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Find video protocols related to scientific articles indexed in Pubmed.
Influence of Recombinant Lentiviral Vector Encoding miR-15a/16-1 in Biological Features of Human Nasopharyngeal Carcinoma CNE-2Z Cells.
Cancer Biother. Radiopharm.
PUBLISHED: 11-19-2014
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Abstract MicroRNAs can function as tumor suppressor miRNAs. Bcl-2 is an antiapoptotic gene overexpressed in many tumors, including nasopharyngeal carcinoma (NPC). It is reported that microRNA-15a (miR-15a) and microRNA-16-1 (miR-16-1) could act as bcl-2 inhibitors. To investigate their effects on NPC, the authors used recombinant lentiviral vector to upregulate the expression of miR-15a/16-1 in NPC CNE-2Z cells. The authors divided cells into the control group, transfection group, radiotherapy group, and transfection-radiotherapy group. In this experiment, reverse transcription-quantitative polymerase chain reaction was used to detect the expression of miR-15a/16-1 and bcl-2 mRNA. Cell proliferation was analyzed by MTT assay. Flow cytometry was used to measure cell apoptosis. Radiosensitivity was measured using colony-forming experiment. The protein expression of bcl-2 was measured by western blot, the activation levels of caspase were detected by a spectrophotometric method. After transfection, cell proliferation was inhibited, while the apoptosis rate and radiosensitivity were increased. In addition, the activation of caspase-2 and caspase-3 was aggrandized correspondingly. Although the expression levels of bcl-2 mRNA in each group had no difference, the protein expression of bcl-2 was downregulated. These results suggested that miR-15a/16-1 could inhibit cell proliferation and increase the apoptosis and radiosensitivity of CNE-2 cells, by regulating the bcl-2 gene at post-transcriptional level and by increasing the activation of caspase-2 and caspase-3.
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Efficient cycloaddition of epoxides and carbon dioxide over novel organic-inorganic hybrid zeolite catalysts.
Chem. Commun. (Camb.)
PUBLISHED: 11-06-2014
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Organic-inorganic hybrid zeolites with the MFI-type lamellar structure serve as efficient solid Lewis base catalysts for solvent-free synthesis of a variety of cyclic carbonates from corresponding epoxides and carbon dioxide. The ion-exchange with iodide, in particular, renders these materials an excellent catalytic activity and good recyclability.
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Poultry Market Closures and Human Infection with Influenza A(H7N9) Virus, China, 2013-14.
Emerging Infect. Dis.
PUBLISHED: 10-24-2014
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Closure of live poultry markets was implemented in areas affected by the influenza virus A(H7N9) outbreak in China during winter, 2013-14. Our analysis showed that closing live poultry markets in the most affected cities of Guangdong and Zhejiang provinces was highly effective in reducing the risk for H7N9 infection in humans.
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Low-toxic Mn-doped ZnSe@ZnS quantum dots conjugated with nano-hydroxyapatite for cell imaging.
Nanoscale
PUBLISHED: 10-18-2014
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Fluorescent bio-imaging has received significant attention in a myriad of research disciplines, and QDs are playing an increasingly important role in these areas. Doped QDs, an important alternative to conventional heavy metal-containing QDs are employed for biomedical applications. However, since QDs are exogenous substances to the biological environment, the biocompatibility of QDs is expected to be challenging in some cases. Herein, nano fluorine-doped hydroxyapatite (FAp, a well-known biocompatible material) was introduced to endow biocompatibility to Cd-free Mn-doped ZnSe@ZnS QDs. Thus, a nano-FAp-QD conjugate was developed and the biocompatibility, as well as potential cell imaging application, was investigated. To construct the proposed conjugate, Cd-free highly luminescent Mn-doped ZnSe@ZnS QDs and monodispersed nano-FAp were first prepared in high-temperature organic media. For facilitating the conjugation, hydrophobic nano-FAp was made water soluble via o-phosphoethanolamine (PEA) coating, which further provides conjugating sites for QDs to anchor. Cytotoxicity studies indicated the developed conjugate indeed possesses good compatibility and low toxicity to cells. The nano-FAp-QDs conjugate was successfully employed for cancer cell staining for at least 24 h, demonstrating the potential usefulness of this material in future biomedical research.
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Analyte-Activable Probe for Protease Based on Cytochrome C-Capped Mn: ZnS Quantum Dots.
Anal. Chem.
PUBLISHED: 10-06-2014
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A new sensor format was proposed here by integrating conjugation of analyte-recognition sites and quenching the luminescence of quantum dots (QDs) in one pot during the synthesis of QDs, with protease as the model analyte. Inherently phosphorescence-attenuated Mn-doped ZnS QDs were prepared with electron transfer protein cytochrome C (Cyt C) as the ligand, which was capable of protease sensing in both label-free and activable format. This detection strategy eliminates the postsynthetic protein conjugation and responses to analyte in the turn-on mode, lowering the signal background. In the presence of protease, the initially "locked" phosphorescence of Mn-doped ZnS QDs could be activated, due to the enzymatic digestion of surface Cyt C ligand and removal of the electron-transfer quenching unit away from the close-proximity of QDs. The proposed probe exhibited good selectivity toward proteases over other proteins and enzymes. Besides, it was also capable of differentiating active and inactive serine proteases. Analytical performance of this probe was evaluated using trypsin as the model serine protease. Limits of detection (LOD) of 2 nM was obtained, which is well below the average urine trypsin level of patients. The analytical application of this probe was demonstrated in determination of trypsin in human pancreatic carcinoma (PANC-1 and 818.4) cells lysates, demonstrating the potential usefulness of this probe in future clinical diagnosis.
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Down-regulated MYH11 expression correlates with poor prognosis in stage II and III colorectal cancer.
Asian Pac. J. Cancer Prev.
PUBLISHED: 09-18-2014
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The MYH11 gene may be related to cell migration and adhesion, intracellular transport, and signal transduction. However, its relationship with prognosis is still uncertain. The aim of this study was to investigate correlations between MYH11 gene expression and prognosis in 58 patients with stage II and III colorectal cancer. Quantitative real-time polymerase chain reaction was performed in fresh CRC tissues to examine mRNA expression, and immunohistochemistry was performed with paraffin-embedded specimens for protein expression. On univariate analysis, MYH11 expression at both mRNA and protein levels, perineural invasion and lymphovascular invasion were related to disease-free survival (p<0.05; log-rank test). Cancers with lower MYH11 expression were more likely to have a poor prognosis. Otherwise, MYH11 expression was unrelated to patient clinicopathological features. On multivariate analysis, low MYH11 expression proved to be an independent adverse prognosticator (p<0.05). These findings show that MYH11 can contribute to predicting prognosis in stage II and III colorectal cancers.
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[Research on glucose measuring technique by surface plasmon resonance based on thiol coupling].
Guang Pu Xue Yu Guang Pu Fen Xi
PUBLISHED: 09-12-2014
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In the glucose measuring technique by surface plasmon resonance, D-galactose/D-glucose binding protein (GGBP) that can specifically adsorb glucose was introduced, and high-precision specific detection of glucose concentration was realized. In the present paper, the GGBP protein was bound on the surface of SPR sensor through thiol coupling method. GGBP binding experiment was carried out on SPR sensor and then glucose concentration experiment was conducted with this sensor. The results indicated that the SPR sensor had good linearity, stability and repeatability in the range of 0.1-10 mg x dL(-1). SPR sensor bound with GGBP would have great potential and vast development prospects.
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Genetic analysis of amino acid content in wheat grain.
J. Genet.
PUBLISHED: 09-06-2014
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Complete diallel crosses with five parents of common wheat (Triticum aestivum L.) were conducted to analyse inheritance of 17 amino acid contents by using the genetic model including seed, cytoplasmic, maternal and environment interaction effects on quantitative traits of seeds in cereal crops. The results showed that inheritance of 17 amino acid contents, except tyrosine, was controlled by several genetic systems including seed, cytoplasmic, and maternal effects, and by significant gene x environment interaction effects. Seed-direct additive and maternal effects constituted a major part of genetic effects for lysine, tyrosine, arginine, methionine, and glutamic acid content. Seed-direct additive effect formed main part in inheritance of isoleucine and serine contents. Threonine content was mainly governed by maternal additive effect. The other nine amino acid contents were almost entirely controlled by dominance effects. High general heritability of tyrosine (36.3%), arginine (45.8%), lysine (24.7%) and threonine (21.4%) contents, revealed that it could be effective to improve them by direct selection in progenies from appropriate crosses. Interaction heritability for phenylalanine, proline, and histidine content, which was 36.1%, 39.5% and 25.7%, respectively, was higher than for the other amino acids.
