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Find video protocols related to scientific articles indexed in Pubmed.
Space-bound optical source for satellite-ground decoy-state quantum key distribution.
Opt Express
PUBLISHED: 11-18-2014
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Satellite-ground quantum key distribution has embarked on the stage of engineering implementation, and a global quantum-secured network is imminent in the foreseeable future. As one payload of the quantum-science satellite which will be ready before the end of 2015, we report our recent work of the space-bound decoy-state optical source. Specialized 850 nm laser diodes have been manufactured and the integrated optical source has gotten accomplished based on these LDs. The weak coherent pulses produced by our optical source feature a high clock rate of 100 MHz, intensity stability of 99.5%, high polarization fidelity of 99.7% and phase randomization. A series of space environment tests have been conducted to verify the optical source's performance and the results are satisfactory. The emulated final secure keys are about 120 kbits during one usable pass of the low Earth orbit satellite. This work takes a significant step forward towards satellite-ground QKD and the global quantum-secured network.
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Erratum to: Identification of a novel mutation in a Chinese family with Nance-Horan syndrome by whole exome sequencing.
J Zhejiang Univ Sci B
PUBLISHED: 11-05-2014
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Erratum to: J Zhejiang Univ-Sci B (Biomed & Biotechnol) 2014 Aug.; 15(8):727-734.
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A549 cell proliferation inhibited by RNAi mediated silencing of the Nrf2 gene.
Biomed Mater Eng
PUBLISHED: 09-18-2014
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Non-small-cell lung cancer (NSCLC), the most common type of lung cancers, is resistant to initial chemotherapy intrinsically. The expressions of xenobiotic metabolism genes, antioxidants, and drug efflux proteins are increased in NSCLC. In addition, a redox-sensitive transcription factor named Nrf2 regulates the drug resistance via the expression of electrophile, oxidants detoxification enzymes and efflux mechanism. As was detected by real-time PCR, inhibiting Nrf2 expression through the transfection of shRNA plasmids in A549 cells significantly inhibits the expressions of glutathione pathway genes, antioxidants and multidrug resistance proteins. Using biochemical assays and free radical medical experiments in vitro, it was identified that the RNAi-mediated reduction of Nrf2 expression in lung cancer cells induces the generation of reactive oxygen species, decreases the level of reduced glutathione and results in an increase in the A549 cell proliferation inhibition rate. Thus, targeting Nrf2 activity in NSCLC could be a practical way to inhibit tumor growth and eliminate chemoresistance.
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Asymmetric synthesis of (3S,1'S)-3-(1-amino-2,2,2-trifluoroethyl)-1-(alkyl)-indolin-2-one derivatives by addition of (S)-N-t-butylsulfinyl-3,3,3-trifluoroacetaldimine to 1-(alkyl)-indolin-2-ones.
Org. Biomol. Chem.
PUBLISHED: 09-16-2014
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This paper presents the first study of the addition reactions between amide-derived nucleophiles and chiral CF3-containing N-sulfinyl-imines. We demonstrate that enolates of 1-(alkyl)-indolin-2-ones cleanly react with (S)-N-t-butylsulfinyl-3,3,3-trifluoroacetaldimine affording 3-(1-amino-2,2,2-trifluoroethyl)-1-(alkyl)-indolin-2-ones of (3S,1'S) absolute configuration as the major reaction products with synthetically meaningful diastereoselectivity and chemical yields. The reactions were shown to be of general practical application for preparation of various oxindole derivatives in a diastereomerically pure form.
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Generalized access to fluorinated ?-keto amino compounds through asymmetric additions of ?,?-difluoroenolates to CF3-sulfinylimine.
Org. Biomol. Chem.
PUBLISHED: 08-28-2014
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CF3-containing chiral imines readily react with ?,?-difluoroenolates affording a novel type of ?-keto-amino compounds featuring the R-CO-CF2-CH(NH2)-CF3 moiety. The reactions feature exceptional generality allowing preparation of various aromatic, hetero-aromatic and aliphatic derivatives in high yields and diastereoselectivity. The products are configurationally stable and can be transformed to more functionalized complex compounds.
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HIF2? is associated with poor prognosis and affects the expression levels of survivin and cyclin D1 in gastric carcinoma.
Int. J. Oncol.
PUBLISHED: 08-21-2014
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Hypoxia-inducible factor-2? (HIF2?) is a major determinant factor of invasion and metastasis in various tumors. It has been reported that HIF2? is overexpressed in many tumors, including gastric cancer. However, the roles of HIF2? in the progression of gastric cancer are still not clear. In this study, we first examined the levels of HIF2? in gastric cancer by using immunohistochemistry, western blot and real-time PCR analysis. The results showed that HIF2? was highly expressed in gastric cancers compared to non-neoplastic mucosa and significantly correlated with histologic grade, TNM stages and peritoneal dissemination. MTT and colony formation assay revealed HIF2? overexpression induced high proliferation in BGC823 cells and HIF2? knockdown significantly inhibited proliferation in SGC7901 cells. Furthermore, we demonstrated that HIF2? could promote migration and invasion in gastric cancer cells. The results of western blot and RT-PCR analysis indicated that Survivin, Cyclin D1, MMP2 and MMP9 are upregulated with HIF2? overexpression. Finally, similar roles of HIF2? also in vivo were demonstrated. Taken together, the present study suggested that HIF-2? was involved in proliferation, metastasis and invasion of gastric cancer cells, with the induction of Survivin, Cyclin D1, MMP2 and MMP9 expression.
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Pre-operative visual evaluation for implantable collamer lenses in highly myopic eyes.
Clin Exp Optom
PUBLISHED: 08-20-2014
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The aim of this study was to evaluate a clinical procedure based on trial rigid gas-permeable (RGP) contact lenses fitting to assess the real pre-operative visual acuity (VA) of highly myopic eyes undergoing surgery with an implantable collamer lens.
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Identification of a novel mutation in a Chinese family with Nance-Horan syndrome by whole exome sequencing.
J Zhejiang Univ Sci B
PUBLISHED: 08-06-2014
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Nance-Horan syndrome (NHS) is a rare X-linked disorder characterized by congenital nuclear cataracts, dental anomalies, and craniofacial dysmorphisms. Mental retardation was present in about 30% of the reported cases. The purpose of this study was to investigate the genetic and clinical features of NHS in a Chinese family.
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Asymmetric Friedel-Crafts reactions of N-tert-butylsulfinyl-3,3,3-trifluoroacetaldimines: general access to enantiomerically pure indoles containing a 1-amino-2,2,2-trifluoroethyl group.
J. Org. Chem.
PUBLISHED: 07-28-2014
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We have demonstrated that 3,3,3-trifluoroacetaldimine (S)-1 easily reacts with indole derivatives under Friedel-Crafts reactions to provide reliable and generalized access to biologically interesting compounds containing the CF3CH(NH2)- pharmacophoric group. The reactions proceed with high rates and generally excellent yields (>90%) and stereochemical outcomes (99:1 dr).
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Comparative studies of salinomycin-loaded nanoparticles prepared by nanoprecipitation and single emulsion method.
Nanoscale Res Lett
PUBLISHED: 07-15-2014
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To establish a satisfactory delivery system for the delivery of salinomycin (Sal), a novel, selective cancer stem cell inhibitor with prominent toxicity, gelatinase-responsive core-shell nanoparticles (NPs), were prepared by nanoprecipitation method (NR-NPs) and single emulsion method (SE-NPs). The gelatinase-responsive copolymer was prepared by carboxylation and double amination method. We studied the stability of NPs prepared by nanoprecipitation method with different proportions of F68 in aqueous phase to determine the best proportion used in our study. Then, the NPs were prepared by nanoprecipitation method with the best proportion of F68 and single emulsion method, and their physiochemical traits including morphology, particle size, zeta potential, drug loading content, stability, and in vitro release profiles were studied. The SE-NPs showed significant differences in particle size, drug loading content, stability, and in vitro release profiles compared to NR-NPs. The SE-NPs presented higher drug entrapment efficiency and superior stability than the NR-NPs. The drug release rate of SE-NPs was more sustainable than that of the NR-NPs, and in vivo experiment indicated that NPs could prominently reduce the toxicity of Sal. Our study demonstrates that the SE-NPs could be a satisfactory method for the preparation of gelatinase-responsive NPs for intelligent delivery of Sal.
