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Find video protocols related to scientific articles indexed in Pubmed.
Sub-20 nm-Fe3O4 square and circular nanoplates: synthesis and facet-dependent magnetic and electrochemical properties.
Chem. Commun. (Camb.)
PUBLISHED: 11-10-2014
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We present an effective method to synthesize 15 nm magnetite nanocrystals with the morphology of square and circular nanoplates, which expose (001) facet and (111) facet, respectively. The magnetic property and electrochemical behavior towards As(iii) exhibit strong facet-dependent characteristics. Theoretical calculations confirm the facet-dependent characteristics and provide the corresponding explanations.
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[Autologous peripheral blood CD34+ stem cells transplanted into 100 patients with advanced cirrhosis].
Zhonghua Gan Zang Bing Za Zhi
PUBLISHED: 11-05-2014
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To investigate whether transplantation of autologous peripheral blood CD34+ stem cells is a viable approach for treating patients with advanced cirrhosis,which is currently hindered by a shortage in liver donors.
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p-Toluenesulfonic Acid Mediated 1,3-Dipolar Cycloaddition of Nitroolefins with NaN3 for Synthesis of 4-Aryl-NH-1,2,3-triazoles.
Org. Lett.
PUBLISHED: 10-24-2014
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A p-TsOH-mediated 1,3-dipolar cycloaddition of nitroolefins and sodium azide for the synthesis of 4-aryl-NH-1,2,3-triazoles has been developed. p-TsOH was discovered as a vital additive in this type of 1,3-dipolar cycloaddition. This novel cycloaddition reaction is a good method for the rapid synthesis of valuable 4-aryl-NH-1,2,3-triazoles in high yields.
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Uchl1 and its associated proteins were involved in spermatocyte apoptosis in mouse experimental cryptorchidism.
Sheng Li Xue Bao
PUBLISHED: 10-22-2014
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Uchl1 was found to be involved in spermatocyte apoptosis. The aim of the present study was to test whether Uchl1 and its associated proteins Jab1 and p27(kip1) were involved in spermatogenic damages in response to heat-stress in cryptorchidism. Hematoxylin and eosin (HE) staining and DNA end labeling (TUNEL) were used to observe morphological and apoptotic characteristics of spermatogenic cells; Immunohistochemical analysis was used to detect changes of Uchl1 and its associated proteins Jab1 and p27(kip1) in response to heat-stress from cryptorchidism leading to spermatocyte losses; And protein affinity analysis (pull-down) and immunofluorescence co-localization were used to verify the relevance among the three proteins in spermatocytes. The results showed that, Jab1 and p27(kip1), in parallel to Uchl1, increased in spermatocytes of apoptotic appearances in response to heat-stress, but not in multinucleated giant cells; Jab1 bound to Uchl1 in testis protein extracts, and co-localized with Uchl1 and p27(kip1) specifically in spermatocytes with apoptotic appearances. These results suggest that the accumulation of Uchl1 protein is involved in the heat-stress-induced spermatocyte apoptosis through a new pathway related with Jab1 and p27(kip1), but not the formation of multinucleated giant cells.
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Electrically tunable metasurface perfect absorbers for ultrathin mid-infrared optical modulators.
Nano Lett.
PUBLISHED: 10-20-2014
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Dynamically reconfigurable metasurfaces open up unprecedented opportunities in applications such as high capacity communications, dynamic beam shaping, hyperspectral imaging, and adaptive optics. The realization of high performance metasurface-based devices remains a great challenge due to very limited tuning ranges and modulation depths. Here we show that a widely tunable metasurface composed of optical antennas on graphene can be incorporated into a subwavelength-thick optical cavity to create an electrically tunable perfect absorber. By switching the absorber in and out of the critical coupling condition via the gate voltage applied on graphene, a modulation depth of up to 100% can be achieved. In particular, we demonstrated ultrathin (thickness < ?0/10) high speed (up to 20 GHz) optical modulators over a broad wavelength range (5-7 ?m). The operating wavelength can be scaled from the near-infrared to the terahertz by simply tailoring the metasurface and cavity dimensions.
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[Comparison of temporomandibular joint changes in adolescent Class II devision 1 malocclusion patients with mandibular retrusion treated with Twin-block and Class II elastics].
Zhonghua Kou Qiang Yi Xue Za Zhi
PUBLISHED: 09-27-2014
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To compare the temporomandibular joint changes in adolescent Class II division 1 malocclusion patients with mandibular retrusion treated with Twin- block and Class II elastics.
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[Ash2, a subunit of histone H3K4 methyltransferase complex, is involved in the sporulation in Schizosaccharomyces pombe].
Yi Chuan
PUBLISHED: 09-25-2014
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Schizosaccharomyces pombe undergoes meiosis instead of mitosis under conditions of nitrogen starvation and pheromone signalling, which results in conjugation and sporulation. During this progress, the pheromone-responsive MAPK(Mitogen-activated protein kinases) pathway plays an important role in regulating the conjuation and the transcriptional activation of genes required for meiosis. Spk1, a key component of MAPK pathway, activates Ste11 through protein phosphorylation and then induced the transcriptions of several genes requied for meiosis, including mei2(+), mam2(+) and map3(+). Methylation of histone H3K4 is involved in several important biological processes, including transcriptional activation and chromatin remodeling. However, its role in the sporualtion of fission yeast is poorly understood. Ash2 is a subunit of COMPASS, a conserved H3K4 methyltransferase complex. Sequence alignment analysis revealed that Ash2 in pombe shares two conserved domain with other homologues. Ash2 is localized in nucleus and contributes to methylation of H3K4. Deletion of ash2(+) resulted in a delay of sporulation and a substantial drop of sporulation efficiency. ChIP and qPCR analysis showed that deletion of ash2(+) caused a reduction of H3K4me2 level in the coding region of spk1(+), as well as a reduction of its mRNA level. Although the mRNA level of ste11(+) kept unchanged, the levels of Ste11-targetted genes, such as mei2(+), mam2(+) and map3(+), all reduced in ash2? cells. The results suggest that Ash2 regulates MAPK pathway and sporulation through H3K4 methylation. This might provide a new clue to elucidate the link between meiosis and epigenetic regulation.
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Palladium-catalyzed aminocarbonylation of aryl iodides with amides and N-alkyl anilines.
Chem Asian J
PUBLISHED: 09-10-2014
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A novel and efficient palladium-catalyzed aminocarbonylation of aryl iodides with amides and N-alkyl anilines has been developed. The reaction tolerates a wide range of functional groups and is a reliable method for the rapid synthesis of a variety of valuable imides and tertiary benzanilides under an atmospheric pressure of CO.
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Positional isomeric tunable two Co(II) 6-connected 3-D frameworks with pentanuclear to binuclear units: structures, ion-exchange and magnetic properties.
Dalton Trans
PUBLISHED: 09-06-2014
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Two new Co(II) based metal-organic frameworks, namely {[Co5(?3-OH)2(m-pda)3(bix)4]·2ClO4}n (1) and {[Co2(p-pda)2(bix)2(H2O)]·H2O}n (2), were prepared by hydrothermal reactions of Co(II) salt with two isomeric dicarboxyl tectons 1,3-phenylenediacetic acid (m-pda) and 1,4-phenylenediacetic acid (p-pda), along with 1,3-bis(imidazol-L-ylmethyl)benzene (bix). Both complexes 1 and 2 have been characterized by elemental analysis, IR spectroscopy, single-crystal X-ray diffraction, powder X-ray diffraction (PXRD), and thermogravimetric analysis (TGA). 1 shows a 6-connected 3-D pcu cationic framework with pentanuclear [Co5(?3-OH)2(COO)6(bix)2](2+) units, while 2 exhibits a 6-connected 3-D msw net based on [Co2(?2-H2O)(COO)2](2+) clusters. The results indicate that the different dispositions of the carboxylic groups of dicarboxylates have an important effect on the overall coordination frameworks. Perchlorate anions in 1 can be partly exchanged by thiocyanate and azide anions, however they are unavailable to nitrate anions. Magnetic susceptibility measurements indicate that both 1 and 2 show weak antiferromagnetic interactions between the adjacent Co(II) ions.
