe doping via an electrochemical deposition route.
Chemphyschem
PUBLISHED: 09-22-2010
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Herein Ce(1-x)Fe(x)O(2-?) nanocomposites were investigated for dilute magnetic semiconductor (DMS) properties. Ce(1-x)Fe(x)O(2-?) nanospheres and porous nanostructures with high surface areas have been successfully prepared by electrochemical deposition at room temperature and atmospheric pressure. The structures and morphologies of Ce(1-x)Fe(x)O(2-?) deposits were characterized by X-ray diffraction (XRD), scanning electron microscopy (SEM), transmission electron microscopy (TEM), and N(2) adsorption-desorption techniques. The magnetic properties of the prepared Ce(1-x)Fe(x)O(2-?) nanospheres and porous nanostructures were studied, and they showed room-temperature ferromagnetism and giant magnetic moments. In addition, the effects of morphologies and compositions on the magnetic properties of Ce(1-x)Fe(x)O(2-?) deposits were studied.
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[Applicability of microsatellite DNA markers to the parental identification of Hucho taimen (Pallas)].
Zool. Res.
PUBLISHED: 08-27-2010
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Using seven pairs of microsatellite markers we studied the relationship between the size of the the candidate groups and the microsatellite paternity testing accuracy by separate breed or the microsatellite paternity appraisal ability by mixed breed. By computer software analysis, the appraisal ability decreased with the candidate groups increase. For the 81 putative parents the microsatellite paternity testing accuracy was 80% and the microsatellite paternity appraisal ability was 78.9%; for the 9 putative parents, the testing accuracy was 93.3% and the appraisal ability was 92.2%. The results indicated that the microsatellite DNA markers can be used for the parentage determination of Hucho taimen Pallas.
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Synthesis of hierarchical rippled Bi(2)O(3) nanobelts for supercapacitor applications.
Chem. Commun. (Camb.)
PUBLISHED: 06-07-2010
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Hierarchical rippled Bi(2)O(3) nanobelts were successfully synthesized by an electrodeposition route and tested as promising materials for supercapacitor applications.
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An outbreak of the desert sub-type of zoonotic visceral leishmaniasis in Jiashi, Xinjiang Uygur Autonomous Region, Peoples Republic of China.
Parasitol. Int.
PUBLISHED: 04-11-2010
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Few outbreaks of the desert sub-type of zoonotic visceral leishmaniasis (VL) have been described worldwide. In 2008, the incidence rate of VL in Jiashi County, Xinjiang Uygur Autonomous Region in the western part of the Peoples Republic of China, increased more than twenty-folds compared to the average annual incidence rate. The majority of the cases (96.6%) occurred among <2 year-old infants. For the first time in the desert area of Xinjiang, the parasites were isolated from bone marrow aspirates, using the NNN medium culture approach. The genetic analysis of the ITS-1 nucleotide sequence indicated that three isolates from eastern Jiashi County were genetically closely related and belonged to the Leishmaniainfantum group. However, they differed from an isolate from Kashi city which was classified as a member of the Leishmaniadonovani group.
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Facile electrochemical synthesis of single crystalline CeO2 octahedrons and their optical properties.
Langmuir
PUBLISHED: 01-28-2010
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We developed a simple electrochemical process for the large-scale fabrication of single crystalline CeO(2) octahedrons and nanospheres from DMSO aqueous solution. The octahedrons with some structural defects have a size ranging from 200 to 300 nm. Moreover, highly crystalline CeO(2) nanospheres were also obtained via this electrochemical process based on the oriented attachment mechanism. The absorption edge of octahedrons and spheres shows a red-shift, and that of the octahedrons was near the visible region.
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Mesoporous MnO2/carbon aerogel composites as promising electrode materials for high-performance supercapacitors.
