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Find video protocols related to scientific articles indexed in Pubmed.
High-Precision (143)Nd/(144)Nd Ratios from NdO(+) Data Corrected with in-Run Measured Oxygen Isotope Ratios.
Anal. Chem.
PUBLISHED: 10-27-2014
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The NdO(+) technique has been considerably refined in recent years for high-precision measurement of Nd isotope ratios in low-level samples (1-5 ng Nd). As oxygen isotopic compositions may vary significantly with experimental conditions such as filament material, ionization enhancer and the ambient oxygen in the ion source, great "care" should be taken for using correct oxygen isotopic compositions to do the isobaric oxide corrections for the "conventional" NdO(+) method. Our method presented here for NdO(+) data reduction and PrO(+) interference corrections uses the oxygen isotope composition determined in each cycle of the NdO(+) measurements. For that purpose, we measured the small ion signals of (150)Nd(17)O(+) and (150)Nd(18)O(+) with amplifiers equipped with 10(12) ? feedback resistors, and those of Nd(16)O(+) ion beams with 10(11) ? amplifiers. Using 10(12) ? amplifiers facilitates a precise measurement of the very small (150)Nd(17)O(+) and (150)Nd(18)O(+) ion signals and calculation of highly accurate and precise (143)Nd/(144)Nd isotope ratios. The (143)Nd/(144)Nd ratios for JNdi-1 standards and several whole-rock reference materials determined with the method on 4 ng of Nd loads are consistent with previously reported values within analytical error, with internal and external precision (2 RSE and 2 RSD) of better than 20 and 30 ppm, respectively.
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Rotationally resolved state-to-state photoionization and photoelectron study of titanium carbide and its cation (TiC/TiC(+)).
J Chem Phys
PUBLISHED: 10-17-2014
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Titanium carbide and its cation (TiC/TiC(+)) have been investigated by the two-color visible (VIS)-ultraviolet (UV) resonance-enhanced photoionization and pulsed field ionization-photoelectron (PFI-PE) methods. Two visible excitation bands for neutral TiC are observed at 16?446 and 16?930 cm(-1). Based on rotational analyses, these bands are assigned as the respective TiC((3)?1) ? TiC(X(3)?(+)) and TiC((3)?(+)) ? TiC(X(3)?(+)) transition bands. This assignment supports that the electronic configuration and term symmetry for the neutral TiC ground state are …7?(2)8?(1)9?(1)3?(4) (X(3)?(+)). The rotational constant and the corresponding bond distance of TiC(X(3)?(+); v? = 0) are determined to be B0? = 0.6112(10) cm(-1) and r0? = 1.695(2) Å, respectively. The rotational analyses of the VIS-UV-PFI-PE spectra for the TiC(+)(X; v(+) = 0 and 1) vibrational bands show that the electronic configuration and term symmetry for the ionic TiC(+) ground state are …7?(2)8?(1)3?(4) (X(2)?(+)) with the v(+) = 0 ? 1 vibrational spacing of 870.0(8) cm(-1) and the rotational constants of Be (+) = 0.6322(28) cm(-1), and ?e (+) = 0.0085(28) cm(-1). The latter rotational constants yield the equilibrium bond distance of re (+) = 1.667(4) Å for TiC(+)(X(2)?(+)). The cleanly rotationally resolved VIS-UV-PFI-PE spectra have also provided a highly precise value of 53?200.2(8) cm(-1) [6.5960(1) eV] for the adiabatic ionization energy (IE) of TiC. This IE(TiC) value along with the known IE(Ti) has made possible the determination of the difference between the 0 K bond dissociation energy (D0) of TiC(+)(X(2)?(+)) and that of TiC(X(3)?(+)) to be D0(Ti(+)-C) - D0(Ti-C) = 0.2322(2) eV. Similar to previous experimental observations, the present state-to-state PFI-PE study of the photoionization transitions, TiC(+)(X(2)?(+); v(+) = 0 and 1, N(+)) ? TiC((3)?1; v', J'), reveals a strong decreasing trend for the photoionization cross section as |?N(+)| = |N(+) - J'| is increased. The maximum |?N(+)| change of 7 observed here is also consistent with the previous experimental results for the 3d transition-metal carbides, oxides, and nitrides. However, the VIS-UV-PFI-PE spectra for TiC(+)(X(2)?(+); v(+) = 0 and 1, N(+)) are found to display only the negative ?N(+) (N(+)-J'? 0) transitions, indicating that the cross sections for the formation of positive ?N(+) (N(+)-J' > 0) transitions by both the channel coupling mechanism and direct photoionization are negligibly small.
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[Extracorporeal shockwave therapy for erectile dysfunction and Peyronie's disease].
Zhonghua Nan Ke Xue
PUBLISHED: 10-14-2014
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Penile vascular endothelial damage, vasomotor dysfunction, and blood flow deficiency are the major causes of erectile dysfunction (ED). Current management of ED mostly depends on selective phosphodiesterase type 5- (PDE5) inhibitors, which fail for some ED patients. For Peyronie's disease-induced ED, surgical and physical therapies are used in addition to PDE5I medication, but frequently it is difficult to achieve satisfactory results. Recent studies show that the low-intensity extracorporeal shockwave therapy can promote angiogenesis and improve blood flow to the penis, which promises to be a novel effective therapy for ED and Peyronie's dis-
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Photochemistry. Evidence for direct molecular oxygen production in CO? photodissociation.
Science
PUBLISHED: 10-02-2014
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Photodissociation of carbon dioxide (CO2) has long been assumed to proceed exclusively to carbon monoxide (CO) and oxygen atom (O) primary products. However, recent theoretical calculations suggested that an exit channel to produce C + O2 should also be energetically accessible. Here we report the direct experimental evidence for the C + O2 channel in CO2 photodissociation near the energetic threshold of the C((3)P) + O2(X(3)?(g)(-)) channel with a yield of 5 ± 2% using vacuum ultraviolet laser pump-probe spectroscopy and velocity-map imaging detection of the C((3)PJ) product between 101.5 and 107.2 nanometers. Our results may have implications for nonbiological oxygen production in CO2-heavy atmospheres.
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New dual therapy for primary treatment of Helicobacter pylori infection: A prospective randomized study in Shanghai, China.
J Dig Dis
PUBLISHED: 09-11-2014
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To evaluate the eradication rates, side effects and the patient compliance of a dual therapy with rabeprazole and amoxicillin as the first-line therapy in patients with Helicobacter pylori (H. pylori) infection.
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Visual electrochemiluminescence detection of telomerase activity based on multifunctional Au nanoparticles modified with G-quadruplex deoxyribozyme and luminol.
Chem. Commun. (Camb.)
PUBLISHED: 09-09-2014
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A novel visual electrochemiluminescence (ECL) analysis strategy for detection of telomerase activity is reported on a microarray chip, with G-quadruplex deoxyribozyme (DNAzyme) and luminol modified Au nanoparticles (NPs) as double-catalytic amplification labels.
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Propofol and dexmedetomidine for monitored anesthesia care during laser in situ keratomileusis.
Eye Contact Lens
PUBLISHED: 07-26-2014
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The safety and efficacy of dexmedetomidine during laser in situ keratomileusis (LASIK) in stressed patients under topical anesthesia has not been fully investigated.
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Elevated plasma visfatin levels correlate with conversion of laparoscopic cholecystectomy to open surgery in acute cholecystitis.