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Upregulation of chemokine receptor CCR10 is essential for glioma proliferation, invasion and patient survival.
Oncotarget
PUBLISHED: 08-24-2014
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Human gliomas are characterized by their invasion of normal brain structures irrespective of their grade of malignancy. Tumor cell invasion share many similarities with leukocyte trafficking, which is critically regulated by chemokines and their receptors. Here we report that the chemokine receptor CCR10 is highly expressed in human glioblastoma compared with control brain tissue. In vitro, signaling through CCL27-CCR10 mediates activation of p-Akt, and subsequently induces proliferation and invasive responses. Cell proliferation and invasion promoted by CCL27 were blocked by inhibition of p-Akt or CCR10. In vivo, down-regulation of CCR10 significantly impairs growth of glioma. Clinically, High CCR10 expression in GBM correlated with p-Akt, shorter overall survival and progression-free survival (P < 0.05). Together, these findings suggest that elevated CCR10 is a critical molecular event associated with gliomagenesis.
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SDF-1/CXCR4 promotes epithelial-mesenchymal transition and progression of colorectal cancer by activation of the Wnt/?-catenin signaling pathway.
Cancer Lett.
PUBLISHED: 08-20-2014
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Stromal cell-derived factor 1 (SDF-1) and its receptor, CXCR4, play an important role in angiogenesis and are associated with tumor progression. This study aimed to investigate the role of SDF-1/CXCR4-mediated epithelial-mesenchymal transition (EMT) and the progression of colorectal cancer (CRC) as well as the underlying mechanisms. The data showed that expression of CXCR4 and ?-catenin mRNA and protein was significantly higher in CRC tissues than in distant normal tissues. CXCR4 expression was associated with ?-catenin expression in CRC tissues, whereas high CXCR4 expression was strongly associated with low E-cadherin, high N-cadherin, and high vimentin expression, suggesting a cross talk between the SDF-1/CXCR4 axis and Wnt/?-catenin signaling pathway in CRC. In vitro, SDF-1 induced CXCR4-positive colorectal cancer cell invasion and EMT by activation of the Wnt/?-catenin signaling pathway. In contrast, SDF-1/CXCR4 axis activation-induced colorectal cancer invasion and EMT was effectively inhibited by the Wnt signaling pathway inhibitor Dickkopf-1. In conclusion, CXCR4-promoted CRC progression and EMT were regulated by the Wnt/?-catenin signaling pathway. Thus, targeting of the SDF-1/CXCR4 axis could have clinical applications in suppressing CRC progression.
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Bowl-like SnO2 @Carbon Hollow Particles as an Advanced Anode Material for Lithium-Ion Batteries.
Angew. Chem. Int. Ed. Engl.
PUBLISHED: 08-03-2014
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Despite the great advantages of hollow structures as electrodes for lithium-ion batteries, one apparent common drawback which is often criticized is their compromised volumetric energy density due to the introduced hollow interior. Here, we design and synthesize bowl-like SnO2 @carbon hollow particles to reduce the excessive hollow interior space while retaining the general advantages of hollow structures. As a result, the tap density can be increased about 30?%. The as-prepared bowl-like SnO2 @carbon hollow particles with conformal carbon support exhibit excellent lithium storage properties in terms of high capacity, stable cyclability and excellent rate capability.
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A Nonparametric Test of Missing Completely at Random for Incomplete Multivariate Data.
Psychometrika
PUBLISHED: 08-01-2014
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Missing data occur in many real world studies. Knowing the type of missing mechanisms is important for adopting appropriate statistical analysis procedure. Many statistical methods assume missing completely at random (MCAR) due to its simplicity. Therefore, it is necessary to test whether this assumption is satisfied before applying those procedures. In the literature, most of the procedures for testing MCAR were developed under normality assumption which is sometimes difficult to justify in practice. In this paper, we propose a nonparametric test of MCAR for incomplete multivariate data which does not require distributional assumptions. The proposed test is carried out by comparing the distributions of the observed data across different missing-pattern groups. We prove that the proposed test is consistent against any distributional differences in the observed data. Simulation shows that the proposed procedure has the Type I error well controlled at the nominal level for testing MCAR and also has good power against a variety of non-MCAR alternatives.
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Large scale screening of genetic interaction with sgf73(+) in fission yeast.
Yi Chuan
PUBLISHED: 07-31-2014
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Genetic interaction (GI) not only suggests functional correlations between different genes in vivo, but also provides clues for understanding the potential biological function of a specific gene. Screening of GI is an important method for understanding GI between different genes. In this study, we selected sgf73? as a query, which encodes a subunit of SAGA (Spt-Ada-Gcn5 acetyltransferase) complex deubiquitination module, to perform a large scale screening of GI in fission yeast. Our data showed that 164 genes had negative GIs whereas 42 genes had positive GIs with sgf73?. GO (Gene ontology) analysis indicated that these genes were enriched in several important biological processes, including chromatin modification, DNA damage repair, cellular response to stress, RNA transcription and so on. By using histone modification detection, we showed for the first time that loss of sgf73? led to a decreased level of histone acetylation at H3K9 and H4K16 and an increased level of histone H3K4 methylation. Furthermore, the spot assay results showed that the sgf73? cells exhibited increased sensitivity to DNA damage agents, HU and CPT, and sgf73? was involved in responses to hyperoxia stress. All these results suggested that sgf73? plays important roles in chromatin modification, DNA damage repair and hyperoxia responses.
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Performance analysis of single-frequency MPPSK integrated system for ranging applications.
ScientificWorldJournal
PUBLISHED: 07-21-2014
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The dual-frequency MPPSK-MODEM is a flexible platform. When ranging accuracy request is low or platform is particularly limited by power, the platform would perform both data transmission and range measurement with single-frequency modes. In this paper, the ranging resolution of MPPSK pulse waveforms with the match filter and impacting filter processing are discussed, respectively. Also, the parameter selection of MPPSK modulator for ranging is considered. In particular, requirements that allow for employing such special parameter values for range measurements with high accuracy and high range are investigated. Moreover, high repetition frequency (HRF) biphase code MPPSK pulse train base on m sequence is presented, and the ranging accuracy of the proposed signal with the match filter processing is deduced. In addition to theoretical considerations, the paper presents system simulations and measurement results of single-frequency MPPSK integrated systems, demonstrating the high-performance of ranging applications.
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Modeling the propagation of mobile phone virus under complex network.
ScientificWorldJournal
PUBLISHED: 07-15-2014
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Mobile phone virus is a rogue program written to propagate from one phone to another, which can take control of a mobile device by exploiting its vulnerabilities. In this paper the propagation model of mobile phone virus is tackled to understand how particular factors can affect its propagation and design effective containment strategies to suppress mobile phone virus. Two different propagation models of mobile phone viruses under the complex network are proposed in this paper. One is intended to describe the propagation of user-tricking virus, and the other is to describe the propagation of the vulnerability-exploiting virus. Based on the traditional epidemic models, the characteristics of mobile phone viruses and the network topology structure are incorporated into our models. A detailed analysis is conducted to analyze the propagation models. Through analysis, the stable infection-free equilibrium point and the stability condition are derived. Finally, considering the network topology, the numerical and simulation experiments are carried out. Results indicate that both models are correct and suitable for describing the spread of two different mobile phone viruses, respectively.
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Septin ring assembly is regulated by Spt20, a structural subunit of the SAGA complex.
J. Cell. Sci.
PUBLISHED: 07-11-2014
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Accurate cell division requires the proper assembly of high-order septin structures. In fission yeast (Schizosaccharomyces pombe), Spn1-Spn4 are assembled into a primary septin ring at the division site, and the subsequent recruitment of Mid2 to the structure results in a stable septin ring. However, not much is known about the regulation of this key process. Here, we found that deletion of Spt20, a structural subunit of the Spt-Ada-Gcn5-acetyltransferase (SAGA) transcriptional activation complex, caused a severe cell separation defect. The defect was mainly due to impaired septin ring assembly, as 80% of spt20? cells lost septin rings at the division sites. Spt20 regulates septin ring assembly partially through the transcriptional activation of mid2(+). Spt20 also interacted with Spn2 and Mid2 in vitro and was associated with other components of the ring in vivo. Spt20 colocalized with the septin ring, but did not separate when the septin ring split. Importantly, Spt20 regulated the stability of the septin ring and was required for the recruitment of Mid2. The transcription-dependent and -independent roles of Spt20 in septin ring assembly highlight a multifaceted regulation of one process by a SAGA subunit.
