JoVE Visualize What is visualize?
Stop Reading. Start Watching.
Advanced Search
Stop Reading. Start Watching.
Regular Search
Find video protocols related to scientific articles indexed in Pubmed.
Prenylated flavonoids from the stems and leaves of Desmodium caudatum and evaluation of their inhibitory activity against the film-forming growth of Zygosaccharomyces rouxii F51.
J. Agric. Food Chem.
PUBLISHED: 06-30-2014
Show Abstract
Hide Abstract
In order to provide scientific evidence for the relationship between the traditional usage, stems and leaves of Desmodium caudatum being used for protecting miso from spoilage, and its Japanese name (miso-naoshi), phytochemical study on the stems and leaves of this plant was carried out. Seven new prenylated flavonoids (1-3, 15-18), together with 19 known compounds (4-14, 19-26), were isolated, and the structures of new compounds were elucidated by extensive spectroscopic analyses. The minimum inhibitory concentrations (MICs) of 28 flavonoids, including 17 compounds (1, 2, 4, 5, 7-14, 20-22, 24, 25) isolated in this study and 11 flavonoids (27-37) previously isolated from the roots of this plant, against the film-forming yeast of Zygosaccharomyces rouxii F51 were determined. Fifteen compounds (2, 4, 5, 11, 12, 14, 21, 22, 25, 27, 28, 32-35) inhibited the film-forming growth of Z. rouxii F51 (MIC values, 7.8-62.5 ?g/mL), among which 2",2"-dimethylpyran-(5",6":7,8)-5,2'-dihydroxy-4'-methoxy-(2R,3R)-dihydroflavonol (11) demonstrated potent inhibitory activity with an MIC value of 7.8 ?g/mL.
Related JoVE Video
Hypelodins A and B, polyprenylated benzophenones from Hypericum elodeoides.
J Nat Med
PUBLISHED: 05-13-2014
Show Abstract
Hide Abstract
Two new polyprenylated benzophenones, hypelodins A (1) and B (2), were isolated from the aerial parts of the Chinese medicinal plant Hyperium elodeoides (Choisy). Their structures were elucidated on the basis of spectroscopic evidence. Hypelodin A (1) is a polyprenylated benzophenone having a tetrahydropyrane ring with three prenyl groups and one 4-methyl-1,3-pentadiene moiety, while hypelodin B (2) has a cage-like structure with a 6/6/5/7/6/5 hexacyclic ring system.
Related JoVE Video
Triterpenoids from the fruits of Azadirachta indica (Meliaceae).
Fitoterapia
PUBLISHED: 08-13-2013
Show Abstract
Hide Abstract
Four new triterpenoids, indicalilacols A-D (1-4), were isolated from the MeOH extract of the fruits of Azadirachta indica, including a new 19(10?9?)abeo-tirucallane derivative, two new tirucallane-type triterpenoids, and a new euphane-type triterpenoid, along with three known tirucallane-type triterpenoids. Their structures were elucidated on the basis of spectroscopic analyses. The absolute configuration of 2 was elucidated by the chemical conversion of 2 into 21-oxo-melianodiol 24,25-acetonide. Compounds 2, 6-8 exhibited moderate cytotoxicity against three human cancer cell lines, including multidrug-resistant (MDR) cancer cells (KB-C2). Although compound 5 was not cytotoxic against any of the tested cancer cell lines, 5 showed cytotoxicity against KB-C2 cells in the presence of 2.5?M colchicine, suggesting that 5 might have an MDR-reversal effect.
Related JoVE Video
Rigenolide A, a new secoiridoid glucoside with a cyclobutane skeleton, and three new acylated secoiridoid glucosides from Gentiana rigescens Franch.
