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Find video protocols related to scientific articles indexed in Pubmed.
New Mutations in the RAB28 Gene in 2 Spanish Families With Cone-Rod Dystrophy.
JAMA Ophthalmol
PUBLISHED: 10-31-2014
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The families evaluated in this study represent the second report of cone-rod dystrophy (CRD) cases caused by mutations in RAB28, a recently discovered gene associated with CRD.
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Ubiquitin specific protease 22 promotes cell proliferation and tumor growth of epithelial ovarian cancer through synergy with transforming growth factor ?1.
Oncol. Rep.
PUBLISHED: 08-21-2014
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Ubiquitin specific protease 22 (USP22) is an oncogene that is upregulated in many cancer types, and aberrant expression of USP22 correlates with clinical outcome. However, its potential functional impact in epithelial ovarian cancer (EOC) has not been determined. Here, we report that USP22 was upregulated in EOC specimens and EOC cell lines with important functional consequences. A high level of USP22 in EOC tissues was associated with advanced clinical FIGO stage, lymph node metastasis and worse prognosis. Patients with higher USP22 expression had shorter relapse-free and overall survival. Depletion of USP22 suppressed cell proliferation in vitro and tumor growth in vivo. We found that inhibition of USP22 suppressed cell proliferation by inducing G1 phase cell cycle arrest through synergy with oncogenic transforming growth factor-?1 (TGFB1). Our results indicate that USP22 functions as an oncogene in EOC, and thus USP22 may serve as a potential therapeutic target for individualized EOC treatment.
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Metformin combined with p38 MAPK inhibitor improves cisplatin sensitivity in cisplatin?resistant ovarian cancer.
Mol Med Rep
PUBLISHED: 08-14-2014
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The aim of the present study was to determine the effects of metformin, combined with a p38 mitogen?activated protein kinase (MAPK) inhibitor, on the sensitivity of cisplatin?resistant ovarian cancer to cisplatin. The expression and distribution of phosphorylated p38 MAPK (P?p38 MAPK) was confirmed in drug?resistant and primary ovarian cancer tissues by immunohistochemistry and western blotting. A bromodeoxyuridine ELISA kit was used to analyze the effects of metformin, SB203580, a p38 MAPK inhibitor, and metformin combined with SB203580, on the cell proliferation of SKOV3/DDP cisplatin?resistant ovarian cancer cells. The protein expression of P?p38 MAPK was significantly higher in cisplatin?resistant ovarian cancer, as compared with the primary ovarian cancer tissues. Metformin combined with SB203580 significantly enhanced the sensitivity of SKOV3/DDP cells to cisplatin. In conclusion, the p38 MAPK signaling pathway may be associated with cisplatin?resistant ovarian cancer. Metformin, combined with the p38 MAPK inhibitor, significantly increased the sensitivity of SKOV3/DDP cells to cisplatin treatment.
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Investigation of the adhesive bonding technology for the insulator structure of EAST neutral beam injector.
Rev Sci Instrum
PUBLISHED: 08-03-2014
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A key issue on the development of EAST ion source was the junction design of insulator structure, which consists of three insulators and four supporting flanges of electrode grid. Because the ion source is installed on the vertical plane, the insulator structure has to withstand large bending and shear stress due to the gravity of whole ion source. Through a mechanical analysis, it was calculated that the maximum bending normal stress was 0.34 MPa and shear stress was 0.23 MPa on the insulator structure. Due to the advantages of simplicity and high strength, the adhesive bonding technology was applied to the junction of insulator structure. A tensile testing campaign of different junction designs between insulator and supporting flange was performed, and a junction design of stainless steel and fiber enhanced epoxy resin with epoxy adhesive was determined. The insulator structure based on the determined design can satisfy both the requirements of high-voltage holding and mechanical strength.
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Analysis of the ABCA4 genomic locus in Stargardt disease.
Hum. Mol. Genet.
PUBLISHED: 07-31-2014
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Autosomal recessive Stargardt disease (STGD1, MIM 248200) is caused by mutations in the ABCA4 gene. Complete sequencing of ABCA4 in STGD patients identifies compound heterozygous or homozygous disease-associated alleles in 65-70% of patients and only one mutation in 15-20% of patients. This study was designed to find the missing disease-causing ABCA4 variation by a combination of next-generation sequencing (NGS), array-Comparative Genome Hybridization (aCGH) screening, familial segregation and in silico analyses. The entire 140 kb ABCA4 genomic locus was sequenced in 114 STGD patients with one known ABCA4 exonic mutation revealing, on average, 200 intronic variants per sample. Filtering of these data resulted in 141 candidates for new mutations. Two variants were detected in four samples, two in three samples, and 20 variants in two samples, the remaining 117 new variants were detected only once. Multimodal analysis suggested 12 new likely pathogenic intronic ABCA4 variants, some of which were specific to (isolated) ethnic groups. No copy number variation (large deletions and insertions) was detected in any patient suggesting that it is a very rare event in the ABCA4 locus. Many variants were excluded since they were not conserved in non-human primates, were frequent in African populations and, therefore, represented ancestral, and not disease-associated, variants. The sequence variability in the ABCA4 locus is extensive and the non-coding sequences do not harbor frequent mutations in STGD patients of European-American descent. Defining disease-associated alleles in the ABCA4 locus requires exceptionally well characterized large cohorts and extensive analyses by a combination of various approaches.
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Design, Synthesis, and Biological Activity of Oxime Ether Strobilurin Derivatives Containing Indole Moiety as Novel Fungicide.
Chem Biol Drug Des
PUBLISHED: 06-30-2014
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Twenty-one novel oxime ether strobilurins containing indole moiety, which employed an indole group to stabilize the E-styryl group in Enoxastrobin, were designed and synthesized. The biological assay indicated that most compounds exhibited potent fungicidal activities. The structure-activity relationship study demonstrated that the synthesized methyl 3-methoxypropenoate oxime ethers 7b-e exhibited remarkably high activities among all the synthesized oxime ether compounds 7. Moreover, the fungicidal activities of methyl ?-(methoxyimino)benzeneacetate oxime ethers compounds 7f-i and N-methoxy-carbamic acid methyl esters compounds 7j-m showed significant differences compared to the corresponding products of ammonolysis.
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[Framework analysis method in qualitative research].
Zhongguo Zhong Xi Yi Jie He Za Zhi
PUBLISHED: 06-20-2014
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In recent years a number of qualitative research methods have gained popularity within the health care arena. Despite this popularity, different qualitative analysis methods pose many challenges to most researchers. The present paper responds to the needs expressed by recent Chinese medicine researches. The present paper is mainly focused on the concepts, nature, application of framework analysis, especially on how to use it, in such a way to assist the newcomer of Chinese medicine researchers to engage with the methodology.
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New syndrome with retinitis pigmentosa is caused by nonsense mutations in retinol dehydrogenase RDH11.
Hum. Mol. Genet.
PUBLISHED: 06-10-2014
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Retinitis pigmentosa (RP), a genetically heterogeneous group of retinopathies that occur in both non-syndromic and syndromic forms, is caused by mutations in ?100 genes. Although recent advances in next-generation sequencing have aided in the discovery of novel RP genes, a number of the underlying contributing genes and loci remain to be identified. We investigated three siblings, born to asymptomatic parents of Italian-American descent, who each presented with atypical RP with systemic features, including facial dysmorphologies, psychomotor developmental delays recognized since early childhood, learning disabilities and short stature. RP-associated ophthalmological findings included salt-and-pepper retinopathy, attenuation of the arterioles and generalized rod-cone dysfunction as determined by almost extinguished electroretinogram in 2 of 3 siblings. Atypical for RP features included mottled macula at an early age and peripapillary sparing of the retinal pigment epithelium. Whole-exome sequencing data, queried under a recessive model of inheritance, identified compound heterozygous stop mutations, c.C199T:p.R67* and c.C322T:p.R108*, in the retinol dehydrogenase 11 (RDH11) gene, resulting in a non-functional protein, in all affected children. In summary, deleterious mutations in RDH11, an important enzyme for vision-related and systemic retinoic acid metabolism, cause a new syndrome with RP.
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catena-Poly[[di-chlorido-mercury(II)]-?-1,4-bis-[2-(pyridin-4-yl)ethyn-yl]benzene-?(2) N:N'].