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Glioma-associated antigen HEATR1 induces functional cytotoxic T lymphocytes in patients with glioma.
J Immunol Res
PUBLISHED: 07-09-2014
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A2B5+ glioblastoma (GBM) cells have glioma stem-like cell (GSC) properties that are crucial to chemotherapy resistance and GBM relapse. T-cell-based antigens derived from A2B5+ GBM cells provide important information for immunotherapy. Here, we show that HEAT repeat containing 1 (HEATR1) expression in GBM tissues was significantly higher than that in control brain tissues. Furthermore, HEATR1 expression in A2B5+ U87 cells was higher than that in A2B5-U87 cells (P = 0.016). Six peptides of HEATR1 presented by HLA-A?02 were selected for testing of their ability to induce T-cell responses in patients with GBM. When peripheral blood mononuclear cells from healthy donors (n = 6) and patients with glioma (n = 33) were stimulated with the peptide mixture, eight patients with malignant gliomas had positive reactivity with a significantly increased number of responding T-cells. The peptides HEATR(1682-690), HEATR(11126-1134), and HEATR(1757-765) had high affinity for binding to HLA-A?02:01 and a strong capacity to induce CTL response. CTLs against HEATR1 peptides were capable of recognizing and lysing GBM cells and GSCs. These data are the first to demonstrate that HEATR1 could induce specific CTL responses targeting both GBM cells and GSCs, implicating that HEATR1 peptide-based immunotherapy could be a novel promising strategy for treating patients with GBM.
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Four uncommon nanocage-based Ln-MOFs: highly selective luminescent sensing for Cu²? ions and selective CO? capture.
Chem. Commun. (Camb.)
PUBLISHED: 06-25-2014
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Four uncommon isostructural nanocage-based 3D Ln-MOFs, 1-Ln (Ln = Eu, Tb, Gd and Dy), were constructed using a new diisophthalate ligand with active pyridyl sites. 1-Eu exhibits highly efficient luminescent sensing for Cu(2+) ions and selective CO2 capture.
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High-responsivity mid-infrared graphene detectors with antenna-enhanced photocarrier generation and collection.
Nano Lett.
PUBLISHED: 06-20-2014
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Graphene is an attractive photoconductive material for optical detection due to its broad absorption spectrum and ultrashort response time. However, it remains a great challenge to achieve high responsivity in graphene detectors because of graphene's weak optical absorption (only 2.3% in the monolayer graphene sheet) and short photocarrier lifetime (<1 ps). Here we show that metallic antenna structures can be designed to simultaneously improve both light absorption and photocarrier collection in graphene detectors. The coupled antennas concentrate free space light into the nanoscale deep-subwavelength antenna gaps, where the graphene light interaction is greatly enhanced as a result of the ultrahigh electric field intensity inside the gap. Meanwhile, the metallic antennas are designed to serve as electrodes that collect the generated photocarriers very efficiently. We also elucidate the mechanism of photoconductive gain in the graphene detectors and demonstrate mid-infrared (mid-IR) antenna-assisted graphene detectors at room temperature with more than 200 times enhancement of responsivity (?0.4 V/W at ?0 = 4.45 ?m) compared to devices without antennas (<2 mV/W).
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B7-H3 and B7-H1 expression in cerebral spinal fluid and tumor tissue correlates with the malignancy grade of glioma patients.
Oncol Lett
PUBLISHED: 06-19-2014
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The B7 family consists of activating and inhibitory molecules that regulate immune responses. Recent research demonstrated the roles of soluble B7-H3 (sB7-H3) and soluble B7-H1 (sB7-H1) in the blood serum of various tumors; however, none of these studies investigated the expression of these proteins in the cerebral spinal fluid (CSF) and blood serum of patients with glioma. The aim of the present study was to investigate the expression of B7-H3 and B7-H1 in the CSF, blood serum and tissues of patients with glioma and their correlation with clinicopathological data. Between January 2012 and November 2012, samples were obtained from 78 patients with glioma, four CSF samples were obtained from patients with a moderate traumatic brain injury, four brain tissue samples were obtained from patients with a traumatic brain injury and 40 blood serum samples were obtained from healthy individuals. The expression of B7-H3 and B7-H1 in the CSF, blood serum and tumor samples of the patients with high-grade glioma was found to be higher than that in the patients with low-grade glioma. However, no significant differences in sB7-H3 and sB7-H1 expression were observed in the blood serum of the patients with glioma compared with the healthy control subjects. In addition, the expression of sB7-H3 and sB7-H1 in the CSF of the patients with glioma was higher than that in the CSF of the patients with a moderate traumatic brain injury. Furthermore, in the patients with glioma, B7-H3 and B7-H1 expression in the CSF and tumor tissue, although not in the blood serum, correlated with the glioma grade.
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Flexible Organic Electronics in Biology: Materials and Devices.
Adv. Mater. Weinheim
PUBLISHED: 06-13-2014
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At the convergence of organic electronics and biology, organic bioelectronics attracts great scientific interest. The potential applications of organic semiconductors to reversibly transmit biological signals or stimulate biological tissues inspires many research groups to explore the use of organic electronics in biological systems. Considering the surfaces of movable living tissues being arbitrarily curved at physiological environments, the flexibility of organic bioelectronic devices is of paramount importance in enabling stable and reliable performances by improving the contact and interaction of the devices with biological systems. Significant advances in flexible organic bio-electronics have been achieved in the areas of flexible organic thin film transistors (OTFTs), polymer electrodes, smart textiles, organic electrochemical ion pumps (OEIPs), ion bipolar junction transistors (IBJTs) and chemiresistors. This review will firstly discuss the materials used in flexible organic bioelectronics, which is followed by an overview on various types of flexible organic bioelectronic devices. The versatility of flexible organic bioelectronics promises a bright future for this emerging area.
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Palladium-catalyzed oxidative cyclization of tertiary enamines for synthesis of 1,3,4-trisubstituted pyrroles and 1,3-disubstituted indoles.
Org. Lett.
PUBLISHED: 06-12-2014
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A novel and efficient palladium-catalyzed intramolecular oxidative cyclization of tertiary enamines for the synthesis of 1,3,4-trisubstituted pyrroles and 1,3-disubstituted indoles has been developed. Trifluoroacetic acid plays an important role in the reaction. A series of pyrroles and indoles with substitution patterns that are not easily accessible by traditional routes were synthesized in good yields under mild conditions.
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Comparison of diffusive gradients in thin film technique with traditional methods for evaluation of zinc bioavailability in soils.
Environ Monit Assess
PUBLISHED: 06-11-2014
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The technique of diffusive gradients in thin film (DGT) has been shown to be a promising tool to assess zinc (Zn) bioavailability in soils, but there exists considerable debate on its suitability. In this study, Zn bioavailability was systematically investigated in wheat- and maize-grown soils using this technique and seven traditional methods, including soil solution concentration and six widely used single-step extraction methods (HAc, EDTA, NaAc, NH4Ac, CaCl?, and MgCl?). The concentrations of Zn in the shoots and roots of these two plant species increased continuously with increasing additions of Zn to the soils, accompanied by significant decreases in shoot biomass and root biomass at Zn concentrations greater than 400 mg kg(-1) for maize and 800 mg kg(-1) for wheat. Zinc uptake and accumulation was higher in maize roots than in wheat roots. Both the concentrations of bioavailable Zn measured by DGT (CDGT) and soil solutions (Csol) increased linearly with increasing additions of Zn to the soils, while no strong linear relationships were observed for the extraction methods. Higher concentrations of extractable Zn, lower values of Csol, and larger values of R (i.e., the ratio of CDGT to Csol) were observed in maize-grown soils compared with those of wheat-grown soils, while the values of C DGT between the two plants were similar. These findings demonstrate that there likely exists a stronger resupply of Zn from the soil solid phases in maize-grown soils to satisfy a higher Zn uptake and accumulation in this plant. Linear correlation analyses showed that CDGT had the highest correlation coefficients with plant Zn concentrations compared with other traditional methods, implying that the DGT technique is more sensitive and robust in reflecting Zn bioavailability in soils to plants.