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Highly efficient and generalized asymmetric synthesis of quaternary stereogenic carbon-containing ?-amino indanones/indanoles via Mannich-type additions between 1-indanones and N-tert-butanesulfinylketimines.
Org. Biomol. Chem.
PUBLISHED: 05-23-2014
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Here we report that, unlike other ketones, 1-indanone and acetophenone derived enolates undergo Mannich-type addition reactions with N-tert-butanesulfinyl ketimines with excellent yields (up to 98%) and diastereoselectivity (>99/1). The resulting compounds represent a new type of biologically relevant ?-aminoketone derivative bearing quaternary stereogenic carbon, which could be further converted into the corresponding ?-amino ketones and ?-amino alcohols, possessing three consecutive stereogenic centres.
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Increased 14-3-3? expression in the multidrug-resistant leukemia cell line HL-60/VCR as compared to the parental line mediates cell growth and apoptosis in part through modification of gene expression.
Acta Haematol.
PUBLISHED: 03-06-2014
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Acute myeloid leukemia (AML) recurrence is largely a result of multidrug resistance (MDR). We aimed to examine the role of 14-3-3? in AML chemosensitivity using HL-60 and vincristine-resistant HL-60/VCR cells.
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Symmetry-protected topological phases from decorated domain walls.
Nat Commun
PUBLISHED: 02-25-2014
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Symmetry-protected topological phases generalize the notion of topological insulators to strongly interacting systems of bosons or fermions. A sophisticated group cohomology approach has been used to classify bosonic symmetry-protected topological phases, which however does not transparently predict their properties. Here we provide a physical picture that leads to an intuitive understanding of a large class of symmetry-protected topological phases in d=1,2,3 dimensions. Such a picture allows us to construct explicit models for the symmetry-protected topological phases, write down ground state wave function and discover topological properties of symmetry defects both in the bulk and on the edge of the system. We consider symmetries that include a Z2 subgroup, which allows us to define domain walls. While the usual disordered phase is obtained by proliferating domain walls, we show that symmetry-protected topological phases are realized when these domain walls are decorated, that is, are themselves symmetry-protected topological phases in one lower dimension. This construction works both for unitary Z2 and anti-unitary time reversal symmetry.
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Concise and scalable asymmetric synthesis of 5-(1-amino-2,2,2-trifluoroethyl)thiazolo[3,2-b][1,2,4]triazoles.
Org. Biomol. Chem.
PUBLISHED: 02-20-2014
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This study describes asymmetric Mannich-type additions between C-5 lithiated thiazolo[3,2-b][1,2,4]triazoles and enantiomerically pure (SS)-N-tert-butanesulfinyl-(3,3,3)-trifluoroacetaldimine. Under the optimized conditions, these reactions proceed with good (up to 78%) chemical yields and virtually complete (98?:?2 to >99?:?1 dr) diastereoselectivity. The same stereochemical outcome was obtained using 1.05 g scale of the starting (3,3,3)-trifluoroacetaldimine. The method developed in this work provides concise and generalized access to thiazolo[3,2-b][1,2,4]triazoles containing a chiral (trifluoro)ethylamine group.
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Low-dimensional carboxylate-bridged Gd(III) complexes for magnetic refrigeration.
Chem Asian J
PUBLISHED: 02-12-2014
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Four carboxylate-bridged Gd(III) complexes (1-4) with 1D/2D structures have been synthesized by using the hydrothermal reaction of Gd2O3 with various carboxylate ligands. Compounds 1 and 2 contained the same [2n] Gd(III)-OH ladders, but with different crystallographically independent Gd(III) ions, whilst the structures of compounds 3 and 4 were composed of [Gd4(?3-OH)2(piv)8(H2O)2](2+) units and 1D ladder Gd(III) chains, respectively. Antiferromagnetic interactions occurred in compounds 1-3, owing to their small Gd-O-Gd angles, whereas ferromagnetic coupling occurred in compound 4, in which the Gd-O-Gd angles were larger. These complexes exhibited a distinct magnetocaloric effect (MCE), which was affected by their different magnetic densities and exchange interactions. Among these compounds, complex 4 presented the largest MCE (-?S(m)(max)=43.6?J?kg(-1) K(-1)), the lowest M(w)/N(Gd) ratio (the highest magnetic density), and weak ferromagnetic coupling. Therefore, a lower M(w)/N(Gd) ratio and weaker exchange interactions (a smaller absolute value of ?) between Gd(III) ions resulted in a larger MCE for the Gd(III) complexes.
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RAD18 mediates resistance to ionizing radiation in human glioma cells.
Biochem. Biophys. Res. Commun.
PUBLISHED: 01-27-2014
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Radioresistance remains a major challenge in the treatment of glioblastoma multiforme (GBM). RAD18 a central regulator of translesion DNA synthesis (TLS), has been shown to play an important role in regulating genomic stability and DNA damage response. In the present study, we investigate the relationship between RAD18 and resistance to ionizing radiation (IR) and examined the expression levels of RAD18 in primary and recurrent GBM specimens. Our results showed that RAD18 is an important mediator of the IR-induced resistance in GBM. The expression level of RAD18 in glioma cells correlates with their resistance to IR. Ectopic expression of RAD18 in RAD18-low A172 glioma cells confers significant resistance to IR treatment. Conversely, depletion of endogenous RAD18 in RAD18-high glioma cells sensitized these cells to IR treatment. Moreover, RAD18 overexpression confers resistance to IR-mediated apoptosis in RAD18-low A172 glioma cells, whereas cells deficient in RAD18 exhibit increased apoptosis induced by IR. Furthermore, knockdown of RAD18 in RAD18-high glioma cells disrupts HR-mediated repair, resulting in increased accumulation of DSB. In addition, clinical data indicated that RAD18 was significantly higher in recurrent GBM samples that were exposed to IR compared with the corresponding primary GBM samples. Collectively, our findings reveal that RAD18 may serve as a key mediator of the IR response and may function as a potential target for circumventing IR resistance in human GBM.
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Effects of white light-emitting diode (LED) light exposure with different correlated color temperatures (CCTs) on human lens epithelial cells in culture.
Photochem. Photobiol.
PUBLISHED: 01-21-2014
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Cataract is the major cause for legal blindness in the world. Oxidative stress on the lens epithelial cells (hLECs) is the most important factor in cataract formation. Cumulative light-exposure from widely used light-emitting diodes (LEDs) may pose a potential oxidative threat to the lens epithelium, due to the high-energy blue light component in the white-light emission from diodes. In the interest of perfecting biosafety standards for LED domestic lighting, this study analyzed the photobiological effect of white LED light with different correlated color temperatures (CCTs) on cultured hLECs. The hLECs were cultured and cumulatively exposed to multichromatic white LED light with CCTs of 2954, 5624, and 7378 K. Cell viability of hLECs was measured by Cell Counting Kit-8 (CCK-8) assay. DNA damage was determined by alkaline comet assay. Intracellular reactive oxygen species (ROS) generation, cell cycle, and apoptosis were quantified by flow cytometry. Compared with 2954 and 5624 K LED light, LED light having a CCT of 7378 K caused overproduction of intracellular ROS and severe DNA damage, which triggered G2 /M arrest and apoptosis. These results indicate that white LEDs with a high CCT could cause significant photobiological damage to hLECs.
