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Find video protocols related to scientific articles indexed in Pubmed.
[Clinical characteristics and prognosis comparison of Chinese non ST-segment elevation acute coronary syndrome patients in two different time periods].
Zhonghua Xin Xue Guan Bing Za Zhi
PUBLISHED: 11-13-2014
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To compare the clinical characteristics, treatment methods and outcomes in Chinese non ST-segment elevation acute coronary syndrome (NSTE-ACS) patients from two large clinical trials in different time periods.
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Bartonella spp. in a Puerto Rican Bat Community.
J. Wildl. Dis.
PUBLISHED: 11-08-2014
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Abstract We captured and sampled 68 bats of six species from a shared roosting site in Puerto Rico in April 2012. Bats were screened for Bartonella spp. by culture and confirmed by PCR and sequencing for the gltA gene. Bartonella cultures were obtained from blood specimens of 9/51 (18%) individuals from three species (Artibeus jamaicensis, Brachyphylla cavernarum, and Monophyllus redmani). Phylogenetic analysis of the gltA sequences showed that M. redmani was infected with multiple, diverse Bartonella strains, and A. jamaicensis was infected with a strain related to a strain from a congeneric host. Ectoparasite load could possibly explain observed differences in Bartonella diversity and prevalence between bat species in this community, and we suggest future research to substantiate these preliminary findings.
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Select the optimized effective dose to reduce nuclear radiations in pediatric nuclear medicine.
World J Nucl Med
PUBLISHED: 09-06-2014
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Many techniques and research models on calculating and reducing the nuclear radiation dose on pediatric nuclear medicine procedure have been developed and reported in recent years. However, most those models either utilized simple shapes to present the organs or used more realistic models to estimate the nuclear dose applied on pediatric patients. The former are too simple to provide accurate estimation results, and the latter are too complicated to intensively involve complex calculations. In this study, a simple but practical model is developed to enable physicians to easily and quickly calculate and select the average optimal effective nuclear dose for the given age and body-size of the pediatric patients. This model is built based on one research result reported by Frederic Fahey, et al and it can be easily implemented in most common pediatric nuclear medicine procedures. This is the first research of using fuzzy inference system to calculate the optimal effective dose applied in the nuclear medicine for pediatric patients.
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Small Bowel Endoscopy Diagnostic Yield and Reasons of Obscure GI Bleeding in Chinese Patients.
Gastroenterol Res Pract
PUBLISHED: 08-11-2014
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Aim. To investigate the diagnostic yield and etiologies of patients with obscure gastrointestinal bleeding (OGIB) using capsule endoscopy (CE) or double-balloon enteroscopy (DBE). Method. We studied the data of 532 consecutive patients with OGIB that were referred to Xinqiao Hospital in Chongqing from December 2005 to January 2012. A lesion that was believed to be the source of the bleeding (ulceration, mass lesion, vascular lesion, visible blood, inflammation, or others) was considered to be a positive finding. We analyzed the diagnostic yield of CE and SBE and the etiologies of OGIB. Result. CE and SBE have similar diagnostic yields, at 71.9% (196/231) and 71.8% (251/304), respectively. The most common etiology was erosions/ulceration (27.1%) followed by mass lesion (19.4%) and angiodysplastic/vascular lesions (13.9%). By stratified analysis, we found that erosions/ulceration (27.1%) was the most common etiology for the 21-40-year age group. Mass lesion was the most common etiology in the 41-60-year age group. However, in the >60 years age group, angiodysplastic/vascular lesions were significantly increased compared with the other groups, even though erosions/ulceration was most common. Conclusion. In this study, we found that CE and SBE have similar diagnostic yields and erosions/ulceration was the most common reason for OGIB, followed by mass lesion and angiodysplasias.
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Endoscopic submucosal dissection for superficial esophageal cancer with near-circumferential lesions: our experience with 40 patients.
Surg Endosc
PUBLISHED: 08-02-2014
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Nowadays, there are few reports indicating whether early esophageal cancers (EsC) with near-circumferential lesions are still appropriate for the endoscopic submucosal dissection (ESD) procedure.
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Synthesis of gold nanorods and their functionalization with bovine serum albumin for optical hyperthermia.
J Biomed Nanotechnol
PUBLISHED: 07-15-2014
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Although gold nanorods (GNRs) have been investigated extensively for optical hyperthermia therapies, the synthesis of rods is far from ideal. In this report, we optimized the synthesis of gold nanorods using hydroquinone as a reducing agent. Compared with the GNRs prepared by traditional ways, the as-synthesized rods have a flexibly tunable size and wider range of longitudinal surface plasmon resonance (LSPR). Furthermore, a series of small-length gold nanorods with length ranging from 30 to 90 nm were synthesized and they are more suitable for in vivo biomedical applications. Finally, we exploited a convenient approach for preparing water-soluble GNRs with less toxicity, better dispersion and flexible functionalization by exchanging hexadecyltrimethylammonium bromide (CTAB) on the surface of the rods with carboxylated bovine serum albumin (BSA) derivative, the BSA modified GNRs showed significant anticancer efficacy through near infrared (NIR) hyperthermia. We believe that the as-prepared gold nanorods will find promising applications in biomedical fields, especially in cancer therapy.
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Endoscopic treatment of delayed colon perforation: the enteroscopy overtube approach.
Endoscopy
PUBLISHED: 04-28-2014
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Delayed perforation is a rare complication of therapeutic colonoscopy, and it is severe and sometimes lethal. This paper reports on a new minimally invasive method for the treatment of delayed colonic perforation.
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Frictional auto-roughening of a surface with spatially varying stiffness.
Soft Matter
PUBLISHED: 03-22-2014
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We show that significant reduction of sliding friction can be achieved between a rigid surface and a flat elastic surface by spatial variation in stiffness of the latter. This reduction in friction during sliding occurs due to an "auto-roughening" phenomenon in which a fully connected contact region breaks into partial contact. An elastomer, poly(dimethylsiloxane) (PDMS), was used to fabricate nominally flat surfaces with regions of two different stiffness, achieved by using two different concentrations of the cross-linker. Both experiments and finite element simulation show that, for sufficiently high friction and low normal load, the real contact area between a rigid indenter and a surface with spatially varying stiffness is reduced significantly due to auto-roughening. The finite element model also shows how the auto-roughening depends on the properties and geometry of interfacial structures, resulting in reduced overall friction.
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Multimodal nanoprobes evaluating physiological pore size of brain vasculatures in ischemic stroke models.
Adv Healthc Mater
PUBLISHED: 03-21-2014
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Ischemic stroke accounts for 80% strokes and originates from a reduction of cerebral blood flow (CBF) after vascular occlusion. For treatment, the first action is to restore CBF by thrombolytic agent recombinant tissue-type plasminogen activator (rt-PA). Although rt-PA benefits clinical outcome, its application is limited by short therapeutic time window and risk of brain hemorrhage. Different to thrombolytic agents, neuroprotectants reduce neurological injuries by blocking ischemic cascade events such as excitotoxicity and oxidative stress. Nano-neuroprotectants demonstrate higher therapeutic effect than small molecular analogues due to their prolonged circulation lifetime and disrupted blood-brain barrier (BBB) in ischemic region. Even enhanced BBB permeability in ischemic territories is verified, the pore size of ischemic vasculatures determining how large and how efficient the therapeutics can pass is barely studied. In this work, nanoprobes (NPs) with different diameters are developed. In vivo multimodal imaging indicates that NP uptakes in ischemic region depended on their diameters and the pore size upper limit of ischemic vasculatures is determined as 10-11 nm. Additionally, penumbra defined as salvageable ischemic tissues performed a higher BBB permeability than infarct core. This work provides a guideline for developing nano-neuroprotectants by taking advantage of the locally enhanced BBB permeability in ischemic brain tissues.
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Analysis and pharmacokinetic study of polyphyllin H in beagle dog plasma after oral administration of Rhizoma Paridis extracts by LC-MS/MS.
Biomed. Chromatogr.
PUBLISHED: 03-14-2014
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A highly sensitive, rapid assay method has been developed and validated for the analysis of polyphyllin H in beagle dog plasma with liquid chromatography coupled to tandem mass spectrometry with electrospray ionization in the positive-ion mode. The assay procedure involves extraction of polyphyllin H and ginsenoside Re (IS) from beagle dog plasma. Chromatographic separation was carried out on an Agilent Zorbax XDB-C18 (100?×?2.1 mm, 1.8?m) column by isocratic elution with acetonitrile and water (50:50, v/v) at a flow rate of 0.25 mL/min with a total run time of 2.5 min. The MS/MS ion transitions monitored were 870.46???869.6 for polyphyllin H and 947.12???969.60 for IS. Linear responses were obtained for polyphyllin H ranging from 1 to 50 ng/mL. The intra-and inter-day precisions (RSDs) were <1.77 and 3.39% and the extraction recovery ranged from 91.89 to 93.33% with RSD <2.68%. Stability studies showed that polyphyllin H was stable in the preparation and analytical process. The results indicated that the validated method was successfully used to determine the concentration-time profiles of polyphyllin H. Copyright © 2014 John Wiley & Sons, Ltd.
