JoVE Visualize What is visualize?
Stop Reading. Start Watching.
Advanced Search
Stop Reading. Start Watching.
Regular Search
Find video protocols related to scientific articles indexed in Pubmed.
Comparison of clinical features between suspected familial colorectal cancer type X and Lynch syndrome in Japanese patients with colorectal cancer: a cross-sectional study conducted by the Japanese Society for Cancer of the Colon and Rectum.
Jpn. J. Clin. Oncol.
PUBLISHED: 11-19-2014
Show Abstract
Hide Abstract
The characteristics of familial colorectal cancer type X are poorly defined. Here we aimed to clarify the differences in clinical features between suspected familial colorectal cancer type X and Lynch syndrome in Japanese patients.
Related JoVE Video
Quality control by photo documentation for evaluation of laparoscopic and open colectomy with D3 resection for stage II/III colorectal cancer: Japan Clinical Oncology Group Study JCOG 0404.
Jpn. J. Clin. Oncol.
PUBLISHED: 08-01-2014
Show Abstract
Hide Abstract
The quality of surgery with D3 resection in randomized controlled clinical trial [Japan Clinical Oncology Group study (JCOG0404)] was assessed by evaluation of the photo documentation of both open and laparoscopic surgeries.
Related JoVE Video
Randomised phase III trial of adjuvant chemotherapy with oral uracil and tegafur plus leucovorin versus intravenous fluorouracil and levofolinate in patients with stage III colorectal cancer who have undergone Japanese D2/D3 lymph node dissection: final results of JCOG0
Eur. J. Cancer
PUBLISHED: 05-21-2014
Show Abstract
Hide Abstract
NSABP C-06 demonstrated the non-inferiority of oral adjuvant uracil and tegafur plus leucovorin (UFT/LV) to weekly fluorouracil and folinate (5-FU/LV) with respect to disease-free survival (DFS) for stage II/III colon cancer. This is the first report of JCOG0205, which compared UFT/LV to standard 5-FU/levofolinate (l-LV) for stage III colorectal cancer patients who have undergone Japanese D2/D3 lymph node dissection.
Related JoVE Video
A phase I trial of preoperative S-1 in combination with oxaliplatin and pelvic radiation for lower rectal cancer with T4 and lateral pelvic lymph node metastasis.
Int. J. Clin. Oncol.
PUBLISHED: 03-17-2014
Show Abstract
Hide Abstract
The purpose of this phase I study of the dose escalation of oxaliplatin in combination with oral S-1 and pelvic radiation preoperatively for poor-risk lower rectal cancer was to determine the dose-limiting toxicity (DLT) and recommended dose of oxaliplatin.
Related JoVE Video
Short-term surgical outcomes from a randomized controlled trial to evaluate laparoscopic and open D3 dissection for stage II/III colon cancer: Japan Clinical Oncology Group Study JCOG 0404.
Ann. Surg.
PUBLISHED: 02-11-2014
Show Abstract
Hide Abstract
A randomized controlled trial to confirm the non-inferiority of laparoscopic surgery to open surgery in terms of overall survival was conducted, and short-term surgical outcomes are demonstrated.
Related JoVE Video
A Randomized Controlled Trial of the Conventional Technique Versus the No-touch Isolation Technique for Primary Tumor Resection in Patients with Colorectal Cancer: Japan Clinical Oncology Group Study JCOG1006.
Jpn. J. Clin. Oncol.
PUBLISHED: 11-08-2013
Show Abstract
Hide Abstract
A randomized controlled trial is currently being conducted in Japan to demonstrate the superiority of the no-touch isolation technique over the conventional technique for patients with potentially curative colon and rectosigmoid cancer. The conventional technique procedure gives first priority to mobilization of the tumor-bearing segment of the colon, which is followed by central vascular ligation and ligation of other vasculature. Conversely, the no-touch isolation technique gives first priority to central vascular ligation, which is followed by mobilization of the tumor-bearing segment of the colon. A total of 850 patients will be enrolled in this trial. The primary endpoint is disease-free survival. Secondary endpoints are overall survival, relapse-free survival, liver metastasis-free survival, mode of recurrence, surgical morbidity, adverse events due to postoperative chemotherapy, serious adverse events and short-term clinical outcomes.