Peptides
PUBLISHED: 07-11-2014
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Visfatin correlates with inflammation and its levels in peripheral blood are associated with some inflammatory diseases. This study aimed to assess the relationship between plasma visfatin levels and conversion of laparoscopic cholecystectomy to open surgery in acute cholecystitis. One hundred and forty-six acute cholecystitis patients and 146 sex- and age-matched healthy controls were recruited and their plasma visfatin levels were determined using an enzyme immunoassay. 17 patients (11.6%) underwent conversion. Plasma visfatin levels were statistically significantly elevated in all patients (97.2±41.8ng/mL), those with (161.4±71.3ng/mL) or without conversion (88.7±26.9ng/mL), compared to controls (40.3±13.3ng/mL, all P<0.001). A linear regression analysis showed that plasma visfatin levels were positively associated with plasma C-reactive protein levels (t=0.510, P<0.001). A logistic-regression analysis showed that age [odds ratio (OR) 1.160, 95% confidence interval (CI) 1.011-1.332, P=0.035] and plasma visfatin levels (OR 1.035, 95% CI 1.005-1.066, P=0.022) appeared to be the independent predictors of conversion. A receiver operating characteristic curve analysis found that plasma visfatin levels predicted conversion with high area under curve (AUC) (AUC, 850; 95% CI, 0.781-0.903). The AUC of the visfatin concentration was similar to that of age (AUC, 0.738; 95% CI, 0.659-0.807) (P=0.188). Visfatin improved the AUC of age to 0.914 (95% CI, 0.856-0.954) (P=0.011) using a combined logistic-regression model. Thus, high plasma levels of visfatin are associated with systemic inflammation, and may independently predict conversion of laparoscopic cholecystectomy to open surgery in acute cholecystitis.
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Oxymatrine extracted from Sophora flavescens inhibited cell growth and induced apoptosis in human osteosarcoma MG-63 cells in vitro.
Cell Biochem. Biophys.
PUBLISHED: 07-07-2014
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Oxymatrine, one of the most active components of the ethanol extracts from Sophora flavescens, is known for its potent antitumor activity both in vitro and in vivo. However, the mechanism of its action in mediating the cell apoptosis remains elusive. In this study, we investigated the proliferation inhibitory and apoptotic activities of oxymatrine against human osteosarcoma MG-63 cells. The compound was found to markedly and dose-dependently inhibit the cell proliferation determined by 5-bromo-2-deoxyuridine incorporation. Oxymatrine also induced the cell apoptosis in a dose- and time-dependent manner as showed by the annexin V-FITC/PI double staining and TUNEL assay. Furthermore, a disruption of mitochondrial membrane potential and an up-regulation of cleaved caspases-3, and-9 and downregulation of Bax/Bcl-2 was evidenced in the oxymatrine-treated cells. These proteins have been known to play a pivotal role in the regulation of apoptosis. In conclusion, these observations indicate of the oxymatrine potential as an effective antitumor agent against osteosarcoma. Moreover, the compound appears to exert its anti-tumor action by stimulating the caspase-triggered signaling pathway.
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Baicalin reverse AMPA receptor expression and neuron apoptosis in chronic unpredictable mild stress rats.
Biochem. Biophys. Res. Commun.
PUBLISHED: 07-01-2014
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Changes of a-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptor expression could impacts the viability of neurons and brain levels of brain-derived neurotrophic factor (BDNF) expression in the key brain structures in the pathophysiology of depressive disorder. In the present study, chronic unpredictable mild stress (CUMS) degraded performance decreased AMPA receptor expression and increased neuron apoptosis. Treatment with baicalin (20, 40mg/kg) significantly reversed these changes. This study demonstrates that baicalin has potent antidepressant effect, likely through up-regulated the expression of AMPA receptor and suppression neuron apoptosis in CUMS-treated rats.
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[Advance in saponins of aerial parts of Panax species].
Zhongguo Zhong Yao Za Zhi
PUBLISHED: 06-21-2014
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The bioactivities, chemical composition and distribution of aerial parts of Panax species are different from the roots. The present paper summarized the phytochemical and analytical studies of aerial parts of Panax species, including P. ginseng, P. notoginseng, P. quinquefoliun and P. japonicus. This review aims so as to provide scientific evidences for further investigation of chemical profile, quality control and optimal utilization of these resources.
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Efficacy of glucocorticoids in rodents of severe acute pancreatitis: a meta-analysis.
Int J Clin Exp Pathol
PUBLISHED: 06-15-2014
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The use of corticosteroid in the management of severe acute pancreatitis (SAP) remains contentious and is still being debated despite many pre-clinical studies demonstrating benefits. The limitations of clinical research on corticosteroid in SAP are disparities with regard to benefit, a lack of adequate safety data and insufficient understanding of its mechanisms of action. Thus, we performed a meta-analysis to assess the effectiveness of corticosteroid in experimental SAP and take a closer look at the relation between the animal studies and prospective trials.
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[Analysis of survival and prognostic factors in extremely preterm infants].
Zhongguo Dang Dai Er Ke Za Zhi
PUBLISHED: 06-14-2014
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To investigate the survival rate, complications during hospitalization, and prognostic factors in extremely preterm infants (gestational age less than 28 weeks) in the neonatal intensive care unit (NICU).
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Design, synthesis and biological evaluation of novel 5-phenyl-1H-pyrazole derivatives as potential BRAF(V600E) inhibitors.
Bioorg. Med. Chem.
PUBLISHED: 06-06-2014
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A series of novel 5-phenyl-1H-pyrazole derivatives (5a-5u) containing niacinamide moiety were synthesized and evaluated for biological activity as potential BRAF(V600E) inhibitors. Among them, compound 5h exhibited the most potent inhibitory activity with an IC50 value of 0.33?M for BRAF(V600E). Antiproliferative assay results indicated that compound 5h has better antiproliferative activity against WM266.4 and A375 in vitro with IC50 value of 2.63 and 3.16?M, respectively, being comparable with the positive control vemurafenib. Molecular docking of 5h into the BRAF(V600E) active site was performed to determine the probable binding mode. Furthermore, molecular docking and 3D QSAR study by means of DS 3.5 (Discovery Studio 3.5, Accelrys, Co. Ltd) explored the binding modes and the structure and activity relationship (SAR) of these derivatives.
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N,N'-Dioxide/Nickel(II)-Catalyzed Asymmetric Inverse-Electron-Demand Hetero-Diels-Alder Reaction of ?,?-Unsaturated ?-Ketoesters with Enecarbamates.
Chemistry
PUBLISHED: 05-31-2014
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N,N'-Dioxide/nickel(II) complexes have been developed to catalyze the inverse-electron-demand hetero-Diels-Alder reaction of ?,?-unsaturated ?-ketoesters with acyclic enecarbamates. After detailed screening of the reaction parameters, mild optimized reaction conditions were established, affording 3,4-dihydro-2H-pyranamines in up to 99?% yield, 99?% ee and more than 95:5 d.r. The catalytic system was also efficient for ?-substituted acyclic enecarbamates, affording more challenging 2,3,4-trisubstituted 3,4-dihydro-2H-pyranamine with three contiguous stereogenic centers in excellent yields, diastereoselectivities, and enantioselectivities. The reaction could be scaled up to a gram scale with no deterioration of either enantioselectivity or yield. Based on these experiments and on previous reports, a possible transition state was proposed.
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Narrow band imaging: application for early-stage gastrointestinal neoplasia.
J Dig Dis
PUBLISHED: 05-29-2014
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Narrow-band imaging (NBI) is an imaging technique for endoscopy using optical filters to narrow the bandwidth of spectral transmittance. Through this narrow spectrum, NBI contrasts surface structure and microvascular architecture of various lesions. In this article we focus on the application of NBI for early-stage neoplasia in the esophagus, stomach and colon with a three-step strategy of endoscopic diagnosis: (i) the detection of abnormality; (ii) the differentiation between non-neoplasia and neoplasia; and (iii) staging for tumor extension and depth of invasion.
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Yin Yang-1 suppresses invasion and metastasis of pancreatic ductal adenocarcinoma by downregulating MMP10 in a MUC4/ErbB2/p38/MEF2C-dependent mechanism.