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Root proteome of rice studied by iTRAQ provides integrated insight into aluminum stress tolerance mechanisms in plants.
J Proteomics
PUBLISHED: 01-09-2014
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One of the major limitations to crop growth on acid soils is the prevalence of soluble aluminum ions (Al(3+)). Rice (Oryza sativa L.) has been reported to be highly Al tolerant; however, large-scale proteomic data of rice in response to Al(3+) are still very scanty. Here, we used an iTRAQ-based quantitative proteomics approach for comparative analysis of the expression profiles of proteins in rice roots in response to Al(3+) at an early phase. A total of 700 distinct proteins (homologous proteins grouped together) with >95% confidence were identified. Among them, 106 proteins were differentially expressed upon Al(3+) toxicity in sensitive and tolerant cultivars. Bioinformatics analysis indicated that glycolysis/gluconeogenesis was the most significantly up-regulated biochemical process in response to excess Al(3+). The mRNA levels of eight proteins mapped in the glycolysis/gluconeogenesis were further analyzed by qPCR and the expression levels of all the eight genes were higher in tolerant cultivar than in sensitive cultivar, suggesting that these compounds may promote Al tolerance by modulating the production of available energy. Although the exact roles of these putative tolerance proteins remain to be examined, our data lead to a better understanding of the Al tolerance mechanisms in rice plants through the proteomics approach.
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Asymmetric Mannich reactions of imidazo[2,1-b]thiazole-derived nucleophiles with (S(S))-N-tert-butanesulfinyl (3,3,3)-trifluoroacetaldimine.
Org. Biomol. Chem.
PUBLISHED: 10-29-2013
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Asymmetric Mannich reactions of imidazo[2,1-b]thiazole-derived nucleophiles with (SS)-N-tert-butanesulfinyl (3,3,3)-trifluoroacetaldimine were found to proceed with reasonably good yields (55%-79%) and exceptionally high stereoselectivity (>99?:?1 dr). This method presents a general approach for the preparation of a new type of biologically relevant compounds containing pharmacophoric imidazo[2,1-b]thiazole and (trifluoro)ethylamine groups.
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Asymmetric synthesis of ?-alkenyl homoallylic primary amines via 1,2-addition of Grignard reagent to ?,?-unsaturated phosphonyl imines.
RSC Adv
PUBLISHED: 10-26-2013
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A series of chiral N-phosphonyl protected ?-alkenyl homoallylic primary amines were synthesized by asymmetric addition of allylmagnesium bromide Grignard reagent towards chiral ?,?-unsaturated imines. Only 1,2-adduct was obtained for all the imines with good yields and excellent diastereoselectivities. The chiral auxiliary could be easily removed under simple conditions, giving free multiple functionalized primary amines.
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[Fluoroquinolone associated myasthenia gravis exacerbation: clinical analysis of 9 cases].
Zhonghua Yi Xue Za Zhi
PUBLISHED: 09-14-2013
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To explore the characteristics of acute exacerbations of myasthenia gravis after fluoroquinolone exposure.
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[Inhibitory effect of 14-3-3? on the proliferation of HL-60 cells and HL-60/VCR cells].
Zhongguo Shi Yan Xue Ye Xue Za Zhi
PUBLISHED: 09-04-2013
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This study was aimed to investigate the expression and role of 14-3-3? in the AML cell lines: sensitive HL-60 and drug-resistant HL-60/VCR cells. Semi-quantitative RT-PCR and Western blot were respectively used to examine the expression of mdr1 mRNA and Pgp in AML cell lines to validate the results of microarray. Western blot was performed to investigate the expression of Pgp, 14-3-3?, and anti-apoptosic protein BCL-2, MCL-1 proteins. Immunofluorescence assay was used to detect the subcellular location of 14-3-3? protein in HL-60 and HL-60/VCR cells by laser scanning confocal microscopy. Transduction with siRNA was used to silence 14-3-3? in AML cell lines. Cell count method and flow cytometry of cell cycle were used to analyze the changes of growth of AML cells. The results found that mdr1 mRNA and Pgp did not expressed in HL-60 cells, but significantly overexpressed in HL-60/VCR cells. Except 14-3-3?, the expression of other subtypes of 14-3-3 was higher in HL-60/VCR cells than that in HL-60 cells, especially 14-3-3?. The higher expression of 14-3-3?, BCL-2, MCL-1 protein was observed in HL-60/VCR cells than that in HL-60 cells. These results were same results from gene chip. It was also noticed that 14-3-3? was located in the cytoplasma and nuclei of AML cell lines, especially over-expressed in HL-60/VCR cells. Furthermore, suppression of 14-3-3? by RNA interference resulted in inhibition of the proliferation of AML cells with decreased protein expression of BCL-2 and MCL-1, especially in HL-60/VCR cells. It is concluded that 14-3-3? plays an important role in proliferation of AML cells and associates with BCL-2 and MCL-1 expression. These results suggested that development of therapy targeting 14-3-3? may provide novel, effective strategies for refractory and relapsed AML.
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Xeroderma pigmentosum complementation group f polymorphisms influence risk of glioma.
Asian Pac. J. Cancer Prev.
PUBLISHED: 09-03-2013
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We conducted an exploratory investigation of whether variation in six common SNPs of xeroderma pigmentosum complementation group F (XPF) is associated with risk of glioma in a Chinese population. Six single nucleotide polymorphisms (SNPs) were genotyped in 207 glioma cases and 236 cancer-free controls by a 384-well plate format on the Sequenom MassARRAY platform (Sequenom, San Diego, USA). The rs1800067 G and rs2276466 G allele frequencies were significantly higher in the glioma group than controls. Individuals with the rs1800067 GG genotype were at greater risk of glioma when compared with the A/A genotype in the codominant model, with an OR (95% CI) of 2.63 (1.04-7.25). The rs2276466 polymorphism was significantly associated with moderate increased risk of glioma in codominant and dominant models, with ORs (95% CI) of 1.90 (1.05-3.44) and 1.55 (1.07-2.47), respectively. The combination genotype of rs1800067 G and rs2276466 G alleles was associated with a reduced risk of glioma (OR=0.44, 95% CI=0.19-0.98). These findings indicate that genetic variants of the XPF gene have critical functions in the development of glioma.
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Posaconazole vs. fluconazole as invasive fungal infection prophylaxis in China: a multicenter, randomized, open-label study.
Int J Clin Pharmacol Ther
PUBLISHED: 08-22-2013
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Invasive fungal infection (IFI) is common in neutropenic patients with acute myelogenous leukemia (AML) or myelodysplastic syndrome (MDS). Posaconazole is a broad-spectrum triazole antifungal drug with efficacy in prevention of IFI; however, it has not been previously studied as prophylaxis in a Chinese population.
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[Efficacy comparison between standard and reduced doses of bortezomib combined with adriamycin and dexamethasone in the treatment of patients with multiple myeloma].
Zhonghua Xue Ye Xue Za Zhi
PUBLISHED: 08-03-2013
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To compare the efficacy and safety of standard or reduced doses of bortezomib combined with adriamycin and dexamethasone (PAD) in patients with multiple myeloma (MM).
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An 85-year-old woman with Miller Fisher syndrome.