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Synthesis, structure, fluorescence spectra study of two kinds of coordination supramolecular zinc compounds.
Spectrochim Acta A Mol Biomol Spectrosc
PUBLISHED: 01-26-2014
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Two novel compounds [Zn(IAA)2(phen) (HIAA=indole-3-acetic acid, phen=1,10-phenanthroline) (1) and [Zn(IAA)2(4,4'-bipy)](4,4'-bipy=4,4'-bipyridine) (2) were synthesized by the reaction of Zn(Ac)2·2H2O or Zn(SO4)·4H2O as a metal source, HIAA as the first ligand and phen or 4,4'-bipy as the second ligand in the system of methanol or the mixed solution of methanol and water at room temperature. They were characterized by elemental analysis, IR spectroscopy, UV-vis spectra and single-crystal X-ray diffraction. Structural analysis shows that the center metal Zn(II) for the compound 1 is four-coordinated, displaying a distorted tetrahedron; the metal center coordinated model for compound 2 is similar to that of 1, in which the structural unit of Zn(IAA)2 was connected by bridging the 4, 4'- bipy ligand to form an infinite 1D chain. In the packing of the compounds, there are some hydrogen bonding interactions and by the hydrogen bonding, 1D and 2D supramolecular structures were formed. Additional, we have studied the fluorescent properties of the two compounds.
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IL-17A Induces MIP-1? Expression in Primary Astrocytes via Src/MAPK/PI3K/NF-kB Pathways: Implications for Multiple Sclerosis.
J Neuroimmune Pharmacol
PUBLISHED: 01-25-2014
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Neuroinflammation plays critical roles in multiple sclerosis (MS). In addition to the part played by the lymphocytes, the underlying mechanisms could, in part, be also attributed to activation mediated by astrocytes. Macrophage inflammatory protein-1? (MIP-1?) has been implicated in a number of pathological conditions, specifically attributable to its potent chemottractant effects. Its modulation by IL-17, however, has received very little attention. In the present study, we demonstrated IL-17-mediated induction of MIP-1? in rat primary astroctyes through its binding to the cognate IL-17RA. Furthermore, this effect was mediated via the activation of Src, mitogen-activated protein kinases (MAPKs), PI3K/Akt and NF-kB pathways, culminating ultimately into increased expression of MIP-1?. Exposure of primary mouse astrocytes to IL-17 resulted in increased expression of glial fibrillary acidic protein and, this effect was abrogated in cells cultured in presence of the MIP-1? neutralizing antibody, thus underscoring its role in the activation of astrocytes. In vivo relevance of these findings was further corroborated in experimental autoimmune encephalomyelitis mice that demonstrated significantly increased activation of astrocytes with concomitant increased expression of MIP-1? in the corpus callosum compared with control group. Understanding the regulation of MIP-1? expression may provide insights into the development of potential therapeutic targets for neuroinflammation associated with multiple sclerosis.
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Effect of different drying methods on the quality of Angelicae Sinensis Radix evaluated through simultaneously determining four types of major bioactive components by high performance liquid chromatography photodiode array detector and ultra-high performance liquid chro
J Pharm Biomed Anal
PUBLISHED: 01-20-2014
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In the present study, the effect of drying methods on the quality of Angelicae Sinensis Radix (DG), was evaluated by newly developed high performance liquid chromatography photodiode array detector (HPLC-DAD) and ultra-high performance liquid chromatography quadrupole time-of-flight mass spectrometry (UHPLC-QTOF-MS/MS). Ten major bioactive components including two phenolic acids, two hydroxyl phthalides, four alkyl phthalides and two phthalide dimers were selected as evaluation chemical markers and the newly-established method was qualitatively and quantitatively validated. DG slices and whole roots dried in shade, sun light, hot air, vacuum, microwave, far infrared ray and combination of microwave and far infrared ray as well as the fresh DG samples were determined by the established methods. DG slices dried in hot air kept the similar chemical composition to that of fresh DG, while DG whole roots dried in vacuum retained highest contents of the major components. Coniferyl ferulate and ligustilide degraded significantly in DG slices dried by microwave, far infrared ray and their combination. The influence of such chemical changes induced by different drying methods on the bioactivities of DG warrants further investigation, so that the optimal drying method can be obtained for the standardization of DG herb.
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A comprehensive microRNA expression profile of the backfat tissue from castrated and intact full-sib pair male pigs.
BMC Genomics
PUBLISHED: 01-17-2014
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It is widely known that castration has a significant effect on the accumulation of adipose tissue. microRNAs (miRNAs) are known to be involved in fat deposition and to be regulated by the androgen-induced androgen receptor (AR). However, there is little understanding of the relationship between miRNAs and fat deposition after castration. In this study, the high-throughput SOLiD sequencing approach was used to identify and characterize miRNA expression in backfat from intact and castrated full-sib male 23-week-old pigs. The patterns of adipogenesis and fat deposition were compared between castrated and intact male pigs.
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Synthesis, crystal structure, photoluminescence property and photoelectronic behavior of two uranyl-organic frameworks constructed from 1, 2, 4, 5-benzenetetracarboxylic acid as ligand.
Spectrochim Acta A Mol Biomol Spectrosc
PUBLISHED: 01-09-2014
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By using 1, 2, 4, 5-benzenetetracarboxylic acid as organic ligands, two uranyl coordination complexes, (UO2)2(bta)(DMA)2 (1) and [(UO2)2(bta)(?3-OH2)]·2[HN(CH3)2]·H2O (2) (H4bta=1, 2, 4, 5-benzenetetracarboxylic acid, DMA=N,N-Dimethylacetamide) were synthesized. The X-ray single crystal analysis revealed that complex 1 exhibits a 3-demensional framework, while complex 2 exhibits a 2-demensional framework. In order to furthermore characterize the two complexes and extend their functional properties, spectroscopies of IR, UV-vis, photoluminescence and surface photovoltage were also studied primarily. In addition, thermogravimetric analyses and photocatalytic studies for complexes 1 and 2 were discussed in detail.
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The expression of ?-catenin in esophageal squamous cell carcinoma and its correlations with prognosis of patients.
Hum. Pathol.
PUBLISHED: 01-07-2014
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As a member of the catenin family, expression of ?-catenin and its clinical implication in numerous tumors remain unclear. In the present study, expression of ?-catenin in esophageal squamous cell carcinoma (ESCC) and its correlations with patient prognosis were explored. We detected the expression of ?-catenin, by immunohistochemistry, in ESCC tissues from 299 cases and analyzed the correlation between ?-catenin expression and patient clinicopathological features. Compared with a lack of expression in adjacent normal esophageal epithelium (0%, 0/47), the frequency of ?-catenin protein was increased in ESCC tissues to 41.5% (124/299, P < .001) and expression correlated with TNM stage and lymph node metastasis (P = .025 and .019, respectively). Furthermore, Kaplan-Meier survival analysis revealed that patients with high ?-catenin expression had shorter survival than patients with low expression (P = .010), and multivariate Cox analysis revealed that high ?-catenin expression was also an independent prognostic factor (P = .001). In transwell assays, migration of ESCC cells was enhanced by ?-catenin overexpression, whereas proliferation of ESCC cells was unchanged. Together, our results suggest that ?-catenin acts as an oncoprotein when overexpressed in ESCC, and its expression is associated with poor prognosis and malignant cell behavior.
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Regulation of CARD8 expression by ANRIL and association of CARD8 single nucleotide polymorphism rs2043211 (p.C10X) with ischemic stroke.
Stroke
PUBLISHED: 01-02-2014
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ANRIL has long been considered as the strongest candidate gene at the 9p21 locus, robustly associated with stroke and coronary artery disease. However, the underlying molecular mechanism remains unknown. The present study works to elucidate such a mechanism.
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The relationship between frontal lobe lesions, course of post-stroke depression, and 1-year prognosis in patients with first-ever ischemic stroke.
PLoS ONE
PUBLISHED: 01-01-2014
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Most studies on post-stroke depression (PSD) have focused on a certain time point after stroke instead of the time course of PSD. The aim of this study was to determine the relationship between frontal lobe lesions, course of PSD over a year following the stroke onset, and the 1-year prognosis in patients with first-ever ischemic stroke.
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Image-guided pro-angiogenic therapy in diabetic stroke mouse models using a multi-modal nanoprobe.
Theranostics
PUBLISHED: 01-01-2014
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The efficacy of pro-angiogenic therapy is difficult to evaluate with current diagnostic modalities. The objectives were to develop a non-invasive imaging strategy to define the temporal characteristics of angiogenesis and to evaluate the response to pro-angiogenic therapy in diabetic stroke mouse models.
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Renal Lipids and Oxygenation in Diabetic Mice: Noninvasive Quantification with MR Imaging.