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Use of laser speckle contrast imaging to reveal changes in temperature and blood perfusion in the skin of healthy subjects after administration of heated moxa sticks and daiwenjiu ointment.
J Tradit Chin Med
PUBLISHED: 09-05-2014
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To investigate the influence of heated moxa sticks ("moxibustion") and Daiwenjiu ointment (DO) on changes in temperature and blood perfusion volume on the skin of the backs of healthy subjects.
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In situ activation of CdS electrochemiluminescence film and its application in H?S detection.
Anal. Chem.
PUBLISHED: 08-15-2014
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Nanocrystals (NCs) usually suffer from weak electrogenerated chemiluminescence (ECL) emissions compared with conventional luminescent reagents like Ru(bpy)3(2+). In this work, we proposed a simple in situ activation approach by dipping CdS NCs film on glass carbon electrode (CdS NCs/GCE) in an activation solution containing H2O2 and citric acid, resulting in a ~58-fold enhancement of ECL intensity in the presence of coreactant H2O2. During activation, CdS NCs were oxidized by H2O2 to smaller ones which resulted in more surface S vacancies; meanwhile, citric acid played an important role in stabilizing NCs. The ECL enhancing mechanism was investigated in detail, and the coordination of H2O2 to surface excess Cd(2+) ions (S vacancies) on the CdS NCs surface formed in activation was the main factor which could stabilize the electrogenerated radicals, resulting in an enhanced ECL. ECL from the activated CdS NCs/GCE could be quenched in Na2S solution due to the bonding of S(II) to excess Cd(2+) ions on the surface of CdS NCs. On the basis of this, we then used the activated CdS NCs/GCE as an ECL probe for the detection of Na2S which showed good performance including a wide linear range of 5 nM to 20 ?M and good anti-interference ability. Moreover, this ECL probe was successfully applied for hydrogen sulfide (H2S) detection in a biological system.
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Hyperfibrinogenemia in appendicitis: a new predictor of perforation in children.
Pediatr. Surg. Int.
PUBLISHED: 08-13-2014
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Acute appendicitis is the most common emergency abdominal inflammation requiring operation in children. As an acute-phase protein, plasma fibrinogen always increases with inflammation or tissue necrosis. This had brought about the assumption that hyperfibrinogenemia in patients with appendicitis may have a predictive ability for the preoperative diagnosis of appendiceal. Aim of this retrospective study was to assess the diagnostic value of hyperfibrinogenemia as a preoperative laboratory marker for appendiceal perforation in children with acute appendicitis.
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De novo transcriptome sequencing and digital gene expression analysis predict biosynthetic pathway of rhynchophylline and isorhynchophylline from Uncaria rhynchophylla, a non-model plant with potent anti-alzheimer's properties.
BMC Genomics
PUBLISHED: 08-12-2014
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The major medicinal alkaloids isolated from Uncaria rhynchophylla (gouteng in chinese) capsules are rhynchophylline (RIN) and isorhynchophylline (IRN). Extracts containing these terpene indole alkaloids (TIAs) can inhibit the formation and destabilize preformed fibrils of amyloid ? protein (a pathological marker of Alzheimer's disease), and have been shown to improve the cognitive function of mice with Alzheimer-like symptoms. The biosynthetic pathways of RIN and IRN are largely unknown.
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CHoMP: A Chemoenzymatic Histology Method Using Clickable Probes.
Chembiochem
PUBLISHED: 07-31-2014
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The characterization of aberrant glycosylation patterns in biopsied patient samples represents a remarkable challenge for scientists and medical doctors due to the lack of specific methods for detection. Here, we report the development of a histological method, dubbed CHoMP-chemoenzymatic histology of membrane polysaccharides-for analyzing glycosylation patterns in mammalian tissues. This method exploits a recombinant glycosyltransferase to transfer a monosaccharide analogue equipped with a chemical handle to a specific cell-surface glycan target, which can then be derivatized with imaging probes by using bioorthogonal click chemistry for visualization. We applied CHoMP to survey changes in expression of N-acetyllactosamine (LacNAc) in human samples from patients afflicted with lung adenocarcinoma and observed a sharp decrease in expression levels between normal and early grade tumors, thus suggesting a potential application of this technique in early cancer diagnosis.
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Human exposure to live poultry and psychological and behavioral responses to influenza A(H7N9), China.
Emerging Infect. Dis.
PUBLISHED: 07-31-2014
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To investigate human exposure to live poultry and changes in risk perception and behavior after the April 2013 influenza A(H7N9) outbreak in China, we surveyed 2,504 urban residents in 5 cities and 1,227 rural residents in 4 provinces and found that perceived risk for influenza A(H7N9) was low. The highest rate of exposure to live poultry was reported in Guangzhou, where 47% of those surveyed reported visiting a live poultry market > or =1 times in the previous year. Most (77%) urban respondents reported that they visited live markets less often after influenza A(H7N9) cases were first identified in China in March 2013, but only 30% supported permanent closure of the markets to control the epidemic. In rural areas, 48% of respondents reported that they raised backyard poultry. Exposure to live commercial and private poultry is common in urban and rural China and remains a potential risk factor for human infection with novel influenza viruses.
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Negative feedback regulation of Wnt signaling via N-linked fucosylation in zebrafish.
Dev. Biol.
PUBLISHED: 07-25-2014
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L-fucose, a monosaccharide widely distributed in eukaryotes and certain bacteria, is a determinant of many functional glycans that play central roles in numerous biological processes. The molecular mechanism, however, by which fucosylation mediates these processes remains largely elusive. To study how changes in fucosylation impact embryonic development, we up-regulated N-linked fucosylation via over-expression of a key GDP-Fucose transporter, Slc35c1, in zebrafish. We show that Slc35c1 overexpression causes elevated N-linked fucosylation and disrupts embryonic patterning in a transporter activity dependent manner. We demonstrate that patterning defects associated with enhanced N-linked fucosylation are due to diminished canonical Wnt signaling. Chimeric analyses demonstrate that elevated Slc35c1 expression in receiving cells decreases the signaling range of Wnt8a during zebrafish embryogenesis. Moreover, we provide biochemical evidence that this decrease is associated with reduced Wnt8 ligand and elevated Lrp6 coreceptor, which we show are both substrates for N-linked fucosylation in zebrafish embryos. Strikingly, slc35c1 expression is regulated by canonical Wnt signaling. These results suggest that Wnt limits its own signaling activity in part via up-regulation of a transporter, slc35c1 that promotes terminal fucosylation and thereby limits Wnt activity.
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Gender-Specific Risk Factors for Virologic Failure in KwaZulu-Natal: Automobile Ownership and Financial Insecurity.
AIDS Behav
PUBLISHED: 07-20-2014
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We sought to examine which socioeconomic indicators are risk factors for virologic failure among HIV-1 infected patients receiving antiretroviral therapy (ART) in KwaZulu-Natal, South Africa. A case-control study of virologic failure was conducted among patients recruited from the outpatient clinic at McCord Hospital in Durban, South Africa between October 1, 2010 and June 30, 2012. Cases were those failing first-line ART, defined as viral load >1,000 copies/mL. Univariate logistic regression was performed on sociodemographic data for the outcome of virologic failure. Variables found significant (p < 0.05) were used in multivariate models and all models were stratified by gender. Of 158 cases and 300 controls, 35 % were male and median age was 40 years. Gender stratification of models revealed automobile ownership was a risk factor among males, while variables of financial insecurity (unemployment, non-spouse family paying for care, staying with family) were risk factors for women. In this cohort, financial insecurity among women and automobile ownership among men were risk factors for virologic failure. Risk factor differences between genders demonstrate limitations of generalized risk factor analysis.
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Long-term ketamine abuse induces cystitis in rats by impairing the bladder epithelial barrier.
Mol. Biol. Rep.