Pak J Med Sci
PUBLISHED: 05-08-2013
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Miller Fishers syndrome (MFS) commonly presents in the fourth and fifth decades and are rare in people over 70 years. An 85-year-old female with no significant medical history presented with upper extremity anesthesia, ptosis, and unsteady gait. The patient had a history of hypertension and diabetes mellitus. Physical examination showed bilateral total external ophthalmoplegia, areflexia, and cerebellar ataxia. Radiological and laboratory studies were unremarkable. Lumbar puncture showed albuminocytological dissociation. The combined history, physical examination, and lumbar puncture results established a presumptive diagnosis of MFS. Intravenous immunoglobulin was given for 5 days. The patient gradually improved 10 days after the onset of symptoms. Ophthalmoplegia had fully recovered after 6 months. To the best of our knowledge, this case represented the oldest patient with MFS.
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Utilization of gene expression signature for quality control of traditional Chinese medicine formula Si-Wu-Tang.
AAPS J
PUBLISHED: 04-12-2013
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The current study utilized a combined pharmacokinetic and genomic approach to demonstrate the feasibility of a new quality control method by using a panel of special differentially expressed genes (DEGs) as unique fingerprint to serve as marker of in vivo bioactivity for a representative traditional Chinese medicine (TCM) formula, Si-Wu-Tang (SWT). The method involves firstly obtaining possible in vivo active components, i.e., the "absorbable" components from the permeate of the Caco-2 monolayer model to simulate oral administration of two specific SWT products (CU-SWT, J-SWT), their component single herbs (Angelicae, Chuanxiong, Paeoniae, and Rehmanniae), and a standard mixture of active compounds (ferulic acid, ligustilide, senkyunolide A). Then, these respective absorbable components were incubated with MCF-7 cells to determine the gene expression profile using microarray processing/analysis as well as real-time PCR. From the available DEGs identified following the incubation, the magnitude of change in DEGs by real-time PCR was found to be consistent with that by microarray. The designated DEGs from the CU-SWT permeate were found to be distinct from other 19 products. Furthermore, the changes in the DEGs resulting from MCF-7 cells treated by eight replicate extracts of CU-SWT on three separate days were consistent. These results demonstrated sufficient specificity and consistency of the DEG panel which could serve as a unique bioactive "fingerprint" for the designated SWT product. The present method for DEG determination may be applied to other TCM products and with further definitive study can potentially provide a unique method for quality control of TCM in the future.
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IDH1 mutation is associated with improved overall survival in patients with glioblastoma: a meta-analysis.
Tumour Biol.
PUBLISHED: 04-08-2013
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Previous studies proposed that isocitrate dehydrogenase 1 (IDH1) mutation was associated with improved survival in patients with glioblastoma, but those studies reported varying estimates and yielded inconclusive results. The purpose of the present study was to determine the effect of IDH1 mutation on the prognosis of patients with glioblastoma by performing a meta-analysis. Pubmed and Embase databases were searched for eligible studies. Studies reporting overall survival by IDH1 mutation in patients with glioblastoma were considered potentially eligible for the meta-analysis. For the quantitative aggregation of the survival results, the IDH mutation effect was measured by the pooled hazard ratio (HR) with its 95 % confidence interval (95%CI). Nine studies with a total of 1,669 patients with glioblastoma were finally included into this meta-analysis. Overall, the IDH1 mutation was associated with improved survival in patients with glioblastoma (random effects model HR?=?0.45, 95%CI 0.29-0.69, P?
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Oligonorbornenes with hammock-like crown ether pendants as artificial transmembrane ion channel.
Chem Asian J
PUBLISHED: 02-20-2013
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Trimeric oligonorbornenes with hammock-like crown ether pendants 3b and 3c were selectively synthesized by cascade metathetical cyclopolymerization upon treatment with the first generation Grubbs catalyst. These crown-ether-containing oligonorbornenes are impregnated in egg yolk phosphatidylcholine (EYPC) liposome as an artificial ion channel. The efficiency of the sodium ion transport properties has been examined. Oligomer 3c having a polar hydroxy end group exhibits the highest transport efficiency, which is comparable with the best efficiencies reported in literature. The orientation of the crown ether moieties in these oligomers may be critical for the ion transport properties.
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Downregulation of CFTR promotes epithelial-to-mesenchymal transition and is associated with poor prognosis of breast cancer.
Biochim. Biophys. Acta
PUBLISHED: 01-23-2013
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The epithelial-to-mesenchymal transition (EMT), a process involving the breakdown of cell-cell junctions and loss of epithelial polarity, is closely related to cancer development and metastatic progression. While the cystic fibrosis transmembrane conductance regulator (CFTR), a Cl(-) and HCO3(-) conducting anion channel expressed in a wide variety of epithelial cells, has been implicated in the regulation of epithelial polarity, the exact role of CFTR in the pathogenesis of cancer and its possible involvement in EMT process have not been elucidated. Here we report that interfering with CFTR function either by its specific inhibitor or lentiviral miRNA-mediated knockdown mimics TGF-?1-induced EMT and enhances cell migration and invasion in MCF-7. Ectopic overexpression of CFTR in a highly metastatic MDA-231 breast cancer cell line downregulates EMT markers and suppresses cell invasion and migration in vitro, as well as metastasis in vivo. The EMT-suppressing effect of CFTR is found to be associated with its ability to inhibit NF?B targeting urokinase-type plasminogen activator (uPA), known to be involved in the regulation of EMT. More importantly, CFTR expression is found significantly downregulated in primary human breast cancer samples, and is closely associated with poor prognosis in different cohorts of breast cancer patients. Taken together, the present study has demonstrated a previously undefined role of CFTR as an EMT suppressor and its potential as a prognostic indicator in breast cancer.
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Recent adaptive events in human brain revealed by meta-analysis of positively selected genes.
PLoS ONE
PUBLISHED: 01-01-2013
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Analysis of positively-selected genes can help us understand how human evolved, especially the evolution of highly developed cognitive functions. However, previous works have reached conflicting conclusions regarding whether human neuronal genes are over-represented among genes under positive selection.
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Reduced tumor lysis syndrome with low dose chemotherapy for hyperleukocytic acute leukemia prior to induction therapy.
Asian Pac. J. Cancer Prev.
PUBLISHED: 12-01-2011
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Patients with hyperleukocytic acute leukemia (HAL) can succumb to leukostasis. In an attempt to reduce its incidence, 45 patients with newly diagnosed HAL and hyperleukocytosis were administered half the conventional dose of etoposide and cytosine arabinoside (EA: 50 mg/m2 daily each) until WBC counts were significantly reduced and standard induction therapy was initiated. We retrospectively reviewed their outcomes and analyzed potential factors with a logistic regression model. The incidence of early mortality (<30 days) was 4.4% (2/45). Patients who achieved complete remission with induction chemotherapy had significantly lower median WBC counts (26x10(9) L-1) after low dose EA treatment than the no response patients (median WBC: 65x10(9) L-1 (P<0.05). Low dose EA treatment of HAL patients reduced WBC for both lymphoid and myeloid leukemic cells and can be considered for preemptive administration to HAL patients prior to the differential diagnosis of the acute leukemia. This approach warrants further studies as a cytoreduction therapy for HAL.
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Vector-dispersion compensation and pulse pedestal cancellation in a femtosecond nonlinear amplification fiber laser system.
Opt Lett
PUBLISHED: 11-04-2011
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We report on a femtosecond nonlinear amplification fiber laser system using a vector-dispersion compressor, which consists of a transmission grating pair and multipass cell based Gires-Tournois interferometer mirrors. The mirror is designed with nearly zero group-delay dispersion and large negative third-order dispersion. As a result, the third-order dispersion of the compressor can be adjusted independently to compensate the nonlinear phase shift of amplified pulses to reduce the pulse pedestal. With this scheme, the system outputs 44? fs laser pulses with little wing at 26.6? W output average power and 531? nJ pulse energy, corresponding to 10.8 ?MW peak power.
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Different dose combinations of bortezomib and dexamethasone in the treatment of relapsed or refractory myeloma: an open-label, observational, multi-center study in China.
Chin. Med. J.