Radiology
PUBLISHED: 10-28-2013
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Purpose To determine the relationship between renal lipid content and intrarenal oxygenation in diabetic nephropathy by using noninvasive chemical shift-selective (CSS) imaging and blood oxygen level-dependent (BOLD) magnetic resonance (MR) imaging. Materials and Methods The study was approved by the institutional Committee on Animal Research. Lipid and water phantoms for CSS imaging were made, and BOLD MR imaging phantoms from arterial and venous blood samples were collected from rats. CSS imaging and BOLD imaging were performed to measure lipid contents and T2* in phantoms and kidneys of diabetic gene (db) db/db mice and wild-type mice after exposure to nitrogen (four per group) and injection of furosemide (four per group). Results of MR imaging-measured lipid contents and oxygen tension were compared with known values in phantoms and reference standard from mice with histologic data. Statistical analysis was performed with independent sample and paired sample t tests and Pearson correlation test. Results Renal lipid content in db/db mice was significantly higher compared with that in control mice (9.40% ± 1.89 and 3.11% ± 0.57, respectively; P < .001). In addition, the lipid content in the cortex of db/db mice was significantly higher than that in medulla (12.73% ± 0.94 and 3.16% ± 0.50, respectively; P < .001). Correlation was significant between T2* measured with BOLD and oxygen tension in blood phantoms (r = 0.958; P < .001). Lower baseline T2* in diabetic kidney suggested lower oxygenation that reserved excess oxygen supply. Lower oxygenation in diabetic kidney cortex was observed after nitrogen exposure and furosemide injection. Conclusion Noninvasive CSS imaging and MR imaging of db/db diabetic mice revealed the relationship between the renal lipid content and intrarenal oxygenation in diabetic kidney. Lipid accumulation in diabetic kidney compromises the oxygenation of the renal tissue and made it more susceptible to renal hypoxia. © RSNA, 2013 Online supplemental material is available for this article.
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[Feasibility and outcome of rotational atherectomy for treating resistant chronic total occlusions].
Zhonghua Xin Xue Guan Bing Za Zhi
PUBLISHED: 10-12-2013
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To assess the application of rotational atherectomy to improving the success rate and outcome of percutaneous recanalization of resistant chronic total occlusion (CTO), i.e. the guidewire could cross the lesion but it is impossible to advance any device over the wire through the occluded segment.
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Effects of low molecular weight heparin on clot rate and activated clotting time: an in vitro study.
Chin. Med. J.
PUBLISHED: 09-17-2013
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Due to lack of point-of-care testing, the use of low-molecular-weight heparin (LMWH) therapy in some special patients is restricted. This study was designed to explore the effects of LMWH on clot rate (CR) and activated clotting time (ACT), and to search for an appropriate method for bedside monitoring of anticoagulant activity of LMWH.
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[A research on the construction of an assessment index system to evaluate the implementation of health promotion in enterprises].
Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi
PUBLISHED: 08-03-2013
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To establish an assessment index system to objectively evaluate the implementation of health promotion in enterprises.
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The IL-33-ST2L pathway is associated with coronary artery disease in a Chinese Han population.
Am. J. Hum. Genet.
PUBLISHED: 06-27-2013
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The effects of interleukin-33 (IL-33) on the immune system have been clearly demonstrated; however, in cardiovascular diseases, especially in coronary artery disease (CAD), these effects have not yet been clarified. In this study, we investigate the genetic role of the IL-33-ST2L pathway in CAD. We performed three-stage case-control association analyses on a total of 4,521 individuals with CAD and 4,809 controls via tag SNPs in the genes encoding IL-33 and ST2L-IL-1RL1. One tag SNP in each gene was significantly associated with CAD (rs7025417(T) in IL33, padj = 1.19 × 10(-28), OR = 1.39, 95% CI: 1.31-1.47; rs11685424(G) in IL1RL1, padj = 6.93 × 10(-30), OR = 1.40, 95% CI: 1.32-1.48). Combining significant variants in two genes, the risk for CAD increased nearly 5-fold (padj = 8.90 × 10(-21), OR = 4.98, 95% CI: 3.56-6.97). Traditional risk factors for CAD were adjusted for the association studies by SPSS with logistic regression analysis. With the two variants above, both located within the gene promoter regions, reporter gene analysis indicated that the rs7025417 C>T and rs11685424 A>G changes resulted in altered regulation of IL33 and IL1RL1 gene expression, respectively (p < 0.005). Further studies revealed that the rs7025417 genotype was significantly associated with plasma IL-33 levels in the detectable subjects (n = 227, R(2) = 0.276, p = 1.77 × 10(-17)): the level of IL-33 protein increased with the number of rs7025417 risk (T) alleles. Based on genetic evidence in humans, the IL-33-ST2L pathway appears to have a causal role in the development of CAD, highlighting this pathway as a valuable target for the prevention and treatment of CAD.
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A high performance liquid chromatography fingerprinting and ultra high performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry chemical profiling approach to rapidly find characteristic chemical markers for quality evalu
J Pharm Biomed Anal
PUBLISHED: 06-11-2013
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A high performance liquid chromatography-photodiode array detector (HPLC-PDA) fingerprinting and ultra high performance liquid chromatography-photodiode array detector coupled with quadrupole time-of-flight mass spectrometry (UHPLC-PDA-QTOF-MS/MS) based chemical profiling approach was developed to rapidly find characteristic chemical markers for quality control of dispensing granules, taking Chuanxiong Rhizoma (CR) as a model herb. Firstly, CR crude drugs, their traditional decoctions and CR dispensing granules were analyzed by HPLC-PDA to rapidly establish the fingerprints and thereby generate the simulative median chromatograms of CR crude drugs, decoctions and dispensing granules, and by comparing the simulative median chromatograms, major characteristic peaks of CR decoctions and dispensing granules could be determined. Secondary, UHPLC-PDA-QTOF-MS/MS was used to identify the major characteristic peaks of CR decoctions and dispensing granules. The identities of three major peaks were elucidated and confirmed to be ferulic acid (1), senkyunolide I (2) and senkyunolide H (3) by comparing the mass/UV spectra and retention times with that of the reference compounds. Thirdly, an HPLC-PDA method was validated to quantify the three characteristic components in commercial CR dispensing granules. The average contents of ferulic acid and senkyunolide H were found to be less than 1.0mg/g, whereas that of senkyunolide I was 4.40mg/g in CR dispensing granules, which indicated that senkyunolide I might be chosen as a suitable quantitative marker, while ferulic acid and senkyunolide H as qualitative markers for the quality evaluation of CR dispensing granules. It is suggested that this newly established approach could be used to practically and rapidly find suitable marker compounds for quality control of dispensing granules derived from other medicinal herbs.
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Asymmetric zinc-catalyzed hydrosilylation of ketones and the effect of carboxylate on the enantioselectivity.
Chirality
PUBLISHED: 04-27-2013
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Several chiral ligands containing (R,R)-diaminocyclohexane moieties and pyrrole, furan, or benzene have been synthesized. These ligands were tested in enantioselective zinc-catalyzed hydrosilylation reactions; excellent enantioselectivities were obtained when the ligands containing (R,R)-diaminocyclohexane moieties and furan rings were used. For comparison, zinc chloride combined with different potassium carboxylate salts and ligands were also tested for catalytic hydrosilylation reactions.
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Synthesis, structures and properties of the catalytic bromination reaction of a series of novel scorperate oxidovanadium complexes with the potential detection of hydrogen peroxide in water.
Spectrochim Acta A Mol Biomol Spectrosc
PUBLISHED: 03-28-2013
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A series of scorpionate oxovanadium (IV) complexes: [VO(Tp(4I))(pz)(SCN)]·1/2CH2Cl2 (1), [VO(Tp)(pzTp)]·2H2O (2), [VO(Bp)(Tp(4I))] (3) and [VO(C5H7O2)(Tp(4I))]·CH3OH (4) (Bp: [H2B(pz)(2-)], Tp: [HB(pz)(3-)], Tp(4I): [HB(4I-pz)(3-)], pzTp: [B(pz)(4-)]) have been synthesized and characterized by elemental analysis, IR spectra, UV-Vis spectroscopy, powder X-ray diffraction, single-crystal X-ray diffraction and thermal gravimetric analysis (TG). Structural analysis shows that the coordination environment of vanadium atom is N5O, to form a distorted octahedron geometry. In addition, the catalytic activities of the bromination reactions for complexes 1 and 2 in phosphate buffer with phenol red as a trap were evaluated primary by UV/Vis spectroscopy, and a practical application of H2O2 detection was firstly observed in the catalytic reaction system.
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Suppression of experimental abdominal aortic aneurysms in the mice by treatment with Ginkgo biloba extract (EGb 761).
J Ethnopharmacol
PUBLISHED: 03-27-2013
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Ginkgo biloba extract (EGb 761) is widely used to treat cerebral disorders. Clinical trials have demonstrated therapeutic benefits of EGb 761 in various vascular diseases. Because the potential pathophysiological mechanisms appear similar to those involved in aneurysmal degeneration, we postulated that EGb 761 might affect the development and progression of experimental abdominal aortic aneurysm (AAA). This study was aimed to investigate whether EGb 761 influences the development of experimental AAAs, and to explore the underlying mechanisms.