Acta Crystallogr Sect E Struct Rep Online
PUBLISHED: 06-01-2014
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In the polymeric title compound, [HgCl2(C20H12N2)] n , the Hg(II) atom is located on a twofold rotation axis and the benzene ring of the bidentate bridging 1,4-bis-[2-(pyridin-4-yl)ethyn-yl]benzene (L) ligand is located about a twofold rotation axis. The Hg(II) atom is coordinated by two N atoms of two different L ligands and by two chloride ions in a distorted tetra-hedral geometry. The dihedral angle between the coordinating pyridine and the benzene ring is 12.8?(2)°. The result of the bridging is the formation of a zigzag chain running parallel to [102]. The chains pack with no specific inter-molecular inter-actions between them.
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Design, synthesis and structure-activity relationship of novel oxime ether strobilurin derivatives containing substituted benzofurans.
Pest Manag. Sci.
PUBLISHED: 04-02-2014
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Strobilurins are one of the most important classes of agricultural fungicides. To discover new strobilurin analogues with broad spectrum and high activity, a series of novel oxime ether strobilurin derivatives containing substituted benzofurans in the side chain were synthesised and bioassayed.
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Stimulated emission in GaN-based laser diodes far below the threshold region.
Opt Express
PUBLISHED: 03-26-2014
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We identify that the stimulated emission of GaN laser diodes (LDs) emerges far below the traditionally recognized threshold from both optical and electrical experiments. Below the threshold, the linear-polarized stimulated emission has been the dominating part of overall emission and closely related to resonant cavity. Its intensity increases super linearly with current while that of spontaneous emission increases almost linearly. Moreover, the separation of quasi-Fermi levels of electrons and holes across the active region has already exceeded the photon emission energy, namely, realized the population-inversion.
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Urantide improves atherosclerosis by controlling C-reactive protein, monocyte chemotactic protein-1 and transforming growth factor-? expression in rats.
Exp Ther Med
PUBLISHED: 03-18-2014
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The aim of the present study was to investigate the effects of urantide on the expression status of C-reactive protein (CRP) and the inflammatory cytokines monocyte chemotactic protein (MCP)-1 and transforming growth factor (TGF)-? in the aortas of rats with atherosclerosis (AS), and to identify its underlying mechanisms. The effects of urantide in a rat model of AS and in cultured rat vascular smooth muscle cells (VSMCs) were analyzed via hematoxylin and eosin staining, immunohistochemical staining and ELISA. The results in vivo demonstrated that urantide downregulated the expression of inflammatory mediators CRP and MCP-1 and upregulated the expression of TGF-?. The results in vitro indicated that urantide inhibited the proliferation of VSMCs. In addition, urantide reduced the expression of CRP and downregulated the secretion of TGF-? in the culture supernatant. In conclusion, urantide ameliorated the arterial inflammatory damage that was observed in the AS rat model at the cell and tissue levels by controlling the expression of CRP and the inflammatory cytokines MCP-1 and TGF-?. Therefore, urantide may be a potential agent for the complementary treatment of AS.
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GSK3? promotes the differentiation of oligodendrocyte precursor cells via ?-catenin-mediated transcriptional regulation.
Mol. Neurobiol.
PUBLISHED: 03-11-2014
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Oligodendrocytes are generated by the differentiation and maturation of oligodendrocyte precursor cells (OPCs). The failure of OPC differentiation is a major cause of demyelinating diseases; thus, identifying the molecular mechanisms that affect OPC differentiation is critical for understanding the myelination process and repairing after demyelination. Although prevailing evidence shows that OPC differentiation is a highly coordinated process controlled by multiple extrinsic and intrinsic factors, such as growth factors, axon signals, and transcription factors, the intracellular signaling in OPC differentiation is still unclear. Here, we showed that glycogen synthase kinase 3? (GSK3?) is an essential positive modulator of OPC differentiation. Both pharmacologic inhibition and knockdown of GSK3? remarkably suppressed OPC differentiation. Terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling assays and Ki67 staining showed that the effect of GSK3? on OPC differentiation was not via cell death. Conversely, activated GSK3? was sufficient to promote OPC differentiation. Our results also demonstrated that the transcription of myelin genes was regulated by GSK3? inhibition, accompanying accumulated nuclear ?-catenin, and reduced the expression of transcriptional factors that are relevant to the expression of myelin genes. Taken together, our study identified GSK3? as a profound positive regulator of OPC differentiation, suggesting that GSK3? may contribute to the inefficient regeneration of oligodendrocytes and myelin repair after demyelination.
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Whole exome sequencing identifies CRB1 defect in an unusual maculopathy phenotype.
Ophthalmology
PUBLISHED: 03-07-2014
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To report a new phenotype caused by mutations in the CRB1 gene in a family with 2 affected siblings.
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Domino reactions of vinyl malononitriles with 3-phenacylideneoxindoles for efficient synthesis of functionalized spirocyclic oxindoles.
ACS Comb Sci
PUBLISHED: 03-04-2014
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The reactions of vinyl malononitriles with 3-phenacylideneoxindoles in ethanol in the presence of DBU as base resulted in the functionalized spirocyclic oxindoles through the domino Michael addition and intramolecular nucleophilic addition to cyano group. On the other hand, the similar reaction in the presence of piperidine as base afforded the simple Michael addition products in good yields. The stereochemistry of the spirooxindoles was established with (1)H NMR data and single crystal structures.
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Synthesis and fungicidal activities of novel benzothiophene-substituted oxime ether strobilurins.
Bioorg. Med. Chem. Lett.
PUBLISHED: 01-28-2014
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Twenty-one novel benzothiophene-substituted oxime ether strobilurins, which employed a benzothiophene group to stabilise the E-styryl group in Enoxastrobin (an unsaturated oxime strobilurin fungicide developed by Shenyang Research Institute of Chemical Industry, China) were designed and synthesised. The biological assay indicated that most compounds exhibited good or excellent fungicidal activities, especially against Colletotrichum lagenarium and Puccinia sorghi Schw. In addition, methyl 3-methoxypropenoate oxime ethers and N-methoxy-carbamic acid methyl esters exhibited good in vivo fungicidal activities against Erysiphe graminis, Colletotrichum lagenarium and Puccinia sorghi Schw. under the tested concentrations. Notably, (E,E)-methyl 3-methoxy-2-(2-((((6-chloro-1-(1H-benzo[b]thien-2-yl)ethylidene)amino)oxy)methyl)phenyl)propenoate (5E) exhibited more potent in vivo fungicidal activities against nearly all of the tested fungi at a concentration of 0.39 mg/L compared to Enoxastrobin.
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Regulation of insulin-like growth factor signaling by metformin in endometrial cancer cells.
Oncol Lett
PUBLISHED: 01-09-2014
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Obesity, diabetes and insulin resistance are marked risk factors that promote the development of type I endometrial cancer. Previous studies have demonstrated that insulin-like growth factor 1 (IGF-1) and IGF-2 promote cell proliferation in endometrial cancer cells, while metformin reverses this effect and inhibits cell proliferation. However, the effects of metformin on the regulation of the IGF signaling pathway are unclear. The aim of this study was to investigate the regulation of IGF signaling by metformin in endometrial cancer cells, and to determine the effects of metformin combined with IGF-1 receptor (IGF-1R) inhibitor on cell proliferation and apoptosis. Cell proliferation was assessed following exposure of Ishikawa and HEC-1B endometrial cancer cell lines to metformin and/or the IGF-1R inhibitor, PPP. Apoptosis was assessed by TdT-mediated dUTP nick end labeling assay. Metformin was observed to downregulate IGF-1R and upregulate IGF binding protein-1 (IGFBP-1) mRNA and protein expression, while compound C, an adenosine monophosphate protein kinase inhibitor, reversed this effect. Metformin administered with PPP inhibited endometrial cancer cell proliferation to a greater degree than treatment with either agent alone. At high concentrations (1 or 2 mM), metformin induced apoptosis in endometrial cancer cells. Metformin combined with IGF-1R axis inhibitors may act synergistically to kill tumor cells, as metformin was shown to delay and prevent IGF-1R feedback. In conclusion, this study supported the results of animal studies and subclinical studies, demonstrating the feasibility of metformin combined with IGF-1R axis inhibitors in the treatment of endometrial cancer.
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Epigallocatechin-3-gallate inhibits cell growth, induces apoptosis and causes S phase arrest in hepatocellular carcinoma by suppressing the AKT pathway.
Int. J. Oncol.