Mol. Cancer
PUBLISHED: 05-26-2014
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Increasing evidence indicates an important role of transcription factor Yin Yang-1 (YY1) in human tumorigenesis. However, its function in cancer remains controversial and the relevance of YY1 to pancreatic ductal adenocarcinoma (PDAC) remains to be clarified.
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Two new acylated flavonol glycosides from the seeds of Lepidium sativum.
Molecules
PUBLISHED: 05-16-2014
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Two new acylated flavonol glycosides named kaempferol-3-O-(2-O-sinapoyl)-?-D-galactopyranosyl-(1 ? 2)-?-D-glucopyranoside-7-O-?-L-rhamnopyranoside (1) and quercetin-3-O-(6-O-benzoyl)-?-D-glucopyranosyl-(1 ? 3)-?-D-galactopyranoside-7-O-?-L-rhamnopyranoside (2), were isolated together with six known compounds from the seeds of L. sativum. Their structures were elucidated on the basis of spectroscopic analysis and chemical methods. In vitro 1 and 2 inhibited nitric oxide production in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells, with IC50 values of 25.36 and 25.08 µM, respectively.
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Norcassane- and cassane-type furanoditerpenoids from the seeds of Caesalpinia sappan.
Fitoterapia
PUBLISHED: 05-04-2014
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Three novel furanoditerpenoids, norcaesalpinin J (1) featuring an unusual 20-norcassane hydroperoxide and phangininoxys B (2) and C (3) possessing cassane hemiketal skeletons, were isolated from the seeds of Caesalpinia sappan. Their structures were elucidated by extensive spectroscopic methods. All isolates were evaluated for the cytotoxic activities on three human cancer cell lines.
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Enhanced dissolution and oral bioavailability of valsartan solid dispersions prepared by a freeze-drying technique using hydrophilic polymers.
Drug Deliv
PUBLISHED: 04-17-2014
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Abstract This study aimed to improve the dissolution rate and oral bioavailability of valsartan (VAL), a poorly soluble drug using solid dispersions (SDs). The SDs were prepared by a freeze-drying technique with polyethylene glycol 6000 (PEG6000) and hydroxypropylmethylcellulose (HPMC 100KV) as hydrophilic polymers, sodium hydroxide (NaOH) as an alkalizer, and poloxamer 188 as a surfactant without using any organic solvents. In vitro dissolution rate and physicochemical properties of the SDs were characterized using the USP paddle method, differential scanning calorimetry (DSC), X-ray diffractometry (XRD) and Fourier transform-infrared (FT-IR) spectroscopy, respectively. In addition, the oral bioavailability of SDs in rats was evaluated by using VAL (pure drug) as a reference. The dissolution rates of the SDs were significantly improved at pH 1.2 and pH 6.8 compared to those of the pure drug. The results from DSC, XRD showed that VAL was molecularly dispersed in the SDs as an amorphous form. The FT-IR results suggested that intermolecular hydrogen bonding had formed between VAL and its carriers. The SDs exhibited significantly higher values of AUC0-24?h and Cmax in comparison with the pure drug. In conclusion, hydrophilic polymer-based SDs prepared by a freeze-drying technique can be a promising method to enhance dissolution rate and oral bioavailability of VAL.
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Furazolidone-based triple and quadruple eradication therapy for Helicobacter pylori infection.
World J. Gastroenterol.
PUBLISHED: 03-16-2014
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To evaluate the efficacy of furazolidone-based triple and quadruple therapy in eradicating Helicobacter pylori (H. pylori) in a multi-center randomized controlled trial.
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Synthesis, cytotoxicity for mimics of catalase: inhibitors of lactate dehydrogenase and hypoxia inducible factor.
Eur J Med Chem
PUBLISHED: 03-12-2014
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Lactate dehydrogenase A (LDH-A) is a potentially important metabolic target for the inhibition of the highly activated glycolysis pathway in cancer cells. Two Mn(II) complexes with ligand containing di(pyridylmethyl) amine and pyrrol-ketone were used to attenuate the activity of LDH-A. The inhibition of the manganese(II) complexes on the proliferation of HepG-2 cells is related to their ability to disproportionate H2O2. Importantly, the synthesized mimic of catalase can decrease the expression of hypoxia inducible factor (HIF-1?) in HepG-2 cells. So we envision that the multifunctional mimics of catalase could attenuate the activity of LDH-A signaling the cancer cells to death through HIF-1? involved path.
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Asiaticoside attenuates memory impairment induced by transient cerebral ischemia-reperfusion in mice through anti-inflammatory mechanism.
Pharmacol. Biochem. Behav.
PUBLISHED: 03-04-2014
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Asiaticoside (AS) is isolated from Centella asiatica (L.) which has been using for a long time as a memory enhancing drug in India. This study was to investigate the effects of AS on memory impairment and inflammatory cytokines expression induced by transient cerebral ischemia and reperfusion in mice, as well as the potential signaling pathway. Transient bilateral common carotid artery occlusion (tBCCAO) induced severe memory deficits in mice according to the Morris water maze task and the step-down passive avoidance test. Meanwhile the microglial activation and the gene expression of inflammatory cytokines including interleukin (IL)-1?, interleukin (IL)-6 and tumor necrosis factor (TNF)-? were increased in the hippocampus of the mice with cerebral ischemia and reperfusion. Oral administration of AS (40 and 60 mg/kg, once per day, started the day after surgery and lasted for 7 days) significantly ameliorated the memory impairment and the inflammation. Moreover, AS (20, 40 and 60 mg/kg) markedly reduced the microglial overactivation and the phosphorylation of p38 MAPK in hippocampus compared with the transient cerebral ischemia and reperfusion group. These results suggested that AS showed the neuroprotective effect against transient cerebral ischemia and reperfusion in mice, and this effect might be associated with the anti-inflammation effect of AS via inhibiting overactivation of p38 MAPK pathway.
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Formulation and in vitro characterization of novel sildenafil citrate-loaded polyvinyl alcohol-polyethylene glycol graft copolymer-based orally dissolving films.
Int J Pharm
PUBLISHED: 02-22-2014
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This work was aimed to develop novel sildenafil citrate (SC)-loaded polyvinyl alcohol (PVA)-polyethylene glycol (PEG) graft copolymer (Kollicoat(®) IR)-based orally dissolving films (ODFs) using a solvent casting method. Formulation factors such as plasticizers and disintegrants were optimized on the basis of characteristics of blank ODFs. The SC-loaded ODF with a loading capacity up to 6.25mg in an area of 6 cm(2) was prepared and evaluated in terms of mechanical properties, disintegration time and dissolution rate. The physicochemical properties of drug-loaded ODF were also investigated using the scanning electron microscope (SEM), X-ray diffraction (XRD), differential scanning calorimetry (DSC) and Fourier transform infrared spectroscopy (FT-IR). The blank ODF composed of Kollicoat(®) IR, sodium alginate (ALG-Na) and glycerol (10:2:1.5, w/w) had a remarkably short disintegration time of about 20s. The SC-loaded ODF showed a delayed disintegration time (about 25s), but exhibited improved mechanical properties when compared to the blank ODF. SC was homogeneously dispersed throughout the ODF and the crystalline form of drug had been partly changed, existing strong hydrogen bonding between the drug and carriers. The Kollicoat(®) IR/ALG-Na based ODFs containing SC might be an alternative to conventional tablet for the treatment of male erectile dysfunction.
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Discrimination of leaves of Panax ginseng and P. quinquefolius by ultra high performance liquid chromatography quadrupole/time-of-flight mass spectrometry based metabolomics approach.