Fitoterapia
PUBLISHED: 07-04-2013
Show Abstract
Hide Abstract
Rigenolide A (1), a new secoiridoid glucoside with a cyclobutane skeleton and three new acylated secoiridoid glucosides, 2-(2,3-dihydroxybenzoyl)-gentiopicroside (2), 2-(2,3-dihydroxybenzoyl)-swertiamarin (3), 3-(2,3-dihydroxybenzoyl)-sweroside (4), along with two noriridoids (7 and 8) and two known secoiridoid glucosides (5 and 6), were isolated from Gentiana rigescens Franch. The structures of new compounds were elucidated by extensive spectroscopic analyses. The isolated compounds were evaluated for DPPH free-radical scavenging activity.
Related JoVE Video
Conjugates of a secoiridoid glucoside with a phenolic glucoside from the flower buds of Lonicera japonica Thunb.
Phytochemistry
PUBLISHED: 06-25-2013
Show Abstract
Hide Abstract
Secoiridoid glucosides, including two conjugates with a phenolic and two conjugates with a nicotinic acid derivative (3 and 4), together with seven known secoiridoid derivatives, were isolated from flower buds of Lonicera japonica. The structures were elucidated by spectroscopic analyses. Anti-influenza activities of six isolated compounds were also evaluated by plaque assay and neuraminidase inhibitory assay.
Related JoVE Video
Structural elucidation and biological fate of two glucuronyl metabolites of pelargonidin 3-O-?-D-glucopyranoside in rats.
J. Agric. Food Chem.
PUBLISHED: 01-09-2013
Show Abstract
Hide Abstract
A high proportion of pelargonidin 3-O-?-D-glucopyranoside (Pg3G) is metabolized to glucuronides and excreted in mammal urine after ingestion of strawberry fruit, suggesting that these metabolites play important functional roles in vivo. The aim of the present study was to elucidate the structures and determine the biological fate of the two dominant metabolites of Pg3G in rats to enable an accurate discussion of the biological properties of anthocyanins. Authentic Pg3G was orally administered to rats. One pelargonidin monoglucuronide and three Pg3G-monoglucuronides (glucuronides of the glucoside) were identified together with intact Pg3G in both blood plasma and urine samples. The structures of the two dominant metabolites were elucidated as pelargonidin 3-O-?-D-glucuronide (Pg3GlcA) and pelargonidin 3-O-?-D-glucuronyl-(1?2)-?-D-glucoside by means of (1)H and (13)C nuclear magnetic resonance spectroscopy and heteronuclear multiple-bond connective spectroscopy. The bioavailability of Pg3G in its intact form was 0.31% of the orally administered dose, and 0.65% was absorbed in the Pg3GlcA form.
Related JoVE Video
Dyscusins A-C, three new steroids from the leaves of Dysoxylum cumingianum.
Chem. Pharm. Bull.
PUBLISHED: 10-04-2011
Show Abstract
Hide Abstract
Three new steroids dyscusins A-C (1-3), including a stigmastane-type sterol and two pregnanes, together with two known steroids were isolated from the leaves of Dysoxylum cumingianum (Meliaceae). Their structures were elucidated on the basis of extensive spectroscopic analyses. In a cytotoxicity assay, compound 1 showed ten-fold enhanced cytotoxicity against multi-drug resistant cancer cells (KB-C2) in the presence of 2.5 µM colchicine as compared with the absence of colchicine. This notable finding indicated that 1 possessed a multi-drug resistant reversal effect.
Related JoVE Video
Spicachlorantins G-J, new lindenane sesquiterpenoid dimers from the roots of Chloranthus spicatus.
Chem. Pharm. Bull.
PUBLISHED: 10-04-2011
Show Abstract
Hide Abstract
Four new lindenane sesquiterpenoid dimers, spicachlorantins G-J (1-4), were isolated from the roots of Chloranthus spicatus together with seven known compounds, including chloramultilide A, shizukaol B, shizukaol D, shizukaol F, shizukaol P, chlorahololide D, and cycloshizukaol A. The planar structures of the new compounds were established by 1D-, 2D-NMR, and MS analyses. The absolute configurations of these compounds were determined by analyzing rotating Overhauser enhancement and exchange spectroscopy (ROESY) and circular dichroism (CD) spectra.