PUBLISHED: 01-08-2014
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Epigallocatechin-3-gallate (EGCG) has been shown to inhibit the growth and induce apoptosis of certain cancer cells. The aim of this study was to determine the role of EGCG in hepatocellular carcinoma (HCC) and the underlying mechanism(s) thereof. MTT assay was used to determine the cell growth inhibition by EGCG. Apoptosis induced by EGCG was investigated by both AO/EB staining and flow cytometry. The cell cycle distribution was analyzed by flow cytometry. The mRNA levels of the AKT pathway were analyzed by quantitative PCR. The expression of AKT and its phosphorylation at Ser473 were detected by western blotting. The IC50 of EGCG at 48 h for HepG2, SMMC7721 and SK-hep1 cells were 74.7, 59.6 and 61.3 µg/ml, respectively. Significantly higher proportion of SMMC7721 cells entered the S phase upon treatment with EGCG for 48 h compared with control cells. EGCG decreased the mRNA levels of PI3K, AKT and NF-?B. The protein levels of AKT decreased and its phosphorylation at Ser473 was downregulated with EGCG treatment. EGCG inhibited growth by affecting the cell cycle and induced apoptosis in different HCC cells by downregulating PI3K/AKT activity. The results suggest the potential of EGCG as an anticancer agent in the prevention or treatment of HCC.
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A constructed HLA-A2-restricted pMAGE-A1278-286 tetramer detects specific cytotoxic T lymphocytes in tumour tissues in situ.
J. Int. Med. Res.
PUBLISHED: 09-26-2013
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To construct a human leucocyte antigen (HLA)-A2-restricted peptide 278-286 of melanoma-associated antigen family A, 1 (pMAGE-A1278-286) tetramer to analyse the distribution of cytotoxic T lymphocytes (CTLs) in tumour tissue and tumour-adjacent normal tissue.
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Two-stage model of chemically induced hepatocellular carcinoma in mouse.
Oncol. Res.
PUBLISHED: 09-26-2013
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The aim of this study was to develop an efficient and reproducible mouse model for hepatocellular carcinoma (HCC) research and assess the expression of two proto-oncogenes (c-myc and N-ras) and tumor suppressor gene p53 in the carcinogenic process. In this study, we found that diethylnitrosamine initiation with CCl4 and ethanol promotion could induce a short-term, two-stage liver carcinogenesis model in male BALB/c mice, the process of hepatocarcinogenesis including liver damage, liver necrosis/cell death, liver inflammation, liver proliferation, liver hyperplasia, liver steatosis, and liver cirrhosis and hepatocellular nodules, which mimicked the usual sequence of events observed in human HCC. We also identified that the increase in expression of the p53 gene is related to the proliferation of hepatocytes, whereas overexpression of the c-myc and N-ras genes is associated with hepatocarcinogenesis. This animal model may serve as a basis for recapitulating the molecular pathogenesis of HCC seen in humans.
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Perioperative acute myocardial infarction increases mortality following noncardiac surgery.
J. Cardiothorac. Vasc. Anesth.
PUBLISHED: 09-20-2013
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To provide insight into diagnosis, treatment, and prevention of perioperative myocardial infarction (PMI).
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Phosphorescent sensing of Cr3+ with protein-functionalized Mn-doped ZnS quantum dots.
Analyst
PUBLISHED: 09-04-2013
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It was found that the phosphorescence from denatured bovine serum albumin (dBSA)-capped Mn-doped ZnS QDs could be selectively quenched by Cr(3+), and a phosphorescent probe for Cr(3+) was thus developed. Based on phosphorescence decay as well as calculations of the relative energies of QDs and Cr(3+), the mechanism for phosphorescent quenching was preliminarily ascribed to electron transfer from photo-excited Mn-doped ZnS QDs to Cr(3+). Under the optimal conditions, good linear Stern-Volmer quenching was obtained for Cr(3+) in the range of 10 to 300 nM. The limit of detection of this phosphorescence probe (3 nM) was 1 to 2 orders of magnitude lower than those of previously reported nanosensors, owing to the effective elimination of background fluorescence and scattering from the sample matrix. The analytical potential of the proposed probe was evaluated through determination of Cr(3+) in water samples, with spike-recoveries ranging from 95 to 106%.
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Construction of dispirocyclopentanebisoxindoles via self-domino Michael-aldol reactions of 3-phenacylideneoxindoles.
J. Org. Chem.
PUBLISHED: 08-26-2013
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A simple protocol for the construction of novel dispirocyclopentanebisoxindoles is accomplished by the base promoted domino reactions between two molecules of 3-phenacylideneoxindoles with the participation of solvents, alcohol, or other added nucleophiles such as amines or thiophenols. Significantly, this domino reaction results in the complex dispiro compounds with high yields and diastereoselectivity, which would allow construction of dispirocyclopentanebisoxindole with four and five diastereoisomeric centers using simple materials.
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Upregulation of glutamate transporter GLT-1 by mTOR-Akt-NF-?B cascade in astrocytic oxygen-glucose deprivation.
Glia
PUBLISHED: 07-24-2013
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Excessive extracellular glutamate leads to neuronal death in central nervous system. Excitatory glutamate transporter subtype 2 (GLT-1) carries bulk of glutamate reuptake in cerebral ischemia. Although GLT-1 expression fluctuates during the period of ischemia, little is known about its regulatory mechanism. Here we show an up-regulation of GLT-1 via mammalian target of rapamycin (mTOR)-Akt-nuclear factor-?B (NF-?B) signaling cascade in oxygen glucose deprivation (OGD). We found that brief rapamycin treatment significantly increased GLT-1 expression in cultured astrocytes. Rapamycin increased phosphorylation of raptor at Ser792 and decreased phosphorylation of rictor at Thr1135, suggesting that both mTOR complex 1 (mTORC1) and mTOR complex 2 (mTORC2) are involved in GLT-1 expression. This conclusion was further confirmed by raptor and rictor disruption experiments. Akt was activated by mTORC1 inhibition and required for GLT-1 expression because triciribine, a specific inhibitor of Akt, blocked the increase of GLT-1 expression. mTOR-Akt cascade then activated NF-?B and increased ?B-motif-binding phosphoprotein (KBBP) expression and GLT-1 transcription. We next demonstrated that mTOR-Akt-NF-?B cascade was activated in OGD and subsequently caused the upregulation of GLT-1. Supporting evidence included: (1) inhibition of Akt or NF-?B occluded OGD-induced GLT-1 upregulation; (2) Raptor knock-down plus OGD did not add to the increase of GLT-1 expression; (3) Intact mTORC2 was required for GLT-1 enhancement. In summary, our data first showed that mTOR-Akt-NF-?B cascade played critical roles to up-regulate GLT-1 in OGD. This signaling cascade may work to promote glutamate uptake in brain ischemia and neurodegenerative diseases.
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Distinct functions of nuclear distribution proteins LIS1, Ndel1 and NudCL in regulating axonal mitochondrial transport.
Traffic
PUBLISHED: 03-26-2013
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Neurons critically depend on the long-distance transport of mitochondria. Motor proteins kinesin and dynein control anterograde and retrograde mitochondrial transport, respectively in axons. The regulatory molecules that link them to mitochondria need to be better characterized. Nuclear distribution (Nud) family proteins LIS1, Ndel1 and NudCL are critical components of cytoplasmic dynein complex. Roles of these Nud proteins in neuronal mitochondrial transport are unknown. Here we report distinct functions of LIS1, Ndel1 and NudCL on axonal mitochondrial transport in cultured hippocampal neurons. We found that LIS1 interacted with kinsein family protein KIF5b. Depletion of LIS1 enormously suppressed mitochondrial motility in both anterograde and retrograde directions. Inhibition of either Ndel1 or NudCL only partially reduced retrograde mitochondrial motility. However, knocking down both Ndel1 and NudCL almost blocked retrograde mitochondrial transport, suggesting these proteins may work together to regulate retrograde mitochondrial transport through linking dynein-LIS1 complex. Taken together, our results uncover novel roles of LIS1, Ndel1 and NudCL in the transport of mitochondria in axons.
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Management of intractable epistaxis in patients who received radiation therapy for nasopharyngeal carcinoma.
Eur Arch Otorhinolaryngol
PUBLISHED: 03-08-2013
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To report clinical manifestations, bleeding point localization, and outcomes of management in 16 patients with 16 instances of intractable epistaxis after radiation therapy for nasopharyngeal carcinoma. Retrospective chart review of 16 patients with nasopharyngeal carcinoma (mean age 52.06 ± 14.37 years) with 16 instances of intractable epistaxis during the past 5 years, whose diagnosis was confirmed by angiography (n = 10) or MRI/CT imaging studies and clinical manifestations (n = 6). The mean radiation dose to the affected carotid artery was 101.37 ± 34.85 Gy. Bleeding points were detected in the internal carotid artery (n = 8) or external carotid artery (n = 8). Detachable balloons were used in one affected artery for vascular occlusion; six were treated using an absorbable gelatin sponge (n = 4) or microcoils (diameter 1 mm) (n = 2). Endovascular embolization was successful in seven radiation carotid blowout syndromes with cessation of hemorrhage. One patient underwent external carotid artery ligation and one patient recovered without treatment. The clinical follow-up was 3 months. Therapeutic endovascular embolization of intractable epistaxis is both efficient and safe. It should be considered as the primary treatment modality in intractable epistaxis of nasopharyngeal carcinoma.