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Bartonella bovis isolated from a cow with endocarditis.
J. Vet. Diagn. Invest.
PUBLISHED: 03-21-2013
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A 7-year-old pregnant Angus cow was found dead in the field. At necropsy, the aortic valve was expanded by moderate fibrous connective tissue and acidophilic coagulum containing multifocal marked bacteria, mineral, neutrophils, and red blood cells. Numerous tiny grayish, opaque bacterial colonies were detected on blood agar plates at 7 days after inoculation with a swab of the heart valve of the cow. The bacterium was a Gram-negative, very small coccobacillus that was catalase, oxidase, and urease negative, and did not change litmus milk, triple sugar iron agar, and sulfide-indole-motility medium. The bacterium was negative for esculin hydrolysis, phenylalanine deaminase, nitrate reduction, and gelatin hydrolysis. The isolate did not produce acid from glycerol, inulin, lactose, maltose, mannose, raffinose, salicin, sorbitol, sucrose, trehalose, glycogen, ribose, or starch. Polymerase chain reaction tests for the gltA, ssrA, ftsZ, ribC, rpoB, and 16S ribosomal RNA genes of Bartonella species were positive for the isolate. Amplicons were sequenced, and the gltA, ribC, ssrA, and 16S ribosomal RNA gene sequences were found to have 100% homology to the type strain of Bartonella bovis, whereas the fts and rpoB sequences showed 99.9% and 99.6% homology, respectively, to the type strain of Bartonella bovis. Diagnosticians should be aware of slow-growing microorganisms, and culture media should be incubated beyond the standard period to enhance the recovery of Bartonella species.
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Effective DNA epitope chimeric vaccines for Alzheimers disease using a toxin-derived carrier protein as a molecular adjuvant.
Clin. Immunol.
PUBLISHED: 02-22-2013
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Active amyloid-beta (A?) immunotherapy is under investigation to prevent or treat Alzheimer disease (AD). We describe here the immunological characterization and protective effect of DNA epitope chimeric vaccines using 6 copies of A?1-15 fused with PADRE or toxin-derived carriers. These naked 6A?15-T-Hc chimeric DNA vaccines were demonstrated to induce robust anti-A? antibodies that could recognize A? oligomers and inhibit A? oligomer-mediated neurotoxicity, result in the reduction of cerebral A? load and A? oligomers, and improve cognitive function in AD mice, but did not stimulate A?-specific T cell responses. Notably, toxin-derived carriers as molecular adjuvants were able to substantially promote immune responses, overcome A?-associated hypo-responsiveness, and elicit long-term A?-specific antibody response in 6A?15-T-Hc-immunized AD mice. These findings suggest that our 6A?15-T-Hc DNA chimeric vaccines can be used as a safe and effective strategy for AD immunotherapy, and toxin-derived carrier proteins are effective molecular adjuvants of DNA epitope vaccines for Alzheimers disease.
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Protective effects of urinary trypsin inhibitor on vascular permeability following subarachnoid hemorrhage in a rat model.
CNS Neurosci Ther
PUBLISHED: 01-31-2013
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Inflammation and apoptosis play important roles in increasing vascular permeability following subarachnoid hemorrhage (SAH). The objective of this study was to evaluate whether urinary trypsin inhibitor (UTI), a serine protease inhibitor, attenuates vascular permeability by its antiinflammatory and antiapoptotic effects after experimental SAH.
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A comparative mutational analysis of HIV-1 Vpu subtypes B and C for the identification of determinants required to counteract BST-2/Tetherin and enhance viral egress.
Virology
PUBLISHED: 01-31-2013
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We have undertaken a genetic strategy to map Vpu regions necessary for BST-2 antagonism and viral egress. This approach is based on our identification of an egress-defective Vpu variant encoded by an HIV-1 subtype C strain. We constructed a series of chimeric Vpu molecules made from the Vpu C variant and Vpu B from a standard laboratory strain. The TM domain from the inactive Vpu C, which contains multiple non-conserved residues, was responsible for a significant decrease in egress activity and BST-2 downregulation, confirming the functional importance of the Vpu TM domain. However, for complete inactivation, both the N-terminus and TM domain from the inactive Vpu C molecule were required, suggesting a new role for the Vpu N-terminus. In addition, determinants in the C-terminus of Vpu B that may be involved in efficient TGN accumulation were also necessary for enhanced viral egress but are missing or non-functional in Vpu C.
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Transmission and maintenance cycle of Bartonella quintana among rhesus macaques, China.
Emerging Infect. Dis.
PUBLISHED: 01-26-2013
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We detected Bartonella quintana in 48.6% of captive rhesus macaques from an animal facility in Beijing, China. Prevalence of infection increased over the period of observation. Our findings suggest that macaques may serve as reservoir hosts for B. quintana and that Pedicinus obtusus lice might act as efficient vectors.
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Diepitope multiple antigen peptide of hTERT trigger stronger anti-tumor immune responses in vitro.
Int. Immunopharmacol.
PUBLISHED: 01-25-2013
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Rapid advances in immune system knowledge have led to the exploration of immunologic approaches for eliminating tumor cells. Human telomerase reverse transcriptase (hTERT) is considered to be an ideal universal target for novel immunotherapies against cancers. Thus far, studies of effective antitumor immunotherapies have focused on the quantity and quality of the effector function of the CD8 compartment. However, increasing evidence has demonstrated that CD4+ T cells play important roles in generating and maintaining antitumor immune responses in animal models. The aim of this work was to verify whether diepitope multiple antigen peptides (MAPs) that were composed of the cytotoxic T lymphocyte (CTL) epitope of hTERT and the T-helper epitope of hTERT could improve upon the immunogenicity of a monoepitope MAP of hTERT. Dendritic cells (DCs) pulsed with diepitope MAPs composed of the CTL epitope hTERT-540 and the T-helper epitope hTERT-766 were used to evaluate immune responses against various tumor cells. A standard in vitro 4-h ?¹Cr-release assay was employed in this study. The results demonstrated that CTLs activated by the diepitope MAP that consisted of hTERT-540 and hTERT-766 could cause 8.56% more lysis than CTLs activated by the monoepitope MAP containing hTERT-540. Moreover, the activated CTLs could kill neither hTERT-negative tumor cells, such as U2OS cells, nor HLA-A2 negative cells, such as HepG2 cells. Our results indicate that diepitope MAPs that are generated from hTERT can be exploited for cancer immunotherapy.
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Overexpression of DICER1 induced by the upregulation of GATA1 contributes to the proliferation and apoptosis of leukemia cells.
Int. J. Oncol.
PUBLISHED: 01-17-2013
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Dicer, a member of the RNase III family, is the key enzyme required for the biogenesis of microRNAs and small interfering RNAs. Recent evidence indicates that DICER1 expression levels vary among different solid tumors and decreased or increased DICER1 expression has been associated with aggressive cancers. In this study, we assessed DICER1 expression levels in acute myeloid leukemia (AML) and investigated its biological effects and transcriptional regulation in leukemia cell lines. We demonstrated that DICER1 was overexpressed in AML patients and leukemia cell lines by real-time quantitative PCR and western blot analysis. A functional assay demonstrated that the silencing of DICER1 inhibited cell proliferation and promoted apoptosis in leukemia cell lines. We also demonstrated that DICER1 was upregulated by the hematopoietic transcription factor, GATA1, through luciferase, electrophoretic mobility shift and chromatin immunoprecipitation assays. These data suggest that DICER1 plays an important role in AML and the finding that the upregulation of DICER1 is induced by GATA1 may provide a framework for the understanding of differential DICER1 expression levels in multiple types of cancer.
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Genetic diversity of Bartonella quintana in macaques suggests zoonotic origin of trench fever.
Mol. Ecol.
PUBLISHED: 01-13-2013
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Bartonella quintana is a bacterium that causes a broad spectrum of diseases in humans including trench fever. Humans were previously considered to be the primary, if not the only, reservoir hosts for B. quintana. To identify the animal reservoir and extend our understanding of the ecological and evolutionary history of B. quintana, we examined blood samples from macaques and performed multilocus sequence typing (MLST) analysis. We demonstrated the prevalence of B. quintana infection was common in macaques from main primate centres in mainland China. Overall, 18.0% (59/328) of rhesus macaques and 12.7% (39/308) of cynomolgus macaques were found to be infected with B. quintana by blood culture and/or polymerase chain reaction. The infection was more frequently identified in juvenile and young monkeys compared with adult animals. In contrast with the relatively low level of sequence divergence of B. quintana reported in humans, our investigation revealed much higher genetic diversity in nonhuman primates. We identified 44 new nucleotide variable sites and 14 novel sequence types (STs) among the B. quintana isolates by MLST analysis. Some STs were found only in cynomolgus macaques, while some others were detected only in rhesus macaques, suggesting evidence of host-cospeciation, which were further confirmed by phylogenetic analysis and Splits decomposition analysis. Our findings suggest that trench fever may primarily be a zoonotic disease with macaques as the natural hosts.