PUBLISHED: 11-02-2011
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Although previous clinical study revealed that bortezomib combined with dexamethasone had improved the outcomes of relapsed or refractory multiple myeloma (RRMM), the optimal dose combinations of bortezomib and dexamethasone remain unknown. This trial aimed to observe the efficacy and safety of different dose combinations of bortezomib and dexamethasone in the treatment of RRMM patients in China.
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Subcutaneous adipose tissue macrophage infiltration is associated with hepatic and visceral fat deposition, hyperinsulinemia, and stimulation of NF-?B stress pathway.
Diabetes
PUBLISHED: 10-26-2011
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To examine in obese young adults the influence of ethnicity and subcutaneous adipose tissue (SAT) inflammation on hepatic fat fraction (HFF), visceral adipose tissue (VAT) deposition, insulin sensitivity (SI), ?-cell function, and SAT gene expression.
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Overexpression of microRNA-29b induces apoptosis of multiple myeloma cells through down regulating Mcl-1.
Biochem. Biophys. Res. Commun.
PUBLISHED: 09-06-2011
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MicroRNAs (miRNAs) are small, noncoding ribonucleic acids (ncRNAs), which regulate gene expression by targeting mRNAs for translational repression and degradation. Several lines of evidences have indicated that miRNAs act as tumor suppressors and oncogenes. However, the role of miRNAs in pathogenesis of multiple myeloma (MM) remains unclear. In this study, we examined the profile of miRNA expression of primary MM cells, using miRNA microarray and quantitative real-time polymerase chain reaction (qPCR) techniques. These results showed that in the bone marrow specimens analyzed, miRNA-29b was significantly downregulated. Similar results were also observed in human myeloma cell lines (HMCLs). Adenovirus-mediated overexpression of miR-29b induced apoptosis and elevated caspase-3 activation in HMCLs. Using a bioinformatics approach, we found a perfect complementarity between miRNA-29b and the 3UTR of myeloid-cell-leukemia 1(Mcl-1). It is further confirmed that miRNA-29b downregulated the level of Mcl-1 without effect on the mRNA level using both qRT-PCR assays and Western blot analyses. Moreover, we observed that enforced miR-29b expression by using a retarget miRNA-29b expression vector (Ad5F11p-miR-29b) could induce apoptosis and elevate caspase-3 activation in HMCLs. Our results also indicated that miRNA-29b-induced apoptosis acted antagonistically with IL-6 in HMCLs. These findings suggest that miRNA-29b may play an important role in MM as a tumor suppressor.
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Chimeric anti-staphylococcal enterotoxin B antibodies and lovastatin act synergistically to provide in vivo protection against lethal doses of SEB.
PLoS ONE
PUBLISHED: 07-21-2011
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Staphylococcal enterotoxin B (SEB) is one of a family of toxins secreted by Staphylococcus aureus that act as superantigens, activating a large fraction of the T-cell population and inducing production of high levels of inflammatory cytokines that can cause toxic shock syndrome (TSS) and death. Extracellular engagement of the TCR of T-cells and class II MHC of antigen presenting cells by SEB triggers the activation of many intracellular signaling processes. We engineered chimeric antibodies to block the extracellular engagement of cellular receptors by SEB and used a statin to inhibit intracellular signaling. Chimeric human-mouse antibodies directed against different neutralizing epitopes of SEB synergistically inhibited its activation of human T-cells in vitro. In the in vivo model of lethal toxic shock syndrome (TSS) in HLA-DR3 transgenic mice, two of these antibodies conferred significant partial protection when administered individually, but offered complete protection in a synergistic manner when given together. Similarly, in vivo, lovastatin alone conferred only partial protection from TSS similar to single anti-SEB antibodies. However, used in combination with one chimeric neutralizing anti-SEB antibody, lovastatin provided complete protection against lethal TSS in HLA-DR3 transgenic mice. These experiments demonstrate that in vivo protection against lethal doses of SEB can be achieved by a statin of proven clinical safety and chimeric human-mouse antibodies, agents now widely used and known to be of low immunogenicity in human hosts.
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Effect of hydrophilically functionalized carbon nanotubes on the reinforcement of water-borne epoxy resin.
J Nanosci Nanotechnol
PUBLISHED: 07-21-2011
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Multi-walled carbon nanotubes (MWNTs) were modified covalently with hydrophilic polyacrylic acid (PAA) oligomer. Fourier transform infrared (FTIR), thermogravimetric (TG) analysis, transmission electron microscopy (TEM) and scanning electron microscopy (SEM) were used to characterize the modified MWNTs. The solubility of the modified MWNTs in water was determined to be 55.6 mg/mL by UV/vis spectroscopy. They could be dispersed homogeneously in water-borne epoxy resin and improve the mechanical properties of the resin significantly. The reinforcing ability of the modified MWNTs should be contributed by the fine dispersion of the MWNTs in resin matrix and the robust covalent binding formed by the carbon nanotube-bound carboxylic acid and epoxy groups in the resin.
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[Study on status of needle-stick and other sharps injuries among healthcare workers in a general hospital ].
Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi
PUBLISHED: 07-07-2011
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To understand the prevalence of hospital healthcare workers (HCWs) with needle-stick and other sharps injuries, and to provide the basic data for intervention study.
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The effect of hydrophilic chain length and iRGD on drug delivery from poly(?-caprolactone)-poly(N-vinylpyrrolidone) nanoparticles.
Biomaterials
PUBLISHED: 07-06-2011
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Poly(?-caprolactone)-b-Poly(N-vinylpyrrolidone) (PCL-b-PVP) copolymers with different PVP block length were synthesized by xanthate-mediated reverse addition fragment transfer polymerization (RAFT) and the xanthate chain transfer agent on chain end was readily translated to hydroxy or aldehyde for conjugating various functional moieties, such as fluorescent dye, biotin hydrazine and tumor homing peptide iRGD. Thus, PCL-PVP nanoparticles were prepared by these functionalized PCL-b-PVP copolymers. Furthermore, paclitaxel-loaded PCL-PVP nanoparticles with satisfactory drug loading content (15%) and encapsulation efficiency (>90%) were obtained and used in vitro and in vivo antitumor examination. It was demonstrated that the length of PVP block had a significant influence on cytotoxicity, anti-BSA adsorption, circulation time, stealth behavior, biodistribution and antitumor activity for the nanoparticles. iRGD on PCL-PVP nanoparticle surface facilitated the nanoparticles to accumulate in tumor site and enhanced their penetration in tumor tissues, both of which improved the efficacy of paclitaxel-loaded nanoparticles in impeding tumor growth and prolonging the life time of H22 tumor-bearing mice.
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KOBAS 2.0: a web server for annotation and identification of enriched pathways and diseases.
Nucleic Acids Res.
PUBLISHED: 07-01-2011
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High-throughput experimental technologies often identify dozens to hundreds of genes related to, or changed in, a biological or pathological process. From these genes one wants to identify biological pathways that may be involved and diseases that may be implicated. Here, we report a web server, KOBAS 2.0, which annotates an input set of genes with putative pathways and disease relationships based on mapping to genes with known annotations. It allows for both ID mapping and cross-species sequence similarity mapping. It then performs statistical tests to identify statistically significantly enriched pathways and diseases. KOBAS 2.0 incorporates knowledge across 1327 species from 5 pathway databases (KEGG PATHWAY, PID, BioCyc, Reactome and Panther) and 5 human disease databases (OMIM, KEGG DISEASE, FunDO, GAD and NHGRI GWAS Catalog). KOBAS 2.0 can be accessed at http://kobas.cbi.pku.edu.cn.
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Association between HLA-DRB1 and myasthenia gravis in a northern Han Chinese population.