Related JoVE Video
Isolation of novel phenolic compounds with multidrug resistance (MDR) reversal properties from Onychium japonicum.
Chem. Biodivers.
PUBLISHED: 06-16-2011
Show Abstract
Hide Abstract
We isolated seven novel compounds, namely, 3,4,6-trihydroxy-2,4-dimethoxy-3-(3?,4?-dihydroxybenzyl)chalcone (1), 3,6-dihydroxy-2,4,4-trimethoxy-3-(3?,4?-dihydroxybenzyl)chalcone (2), ?,?-dihydro-3,6-dihydroxy-2,4,6-trimethoxy-3-(3?,4?-dihydroxybenzyl)chalcone (3), 3,4,4-trihydroxy-2,6-dimethoxychalcone (4), 4,5,7-trihydroxy-6-(3?,4?-dihydroxybenzyl)flavone (5), 3-(3,4-dihydroxybenzyl)-6,7-dihydroxycoumarin (6), 3-(3,4-dihydroxyphenyl)-3,4-dihydroisocoumarin (7), as well as a known compound, 3,4,7-trihydroxy-5-methoxyflavanone (8) from the whole grass of Onychium japonicum, and elucidated their structures by spectroscopic methods. Compounds 1-3 exhibited significant multidrug resistance (MDR) reversal effects on MCF-7/ADR and Bel-7402/5-Fu cell lines.
Related JoVE Video
New ?-glucosides of caffeoyl quinic acid from the leaves of Moringa oleifera Lam.
J Nat Med
PUBLISHED: 05-24-2011
Show Abstract
Hide Abstract
Two new caffeoyl quinic acid ?-glucosides, together with three known caffeoyl quinic acids and five known flavonoid glucosides, were isolated from the leaves of Moringa oleifera Lam. The structures of the new compounds were elucidated as 4-O-(4-O-?-D-glucopyranosyl)-caffeoyl quinic acid (1) and 4-O-(3-O-?-D-glucopyranosyl)-caffeoyl quinic acid (2) by spectroscopic analyses.
Related JoVE Video
Triterpenes and a triterpene glucoside from Dysoxylum cumingianum.
Phytochemistry
PUBLISHED: 04-25-2011
Show Abstract
Hide Abstract
Six triterpenes and a triterpene glucoside were isolated from the MeOH extract of the leaves of Dysoxylum cumingianum together with three known triterpenes. Their structures were elucidated by extensive spectroscopic analyses. In cytotoxicity assays against three human cancer cell lines, including a multi-drug resistant cancer cell line (KB-C2), compounds 1, 2 and 5 demonstrated significantly enhanced cytotoxicity against KB-C2 cells in the presence of 2.5 ?M colchicine, as compared with those in the absence of colchicine. This result suggested that these triterpenes might show some MDR-reversing effects.
Related JoVE Video
New 29-nor-cycloartanes with a 3,4-seco- and a novel 2,3-seco-structure from the leaves of Sinocalycanthus chinensis.
Bioorg. Med. Chem.
PUBLISHED: 02-28-2011
Show Abstract
Hide Abstract
Eight new triterpenoids, including sinocalycanchinensins A-E (1-5) with a 3,4-seco-29-nor-cycloartane skeleton, sinocalycanchinensin F (6) possessing a novel 2,3-seco-29-nor-cycloartane skeleton, and 29-nor-cycloartanes, sinocalycanchinensins G and H (7 and 8), have been isolated from the leaves of Sinocalycanthus chinensis. Their structures were elucidated on the basis of spectroscopic examinations. The cytotoxicities of the isolated new triterpenes against a panel of human cancer cell lines, including multi-drug resistant (MDR) cancer cell lines, were also evaluated. Compound 5 demonstrated enhanced cytotoxicity against MDR KB cells in the presence of colchicine, although all the compounds showed moderate or no cytotoxicities.
Related JoVE Video
A new labdanic norditerpene from Pinus sylvestris.
Nat. Prod. Res.