Related JoVE Video
Early detection of metachronous bile duct cancer in Lynch syndrome: report of a case.
Surg. Today
PUBLISHED: 06-03-2013
Show Abstract
Hide Abstract
Lynch syndrome is an autosomal dominant disease associated with a high incidence of colorectal, endometrial, stomach, ovarian, pancreatic, ureter and renal pelvis, bile duct and brain tumors. The syndrome can also include sebaceous gland adenomas and keratoacanthomas, and carcinoma of the small bowel. The lifetime risk for bile duct cancer in patients with Lynch syndrome is approximately 2 %. The present report describes a case of Lynch syndrome with metachronous bile duct cancer diagnosed at an early stage. The patient was a 73-year-old Japanese male who underwent a successful left lobectomy of the liver, and there was no sign of recurrence for 2 years postoperative. However, this patient harbored a germline mutation in MLH1, which prompted diagnostic examinations for noncolorectal tumors when a periodic surveillance blood examination showed abnormal values of hepatobiliary enzymes. Although most patients with bile duct cancer are diagnosed at an advanced stage, the bile duct cancer was diagnosed at an early stage in the present patient due to the observation of the gene mutation and the preceding liver tumor. This case illustrates the importance of continuous surveillance for extracolonic tumors, including bile duct cancer, in patients with Lynch syndrome.
Related JoVE Video
[Treatment of lateral node metastasis from lower rectal cancer].
Nihon Geka Gakkai Zasshi
PUBLISHED: 09-28-2011
Show Abstract
Hide Abstract
Lateral lymphatics of the rectum originate in the area where branches of the inferior hypogastric plexus and the middle rectal vessels from the internal iliac vessels enter the mesorectum below the level of the peritoneal reflection in the pelvis, then reach the bifurcation of iliac vessels along the internal iliac vessels. Among lateral lymph nodes, the middle rectal, obturator, and internal iliac lymph nodes are important from the viewpoint of both the incidence of metastais and treatment effects. Although total mesorectal excision (TME) had become the standard surgical treatment for rectal cancer by the 1990s, this technique does not treat lateral node metastasis. A randomized clinical trial of TME versus D3 lymphadenectomy (JCOG0212) was started in 2003, and the registration of 701 patients with lower rectal cancer was completed in August 2010. The results of this clinical trial are highly anticipated. In Japan, where the rate of local recurrence after surgery is low, patients at high risk of local recurrence such as those with lateral node metastasis, T4 disease, and multiple lymph node metastases in the mesorectum should be selected to receive preoperative chemoradiation. Japanese surgeons who treat rectal cancers are in an advantageous position because they have the additional measure of lateral node dissection along with TME and chemoradiotherapy.
Related JoVE Video
Short-term outcomes of laparoscopic intersphincteric resection for lower rectal cancer and comparison with open approach.
Dig Surg
PUBLISHED: 07-12-2011
Show Abstract
Hide Abstract
To evaluate the short-term surgical outcomes of laparoscopic intersphincteric resection (ISR) for lower rectal cancer, and to compare them with a case-control series of open ISR.
Related JoVE Video
A Phase I/II trial of chemoradiotherapy concurrent with S-1 plus mitomycin C in patients with clinical Stage II/III squamous cell carcinoma of anal canal (JCOG0903: SMART-AC).
Jpn. J. Clin. Oncol.
PUBLISHED: 04-05-2011
Show Abstract
Hide Abstract
A Phase I/II trial of chemoradiotherapy concurrent with S-1 plus mitomycin C in patients with clinical Stage II/III squamous cell carcinoma of the anal canal was started in Japan. The aim of this trial is to determine the recommended dose of S-1 combined with a fixed dose of mitomycin C plus radiotherapy in Phase I and to evaluate the efficacy and safety in Phase II. The primary endpoint for the Phase II part of this study is the proportion of 3-year event-free survival, in which the following are defined as events: disease progression, residual tumor at the end of chemoradiotherapy, colostomy or death, whichever comes first. Secondary endpoints are progression-free survival, proportion of complete response and adverse events. In the Phase II part of this study, a total of 65 patients will be enrolled from 42 institutions over 6 years.