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Metal ions and solvents ratio co-regulate four new magnetic coordination polymers based upon an unsymmetric tricarboxylate acid ligand.
Dalton Trans
PUBLISHED: 06-07-2014
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Four new coordination polymers (CPs), [Co3(L)2(bib)3(EtOH)2]·2H2O (1), [Mn3(L)2(bib)2(H2O)4]·4H2O (2), [Ni(HL)(bib)(H2O)3] (3) and [Ni3(bib)4(H2O)10]·2(L)·12(H2O) (4), were synthesized under solvothermal conditions with an unsymmetrical tricarboxylic acid ligand [biphenyl-3,3',5-tricarboxylic acid (H3L)] in the presence of the 1,4-bis(1H-imidazol-4-yl)benzene (bib) ligand. In compound 1, tricarboxylate acid ligands (H3L) link Co centers to generate two-dimensional (2D) layers which are further connected by bib ligands to exhibit a three-dimensional (3D) framework with a (4,4,5)-connected (4(2)·6(3)·8)2(4(2)·6(7)·8)2(6(4)·8·10) topology. The three-dimensional (3D) framework of 2 is defined as a (3,3,4)-connected topology with the point symbol of (6·8(2))2(6(2)·8)2(8·10(4)·12). Compound 3 contains a one-dimensional (1D) left-hand helix chain along the a direction and further extends into a 2D supramolecular network and a 3D supramolecular framework via hydrogen bonds. Compound 4 displays a one-dimensional (1D) molecular ladder, which is further combined with each other through ?···? stacking to extend into 2D supramolecular sheets. The supramolecular networks of 3 and 4 resulted from the different solvent ratios [V(H2O)-V(EtOH)] in the reaction. All the CPs are characterized by single-crystal X-ray diffraction analyses, powder X-ray diffraction (PXRD), elemental analyses, IR spectroscopy, and TGA analyses. Moreover, the weak ferromagnetic properties of 2 and 3 and antiferromagnetic properties of 1 and 4 have also been investigated.
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A facile BPO-mediated ortho-hydroxylation and benzoylation of N-alkyl anilines for synthesis of 2-benzamidophenols.
Org. Lett.
PUBLISHED: 06-04-2014
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A facile benzoyl peroxide (BPO) mediated ortho-hydroxylation and benzoylation of N-alkyl anilines for the synthesis of 2-benzamidophenols has been developed. The reaction tolerates a wide range of functional groups and is a good method for the straightforward synthesis of valuable 2-benzamidophenols in good yields under mild conditions.
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Copper-catalyzed 5-endo-trig cyclization of ketoxime carboxylates: a facile synthesis of 2-arylpyrroles.
Chem. Commun. (Camb.)
PUBLISHED: 05-31-2014
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A novel and facile copper-catalyzed 5-endo-trig cyclization of ketoxime carboxylates for the synthesis of 2-arylpyrroles has been developed. The reaction tolerates a range of functional groups and is a practical procedure for rapid synthesis of 2-arylpyrroles in high yields under mild conditions.
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In vitro synergistic antitumor efficacy of sequentially combined chemotherapy/icotinib in non?small cell lung cancer cell lines.
Oncol. Rep.
PUBLISHED: 05-28-2014
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The concurrent administration of chemotherapy and epidermal growth factor receptor?tyrosine kinase inhibitors (EGFR?TKIs) has previously produced a negative interaction and failed to confer a survival benefit to non?small cell lung cancer (NSCLC) patients compared with first?line cytotoxic chemotherapy. The present study aimed to investigate the optimal schedule of the combined treatment of cisplatin/paclitaxel and icotinib in NSCLC cell lines and clarify the underlying mechanisms. HCC827, H1975, H1299 and A549 human NSCLC cell lines with wild?type and mutant EGFR genes were used as in vitro models to define the differential effects of various schedules of cisplatin/paclitaxel with icotinib treatments on cell growth, proliferation, cell cycle distribution, apoptosis, and EGFR signaling pathway. Sequence?dependent antiproliferative effects differed among the four NSCLC cell lines, and were not associated with EGFR mutation, constitutive expression levels of EGFR or downstream signaling molecules. The antiproliferative effect of cisplatin plus paclitaxel followed by icotinib was superior to that of cisplatin or paclitaxel followed by icotinib in the HCC827, H1975, H1299 and A549 cell lines, and induced more cell apoptosis and G0/G1 phase arrest. Cisplatin and paclitaxel significantly increased the expression of EGFR phosphorylation in the HCC827 cell line. However, only paclitaxel increased the expression of EGFR phosphorylation in the H1975 cell line. Cisplatin/paclitaxel followed by icotinib influenced the expression of p?EGFR and p?AKT, although the expression of p?ERK1/2 remained unchanged. The results suggest that the optimal schedule of the combined treatment of cisplatin/paclitaxel and icotinib differed among the NSCLC cell lines. The results also provide molecular evidence to support clinical treatment strategies for NSCLC patients.
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Ruthenium-catalyzed cyclization of ketoxime acetates with DMF for synthesis of symmetrical pyridines.
Org. Lett.
PUBLISHED: 05-13-2014
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A novel ruthenium-catalyzed cyclization of ketoxime carboxylates with N,N-dimethylformamide (DMF) for the synthesis of tetrasubstituted symmetrical pyridines has been developed. A methyl carbon on DMF performed as a source of a one carbon synthon. And NaHSO3 plays a role in the reaction.
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Effect of Danshao Huaxian capsule on Gremlin and bone morphogenetic protein-7 expression in hepatic fibrosis in rats.
World J. Gastroenterol.
PUBLISHED: 04-30-2014
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To observe the effect of Danshao Huaxian capsule (DHC) on the expression of Gremlin and bone morphogenetic protein-7 (BMP-7) in the liver of hepatic fibrosis rats.
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Trim58 degrades Dynein and regulates terminal erythropoiesis.
Dev. Cell
PUBLISHED: 04-24-2014
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TRIM58 is an E3 ubiquitin ligase superfamily member implicated by genome-wide association studies to regulate human erythrocyte traits. Here, we show that Trim58 expression is induced during late erythropoiesis and that its depletion by small hairpin RNAs (shRNAs) inhibits the maturation of late-stage nucleated erythroblasts to anucleate reticulocytes. Imaging flow cytometry studies demonstrate that Trim58 regulates polarization and/or extrusion of erythroblast nuclei. In vitro, Trim58 directly binds and ubiquitinates the intermediate chain of the microtubule motor dynein. In cells, Trim58 stimulates proteasome-dependent degradation of the dynein holoprotein complex. During erythropoiesis, Trim58 expression, dynein loss, and enucleation occur concomitantly, and all are inhibited by Trim58 shRNAs. Dynein regulates nuclear positioning and microtubule organization, both of which undergo dramatic changes during erythroblast enucleation. Thus, we propose that Trim58 promotes this process by eliminating dynein. Our findings identify an erythroid-specific regulator of enucleation and elucidate a previously unrecognized mechanism for controlling dynein activity.
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Palladium-catalyzed carbonylation of o-iodoanilines for synthesis of isatoic anhydrides.
J. Org. Chem.
PUBLISHED: 04-18-2014
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A novel palladium-catalyzed oxidative double carbonylation of o-iodoanilines for the synthesis of isatoic anhydrides has been developed. The reaction employs readily available o-iodoanilines as the starting materials and proceeds under mild conditions. For extension, palladium-catalyzed oxidative carbonylation of anthranilic acids was developed for the synthesis of substituted isatoic anhydrides in high to excellent yields.
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Clinical outcome of autologous hematopoietic stem cell infusion via hepatic artery or portal vein in patients with end-stage liver diseases.
Chin. Med. Sci. J.