PUBLISHED: 07-16-2014
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Long-term ketamine abuse is known to affect the lower urinary tract and produce symptoms of cystitis. However, the pathophysiology and causative mechanism of the changes in bladder function remain unclear. The present study aimed to investigate the existence of ketamine-induced cystitis in a rat model and characterize the underlining mechanisms. Rats were assigned to blank control, normal saline (NS), low-dose ketamine (LK, 5 mg/kg), and high-dose ketamine (HK, 50 mg/kg) groups. The two experimental groups received ketamine hydrochloride daily for 16 weeks. All rats were housed individually for assessment of urinary frequency and urine volume. Urinary biomarkers were measured at different time points. Rat bladders were excised for histopathology, immunohistochemistry, and western blot analysis. Ketamine-treated rats had increased urinary frequency compared to NS-treated rats at Week 16. Urinary nitric oxide and antiproliferative factor levels were increased in ketamine-treated rats within the first 30 h after administration. After long-term ketamine administration, urinary glycoprotein GP51 and potassium levels were decreased in the HK and LK groups compared to the NS group. Ketamine-treated rats showed thickened bladder epithelial layer, increased expression of inducible nitric oxide synthase and occludin, and decreased expression of zonula occludens-1 in the bladder wall. Ketamine, or its urinary metabolites, disrupted the proliferation of bladder epithelial cells, resulting in defected bladder epithelial barrier. Subsequent leakage of urinary potassium causes a stress response in the bladder and provokes cystitis.
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Association of the 3'UTR FOXO3a polymorphism rs4946936 with an increased risk of childhood acute lymphoblastic leukemia in a Chinese population.
Cell. Physiol. Biochem.
PUBLISHED: 06-04-2014
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FOXO3a is an essential tumor suppressor that regulates the mechanisms of tumorigenesis and leukemogenesis. FOXO3a polymorphisms have not been reported previously associated with an increased risk for childhood acute lymphoblastic leukemia (ALL). In this study, the rs4946936 polymorphism located in the 3'UTR of FOXO3a was selected to evaluate its relationship with a risk for ALL in Chinese children.
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Biocompatible click chemistry enabled compartment-specific pH measurement inside E. coli.
Nat Commun
PUBLISHED: 06-01-2014
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Bioorthogonal reactions, especially the Cu(I)-catalysed azide-alkyne cycloaddition, have revolutionized our ability to label and manipulate biomolecules under living conditions. The cytotoxicity of Cu(I) ions, however, has hindered the application of this reaction in the internal space of living cells. By systematically surveying a panel of Cu(I)-stabilizing ligands in promoting protein labelling within the cytoplasm of Escherichia coli, we identify a highly efficient and biocompatible catalyst for intracellular modification of proteins by azide-alkyne cycloaddition. This reaction permits us to conjugate an environment-sensitive fluorophore site specifically onto HdeA, an acid-stress chaperone that adopts pH-dependent conformational changes, in both the periplasm and cytoplasm of E. coli. The resulting protein-fluorophore hybrid pH indicators enable compartment-specific pH measurement to determine the pH gradient across the E. coli cytoplasmic membrane. This construct also allows the measurement of E. coli transmembrane potential, and the determination of the proton motive force across its inner membrane under normal and acid-stress conditions.
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Surgical and conservative treatments of complete spontaneous posterior interosseous nerve palsy with hourglass-like fascicular constrictions: a retrospective study of 41 cases.
Neurosurgery
PUBLISHED: 05-29-2014
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The surgical treatment of spontaneous posterior interosseous nerve (PIN) palsy with hourglass-like fascicular constriction (HLFC) remains controversial.
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Potential biomarkers and latent pathways for vasculitis based on latent pathway identification analysis.
Int J Rheum Dis
PUBLISHED: 05-27-2014
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We aimed in this study to identify the significant latent pathways and precise molecular mechanisms underlying the syndrome of vasculitis.
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[Injury characteristics and surgical treatment of lower lumbar vertebral burst fractures].
Zhongguo Gu Shang
PUBLISHED: 05-16-2014
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To investigate the feasibility of utilizing self-designed score system for lower lumbar vertebral burst fractures to select surgical approach.
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Increasing prevalence of extended-spectrum cephalosporin-resistant Escherichia coli in food animals and the diversity of CTX-M genotypes during 2003-2012.
Vet. Microbiol.
PUBLISHED: 04-30-2014
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The aim of this study was to investigate the trends and the diversity of CTX-M types of extended-spectrum ?-lactamase (ESBL) in Escherichia coli isolated from food animals in China over a ten-year period. From 2003 to 2012, 2815 E. coli isolates collected from diseased animals (chickens, pigs, and waterfowl) were screened for the prevalence of CTX-M genes. CTX-M-positive isolates were tested for their susceptibilities to 10 antimicrobial agents and the clonal relationship of CTX-M-producing E. coli isolates was also assessed. Overall, 677 (20.1%) of the 2815 E. coli isolates carried CTX-M genes. Eighteen different types of CTX-M ESBLs were identified, with CTX-M-14, CTX-M-55, and CTX-M-65 being the most dominant genotypes. The occurrence of CTX-M-producing E. coli increased significantly from 5.7% in 2003-2005 to 35.3% in 2009-2012 (p<0.0001). High genetic heterogeneities were observed in the CTX-M-producing E. coli isolates. Most CTX-M-producing strains were also resistant to other classes of antimicrobials. Compared to isolates carrying CTX-M-9 subgroup of ESBLs, isolates carrying CTX-M-1 subgroup ESBLs showed significantly higher resistance rates to ceftazidime, amikacin, and fosfomycin (p<0.01). The study reported the dramatic increase of CTX-M ESBLs in E. coli isolated from animals overtime in China. The increasing incidence of CTX-M-55 with high hydrolytic activity against ceftazidime and the widely spread co-resistance in CTX-M-producing isolates alarm the serious antimicrobial resistance situation in China and highlight the need for urgent control strategies to limit the dissemination of those resistant genes in China.
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Responses to threat of influenza A(H7N9) and support for live poultry markets, Hong Kong, 2013.
Emerging Infect. Dis.
PUBLISHED: 04-23-2014
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We conducted a population survey in Hong Kong to gauge psychological and behavioral responses to the threat of influenza A(H7N9) and support for closure of live poultry markets. We found low anxiety and low levels of exposure to live poultry but mixed support for permanent closure of the markets.
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Discovery of novel genes and gene isoforms by integrating transcriptomic and proteomic profiling from mouse liver.
J. Proteome Res.
PUBLISHED: 04-18-2014
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Comprehensively identifying gene expression in both transcriptomic and proteomic levels of one tissue is a prerequisite for a deeper understanding of its biological functions. Alternative splicing and RNA editing, two main forms of transcriptional processing, play important roles in transcriptome and proteome diversity and result in multiple isoforms for one gene, which are hard to identify by mass spectrometry (MS)-based proteomics approach due to the relative lack of isoform information in standard protein databases. In our study, we employed MS and RNA-Seq in parallel into mouse liver tissue and captured a considerable catalogue of both transcripts and proteins that, respectively, covered 60 and 34% of protein-coding genes in Ensembl. We then developed a bioinformatics workflow for building a customized protein database that for the first time included new splicing-derived peptides and RNA-editing-caused peptide variants, allowing us to more completely identify protein isoforms. Using this experimentally determined database, we totally identified 150 peptides not present in standard biological databases at false discovery rate of <1%, corresponding to 72 novel splicing isoforms, 43 new genetic regions, and 15 RNA-editing sites. Of these, 11 randomly selected novel events passed experimental verification by PCR and Sanger sequencing. New discoveries of gene products with high confidence in two omics levels demonstrated the robustness and effectiveness of our approach and its potential application into improve genome annotation. All the MS data have been deposited to the iProx ( http://ww.iprox.org ) with the identifier IPX00003601.
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Higher FOXP3-TSDR demethylation rates in adjacent normal tissues in patients with colon cancer were associated with worse survival.
Mol. Cancer
PUBLISHED: 04-06-2014
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The influence of natural regulatory T cells (nTregs) on the patients with colon cancer is unclear. Demethylated status of the Treg-specific demethylated region (TSDR) of the FOXP3 gene was reported to be a potential biomarker for the identification of nTregs.
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Structural diversity of lamellar zeolite Nu-6(1)--postsynthesis of delaminated analogues.