J Pharm Biomed Anal
PUBLISHED: 02-08-2014
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In present study, an ultra high performance liquid chromatography/quadrupole time-of-flight mass spectrometry (UHPLC-QTOF-MS/MS) based metabolomics approach was established to investigate the metabolic profiles and characteristic chemical markers for distinguishing between leaves of Panax ginseng (LPG) and Panax quinquefolius (LPQ). The UHPLC-QTOF-MS/MS data were subjected to principal component analysis (PCA) and orthogonal partial least squared discrimination analysis (OPLS-DA) to rapidly find the potential characteristic components of LPG and LPQ, and the identities of detected peaks including the potential characteristic components were elucidated. Totally, 86 components were identified from these 2 kinds of leaf samples, in which 9 ginsenosides could be regarded as the characteristic chemical markers for the discrimination of LPG from LPQ. These results suggested that UHPLC-QTOF-MS/MS based metabolomics approach is a powerful tool to rapidly find characteristic markers for the quality control of LPG.
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Sleep architecture in school-aged children with primary snoring.
Sleep Med.
PUBLISHED: 01-28-2014
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We aimed to examine if sleep architecture was altered in school-aged children with primary snoring (PS).
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Missing lead and high ³He/?He in ancient sulfides associated with continental crust formation.
Sci Rep
PUBLISHED: 01-21-2014
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Major terrestrial reservoirs have Pb isotopes more radiogenic than the bulk silicate Earth. This requires a missing unradiogenic Pb reservoir, which has been argued to reside in the lower continental crust or dissolved in the core. Chalcophile element studies indicate that continent formation requires the formation of sulfide-bearing mafic cumulates in arcs. Because Pb, but not U, partitions into sulfides, we show that continent formation must have simultaneously generated time-integrated unradiogenic Pb reservoirs composed of sulfide-bearing cumulates, now recycled back into the mantle or stored deep in the continental lithosphere. The generation of such cumulates could also lead to coupled He-Pb isotopic systematics because (4)He is also produced during U-Th-Pb decay. Here, we show that He may be soluble in sulfide melts, such that sulfide-bearing cumulates would be enriched in both Pb and He relative to U and Th, "freezing" in He and Pb isotopes of the ambient mantle at the time of sulfide formation. This implies that ancient sulfide-bearing cumulates would be characterized by unradiogenic Pb and He isotopes (high-(3)He/(4)He). These primitive signatures are usually attributed to primordial, undifferentiated mantle, but in this case, they are the very imprint of mantle differentiation via continent formation.
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Benign perivascular myoid cell tumor (myopericytoma) of the urinary tract: a report of 2 cases with an emphasis on differential diagnosis.
Hum. Pathol.
PUBLISHED: 01-21-2014
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Myopericytoma is a benign mesenchymal neoplasm thought to comprise part of a spectrum of perivascular myoid cell neoplasms with myofibroma, angioleiomyoma, and glomus tumor. We describe 2 such neoplasms involving the urinary tract: 1 incidentally identified in the kidney of a 59-year-old woman and 1 in the urinary bladder of a 52-year-old woman who presented with urinary frequency and dysuria. Histologically, the bladder tumor was composed of numerous blood vessels surrounded by plump perivascular myoid cells, as in subcutaneous myopericytoma. The renal tumor showed similar morphology centrally and a symplastic glomus tumor-like growth pattern at the periphery. Immunohistochemically, both tumors were reactive for markers of smooth muscle differentiation, such as smooth muscle actin and caldesmon/calponin but negative for CD34, cathepsin K, and S100 protein. Both patients are free of disease 14 and 39 months after resection, respectively. Our findings broaden the morphologic spectrum of myopericytoma.
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Asymmetric N-H insertion of secondary and primary anilines under the catalysis of palladium and chiral guanidine derivatives.
Angew. Chem. Int. Ed. Engl.
PUBLISHED: 01-08-2014
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Efficient enantioselective N?H insertion reactions of secondary and primary anilines were catalyzed by palladium(0) in combination with chiral guanidine derivatives. A broad range of substituted anilines were tolerated, and the corresponding products were obtained in high yield (up to 99?%) with good enantioselectivity (up to 94?%?ee) under mild reaction conditions. The N?H insertion mechanism was examined by the study of kinetic isotope effects, control experiments, HRMS, and spectroscopic analysis.
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[An investigation into the Jin xiao fang (Prescriptions of Instant Effect), a lost recipe book of the Tang Dynasty].
Zhonghua Yi Shi Za Zhi
PUBLISHED: 12-19-2013
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Altogether 141 prescriptions in the Jin xiao fang (Prescriptions of Instant Effect), a lost recipe book of the Tang Dynasty are cited in the Wai tai mi yao (Medical Secrets of An Official). Based on the analysis of these citations, it can be sure that the Jin xiao fang was written by Li Jian-yi of the Tang Dynasty, and completed around 724 to 733. These cited prescriptions are related to the clinical subjects, with complicate origins, for example, physicians and scholars. It is characterized by its "xiao yan (efficacy)".
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[Small needle-knife for the treatment of tennis elbow according to its classification].
Zhongguo Gu Shang
PUBLISHED: 11-26-2013
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To observe clinical effect of different treatments for tennis elbow with small needle-knife according to its classification.
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Precise Determination of the Lutetium Isotopic Composition in Rocks and Minerals Using Multicollector ICPMS.
Anal. Chem.
PUBLISHED: 11-11-2013
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Evidence of (176)Hf excess in select meteorites older than 4556Ma was suggested to be caused by excitation of long-lived natural radionuclide (176)Lu to its short-lived isomer (176m)Lu, due to an irradiation event during accretion in the early solar system. A result of this process would be a deficit in (176)Lu in irradiated samples by between 1‰ and 7‰. Previous measurements of the Lu isotope ratio in rock samples have not been of sufficient precision to resolve such a phenomenon. We present a new analytical technique designed to measure the (176)Lu/(175)Lu isotope ratio in rock samples to a precision of ?0.1‰ using a multicollector inductively coupled mass spectrometer (MC-ICPMS). To account for mass bias we normalized all unknowns to Ames Lu. To correct for any drift and instability associated with mass bias, all standards and samples are doped with W metal and normalized to the nominal W isotopic composition. Any instability in the mass bias is then corrected by characterizing the relationship between the fractionation factor of Lu and W, which is calculated at the start of every analytical session. After correction for isobaric interferences, in particular (176)Yb, we were able to measure (176)Lu/(175)Lu ratios in samples to a precision of ?0.1‰. However, these terrestrial standards were fractionated from Ames Lu by an average of 1.22 ± 0.09‰. This offset in (176)Lu/(175)Lu is probably caused by isotopic fractionation of Lu during industrial processing of the Ames Lu standard. To allow more straightforward data comparison we propose the use of NIST3130a as a bracketing standard in future studies. Relative to NIST3130a, the terrestrial standards have a final weighted mean ?(176)Lu value of 0.11 ± 0.09‰. All samples have uncertainties of better than 0.11‰; hence, our technique is fully capable of resolving any differences in ?(176)Lu of greater than 1‰.
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[The comparison of different clinical scoring systems for predicting prognosis in acute pancreatitis based on the revised Atlanta classification].
Zhonghua Nei Ke Za Zhi
PUBLISHED: 11-09-2013
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To compare the predictive value of BISAP (bedside index for severity in acute pancreatitis), APACHE II(acute physiology and chronic health evaluation II), and Ranson scoring system in persistent organ failure(POF) and mortality in patients diagnosed as acute pancreatitis (AP) based on the revised Atlanta classification.
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Chelyabinsk airburst, damage assessment, meteorite recovery, and characterization.
Science
PUBLISHED: 11-07-2013
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The asteroid impact near the Russian city of Chelyabinsk on 15 February 2013 was the largest airburst on Earth since the 1908 Tunguska event, causing a natural disaster in an area with a population exceeding one million. Because it occurred in an era with modern consumer electronics, field sensors, and laboratory techniques, unprecedented measurements were made of the impact event and the meteoroid that caused it. Here, we document the account of what happened, as understood now, using comprehensive data obtained from astronomy, planetary science, geophysics, meteorology, meteoritics, and cosmochemistry and from social science surveys. A good understanding of the Chelyabinsk incident provides an opportunity to calibrate the event, with implications for the study of near-Earth objects and developing hazard mitigation strategies for planetary protection.