PUBLISHED: 04-05-2014
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To investigate the efficacy of hematopoietic stem cell (HSC) transplantation via the hepatic artery vs. the portal vein for end-stage liver disease (ESLD).
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Reference values for healthy human myocardium using a T1 mapping methodology: results from the International T1 Multicenter cardiovascular magnetic resonance study.
J Cardiovasc Magn Reson
PUBLISHED: 03-26-2014
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T1 mapping is a robust and highly reproducible application to quantify myocardial relaxation of longitudinal magnetisation. Available T1 mapping methods are presently site and vendor specific, with variable accuracy and precision of T1 values between the systems and sequences. We assessed the transferability of a T1 mapping method and determined the reference values of healthy human myocardium in a multicenter setting.
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Level IB nodal involvement in oropharyngeal carcinoma: Implications for submandibular gland-sparing intensity-modulated radiotherapy.
Laryngoscope
PUBLISHED: 03-17-2014
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Submandibular gland-sparing intensity-modulated radiotherapy (SMG-sparing IMRT) has been proposed to reduce xerostomia following head and neck irradiation. However, the safety of this practice has been questioned. Data from a large surgical series of oropharyngeal carcinoma patients were extracted to identify clinicopathological correlates for submandibular involvement and to create a risk stratification scheme to guide decision making to refine selection guidelines for SMG-sparing IMRT.
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Input-output finite-time stabilization of linear systems with finite-time boundedness.
ISA Trans
PUBLISHED: 03-16-2014
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The paper presents linear system Input-Output Finite-Time Stabilization (IO-FTS) method under Finite-Time Boundedness (FTB) constraint. A state feedback controller is designed, via Linear Matrix Inequalities (LMIs), to guarantee the system both IO-FTS and FTB. The proposed methods are applied to the guidance design of a class of terminal guidance systems to suppress disturbances with IO-FTS method and FTB constraints simultaneously satisfied. The simulation results illustrate the effectiveness of the proposed methods.
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Anti-apoptotic and apoptotic pathway analysis of arsenic trioxide?induced apoptosis in human gastric cancer SGC-7901 cells.
Oncol. Rep.
PUBLISHED: 03-13-2014
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The present study aimed to investigate the effect of arsenic trioxide (As2O3) on human gastric cancer SGC-7901 cells. SGC-7901 cells were treated with different concentrations of As2O3 in the cell growth media for 24, 48 and 72 h, and the growth rates were determined by WST-1 cell proliferation assays. Analyses of nuclear morphological changes were performed with DAPI fluorescence staining. Cell apoptosis rates as assessed by flow cytometry were determined after cells were grown in media for 48 h containing different As2O3 concentrations. The protein expression patterns of the apoptosis factors, Bax, Fas and caspase-8, and anti-apoptosis factors, Akt, p-Akt, mTOR and p-mTOR, were evaluated by western blot analysis following treatment of the cells with different As2O3 concentrations in the cell growth media for 48 h. As a result, As2O3 inhibited the growth of human gastric cancer SGC-7901 cells in concentrations >5 µmol/l for longer than 24 h. Flow cytometric analysis revealed that the apoptosis of SGC-7901 cells occurred in an As2O3 concentration-dependent manner after 48 h (P<0.001). Expression levels of Bax, Fas and caspase-8 were increased, whereas expression levels of Akt, p-Akt, mTOR and p-mTOR were decreased in the SGC-7901 cells after a 48-h incubation with different As2O3 concentrations. In conclusion, As2O3 induced human gastric cancer SGC-7901 cell apoptosis in a time- and concentration dependent manner by inhibiting the activity of anti-apoptosis-related factors.
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CTL responses to HSP47 associated with the prolonged survival of patients with glioblastomas.
Neurology
PUBLISHED: 03-12-2014
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To define heat shock protein 47 (HSP47) as a novel glioma-associated antigen and to preliminarily assess the association of cytotoxic T lymphocyte (CTL) responses to HSP47 with clinical outcomes in patients with glioblastomas (GBMs).
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[CXCR4 and Nrf2 expressions in non-small cell lung cancer and their clinical implications].
Nan Fang Yi Ke Da Xue Xue Bao
PUBLISHED: 03-05-2014
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To detect the expressions of CXCR4 and Nrf2 in non-small cell lung cancer (NSCLC) tissues and analyze their association with the clinicopathological features of NSCLC.
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Solvents and auxiliary ligands co-regulate three antiferromagnetic Co(II) MOFs based on a semi-rigid carboxylate ligand.
Dalton Trans
PUBLISHED: 02-28-2014
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By reacting an asymmetry semi-rigid Y-shaped/L-shaped linker H3cpta (H3cpta = 3-(4'-carboxyphenoxy)phthalic acid) and Co(CH3COO)2·6H2O under different N-donor ligands in different solvents, three new Co-based coordination polymers, [Co3(cpta)2(bpe)3(H2O)4] (1) [Co(?2-H2O)(?3-OH)(Hcpta)(bpe)(H2O)·3(DMF)3(H2O)] (2) and [Co3(cpta)2(bpa)4] (3) have been obtained. They exhibit trinodal topological nets/layer, based on Co(2+) ions and Y-shaped/L-shaped carboxylate linkers. 1 and 3 present 3D frameworks with the point symbol {4·10(2)}2{10(5)·12}{4·8(5)}2 for 1 and {4·8(2)}2{8(5)·9}{4·6(7)·9(2)}2 for 3. While, 2 exhibits a 2D layer with the point symbol {4·6·8}{4·6(2)·8(3)}{6(2)·8}. The magnetic studies indicate that all of the three complexes show antiferromagnetic exchanges transmitted through ?3-carboxylate/?4-carboxylate bridges, ?2-H2O molecules and ?3-OH ions between Co(2+) ions, respectively. And the result of this research shows that the solvent and the secondary ligands could co-regulate coordination polymer with interesting properties, providing a constructive guidance when synthesizing versatile topologies with the same organic spacer but a different architecture.
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Facile synthesis of urchin-like NiCo2O4 hollow microspheres with enhanced electrochemical properties in energy and environmentally related applications.
ACS Appl Mater Interfaces
PUBLISHED: 02-26-2014
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A facile synthesis of novel urchin-like NiCo2O4 hierarchical hollow microspheres has been developed based on a template-free solvothermal and subsequent calcination method. The growth process of NiCo2O4 hollow microsphere precursors has been investigated, and a plausible mechanism was proposed. Because of their unique structure and high specific surface area, these NiCo2O4 hollow microspheres displayed enhanced electrochemical properties in methanol electrooxidation and determination of heavy-metal ions compared with solid urchin-like NiCo2O4 microspheres, Co3O4, and NiO microspheres. The good electrochemical performances suggested that these unique hierarchical NiCo2O4 hollow microspheres could be promising materials for energy and environmentally related applications.
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Post-stroke pain hypersensitivity induced by experimental thalamic hemorrhage in rats is region-specific and demonstrates limited efficacy of gabapentin.
Neurosci Bull
PUBLISHED: 02-20-2014
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Intractable central post-stroke pain (CPSP) is one of the most common sequelae of stroke, but has been inadequately studied to date. In this study, we first determined the relationship between the lesion site and changes in mechanical or thermal pain sensitivity in a rat CPSP model with experimental thalamic hemorrhage produced by unilateral intra-thalamic collagenase IV (ITC) injection. Then, we evaluated the efficacy of gabapentin (GBP), an anticonvulsant that binds the voltage-gated Ca(2+) channel ?2? and a commonly used anti-neuropathic pain medication. Histological case-by-case analysis showed that only lesions confined to the medial lemniscus and the ventroposterior lateral/medial nuclei of the thalamus and/or the posterior thalamic nucleus resulted in bilateral mechanical pain hypersensitivity. All of the animals displaying CPSP also had impaired motor coordination, while control rats with intra-thalamic saline developed no central pain or motor deficits. GBP had a dose-related anti-allodynic effect after a single administration (1, 10, or 100 mg/kg) on day 7 post-ITC, with significant effects lasting at least 5 h for the higher doses. However, repeated treatment, once a day for two weeks, resulted in complete loss of effectiveness (drug tolerance) at 10 mg/kg, while effectiveness remained at 100 mg/kg, although the time period of efficacious analgesia was reduced. In addition, GBP did not change the basal pain sensitivity and the motor impairment caused by the ITC lesion, suggesting selective action of GBP on the somatosensory system.