Langmuir
PUBLISHED: 01-14-2010
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MnO(2) as one of the most promising candidates for electrochemical supercapacitors has attracted much attention because of its superior electrochemical performance, low cost, and environmentally benign nature. In this Letter, we explored a novel route to prepare mesoporous MnO(2)/carbon aerogel composites by electrochemical deposition assisted by gas bubbles. The products were characterized by energy-dispersive spectrometry (EDS), X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), scanning electron microscopy (SEM), and transmission electron microscopy (TEM). The MnO(2) deposits are found to have high purity and have a mesoporous structure that will optimize the electronic and ionic conductivity to minimize the total resistance of the system and thereby maximize the performance characteristics of this material for use in supercapacitor electrodes. The results of nitrogen adsorption-desorption experiments and electrochemical measurements showed that these obtained mesoporous MnO(2)/carbon aerogel composites had a large specific surface area (120 m(2)/g), uniform pore-size distribution (around 5 nm), high specific capacitance (515.5 F/g), and good stability over 1000 cycles, which give these composites potential application as high-performance supercapacitor electrode materials.
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Ceria-terbia solid solution nanobelts with high catalytic activities for CO oxidation.
Chem. Commun. (Camb.)
PUBLISHED: 11-10-2009
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Ceria-terbia solid solution nanobelts were prepared by an electrochemical route and tested as catalysts of high activity for CO oxidation.
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[An epidemiological survey of visceral leishmaniasis in Hamangou coal mine area of Korla City, Xinjiang].
Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi
PUBLISHED: 10-27-2009
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To investigate the epidemiological status of visceral leishmaniasis in Hamangou coal mine area of Korla City of Xinjiang Uygur Autonomous Region.
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[beta-elemene enhances aclarubicin-induced apoptotic effect in HL-60 cells and its mechanism.].
Zhonghua Xue Ye Xue Za Zhi
PUBLISHED: 10-19-2009
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To explore the effects of beta-elemene combined with aclarubicin on the induction of HL-60 cell apoptosis and its mechanisms in antileukemia therapy.
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[Study on time-space clustering regarding the distribution of visceral leishmaniasis in Kashgar Region, Xinjiang].
Zhonghua Liu Xing Bing Xue Za Zhi
PUBLISHED: 07-02-2009
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To probe time-space clustering on the distribution of visceral leishmaniasis (VL) in Kashgar Region.
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[Isolation and primary identification of viruses in mosquitoes in the south of Xinjiang].
Zhonghua Yu Fang Yi Xue Za Zhi
PUBLISHED: 06-19-2009
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To isolate viruses from mosquitoes in the south of Xinjiang and identify these viruses primarily.
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[A new member of Brevidensovirus, 0507JS11 virus isolated from Culex mosquitoes collected in Xinjiang].
Zhonghua Yu Fang Yi Xue Za Zhi
PUBLISHED: 06-19-2009
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To probe the primary characteristic of 0507JS11 virus isolated from Culex sp. and determine the classification of 0507JS11 virus in taxonomy.
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[Investigation on echinococcosis in animals in Gannan Tibetan Autonomous Prefecture].
Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi
PUBLISHED: 05-23-2009
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To make an investigation on echinococcosis among animals in Gannan Tibetan Autonomous Prefecture.
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t(X;17) as the sole karyotypic anomaly in a case of M(3r) subtype of acute promyelocytic leukemia without RARalpha rearrangement.
Leuk. Res.
PUBLISHED: 04-19-2009
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We describe here a unique chromosomal abnormality found in a patient with M(3r) subtype of APL. Neither t(15;17) nor rearrangement of RARalpha was detected by routine R-banded chromosome as well as fluorescence in situ hybridization (FISH) analysis using PML/RARalpha dual-color dual-fusion translocation probe and RARalpha dual-color break apart rearrangement probe. Instead of the typical rearrangement between chromosomes 15 and 17, all cells analyzed had a translocation between X and 17 as the sole karyotypic anomaly. The translocation was conformed by whole chromosome painting (WCP) with painting probes of chromosomes X and 17. To our knowledge, this is the first documented APL with a novel translocation involving chromosomes X and 17 without RARalpha gene rearrangement.
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[Cordyceps sinensis polysaccharide enhances apoptosis of HL-60 cells induced by triptolide].