Dalton Trans
PUBLISHED: 03-21-2014
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Nu-6(1) zeolite, the lamellar precursor of NSI topology, was firstly synthesized with 4'4-bipyridine as the structure-directing agent (SDA) and then subjected to HCl-EtOH treatment for the purpose of structural modification. Interlayer deconstruction and reconstruction took place alternately in this acid treatment. An intermediate named ECNU-4 was separated at the initial stage of this continuous treatment process, which exhibited a special X-ray diffraction pattern without obvious reflection peaks at low angles. The zeolitic structure in the intralayer sheets was supposed to be well preserved in ECNU-4, whereas the interlayer structure became extremely disordered. The ECNU-4 intermediate showed structural diversity. It was converted into the reconstructed and interlayer expanded zeolite IEZ-NSI without an external silicon source by prolonging the HCl-EtOH treatment to 24 h. Moreover, with a partially delaminated structure, ECNU-4 was easily interlayer swollen at room temperature with cetyltrimethyl ammonium bromide in the presence of tetrapropyl ammonium hydroxide. The swollen material was further sonicated to yield a more deeply delaminated zeolite, Del-Nu-6. ECNU-4 and Del-Nu-6 differed in delamination degree, structural disordering and textural properties, especially surface area.
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SILAC-based quantitative proteomic analysis of secretome between activated and reverted hepatic stellate cells.
Proteomics
PUBLISHED: 03-14-2014
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Activated hepatic stellate cell (HSC) is the main myofibroblast cell in the liver fibrosis (LF). An important characteristic of the recovery of LF is not only the apoptosis of activated HSCs but also reversal of myofibroblast-like phenotype to a quiescent-like phenotype. Understanding the changes of secreted proteins in the reversion of activated HSCs may provide the broader view of cellular regulatory networks and discover candidate markers or targets for therapeutic strategies of LF. In this study, stable isotope labeling with amino acids (SILAC) combined with linear ion trap-Fourier transform ion cyclotron resonance mass spectrometer (LTQ-FT MS) was performed on in vitro activated HSCs and reverted HSCs to obtain a proteomic view of secretory proteins. In total, 330 proteins showed significant differences in reverted HSCs. Among these, 109 upregulated proteins were mainly involved in amino acid metabolism pathway and glucose metabolism pathway using GeneGO/MetaCore software, while 221 downregulated proteins are closely associated with HSCs activation, such as cytoskeleton remodeling, chemokines, and cell adhesion. Additionally, a set of novel proteins associated with HSCs activation and reversion were validated by Western blotting in the cell secretion and in the sera of LF, including vitronectin, laminin beta 1, and ubiquitin conjugation factor E4B. Our study provided the valuable insight into the mechanisms in the reversion of activated HSCs and identified some potential biomarkers of LF in clinical studies. All MS data have been deposited in the ProteomeXchange with identifier PXD000773 (http://proteomecentral.proteomexchange.org/dataset/PXD000773).
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Fast imaging of eccrine latent fingerprints with nontoxic Mn-doped ZnS QDs.
Anal. Chem.
PUBLISHED: 03-14-2014
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Fingerprints are unique characteristics of an individual, and their imaging and recognition is a top-priority task in forensic science. Fast LFP (latent fingerprint) acquirement can greatly help policemen in screening the potential criminal scenes and capturing fingerprint clues. Of the two major latent fingerprints (LFP), eccrine is expected to be more representative than sebaceous in LFP identification. Here we explored the heavy metal-free Mn-doped ZnS quantum dots (QDs) as a new imaging moiety for eccrine LFPs. To study the effects of different ligands on the LFP image quality, we prepared Mn-doped ZnS QDs with various surface-capping ligands using QDs synthesized in high-temperature organic media as starting material. The orange fluorescence emission from Mn-doped ZnS QDs clearly revealed the optical images of eccrine LFPs. Interestingly, N-acetyl-cysteine-capped Mn-doped ZnS QDs could stain the eccrine LFPs in as fast as 5 s. Meanwhile, the levels 2 and 3 substructures of the fingerprints could also be simultaneously and clearly identified. While in the absence of QDs or without rubbing and stamping the finger onto foil, no fluorescent fingerprint images could be visualized. Besides fresh fingerprint, aged (5, 10, and 50 days), incomplete eccrine LFPs could also be successfully stained with N-acetyl-cysteine-capped Mn-doped ZnS QDs, demonstrating the analytical potential of this method in real world applications. The method was also robust for imaging of eccrine LFPs on a series of nonporous surfaces, such as aluminum foil, compact discs, glass, and black plastic bags.
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Monitoring dynamic glycosylation in vivo using supersensitive click chemistry.
Bioconjug. Chem.
PUBLISHED: 03-14-2014
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To monitor the kinetics of biological processes that take place within the minute time scale, simple and fast analytical methods are required. In this article, we present our discovery of an azide with an internal Cu(I)-chelating motif that enabled the development of the fastest protocol for Cu(I)-catalyzed azide-alkyne cycloaddition (CuAAC) to date, and its application toward following the dynamic process of glycan biosynthesis. We discovered that an electron-donating picolyl azide boosted the efficiency of the ligand-accelerated CuAAC 20-38-fold in living systems with no apparent toxicity. With a combination of this azide and BTTPS, a tris(triazolylmethyl)amine-based ligand for Cu(I), we were able to detect newly synthesized cell-surface glycans by flow cytometry using as low as 1 nM of a metabolic precursor. This supersensitive chemistry enabled us to monitor the dynamic glycan biosynthesis in mammalian cells and in early zebrafish embryogenesis. In live mammalian cells, we discovered that it takes approximately 30-45 min for a monosaccharide building block to be metabolized and incorporated into cell-surface glycoconjugates. In zebrafish embryos, the labeled glycans could be detected as early as the two-cell stage. To our knowledge, this was the first time that newly synthesized glycans were detected at the cleavage period (0.75-2 hpf) in an animal model using bioorthogonal chemistry.
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A clinical prediction rule for diagnosing human infections with avian influenza A(H7N9) in a hospital emergency department setting.
BMC Med
PUBLISHED: 02-20-2014
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Human infections with avian influenza A(H7N9) virus are associated with severe illness and high mortality. To better inform triage decisions of hospitalization and management, we developed a clinical prediction rule for diagnosing patients with A(H7N9) and determined its predictive performance.
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Transcutaneous vagus nerve stimulation for refractory epilepsy: a randomized controlled trial.
Clin. Sci.
PUBLISHED: 02-13-2014
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This trial was designed to test the safety and effectiveness of transcutaneous auricular vagus nerve stimulation (ta-VNS) for patients with refractory epilepsy.Pre-trial?144 patients were randomly assigned to ta-VNS group (n=98) or transcutaneous auricular non-vagus nerve stimulation (tn-VNS) control group (n=46). Treatment was conducted twice per day for 24 weeks. After 8, 16 and 24 weeks of treatment?the patients were evaluated according to the Modified Engel Scale (four classes). After 8 weeks?according to the medical ethic design, patients in tn-VNS group were switched into ta-VNS group uniquely. After 8 weeks' treatment 41.0% and 27.5% of patients from ta-VNS and tn-VNS groups, respectively, experienced reduction in seizure frequency that reached I, II and III levels according to the standards of the Modified Engel Scale compared with the baselines, indicating significant difference in seizure reduction between the two groups. After 24 weeks of treatment, ta-VNS patients had a 47.7% reduction, and tn-VNS, with an additional 16 weeks of treatment, reached 47.5% in reduction. After 8 weeks' treatment, the percentages of average seizure frequency in ta-VNS and tn-VNS were reduced by 42.6% and 11.5% respectively, providing a statistically significant difference in the results between the two groups (P<0.05). In addition, there were significant improvements in electroencephalograph (EEG) and the quality of daily life of the patients after treatment.The results show that this ta-VNS treatment can effectively reduce the frequency of seizures and improve the patient's quality of life. This may be an effective treatment for refractory epilepsy. At the same time?it is also safe?economic, and widely applicable.
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Accelerating charging dynamics in subnanometre pores.