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Facile synthesis of porous single crystalline ZnO nanoplates and their application in photocatalytic reduction of Cr(VI) in the presence of phenol.
J. Hazard. Mater.
PUBLISHED: 02-19-2014
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Porous single crystalline ZnO nanoplates were successfully synthesized through a facile and cost-effective hydrothermal process at low temperature condition, followed by annealing of the zinc carbonate hydroxide hydrate precursors. The as-prepared products were characterized by X-ray diffraction (XRD), scanning electron microscopy (SEM), transmission electron microscopy (TEM), and Brunauer-Emmett-Teller (BET) measurements. The porous single crystalline ZnO nanoplates are with 12nm thickness and pore ranging from 10nm to several tens of nanometers. The porous structure of the ZnO nanoplates caused large amount of surface defects which worked as photogenerated holes' shallow trappers and largely restrained the recombination of photogenerated electrons and holes, resulting in a significantly high photocatalytic activity and durability toward the photoreduction of Cr(VI) under UV irradiation. Moreover, a synergistic effect, that is, increased photocatalytic reduction of Cr(VI) and degradation of phenol, can be observed. Furthermore, the synergistic photocatalytic mechanism has also been discussed. Those results present an enlightenment to employ porous single crystalline nanomaterials to remove Cr(VI) and organic pollutants simultaneously.
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miR-638 suppresses cell proliferation in gastric cancer by targeting Sp2.
Dig. Dis. Sci.
PUBLISHED: 02-19-2014
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MicroRNAs play important roles in the development and progression of various cancers. Recent studies have shown that miR-638 was downregulated in several tumors; however, its role in gastric cancer (GC) has not been investigated in detail.
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Establishment and maintenance of a standardized glioma tissue bank: Huashan experience.
Cell Tissue Bank
PUBLISHED: 02-14-2014
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Cerebral glioma is the most common brain tumor as well as one of the top ten malignant tumors in human beings. In spite of the great progress on chemotherapy and radiotherapy as well as the surgery strategies during the past decades, the mortality and morbidity are still high. One of the major challenges is to explore the pathogenesis and invasion of glioma at various "omics" levels (such as proteomics or genomics) and the clinical implications of biomarkers for diagnosis, prognosis or treatment of glioma patients. Establishment of a standardized tissue bank with high quality biospecimens annotated with clinical information is pivotal to the solution of these questions as well as the drug development process and translational research on glioma. Therefore, based on previous experience of tissue banks, standardized protocols for sample collection and storage were developed. We also developed two systems for glioma patient and sample management, a local database for medical records and a local image database for medical images. For future set-up of a regional biobank network in Shanghai, we also founded a centralized database for medical records. Hence we established a standardized glioma tissue bank with sufficient clinical data and medical images in Huashan Hospital. By September, 2013, tissues samples from 1,326 cases were collected. Histological diagnosis revealed that 73 % were astrocytic tumors, 17 % were oligodendroglial tumors, 2 % were oligoastrocytic tumors, 4 % were ependymal tumors and 4 % were other central nervous system neoplasms.
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Anion-coordination-induced turn-on fluorescence of an oligourea-functionalized tetraphenylethene in a wide concentration range.
Angew. Chem. Int. Ed. Engl.
PUBLISHED: 02-06-2014
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A tetrakis(bisurea)-decorated tetraphenylethene (TPE) ligand (L(2)) was designed, which, upon coordination with phosphate ions, displays fluorescence "turn-on" over a wide concentration range, from dilute to concentrated solutions and to the solid state. The fluorescence enhancement can be attributed to the restriction of the intramolecular rotation of TPE by anion coordination. The crystal structure of the A4L2 (A=anion) complex of L(2) with monohydrogen phosphate provides direct evidence for the coordination mode of the anion. This "anion-coordination-induced emission" (ACIE) is another approach for fluorescence turn-on in addition to aggregation-induced emission (AIE).
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High-throughput screening for developability during early-stage antibody discovery using self-interaction nanoparticle spectroscopy.
MAbs
PUBLISHED: 02-05-2014
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The discovery of monoclonal antibodies (mAbs) that bind to a particular molecular target is now regarded a routine exercise. However, the successful development of mAbs that (1) express well, (2) elicit a desirable biological effect upon binding, and (3) remain soluble and display low viscosity at high concentrations is often far more challenging. Therefore, high throughput screening assays that assess self-association and aggregation early in the selection process are likely to yield mAbs with superior biophysical properties. Here, we report an improved version of affinity-capture self-interaction nanoparticle spectroscopy (AC-SINS) that is capable of screening large panels of antibodies for their propensity to self-associate. AC-SINS is based on concentrating mAbs from dilute solutions around gold nanoparticles pre-coated with polyclonal capture (e.g., anti-Fc) antibodies. Interactions between immobilized mAbs lead to reduced inter-particle distances and increased plasmon wavelengths (wavelengths of maximum absorbance), which can be readily measured by optical means. This method is attractive because it is compatible with dilute and unpurified mAb solutions that are typical during early antibody discovery. In addition, we have improved multiple aspects of this assay for increased throughput and reproducibility. A data set comprising over 400 mAbs suggests that our modified assay yields self-interaction measurements that are well-correlated with other lower throughput assays such as cross-interaction chromatography. We expect that the simplicity and throughput of our improved AC-SINS method will lead to improved selection of mAbs with excellent biophysical properties during early antibody discovery.
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Lineage and species-specific long noncoding RNAs during erythro-megakaryocytic development.
Blood
PUBLISHED: 02-04-2014
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Mammals express thousands of long noncoding (lnc) RNAs, a few of which are known to function in tissue development. However, the entire repertoire of lncRNAs in most tissues and species is not defined. Indeed, most lncRNAs are not conserved, raising questions about function. We used RNA sequencing to identify 1109 polyadenylated lncRNAs expressed in erythroblasts, megakaryocytes, and megakaryocyte-erythroid precursors of mice, and 594 in erythroblasts of humans. More than half of these lncRNAs were unannotated, emphasizing the opportunity for new discovery through studies of specialized cell types. Analysis of the mouse erythro-megakaryocytic polyadenylated lncRNA transcriptome indicates that ~75% arise from promoters and 25% from enhancers, many of which are regulated by key transcription factors including GATA1 and TAL1. Erythroid lncRNA expression is largely conserved among 8 different mouse strains, yet only 15% of mouse lncRNAs are expressed in humans and vice versa, reflecting dramatic species-specificity. RNA interference assays of 21 abundant erythroid-specific murine lncRNAs in primary mouse erythroid precursors identified 7 whose knockdown inhibited terminal erythroid maturation. At least 6 of these 7 functional lncRNAs have no detectable expression in human erythroblasts, suggesting that lack of conservation between mammalian species does not predict lack of function.
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TERT promoter mutations contribute to subset prognostication of lower-grade gliomas.
Mod. Pathol.