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A correlation analysis between the expression of pregnancy-associated plasma protein A in basal decidual cells and recurrent spontaneous abortion.
Exp Ther Med
PUBLISHED: 02-26-2013
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The aim of this study was to investigate the correlation between the expression of pregnancy-associated plasma protein A (PAPP-A) in basal decidual cells and recurrent spontaneous abortion (RSA). A total of 39 patients with a history of RSA were enrolled into the RSA group. A further 30 females who had experienced normal pregnancy were enrolled into the control group. The mRNA expression of PAPP-A in basal decidual cells was analyzed using real-time PCR. The distribution and expression of PAPP-A protein levels in basal decidual cells were analyzed by immunohistochemistry. The correlation between PAPP-A protein levels and RSA was analyzed. The levels of PAPP-A mRNA in the RSA group were significantly decreased, compared with the control group (P<0.05). Consistent with the mRNA levels, the protein levels of PAPP-A were also significantly lower in the RSA group compared with the control group (P<0.05). Multivariate logistic analysis indicated that the suppression of PAPP-A was one of the risk factors for RSA. Furthermore, Hosmer-Lemeshow analysis suggested that the expression levels of PAPP-A is an important factor for predicting RSA. In conclusion, the expression levels of the PAPP-A protein were significantly reduced in basal decidual cells of the RSA group compared with the control group. Therefore, PAPP-A is likely to play an important role in RSA.
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Ultra-sensitive graphene-plasmonic hybrid platform for label-free detection.
Adv. Mater. Weinheim
PUBLISHED: 02-06-2013
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A graphene-Au nano-pyramid hybrid system that enables label-free single molecule detection is demonstrated. The bio-compatible graphene-based SERS platform boosts a high density of hot spots with local SERS enhancement factor over 10(10) . We demonstrate that graphene can play a key role in quantitative study of SERS mechanisms, and can also serve as a promising building block in SERS active structures especially for biosensor applications.
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The urotensin II receptor antagonist, urantide, protects against atherosclerosis in rats.
Exp Ther Med
PUBLISHED: 01-23-2013
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The aim of this study was to explore the use of urantide as an antagonist of the urotensin II (UII) receptor, G protein-coupled receptor 14 (GPR14), to protect against atherosclerosis (AS) in rats. The AS rat model was induced by an intraperitoneal injection of vitamin D3 (VD3) into rats fed with a high-fat diet for four weeks. Urantide was then injected into the rats. Immunohistochemical staining, serum biochemical assay, reverse transcription-polymerase chain reaction (RT-PCR) and western blotting were used to investigate the expression of UII and its receptor GPR14 in the AS rat model. Four weeks after induction, pathological changes typical of AS were observed in the AS rat model. In the plaques of the aortic tunica intima and tunica media, expression of UII and GPR14 was observed. The protein and gene expression levels of UII and GPR14 in the model group were significantly increased compared with those in the normal group (P<0.01). Urantide ameliorated the pathological changes of AS in the rat model and reduced the gene and protein expression levels of UII and GPR14 (P<0.05 or P<0.01). UII is associated with AS and the UII receptor GPR14-specific antagonist, urantide, demonstrates the ability to protect against AS. Thus, this study provides new insight and experimental theories for the clinical application of urantide to treat AS.
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Gastrodin attenuation of the inflammatory response in H9c2 cardiomyocytes involves inhibition of NF-?B and MAPKs activation via the phosphatidylinositol 3-kinase signaling.
Biochem. Pharmacol.
PUBLISHED: 01-22-2013
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The phenolic glucoside gastrodin, a main constituent of a Chinese traditional herbal medicine, has been known to display several biological and pharmacological properties. However, the role and precise molecular mechanisms explaining how gastrodin suppresses the inflammatory response in septic cardiac dysfunction are unknown. To study this, rat H9c2 cardiomyocytes were treated with gastrodin and/or lipopolysaccharide (LPS). Our results showed that gastrodin treatment strongly suppressed nuclear factor-?B (NF-?B) and mitogen-activated protein kinases (MAPKs) family activation and upregulation of the expression of inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), tumor necrosis factor-? (TNF-?), and interleukin-6 (IL-6) in LPS-stimulated H9c2 cardiomyocytes. Simultaneously, gastrodin obviously upregulated the phosphatidylinositol 3-kinase (PI3-K)/Akt signaling in a dose-dependent manner. However, wortmannin, a specific PI3-K inhibitor, blocked the inhibitory effects of gastrodin on LPS-stimulated H9c2 cardiomyocytes. Furthermore, PI3-K/Akt inhibition partially abolished the inhibitory effects of gastrodin on the phosphorylation of inhibitor ?B-? (I?B-?), extracellular signal-regulated kinase 1/2 (ERK1/2), c-Jun N-terminal protein kinase (JNK), and p38 mitogen-activated protein kinase (p38 MAPK), and activity of NF-?B. Here we report activation of the PI3-K/Akt signaling by gastrodin and that inhibition of this pathway reverses the inhibitory effects of gastrodin on NF-?B and MAPKs activation in H9c2 cardiomyocytes.
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C-Terminal Domain of ICA69 Interacts with PICK1 and Acts on Trafficking of PICK1-PKC? Complex and Cerebellar Plasticity.
PLoS ONE
PUBLISHED: 01-01-2013
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PICK1 (protein interacting with C-kinase 1) is a PKC (protein kinase C)-binding protein, which is essential for synaptic plasticity. The trafficking of PKC?-PICK1 complex to plasma membrane is critical for the internalization of GluR2 and induction of long-term depression. ICA69 (islet cell autoantigen 69 kDa) is identified as a major binding partner of PICK1. While heteromeric BAR domain complex is suggested to underlie the interaction between PICK1 and ICA69, the role of C-terminal domain of ICA69 (ICAC) in PICK1-ICA69 complex is unknown.
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[Silkworm excrement organic fertilizer: its nutrient properties and application effect].
Ying Yong Sheng Tai Xue Bao
PUBLISHED: 10-20-2011
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In this paper, silkworm excrement was harmless-treated via controlled fermentation to prepare silkworm excrement organic fertilizer (SEOF). The nutrient properties of the SEOF were determined, and a pot experiment was conducted to examine the application effect of the fertilizer. After fermentation, the total N, P, and K contents in the SEOF had a significant increase, being 58.0%, 84.4% , and 29.7% higher than those in the raw material, respectively. The addition of microbial inoculants shortened the fermentation period, and decreased the carbon and nitrogen losses during fermentation. With the application of SEOF, the seed germination index of cabbage and tomato was higher than 80% , suggesting that the fertilizer had no inhibitory effect on the seed germination. The application of SEOF not only increased the Chinese cabbage yield and its nutrients and Vc contents, decreased the plant nitrate content, but also improved the soil pH value, and increased the soil available nutrients and organic matter contents and soil enzyme activities, with better effect than applying composted goat feces.
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[Transmasseter approach to mid-and-low condylar fractures by mini-retromandibular access].
Hua Xi Kou Qiang Yi Xue Za Zhi
PUBLISHED: 07-23-2011
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To explore a new access for internal rigid fixation of mandibular mid-and-low condylar fracture.
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[Retrospective analysis of 2461 patients with maxillofacial fractures].
Zhonghua Kou Qiang Yi Xue Za Zhi
PUBLISHED: 05-18-2011
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To review the 2461 cases of maxillofacial fractures.
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Diaqua-bis-(2-oxo-2H-chromene-3-carboxyl-ato)copper(II).
Acta Crystallogr Sect E Struct Rep Online
PUBLISHED: 04-28-2011
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In the title compound, [Cu(C(10)H(5)O(4))(2)(H(2)O)(2)], the Cu(II) atom lies on a crystallographic inversion center and exhibits an octa-hedral coordination defined by two O atoms from water mol-ecules in the axial positions and by four O atoms from two deprotonated coumarin-3-carb-oxy-lic acid ligands in the equatorial positions. The angles around the Cu(II) atom vary between 85.32?(6) and 94.68?(6)°. The Cu-O bond distances between the Cu(II) atom and the O atoms vary between 1.9424?(14) and 2.3229?(15)?Å. The layers inter-digitate via face-to-face aromatic inter-actions [3.6490?(8)?Å] between coumarin moieties such that the inter-layer separation is 10.460?(2)?Å, i.e. the length of the c axis. O-H?O hydrogen bonds between the H atoms of coordinated water mol-ecules and the O atoms of carboxyl-ate groups link the complex mol-ecules into layers parallel to the ab plane.