Zhejiang Da Xue Xue Bao Yi Xue Ban
PUBLISHED: 04-14-2009
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To investigate the effects of polysaccharide fraction of Cordyceps sinensis (PSCS) on triptolide (TPL)-induced apoptosis in the HL-60 cells and the involved molecular mechanism.
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[The laboratorial study about relation of balance between trestle and cervical spine of cervical type spondylosis patients].
Zhongguo Yi Liao Qi Xie Za Zhi
PUBLISHED: 03-02-2009
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When 85 cervical type spondylosis patients randomly lay on ones back and on ones right side the 8 different tresles and pillows. We survey indexes of the cervical spine anatomy, for example cervical arcs and angles of cervical spine and level line. We appraise relation of balance between different trestle or pillow and cervical spine. liquid needle-free injection, jet power, stagnation pressure
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Use of additives in the electrodeposition of nanostructured Eu3+/ZnO films for photoluminescent devices.
Langmuir
PUBLISHED: 02-10-2009
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Rare-earth ion-doped ZnO has been the focus of numerous investigations because of its unique optical properties and promising applications in optoelectronic devices. Here we presented a facile electrochemical deposition route for the controllable preparation of Eu3+/ZnO nanostructures on a large scale. The prepared Eu3+/ZnO deposits were characterized by scanning electron microscopy, energy dispersive spectroscopy, X-ray diffraction, selected area electron diffraction, and X-ray photoelectron spectroscopy. Herein, the growth mechanisms of Eu3+/ZnO nanosheets and nanorods were discussed. The formation process of Eu3+/ZnO foam-like nanostructures is illuminated in this paper. The room temperature photoluminescence properties of the Eu3+/ZnO foam-like nanostructures were investigated. The sharp 4f-4f transition emissions of Eu3+ can be directly observed at 593, 617, and 698 nm. An energy transfer between ZnO and Eu3+ is shown to occur under UV excitation.
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Tahyna virus and human infection, China.
Emerging Infect. Dis.
PUBLISHED: 02-06-2009
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In 2006, Tahyna virus was isolated from Culex spp. mosquitoes collected in Xinjiang, Peoples Republic of China. In 2007, to determine whether this virus was infecting humans, we tested serum from febrile patients. We found immunoglobulin (Ig) M and IgG against the virus, which suggests human infection in this region.
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Effects of isoflurane inhalation on the male reproductive system in rats.
Environ. Toxicol. Pharmacol.
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The 15-day intact adult male assay was used to evaluate effects of isoflurane on the testes and sexual hormones. Forty adult male Sprague-Dawley rats were divided into five groups exposed to air containing 0, 50, 300, 1800 or 10,800 ppm isoflurane. After the treatments, serum was collected for the hormones assay. The right testis was to be used for daily sperm production. The left testis was processed for histopathology and electron microscopy observation. Daily sperm productions were significantly decreased at doses of 300, 1800 and 10,800 ppm. Impaired seminiferous tubules were noted at doses of 300, 1800 and 10,800 ppm. Ultrastructural changes included nucleus agglutination of spermatocytes, big lipid drops and autophagosome in cytoplasm. The serum follicle-stimulating hormone and testosterone concentrations reduced significantly at doses of 1800 and 10,800 ppm. Isoflurane induced impairments of seminiferous tubules and spermatogenesis. The testicular damages caused by isoflurane can be related to the imbalances in the sexual hormones.
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Autocrine CCL5 signaling promotes invasion and migration of CD133+ ovarian cancer stem-like cells via NF-?B-mediated MMP-9 upregulation.
Stem Cells
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The concept of cancer stem cells (CSCs) proposes that solely CSCs are capable of generating tumor metastases. However, how CSCs maintain their invasion and migration abilities, the most important properties of metastatic cells, still remains elusive. Here we used CD133 to mark a specific population from human ovarian cancer cell line and ovarian cancer tissue and determined its hyperactivity in migration and invasion. Therefore, we labeled this population as cancer stem-like cells (CSLCs). In comparison to CD133- non-CSLCs, chemokine CCL5 and its receptors, CCR1, CCR3, and CCR5, were consistently upregulated in CSLCs, and most importantly, blocking of CCL5, CCR1, or CCR3 effectively inhibits the invasive capacity of CSLCs. Mechanistically, we identified that this enhanced invasiveness is mediated through nuclear factor ?B (NF-?B) activation and the consequently elevated MMP9 secretion. Our results suggested that the autocrine activation of CCR1 and CCR3 by CCL5 represents one of major mechanisms underlying the metastatic property of ovarian CSLCs.