Related JoVE Video
Comparing endoscopic submucosal dissection with transanal resection for non-invasive rectal tumor: a retrospective study.
J. Gastroenterol. Hepatol.
PUBLISHED: 02-09-2011
Show Abstract
Hide Abstract
Endoscopic submucosal dissection (ESD) is an alternative to transanal resection (TAR) in treating rectal adenomas, intramucosal cancers, and superficial submucosal cancers. The purpose of this study is to compare the clinical efficacy between ESD and TAR for non-invasive rectal tumors.
Related JoVE Video
Exosome can prevent RNase from degrading microRNA in feces.
J Gastrointest Oncol
PUBLISHED: 01-16-2011
Show Abstract
Hide Abstract
Because the stability of miRNA in feces has not been clarified, we examined the stability of miRNA in feces.
Related JoVE Video
MicroRNA expression profiling of exfoliated colonocytes isolated from feces for colorectal cancer screening.
Cancer Prev Res (Phila)
PUBLISHED: 10-19-2010
Show Abstract
Hide Abstract
To reduce the colorectal cancer (CRC) mortality rate, we have reported several CRC screening methods using colonocytes isolated from feces. Expression analysis of oncogenic microRNA (miRNA) in peripheral blood was recently reported for CRC detection. In the present study, we conducted miRNA expression analysis of exfoliated colonocytes isolated from feces for CRC screening. Two hundred six CRC patients and 134 healthy volunteers were enrolled in the study. miRNA expression of the miR-17-92 cluster, miR-21, and miR-135 in colonocytes isolated from feces as well as frozen tissues was analyzed by quantitative real-time PCR. The expression of the miR-17-92 cluster, miR-21, and miR-135 was significantly higher in CRC tissues compared with normal tissues. The exfoliated colonocytes of 197 CRC patients and 119 healthy volunteers were analyzed because of the presence of sufficient miRNA concentration. miR-21 expression did not differ significantly between CRC patients and healthy volunteers (P = 0.6). The expression of miR-17-92 cluster and miR-135 was significantly higher in CRC patients than in healthy volunteers (P < 0.0001). The overall sensitivity and specificity by using miRNA expression was 74.1% (146/197; 95% confidence interval, 67.4-80.1) and 79.0% (94/119; 95% confidence interval, 70.6-85.9), respectively. Sensitivity was dependent only on tumor location (P = 0.0001). miRNA was relatively well conserved in exfoliated colonocytes from feces both of CRC patients and healthy volunteers. miRNA expression analysis of the isolated colonocytes may be a useful method for CRC screening. Furthermore, oncogenic miRNA highly expressed in CRC should be investigated for CRC screening tests in the future.
Related JoVE Video
Related JoVE Video
Diverting stoma in rectal cancer surgery. A retrospective study of 329 patients from Japanese cancer centers.
Int J Colorectal Dis
PUBLISHED: 07-22-2010
Show Abstract
Hide Abstract
A diverting stoma (DS) has been constructed for many patients with low anterior resection (LAR), but it is still controversial whether DS can prevent anastomotic leakages. The aim of this study was to investigate the risk factors of anastomotic leakage including DS construction, and to evaluate the clinical course affected by DS according to the necessity of urgent abdominal reoperation for anastomotic leakage.
Related JoVE Video
Significance of PGP9.5 expression in cancer-associated fibroblasts for prognosis of colorectal carcinoma.
Am. J. Clin. Pathol.
PUBLISHED: 06-17-2010
Show Abstract
Hide Abstract
To assess the expression of a cancer-associated fibroblasts (CAFs) marker as an indicator of prognosis, we raised anti-protein gene product 9.5 (PGP9.5) monoclonal antibody against cultured fibroblasts. PGP9.5 expression in cultured normal fibroblasts was increased by transforming growth factor beta stimulation, indicating the phenotypic alteration to activated fibroblast. We immunohistochemically evaluated PGP9.5 expression with the CAFs of 110 colorectal cancer cases under T3 stage. PGP9.5 immunoreactivity in 30% or more of CAFs was defined as high PGP9.5 expression, and the other cases were considered as having low PGP9.5 expression. Patients with high PGP9.5 expression (42.7%) had significantly shorter survival and a higher incidence of recurrence than the low PGP9.5 expression group (P = .002 and P < .001, respectively). Multivariate analysis indicated PGP9.5 expression as an independent prognostic factor for overall and recurrence-free survival partly as well as lymph node metastasis. These results indicate that PGP9.5 expression in CAFs is a helpful finding to represent the overall biologic behavior of advanced colorectal cancer.