Nat Mater
PUBLISHED: 02-10-2014
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Supercapacitors have exceptional power density and cyclability but smaller energy density than batteries. Their energy density can be increased using ionic liquids and electrodes with subnanometre pores, but this tends to reduce their power density and compromise the key advantage of supercapacitors. To help address this issue through material optimization, here we unravel the mechanisms of charging subnanometre pores with ionic liquids using molecular dynamics simulations, navigated by a phenomenological model. We show that charging of ionophilic pores is a diffusive process, often accompanied by overfilling followed by de-filling. In sharp contrast to conventional expectations, charging is fast because ion diffusion during charging can be an order of magnitude faster than in the bulk, and charging itself is accelerated by the onset of collective modes. Further acceleration can be achieved using ionophobic pores by eliminating overfilling/de-filling and thus leading to charging behaviour qualitatively different from that in conventional, ionophilic pores.
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Temporal and spatial expression of the four Igf ligands and two Igf type 1 receptors in zebrafish during early embryonic development.
Gene Expr. Patterns
PUBLISHED: 02-07-2014
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The insulin-like growth factor (Igf) family is an evolutionarily conserved system essential for normal growth and development in vertebrates. Unlike mammals, four distinct Igf ligands (Igf1, Igf2a, Igf2b and Igf3) and two Igf type 1 receptors (Igf1ra and Igf1rb) are present in zebrafish. However, the localization of these multiple ligands and receptors especially the recently discovered igf3 during early development of zebrafish is poorly understood. In this study, detailed expression patterns of these components of the Igf system during embryogenesis of zebrafish were analyzed. It was found that igf1 is specifically expressed in the trigeminal ganglia region from 18 hpf to 72 hpf, while igf2a is restricted to the caudal regions of the notochord from 14 hpf to 18 hpf as well as in the midbrain, dorsal hind brain and otic vesicle at 24 hpf. On the other hand, igf2a is highly expressed in the midbrain and pharyngeal arch region at 48 hpf, followed by its appearance in the liver and brain at 72 hpf, while igf2b is restricted to the floor plate and hypochord from 12 hpf to 18 hpf, and strong expression is also detected in the midbrain and dorsal hind brain at 24 hpf. The teleost specific igf3 is highly expressed in the pharyngeal arch region before 24 hpf, but is then restricted to the sternohyoideus after 48 hpf. The receptor subtype igf1ra is ubiquitously expressed before 24 hpf but is confined to the brain at 72 hpf. However, igf1rb is widely expressed before 10 hpf, but is more confined to the brain region at 24 hpf and 72 hpf. This dynamic temporal-spatial expression during embryogenesis of zebrafish, together with the unique and overlapping expression patterns of the Igf ligands and receptors suggest the coordination of the divergent functions of the Igf system during early development in zebrafish.
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Comparison of patients hospitalized with influenza A subtypes H7N9, H5N1, and 2009 pandemic H1N1.
Clin. Infect. Dis.
PUBLISHED: 01-31-2014
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Influenza A(H7N9) viruses isolated from humans show features suggesting partial adaptation to mammals. To provide insights into the pathogenesis of H7N9 virus infection, we compared risk factors, clinical presentation, and progression of patients hospitalized with H7N9, H5N1, and 2009 pandemic H1N1 (pH1N1) virus infections.
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Nano conductive ceramic wedged graphene composites as highly efficient metal supports for oxygen reduction.
Sci Rep
PUBLISHED: 01-17-2014
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A novel conductive ceramic/graphene nanocomposite is prepared to prohibit the re-stacking of reduced graphene oxide (RGO) by wedging zirconium diboride (ZrB2) nanoparticles (NPs) into multiple layer nanosheets using a simple solvothermal method. Surprisingly, the RGO/ZrB2 nanocomposite supported Pt NPs shows very excellent catalytic activity. Its electrochemical surface area (ECSA) is up to 148?m(2)g(-1) (very approaches the geometry surface area of 155?m(2)g(-1)), much greater than that of the previous report (usually less than 100?m(2)g(-1)). The mass activity is as high as 16.8?A/g(-1), which is almost 2 times and 5 times that of Pt/RGO (8.6?A/g(-1)) and Pt/C (3.2?A/g(-1)), respectively, as benchmarks. Moreover, after 4000 cycles the catalyst shows only 61% of ECSA loss, meaning a predominantly electrochemical stability. The remarkably improved electrochemical properties with much high Pt utilization of the new catalyst show a promising application in low temperature fuel cells and broader fields.
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Combination of percutaneous unilateral translaminar facet screw fixation and interbody fusion for treatment of lower lumbar vertebra diseases: a follow-up study.
Orthop Surg
PUBLISHED: 01-14-2014
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To investigate the feasibility and efficacy of sight-guided percutaneous unilateral translaminar facet screw fixation (TLFSF) with interbody fusion for treatment of lower lumbar vertebra diseases.
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Accuracy of epidemiological inferences based on publicly available information: retrospective comparative analysis of line lists of human cases infected with influenza A(H7N9) in China.
BMC Med
PUBLISHED: 01-02-2014
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Appropriate public health responses to infectious disease threats should be based on best-available evidence, which requires timely reliable data for appropriate analysis. During the early stages of epidemics, analysis of 'line lists' with detailed information on laboratory-confirmed cases can provide important insights into the epidemiology of a specific disease. The objective of the present study was to investigate the extent to which reliable epidemiologic inferences could be made from publicly-available epidemiologic data of human infection with influenza A(H7N9) virus.
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Direct determination of mercury in cosmetic samples by isotope dilution inductively coupled plasma mass spectrometry after dissolution with formic acid.
Anal. Chim. Acta
PUBLISHED: 01-02-2014
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A new method was proposed for the accurate determination of mercury in cosmetic samples based on isotopic dilution (ID)-photochemical vapor generation (PVG)-inductively coupled plasma mass spectrometry (ICP MS) measurement. Cosmetic samples were directly dissolved in formic acid solution and subsequently subjected to PVG for the reduction of mercury into vapor species following by ICP MS detection. Therefore, the risks of analyte contamination and loss were avoided. Highly enriched (201)Hg isotopic spike is added to cosmetics and the isotope ratios of (201)Hg/(202)Hg were measured for the quantitation of mercury. With ID calibration, the influences originating from sample matrixes for the determination of mercury in cosmetic samples have been efficiently eliminated. The effects of several experimental parameters, such as the concentration of the formic acid, and the flow rates of carrier gas and sample were investigated. The method provided good reproducibility and the detection limits were found to be 0.6 pg mL(-1). Finally, the developed method was successfully applied for the determination of mercury in six cosmetic samples and a spike test was performed to verify the accuracy of the method.
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Risk Factors of Synchronous Inguinal Lymph Nodes Metastasis for Lower Rectal Cancer Involving the Anal Canal.
PLoS ONE
PUBLISHED: 01-01-2014
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The aim of the study is to identify the risk factors of synchronous ILN metastasis for lower rectal cancer involving the anal canal.
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Analysis of age and gender associated N-glycoproteome in human whole saliva.
Clin Proteomics
PUBLISHED: 01-01-2014
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Glycoproteins comprise a large portion of the salivary proteome and have great potential for biomarker discovery and disease diagnosis. However, the rate of production and the concentration of whole saliva change with age, gender and physiological states of the human body. Therefore, a thorough understanding of the salivary glycoproteome of healthy individuals of different ages and genders is a prerequisite for saliva to have clinical utility.
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Alpha-2-glycoprotein 1(AZGP1) regulates biological behaviors of LoVo cells by down-regulating mTOR signaling pathway and endogenous fatty acid synthesis.
PLoS ONE
PUBLISHED: 01-01-2014
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AZGP1 is a multifaceted protein associated with lipid mobilization, a process that is regulated by FASN and other metabolic pathways such as mTOR signaling. The active mTOR signaling pathway has been found to be involved in a variety of tumors. However, it remains unclear whether it is involved in the regulation of AZGP1 and FASN. An AZGP1-expressing plasmid was transfected into a human colorectal cancer cell line (LoVo) with a low expression of AZGP1. The expression of AZGP1, FASN, eIF4E, p-mTOR, p-S6,and S6K1 were measured by Western blot analysis, and target genes were detected by RT-PCR. Cell proliferation was studied using the MTT and colony formation assays. The analysis of apoptosis and the cell cycle phase were assessed by flow cytometry. The capacity of cell migration was investigated using the transwell migration assay. We found that the expression of AZGP1 was up-regulated while the expression of FASN, eIF4E, p-mTOR, p-S6, and S6K1 were down-regulated in LoVo cells after AZGP1 was expressed. The proliferation of malignant cells was reduced in AZGP1-overexpression cells, which is consistent with an increased in the G2-arrest and apoptosis rate. Furthermore, the migration of AZGP1-overexpression cells was decreased. The overexpression of AZGP1 suppressed the activation of the mTOR pathway and endogenous FASN-regulated fatty acid synthesis, mitigating the malignant phenotype of LoVo cells. Herein, we provide evidence that AZGP1 may constitute a novel tumor suppressor for LoVo colorectal cancer cells.