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Diaqua-bis-(2-oxo-2H-chromene-3-carboxyl-ato-?O,O)manganese(II).
Acta Crystallogr Sect E Struct Rep Online
PUBLISHED: 04-28-2011
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In the title compound, [Mn(C(10)H(5)O(4))(2)(H(2)O)(2)], the Mn(II) atom lies on a crystallographic inversion center and is six-coordinated by two O atoms from water mol-ecules in the axial positions and four O atoms from two deprotonated coumarin-3-carb-oxy-lic acid ligands in the equatorial plane. The overall coordination geometry is slightly distorted octa-hedral. The Mn-O bond distances vary between 2.0931?(12) and 2.2315?(13)?Å. O-H?O hydrogen bonds between the H atoms of coordinated water mol-ecules and the O atoms of the carboxyl-ate groups link the complex mol-ecules into two-dimensional layers parallel to the ab plane.
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[X-ray absorption spectroscopic evidence for the formation of Pb(II) inner-sphere adsorption complexes and precipitates at the alkaline soil-water interface].
Guang Pu Xue Yu Guang Pu Fen Xi
PUBLISHED: 04-23-2011
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Adsorption mechanisms of Pb on soil with high CaCO3 content were investigated by combined batch sorption and X-ray absorption fine structure (XAFS). Date from the batch equilibrium studies showed that Pb sorption was nonlinear and was well fitted to Langmiur isotherm. The XAFS data indicated that Pb could be adsorbed via the inner-sphere complex, the precipitation of calcium carbonate containing Pb (PbCaCO3), and outer-sphere Pb sorption complex. The formations of inner-sphere complexes and PbCaCO3 implied strong metal interactions with the surfaces the mechanistic reason for the affinity of Pb for CaCO3 as observed in macroscopic studies. At low metal concentration, 500 mg x L(-1) of initial Pb, radial distance of the first-shell Pb-O (R1) was 0.169 2 nm, however, at 1 000 mg x L(-1) of initial Pb, the R1 was 0.166 8 nm. These revealed that the percentage of inner-sphere complexes increased when the initial Pb was increased from 500 to 1 000 mg x L(-1).
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Blockade of persistent sodium currents contributes to the riluzole-induced inhibition of spontaneous activity and oscillations in injured DRG neurons.
PLoS ONE
PUBLISHED: 03-11-2011
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In addition to a fast activating and immediately inactivating inward sodium current, many types of excitable cells possess a noninactivating or slowly inactivating component: the persistent sodium current (I(NaP)). The I(NaP) is found in normal primary sensory neurons where it is mediated by tetrodotoxin-sensitive sodium channels. The dorsal root ganglion (DRG) is the gateway for ectopic impulses that originate in pathological pain signals from the periphery. However, the role of I(NaP) in DRG neurons remains unclear, particularly in neuropathic pain states. Using in vivo recordings from single medium- and large-diameter fibers isolated from the compressed DRG in Sprague-Dawley rats, we show that local application of riluzole, which blocks the I(NaP), also inhibits the spontaneous activity of A-type DRG neurons in a dose-dependent manner. Significantly, riluzole also abolished subthreshold membrane potential oscillations (SMPOs), although DRG neurons still responded to intracellular current injection with a single full-sized spike. In addition, the I(NaP) was enhanced in medium- and large-sized neurons of the compressed DRG, while bath-applied riluzole significantly inhibited the I(NaP) without affecting the transient sodium current (I(NaT)). Taken together, these results demonstrate for the first time that the I(NaP) blocker riluzole selectively inhibits I(NaP) and thereby blocks SMPOs and the ectopic spontaneous activity of injured A-type DRG neurons. This suggests that the I(NaP) of DRG neurons is a potential target for treating neuropathic pain at the peripheral level.
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The protective effects of inosine against chemical hypoxia on cultured rat oligodendrocytes.
Cell. Mol. Neurobiol.
PUBLISHED: 01-20-2011
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Inosine is a purine nucleoside and is considered protective to neural cells including neurons and astrocytes against hypoxic injury. However, whether oligodendrocytes (OLs) could also be protected from hypoxia by inosine is not known. Here we investigated the effects of inosine on primarily cultured rat OLs injured by rotenone-mediated chemical hypoxia, and the mechanisms of the effects using ATP assay, MTT assay, PI-Hoechst staining, TUNEL, and immunocytochemistry. Results showed that rotenone exposure for 24 h caused cell death and impaired viability in both immature and mature OLs, while pretreatment of 10 mM inosine 30 min before rotenone administration significantly reduced cell death and improved the viability of OLs. The same concentration of inosine given 120 min after rotenone exposure also improved viability of injured mature OLs. Immunocytochemistry for nitrotyrosine and cellular ATP content examination indicated that inosine may protect OLs by providing ATP and scavenging peroxynitrite for cells. In addition, immature OLs were more susceptible to hypoxia than mature OLs; and at the similar degree of injury, inosine protected immature and mature OLs differently. Quantitative real-time PCR revealed that expression of adenosine receptors was different between these two stages of OLs. These data suggest that inosine protect OLs from hypoxic injury as an antioxidant and ATP provider, and the protective effects of inosine on OLs vary with cell differentiation, possibly due to the adenosine receptors expression profile. As OLs form myelin in the central nervous system, inosine could be used as a promising drug to treat demyelination-involved disorders.
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Adsorption of diuron and dichlobenil on multiwalled carbon nanotubes as affected by lead.
J. Hazard. Mater.
PUBLISHED: 01-19-2011
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The effect of lead on the adsorption of diuron and dichlobenil on multiwalled carbon nanotubes (MWCNTs) was investigated to explore the possible application of MWCNTs for removal of both herbicides from contaminated water. The adsorption of diuron and dichlobenil on MWCNTs at pH 6 was nonlinear and fit the Polanyi-Manes model well. The adsorption of diuron and dichlobenil was closely correlated with specific surface areas and micropore volumes of MWCNTs. An increase in oxygen content of MWCNTs with same diameters and similar surface areas decreased the adsorption of diuron and dichlobenil, while increased the adsorption of lead. Micro-Fourier transform infrared spectroscopic study indicated that hydrogen bonding is a main mechanism responsible for the adsorption of diuron or dichlobenil onto MWCNTs-O. Oxygen containing groups, mainly carboxylic groups, significantly increased the adsorption of lead through the formations of outer-sphere and inner-sphere complexes, which are verified by X-ray absorption spectroscopic measurements. Oxygen containing groups and the presence of lead diminished the adsorption of diuron and dichlobenil. The suppression mechanisms of lead were ascribed to hydration and complexation of lead with carboxylic groups, which may occupy part of surface of MWCNTs-O. The large hydration shell of lead cations may intrude or shield hydrophobic and hydrophilic sites, resulting in a decreased adsorption of diuron and dichlobenil at the lead-complexed moieties.
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[?(2)-Bis(diphenyl-phosphanyl)methane][?(3)-bis-(diphenyl-phosphanyl)meth-yl]trichlorido-tetra-gold(I) tetra-hydro-furan disolvate.
Acta Crystallogr Sect E Struct Rep Online
PUBLISHED: 01-08-2011
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The title tetra-nuclear complex, [Au(4)(C(25)H(21)P(2))Cl(3)(C(25)H(22)P(2))]·2C(4)H(8)O, features two non-equivalent Ph(2)PCPPh(2) fragments, one of which represents the complete mol-ecule (with two H atoms at the central C atom); each of the two P atoms of this mol-ecule is coordinated by an Au atom [Au-P = 2.2256?(13) and 2.2710?(13)?Å], and these two Au atoms form an Au-Au bond [3.2945?(3)?Å], thus closing the five-membered Au(2)P(2)C ring. The first of these Au atoms has a terminal chlorido ligand [Au-Cl = 2.2806?(12)?Å], whereas the second Au atom forms a covalent bond with the central C atom of the bis-(diphenyl-phosphino)methyl group [Au-C = 2.114?(5)?Å]; the latter group in turn coordinates with its P atoms the gold atoms of the Cl-Au-Au-Cl group [Au-P = 2.2356?(13) and 2.2338?(13), Au-Au = 3.3177?(3), Au-Cl = 2.3091?(12) and 2.2950?(13)?Å], thus closing the second Au(2)P(2)C ring. The two such rings have different chemical functions, but both exhibit envelope conformations. However, the first (with different substituents at the Au atoms) is non-symmetrical with one of the P atoms in the flap position of the envelope; the other one has a conformation with mirror symmetry, and the gold-substituted C atom is displaced by 0.740?(5)?Å from the almost exactly planar (r.m.s. deviation = 0.0038?Å) Au(2)P(2) group.