Related JoVE Video
Final results of randomized trials by the National Surgical Adjuvant Study of Colorectal Cancer (NSAS-CC).
Cancer Chemother. Pharmacol.
PUBLISHED: 05-01-2010
Show Abstract
Hide Abstract
In the latter 1990s, adjuvant chemotherapy for completely resected Stage III colorectal cancer remained controversial in Japan. We conducted two independent randomized controlled trials in patients with Stage III colon and rectal cancer.
Related JoVE Video
Plasma concentrations of VCAM-1 and PAI-1: a predictive biomarker for post-operative recurrence in colorectal cancer.
Cancer Sci.
PUBLISHED: 04-21-2010
Show Abstract
Hide Abstract
This prospective study used antibody suspension bead arrays to identify biomarkers capable of predicting post-operative recurrence with distal metastasis in patients with colorectal cancer. One hundred colorectal cancer patients who underwent surgery were enrolled in this study. The median follow-up period was 3.9 years. The pre-operative plasma concentrations of 24 angiogenesis-related molecules were analyzed with regard to the TNM stage and the development of post-operative recurrence. The concentrations of half of the examined molecules (13/24) increased significantly according to the TNM stage (P < 0.05). Meanwhile, a multivariate logistic regression analysis revealed that the concentrations of vascular cell adhesion molecule 1 (VCAM-1) and plasminogen activator inhibitor-1 (PAI-1) were significantly higher in the post-operative recurrence group. The VCAM-1 and PAI-1 model discriminated post-operative recurrence with an area under the curve of 0.82, a sensitivity of 0.75, and a specificity of 0.73. A leave-one-out cross-validation was applied to the model to assess the prediction performance, and the result indicated that the cross-validated error rate was 12.5% (12/96). In conclusion, our results demonstrate that antibody suspension bead arrays are a powerful tool to screen biomarkers in the clinical setting, and the plasma levels of VCAM-1 and PAI-1 together may be a promising biomarker for predicting post-operative recurrence in patients with colorectal cancer.
Related JoVE Video
A case of advanced rectal adenocarcinoid tumor with long-term survival.
Jpn. J. Clin. Oncol.
PUBLISHED: 03-25-2010
Show Abstract
Hide Abstract
Adenocarcinoid tumor most commonly occurs in the appendix and a tumor arising in the rectum is extremely rare. A 58-year-old man underwent total pelvic exenteration with extended lateral lymph node dissection for rectal adenocarcinoid tumor invading the urethra with lateral lymph node metastasis. Microscopically and immunohistochemically, the tumor consisted of carcinoid-like components and signet-ring-cell-carcinoma-like components, and an adenocarcinoid tumor was diagnosed. Postoperatively, the patient received combination chemotherapy of fluorouracil and leucovorin as an adjuvant therapy. Three years and 5 years after the initial surgery, the patient developed left groin and left external iliac lymph node recurrences, and lymphadenectomy was performed each time. As a result, the patient is alive more than 5 years after the initial surgery. There is no consensus on the indication of surgical treatment for adenocarcinoid tumor. However, in advanced cases, an aggressive surgical procedure might result in long-term survival when resectable.
Related JoVE Video
Simultaneous laparoscopic descending colectomy and nephroureterectomy for descending colon carcinoma and left ureteral carcinoma: report of a case.
Surg. Today
PUBLISHED: 07-29-2009
Show Abstract
Hide Abstract
To our knowledge, there is no case report of the synchronous resection of colon and ureteral carcinomas by laparoscopy, because of the rareness of this combination and the technical difficulties involved. We report a case of simultaneous descending colon and left ureteral carcinomas, both of which were judged to be relatively early stage carcinoma, which we resected successfully laparoscopically. The patient, a 65-year-old man, recovered uneventfully and was discharged on postoperative day 8. For simultaneous abdominal primary malignancies, laparoscopic surgery should be considered proactively if the procedure is technically feasible and judged to be curative.