PUBLISHED: 10-19-2010
Show Abstract
Hide Abstract
A new labdanic norditerpene and six known labdanic diterpenes were isolated from the petroleum ether extract of the needles of Pinus sylvestris. By spectroscopic methods, the isolated compounds were determined as 15-norpinifolic acid (1), pinifolic acid (2), 4-eplimbricataloic acid (3), monomethyl pinifolate (4), 18?-acetoxylabd-8(17)-en-15-oic acid (5), 2-pentenoic acid (6) and 3?-hydroxy-8(17),E-13-labdadien-15-oic acid (7), respectively. Compound 1 showed significant anti-tumour activity.
Related JoVE Video
Conjugates of betulin derivatives with AZT as potent anti-HIV agents.
Bioorg. Med. Chem.
PUBLISHED: 06-11-2010
Show Abstract
Hide Abstract
Fourteen novel conjugates of 3,28-di-O-acylbetulins with AZT were prepared as anti-HIV agents, based on our previously reported potent anti-HIV triterpene leads, including 3-O-acyl and 3,28-di-O-acylbetulins. Nine of the conjugates (49-53, 55, 56, 59, and 60) exhibited potent anti-HIV activity at the submicromolar level, with EC(50) values ranging from 0.040 to 0.098muM in HIV-1(NL4-3) infected MT-4 cells. These compounds were equipotent or more potent than 3-O-(3,3-dimethylsuccinyl)betulinic acid (2), which is currently in Phase IIb anti-AIDS clinical trial.
Related JoVE Video
Seven new dammarane triterpenes from the floral spikes of Betula platyphylla var. japonica.
J Nat Med
PUBLISHED: 06-02-2010
Show Abstract
Hide Abstract
Seven new dammarane-type triterpenoids, including two 20(S)-hydroxy-25-methoxy-dammar-23-enes (1 and 2), two 20(S),24(R)-epoxydammaranes (3 and 4), a cabralealactone (5), and two 20(S),25-epoxydammaranes (6 and 7), together with seven known triterpenes (8-14), were isolated from the floral spikes of Betula platyphylla var. japonica. The structures for all compounds were elucidated by the analyses of extensive spectroscopic data, as well as chemical examinations.
Related JoVE Video
Immunosuppressive sesquiterpenes from Buddleja daviddi.
Planta Med.
PUBLISHED: 05-26-2010
Show Abstract
Hide Abstract
Six new sesquiterpenes, 2,6(12),10-humulatrien-7?-ol-1-one (1), 2 ?-acetoxy-5?-methoxy-enantio-caryophylla-8(15)-en-3-one (2), 2?-acetoxy-5?-hydroxy-enantio-caryophylla-8(15)-en-3-one (3), 2?-acetoxy-4?,5?-hydroxy-enantio-caryophylla-8(15)-en-3-one ( 4), 2?-acetoxy-4?,5?-hydroxy-enantio-caryophylla-8(15)-en-3-one (5), 2?-acetoxy-4-caryophyllen-8?-ol-3-one (6), and nineteen known compounds were isolated from the ethanol extract of Buddleja daviddi. The structures were elucidated by spectroscopic methods. Compounds 8-11, 14, 16, 17, and 20 showed significant immunosuppressive activities, and 8-11 and 14 were cytotoxic on HeLa and L929 cell lines.
Related JoVE Video
Petiolins J-M, prenylated acylphloroglucinols from Hypericum pseudopetiolatum var. kiusianum.
Bioorg. Med. Chem. Lett.
PUBLISHED: 05-07-2010
Show Abstract
Hide Abstract
Four new prenylated acylphloroglucinols, petiolins J-M (1-4), were isolated from aerial parts of Hypericum pseudopetiolatum var. kiusianum, and the structures were elucidated by spectroscopic data and a single-crystal X-ray diffraction analysis. Petiolin J (1) exhibited antimicrobial activity.
Related JoVE Video
Four new glucosides from the aerial parts of Mediasia macrophylla.