PUBLISHED: 01-27-2014
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Recurrent mutations in the promoter region of telomerase reverse transcriptase (TERT) have been found in various cancers including diffuse gliomas. Mutations lead to TERT upregulation and are associated with aggressive clinical behavior in glioblastomas. However, the clinical significance of TERT promoter mutations in lower-grade gliomas remains undetermined. The aim of this study is to evaluate the status of TERT promoter and the respective prognostic significance in a cohort of 237 lower-grade gliomas comprising grades II and III astrocytomas, oligodendrogliomas, and oligoastrocytomas. Mutually exclusive mutations in TERT promoter, C228T and C250T, were identified in 16/105 (15%) diffuse astrocytomas, 16/63 (25%) anaplastic astrocytomas, 13/18 (72%) oligodendrogliomas, 3/3 (100%) anaplastic oligodendrogliomas, 17/45 (38%) oligoastrocytomas, and 2/3 (67%) anaplastic oligoastrocytomas. Mutations co-occurred with 1p/19q codeletion (P<0.001) and are associated with oligodendroglial histology (P<0.001). Kaplan-Meier's survival analysis showed that TERT promoter mutation (P=0.037), Isocitrate dehydrogenase (IDH) mutation (P<0.001), and 1p/19q codeletion (P<0.001) were associated with favorable overall survival (OS). In the subset of 116 IDH-mutated lower-grade gliomas lacking 1p/19q codeletion, 19 TERT promoter-mutated tumors exhibited longer progression-free survival (PFS) (P=0.027) and OS (P=0.004). Consistent with this observation, in the subset of 97 IDH-mutated astrocytomas, 14 TERT promoter-mutated tumors showed longer PFS (P=0.001) and OS (P=0.001). In contrast, among the subset of 74 IDH wild-type lower-grade gliomas with intact 1p/19q, TERT promoter mutation was associated with shorter PFS (P=0.001) and OS (P=0.001). Similarly, in the subset of 65 IDH wild-type astrocytomas, 16 TERT promoter-mutated tumors exhibited unfavorable PFS (P=0.007) and OS (P=0.008). Our results indicate that when combined with IDH status, TERT promoter mutation contributes to prognostic subgroups of lower-grade astrocytic tumors or 1p/19q intact lower-grade gliomas and this may further refine future molecular classification of lower-grade gliomas.Modern Pathology advance online publication, 1 August 2014; doi:10.1038/modpathol.2014.94.
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Relationship between hepatitis B virus genotype B and C and response to interferon therapy in HBeAg positive chronic hepatitis B patients: A meta-analysis.
J. Gastroenterol. Hepatol.
PUBLISHED: 01-27-2014
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Previous studies examining the relationship between hepatitis B virus (HBV) genotype B and C and response to interferon therapy in Hepatitis B e antigen (HBeAg)-positive chronic hepatitis B patients have yielded conflicting results. We aim to summarize data to reach firm conclusions on the role of HBV genotype B and C in response to interferon therapy.
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Copper-promoted oxidative coupling of enamides and alkynes for the synthesis of substituted pyrroles.
Chemistry
PUBLISHED: 01-22-2014
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An efficient copper-promoted oxidative coupling of enamides with alkynes has been developed for the synthesis of substituted pyrroles. The reaction proceeded through C-H and N-H bond functionalization of enamides under mild conditions.
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Proteomic analysis of cerebrospinal fluid: toward the identification of biomarkers for gliomas.
Neurosurg Rev
PUBLISHED: 01-19-2014
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Gliomas are the most common primary brain tumors in adults and, despite advances in the understandings of glioma pathogenesis in the genetic era, they are still ineradicable, justifying the need to develop more reliable diagnostic and prognostic biomarkers for this malignancy. Because changes in cerebrospinal fluid (CSF) are suggested to be capable of sensitively reflecting pathological processes, e.g., neoplastic conditions, in the central nervous system, CSF has been deemed a valuable source for potential biomarkers screening in this era of proteomics. This systematic review focused on the proteomic analysis of glioma CSF that has been published to date and identified a total of 19 differentially expressed proteins. Further functional and protein-protein interaction assessments were performed by using Protein Analysis Through Evolutionary Relationships (PANTHER) website and Ingenuity Pathway Analysis (IPA) software, which revealed several important protein networks (e.g., IL-6/STAT-3) and four novel focus proteins (IL-6, galanin (GAL), HSPA5, and WNT4) that might be involved in glioma pathogenesis. The concentrations of these focus proteins were subsequently determined by enzyme-linked immunosorbent assay (ELISA) in an independent set of CSF and tumor cyst fluid (CF) samples. Specifically, glioblastoma (GBM) CF had significantly lower GAL, HSPA5, and WNT4 levels than CSF from different grades of glioma. In contrast, IL-6 level was significantly higher in GBM CF when compared with CSF and, among different CSF groups, was highest in GBM CSF. Therefore, these candidate protein biomarkers, identified from both the literatures and in silico analysis, may have potentials in clinical diagnosis, prognosis evaluation, treatment response monitoring, and novel therapeutic targets identification for patients with glioma.
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Evidence of brain tumor stem progenitor-like cells with low proliferative capacity in human benign pituitary adenoma.
Cancer Lett.
PUBLISHED: 01-19-2014
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Tumor stem cells have been implicated as cancer-initiating cells in malignant brain tumors. However, whether benign brain tumors also contain tumor stem cells are largely unexplored. Here, we investigated whether tumor stem-like cells were present in pituitary adenoma similar to malignant brain tumors. By immunocytochemistry, we found that pituitary adenoma tissues expressed neural stem cell marker. These cells could form neurospheres in vitro, expressed neural stem/progenitor cell markers and generated daughter cells with the capacity to differentiate into three neural lineages. Importantly, compared with non-invasive pituitary adenomas, we found that CD133 expression was significantly increased in invasive pituitary adenomas, suggesting that the proliferative capacity was correlated with the malignance of pituitary adenomas. Finally, invasive pituitary adenomas cells displayed lower proliferative ability than glioblastoma. Our data indicate that a subpopulation of stem/progenitor-like cells are present in pituitary adenomas, and these cells may be responsible for benign tumor initiation and maintenance.
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Increased expression of Capn4 is associated with the malignancy of human glioma.
CNS Neurosci Ther
PUBLISHED: 01-19-2014
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Recent evidence indicates that the increased expression of calpain small subunit 1 (Capn4) is associated with tumorigenesis. This study was designed to explore the role which Capn4 plays in human glioma.
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Model-based verification of a non-linear separation scheme for ballistocardiography.
IEEE J Biomed Health Inform
PUBLISHED: 01-10-2014
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The current rise in popularity of ballisto-cardiography-related research has led to the development of new sensor concepts and recording methods. Measuring the ballistocardiogram using bed mounted pressure sensors opens up new possibilities for home monitoring applications. The signals measured with these sensors contain a mixture of cardiac and respiratory components, which can be used for detection of comorbidities of heart failure like apnea or arrhythmia. However, the separation of the cardiac and respiratory components has proven to be difficult, since there is significant overlap in the spectra of both components. In this paper, an algorithm for the separation task is presented, which can overcome the problem of overlapping spectra. Additionally, a model has been developed for the generation of artificial ballistocardiograms, which are used to analyze the separation performance. Furthermore, the algorithm is tested on preliminary data from a clinical study.
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Pd-catalyzed oxidative coupling of enamides and alkynes for synthesis of substituted pyrroles.
Org. Lett.
PUBLISHED: 01-09-2014
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A novel and efficient palladium(II)-catalyzed alkenyl C-H activation oxidative annulation of enamides with alkynes for the synthesis of substituted pyrroles has been developed. The reaction tolerates a wide range of functional groups and is a reliable method for the synthesis of triaryl-substituted pyrroles in high yields.
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Mouse glioma immunotherapy mediated by A2B5+ GL261 cell lysate-pulsed dendritic cells.
J. Neurooncol.
PUBLISHED: 01-08-2014
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Immunotherapy strategies targeting glioma stem-like cells (GSCs) hold promise for improving outcomes in glioblastoma patients. We used the A2B5 monoclonal antibody to classify GSCs derived from the mouse GL261 glioma cell line, and A2B5+ GL261 cell lysate-pulsed dendritic cells (DCs) were used to treat mouse glioma. We found that such DCs elicited a stronger specific cytotoxic T lymphocyte response against A2B5+ GL261 cells than A2B5- GL261 cell lysate-pulsed DCs. The effect of A2B5+ GL261 cell lysate-pulsed DCs in vivo depended on when the vaccination was started. In the tumor cell adaptation phase, C57BL/6 mice had an immune response against GL261, and vaccination enhanced the immune response and prevented glioma formation in 37.5% of mice. In contrast, after glioma formation, the immune response against GL261 was decreased, and vaccination had no therapeutic effect. Our findings suggest that vaccination with A2B5+ GL261 cell lysate-pulsed DCs only has some glioma preventive effect.
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Myeloid mineralocorticoid receptor deficiency inhibits aortic constriction-induced cardiac hypertrophy in mice.