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Population-based hospitalization burden of influenza a virus subtypes and antigenic drift variants in children in Hong Kong (2004-2011).
PLoS ONE
PUBLISHED: 01-01-2014
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We aim to document and analyze influenza hospitalization burden in light of antigenic changes in circulating influenza viruses in Hong Kong.
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Rural villagers and urban residents exposure to poultry in China.
PLoS ONE
PUBLISHED: 01-01-2014
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Patterns of poultry exposure in rural and urban areas in China have not been systematically evaluated and compared. The objective of our study is to investigate patterns in human exposure to poultry in rural and urban China. We conducted a two-stage household-based clustered survey on population exposure to live/sick/dead poultry in Xiuning and Shenzhen. Half of the rural households (51%) in Xiuning raised poultry, mostly (78%) free-range. Around half of those households (40%) allowed poultry to stay in their living areas. One quarter of villagers reported having contact with sick or dead poultry. In Shenzhen, 37% urban residents visited live poultry markets. Among these, 40% purchased live poultry and 16% touched the poultry or cages during purchase. Our findings indicated that human exposure to poultry was different in rural and urban areas in China. This discrepancy could contribute to the observed differences in epidemiologic characteristics between urban and rural cases of influenza A(H7N9) and A(H5N1) virus infection.
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A systematic comparison of the anti-tumoural activity and toxicity of the three Adv-TKs.
PLoS ONE
PUBLISHED: 01-01-2014
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Adenovirus 5 vectors, known respectively as, the first generation, second generation and oncolytic adenovirus, have been studied extensively in preclinical and clinical trials. However, hitherto few systemic evaluations of the efficacy and toxicity of these adenoviral vectors that have reflected the vertical history of adenovirus based cancer gene therapy strategies have been undertaken. This study has chosen Adv-TK, the well-established adjuvant treatment in cancer, and compared its efficacy and safety with those of the two newly synthesized oncolytic adenovirus vectors encoding the HSV-TK gene, namely M7 and M8. The results obtained showed that systemic administration of 1×10(8) pfu M7 had an anti-tumour efficacy similar to that of 3×10(10) pfu Adv-TK whilst M8 performed even better. Furthermore, compared to Adv-TK, M7 and M8 reduced the incidence of metastases and substantially prolonged the survival time of the mice xenografted with human orthotopic gastric carcinomas with disseminated metastasis. Even more exciting, however, were the similar toxic and immune safety results obtained from the administration of high doses of M7 or M8 in comparison with Adv-TK in immunocompetent and permissive syrian hamster. The data here exhibit a comprehensive display of the efficacy and safety of the three mutants and provide evidence for the future preclinical use of the M7 and M8 viruses.
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Trichostatin A targets the mitochondrial respiratory chain, increasing mitochondrial reactive oxygen species production to trigger apoptosis in human breast cancer cells.
PLoS ONE
PUBLISHED: 01-01-2014
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Histone deacetylase inhibitors (HDACIs)-based therapies have stimulated interest via their anti-tumor activities, including apoptosis induction, cell cycle arrest, cell differentiation, and autophagy. However, the mechanisms of HDACI-associated anti-tumor activity are not yet clearly defined. The aim of this study was to explore the key events of Trichostatin A (TSA), a classic HDACI agent, against breast cancer cells.
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Angiotensin II (AngII) stimulates basolateral 50 pS K channels in the thick ascending limb.
Am. J. Physiol. Renal Physiol.
PUBLISHED: 12-26-2013
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We used the patch-clamp technique to examine the effect of angiotensin II (AngII) on the basolateral K channels in the thick ascending limb (TAL) of the rat kidney. Application of AngII increased the channel activity and the current amplitude of the basolateral 50 pS K channel. The stimulatory effect of AngII on the K channels was completely abolished by losartan, an inhibitor of type 1 angiotensin receptor (AT1R), but not by PD123319, an AT2R antagonist. Moreover, inhibition of phospholipase C (PLC) and protein kinase C (PKC) also abrogated the stimulatory effect of AngII on the basolateral K channels in the TAL. This suggests that the stimulatory effect of AngII on the K channels was induced by activating PLC and PKC pathways. Western blotting demonstrated that AngII increased the phosphorylation of c-Src in tyrosine residue 416, an indication of c-Src activation. This effect was mimicked by PKC stimulator but abolished by calphostin C. Moreover, inhibition of NADPH oxidase (NOX) also blocked the effect of AngII on c-Src tyrosine phosphorylation. The role of Src-family protein tyrosine kinase (SFK) in mediating the effect of AngII on the basolateral K channel was further suggested by the experiments in which inhibition of SFK abrogated the stimulatory effect of AngII on the basolateral 50 pS K channel. We conclude that AngII increases basolateral 50 pS K channels activity via AT1R and that activation of AT1R stimulates SFK by a PLC-PKC-NOX-dependent mechanism.
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Rat hippocampal responses up to 90 days after a single nanoceria dose extends a hierarchical oxidative stress model for nanoparticle toxicity.
Nanotoxicology
PUBLISHED: 12-18-2013
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Abstract Ceria engineered nanomaterials (ENMs) have very promising commercial and therapeutic applications. Few reports address the effects of nanoceria in intact mammals, let alone long term exposure. This knowledge is essential to understand potential therapeutic applications of nanoceria in relation to its hazard assessment. The current study elucidates oxidative stress responses in the rat hippocampus 1 and 20?h, and 1, 7, 30 and 90 days following a single systemic infusion of 30?nm nanoceria. The results are incorporated into a previously described hierarchical oxidative stress (HOS) model. During the 1-20?h period, increases of the GSSG: GSH ratio and cytoprotective phase-II antioxidants were observed. During the 1-7?d period, cytoprotective phase-II antioxidants activities were inhibited with concomitant elevation of protein carbonyl (PC), 3-nitrotyrosine (3NT), heme oxygenase-1 (HO-1), cytokine IL-1? and the autophagy marker LC-3AB. At 30 day post ceria infusion, oxidative stress had its major impact. Phase-II enzyme activities were inhibited; concurrently PC, 3NT, HO-1 and Hsp70 levels were elevated along with augmentation of IL-1?, pro-apoptotic pro-caspase-3 and LC-3AB levels. This progress of escalating oxidative stress was reversed at 90 days when phase-II enzyme levels and activities were restored to normal levels, PC and 3NT levels were reduced to baseline, cytokine and pro-caspase-3 levels were suppressed, and cellular redox balance was restored in the rat hippocampus. This study demonstrates that a single administration of nanoceria induced oxidative stress that escalates to 30 days then terminates, in spite of the previously reported continued presence of nanoceria in peripheral organs. These results for the first time confirm in vivo the HOS model of response to ENM previously posited based on in vitro studies and extends this prior hierarchical oxidative stress model that described three tiers to a 4th tier, characterized by resolution of the oxidative stress and return to normal conditions.
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Semicondutor quantum dots-based metal ion probes.
Nanoscale
PUBLISHED: 11-25-2013
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Semiconductor quantum dots (QDs) exhibit unique optical and photophysical properties that offer significant advantages over organic dyes as optical labels for chemo/bio-sensing. This review addresses the methods for metal ion detection with QDs, including photoluminescent, electrochemiluminescent, photoelectrochemical, and electrochemical approaches. The main mechanisms of direct interaction between QDs and metal ions which lead to photoluminescence being either off or on, are discussed in detail. These direct interactions provide great opportunities for developing simple yet effect metal ion probes. Different methods to design the chemically-modified QD hybrid structures through anchoring metal ion-specific groups onto the surface of QDs are summarized. Due to the spatial separation of the luminescence center and analyte recognition sites, these chemically-modified QDs offer greatly improved sensitivity and selectivity for metal ions. Several interesting applications of QD-based metal ion probes are presented, with specific emphasis on cellular probes, coding probes and sensing with logic gate operations.
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Effect of closure of live poultry markets on poultry-to-person transmission of avian influenza A H7N9 virus: an ecological study.