Related JoVE Video
Long-term outcome of metachronous rectal cancer following ileorectal anastomosis for familial adenomatous polyposis.
J. Gastrointest. Surg.
PUBLISHED: 07-19-2009
Show Abstract
Hide Abstract
Total colectomy with ileorectal anastomosis (IRA) for familial adenomatous polyposis (FAP) carries a potential risk of metachronous cancer in the residual rectum. This study evaluated the risk of cancer development in the residual rectum.
Related JoVE Video
Prognostic significance of CXCL12 expression in patients with colorectal carcinoma.
Am. J. Clin. Pathol.
PUBLISHED: 07-17-2009
Show Abstract
Hide Abstract
The present study investigated the protein expression level of CXCL12 in colorectal cancer and aimed to elucidate its association with prognosis. CXCL12 positivity in 50% or more of tumor cells was defined as high expression and that in less than 50% of the tumor cells as low expression. CXCL12+ tumor budding at the invasive front was divided into 2 grades: high with 10 or more budding foci per x200 field of view and low grade with fewer than 10 budding foci. Patients with high expression (72.7%) and high grade CXCL12+ tumor budding (43.0%) had significantly shorter survival than patients with low expression (P = .014) and low grade (P = .003), respectively. Patients with a combination of high expression and high grade had the worst outcome (P < .001). Our study demonstrated that CXCL12 expression in colorectal cancer cells and at sites of budding were significant prognostic factors. Furthermore, together with lymph node metastasis, a combination of both expression patterns was a more powerful independent prognostic factor.
Related JoVE Video
Multimedia articles. Small-bowel tumors with extensive mesenteric involvement can be resected with careful dissection of the mesenteric vessels with good outcomes.
Dis. Colon Rectum
PUBLISHED: 07-08-2009
Show Abstract
Hide Abstract
We show the video of the dissection and resection of a locally advanced jejunal adenocarcinoma, with the involvement of multiple loops, to the base of the mesentery. Complete resection was made possible by careful dissection of the jejunal vessels and superior mesenteric artery at the root of the small-bowel mesentery (see Video, Supplemental Digital Content 1, http://links.lww.com/A1308). The long-term outcome for this patient is good.
Related JoVE Video
Laparoscopic surgery for transverse and descending colon carcinomas has comparable safety to laparoscopic surgery for colon carcinomas at other sites.
Dig Surg
PUBLISHED: 06-29-2009
Show Abstract
Hide Abstract
Many randomized clinical trials have been performed to treat colon carcinoma with the exclusion of transverse colon carcinoma or descending colon carcinoma. The aim of the present study was to investigate the difference in surgical outcomes between laparoscopic surgery for transverse/descending colon carcinomas and that for other colon carcinomas.
Related JoVE Video
Optimum lymph node dissection in clinical T1 and clinical T2 colorectal cancer.
Dis. Colon Rectum
PUBLISHED: 06-09-2009
Show Abstract
Hide Abstract
On the basis of a retrospective review, we aimed to clarify the optimum extent of lymph node dissection in patients with cT1 and cT2 colorectal cancer.
Related JoVE Video
Risk factors for anastomotic leakage following intersphincteric resection for very low rectal adenocarcinoma.
J. Gastrointest. Surg.
PUBLISHED: 05-18-2009
Show Abstract
Hide Abstract
The aim of this study was to perform a retrospective analysis of the risk factors for anastomotic leakage following intersphincteric resection (ISR) for very low rectal cancer.
Related JoVE Video
Long-term results of hepatectomy after hepatic arterial infusion chemotherapy for initially unresectable hepatic colorectal metastases.
J. Gastrointest. Surg.
PUBLISHED: 05-18-2009
Show Abstract
Hide Abstract
The prognosis of unresectable hepatic colorectal metastases is poor even if chemotherapy is administered. The purpose of this study was to evaluate the long-term efficacy of hepatic arterial infusion (HAI) chemotherapy and hepatectomy following HAI for such condition.