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Preparation of three-dimensional graphene oxide-polyethylenimine porous materials as dye and gas adsorbents.
ACS Appl Mater Interfaces
PUBLISHED: 09-12-2013
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We report a facile method for the fabrication of three-dimensional (3D) porous materials via the interaction between graphene oxide (GO) sheets and polyethylenimine (PEI) with high amine density at room temperature under atmospheric pressure without stirring. The structural and physical properties of GO-PEI porous materials (GEPMs) are investigated by scanning electron microscopy, X-ray diffraction, thermogravimetric analysis, and nitrogen adsorption-desorption measurement and their chemical properties are analyzed by X-ray photoelectron spectroscopy, infrared spectroscopy, and Raman spectroscopy. GEPMs possess low density and hierarchical morphology with large specific surface area, and big pore volume. Furthermore, the as-prepared 3D porous materials show an excellent adsorption capacity for acidic dyes on the basis of the pore-rich and amine-rich graphene structure. GEPMs exhibit an extremely high adsorption capacity for amaranth (800 mg g(-1)), which are superior to other carbon materials. In addition, GEPMs also exhibit good adsorption capacity for carbon dioxide (11.2 wt % at 1.0 bar and 273 K).
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A genetic variant in the promoter region of miR-34b/c is associated with a reduced risk of colorectal cancer.
Biol. Chem.
PUBLISHED: 08-30-2013
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The miR-34 family members, described as potential tumor suppressors, were downregulated in colorectal cancer (CRC). Loss of miR-34 impairs TP53-mediated cell death, while overexpression of miR-34 induces apoptosis. A potentially functional polymorphism (i.e., rs4938723T/C) in the promoter region of pri-miR-34b/c was predicted to influence the GATA-X binding sites. We aimed to investigate the association between miR-34b/c rs4938723 and TP53 Arg72Pro polymorphisms and the risk of CRC. We genotyped the two polymorphisms in 347 CRC patients and 488 healthy controls using polymerase chain reaction-restriction fragment length polymorphism and DNA sequencing assay. We found that the CC genotype and C allele of the miR-34b/c rs4938723 were associated with a significantly decreased risk of CRC compared with the TT genotype and T allele (CC vs. TT: adjusted OR=0.56; 95% CI, 0.34-0.91; C vs. T: adjusted OR=0.78; 95% CI, 0.64-0.97). In combined analysis, a borderline significance was also observed in subjects carrying the rs4938723 CT/CC and TP53 GG genotypes (adjusted OR=0.66; 95% CI, 0.43-0.99). These findings indicate that the rs4938723 in the promoter region of pri-miR-34b/c was a protective factor for the development of CRC. As the significance is marginal, further replication studies are warranted to confirm these results.
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Comprehensive analysis of the percentage of surface receptors and cytotoxic granules positive natural killer cells in patients with pancreatic cancer, gastric cancer, and colorectal cancer.
J Transl Med
PUBLISHED: 07-30-2013
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Digestive malignancies, especially pancreatic cancer (PC), gastric cancer (GC), and colorectal cancer (CRC), still occur at persistently high rates, and disease progression in these cancers has been associated with tumor immunosurveillance escape. Natural killer (NK) cell dysfunction may be responsible for this phenomenon, however, the exact relationship between tumor immunosurveillance escape in digestive malignancies and NK cell dysfunction remains unclear.
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[Influence of non-sodium restricted diet with diuretics on plasma rennin, renal blood flow and in patients with cirrhotic ascites].
Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi
PUBLISHED: 07-17-2013
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To explore influence of sodium restricted diet and non-sodium restricted diet on plasma rennin (PRA), angiotensin II (All), ALD, renal blood flow (RBF) and subside of ascites in patients with cirrhotic ascites.
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Natural history of primary snoring in school-aged children: a 4-year follow-up study.
Chest
PUBLISHED: 07-09-2013
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The objective of this study was to examine the natural history of childhood primary snoring (PS) and to identify predictive clinical symptoms and risk factors associated with PS progression to obstructive sleep apnea (OSA).
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LRIG1 dictates the chemo-sensitivity of temozolomide (TMZ) in U251 glioblastoma cells via down-regulation of EGFR/topoisomerase-2/Bcl-2.
Biochem. Biophys. Res. Commun.
PUBLISHED: 06-28-2013
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In the current study, we aimed to understand the potential role of leucine-rich repeats and immunoglobulin-like domains 1 (LRIG1) in TMZ-resistance of U251 glioma cells. We established TMZ-resistant U251 clones (U251/TMZ cells), which expressed low level of LRIG1, but high levels of epidermal growth factor receptor (EGFR), topoisomerase-2 (Topo-2) and Bcl-2. Depletion of LRIG1 by the targeted RNA interference (RNAi) upregulated EGFR/Topo-2/Bcl-2 in U251 cells, and the cells were resistant to TMZ. Reversely, over-expression of LRIG1 in U251 cells downregulated EGFR/Topo-2/Bcl-2 expressions, and cells were hyper-sensitive to TMZ. Our data suggested EGFR-dependent mammalian target of rapamycin (mTOR) activation was important for Topo-2 and Bcl-2 expressions in U251/TMZ cells. The EGFR inhibitor and the mTOR inhibitor downregulated Topo-2/Bcl-2 expressions, both inhibitors also restored TMZ sensitivity in U251/TMZ cells. Finally, inhibition of Topo-2 or Bcl-2 by targeted RNAi(s) knockdown or by the corresponding inhibitor re-sensitized U251/TMZ cells to TMZ, indicating that both Topo-2 and Bcl-2 were important for TMZ resistance in the resistant U251 cells. Based on these results, we concluded that LRIG1 inhibits EGFR expression and the downstream signaling activation, interferes with Bcl-2/Topo-2 expressions and eventually sensitizes glioma cells to TMZ.
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[Role of ubiquitin ligase Ring2 in DNA damage induced by benzo[a]pyrene].
Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi
PUBLISHED: 06-28-2013
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To investigate the role of ubiquitin ligase Ring2 in the DNA damage induced by benzo[a]pyrene (B[a]P).
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RACK1 modulates NF-?B activation by interfering with the interaction between TRAF2 and the IKK complex.
Cell Res.
PUBLISHED: 06-27-2013
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The transcription factor NF-?B plays a pivotal role in innate immunity in response to a variety of stimuli, and the coordinated regulation of this pathway determines the proper host responses to extracellular signals. In this study, we identified RACK1 as a novel negative regulator of NF-?B signaling, NF-?B-mediated cytokine induction and inflammatory reactions. RACK1 physically associates with the IKK complex in a TNF-triggered manner. This interaction interferes with the recruitment of the IKK complex to TRAF2, which is a critical step for IKK phosphorylation and subsequent activation triggered by TNF. By modulating the interaction between TRAF2 and IKK, RACK1 regulates the levels of NF-?B activation in response to different intensities of stimuli. Our findings suggest that RACK1 plays an important role in controlling the sensitivity of TNF-triggered NF-?B signaling by regulating IKK activation and provide new insight into the negative regulation of inflammatory reactions.Cell Research advance online publication 10 December 2013; doi:10.1038/cr.2013.162.
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Organocatalytic oxyamination of azlactones: kinetic resolution of oxaziridines and asymmetric synthesis of oxazolin-4-ones.