J Clin Neurosci
PUBLISHED: 04-11-2011
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The cause of myasthenia gravis (MG) is unknown, but it is widely believed to be an autoimmune disease occurring in genetically susceptible individuals. The human leukocyte antigen (HLA) region is considered to be the most important genetic region for MG susceptibility genes. To investigate the association between HLA-DRB1 and myasthenia gravis (MG) in a northern Han Chinese population, a polymerase chain reaction with sequence-specific oligonucleotide probe hybridization method was used to determine the HLA-DRB1 genotypes of 91 patients with MG and 171 healthy individuals. We found that the HLA-DRB1(*)09 allele was significantly more prevalent among patients with MG than among healthy controls, especially those who experienced early onset of the disease (?40 years), those who were seronegative for acetylcholine receptor antibody, and those with ocular MG. The prevalence of the HLA-DRB1(*)08 allele was significantly lower among patients with MG than among controls. These results indicate that HLA-DRB1(*)09 might be positively associated and DRB1(*)08 negatively associated with MG in the northern Han Chinese population.
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[The therapeutic effects of highly active anti-retroviral therapy in 74 treatment-naive patients with AIDS in China].
Zhonghua Nei Ke Za Zhi
PUBLISHED: 03-23-2011
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To evaluate the therapeutic effect of highly active anti-retroviral therapy (HAART) in treatment-naïve Chinese patients with AIDS, to provide evidences for standardizing HAART.
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Compressed learning and its applications to subcellular localization.
Protein Pept. Lett.
PUBLISHED: 03-21-2011
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One of the main challenges faced by biological applications is to predict protein subcellular localization in automatic fashion accurately. To achieve this in these applications, a wide variety of machine learning methods have been proposed in recent years. Most of them focus on finding the optimal classification scheme and less of them take the simplifying the complexity of biological systems into account. Traditionally, such bio-data are analyzed by first performing a feature selection before classification. Motivated by CS (Compressed Sensing) theory, we propose the methodology which performs compressed learning with a sparseness criterion such that feature selection and dimension reduction are merged into one analysis. The proposed methodology decreases the complexity of biological system, while increases protein subcellular localization accuracy. Experimental results are quite encouraging, indicating that the aforementioned sparse methods are quite promising in dealing with complicated biological problems, such as predicting the subcellular localization of Gram-negative bacterial proteins.
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High pulse energy mode-locked multicore photonic crystal fiber laser.
Opt Lett
PUBLISHED: 03-16-2011
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A high pulse energy passively mode-locked fiber laser operating in the all-normal dispersion regime is demonstrated. The gain material is an Yb-doped multicore photonic crystal fiber with 18 cores in array-type geometry. Robust and self-starting mode locking is achieved using a fast semiconductor saturable absorber mirror. The laser generates 180?nJ chirped pulses at a 14.48?MHz repetition rate for an average power of 2.6?W. The 1.15?ps output pulses are compressed to 690?fs outside the cavity.
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Quantum codes give counterexamples to the unique preimage conjecture of the N-representability problem.
Phys. Rev. Lett.
PUBLISHED: 03-14-2011
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It is well known that the ground state energy of many-particle Hamiltonians involving only 2-body interactions can be obtained using constrained optimizations over density matrices which arise from reducing an N-particle state. While determining which 2-particle density matrices are "N-representable" is a computationally hard problem, all known extreme N-representable 2-particle reduced density matrices arise from a unique N-particle preimage, satisfying a conjecture established in 1972. We present explicit counterexamples to this conjecture through giving Hamiltonians with 2-body interactions which have degenerate ground states that cannot be distinguished by any 2-body operator. We relate the existence of such counterexamples to quantum error correction codes and topologically ordered spin systems.
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Cloning, expression, and characterization of serine protease from thermophilic fungus Thermoascus aurantiacus var. levisporus.
J. Microbiol.
PUBLISHED: 03-03-2011
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The serine protease gene from a thermophilic fungus Thermoascus aurantiacus var. levisporus, was cloned, sequenced, and expressed in Pichia pastoris and the recombinant protein was characterized. The full-length cDNA of 2,592 bp contains an ORF of 1,482 bp encoding 494 amino acids. Sequence analysis of the deduced amino acid sequence revealed high homology with subtilisin serine proteases. The putative enzyme contained catalytic domain with active sites formed by three residues of Aspl83, His215, and Ser384. The molecular mass of the recombinant enzyme was estimated to be 59.1 kDa after overexpression in P. pastoris. The activity of recombinant protein was 115.58 U/mg. The protease exhibited its maximal activity at 50°C and pH 8.0 and kept thermostable at 60°C, and retained 60% activity after 60 min at 70° C. The protease activity was found to be inhibited by PMSF, but not by DTT or EDTA. The enzyme has broad substrate specificity such as gelatin, casein and pure milk, and exhibiting highest activity towards casein.
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Tumor progression locus 2 (TPL2) regulates obesity-associated inflammation and insulin resistance.
Diabetes
PUBLISHED: 02-23-2011
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Obesity-associated low-grade systemic inflammation resulting from increased adipose mass is strongly related to the development of insulin resistance and type 2 diabetes as well as other metabolic complications. Recent studies have demonstrated that the obese metabolic state can be improved by ablating certain inflammatory signaling pathways. Tumor progression locus 2 (TPL2), a kinase that integrates signals from Toll receptors, cytokine receptors, and inhibitor of ?-B kinase-? is an important regulator of inflammatory pathways. We used TPL2 knockout (KO) mice to investigate the role of TPL2 in mediating obesity-associated inflammation and insulin resistance.
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[Effect of rapamycin on proliferation of acute myeloid leukemia cell lines HL-60 and HL-60/VCR].
Zhongguo Shi Yan Xue Ye Xue Za Zhi
PUBLISHED: 12-24-2010
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In order to investigate the effect of rapamycin on the proliferation of human acute myeloid leukemia (AML) cells, the sensitive cells HL-60 and multidrug-resistant HL-60/VCR cells were chosen as research objects. The proliferation of cells was detected by growth curve method. The flow cytometer was used to analyze cell cycle. The expression of P-glycoprotein (Pgp) was determined by Western blot. The results demonstrated that there was a significant difference of cell growth inhibition rate between control group and rapamycin group (p < 0.05). The cell growth inhibition rate was dose- and time- dependent (p < 0.05). Flow cytometry detection showed that the cell percentage of G(1) phase in rapamycin group was higher than that in group without rapamycin, and that of S phase was lower. The cell growth inhibition rate in 50 nmol/L and 100 nmol/L rapamycin plus daunorubicin (DNR) group was more than that in DNR alone group (p < 0.05), especially when DNR was added at 24 hours interval after RAP. The expression of Pgp of HL-60/VCR cells was inhibited by rapamycin. It is concluded that the rapamycin can inhibit the proliferation of sensitive HL-60 and multidrug resistant HL-60/VCR cells. It can also increase sensitivity of HL-60 and HL-60/VCR cells to DNR, which provides new strategy for the therapy of refractory AML.
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[Effects of xbp-1 gene silencing on bortezomib-induced apoptosis in human multiple myeloma cells].
Zhongguo Shi Yan Xue Ye Xue Za Zhi
PUBLISHED: 12-07-2010
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This study was purposed to investigate the effect of xbp-1 gene silencing on bortezomib-induced apoptosis in multiple myeloma cell line NCI-H929 (H929). After xbp-1 gene expression was interfered by small hairpin RNA, the cell apoptosis was assayed by flow cytometry with Annexin V-FITC/PI staining, and the expression level of XBP-1 protein was detected by Western blot. The results showed that XBP-1 protein level of H929 cells was inhibited effectively by the PLL3.7 lentiviral vector mediated expression xbp-1 shRNA. The apoptosis rate was significantly higher in xbp-1 shRNA-expressing cells than in untreated control group [(10.13±0.61)% vs (2.5±0.2)%, p<0.05]. After treatment with bortezomib, the apoptosis rate of XBP-1 protein functionally deficient H929 cells was significantly higher than those in vector control group [(45.07±1)% vs (19.53±0.8)%, p<0.05]. It is concluded that xbp-1 gene silencing can significantly enhance the pro-apoptotic activity of bortezomib in multiple myeloma cells.