J Nat Med
PUBLISHED: 04-05-2010
Show Abstract
Hide Abstract
As part of our chemical studies on the medical plants in Uzbekistan aimed at searching for new drug leads, we have examined the aerial parts of Mediasia macrophylla. This has resulted in the isolation of four new glucosides, together with 30 known compounds. The structures of new compounds were elucidated as (1S)-(4-hydroxyphenyl) ethane-1,2-diol 2-O-?-glucopyranoside (1), 3-(4-methoxyphenyl)-propanol 1-O-?-glucopyranoside (2), 2-methoxy-3-hydroxy-5-(E)-propenyl-phenol 1-O-?-glucopyranoside (3), 1-O-angeloyl-?-glucopyranose (4), on the basis of spectral analysis.
Related JoVE Video
Consultation clinics for complementary and alternative medicine at Japanese university hospitals: An analysis at Tokushima University Hospital.
Exp Ther Med
PUBLISHED: 02-02-2010
Show Abstract
Hide Abstract
Here, we report on a Consultation Clinic for Complementary and Alternative Medicine (CAM) which we established at Tokushima University Hospital in July of 2007 with the aim of providing person-to-person information on CAM, though not CAM therapy itself. In December of 2008, we received 55 applications for consultation, 37% concerning health foods, 37% Japanese herbal medicine (Kampo), and 26% various other topics. The consultants (nutritionists and pharmacists) communicated individually with 38 applicants; malignancies (26%) and cardiovascular disease (24%) were the main underlying concerns. To promote the quality of consultation, data was collected by means of focus group interviews concerning the perspective of the consultants. Safe and effective use of CAM requires a network of communication linking individuals, consultation teams, physicians, primary care institutions and university hospitals. To advance this goal, we plan to broaden the efforts described herein. Our findings indicate that the specific role of the consultation clinic in promoting the scientific use of CAM merits further study.
Related JoVE Video
Papyriferic acid derivatives as reversal agents of multidrug resistance in cancer cells.
Bioorg. Med. Chem.
PUBLISHED: 01-27-2010
Show Abstract
Hide Abstract
Forty-one derivatives of papyriferic acid were prepared based on our previous finding that methyl papyriferate (3) showed potent reversing effect on cytotoxicity of colchicine against multidrug resistance (MDR) human cancer cells (KB-C2), and evaluated for their cytotoxicity and effect on reversing P-gp-mediated MDR against KB-C2 cells. 3-O-(Morpholino-beta-oxopropanoyl)-12beta-acetoxy-3alpha,25-dihydroxy-(20S,24R)-epoxydammarane (37) significantly increased the sensitivity of colchicine against KB-C2 cells by 185-fold at 5microg/mL (7.4microM), and the cytotoxicity of colchicine was recovered to nearly that of sensitive (KB) cells. The other several new amide derivatives also exhibited potent reversal activity comparable to or more potent than methyl papyriferate and verapamil.
Related JoVE Video
A C14-polyacetylenic glucoside with an alpha-pyrone moiety and four C10-polyacetylenic glucosides from Mediasia macrophylla.
Phytochemistry
PUBLISHED: 01-13-2010
Show Abstract
Hide Abstract
Polyacetylenic glucosides (1-5) were isolated from the MeOH extract of Mediasia macrophylla, and their structures were established by spectroscopic analyses. Compounds 2-4 were the first examples of C(10)-polyacetylenic glucosides found in the family Umbelliferae, while compound 1 was a unique polyacetylenic glucoside possessing an alpha-pyrone moiety.
Related JoVE Video
Petiolins F-I, benzophenone rhamnosides from Hypericum pseudopetiolatum var. kiusianum.
Chem. Pharm. Bull.
PUBLISHED: 10-06-2009
Show Abstract
Hide Abstract
Four new benzophenone-O-rhamnosides, petiolins F-I (1-4), were isolated from aerial parts of Hypericum pseudopetiolatum var. kiusianum, and the structures were elucidated by spectroscopic data and chemical means.
Related JoVE Video
Acylphloroglucinol, biyouyanagiol, biyouyanagin B, and related spiro-lactones from Hypericum chinense.