Related JoVE Video
Male urinary and sexual functions after mesorectal excision alone or in combination with extended lateral pelvic lymph node dissection for rectal cancer.
Ann. Surg. Oncol.
PUBLISHED: 05-15-2009
Show Abstract
Hide Abstract
Mesorectal excision reduced the incidence of genitourinary dysfunction compared with conventional surgery. In Japan, extended lateral pelvic lymph node dissection (ELD) is added to mesorectal excision when lateral pelvic node metastasis is suspected. The aim of this study was to evaluate male genitourinary function after mesorectal excision or mesorectal excision plus ELD for rectal cancer.
Related JoVE Video
Definition and grading of anastomotic leakage following anterior resection of the rectum: a proposal by the International Study Group of Rectal Cancer.
Surgery
PUBLISHED: 04-25-2009
Show Abstract
Hide Abstract
Anastomotic leakage represents a major complication after anterior resection of the rectum. The incidence of anastomotic leakage varies considerably among clinical studies in part owing to the lack of a standardized definition of this complication. The aim of the present article was to propose a definition and severity grading of anastomotic leakage after anterior rectal resection.
Related JoVE Video
A randomized phase II/III trial comparing hepatectomy followed by mFOLFOX6 with hepatectomy alone as treatment for liver metastasis from colorectal cancer: Japan Clinical Oncology Group Study JCOG0603.
Jpn. J. Clin. Oncol.
PUBLISHED: 04-23-2009
Show Abstract
Hide Abstract
A randomized controlled trial is being conducted in Japan to compare hepatectomy alone with hepatectomy followed by adjuvant chemotherapy as treatment in patients with curatively resected liver metastases from colorectal cancer to improve survival with intensive chemotherapy. Between 42 and 70 days after liver resection, patients are randomly assigned to either hepatectomy alone or hepatectomy followed by 12 cycles of modified FOLFOX6 (mFOLFOX6) regimen. A total of 300 patients (including 78 patients in Phase II) will be accrued from 38 institutions within 3 years. The primary endpoint is treatment compliance at nine courses of mFOLFOX6 regimen in Phase II and disease-free survival in Phase III. The secondary endpoints are overall survival, incidence of adverse events and patterns of recurrence.
Related JoVE Video
Risk factors of lateral pelvic lymph node metastasis in advanced rectal cancer.
Int J Colorectal Dis
PUBLISHED: 04-01-2009
Show Abstract
Hide Abstract
To clarify the risk factors of lateral pelvic lymph node (LPLN) metastasis of rectal cancer, we examined associations between LPLN status and clinicopathological factors including LPLN status diagnosed by computed tomography (CT).
Related JoVE Video
Current therapeutic strategies for anal squamous cell carcinoma in Japan.
Int. J. Clin. Oncol.
PUBLISHED: 03-08-2009
Show Abstract
Hide Abstract
In Western countries, chemoradiotherapy (CRT) is well established as the standard therapy for stages II/III anal squamous cell carcinoma (ASCC). In Japan, the therapeutic modalities for and outcomes of this disease have not been clarified because ASCC is quite rare. The Colorectal Cancer Study Group of the Japan Clinical Oncology Group (JCOG-CCSG) conducted a survey to determine the current therapeutic strategies for ASCC in Japan.
Related JoVE Video
Determination of splice-site mutations in Lynch syndrome (hereditary non-polyposis colorectal cancer) patients using functional splicing assay.
Fam. Cancer
PUBLISHED: 03-03-2009
Show Abstract
Hide Abstract
Lynch syndrome (hereditary non-polyposis colorectal cancer) is an inherited disease caused by germ-line mutation in mismatch repair genes such as MLH1, MSH2, and MSH6. The mutations include missense and nonsense mutations, small insertions and deletions, and gross genetic alterations including large deletions and duplications. In addition to these genetic changes, mutations in introns are also involved in the pathogenesis. However, it is sometimes difficult to interpret correctly the pathogenicity of variants in exons as well as introns. To evaluate the effect of splice-site mutations in two Lynch syndrome patients, we carried out a functional splicing assay using minigenes. Consequently, this assay showed that the mutation of c.1731+5G>A in MLH1 led to exon15 skipping, and that the mutation of c.211+1G>C in MSH2 created an activated cryptic splice-site 17-nucleotides upstream in exon1. These aberrant splicing patterns were not observed when wild type sequence was used for the assay. We also obtained concordant results by RT-PCR experiments with transcripts from the patients. Furthermore, additional functional splicing assays using two different intronic mutations described in earlier studies revealed splicing alterations that were in complete agreement with the reports. Therefore, functional splicing assay is helpful for evaluating the effects of genetic variants on splicing.