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[X-ray absorption fine structure (XAFS) study of the effects of pH on Pb(II) sorption by soil].
Guang Pu Xue Yu Guang Pu Fen Xi
PUBLISHED: 12-03-2010
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Combined batch sorption and in situ X-ray absorption fine structure (XAFS) provide direct assessment of the mechanisms for Pb(II) sorption at the soil-water interface under different pH conditions. The XAFS data indicated that the innersphere Pb sorption complex with ionic character (Pb4 (OH)4(4+)) dominated the Pb surface speciation, and the outer-sphere Pb sorption complex and the precipitation of calcium carbonate containing Pb(PbCaCO3) were also involved in the adsorption samples. Coordination number and radial distance of the first-shell Pb-O decreased from 0.172 7 to 0.166 6 nm and the percentage of inner-sphere complexes increased when the initial pH changed from 6.0 to 8.5, indicating that the mechanism of Pb(II) sorption by the soil was pH-dependent.
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Moire? superstructures of graphene on faceted nickel islands.
ACS Nano
PUBLISHED: 10-14-2010
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Using scanning tunneling microscopy and spectroscopy, in combination with density functional theory calculations, we investigated the morphology and electronic structure of monolayer graphene grown on the (111) and (110) facets of three-dimensional nickel islands on highly oriented pyrolytic graphite substrate. We observed graphene domains exhibiting hexagonal and striped moire? patterns with periodicities of 22 and 12 Å, respectively, on (111) and (110) facets of the Ni islands. Graphene domains are also observed to grow, as single crystals, across adjacent facets and over facet boundaries. Scanning tunneling spectroscopy data indicate that the graphene layers are metallic on both Ni(111) and Ni(110), in agreement with the calculations. We attribute this behavior to a strong hybridization between the d-bands on Ni and the ?-bands of carbon. Our findings point to the possibility of preparing large-area epitaxial graphene layers even on polycrystalline Ni substrates.
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Metformin promotes progesterone receptor expression via inhibition of mammalian target of rapamycin (mTOR) in endometrial cancer cells.
J. Steroid Biochem. Mol. Biol.
PUBLISHED: 10-08-2010
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Progesterone has been used in the hormonal treatment of endometrial cancer (EC) for many years, but the response rates are unsatisfying. The down-regulated progesterone receptor (PR) is the main reason for treatment failure. The insulin-like growth factor (IGF) system is related to EC risk, and IGF-I can inhibit PR transcription in breast cancer. Recent evidence suggests that metformin-combined oral contraceptives may reverse progesterone-resistant atypical endometrial hyperplasia, but the mechanism is unclear. We attempt to investigate the interaction of metformin, PR and IGF-II expression, and identify whether metformin can enhance the antitumor effect of medroxyprogesterone acetate (MPA) using Ishikawa and HEC-1B EC cell lines. We found that both IGF-I and IGF-II inhibit PR A/B mRNA and protein expression, whereas metformin markedly promotes PR expression. In parallel, IGF-II increases phosphorylation of AKT and p70S6K, while metformin increases AMPK phosphorylation and decreases p70S6K phosphorylation. The effects of metformin on PR A/B and p70S6K are partially reversed by an AMPK inhibitor. Furthermore, metformin synergistically antiproliferates MPA in two cell lines, with the peak synergy occurring with 10?M metformin combined with 1?M MPA (CI=0.20448 for Ishikawa, CI=0.12801 for HEC-1B). Our results demonstrate that metformin promotes PR expression, which can be inhibited by overexpressed IGF-II in EC. This effect is partially mediated through activating AMPK followed by inhibiting the overactivated mTOR pathway.
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Insight to ternary complexes of co-adsorption of norfloxacin and Cu(II) onto montmorillonite at different pH using EXAFS.
J. Hazard. Mater.
PUBLISHED: 09-07-2010
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Co-adsorption of norfloxacin (Nor) and Cu(II) on montmorillonite at pH 4.5, 7.0 and 9.0 was studied by integrated batch adsorption experiments and extended X-ray absorption fine structure (EXAFS) spectroscopy. Under such pH conditions the dominant species of Nor are cation (Nor(+)), zwitterion (Nor(±)), and anion (Nor(-)), respectively. Results indicated that Nor sorption decreased with an increase of solution pH. The presence of Cu(II) slightly suppressed the Nor(+) sorption at pH 4.5, while increased Nor(±) and Nor(-)sorption on montmorillonite at pH 7.0 and 9.0, respectively. In contrast, Nor increased Cu(II) adsorption at pH 4.5, but had little effect on the adsorption of Cu(II) on montmorillonite at pH 7.0 and 9.0. Spectroscopic results showed that, at pH 4.5, Nor(+) was sorbed on montmorillonite by the formation of outer-sphere montmorillonite-Nor-Cu(II) ternary surface complex. At pH 7.0, montmorillonite-Nor-Cu(II) and montmorillonite-Cu(II)-Nor ternary surface complexes co-exist. At pH 9.0, montmorillonite-Cu(II)-Nor ternary surface complex was likely formed, which was different to Cu(II)(Nor)(2) precipitate of the solution.
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The mechanism of the molecular interaction between cerium (III) and ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco).
Biol Trace Elem Res
PUBLISHED: 09-01-2010
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The mechanism of the molecular interaction between Ce3+, a member of rare earth elements, and Rubisco in vitro is investigated. The carboxylase activity of Rubisco greatly increased under low concentrations of Ce3+ and decreased under high concentrations of Ce3+. The ultraviolet absorption spectra show that the various concentrations of Ce3+ treatment do not shift the characteristic peaks of Rubisco while the characteristic peak intensity of Rubisco increases with increasing Ce3+ concentration. The Rubisco-Ce3+ interactions also do not cause any noticeable change in the ?max of Rubisco fluorescence spectra. However, the fluorescence intensity of Rubisco is found quenched by the addition of Ce3+, which strongly suggests that Ce3+ could directly bind to the Rubisco protein. and the binding sites is estimated to 1.52 per protein. The binding between Ce3+ and Rubisco is also proved by extended X-ray absorption fine-structure essay; Ce3+ coordinated with eight oxygen atoms of Rubisco in first shells and six oxygen atoms in second shells. The results implied that Ce3+ might improve the microenvironment of Rubisco and, in turn, affected the carboxylase capacity of Rubisco greatly.
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Molecularly imprinted polymer microspheres enhanced biodegradation of bisphenol A by acclimated activated sludge.
Water Res.
PUBLISHED: 08-24-2010
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The impacts of bisphenol A- imprinted polymeric microspheres (MIPMs) on the biodegradation of bisphenol A by acclimated activated sludge were studied. Due to the selective adsorption of MIPMs to bisphenol A (BPA) and its analogues, addition of MIPMs to activated sludge increased levels of BPA and its metabolites, which were also the substrates of biodegradation. Higher substrates (BPA and its metabolites) level promoted biodegradation efficiencies of activated sludge via accelerating removal speed of BPA and its metabolites, increasing degradation rate and decreasing half-lives of biodegradation. The enhancement of MIPMs in degradation efficiencies was more significant in environmental water containing low-level of pollutants, and water containing interferences such as heavy metals and humic acid. Furthermore, MIPMs were more suitable than non-selective sorbents such as active carbon to be used as enhancer for BPA biodegradation. MIPMs combined with activated sludge are simple, effective, environmental-friendly processes to biodegrade low-level pollutants in environmental water.
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Large scale pattern graphene electrode for high performance in transparent organic single crystal field-effect transistors.
ACS Nano
PUBLISHED: 06-12-2010
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High quality, large grain size graphene on polycrystalline nickel film on two inch silicon wafers was successfully synthesized by the chemical vapor deposition (CVD) method. The polydimethylsiloxane (PDMS) stamping method was used for graphene transferring in this experiment. The graphene transferred onto Al2O3/ITO substrates was patterned into macroscopic dimension electrodes using conventional lithography followed by oxygen plasma etching. Experimental results show that this graphene can serve as transparent source and drain electrodes in high performance organic semiconductor nanoribbon organic field-effect transistors (OFETs), facilitating high hole injection efficiency due to the preferred work function match with the channel material: single crystalline copper phthalocyanine (CuPc) nanoribbons. The nanoribbons were grown on top of the patterned graphene via evaporate-deposition to form the FET device. The carrier mobility and on/off current ratio of such devices were measured to be as high as 0.36 cm2/(V s) and 10(4).