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Role of the CPC sequence in the antioxidant activity of GcGAST protein in E.coli.
Protein J.
PUBLISHED: 01-10-2013
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Gibberellic acid stimulated transcriptional protein from Gymnadenia conopsea (GcGAST) is a novel member of GA-induced cysteine-rich protein family, which shared 12 highly conserved cysteine residues with other members in C-terminal domain. In the present paper, the recombinant plasmid, as well as two mutants Serine-Proline-Cysteine (SPC) and Cysteine-Proline-Serine (CPS), were constructed to investigate for the first time the effects of the cysteines in Cysteine-Proline-Cysteine (CPC) sequence on the antioxidant activity of GcGAST protein. It was found that E.coli expressing wt GcGAST exhibited significant resistance against exogenous H(2)O(2). Similar phenomenon was observed for E.coli harboring SPC mutant. In contrast, the host cell overexpressing CPS mutant became more sensitive to H(2)O(2). Some studies on the level of inclusion body revealed that wt GcGAST and SPC mutant embedded in Inclusion bodies (IB) could effectively eliminate H(2)O(2), whereas the mutagenesis to Ser of the second Cys residue in CPC sequence gave rise to the compete loss of H(2)O(2)-eliminating ability. Fourier transform Infrared spectroscopy analysis indicated that the IB of CPS mutant contained more ?-sheet secondary structure than wt and SPC mutant. Non-reducing SDS-PAGE combined western-blotting analysis revealed that the disulfide bonds were important for the formation of IBs of wt GcGAST and SPC mutant, whereas non-reducing SDS-PAGE of resolubilized IBs showed that hydrophobic interaction favored the aggregation of IBs in CPS mutant. Taken together, these results suggested that GcGAST possessed antioxidant activity in the level of IB, which made some contribution to cellular resistance to H(2)O(2). More importantly, the second cysteine residue in CPC sequence was more essential for its antioxidant biological function.
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Application of multiple parallel perfused microbioreactors: Synthesis, characterization and cytotoxicity testing of the novel rare earth complexes with indole acid as a ligand.
J. Inorg. Biochem.
PUBLISHED: 01-09-2013
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Three novel complexes, [La(phen)2(IAA)2]·NO3 (1), [Sm(phen)2(IAA)2]·NO3 (2) and [Sm(IBA)3(phen)]·phen·HNO3·H2O (3) (phen: 1,10-phenanthroline, IAA: indole-3-acetic acid, IBA: indole-3-butyric acid), were synthesized and characterized with spectroscopy (infrared and UV-visible), X-ray crystal diffraction and elemental analysis. Structural analysis revealed that each lanthanide atom in complexes 1-3 held a distorted tricapped trigonal prism geometry in a nine-coordinate mode. There were two types of coordination modes of the IAA ligand in complexes 1 and 2: a ?2-?(1):?(2) bridging mode linking two lanthanide atoms and a ?2-?(1):?(1) double monodentate bridging mode. There were three types of coordination modes of the IBA ligand: a ?2-?(1):?(1) double monodentate bridging mode, a ?1-?(2) bridging mode and a ?2-?(1):?(2) bridging mode linking two lanthanide atoms. Adjacent Sm atoms were linked via the ?2-bridging carboxylate groups of the IBA ligands to generate a binuclear building unit. The biological activity of the complexes was evaluated in human adipose tissue-derived stem cells (hADSCs) and Chang liver cells using a multiple parallel perfused microbioreactor. The results showed that cytotoxicity increased as the concentrations of complexes 1-3 increased.
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Preconcentration and determination of polybrominated diphenyl ethers in environmental water samples by solid-phase microextraction with Fe3O4-coated bamboo charcoal fibers prior to gas chromatography-mass spectrometry.
Anal. Chim. Acta
PUBLISHED: 01-06-2013
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In this paper, bamboo charcoals were modified using Fe3O4 nanosheets for the first time. The composites, as a novel solid-phase microextraction (SPME) fiber coating, were used for the extraction of seven polybrominated diphenyl ethers (PBDEs) in environmental water samples. The extraction factors (stirring rate, extraction time, and ionic strength) and desorption factors (desorption time and desorption temperature) of the fibers were systematically investigated and optimized. Under optimum conditions, the linear range was 1-1000 ng L(-1). Based on the ratio of chromatographic signal to base line noise (SN(-1)=3), the limits of detection (LODs) can reach 0.25-0.62 ng L(-1). The novel method was successful in the analysis of PBDEs in real environmental water samples. The results indicate that bamboo charcoal/Fe3O4 as an SPME coating material coupled with gas chromatography-negative chemical ionization-mass spectrometry is an excellent method for the routine analysis of PBDEs at trace levels in environmental water samples.
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Global Distribution of Bartonella Infections in Domestic Bovine and Characterization of Bartonella bovis Strains Using Multi-Locus Sequence Typing.
PLoS ONE
PUBLISHED: 01-01-2013
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Bartonella bovis is commonly detected in cattle. One B. bovis strain was recently isolated from a cow with endocarditis in the USA, suggesting its role as an animal pathogen. In the present study, we investigated bartonella infections in 893 cattle from five countries (Kenya, Thailand, Japan, Georgia, and Guatemala) and 103 water buffaloes from Thailand to compare the prevalence of the infection among different regions and different bovid hosts. We developed a multi-locus sequence typing (MLST) scheme based on nine loci (16S rRNA, gltA, ftsZ, groEL, nuoG, ribC, rpoB, ssrA, and ITS) to compare genetic divergence of B. bovis strains, including 26 representatives from the present study and two previously described reference strains (one from French cows and another from a cow with endocarditis in the USA). Bartonella bacteria were cultured in 6.8% (7/103) of water buffaloes from Thailand; all were B. bovis. The prevalence of bartonella infections in cattle varied tremendously across the investigated regions. In Japan, Kenya, and the Mestia district of Georgia, cattle were free from the infection; in Thailand, Guatemala, and the Dusheti and Marneuli districts of Georgia, cattle were infected with prevalences of 10-90%. The Bartonella isolates from cattle belonged to three species: B. bovis (n=165), B. chomelii (n=9), and B. schoenbuchensis (n=1), with the latter two species found in Georgia only. MLST analysis suggested genetic variations among the 28 analyzed B. bovis strains, which fall into 3 lineages (I, II, and III). Lineages I and II were found in cattle while lineage III was restricted to water buffaloes. The majority of strains (17/28), together with the strain causing endocarditis in a cow in the USA, belonged to lineage I. Further investigations are needed to determine whether B. bovis causes disease in bovids.
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Synthesis, structure, DNA-binding properties and antioxidant activity of a nickel(II) complex with bis(N-allylbenzimidazol-2-ylmethyl)benzylamine.
J. Photochem. Photobiol. B, Biol.
PUBLISHED: 11-09-2011
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A V-shape ligand bis(N-allylbenzimidazol-2-ylmethyl)benzylamine (babb) and its nickel complex, [Ni(babb)(2)](pic)(2) (pic=picrate), have been synthesized and characterized by physico-chemical and spectroscopic methods. Single-crystal X-ray revealed that the coordination sphere around Ni(II) is distorted octahedral with a N(6) ligand set, in which six nitrogen atoms were afforded by two tridentate ligand babb. The DNA-binding properties of the free ligand babb and Ni(II) complex have been investigated by electronic absorption, fluorescence, and viscosity measurements. The results suggest that babb and Ni(II) complex both bind to DNA via an intercalative binding mode, and the affinity for DNA is more strong in case of Ni(II) complex when compared with babb. The intrinsic binding constants (K(b)) of the Ni(II) complex and ligand with DNA were 3.65×10(4) M(-1) and 2.26×10(3) M(-1), respectively. Additionally, Ni(II) complex also exhibited potential antioxidant properties in vitro studies.
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Synthesis and characterization of the ligand based on benzimidazole and its copper complex: DNA binding and antioxidant activity.
Bioinorg Chem Appl
PUBLISHED: 07-29-2011
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A new copper(II) complex with formulae of [Cu(buobb)(2)](pic)(2), where buobb stands for the ligand of 1,3-bis(1- butylbenzimidazol-2-yl)-2-oxopropane and pic represents 2,4,6-trinitrophenol, has been synthesized and characterized by elemental analyses, molar conductivity, IR, UV-Vis spectra measurements, and cyclic voltammetry. The crystal structure of the copper(II) complex has been determined by X-ray single-crystal diffraction. The coordination environment around each copper(II) atom can be described as a distorted octahedral geometry. The ?-? stacking interactions link the copper(II) complex into a 1D infinite network. The interactions of the ligand and the copper(II) complex with calf thymus DNA (CT-DNA) are investigated by using electronic absorption titration, ethidium bromide-DNA displacement experiments, and viscosity measurements. Additionally, the copper(II) complexs antioxidant properties have been investigated in vitro.