J. Am. Chem. Soc.
PUBLISHED: 06-25-2013
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The first example of oxyamination of azlactones with oxaziridines was realized using a chiral bisguanidinium salt. Efficient catalytic asymmetric oxyamination and kinetic resolution of oxaziridines occurred simultaneously. Various chiral oxazolin-4-one derivatives with potential biological activity were obtained (up to 92% ee). Meanwhile, a series of optically pure oxaziridines were recovered with up to 99% ee and successfully used in the asymmetric oxyamination of 3-methyl-1H-indole and styrene. The triple stereodifferentiation process was also studied via control experiments.
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Chiral-Zn(NTf2)2-complex-catalyzed diastereo- and enantioselective direct conjugate addition of arylacetonitriles to alkylidene malonates.
Chemistry
PUBLISHED: 06-04-2013
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Chiral N,N-dioxide/Zn(NTf2)2 complexes were demonstrated to be highly effective in the direct asymmetric conjugate addition of arylacetonitriles to alkylidene malonates under mild conditions. A wide range of substrates were tolerated to afford their corresponding products in moderate-to-good yields with high diastereoselectivities (82:18->99:1 d.r.) and enantioselectivities (81-99% ee). The reactions performed well, owing to the high Lewis acidity of the metal triflimidate and a ligand-acceleration effect. The N,N-dioxide also benefited the deprotonation process as a Brønsted base. The catalytic reaction could be performed on the gram-scale with retention of yield, diastereoselectivity, and enantioselectivity. The products that contained functional groups were ready for further manipulation. In addition, a possible catalytic model was proposed to explain the origin of the asymmetric induction.
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[Identification of risk factors related to the failure of immunization to interrupt hepatitis B virus perinatal transmission].
Zhonghua Gan Zang Bing Za Zhi
PUBLISHED: 05-14-2013
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To explore the factors influencing failure of an immunization to interrupt perinatal (mother-to-child) transmission of hepatitis B virus (HBV).
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Development of strategies for screening, predicting, and diagnosing intrauterine HBV infection in infants born to HBsAg positive mothers.
J. Med. Virol.
PUBLISHED: 05-13-2013
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This study aimed at developing strategies for screening, predicting, and diagnosing intrauterine HBV infection in infants born to HBsAg positive mothers. A total of 1,360 infants born to 1,355 HBsAg positive mothers were followed for 1 year. All newborn infants received active and passive immunization within 24 hr after birth. Maternal and infant blood samples were collected and tested for the status of serum HBsAg, HBeAg, and HBV DNA positivity. The accuracy of infant HBsAg positivity, HBV DNA positivity, HBsAg and HBV DNA double positivity, and HBsAg and/or HBV DNA positivity at birth in the diagnosis of intrauterine HBV infection was evaluated by receiver operating characteristic curve analysis. Of 1,360 infants, 145 tested positive for HBsAg and/or HBV DNA at birth. Twenty-one (1.5%) infants, who were diagnosed with intrauterine HBV infection, showed HBsAg positivity from birth to 7 and 12 months of age. Infant HBsAg positivity at birth had the highest sensitivity in predicting intrauterine HBV infection, while neonatal HBsAg and HBV DNA double positivity had the highest specificity. These findings suggest that infants, who were born to HBsAg positive mothers and were positive for both HBsAg and HBV DNA at birth, may be at a higher risk for intrauterine HBV infection. HBsAg positivity at birth may be a good marker for screening intrauterine HBV infection. Infant HBsAg positivity both at birth and 7 months of age may be used as a diagnostic criterion to simplify diagnostic procedures and improve diagnostic efficiency.
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Inhibition of corneal fibrosis by Smad7 in rats after photorefractive keratectomy.
Chin. Med. J.
PUBLISHED: 04-19-2013
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Haze or corneal subepithelial fibrosis is one of the common complications after refractive surgery procedures, such as photorefractive keratectomy (PRK), laser epithelial keratomileusis, and epipolis laser in situ keratomileusis, which would result in refractive regression, decreased visual quality, and corneal opacification. Haze directly resulted from corneal fibrosis mediated by transforming growth factor ? (TGF?). Smad7, an inhibitory Smad, can inhibit TGF? signal transduction. Recently, the effects of Smad7 on the inhibition of fibrosis in several organs have been studied, while little is known about the effects on cornea after PRK. This study was aimed to determine the effects of lentiviral-mediated Smad7 gene expression on corneal fibrosis in rats after PRK.
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PAX8 expression in sporadic hemangioblastoma of the kidney supports a primary renal cell lineage: implications for differential diagnosis.
Hum. Pathol.
PUBLISHED: 04-15-2013
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Hemangioblastoma is a benign, morphologically distinctive neoplasm of disputed histogenesis that typically occurs in the central nervous system either in the setting of von Hippel-Lindau disease or more often sporadically. Extraneural hemangioblastoma is exceptional and raises a challenging differential diagnosis. Herein, we report a primary renal hemangioblastoma occurring in 51-year-old woman without stigmata of von Hippel-Lindau disease. Histologically, the tumor was composed of sheets of polygonal epithelioid stromal cells with ample pale or eosinophilic, vacuolated cytoplasm in an arborizing capillary network. Tumor cells showed variable nuclear pleomorphism, intranuclear cytoplasmic invaginations, scattered hyaline globules, and psammoma-like calcifications. Some areas showed branching hemangiopericytoma-like vessels with tumor cells radiating from the wall, while other areas were edematous and hyalinized with sparse stromal cells and abundant reticular vessels. Immunohistochemically, the tumor cells reacted strongly and diffusely with antibodies to PAX8, CD10, ?-inhibin, S100 protein, neuron-specific enolase, and vimentin, and they showed focal positivity with antibodies to epithelial membrane antigen and AE1/AE3. Tumor cells were negative for CK7, CK8/18, RCC antigen, synaptophysin, chromogranin, c-kit, D2-40, HMB45, melan-A, cathepsin K, SMA, desmin, CD31, CD34, and estrogen and progesterone receptors. Positive immunoreactivity for PAX8 is unexpected and contrasts to central nervous system (CNS) hemangioblastomas, which are essentially always negative for PAX8. This novel finding adds support to the hypothesis that the immunoprofile of extraneural hemangioblastoma varies with site of origin, perhaps as a result of tumor cell lineage and retention of organ-specific markers or acquisition of site-specific antigens due to local factors.
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Placental methylation markers in normal and trisomy 21 tissues.
Prenat. Diagn.
PUBLISHED: 04-11-2013
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The objective of this study is to combine multiplex ligation-dependent probe amplification (MLPA) and bisulfite sequencing to determine DNA methylation markers for noninvasive prenatal diagnosis of Down syndrome.
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Values of circulating GPC-3 mRNA and alpha-fetoprotein in detecting patients with hepatocellular carcinoma.
HBPD INT
PUBLISHED: 04-06-2013
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The prognosis of hepatocellular carcinoma (HCC) is poor and its early diagnosis is of the utmost importance. This study aimed to investigate the values of glypican-3 (GPC-3) expression in the liver and sera and its gene transcription for diagnosis and monitoring of metastasis of HCC.
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Branching ratio measurements for vacuum ultraviolet photodissociation of 12C16O.
J Phys Chem A
PUBLISHED: 04-02-2013
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The branching ratios for the spin-forbidden photodissociation channels of (12)C(16)O in the vacuum ultraviolet (VUV) photon energy region from 102,500 (12.709 eV) to 106,300 cm(-1) (13.180 eV) have been investigated using the VUV laser time-slice velocity-map imaging photoion technique. The excitations to three (1)?(+) and six (1)? Rydberg-type states, including the progression of W(3s?) (1)?(v = 0, 1, and 2) vibrational levels of CO, have been identified and investigated. The branching ratios for the product channels C((3)P) + O((3)P), C((1)D) + O((3)P), and C((3)P) + O((1)D) of these predissociative states are found to depend on the electronic, vibrational, and rotational states of CO being excited. Rotation and e/f-symmetry dependences of the branching ratios into the spin-forbidden channels have been confirmed for several of the (1)? states, which can be explained using the heterogeneous interaction with the repulsive D(1)?(+) state. The percentage of the photodissociation into the spin-forbidden channels is found to increase with increasing the rotational quantum number for the K(4p?) (1)?(+) (v = 0) state. This has been rationalized using a (1)?(+) to (1)? to (3)? coupling scheme, where the final (3)? state is a repulsive valence state correlating to the spin-forbidden channel.