Related JoVE Video
Podoplanin expression identified in stromal fibroblasts as a favorable prognostic marker in patients with colorectal carcinoma.
Oncology
PUBLISHED: 02-16-2009
Show Abstract
Hide Abstract
The microenvironment of cancer plays a critical role in its progression. However, the molecular features of cancer-associated fibroblasts (CAFs) are less well understood than those of cancer cells. We investigated the clinicopathological significance of podoplanin expression in stromal fibroblasts in patients with colorectal cancer (CRC).
Related JoVE Video
A comparison between the treatment of low rectal cancer in Japan and the Netherlands, focusing on the patterns of local recurrence.
Ann. Surg.
PUBLISHED: 02-13-2009
Show Abstract
Hide Abstract
Differences exist between Japan and The Netherlands in the treatment of low rectal cancer. The purpose of this study is to analyze these, with focus on the patterns of local recurrence.
Related JoVE Video
Pelvic exenteration for clinical T4 rectal cancer: oncologic outcome in 93 patients at a single institution over a 30-year period.
Surgery
PUBLISHED: 01-27-2009
Show Abstract
Hide Abstract
Patients with stage T4 rectal cancer are known to have poor survival and often require pelvic exenteration (PE). We describe the oncologic outcome of PE for patients with clinical T4 rectal cancer over a 30-year period.
Related JoVE Video
Detection of the DNA point mutation of colorectal cancer cells isolated from feces stored under different conditions.
Jpn. J. Clin. Oncol.
PUBLISHED: 01-23-2009
Show Abstract
Hide Abstract
We reported on a novel diagnostic method for colorectal cancer (CRC) using a DNA-based analysis of isolated colonocytes from feces. The aim of the present study was to investigate with real-time PCR and direct sequencing analysis whether the cancer cells could be detected in feces stored under different conditions after evacuation.
Related JoVE Video
The rs6983267 SNP is associated with MYC transcription efficiency, which promotes progression and worsens prognosis of colorectal cancer.
Ann. Surg. Oncol.
Show Abstract
Hide Abstract
The oncogenic single nucleotide polymorphism rs6983267, located on 8q24.21, may affect copy number aberrations and/or expression profiles in colorectal cancer (CRC). We investigated the role of this single nucleotide polymorphism in the clinical outcome of CRC.
Related JoVE Video
Aberrant splicing caused by a MLH1 splice donor site mutation found in a young Japanese patient with Lynch syndrome.
Fam. Cancer
Show Abstract
Hide Abstract
Lynch syndrome, also known as hereditary non-polyposis colorectal cancer, characterized by predisposition to colorectal cancer and other associated cancers, is an autosomal-dominant disorder mainly caused by germline mutations in DNA mismatch repair (MMR) genes such as MLH1, MSH2, and MSH6. Some mutations that disrupt splice donor or acceptor sites cause aberrant mRNA splicing. These mutations are generally considered as pathogenic ones, however, it is sometimes uneasy to accurately predict their pathogenicity without functional assays, particularly when the mutation is a single nucleotide substitution. In this report, we describe a 25-year-old patient with Lynch syndrome who carries a germline variant in a splice donor site of the MLH1 gene (c.790 + 5 G > T), which was first detected among Asian populations. The immunohistochemical analysis revealed loss of MLH1 protein expression in the tumor. Our splicing assay confirmed that the intronic MLH1 variant actually caused aberrant splicing, supporting its pathogenic effect. Our data accumulate more information on the genotype-phenotype relationships in patients with Lynch syndrome.
Related JoVE Video
Risk factors for anastomotic leakage after laparoscopic surgery for rectal cancer using a stapling technique.