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[An analysis of opportunistic infection in 762 inpatients with human immunodeficiency virus infection in Guangdong areas].
Zhonghua Nei Ke Za Zhi
PUBLISHED: 10-29-2010
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To analyze the characteristics of opportunistic infection (OI) in patients with HIV/AIDS in Guangdong and the relationship between OI and the change in blood CD4+ T lymphocyte count (CD4+).
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Aldolase A-HBsAg interaction and its effect on ultraviolet radiation induced apoptosis in 293FT cells.
J. Gastroenterol. Hepatol.
PUBLISHED: 10-01-2010
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Hepatitis B virus (HBV) infection poses great challenges to humans, claiming one million lives annually worldwide. Solid data have related HBV to hepatocellular carcinoma.
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A host defense mechanism involving CFTR-mediated bicarbonate secretion in bacterial prostatitis.
PLoS ONE
PUBLISHED: 09-02-2010
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Prostatitis is associated with a characteristic increase in prostatic fluid pH; however, the underlying mechanism and its physiological significance have not been elucidated.
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[PI3-kinase mediates thrombin-induced platelet aggregation through mDia1 pathway.].
Zhonghua Xue Ye Xue Za Zhi
PUBLISHED: 06-01-2010
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To investigate the expression of mDia1 (mammalian diaphanous 1)in platelet and the role of mDia1 or phosphatidylinositol 3-kinase (PI3K) in the process of thrombin-induced platelet aggregation.
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[Clinical analysis on 28 patients with hemophagocytic lymphohistiocytosis syndrome].
Zhongguo Shi Yan Xue Ye Xue Za Zhi
PUBLISHED: 04-27-2010
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In order to profoundly understand the clinical and laboratorial characteristics and inducing factors of hemophagocytic lymphohistiocytosis syndrome (HLH), 28 HLH patients received from 2004 to 2009 years in our hospital were analyzed retrospectively. The results indicated that all of the patients had a history with prolonged fever (more than 1 week), pancytopenia, hepatosplenomegaly, elevated ferritin level, hypofibrinogen, and hemophagocytosis in bone marrow. HLH was the first characteristic sign of malignant lymphoma in 9 patients; 1 patient had a clinical manifestation similar to fulminant hepatic failure; severe psycho-abnormality occurred in 1 HLH patient and pronounced hemophagocytosis were detected in his cerebrospinal fluid; 1 patient was eventually diagnosed as having HLH by the findings in a lymph node biopsy showing obvious hemophagocytosis. Additionally, the analysis of underlying factors in 28 patients with HLH indicated 11 patients with EB virus-associated HLH, 11 with lymphoma-associated HLH, 2 with Leishmania-associated HLH, and 3 with autoimmune disease-associated HLH. It is concluded that HLH disease is characterised with high heterogenicity in both clinical features and inducing factors; in addition, the patients from a pasturing area should be paid attention to parasite infection such as leishmania.
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Potent neutralization of staphylococcal enterotoxin B by synergistic action of chimeric antibodies.
Infect. Immun.
PUBLISHED: 03-22-2010
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Staphylococcal enterotoxin B (SEB), a shock-inducing exotoxin synthesized by Staphylococcus aureus, is an important cause of food poisoning and is a class B bioterrorism agent. SEB mediates antigen-independent activation of a major subset of the T-cell population by cross-linking T-cell receptors (TCRs) with class II major histocompatibility complex (MHC-II) molecules of antigen-presenting cells, resulting in the induction of antigen independent proliferation and cytokine secretion by a significant fraction of the T-cell population. Neutralizing antibodies inhibit SEB-mediated T-cell activation by blocking the toxins interaction with the TCR or MHC-II and provide protection against the debilitating effects of this superantigen. We derived and searched a set of monoclonal mouse anti-SEB antibodies to identify neutralizing anti-SEB antibodies that bind to different sites on the toxin. A pair of non-cross-reactive, neutralizing anti-SEB monoclonal antibodies (MAbs) was found, and a combination of these antibodies inhibited SEB-induced T-cell proliferation in a synergistic rather than merely additive manner. In order to engineer antibodies more suitable than mouse MAbs for use in humans, the genes encoding the VL and VH gene segments of a synergistically acting pair of mouse MAbs were grafted, respectively, onto genes encoding the constant regions of human Igkappa and human IgG1, transfected into mammalian cells, and used to generate chimeric versions of these antibodies that had affinity and neutralization profiles essentially identical to their mouse counterparts. When tested in cultures of human peripheral blood mononuclear cells or splenocytes derived from HLA-DR3 transgenic mice, the chimeric human-mouse antibodies synergistically neutralized SEB-induced T-cell activation and cytokine production.
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Quantum state reduction for universal measurement based computation.
Phys. Rev. Lett.
PUBLISHED: 03-08-2010
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Measurement based quantum computation, which requires only single particle measurements on a universal resource state to achieve the full power of quantum computing, has been recognized as one of the most promising models for the physical realization of quantum computers. Despite considerable progress in the past decade, it remains a great challenge to search for new universal resource states with naturally occurring Hamiltonians and to better understand the entanglement structure of these kinds of states. Here we show that most of the resource states currently known can be reduced to the cluster state, the first known universal resource state, via adaptive local measurements at a constant cost. This new quantum state reduction scheme provides simpler proofs of universality of resource states and opens up plenty of space to the search of new resource states.
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Possible mechanism of benign prostatic hyperplasia induced by androgen-estrogen ratios in castrated rats.
Indian J Pharmacol
PUBLISHED: 02-01-2010
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To explore the role of androgen-estrogen balance in benign prostatic hyperplasia (BPH) induced by varying doses of estradiol/testosterone propionate (E(2)/TP) in castrated rats.
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[PI3-kinase mediates activity of RhoA and interaction of RhoA with mDia1 in thrombin-induced platelet aggregation].
Zhongguo Shi Yan Xue Ye Xue Za Zhi
PUBLISHED: 12-25-2009
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The aim of this study was to investigate the role of RhoA/mDia1 pathway in the process of thrombin-induced platelet aggregation and regulatory effect of PI3K inhibitor on this process. The human platelets were isolated from peripheral blood, the activation of RhoA, Rac1 and Cdc42 in the platelet aggregation was detected by GST pull-down assay and immune co-precipitation, the interaction of RhoA, Rac1 and Cdc42 with mDia1 and the formation of complex in the process of platelet aggregation were determined by immune coprecipitation, and the effect of PI3K inhibitor (wortmannin) on above-mentioned process was assayed. The results showed that thrombin elevated the activity of RhoA and the binding capability of RhoA with mDia1 during thrombin-induced platelet aggregation and spreading on Fg coated coverslips. Wortmannin inhibited the rising of RhoA activity and the binding level of RhoA with mDia1 induced by thrombin. Thrombin elevated the activity of Rac1 and Cdc42 during thrombin-induced platelet aggregation, but could not induce binding of Rac1 or Cdc42 with mDia1. Wortmannin could not inhibit the rising of Rac1 and Cdc42 activity induced by thrombin. It is concluded that the PI3-kinase regulates the thrombin-induced actin cytoskeleton reconstitution in platelets by RhoA-mDia1 pathway.
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[Apoptosis of multiple myeloma cells induced by gossypol acetic acid in vitro and its mechanism].