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A V-shaped ligand 1,3-bis(1-methylbenzimidazol-2-yl)-2-oxapropane and its Cu(II) complex: synthesis, crystal structure, antioxidation and DNA-binding properties.
J. Photochem. Photobiol. B, Biol.
PUBLISHED: 07-28-2011
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A six-coordinate copper(II) complex with the ligand 1,3-bis(1-methylbenzimidazol-2-yl)-2-oxopropane (Meobb), with composition [Cu(Meobb)(2)](NO(3))(2)·2CH(3)OH, has been synthesized and characterized by elemental analysis, electrical conductivities, IR, UV-Vis spectral measurements. A study of the electro-chemistry of the copper(II) complex was carried out by using cyclic voltammetry. The molecular structures of the ligand Meobb and the Cu(II) complex were determined by X-ray crystal diffraction. The DNA-binding modes of the ligand and the complex were investigated by electronic absorption titration, ethidium bromide-DNA displacement experiments and viscosity measurements. The experimental evidence indicated the compounds interact with calf thymus DNA through intercalation. Additionally, the Cu(II) complex exhibited potential antioxidant properties in in vitro studies.
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[Study on the relation between peripheral arterial disease and renal insufficiency in high risk cardiovascular patients].
Zhonghua Liu Xing Bing Xue Za Zhi
PUBLISHED: 07-26-2011
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This study was to examine the relation of peripheral arterial disease (PAD) and renal insufficiency in cardiovascular patients with high risk.
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Bartonella spp. in Bats, Guatemala.
Emerging Infect. Dis.
PUBLISHED: 07-19-2011
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To better understand the role of bats as reservoirs of Bartonella spp., we estimated Bartonella spp. prevalence and genetic diversity in bats in Guatemala during 2009. We found prevalence of 33% and identified 21 genetic variants of 13 phylogroups. Vampire bat-associated Bartonella spp. may cause undiagnosed illnesses in humans.
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Bartonella seroprevalence in rural Thailand.
Southeast Asian J. Trop. Med. Public Health
PUBLISHED: 06-29-2011
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We estimated the prevalence of anti-Bartonella antibodies among febrile and non-febrile patients presenting to community hospitals in rural Thailand from February 2002 through March 2003. Single serum specimens were tested for IgG titers to four Bartonella species, B. henselae, B. quintana, B. elizabethae and B. vinsonii subsp vinsonii using an indirect immunofluorescent assay. A titer 21:256 was considered positive. Forty-two febrile patients (9.9%) and 19 non-febrile patients (19%) had positive serology titers to at least one Bartonella species. Age-standardized Bartonella seroprevalence differed significantly between febrile (10%) and non-febrile patients (18%, p=0.047), but did not differ by gender. Among all 521 patients, IgG titers 21:256 to B. henselae were found in 20 participants (3.8%), while 17 (3.3%) had seropositivity to B. quintana, 51 (9.8%) to B. elizabethae, and 19 (3.6%) to B. vinsonii subsp vinsonii. These results suggest exposure to Bartonella species is more common in rural Thailand than previously suspected.
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Synthesis, Crystal Structure, and Spectra Properties of the Cadmium (II) Complex with Bis(N-allylbenzimidazol-2-ylmethyl)benzylamine.
Bioinorg Chem Appl
PUBLISHED: 05-27-2011
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A novel complex of cadmium (II) picrate (pic) with V-shaped ligand bis(N-allylbenzimidazol-2-ylmethyl)benzylamine (babb), with composition [Cd(babb)(2)](pic)(2), was synthesized and characterized by elemental analyses and electrical conductivity, IR, and UV/visible spectra. The crystal structure of the complex has been determined by the single-crystal X-ray diffraction. In the complex, the coordination sphere around Cd (II) is distorted octahedral, six nitrogen atoms involved in coordination afforded by two tridentate ligand babb. Moreover, The DNA-binding properties of the ligand babb and Cd (II) complex were investigated by spectrophotometric methods and viscosity measurements, and the results suggest that they bind to DNA via an intercalation binding mode, and the Cd (II) complex shows higher affinity than the ligand.
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Synthesis, Crystal Structure, and DNA-Binding Studies of a Nickel(II) Complex with the Bis(2-benzimidazolymethyl)amine Ligand.
Bioinorg Chem Appl
PUBLISHED: 05-20-2011
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A V-shaped ligand Bis(2-benzimidazolymethyl)amine (bba) and its nickel(II) picrate (pic) complex, with composition [Ni(bba)(2)](pic)(2)·3MeOH, have been synthesized and characterized on the basis of elemental analyses, molar conductivities, IR spectra, and UV/vis measurements. In the complex, the Ni(II) ion is six-coordinated with a N(2)O(4) ligand set, resulting in a distorted octahedron coordination geometry. In addition, the DNA-binding properties of the Ni(II) complex have been investigated by electronic absorption, fluorescence, and viscosity measurements. The experimental results suggest that the nickel(II) complex binds to DNA by partial intercalation binding mode.
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Bis[N,N-bis-(1-allyl-1H-benzimidazol-2-ylmethyl-?N)benzyl-amine-?N]cadmium dipicrate.
Acta Crystallogr Sect E Struct Rep Online
PUBLISHED: 05-16-2011
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The crystal structure of the title compound, [Cd(C(29)H(29)N(5))(2)](C(6)H(2)N(3)O(7))(2), consists of Cd(II) complex cations and picrate anions. In the complex cation, the Cd(II) ion is chelated by two bis-(1-allyl-benzimidazol-2-ylmeth-yl)benzyl-amine (babb) ligands in a distorted octa-hedral geometry. Extensive C-H?O hydrogen bonding occurs between cations and anions in the crystal structure.
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1,3-Bis(1-methyl-1H-benzimidazol-2-yl)-2-oxapropane.
Acta Crystallogr Sect E Struct Rep Online
PUBLISHED: 05-03-2011
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In the title mol-ecule, C(18)H(18)N(4)O, the dihedral angle between the mean planes of the two benzimidazole ring systems is 61.5?(1)°.
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Characteristics of circle of Willis variations in the mongolian gerbil and a newly established ischemia-prone gerbil group.
ILAR J
PUBLISHED: 04-02-2011
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Mongolian gerbils (Meriones unguiculatus) have an incomplete circle of Willis (CoW), as a result of which approximately 30-40% of these animals develop focal cerebral ischemia after unilateral carotid occlusion (UCO). There are four types of patterns of the anterior and posterior communicating arteries (ACoAs and PCoAs, respectively) of the CoW and they determine the severity of the ischemic symptoms. We used 398 gerbils from five generations, including a selectively bred ischemia-prone group, to investigate post-UCO ischemic symptoms and possible correlations of ACoA and PCoA patterns between parents and their progeny. We observed that if the parents had complete ACoAs, their progeny also had complete ACoAs, and we found significant differences when the parents ACoAs were incomplete: in 60.4% of offspring the type of ACoA was consistent with that of the mother and in 48.2% it was consistent with that of the father. The severity of the neurological symptoms after UCO was significantly related to the patterns of the ACoAs when PCoAs were absent. The proportion of UCO ischemia in gerbils with incomplete ACoAs was significantly higher than in those with complete ACoAs. After selectively breeding five generations, the proportion of UCO ischemia increased from 40% in the F1 animals to 75% in the F5 animals. Our results suggest that variations in the CoW are genetic and demonstrate that we successfully established an ischemia-prone group of gerbils.
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The first reported case of Bartonella endocarditis in Thailand.
Infect Dis Rep
PUBLISHED: 03-08-2011
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Bartonella species have been shown to cause acute, undifferentiated fever in Thailand. A study to identify causes of endocarditis that were blood culture-negative using routine methods led to the first reported case in Thailand of Bartonella endocarditis A 57 year-old male with underlying rheumatic heart disease presented with severe congestive heart failure and suspected infective endocarditis. The patient underwent aortic and mitral valve replacement. Routine hospital blood cultures were negative but B. henselae was identified by serology, PCR, immunohistochemistry and specific culture techniques.
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Amelioration of experimental autoimmune myasthenia gravis rats by blood purification treatment using 4-mercaptoethylpyridine-based adsorbent.