Lancet
PUBLISHED: 10-31-2013
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Transmission of the novel avian influenza A H7N9 virus seems to be predominantly between poultry and people. In the major Chinese cities of Shanghai, Hangzhou, Huzhou, and Nanjing-where most human cases of infection have occurred-live poultry markets (LPMs) were closed in April, 2013, soon after the initial outbreak, as a precautionary public health measure. Our objective was to quantify the effect of LPM closure in these cities on poultry-to-person transmission of avian influenza A H7N9 virus.
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miR-9 Regulation of BRCA1 and Ovarian Cancer Sensitivity to Cisplatin and PARP Inhibition.
J. Natl. Cancer Inst.
PUBLISHED: 10-29-2013
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Expression of BRCA1 is commonly decreased in sporadic ovarian cancer, and this is associated with platinum sensitivity and favorable prognosis. However, multiple mechanisms underlying low BRCA1 expression are not fully understood.
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Control of olfactory ensheathing cell behaviors by electrospun silk fibroin fibers.
Cell Transplant
PUBLISHED: 10-22-2013
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Olfactory ensheathing cells (OECs), the only glial cell type that normally penetrates the transition zone between the peripheral and central nervous systems, are one of the most promising candidates for cell transplantation in repairing spinal cord injury (SCI). However, we must manipulate and regulate OECs behavior to make these cells effective in cell transplantation. In the present study, we assessed the response of rat OECs to different variants of nanofibrous silk fibroin mats with regard to cell morphology, adhesion, proliferation, and migration and the related gene and protein expression. Results showed that OECs adhere and spread more easily on Tussah silk fibroin (TSF) fibers than Bombyx mori silk fibroin fibers, resulting in a higher rate of cell proliferation and gene and protein expression, examined by RT-PCR and ELISA. In addition, the morphology of OECs on microfibers is mainly polygonal with short protrusions, whereas the OECs on nanofibers exhibit a bipolar shape with long protrusions that align along the fibers, especially when aligned fibers are employed. Moreover, OECs on silk fibroin nanofibers migrate more efficiently than those on poly-L-lysine (PLL). Based on the experimental results, the morphology, adhesion, spread, gene and protein expression, and migration of OECs could be modulated and regulated by adjusting the contents and structure of silk fibroin nanofibers, shedding light on the control of OECs behavior in nerve tissue engineering and thus the future therapeutic intervention for nerve repair after injury. This manuscript is published as part of the International Association of Neurorestoratology (IANR) supplement issue of Cell Transplantation.
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Risk compensation behaviours in construction workers activities.
Int J Inj Contr Saf Promot
PUBLISHED: 10-19-2013
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The purpose of this study was to test whether the construction workers have the tendency of engaging in risk compensation behaviours, and identify the demographic variables, which may influence the extent to which the construction workers may show risk compensation behaviours. Both quantitative (survey) and qualitative (interviews) approaches were used in this study. A questionnaire survey was conducted with all the construction workers on three building construction sites of a leading construction company in Australia. Semi-structured interviews were then conducted to validate the findings of the quantitative research. The findings indicate that workers tend to show risk compensation behaviours in the construction environment. The workers with more working experience, higher education, or having never been injured at work before have a higher tendency to show risk compensation in their activities than the others. The implication is that contractors need to assess the potential influence of workers risk compensation behaviours when evaluating the effect of risk control measures. It is recommended that supervisors pay more attention to the behavioural changes of those workers who have more experience, higher education, and have never been injured before after the implementation of new safety control measures on construction site.
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Sensing during In Situ Growth of Mn-Doped ZnS QDs: A Phosphorescent Sensor for Detection of H2 S in Biological Samples.
Chemistry
PUBLISHED: 09-25-2013
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Monitoring the in situ growth of Mn-doped ZnS quantum dots is shown to be a route to selectively detect H2 S, an important endogenously produced signalling molecule. The use of Mn(2+) as a dopant resulted in orange phosphorescence, making it possible to avoid the background fluorescence from biological surroundings that can occur at other wavelengths. The choice of ZnS QDs as the host material ensured selectivity, since only sulfide can precipitate Zn(2+) and Mn(2+) from aqueous solution.
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[Influence of recombinant lentiviral vector encoding miR-15a/16-1 in biological features of human nasopharyngeal carcinoma CNE-2Z cells].
Zhonghua Er Bi Yan Hou Tou Jing Wai Ke Za Zhi
PUBLISHED: 09-11-2013
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To study the influence of recombinant lentiviral vector encoding miR-15a/16-1 on biological features of human nasopharyngeal carcinoma CNE-2Z cells and underlying mechanisms.
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Phosphorescent sensing of Cr3+ with protein-functionalized Mn-doped ZnS quantum dots.
Analyst
PUBLISHED: 09-04-2013
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It was found that the phosphorescence from denatured bovine serum albumin (dBSA)-capped Mn-doped ZnS QDs could be selectively quenched by Cr(3+), and a phosphorescent probe for Cr(3+) was thus developed. Based on phosphorescence decay as well as calculations of the relative energies of QDs and Cr(3+), the mechanism for phosphorescent quenching was preliminarily ascribed to electron transfer from photo-excited Mn-doped ZnS QDs to Cr(3+). Under the optimal conditions, good linear Stern-Volmer quenching was obtained for Cr(3+) in the range of 10 to 300 nM. The limit of detection of this phosphorescence probe (3 nM) was 1 to 2 orders of magnitude lower than those of previously reported nanosensors, owing to the effective elimination of background fluorescence and scattering from the sample matrix. The analytical potential of the proposed probe was evaluated through determination of Cr(3+) in water samples, with spike-recoveries ranging from 95 to 106%.
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Excess mortality impact of two epidemics of pandemic influenza A(H1N1pdm09) virus in Hong Kong.
Influenza Other Respir Viruses
PUBLISHED: 08-15-2013
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Hong Kong experienced two large epidemics of pandemic influenza A(H1N1pdm09). We used regression methods to estimate the excess mortality associated with each epidemic. The first epidemic of H1N1pdm09 peaked in September 2009 and was associated with 2·13 [95% confidence interval (CI): -8·08, 11·82] excess all-cause deaths per 100 000 population. The second epidemic of H1N1pdm09 in early 2011 was associated with 4·72 [95% CI: -0·70, 10·50] excess deaths per 100 000 population. More than half of the estimated excess all-cause deaths were attributable to respiratory causes in each epidemic. The reasons for substantial impact in the second wave remain to be clarified.
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Post-Synthesis Treatment gives Highly Stable Siliceous Zeolites through the Isomorphous Substitution of Silicon for Germanium in Germanosilicates.
Angew. Chem. Int. Ed. Engl.
PUBLISHED: 07-26-2013
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Germanosilicates, an important family of zeolites with increasing number of members and attractive porosities, but containing a large quantity of unstable Ge atoms in the framework, meet with great obstacles in terms of limited thermal and hydrothermal stability when it comes to practical use. A facile stabilization method thus has been developed to substitute isomorphously Ge atoms for Si atoms, giving rise to ultrastable siliceous analogues of the pristine germanosilicates.
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[Application of 3.0T susceptibility weighted imaging in the diagnosis of hemorrhagic foci and the outcome prediction of rabbits with brain blast injury].
Zhongguo Yi Xue Ke Xue Yuan Xue Bao
PUBLISHED: 07-06-2013
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To investigate the value of susceptibility weighted imaging(SWI)in the diagnosis of hemorrhagic foci early after blast injury and its role in the outcome prediction.
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Detection of mild to moderate influenza A/H7N9 infection by Chinas national sentinel surveillance system for influenza-like illness: case series.
BMJ
PUBLISHED: 06-27-2013
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To characterise the complete case series of influenza A/H7N9 infections as of 27 May 2013, detected by Chinas national sentinel surveillance system for influenza-like illness.
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Comparative epidemiology of human infections with avian influenza A H7N9 and H5N1 viruses in China: a population-based study of laboratory-confirmed cases.
Lancet
PUBLISHED: 06-24-2013
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The novel influenza A H7N9 virus emerged recently in mainland China, whereas the influenza A H5N1 virus has infected people in China since 2003. Both infections are thought to be mainly zoonotic. We aimed to compare the epidemiological characteristics of the complete series of laboratory-confirmed cases of both viruses in mainland China so far.