Zhongguo Shi Yan Xue Ye Xue Za Zhi
PUBLISHED: 12-25-2009
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This study was aimed to investigate the apoptosis effect of gossypol acetic acid on classic human multiple myeloma RPMI8226 cell line in vitro and its mechanism. The inhibitory effect on proliferation of RPMI8226 cells was evaluated by means of MTT assay. Cytotoxic effect and apoptosis was identified and analyzed with the aid of transmission electron microscopy, mitochondria membrane potential (MMP) and DNA gel electrophoresis. Meanwhile, Western-blot assay was used to detect the changes of several key cell apoptosis regulatory proteins such as BAX, caspase-3 and caspase-8 in these cells before and after treatment. The results showed that low concentrations of gossypol acetic acid (> 16 micromol/L) could suppress the proliferation and induce the apoptosis in RPMI8226 cells effectively. At the same time, gossypol acetic acid could also down-regulate the mitochondrial membrane potential, up-regulate the expression of the apoptosis-related protein such as BAX and caspase-3. It is concluded that the gossypol acetic acid can selectively induce proliferation inhibition and apoptosis of multiple myeloma RPMI8226 cells with a smaller dose.
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[Changes and mechanism of apoptosis-related gene expression in T lymphocytic leukemia JM cells induced with matrine].
Zhongguo Shi Yan Xue Ye Xue Za Zhi
PUBLISHED: 10-21-2009
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This study was purposed to investigate the changes of apoptosis-related gene expression in T lymphocytic leukemia JM cells induced with matrine, and its possible mechanism. JM cells was induced with 0.4 mg/ml matrine for 4 days, the total RNA was extracted from JM cells before and after matrine induction, the differential expression of apoptosis-related genes were screened with cDNA Expression Array Kit, the expression change of a part of gene was checked by Western blot. The results indicated that after induction of JM cells with matrine, differential expression of 31 genes were found by gene chip hybridization, the expression of caspase 8 was up-regulated more than 5 times. Western blot analysis showed that the up-regulation of caspase 8 gene expression positively correlated with induction time. It is concluded that differential expressions of many apoptosis-related genes in JM cells can be induced by matrine, in which gene expression of caspase 8 is up-regulated notably.
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[Association of glucocorticoid receptor gene polymorphism with myasthenia gravis].
Zhonghua Yi Xue Za Zhi
PUBLISHED: 09-30-2009
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To investigate the association of two glucocorticoid receptor (GR) polymorphisms (BclI, ER22/23EK) with Myasthenia Gravis (MG).
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[PAD regimen for relapsed or refractory patients with multiple myeloma].
Zhonghua Xue Ye Xue Za Zhi
PUBLISHED: 09-08-2009
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To investigate the efficacy and safety of PAD [bortezomib (PS-341), doxorubicin and dexamethasone] regimen for relapsed or refractory multiple myeloma (MM).
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The cystic fibrosis transmembrane conductance regulator in reproductive health and disease.
J. Physiol. (Lond.)
PUBLISHED: 09-04-2009
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The cystic fibrosis transmembrane conductance regulator (CFTR) is an anion channel regulated by cAMP-dependent phosphorylation, which is expressed in epithelial cells of a wide variety of tissues including the reproductive tracts. Mutations in the gene encoding CFTR cause cystic fibrosis, a common genetic disease in Caucasian populations with a multitude of clinical manifestations including infertility/subfertility in both sexes. However, the physiological role of CFTR in reproduction and its involvement in the pathogenesis of reproductive diseases remain largely unknown. This review discusses the role of CFTR in regulating fluid volume and bicarbonate secretion in the reproductive tracts and their importance in various reproductive events. We also discuss the contribution of CFTR dysfunction to a number of pathological conditions. The evidence presented is consistent with an important role of CFTR in reproductive health and disease, suggesting that CFTR might be a potential target for the diagnosis and treatment of reproductive diseases including infertility.
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[Effects of sustained-release alpha-lipoic acid tablet on blood lipid, blood sugar and insulin in hyperlipidemic New Zealand rabbits].
Nan Fang Yi Ke Da Xue Xue Bao
PUBLISHED: 05-01-2009
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To evaluate the effect of sustained-release alpha-lipoic acid tablets (SRLA) on blood lipid, glucose and insulin levels in hyperlipidemic New Zealand rabbits.
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[Biodegradation of di-n-butyl phthalate by Pseudomonas aeruginosa].
Huan Jing Ke Xue
PUBLISHED: 05-01-2009
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A new small capacity-wide extraction method was proposed for detection of its biodegradation in water. Results showed that the halflife (t1/2) of di-n-butyl phthalate (DBP) biodegradation was 3.60 day when the concentration of DBP was 400 mg/L and the biomass concentration was 2 g/L. The biodegradation process conformed to the first-order kinetic model. Moreover, the whole degradation process could be divided into several steps: adsorption, desorption and degradation. Two metabolites of DBP degradation were identified as mono-butyl phthalate and phthalic acid by gas chromatography-mass spectrometry, which confirmed the dioxygenate process during the hydrolysis of DBP.
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[Bcl-6 expression in K562 cells and its role in mechanism underlying induced differentiation into various myelocytic lineages].
Zhongguo Shi Yan Xue Ye Xue Za Zhi
PUBLISHED: 04-22-2009
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This study was purposed to investigate the changes of bcl-6 expression in K562 cells and the mechanism inducing differentiation into different myelocyte lineages. Models of K562 cells inducing differentiation to lineages of megakaryocyte, erythrocyte and macrophagocyte were established with inducers TPA (tetradecanoylphorbol 13-acetate), Hu (hydroxyurea) and HMBA (hexamethylene bisacetamide) respectively. Western blot assay was applied to detect the expression of bcl-6 in K562 cells before and after the induction. Meanwhile, PCR, cloning and direct DNA sequencing were used to identify mutations in the 5 regulatory region of bcl-6 in K562 cells before and after induction with TPA. The results indicated that up-regulation of bcl-6 expression was found only in K562 cells being induced differentiating into megakaryocyte-lineage, while mutation of 5 regulatory region of bcl-6 gene was not found. It is concluded that expression of bcl-6 increases only when K562 cells differentiate into megakaryocyte lineage and bcl-6 expression may play an important role in K562 cells induced differentiation into megakaryocyte lineage. The up-regulation of bcl-6 expression may not be related with the mutation of 5 regulatory regions of the gene.
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Interacting with HBsAg compromises resistance of jumping translocation breakpoint protein to ultraviolet radiation-induced apoptosis in 293FT cells.
Cancer Lett.
PUBLISHED: 04-11-2009
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Jumping translocation breakpoint protein (JTB) is suppressed in many cancers, implying it plays a role in the neoplastic transformation of cells. In order to explore the role of JTB in the carcinogenesis of liver, we used mammalian two-hybrid, co-immunoprecipitation, GST pull-down and laser scanning confocal to verify the interaction between HBs and JTB. According to the results, HBs interacts with JTB. In addition, we further determined that S region within HBs is sufficient for binding JTB. Overexpression of JTB conferred resistance to apoptosis induced by ultraviolet radiation, whereas this effect was compromised by the co-overexpression of HBs.
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What is Visualize?

JoVE Visualize is a tool created to match the last 5 years of PubMed publications to methods in JoVE's video library.

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We use abstracts found on PubMed and match them to JoVE videos to create a list of 10 to 30 related methods videos.

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In developing our video relationships, we compare around 5 million PubMed articles to our library of over 4,500 methods videos. In some cases the language used in the PubMed abstracts makes matching that content to a JoVE video difficult. In other cases, there happens not to be any content in our video library that is relevant to the topic of a given abstract. In these cases, our algorithms are trying their best to display videos with relevant content, which can sometimes result in matched videos with only a slight relation.