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Simple automated radiosynthesis of 10-[11C]methoxy-20(S)-camptothecin and biodistribution in normal mice.
Appl Radiat Isot
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10-[(11)C]methoxy-20(S)-camptothecin was synthesized automatically. The radiochemical yield was 30%-50% (all calculated at EOB, n=20) with [(11)C]methyl triflate as a methylating agent. The radiochemical purity was greater than 96%, and the specific activity was 8.72±2.18 GBq/?moL at EOS. Biodistribution showed that 10-[(11)C]methoxy-20(S)-camptothecin is characterized by quick clearance from the blood and by significant uptake in the liver (5.72±1.92% ID/g at 15 min), intestines (2.43±0.38% ID/g at 15 min), and kidney (1.57±0.85% ID/g at 15 min). Micro-PET imaging clearly showed high radioactivity accumulation in liver, intestines and bladder, indicating that the primary modes of excretion of the radiotracer are through the hepatobiliary system and, to a lesser extent, through the renal system.
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MDM2 inhibitor Nutlin-3a suppresses proliferation and promotes apoptosis in osteosarcoma cells.
Acta Biochim. Biophys. Sin. (Shanghai)
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Restoring p53 activity by inhibiting the interaction between p53 and the mouse double minutes clone 2 (MDM2) offers an attractive approach to cancer therapy. Nutlin-3a is a small-molecule inhibitor that inhibits MDM2 binding to p53 and subsequent p53-dependent DNA damage signaling. In this study, we determined the efficacy of Nutlin-3a in inducing p53-mediated cell death in osteosarcoma (OS) cell lines both in vivo and in vitro. Targeted disruption of the p53-MDM2 interaction by Nutlin-3a stabilizes p53 and selectively activates the p53 pathway only in OS cells with wild-type p53, resulting in a pronounced anti-proliferative and cytotoxic effect due to G1 cell cycle arrest and apoptosis both in vitro and in vivo. p53 dependence of these alternative outcomes of Nutlin-3a treatment was shown by the abrogation of these effects when p53 was knocked-down by small interfering RNA. These data suggest that the disruption of p53-MDM2 interaction by Nutlin-3a might be beneficial for OS patients with MDM2 amplification and wt p53 status.
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Design and synthesis of MnO?/Mn/MnO? sandwich-structured nanotube arrays with high supercapacitive performance for electrochemical energy storage.
Nano Lett.
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We demonstrate the design and fabrication of novel nanoarchitectures of MnO(2)/Mn/MnO(2) sandwich-like nanotube arrays for supercapacitors. The crystalline metal Mn layers in the MnO(2)/Mn/MnO(2) sandwich-like nanotubes uniquely serve as highly conductive cores to support the redox active two-double MnO(2) shells with a highly electrolytic accessible surface area and provide reliable electrical connections to MnO(2) shells. The maximum specific capacitances of 937 F/g at a scan rate of 5 mV/s by cyclic voltammetry (CV) and 955 F/g at a current density of 1.5 A/g by chronopotentiometry were achieved for the MnO(2)/Mn/MnO(2) sandwich-like nanotube arrays in solution of 1.0 M Na(2)SO(4). The hybrid MnO(2)/Mn/MnO(2) sandwich-like nanotube arrays exhibited an excellent rate capability with a high specific energy of 45 Wh/kg and specific power of 23 kW/kg and excellent long-term cycling stability (less 5% loss of the maximum specific capacitance after 3000 cycles). The high specific capacitance and charge-discharge rates offered by such MnO(2)/Mn/MnO(2) sandwich-like nanotube arrays make them promising candidates for supercapacitor electrodes, combining high-energy densities with high levels of power delivery.