J Biomed Mater Res A
PUBLISHED: 03-04-2011
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The role of immunoadsorption therapy is well established in the management of myasthenia gravis (MG), an autoimmune disorder characterized by muscle weakness and caused by circulating IgG antibodies with specificity against the acetylcholine receptor. Conventional immunoadsorbents that employ recombinant protein A as immobilized ligand suffer from the drawbacks of high cost and low stability. The objective of this work is to assess the safety and efficacy of a synthetic adsorbent for treating MG. Adsorption columns were prepared from a Sepharose-based adsorbent coupled to 4-mercaptoethylpyridine (MEP), which acted as immobilized ligands. Animal model of experimental autoimmune MG (EAMG) using Lewis rats was developed and treated by whole blood perfusion. The results showed that the treatments provided a significant amelioration of clinical weakness for EAMG rats, with clinic score decreasing from 2.08 ± 0.38 to 1.25 ± 0.27. After a treatment session of about 1.5 h, blood cell counts were not significantly changed. Serum levels of total IgG and acetylcholine receptor antibody were reduced by 37.1 ± 6.5% and 35.6 ± 8.6%, respectively. In addition, reduction in complement components C3 (47.1 ± 6.7%), C4 (34.3 ± 3.4%), inflammatory cytokines interleukin-17 (10.4 ± 2.7%), and tumor necrosis factor-? (8.2 ± 3.1%) were also observed. This study demonstrated that MEP-based adsorbent not only removed pathogenic autoantibodies directly from the blood as with protein A adsorbents but also modulated cellular immunity through removal of complement components and related proinflammatory cytokines, thereby providing a potentially superior strategy for the treatment of MG.
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Genome-wide association identifies a susceptibility locus for coronary artery disease in the Chinese Han population.
Nat. Genet.
PUBLISHED: 02-10-2011
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Coronary artery disease (CAD) causes more than 700,000 deaths each year in China. Previous genome-wide association studies (GWAS) in populations of European ancestry identified several genetic loci for CAD, but no such study has yet been reported in the Chinese population. Here we report a three-stage GWAS in the Chinese Han population. We identified a new association between rs6903956 in a putative gene denoted as C6orf105 on chromosome 6p24.1 and CAD (P = 5.00 × 10?³, stage 2 validation; P = 3.00 × 10?³, P = 1.19 × 10?? and P = 4.00 × 10?³ in three independent stage 3 replication populations; P = 4.87 × 10?¹², odds ratio = 1.51 in the combined population). The minor risk allele A of rs6903956 is associated with decreased C6orf105 mRNA expression. We report the first GWAS for CAD in the Chinese Han population and identify a SNP, rs6903956, in C6orf105 associated with susceptibility to CAD in this population.
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Persistent infection or successive reinfection of deer mice with Bartonella vinsonii subsp. arupensis.
Appl. Environ. Microbiol.
PUBLISHED: 01-14-2011
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Bartonella infections are common in rodents. From 1994 to 2006, longitudinal studies of a rodent community, consisting mainly of deer mice (Peromyscus maniculatus), were conducted in southwestern Colorado to study hantaviruses. Blood samples from deer mice captured one or more times during the period 2003 to 2006 (n = 737) were selected to study bartonellae in deer mice. Bartonellae were found to be widely distributed in that population, with an overall prevalence of 82.4% (607/737 mice). No correlation was found between bartonella prevalence and deer mouse weight or sex. Persistent or successive infections with bartonellae were observed in deer mice captured repeatedly, with a prevalence of 83.9% (297/354), and the infection appeared to last for more than 1 year in some of them. Persistent infection with bartonellae may explain the high prevalence of these bacteria in deer mice at this site and, perhaps, elsewhere. Genetic analysis demonstrated that deer mouse-borne bartonella isolates at this site belong to the same species, B. vinsonii subsp. arupensis, demonstrating a specific relationship between B. vinsonii subsp. arupensis and deer mice.
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[NK-T cell activator (?-GalCer) accelerates immune and hematological reconstitution after murine allo-bone marrow transplantation].
Zhongguo Shi Yan Xue Ye Xue Za Zhi
PUBLISHED: 12-24-2010
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Immune reconstitution is crucially relevant for patients receiving hematopoietic stem cell transplantation (HSCT). This study was purposed to investigate the ability of ?-GalCer (?-galactosylceramide), a well-known activator of natural killer T cells (NK-T), to enhance immune and hematological reconstitution. Lethally irradiated BALB/c mice were transplanted with allogeneic C57BL/6 bone marrow cells and splenocytes. ?-GalCer was administered immediately after HSCT. After transplantation, the weight, activity, hairs, diarrhea and survival time of mice were observed daily; the blood routine test was performed once weekly; the donor chimeras, amount of mononuclear cells in spleen (MNC) and relative levels of CD3(+), CD4(+), CD8(+), B220(+), CD11c(+), CD40(+), CD86(+) and CD80(+) cells were detected by FACS on day 2, 7, 14, 27, 70 after transplantation. The results indicated that the MNC counts and relative levels of CD3(+) and CD4(+) in group treated with ?-GalCer on day 2 after transplantation were higher than those in control group; at the same time, the detected donor chimeras were complete recipient type chimeras, then gradually transformed into donor type, on day 7 - 14 donor chimeras in ?-GalCer group were enhanced significantly as compared with control group, on day 27 the chimeras in two groups were complete donor type chimeras thereafter to day 70, the MNC count and relative levels of CD3(+), CD4(+), CD8(+), B220(+), CD40(+), CD86(+) cells in ?-GalCer group were obviously higher than those in control group, at the same time, the hematopoietic reconstitution in ?-GalCer group was accelerated as compared with control group. It is concluded that the ?-GalCer administration after allogeneic bone marrow transplantations accelerates immune and hematological reconstitution.
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Bartonella spp. in bats, Kenya.
Emerging Infect. Dis.
PUBLISHED: 12-03-2010
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We report the presence and diversity of Bartonella spp. in bats of 13 insectivorous and frugivorous species collected from various locations across Kenya. Bartonella isolates were obtained from 23 Eidolon helvum, 22 Rousettus aegyptiacus, 4 Coleura afra, 7 Triaenops persicus, 1 Hipposideros commersoni, and 49 Miniopterus spp. bats. Sequence analysis of the citrate synthase gene from the obtained isolates showed a wide assortment of Bartonella strains. Phylogenetically, isolates clustered in specific host bat species. All isolates from R. aegyptiacus, C. afra, and T. persicus bats clustered in separate monophyletic groups. In contrast, E. helvum and Miniopterus spp. bats harbored strains that clustered in several groups. Further investigation is needed to determine whether these agents are responsible for human illnesses in the region.
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Pulsatile flow improves cerebral blood flow in pediatric cardiopulmonary bypass.
Artif Organs
PUBLISHED: 11-25-2010
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The objective of this study was to evaluate the effect of pulsatile flow on cerebral blood flow (CBF) in infants with the use of a mild hypothermic cardiopulmonary bypass (CPB). Thirty infants scheduled for open heart surgery were randomized to the pulsatile group (Group P, n?=?15) and nonpulsatile group (Group NP, n?=?15). In Group P, pulsatile perfusion was applied during the aortic cross-clamping period, whereas nonpulsatile perfusion was used in Group NP. The systolic peak velocity (Vs), the end of diastolic velocity (Vd), the mean velocity (Vm), and the pulsatility index (PI) and the resistance index (RI) of the middle cerebral artery were measured by a transcranial Doppler (TCD) ultrasound after anesthesia (T1; baseline), at the beginning of CPB (T2), 10?min after aortic cross-clamping (T3), 3?min after declamping (T4), at the cessation of CPB (T5), and at the end of the operation (T6). During T3 and T4, the Vs in Group P was significantly higher than in Group NP. However, there were no statistically significant differences between Vd and Vm. The PI and RI in Group P were also higher than those in Group NP (both P?
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Isolation of Bartonella capreoli from elk.
Vet. Microbiol.
PUBLISHED: 07-30-2010
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The aim of the present study was to investigate the presence of Bartonella infections in elk populations. We report the isolation of four Bartonella strains from 55 elk blood samples. Sequencing analysis demonstrated that all four strains belong to Bartonella capreoli, a bacterium that was originally described in the wild roe deer of Europe. Our finding first time demonstrated that B. capreoli has a wide geographic range, and that elk may be another host for this bacterium. Further investigations are needed to determine the impact of this bacterium on wildlife.
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A de novo mutation in NKX2.5 associated with atrial septal defects, ventricular noncompaction, syncope and sudden death.
Clin. Chim. Acta
PUBLISHED: 07-26-2010
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Mutations in transcription factor NKX2.5 cause congenital heart disease (CHD). We identified a CHD family with atrial septal defects (ASDs), atrioventricular block, ventricular noncompaction, syncope and sudden death. Our objective is to identify the disease-causing mutation in the CHD family.
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Synthesis, crystal structure and photoelectric property of two new coordination polymers constructed by longer-spanning suberic acid and 4,4-bipyridine ligands.
Spectrochim Acta A Mol Biomol Spectrosc
PUBLISHED: 07-02-2010
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Two-dimensional coordination polymers, [M(C?H??O?)(C??H?N?)]·H?O [M=Co (1), Cd (2); C??H?N? = 4,4-bipyridine, C?H??O?=subaric acid] were obtained from the reaction of the metal salts, bipy and subaric acid at 180°C and characterized by elemental analysis, infrared spectrum, and single-crystal X-ray diffraction and surface photovoltage spectrum (SPS). The single-crystal X-ray diffraction showed that the subaric ligand in the two complexes exhibits two types of modes coordinating to transition metal ions, resulting in the formation of a 1D infinite chain along the c-axis. In addition, the results of SPS for complexes 1 and 2 indicate that these two complexes exhibit positive surface photovoltage responses in the range of 300-800 nm, which can be assigned to LMCT and MLCT, respectively. And the SPS of complex 1 also can be assigned to the d?d* electronic transition. The SPS spectra of the two complexes are consistent with their UV-vis spectra.