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Human infection with avian influenza A H7N9 virus: an assessment of clinical severity.
Lancet
PUBLISHED: 06-24-2013
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Characterisation of the severity profile of human infections with influenza viruses of animal origin is a part of pandemic risk assessment, and an important part of the assessment of disease epidemiology. Our objective was to assess the clinical severity of human infections with avian influenza A H7N9 virus, which emerged in China in early 2013.
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Single-stranded DNA as a cleavable linker for bioorthogonal click chemistry-based proteomics.
Bioconjug. Chem.
PUBLISHED: 05-17-2013
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In this communication, we report a new class of cleavable linker based on automatically synthesized, single-stranded DNAs. We incorporated a DNA oligo into an azide-functionalized biotin (biotin-DNA-N3) and used the probe to enrich for alkyne-tagged glycoproteins from mammalian cell lysates. Highly efficient and selective release of the captured proteins from streptavidin agarose resins was achieved using DNase treatment under very mild conditions. A total of 36 sialylated glycoproteins were identified from the lysates of HL60 cells, an acute human promyeloid leukemia cell line. These sialylated glycoproteins were involved in many different biological processes ranging from glycan biosynthesis to cell adhesion events.
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Inductively coupled plasma mass spectrometry-based immunoassay: A review.
Mass Spectrom Rev
PUBLISHED: 05-15-2013
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The last 10 years witnessed the emerging and growing up of inductively coupled plasma mass spectrometry (ICPMS)-based immunoassay. Its high sensitivity and multiplex potential have made ICPMS a revolutionary technique for bioanalyte quantification after element-tagged immunoassay. This review focuses on the major developments and the applications of ICPMS-based immunoassay, with emphasis on methodological innovations. The ICPMS-based immunoassay with elemental tags of metal ions, nanoparticles, and metal containing polymers was discussed in detail. The recent development of multiplex assay, mass cytometry, suspension array, and surface analysis demonstrated the versatility and great potential of this technique. ICPMS-based immunoassay has become one of the key methods in bioanalysis. © 2013 Wiley Periodicals, Inc. Mass Spec Rev, 2013.
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Solution-free, in situ preparation of nano/micro CuO/ZnO in dielectric barrier discharge for sensitive cataluminescence sensing of acetic acid.
Analyst
PUBLISHED: 05-15-2013
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The present work describes a new solution-free strategy for preparation of cluster-like nano/micro CuO/ZnO particles in dielectric barrier discharge (DBD) in which the brass acts as the inner electrode. The cataluminescence (CTL) behaviour of such prepared material for acetic acid was studied for analytical application. Under the optimized conditions, the linear range of CTL intensity versus concentration of acetic acid are 6 mg L(-1) to 500 mg L(-1) with the limit of detection (LOD) of 3 mg L(-1), no significant interference was found. The new method shows great advantages because it is a process without any solution and complex equipment. The synthetic material was directly used for the cataluminescence sensing of acetic acid without other preliminary treatment and it shows high selectivity, satisfactory stability, and better sensitivity and linearity.
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The prevalence and incidence of Parkinsons disease in China: a systematic review and meta-analysis.
J Neural Transm
PUBLISHED: 04-25-2013
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Parkinsons disease (PD) is a chronic neurodegenerative disorder affecting older individuals. There is inconsistent evidence about the prevalence and incidence of PD in China at present. The aim of the meta-analysis was to estimate the prevalence and incidence of PD and its relation to age, gender, and stage in China. The literature search was conducted using PubMed, EMBASE, Chinese Biological Medical Literature database (CBM), Chinese National Knowledge Infrastructure database (CNKI), Chinese Wanfang and Chongqing VIP database for studies investigating the prevalence and incidence of PD in China from the commencement of the database until August 2012; both English and Chinese publications were included. We estimated the prevalence and incidence of PD using meta-analysis. Thirteen eligible articles were collected. The results showed that the pooled prevalence and incidence of PD were 2 per 100,000 population and 797 per 100,000 person-years. A higher prevalence of PD was found in males than in females (OR 1.29, 95 % CI 1.05-1.57). The prevalence of PD increased with age: the highest was 1,663 per 100,000 in those aged 80 and older. The overall prevalence of PD is lower in China than in developed countries, but the incidence is higher than in some developed countries. Overall, the prevalence of PD appears to increase with age and there are sex differences evident in Chinese individuals.
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Synthesis and biological evaluation of novel benzyl-substituted (S)-phenylalanine derivatives as potent dipeptidyl peptidase 4 inhibitors.
Bioorg. Med. Chem.
PUBLISHED: 04-23-2013
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A series of novel benzyl-substituted (S)-phenylalanine derivatives were synthesized and evaluated for their dipeptidyl peptidase 4 (DPP-4) inhibitory activity and selectivity. It was found that most synthesized target compounds were potent DPP-4 inhibitors with IC50 values in 3.79-25.52 nM, which were significantly superior to that of the marketed drug sitagliptin. Furthermore, the 4-fluorobenzyl substituted phenylalanine derivative 6g not only displayed the potent DPP-4 inhibition with an IC50 value of 3.79 nM, but also showed better selectivity against DPP-4 over other related enzymes including DPP-7, DPP-8, and DPP-9. In an oral glucose tolerance test (OGTT) in normal Sprague Dawley rats, compound 6g reduced blood glucose excursion in a dose-dependent manner.
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Characterization and expression analysis of three CBF/DREB1 transcriptional factor genes from mangrove Avicennia marina.
Aquat. Toxicol.
PUBLISHED: 04-19-2013
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Three CBF/DREB1 (C-repeat binding factor/dehydration responsive element-binding factor 1) homologues were isolated from mangrove Avicennia marina and designated AmCBF1, 2 and 3. Multiple sequence analysis showed that the three deduced proteins all contain an AP2 DNA-binding domain and two CBF signature sequences. According to the phylogenetic analysis, these proteins belong to the A-1 subgroup of the DREB subfamily. Expression analyses based on quantitative real-time PCR revealed that the AmCBF2 displayed relatively high expression under normal conditions, with the highest level in stems, while both AmCBF1 and 3 were weakly expressed without stress. The three genes also showed different responses to various environmental stimuli. The AmCBF2 was inducible by cold, drought, high salinity, heavy metals, as well as abscisic acid (ABA), and exhibited much stronger induction by cold, drought, Pb(2+) or Zn(2+) than by NaCl, ABA or Cd(2+). In contrast, both AmCBF1 and AmCBF3 displayed insignificant changes under these stimuli. These results indicate that the three AmCBF genes play different roles in A. marina and the AmCBF2 might be involved in the signaling pathway of cold, drought and heavy metal stress response.
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[Effect of borate polymer layers on measurement of glucose concentration by SPR].
Guang Pu Xue Yu Guang Pu Fen Xi
PUBLISHED: 04-17-2013
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A new borate polymer PAA-ran-PAAPBA that can specifically adsorb glucose was introduced in the glucose measurement based on surface plasmon resonance, and the high-precision specific detection of glucose concentration was realized. Six layers and twelve layers of borate polymer were respectively bound onto the SPR sensors through the layer-by-layer self-assembly binding method, and the effect of different layers of borate polymer on the glucose surface plasmon resonance measurement was studied. The experiment was conducted in the concentration range of 1-10 mg x dL(-1) (interval delta = 1 mg x dL(-1)), 10-100 mg x dL(-1) (interval delta = 10 mg x dL(-1)), and 100-1 000 mg x dL(-1) (interval delta = 100 mg x dL(-1)), experiment data was fitted by quadric curve and the fitting degree of refractive index difference deltaRU and glucose concentration was obtained. Results showed that the 12-layer-polymer sensor was better than the 6-layer-polymer sensor in the first two smaller ranges, and the measuring result was not significantly affected by layers in the third range, indicating that for the small concentrations increasing polymer layer can dramatically improve the measurement.
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JoVE Visualize is a tool created to match the last 5 years of PubMed publications to methods in JoVE's video library.

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In developing our video relationships, we compare around 5 million PubMed articles to our library of over 4,500 methods videos. In some cases the language used in the PubMed abstracts makes matching that content to a JoVE video difficult. In other cases, there happens not to be any content in our video library that is relevant to the topic of a given abstract. In these cases, our algorithms are trying their best to display videos with relevant content, which can sometimes result in matched videos with only a slight relation.