J. Nat. Prod.
PUBLISHED: 07-18-2009
Show Abstract
Hide Abstract
Biyouyanagiol (1), an acylphloroglucinol-related compound having a unique cyclopenta-1,3-dione moiety, was isolated from a Japanese traditional medicinal plant, Hypericum chinense, together with three new spiro-lactone-related derivatives, biyouyanagin B (2), 5,6-dihydrohyperolactone D (3), and 4-hydroxyhyperolactone D (4). Their structures were established on the basis of spectroscopic evidence. In a cytotoxicity assay against human cancer cell lines including multidrug-resistant (MDR) cancer cell lines, several compounds demonstrated enhanced cytotoxicity against MDR KB cells in the presence of colchicine.
Related JoVE Video
Cytotoxic triterpene saponins from Alternanthera philoxeroides.
J Asian Nat Prod Res
PUBLISHED: 05-02-2009
Show Abstract
Hide Abstract
Four new pentacyclic triterpene saponins, philoxeroidesides A-D (1-4) were isolated from the aerial parts of Alternanthera philoxeroides. Their structures were elucidated on the basis of 1D- and 2D-NMR experiments and MS analyses. Philoxeroidesides A-D (1-4) showed cytotoxic activities against SK-N-SH and HL60 cell lines.
Related JoVE Video
Triterpenes from Astilbe chinensis.
J Asian Nat Prod Res
PUBLISHED: 05-02-2009
Show Abstract
Hide Abstract
Six new triterpenes, 3beta,6beta-dihydroxyurs-12-en-27-oic acid (1), 3beta,6beta,24-trihydroxyurs-12-en-27-oic acid (2), 3beta,6beta,7alpha-trihydroxyurs-12-en-27-oic acid (3), 3beta-acetoxy-6beta-hydroxyurs-12-en-27-oic acid (4), 3beta,6beta,24-trihydroxyolean-12-en-27-oic acid (5), and 3beta,6beta,7alpha-trihydroxyolean-12-en-27-oic acid (6), were isolated from the rhizomes of Astilbe chinensis. Their structures were elucidated on the basis of 1D- and 2D-NMR analyses and HR-MS experiments. The isolated compounds exhibited significant cytotoxic activities against the SK-N-SH and HL-60 cell lines.
Related JoVE Video
Xanthones from Hypericum chinense and their cytotoxicity evaluation.
Phytochemistry
PUBLISHED: 03-06-2009
Show Abstract
Hide Abstract
A xanthonolignoid, 2-O-demethylkielcorin, and a phenylxanthone, chinexanthone A, were isolated from stems of Hypericum chinense together with four known xanthonolignoids and seven known xanthones. Their structures were established by spectroscopic analysis, as their optical properties and absolute stereochemistry determined. The cytotoxicities of the isolated xanthone derivatives as well as additional 32 xanthones against a panel of human cancer cell lines were also evaluated.
Related JoVE Video
Chromone and chromanone glucosides from Hypericum sikokumontanum and their anti-Helicobacter pylori activities.
Phytochemistry
PUBLISHED: 01-06-2009
Show Abstract
Hide Abstract
Chromone glucosides, takanechromones A-C (1, 2 and 5) and chromanone glucosides, named takanechromanones A and B (3 and 4), were isolated from the methanolic extracts of Hypericumsikokumontanum together with 27 known compounds. Their structures were established based on spectroscopic evidence. The isolated compounds and some chromone derivatives were assayed for antimicrobial activity against Helicobacter pylori and cytotoxicity against human cancer cell lines.
Related JoVE Video
Prenylated flavonoids from the roots of Desmodium caudatum and evaluation of their antifungal activity.
Planta Med.