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A retrospective study of acute pancreatitis in patients with hemorrhagic fever with renal syndrome.
BMC Gastroenterol
PUBLISHED: 03-31-2013
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Etiological diagnosis is an important part of the diagnosis and treatment of acute pancreatitis. Hantavirus infection is a rare cause of acute pancreatitis, which is easy to ignore. There is a need to analyze clinical features of acute pancreatitis caused by Hantavirus.
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Perovskite LaTiO?-Ag0.2 nanomaterials for nonenzymatic glucose sensor with high performance.
Biosens Bioelectron
PUBLISHED: 03-07-2013
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In this paper, a nonenzymatic glucose biosensor based on perovskite LaTiO3-Ag0.2(LTA) modified electrode was presented. The morphology and the composition of the perovskite LaTiO?-Ag0.2 nanomaterials were characterized by using scanning electron microscopy (SEM) and X-ray diffraction (XRD) respectively. The LaTiO?-Ag0.2(LTA) composite was investigated by electrochemical characterization using cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). Under optimal conditions, CV and chronoamperometry (I-t) study revealed that, compared with the bare glassy carbon electrode (GCE), the modified electrode showed a remarkable increase in the efficiency of the electrocatalytic oxidation of glucose, starting at around +0.70 V (vs. Ag/AgCl). The prepared sensor exhibited a high sensitivity of 784.14 µAmM?¹ cm?², a low detection limit of 2.1×10?? M and a wide linear range from 2.5 µM to 4 mM (R=0.9997). More importantly, the LTA modified electrode was also relatively insensitive to commonly interfering species such as ascorbic acid (AA), uric acid (UA), dopamine (DA) in high potential. Moreover, the nonenzymatic sensor was applied to the determination of glucose in human serum samples and the results were in good agreement with clinical data. Electrodes modified with perovskite nanomaterials are highly promising for nonenzymatic electrochemical detection of glucose because of their high sensitivity, fast response, excellent stability and good reproducibility.
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A novel molecular index for secondary oil migration distance.
Sci Rep
PUBLISHED: 02-28-2013
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Determining oil migration distances from source rocks to reservoirs can greatly help in the search for new petroleum accumulations. Concentrations and ratios of polar organic compounds are known to change due to preferential sorption of these compounds in migrating oils onto immobile mineral surfaces. However, these compounds cannot be directly used as proxies for oil migration distances because of the influence of source variability. Here we show that for each source facies, the ratio of the concentration of a select polar organic compound to its initial concentration at a reference point is independent of source variability and correlates solely with migration distance from source rock to reservoir. Case studies serve to demonstrate that this new index provides a valid solution for determining source-reservoir distance and could lead to many applications in fundamental and applied petroleum geoscience studies.
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Acid neutralizing, mechanical and physical properties of pit and fissure sealants containing melt-derived 45S5 bioactive glass.
Dent Mater
PUBLISHED: 02-07-2013
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The aim of this study was to examine the effects of 45S5 bioactive glass (BAG) on the acid neutralizing, mechanical and physical properties of pit and fissure sealants.
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Function of the wheat TaSIP gene in enhancing drought and salt tolerance in transgenic Arabidopsis and rice.
Plant Mol. Biol.
PUBLISHED: 01-14-2013
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Microarray analysis of a salt-tolerant wheat mutant identified a gene of unknown function that was induced by exposure to high levels of salt and subsequently denoted TaSIP (Triticum aestivum salt-induced protein). Quantitative PCR analysis revealed that TaSIP expression was induced not only by salt, but also by drought, abscisic acid (ABA), and other environmental stress factors. Transgenic rice plants that expressed an RNA interference construct specific for a rice gene homologous to TaSIP was more susceptible to salt stress than wild-type rice plants. Subcellular localization studies showed that the TaSIP localized to the cell membrane. Under conditions of salt and drought stress, transgenic Arabidopsis plants that overexpressed TaSIP showed superior physiological properties compared with control plants, including lower Na(+) content and upregulation of several stress resistance genes. Staining of transgenic tissues with ?-glucuronidase (GUS) failed to indicate tissue-specific activity of the full-length TaSIP promoter. Quantitative analysis of GUS fluorescence in transgenic plants treated with ABA or salt stress revealed that the region 1,176-1,410 bp from the start codon contained an ABA-responsive element and that the region 579-1,176 bp from the start codon upstream of the exon contained a salt-stress-responsive element. Based on these results, we conclude that the key part of the TaSIP gene is the region of its promoter involved in salt tolerance.
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Endoscopic resection for gastric epithelial neoplasia: how to solve pathological discrepancy and achieve curative resection?
J Dig Dis
PUBLISHED: 01-10-2013
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Endoscopic resection (ER) was introduced to China from Japan, while most pathologists are influenced by Western criteria. Japanese and Western pathologists have debated for decades how best to distinguish between gastric intraepithelial neoplasia (GIEN) and early gastric cancer (EGC). In this study we aimed to find out how to solve this pathological discrepancy and improve the curative resection of lesions based on our experiences.
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Enhancer of zeste homolog 2 silences microRNA-218 in human pancreatic ductal adenocarcinoma cells by inducing formation of heterochromatin.
Gastroenterology
PUBLISHED: 01-09-2013
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Enhancer of zeste homolog 2 (EZH2) is a histone methyltransferase that is overexpressed by pancreatic ductal adenocarcinoma (PDAC) cells and increases their aggressiveness. We identified microRNAs (miRs) that are regulated by EZH2 and studied their functions in PDAC cells.
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Effects of passive smoking on snoring in preschool children.
J. Pediatr.
PUBLISHED: 01-07-2013
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To examine the association between passive smoking and snoring in preschool children using parent-reported questionnaires and urine cotinine levels.
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[Construction of NK4 gene lentiviral vector and its expression in bone mesenchymal stem cells].
Sheng Wu Yi Xue Gong Cheng Xue Za Zhi
PUBLISHED: 11-22-2011
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The present investigation was to construct lentiviral vector carrying the human gene NK4 and transfect the human bone mesenchymal stem cells (hBMSCs) and to determine the expression of NK4 gene in hBMSCs after transfection. The NK4 gene was obtained from HGF cDNA by polymerase chain reaction(PCR), and the pGC-FU-NK4 plasmid was constructed by double restriction enzyme digestion and gene recombinant. The titer of virus was tested by real-time quantitative PCR. After transfected by lentivirus, the green fluorescent protein (GFP) in hBMSCs was observed using fluorescence microscope, and the expression of NK4 in culture supernatant was detected by enzymelinked immunosorbent assay (ELISA). The sequence of the PCR product was consistent with the data of GeneBank by DNA sequencing. The virus titer was 2 X 10(8)TU/ml. Strong green fluorescence was observed in the cell membrane and cytoplasm of hBMSCs with fluorescent microscopy. The expression of NK4 in culture supernatant was increased with time extension. The hBMSCs can be transfected by NK4 gene expressing lentiviral vector safely and effectively, and the expressin and secretion of NK4 was persistent and stable.
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[Research on Huiji Fenlei Linzheng Fangan-a manuscript in modern times].