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Isolates of Liao ning virus from wild-caught mosquitoes in the Xinjiang province of China in 2005.
PLoS ONE
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Liao ning virus (LNV) is related to Banna virus, a known human-pathogen present in south-east Asia. Both viruses belong to the genus Seadornavirus, family Reoviridae. LNV causes lethal haemorrhage in experimentally infected mice. Twenty seven isolates of LNV were made from mosquitoes collected in different locations within the Xinjiang province of north-western China during 2005. These mosquitoes were caught in the accommodation of human patients with febrile manifestations, or in animal barns where sheep represent the main livestock species. The regions where LNV was isolated are affected by seasonal encephalitis, but are free of Japanese encephalitis (JE). Genome segment 10 (Seg-10) (encoding cell-attachment and serotype-determining protein VP10) and Seg-12 (encoding non-structural protein VP12) were sequenced for multiple LNV isolates. Phylogenetic analyses showed a less homogenous Seg-10 gene pool, as compared to segment 12. However, all of these isolates appear to belong to LNV type-1. These data suggest a relatively recent introduction of LNV into Xinjiang province, with substitution rates for LNV Seg-10 and Seg-12, respectively, of 2.29×10(-4) and 1.57×10(-4) substitutions/nt/year. These substitution rates are similar to those estimated for other dsRNA viruses. Our data indicate that the history of LNV is characterized by a lack of demographic fluctuations. However, a decline in the LNV population in the late 1980s-early 1990s, was indicated by data for both Seg-10 and Seg-12. Data also suggest a beginning of an expansion in the late 1990s as inferred from Seg-12 skyline plot.
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Porous Ni@Pt core-shell nanotube array electrocatalyst with high activity and stability for methanol oxidation.
Chemistry
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Bimetallic core-shell nanostructures are emerging as more important materials than monometallic nanostructures, and have much more interesting potential applications in various fields, including catalysis and electronics. In this work, we demonstrate the facile synthesis of core-shell nanotube array catalysts consisting of Pt thin layers as the shells and Ni nanotubes as the cores. The porous Ni@Pt core-shell nanotube arrays were fabricated by ZnO nanorod-array template-assisted electrodeposition, and they represent a new class of nanostructures with a high electrochemically active surface area of 50.08 m(2) ?(g Pt)(-1), which is close to the value of 59.44 m(2) ?(g Pt)(-1) for commercial Pt/C catalysts. The porous Ni@Pt core-shell nanotube arrays also show markedly enhanced electrocatalytic activity and stability for methanol oxidation compared with the commercial Pt/C catalysts. The attractive performances exhibited by these prepared porous Ni@Pt core-shell nanotube arrays make them promising candidates as future high-performance catalysts for methanol electrooxidation. The facile method described herein is suitable for large-scale, low-cost production, and significantly lowers the Pt loading, and thus, the cost of the catalysts.
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Porous Pt-Ni-P composite nanotube arrays: highly electroactive and durable catalysts for methanol electrooxidation.
J. Am. Chem. Soc.
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Porous Pt-Ni-P composite nanotube arrays (NTAs) on a conductive substrate in good solid contact are successfully synthesized via template-assisted electrodeposition and show high electrochemical activity and long-term stability for methanol electrooxidation. Hollow nanotubular structures, porous nanostructures, and synergistic electronic effects of various elements contribute to the high electrocatalytic performance of porous Pt-Ni-P composite NTA electrocatalysts.
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JoVE Visualize is a tool created to match the last 5 years of PubMed publications to methods in JoVE's video library.

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In developing our video relationships, we compare around 5 million PubMed articles to our library of over 4,500 methods videos. In some cases the language used in the PubMed abstracts makes matching that content to a JoVE video difficult. In other cases, there happens not to be any content in our video library that is relevant to the topic of a given abstract. In these cases, our algorithms are trying their best to display videos with relevant content, which can sometimes result in matched videos with only a slight relation.