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[Inhibitory effect of SHP-1 gene transfer on the proliferation of breast cancer cell line MDA-MB-231].
Nan Fang Yi Ke Da Xue Xue Bao
PUBLISHED: 05-27-2010
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To observe SHP-1 protein expression in breast cancer cell line MDA-MB-231 before and after SHP-1 gene transfer and its effect on the proliferation of MDA-MB-231 cells.
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Na-doped p-type ZnO microwires.
J. Am. Chem. Soc.
PUBLISHED: 02-10-2010
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p-Type ZnO microwires were first synthesized by a simple chemical vapor deposition method using Na as the dopant source. p-Type doping was confirmed by the electrical transport in single-wire field-effect transistors and low-temperature photoluminescence. The carrier mobility of the microwires was estimated to be approximately 2.1 cm(2) V(-1) S(-1).
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2,2-[Ethane-1,2-diylbis(sulfanedi-yl)]bis-(pyridine N-oxide).
Acta Crystallogr Sect E Struct Rep Online
PUBLISHED: 11-16-2009
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The tile compound, C(12)H(12)N(2)O(2)S(2), lies on an inversion center. The two pyridyl rings are parallel to each other. The structure is devoid of any classical hydrogen bonds due to lack of appropriate donors and acceptors for such bonds. However, non-classical hydrogen bonds of the types C-H?O and C-H?S stabilize the structure.
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[Effect of cyclic strain on cell morphology, viability and proliferation of human dental pulp cells in vitro].
Shanghai Kou Qiang Yi Xue
PUBLISHED: 09-21-2009
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To investigate the effect of mechanical strain on cell morphology, viability and proliferation of human dental pulp cells in vitro.
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Effects of copper, lead, and cadmium on the sorption of 2,4,6-trichlorophenol onto and desorption from wheat ash and two commercial humic acids.
Environ. Sci. Technol.
PUBLISHED: 09-08-2009
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The effects of copper (Cu2+), lead (Pb2+), and cadmium (Cd2+) on the sorption of 2,4,6-trichlorophenol (TCP) to and desorption from wheat ash and two commercial humic acids were studied. Copper and Pb2+ diminished the sorption of TCP onto all adsorbents, and made desorption of TCP less hysteretic from ash and German humic acids (GeHA), but more hysteretic from Tianjin humic acids (TJHA). Cadmium had little effect on TCP sorption and desorption. Fourier-transform infra red (FTIR) and X-ray absorption spectroscopy (XAS) in conjunction with fluorescence quenching studies provided insights into the mechanisms of TCP sorption and desorption as affected by Cu2+ and Pb2+, indicating that complexation of Cu2+ and Pb2+ was likely via carboxylic, hydroxylic and phenolic groups of ash, TJHA and GeHA, and that theses same functional groups also reacted with TCP during sorption. In contrast, Cd, a "soft acid", had no effect on the adsorption of TCP. Hydration shells of dense water around adsorbed Cu2+ and Pb2+ ions may also compete with TCP for available surface area. Fluorescence quenching of pyrene verified that for TJHA, Cu2+ and Pb2+ promoted the formation of supramolecular associations with interior hydrophobic regions separated from aqueous surroundings by exterior hydrophilic layers.
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A one-dimensional cadmium(II) complex supported by a sulfur-nitro-gen mixed-donor ligand.
Acta Crystallogr Sect E Struct Rep Online
PUBLISHED: 06-07-2009
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In the title compound, catena-poly[cadmium(II)-bis-(?-5-am-ino-1,3,4-thia-diazole-2-thiol-ato)-?(2)N(3):S(2);?(2)S(2):N(3)], [Cd(C(2)H(2)N(3)S(2))(2)](n), the Cd(II) ion is coordinated by two N atoms of the 1,3,4-thia-diazole rings from two ligands and two S atoms of sulfhydryl from two other ligands in a slightly distorted tetra-hedral geometry. The ligands bridge Cd(II) ions, forming one-dimensional chains along [001], which are connected by N-H?N and N-H?S hydrogen bonds into a three-dimensional network.
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Tetra-aqua-bis(3-carboxyl-atopyridine N-oxide-?O)cadmium(II).
Acta Crystallogr Sect E Struct Rep Online
PUBLISHED: 06-05-2009
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In the title complex, [Cd(C(6)H(4)NO(3))(2)(H(2)O)(4)], the Cd(II) atom is situated on a crystallographic centre of inversion. The Cd(II) atom shows a slightly distorted octa-hedral geometry and is coordinated by four O atoms from water mol-ecules and two O atoms from deprotonated carboxyl groups of nicotinic acid N-oxide ligands. The mononuclear complex mol-ecules are linked by O-H?O hydrogen bonds, forming a three-dimensional network structure.
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2,2-[o-Phenylenebis(methylenethio)]bis(pyridine N-oxide).
Acta Crystallogr Sect E Struct Rep Online
PUBLISHED: 05-27-2009
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In the title compound, C(18)H(16)N(2)O(2)S(2), the benzene ring makes dihedral angles of 7.41 and 86.59° with the two outer pyridine N-oxygen rings. Two short intramolecular C-H?S contacts occur. The crystal packing is stabilized by C-H?O hydrogen bonds, C-H?? inter-actions and weak ?-? staking inter-actions [centroid-centroid distance 3.7596?(7)?Å].
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Tetra-?-acetato-bis-[(pyridine N-oxide)copper(II)](Cu-Cu).
Acta Crystallogr Sect E Struct Rep Online
PUBLISHED: 05-26-2009
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The mol-ecule of the title binuclear copper(II) complex, [Cu(2)(CH(3)COO)(4)(C(5)H(5)NO)(2)], occupies a special position on a crystallographic inversion centre; the coordination environment of the Cu(II) atom is slightly distorted square-pyramidal and is made up of four O atoms belonging to four acetate groups in the basal plane with the O atom of pyridine N-oxide ligand in the apical position. The Cu-Cu distance is 2.6376?(6)?Å.
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Bis(5,6-dicarboxy-benzimidazolium) sulfate monohydrate.
Acta Crystallogr Sect E Struct Rep Online
PUBLISHED: 04-29-2009
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In the title compound, 2C(9)H(7)N(2)O(4) (+)·SO(4) (2-)·H(2)O, the sulfate S atom and the water O atom reside on a crystallographic twofold axis. In the crystal, the component species are linked by N-H?O, O-H?O and C-H?O hydrogen bonds, forming a three-dimensional network structure. An intramol-ecular O-H?O link is seen in the cation.
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Adsorption of 2,4,6-trichlorophenol by multi-walled carbon nanotubes as affected by Cu(II).
Water Res.
PUBLISHED: 02-27-2009
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Adsorption equilibrium of 2,4,6-trichlorophenol (TCP) on multi-walled carbon nanotubes (MWCNTs) was investigated to explore the possibility of using MWCNTs for concentration, detection and removal of TCP from contaminated water. The adsorption of TCP on MWCNTs at pH 4 was nonlinear, reversible and best fit by a Polanyi-Manes model. Oxidation treatment increased surface area and introduced hydrophilic carboxylic groups to the defect sites of MWCNTs, hence increased the sorption of TCP and Cu(II) individually. Cu(II) suppressed the sorption of TCP on oxidized MWCNTs15A, but had little effect on as-grown MWCNTs15. TCP had no influence on Cu(II) sorption to either. The mechanisms of Cu(II) suppression effect on TCP adsorption are ascribed to the formation of surface complexes of Cu(II), which was verified by X-ray absorption spectroscopy. Cu(II) exerts a cross-linking effect of functional groups on adjacent tubes, creating a more tightly knit bundle and suppressing the condensation of TCP in the pore spaces between the tubes. The large hydration sphere around surface complexes of Cu(II) may also intrude or shield hydrophilic sites, leading to the "crowding out" of TCP around the Cu(II)-complexed sites.
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[Involvement of cerebral neuroglobin in electroacupuncture preconditioning-induced protection effect in cerebral ischemia-reperfusion rats].
Zhen Ci Yan Jiu
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To observe the effect of electroacupuncture (EA) preconditioning on cerebral ischemia and the role of cerebral neuroglobin (NgB) in EA-induced brain protection in focal cerebral ischemia and reperfusion injury (CI/RI) rats.
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[Changes of CK-MB and HSP 60 in electrical-injuried rats].