Show Abstract
Hide Abstract
Two new prenylated flavonoids (1, 4) and two new prenylated C-methyl-flavonoids (6, 7), together with four known flavonoids (2, 3, 5, 8), were isolated from the roots of Desmodium caudatum. The structures of the new compounds were elucidated by extensive spectroscopic analyses including 1D-, 2D-NMR and MS. The antifungal activities of five compounds (1, 2, 4, 6, 8) as well as nine flavonoids (9-17) previously isolated from this plant against Aspergillus niger, Penicillium sp., Rhizopus sp., and Trichophyton sp. were evaluated. Compound 6 showed potent antifungal activity against Trichophyton sp. with a minimum inhibitory concentration (MIC) value of 1.95?µg/mL.
Related JoVE Video
Prenylated flavonoids from Desmodium caudatum and evaluation of their anti-MRSA activity.
Phytochemistry
Show Abstract
Hide Abstract
Seven prenylated flavonoids and a prenylated chromanochroman derivative, together with eight known flavonoids, were isolated from roots of Desmodium caudatum. The 15 structures were elucidated by extensive spectroscopic analyses. The antibacterial activity of many of other compounds was evaluated against methicillin-resistant Staphylococcus aureus (MRSA: COL and 5) by a disc diffusion method, and the minimum inhibitory concentrations (MICs) to MRSA were determined.
Related JoVE Video
Sesquiterpene lactone glycosides from the roots of Ferula varia.
Chem. Pharm. Bull.
Show Abstract
Hide Abstract
Seven new sesquiterpene lactone glycosides (1-7) were isolated from the H2O-soluble fraction from the MeOH extract of the roots of Ferula varia. Their structures were elucidated by extensive spectroscopic analyses. The absolute configurations of compounds 1 and 2 were determined by modified Moshers method.
Related JoVE Video
Lignans from Schisandra propinqua with inhibitory effects on lymphocyte proliferation.
Planta Med.
Show Abstract
Hide Abstract
Seven new dibenzocyclooctadiene lignans, named propinquain E-K, together with 11 known lignans were isolated from the ethanol extract of the roots of Schisandra propinqua subsp. sinensis. The structures of the new compounds were elucidated by spectroscopic methods, including ¹H- and ¹³C-NMR, 2D-NMR, HR ESI-MS, and CD spectra. Two dibenzocyclooctadienes revealed inhibitory effects on lymphocyte proliferation.
Related JoVE Video
Quantification of phosphatidic acid in foodstuffs using a thin-layer-chromatography-imaging technique.
J. Agric. Food Chem.
Show Abstract
Hide Abstract
Apical application of lysophosphatidic acid (LPA), a growth-factor-like phospholipid, was shown to prevent or restore gastrointestinal (GI) disorders, such as diarrhea and stomach ulcer, in experimental animals. Because LPA is formed from phosphatidic acid (PA) by the activity of digestive phospholipase A(2), PA is a potential component for dietary treatment of such GI disorders. Here, we quantified PA contained in 38 foodstuffs and 3 herbs by a thin-layer-chromatography-imaging technique. Vegetables belonging to Brassicaceae, such as cabbage leaves (700 nmol/g of wet weight) and Japanese radish leaves (570 nmol/g), contained higher amounts of PA than other foodstuffs. Amounts of PA in fruits, cereals, and starchy root vegetables were below 300 nmol/g. Animal foodstuffs contained low amounts of PA (<60 nmol/g). Interestingly, leaves of Mallotus japonicas, a Japanese edible herb used for treatment of stomach ulcer, had the highest PA (1410 nmol/g) among those examined. The data shown here will be useful for the development of dietary treatment for a damaged GI tract.
Related JoVE Video

What is Visualize?

JoVE Visualize is a tool created to match the last 5 years of PubMed publications to methods in JoVE's video library.

How does it work?

We use abstracts found on PubMed and match them to JoVE videos to create a list of 10 to 30 related methods videos.

Video X seems to be unrelated to Abstract Y...

In developing our video relationships, we compare around 5 million PubMed articles to our library of over 4,500 methods videos. In some cases the language used in the PubMed abstracts makes matching that content to a JoVE video difficult. In other cases, there happens not to be any content in our video library that is relevant to the topic of a given abstract. In these cases, our algorithms are trying their best to display videos with relevant content, which can sometimes result in matched videos with only a slight relation.