Zhonghua Yi Shi Za Zhi
PUBLISHED: 08-17-2011
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Compiled at the end of the 19(th) century and the beginning of the 20(th) century, Fenlei Linzheng Fangan has 48 volumns. FU Si-gong (lived in Nanhui District, Shanghai), the writer, is from a family with generations of doctors. Transcribing medical records of famous doctors and secret books handed down in his family, he devoted decades in compiling the book. This book includes 52 categories according to diseases and symptoms. Every category began with a case report, followed by ancient prescriptions, experiential prescriptions, elaboration on symptoms and pathogenesis, the treatment principle and was combined with an explanation on herbal properties. Working on collating the book has not been finished yet and further work will be needed.
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[Preliminary analysis of chigger mite community on Eothenomys miletus in 19 counties of Yunnan province].
Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi
PUBLISHED: 08-11-2011
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Eothenomys miletus were captured in 19 counties of Yunnan Province. The distribution of species-abundance and the species-plots relationship between E. miletus and chigger mites were analyzed by using ecological statistic method. There were totally 40,052 chigger mites collected from the body surface of 1741 E. miletus. 111 species of chigger mites were identified. The species-abundance distribution showed that with the increase of mite individuals, the number of chigger mite species gradually decreased. Most mite species were rare ones. Species-plot relation indicated that with the number of mouse plots (samples of E. miletus) increasing, the number of chigger mite species increased. E. miletus collected quantity up to date still could not reflect the exact species richness of chigger mite.
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Asymmetric synthesis of tetrahydroquinolines with quaternary stereocenters through the Povarov reaction.
Chemistry
PUBLISHED: 07-28-2011
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The asymmetric Povarov reaction with ?-alkyl styrenes as dienophiles was catalyzed by an N,N-dioxide L4-Sc(OTf)(3) complex. Enantiopure tetrahydroquinoline derivatives with a quaternary stereocenter at the C4 position were synthesized for the first time. A wide variety of ?-alkyl styrenes and N-aryl aldimines were tolerated in the reaction, to give excellent diastereo- (up to 99:1 d.r.) and enantioselectivities (92 to >99% ee). In addition, the reaction could be performed on the gram scale without any loss of yield, diastereoselectivity, or enantioselectivity. An intermolecular hydrogen-shift reaction was found to be a side reaction, which offered a method to synthesize the corresponding quinoline derivatives with chiral quaternary sterocenters.
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[Acupuncture and Tuina in Namibia].
Zhongguo Zhen Jiu
PUBLISHED: 07-12-2011
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The development of acupuncture-moxibustion and Tuina in Namibia was introduced in the article. The history of acupuncture in Namibia is only 15 years, and there are 3 acupuncturists and Tuina practitioners with permanent resident permit in Namibia. The University of Namibia has already established a medical college, which is now cooperating with the concerning Chinese medical university of China and carrying out education for the undergraduate students. The development of acupuncture-moxibustion and Tuina has a great potential in Namibia with an extensive indication involving diseases of internal medicine, surgery, gynecology, pediatrics, orthopedics, and neurology.
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Oncofetal antigen glypican-3 as a promising early diagnostic marker for hepatocellular carcinoma.
HBPD INT
PUBLISHED: 06-15-2011
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Hepatocellular carcinoma (HCC) is characterized by a multi-cause, multi-stage and multi-focus process of tumor progression. Its prognosis is poor and early diagnosis is of utmost importance. This study was undertaken to investigate the dynamic expression of oncofetal antigen glypican-3 (GPC-3) and GPC-3 mRNA in hepatocarcinogenesis and to explore their early diagnostic value for HCC.
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7-Diethyl-amino-2-oxo-2H-chromene-3-carbaldehyde.
Acta Crystallogr Sect E Struct Rep Online
PUBLISHED: 06-08-2011
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In the title compound, C(14)H(15)NO(3), all non-H atoms except for those of the methyl and the disordered ethyl groupare approximately co-planar, the largest deviation from the mean plane being 0.0223?(13)?Å at the N atom. In the crystal, the packing of mol-ecules through weak inter-molecular C-H?O hydrogen-bonding inter-actions leads to the formation of layers parallel to bc plane. Within these layers, there exist slipped ?-? stacking inter-actions between symmetry-related fused rings [centroid-centroid distances = 3.527?(3) and 3.554?(3), slippage = 0.988 and 1.011?Å, respectively]. One ethyl group is disordered over two sets of sites with site-occupation factors of 0.54 and 0.46.
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[Expression features of glypican-3 and its diagnostic and differential values in hepatocellular carcinoma].
Zhonghua Gan Zang Bing Za Zhi
PUBLISHED: 05-19-2011
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To investigate the expression features of glypican-3 (GPC-3) and its diagnostic and differential values in hepatocellular carcinoma (HCC).
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Pharmacokinetics, efficacy, and safety evaluation of docetaxel/hydroxypropyl-sulfobutyl-?-cyclodextrin inclusion complex.
AAPS PharmSciTech
PUBLISHED: 05-05-2011
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Hydroxypropyl-sulfobutyl-?-cyclodextrin (HP-SBE-?-CD) inclusion complex was developed and used as a drug delivery system for DTX (DTX/HP-SBE-?-CD). The objective of the present study was to evaluate and compare the biological properties of DTX/HP-SBE-?-CD with Taxotere®. The pharmacokinetics, biodistribution, antitumor efficacy in vivo and in vitro, and safety evaluation of DTX/HP-SBE-?-CD were studied. The most significant finding was that it was possible to prepare a Polysorbate-80-free inclusion complex for DTX. Studies based on pharmacokinetics, biodistribution, and antitumor efficacy indicated that DTX/HP-SBE-?-CD had similar pharmacokinetic properties and antitumor efficacy both in vitro and in vivo as Taxotere®. Fortunately, this new drug delivery system attenuated the side effects when used in vivo. As a consequence, DTX/HP-SBE-?-CD may be a promising alternative to Taxotere® for cancer chemotherapy treatment with reduced side effects. The therapeutic potential against a variety of human tumors and low toxicity demonstrated in a stringent study clearly warrant clinical investigation of DTX/HP-SBE-?-CD for possible use against human tumors.
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Short amyloid-beta immunogens show strong immunogenicity and avoid stimulating pro-inflammatory pathways in bone marrow-derived dendritic cells from C57BL/6J mice in vitro.
Peptides
PUBLISHED: 04-27-2011
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Amyloid beta peptide 1-15 (A?1-15) and its derivatives have attracted the attention of the scientific community as candidate vaccines for Alzheimers disease (AD) immunotherapy. Recent studies suggested that A?1-42 modulated the immune system by inducing pro-inflammatory dendritic cells (DCs) with reduced antigen-presenting function. However, it remains elusive how A?1-15 impacts DCs function. We therefore investigated the modulation by short A? peptides of DCs from C57Bl/6J mice. Two new immunogens, a tandem repeat of two-lysine-linked A?1-15 sequences with or without an addition of a RGD motif, were tested. Chemotaxis, endocytosis, antigen presenting function and producing cytokines were measured. Both peptides increased migration/endocytosis of immature DCs and MHC II molecule expression/alloreactive T cell activation in TNF-?-matured DCs. In addition, they exhibited decreased production of Th1/Th2 cytokines and pro-inflammatory cytokines. Overall, the two peptides demonstrated strong immunogenicity but did not stimulate pro-inflammatory pathways. These results support the use of short A? immunogens in AD immunotherapy.
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What is Visualize?

JoVE Visualize is a tool created to match the last 5 years of PubMed publications to methods in JoVE's video library.

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We use abstracts found on PubMed and match them to JoVE videos to create a list of 10 to 30 related methods videos.

Video X seems to be unrelated to Abstract Y...

In developing our video relationships, we compare around 5 million PubMed articles to our library of over 4,500 methods videos. In some cases the language used in the PubMed abstracts makes matching that content to a JoVE video difficult. In other cases, there happens not to be any content in our video library that is relevant to the topic of a given abstract. In these cases, our algorithms are trying their best to display videos with relevant content, which can sometimes result in matched videos with only a slight relation.