Fa Yi Xue Za Zhi
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To investigate the changes of creatine kinase-MB (CK-MB) and heat shock protein 60 (HSP 60) in rats without electric marks after electric injury, to identify the relationship of the CK-MB, HSP 60 and the time of electric injuries, and to evaluate the damage to cells after electric injury.
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Fast determination of trace dimethyl fumarate in milk with near infrared spectroscopy following fluidized bed enrichment.
Anal Bioanal Chem
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Near infrared spectroscopy (NIRS) has been proved to be a powerful analytical tool in different fields. However, because of the low sensitivity in near infrared region, it is a significant challenge to detect trace analytes with normal NIRS technique. A novel enrichment technique called fluidized bed enrichment has been developed recently to improve sensitivity of NIRS which allows a large volume solution to pass through within a short time. In this paper, fluidized bed enrichment method was applied in the determination of trace dimethyl fumarate in milk. Macroporous styrene resin HZ-816 was used as adsorbent material, and 1 L solution of dimethyl fumarate was run to pass through the material for concentration. The milk sample was pretreated to remove interference matters such as protein, fat, and then passed through the material for enrichment; after that, diffuse reflection NIR spectra were measured for the analyte concentrated on the material directly without any elution process. The enrichment and spectral measurement procedures were easy to operate. NIR spectra in 900-1,700 nm were collected for dimethyl fumarate solutions in the concentration range of 0.506-5.060 ?g/mL and then used for multivariate calibration with partial least squares (PLS) regression. Spectral pretreatment methods such as multiplicative scatter correction, first derivative, second derivative, and their combinations were carried out to select the optimal PLS model. Root mean square error of cross-validation calculated by leave-one-out cross-validation is 0.430 ?g/mL with ten PLS factors. Ten samples in an independent test set were predicted by the model with the mean relative error of 5.33%. From the results shown in this work, it can be concluded that the NIR technique coupled with on-line enrichment method can be expanded for the determination of trace analytes, and its applications in real liquid samples like milk and juice may also be feasible.
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Facile synthesis of dispirooxindole-fused heterocycles via domino 1,4-dipolar addition and Diels-Alder reaction of in situ generated Huisgen 1,4-dipoles.
Org. Lett.
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A facile synthetic protocol was developed for the efficient synthesis of complex dispirooxindole-fused heterocycles via a three-component reaction. The key strategies involve a domino 1,4-dipolar addition and Diels-Alder reaction of the in situ generated Huisgen 1,4-dipoles from the addition reaction of 4-dimethylaminopyridine with acetylenedicarboxylate to 3-phenacylideneoxindole.
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The use of copper(II) isonicotinate-based micro-solid-phase extraction for the analysis of polybrominated diphenyl ethers in soils.
Anal. Chim. Acta
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A micro-solid-phase extraction (?-SPE) device was developed by filling copper(II) isonicotinate coordination polymer (Cu(4-C(5)H(4)N-COO)(2)(H(2)O)(4)) into a porous polypropylene envelope, and the ?-SPE, coupling with gas chromatography (GC) with a micro-cell electron capture detector (?-ECD), was used for extraction and determination of PBDEs in soils. Variables affecting extraction procedures, including temperature, water volume, extraction time, and desorption time, were investigated in a spiked soil, and the parameters were optimized. Under the optimal experimental conditions, the method detection limits for seven PBDEs (BDE-28, 47, 99, 100, 153, 154, and 183) were in the range of 0.026-0.066 ng g(-1), and the reproducibility was satisfactory with the relative standard deviation in range of 1.3-10.1%. Good linear relationship between PBDEs concentrations and GC signals (defined as peak area) was obtained in the range between 0.1 and 200 ng g(-1). The recovery of the seven PBDEs by ?-SPE varied from 70 to 90%, which was comparable to that determined by accelerated solvent extraction method. Finally, the proposed method was used to determine PBDEs in several field-contaminated soils, and it was suggested that the ?-SPE is a promising alternative microextraction technique for the detection of PBDEs in soils.
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Application of portable gas chromatography-photo ionization detector combined with headspace sampling for field analysis of benzene, toluene, ethylbenzene, and xylene in soils.
Environ Monit Assess
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A method based on headspace (HS) sampling coupling with portable gas chromatography (GC) with photo ionization detector (PID) was developed for rapid determination of benzene, toluene, ethylbenzene, and xylenes (BTEX) in soils. Optimal conditions for HS gas sampling procedure were determined, and the influence of soil organic matter on the recovery of BTEX from soil was investigated using five representative Chinese soils. The results showed that the HS-portable-GC-PID method could be effectively operated at ambient temperature, and the addition of 15 ml of saturated NaCl solution in a 40-ml sampling vial and 60 s of shaking time for sample solution were optimum for the HS gas sampling procedure. The recoveries of each BTEX in soils ranged from 87.2 to 105.1 %, with relative standard deviations varying from 5.3 to 7.8 %. Good linearity was obtained for all BTEX compounds, and the detection limits were in the 0.1 to 0.8 ?g kg(-1) range. Soil organic matter was identified as one of the principal elements that affect the HS gas sampling of BTEX in soils. The HS-portable-GC-PID method was successfully applied for field determination of benzene and toluene in soils of a former chemical plant in Jilin City, northeast China. Considering its satisfactory repeatability and reproducibility and particular suitability to be operated in ambient environment, HS sampling coupling with portable GC-PID is, therefore, recommended to be a suitable screening tool for rapid on-site determination of BTEX in soils.
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[Study on the status and related socio-psychological factors of maternal depression among high-risk pregnancy women in Harbin city].
Zhonghua Yu Fang Yi Xue Za Zhi
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This study aimed to investigate the depression status among high-risk pregnancy women, and to analyze its relevant social and psychological factors.
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Cytosolic phospholipase A2 alpha/arachidonic acid signaling mediates depolarization-induced suppression of excitation in the cerebellum.
PLoS ONE
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Depolarization-induced suppression of excitation (DSE) at parallel fiber-Purkinje cell synapse is an endocannabinoid-mediated short-term retrograde plasticity. Intracellular Ca(2+) elevation is critical for the endocannabinoid production and DSE. Nevertheless, how elevated Ca(2+) leads to DSE is unclear.
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Tetra-aqua-{5-[(pyridin-2-yl-methyl-idene)amino]benzene-1,3-dicarboxyl-ato-?(2)N,N}nickel(II) tetra-hydrate.
Acta Crystallogr Sect E Struct Rep Online
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The title structure, [Ni(C(14)H(8)N(2)O(4))(H(2)O)(4)]·4H(2)O, contains a mononuclear Ni(II) complex formed by a chelating bidentate Schiff base and by four Ni-bonded water mol-ecules. The Ni(II) atom is in a distorted octa-hedral coordination by two N atoms in a cis disposition [Ni-N = 2.0753?(16) and 2.1048?(16)?Å] and by four water O atoms [Ni-O = 2.0500?(15)-2.0822?(15)?Å]. The crystal structure is completed by four further non-coordinating water mol-ecules and all constituents are linked in a three-dimensional manner by an extensive system of 16 O-H?O hydrogen bonds.
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Characterization of LHY-821, a novel moderately differentiated endometrial carcinoma cell line.
In Vitro Cell. Dev. Biol. Anim.
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Endometrial cancer is a major problem for women but only a small number of comprehensively characterized cell models are available for studies. Here, we established a new cell line derived from a Stage IIIc(1) Grade 2 endometrial adenocarcinoma. The cell line, designated LHY-821, was characterized using growth curve, karyotyping, immunohistochemical staining, immunoblotting, drug sensitivity assay, invasion assay, and xenografting in nude mice. LHY-821 has a doubling time of about 46 h and a colony-forming efficiency of approximately 71 %. These cells expresse high levels of progesterone receptor but not estrogen receptor and are sensitive to medroxyprogesterone acetate (MPA). LHY-821 also expresses pan-cytokeratin, PTEN, p53, ?-catenin, IGF-1, and IGF-2. In addition, karyotype analysis revealed that LHY-821 possessed a near diploid karyotype including 6q-, 10p-, Xq-, 13q+, 17p+, and Triplo-12. LHY-821 showed highly tumorigenicity in nude mice (100 %) and weak invasiveness. Chemosensitivity tests showed that LHY-821 was sensitive to both carboplatin and paclitaxel. LHY-821 is an immortalized cell line which had survived more than 80 serial passages; it may provide a novel tool to study the molecular mechanism and potential treatment for endometrial cancer.
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JoVE Visualize is a tool created to match the last 5 years of PubMed publications to methods in JoVE's video library.

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