Surg Laparosc Endosc Percutan Tech
Show Abstract
Hide Abstract
This study evaluated the risk factors for anastomotic leakage after laparoscopic surgery for rectal cancer using a stapling technique.
Related JoVE Video
Postoperative morbidity and mortality after mesorectal excision with and without lateral lymph node dissection for clinical stage II or stage III lower rectal cancer (JCOG0212): results from a multicentre, randomised controlled, non-inferiority trial.
Lancet Oncol.
Show Abstract
Hide Abstract
Mesorectal excision is the international standard surgical procedure for lower rectal cancer. However, lateral pelvic lymph node metastasis occasionally occurs in patients with clinical stage II or stage III rectal cancer, and therefore mesorectal excision with lateral lymph node dissection is the standard procedure in Japan. We did a randomised controlled trial to confirm that the results of mesorectal excision alone are not inferior to those of mesorectal excision with lateral lymph node dissection.
Related JoVE Video
Genomic copy number of a carcinogenic single nucleotide polymorphism at 8q24 in non-risk allele colorectal cancer associated with insulin growth factor 2 receptor expression.
J. Gastroenterol. Hepatol.
Show Abstract
Hide Abstract
The incidence of both diabetes mellitus and hyperlipidemia is increasing and they are risk factors for colorectal cancer (CRC). On the other hand, the carcinogenic significance of the single nucleotide polymorphism (SNP), rs6983267 at 8q24, in CRC has been reported. The association between the SNP genotype and genes associated with diabetes or hyperlipidemia was investigated in cases of CRC.
Related JoVE Video
Prognostic factors of rectal cancer patients with lateral pelvic lymph node metastasis.
Hepatogastroenterology
Show Abstract
Hide Abstract
We examined the clinicopathological characteristics of rectal cancer patients with lateral pelvic lymph node (LPLN) metastasis in order to clarify their associated prognostic factors.
Related JoVE Video
Anal canal neuroendocrine carcinoma associated with squamous intraepithelial neoplasia: a human papillomavirus 18-related lesion.
Pathol. Int.
Show Abstract
Hide Abstract
Neuroendocrine carcinoma (NEC) of the anal canal is exceedingly rare and its histogenesis is poorly understood. We present a case of small-cell NEC of the anal canal in a 70-year-old woman. The NEC appeared as a submucosal tumor at the dentate line and was associated with squamous intraepithelial neoplasia (SIN). The NEC was positive for neuroendocrine markers including synaptophysin, chromogranin A and CD56, whereas the SIN component did not express any of these markers. Both components exhibited p16 overexpression. A PCR analysis revealed that both the SIN and NEC components were positive for human papillomavirus (HPV) 18 DNA. Our observations imply that SIN may be a precursor of anal canal NEC and that HPV18 may play an important role in the histogenesis of anal canal NEC, similar to its role in cervical NEC.
Related JoVE Video
Clinicopathological characteristics and prognostic factors of advanced colorectal mucinous adenocarcinoma.
Histopathology
Show Abstract
Hide Abstract
Mucinous adenocarcinoma (MUC) is a histological variant of colorectal adenocarcinoma. The aim of the present study was to characterize clinicopathological features and identify prognostic factors of MUCs.
Related JoVE Video
Increased risk for CRC in diabetic patients with the nonrisk allele of SNPs at 8q24.
Ann. Surg. Oncol.
Show Abstract
Hide Abstract
Colorectal cancer (CRC) oncogenesis was considered to be determined by interactions between genetic and environmental factors. Specific interacting factors that influence CRC morbidity have yet to be fully investigated.
Related JoVE Video

What is Visualize?

JoVE Visualize is a tool created to match the last 5 years of PubMed publications to methods in JoVE's video library.

How does it work?

We use abstracts found on PubMed and match them to JoVE videos to create a list of 10 to 30 related methods videos.

Video X seems to be unrelated to Abstract Y...

In developing our video relationships, we compare around 5 million PubMed articles to our library of over 4,500 methods videos. In some cases the language used in the PubMed abstracts makes matching that content to a JoVE video difficult. In other cases, there happens not to be any content in our video library that is relevant to the topic of a given abstract. In these cases, our algorithms are trying their best to display videos with relevant content, which can sometimes result in matched videos with only a slight relation.