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Identification of Bartonella infections in febrile human patients from Thailand and their potential animal reservoirs.
Am. J. Trop. Med. Hyg.
PUBLISHED: 06-04-2010
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To determine the role of Bartonella species as causes of acute febrile illness in humans from Thailand, we used a novel strategy of co-cultivation of blood with eukaryotic cells and subsequent phylogenetic analysis of Bartonella-specific DNA products. Bartonella species were identified in 14 blood clots from febrile patients. Sequence analysis showed that more than one-half of the genotypes identified in human patients were similar or identical to homologous sequences identified in rodents from Asia and were closely related to B. elizabethae, B. rattimassiliensis, and B. tribocorum. The remaining genotypes belonged to B. henselae, B. vinsonii, and B. tamiae. Among the positive febrile patients, animal exposure was common: 36% reported owning either dogs or cats and 71% reported rat exposure during the 2 weeks before illness onset. The findings suggest that rodents are likely reservoirs for a substantial portion of cases of human Bartonella infections in Thailand.
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[Effects of electroacupuncture on hepatic blood flow and lipid peroxidation in septic rats].
Zhongguo Zhen Jiu
PUBLISHED: 06-04-2010
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To investigate the protection effects of electroacupuncture on injury of lipid peroxidation induced by liver ischemia in septic rats.
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Detection of multiple Bartonella species in digestive and reproductive tissues of fleas collected from sympatric mammals.
ISME J
PUBLISHED: 03-11-2010
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At least 12 species in the genus Bartonella are zoonotic pathogens that may be transmitted among mammalian hosts by fleas or other arthropods. Apparent host specificity by some Bartonella species to mammalian hosts has been observed, and the detection of multiple Bartonella species in mammalian fleas suggests that fleas take bloodmeals from a variety of host species. However, many flea species are observed to parasitize a narrow host range. Therefore, we suspect that fleas may acquire Bartonella by a mechanism other than ingesting infectious blood. We found that detection of multiple Bartonella genotypes and species is apparently common in fleas and that the majority of fleas tested (5/9) carried Bartonella species atypical of their hosts. We also detected Bartonella DNA in flea reproductive tissues, suggesting that vertical transmission of this organism in vectors is possible, potentially leading to the accumulation of Bartonella diversity over time within fleas.
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Molecular cloning and characterization of the porcine FTO promoter and coding regions.
Mol. Biol. Rep.
PUBLISHED: 02-23-2010
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The fat mass and obesity associated gene (FTO) has been widely reported to be associated with fat mass or fat deposition in different species. In the present study, we cloned both promoter and coding regions of the gene in pigs with over 5 Kb of sequence for the former region and 1,596 bp for the latter region. Comparative analysis of the promoter region among 20 species including pig revealed four conserved regions that harbor transcriptional factors involved in adipose differentiation. Using a pooled DNA sequencing approach, we discovered 39 single nucleotide polymorphisms (SNPs) in the pig FTO gene and four of them were genotyped on 716 pigs representing 3 European and 18 Chinese indigenous pig breeds plus samples of wild boars. We found that TCGG is a favored haplotype in Chinese wild boars and 16 indigenous pig breeds, while Li Cha Black and Tong Cheng pigs had the specific dominant haplotypes of TTGG and TCGA, respectively. ATGA was the main haplotype found in Large White and Duroc pigs, but the haplotype of ATGG was the major type in Landrace. Taken together, these data provide a valuable foundation for the community to fully study the function of FTO gene in pigs.
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Enrichment culture and molecular identification of diverse Bartonella species in stray dogs.
Vet. Microbiol.
PUBLISHED: 02-08-2010
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Using pre-enrichment culture in Bartonella alpha-Proteobacteria growth medium (BAPGM) followed by PCR amplification and DNA sequence identification that targeted a fragment of the citrate synthase gene (gltA), we provide evidence of common bartonella infections and diverse Bartonella species in the blood of stray dogs from Bangkok and Khon Kaen, Thailand. The overall prevalence of all Bartonella species was 31.3% (60/192), with 27.9% (31/111) and 35.8% (29/81) in the stray dogs from Bangkok and Khon Kaen, respectively. Phylogenetic analyzes of gltA identified eight species/genotypes of Bartonella in the blood of stray dogs, including B. vinsonii subsp. arupensis, B. elizabethae, B. grahamii, B. quintana, B. taylorii, and three novel genotypes (BK1, KK1 and KK2) possibly representing unique species with ? 90.2% similarities to any of the known Bartonella species B. vinsonii subsp. arupensis was the only species detected in dogs from both sites, B. quintana and BK1 were found in the dogs from Bangkok, B. elizabethae, B. taylorii, KK1 and KK2 were found in the dogs from Khon Kaen. We conclude that stray dogs in Thailand are frequently infected with Bartonella species that vary with geographic region. As some Bartonella species detected in the present study are considered pathogenic for humans, stray dogs in Thailand may serve as possible reservoirs for Bartonella causing human illnesses. Further work is needed to determine the role of those newly discovered Bartonella genotypes/species in human and veterinary medicine.
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Are carnivores universally good sentinels of plague?
Vector Borne Zoonotic Dis.
PUBLISHED: 12-30-2009
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Sylvatic plague, caused by the bacterium Yersinia pestis, is a flea-borne disease that primarily affects rodents but has been detected in over 200 mammal species worldwide. Mammalian carnivores are routinely surveyed as sentinels of local plague activity, since they can present antibodies to Y. pestis infection but show few clinical signs. In Boulder County, Colorado, USA, plague epizootic events are episodic and occur in black-tailed prairie dogs. Enzootic hosts are unidentified as are plague foci. For three years, we systematically sampled carnivores in two distinct habitat types to determine whether carnivores may play a role in maintenance or transmission of Y. pestis and to identify habitats associated with increased plague prevalence. We sampled 83 individuals representing six carnivore species and found only two that had been exposed to Y. pestis. The low overall rate of plague exposure in carnivores suggests that plague may be ephemeral in this study system, and thus we cannot draw any conclusions regarding habitat-associated plague foci or temporal changes in plague activity. Plague epizootics involving prairie dogs were confirmed in this study system during two of the three years of this study, and we therefore suggest that the targeting carnivores to survey for plague may not be appropriate in all ecological systems.
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[Proton magnetic resonance spectroscopy of the thalamus and hypothalamus in patients with first-episode depression].
Nan Fang Yi Ke Da Xue Xue Bao
PUBLISHED: 12-26-2009
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To investigate the presence of abnormal metabolism in the thalamus and hypothalamus in patients with first-episode depression.
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[Study on the interaction of levofloxacin and MCM-41 by fluorescence spectrum].
Guang Pu Xue Yu Guang Pu Fen Xi
PUBLISHED: 12-03-2009
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The mesoporous molecular sieve MCM-41 was synthesized by hydrothermal method under microwave irradiation and the levofloxacin was encapsulated in the hexagonal ordered channels of MCM-41 using pickling process. The structure and property of MCM-41 and LVFX/MCM-41 were characterized by means of small-angle X-ray diffraction(XRD), Fourier transform infrared spectroscopy (FTIR), N2 physical adsorption, and thermogravimetric analysis-differential thermal analysis (TGA-DTA). The pore diameter and the specific surface area of the mesoporous molecular sieve MCM-41 synthesized under microwave irradiation were 2.382 nm and 1015 m2 x g(-1) respectively. The fluorescence spectra of MCM-41, LVFX/MCM-41, LVFX (solid) and LVFX (solution) were investigated. The results indicated that there were some visible red shifts in the fluorescence spectrum of the composite of LVFX/MCM-41 as compared with the samples before being encapsulated. It was inferred from the results that hydrogen bonds were formed between levofloxacin and the hydroxy group in the inner pores, and the electron cloud could transfer from the hydroxy group to electro-attracting group. At the same time, the electron cloud could transfer in much larger annulations, and the conjugated system of LVFX was extended because of the new annulations formed, which contributed to the red shifts of the fluorescence spectra. The strong interactions between the LVFX and MCM-41 provided the theory for developing a novel delayed release drug using MCM-41 as the carriers.
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JoVE Visualize is a tool created to match the last 5 years of PubMed publications to methods in JoVE's video library.

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In developing our video relationships, we compare around 5 million PubMed articles to our library of over 4,500 methods videos. In some cases the language used in the PubMed abstracts makes matching that content to a JoVE video difficult. In other cases, there happens not to be any content in our video library that is relevant to the topic of a given abstract. In these cases, our algorithms are trying their best to display videos with relevant content, which can sometimes result in matched videos with only a slight relation.