PLoS ONE
PUBLISHED: 01-01-2014
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Mineralocorticoid receptor (MR) blockade has been shown to suppress cardiac hypertrophy and remodeling in animal models of pressure overload (POL). This study aims to determine whether MR deficiency in myeloid cells modulates aortic constriction-induced cardiovascular injuries. Myeloid MR knockout (MMRKO) mice and littermate control mice were subjected to abdominal aortic constriction (AAC) or sham operation. We found that AAC-induced cardiac hypertrophy and fibrosis were significantly attenuated in MMRKO mice. Expression of genes important in generating reactive oxygen species was decreased in MMRKO mice, while that of manganese superoxide dismutase increased. Furthermore, expression of genes important in cardiac metabolism was increased in MMRKO hearts. Macrophage infiltration in the heart was inhibited and expression of inflammatory genes was decreased in MMRKO mice. In addition, aortic fibrosis and inflammation were attenuated in MMRKO mice. Taken together, our data indicated that MR deficiency in myeloid cells effectively attenuated aortic constriction-induced cardiac hypertrophy and fibrosis, as well as aortic fibrosis and inflammation.
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Utilizing FMR1 gene mutations as predictors of treatment success in human in vitro fertilization.
PLoS ONE
PUBLISHED: 01-01-2014
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Mutations of the fragile X mental retardation 1 (FMR1) gene are associated with distinct ovarian aging patterns.
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Medulloblastoma in China: clinicopathologic analyses of SHH, WNT, and non-SHH/WNT molecular subgroups reveal different therapeutic responses to adjuvant chemotherapy.
PLoS ONE
PUBLISHED: 01-01-2014
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Medulloblastoma (MB) is one of the most common primary central nervous system tumors in children. Data is lacking of a large cohort of medulloblastoma patients in China. Also, our knowledge on the sensitivity of different molecular subgroups of MB to adjuvant radiation therapy (RT) or chemotherapy (CHT) is still limited. The authors performed a retrospective study of 173 medulloblastoma patients treated at two institutions from 2002 to 2011. Formalin-fixed paraffin embedded (FFPE) tissues were available in all the cases and sections were stained to classify histological and molecular subgroups. Univariate and multivariate analyses were used to investigate prognostic factors. Of 173 patients, there were 118 children and 55 adults, 112 males and 61 females. Estimated 5-year overall survival (OS) rates for all patients, children and adults were 52%, 48% and 63%, respectively. After multivariate analysis, postoperative primary radiation therapy (RT) and chemotherapy (CHT) were revealed as favorable prognostic factors influencing OS and EFS. Postoperative primary chemotherapy (CHT) was found significantly improving the survival of children (p<0.001) while it was not a significant prognostic factor for adult patients. Moreover, patients in WNT subtype had better OS (p?=?0.028) than others (SHH and Non-SHH/WNT subtypes) given postoperative adjuvant therapies. Postoperative primary RT was found to be a strong prognostic factor influencing the survival in all histological and molecular subgroups (p<0.001). Postoperative primary CHT was found significantly to influence the survival of classic medulloblastoma (CMB) (OS p<0.001, EFS p<0.001), SHH subgroup (OS p?=?0.020, EFS p?=?0.049) and WNT subgroup (OS p?=?0.003, EFS p?=?0.016) but not in desmoplastic/nodular medulloblastoma (DMB) (OS p?=?0.361, EFS p?=?0.834) and Non-SHH/WNT subgroup (OS p?=?0.127, EFS p?=?0.055). Our study showed postoperative primary CHT significantly influence the survival of CMB, SHH subgroup and WNT subgroup but not in DMB and Non-SHH/WNT subgroup of MB.
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Wide Wavelength Tuning of Optical Antennas on Graphene with Nanosecond Response Time.
Nano Lett.
PUBLISHED: 12-06-2013
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Graphene is emerging as a broadband optical material which can be dynamically tuned by electrostatic doping. However, the direct application of graphene sheets in optoelectronic devices is challenging due to graphenes small thickness and the resultant weak interaction with light. By combining metal and graphene in a hybrid plasmonic structure, it is possible to enhance graphene-light interaction and thus achieve in situ control of the optical response. We show that the effective mode index of the bonding plasmonic mode in metal-insulator-metal (MIM) waveguides is particularly sensitive to the change in the optical conductivity of a graphene layer in the gap. By incorporating such MIM structures in optic antenna designs, we demonstrate an electrically tunable coupled antenna array on graphene with a large tuning range (1100 nm, i.e., 250 cm(-1), nearly 20% of the resonance frequency) of the antenna resonance wavelength at the mid-infrared (MIR) region. Our device exhibits a 3 dB cutoff frequency of 30 MHz, which can be further increased into the gigahertz range. This study confirms that hybrid metal-graphene structures are promising elements for high-speed electrically controllable optical and optoelectronic devices.
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Solvent or temperature induced diverse coordination polymers of silver(i) sulfate and bipyrazole systems: syntheses, crystal structures, luminescence, and sorption properties.
Inorg Chem
PUBLISHED: 12-02-2013
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Three new coordination polymers, [Ag4(H2bpz)4(SO4)2]·H2O (1), [Ag2(H2bpz)2(SO4)]·3H2O (2), and [Ag3(H2bpz)4](SO4)2/3(OH)5/3·4H2O (3) have been solvothermally synthesized with Ag2SO4 and flexible ligand 3,3,5,5-tetramethyl-4,4-bipyrazole (H2bpz) in different solvents and temperatures. Complex 1 is a 2-fold interpenetrated three-dimensional (3D) framework with an uncommon (3,5)-connected hms topology. Complex 2 is a structural isomer of 1 and shows a three-connected 2D ths net consisting of interesting 3-fold and 2-fold heterochiral helical chains. Complex 3 discloses a grid layer structure, containing heterochiral helical chains and an unusual meso-helix. More interestingly, three sets of layers in 3 stack in different directions, affording an unprecedented 2D + 2D + 2D ? 3D polycatenating cationic framework with 1D + 3D porous systems. In 1-3, H2bpz exhibit exobidentate bridging fashions with wide-ranged interpyrazole tilting angles and changeable coordination configurations, such as cis and trans fashions in 1 and 3 and uniform trans fashion in 2. These lead to the isomeric [Ag(H2bpz)]n arrays of wavelike and helical chains in 1 and 2, respectively. Complexes 1-3 display solid-state photoluminescence stemming from the ligand-centered fluorescent emissions of H2bpz. Because of the highly polar framework, 3 shows excellent adsorption selectivity for CO2 over N2.
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Necklace-like mesoporous MgO/TiO2 heterojunction structures with excellent capability for water treatment.
Dalton Trans
PUBLISHED: 12-02-2013
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Necklace-like MgO/TiO2 heterojunction structures were synthesized by a simple and economical precursor calcination method for the first time. The obtained unique MgO/TiO2 heterojunction structures exhibit an excellent removal performance towards toxic As(v) ions combined with good photocatalytic properties towards organic dyes.
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Serological Identification of URGCP as a Potential Biomarker for Glioma.
CNS Neurosci Ther
PUBLISHED: 10-26-2013
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Glioblastoma multiforme (GBM) is one of the most frequent human brain tumor and causes dismal outcome. To identify tumor-associated antigens in GBM patients may find potential diagnostic markers and immunotherapeutic targets. In this study, we identified a gene termed URGCP using the serological identification of antigens by recombinant A2B5 positive glioma cDNA library. The gene product of URGCP is immunogenic in GBM after tested in allogenic patients serum screening.
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Palladium-Catalyzed Oxidative Carbonylation of the Alkenyl C?H Bonds of Enamides: Synthesis of 1,3-Oxazin-6-ones.
Angew. Chem. Int. Ed. Engl.
PUBLISHED: 09-10-2013
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Palladium and CO.: The title reaction proceeds in the presence of CO, thus providing a synthesis for 1,3-oxazin-6-ones (see scheme; DABCO=1,4-diazabicyclo[2.2.2]octane, DMF=N,N-dimethylformamide). The reaction tolerates a variety of functional groups on both the aryl ring and the amide of the substrate. Initial mechanistic studies suggest the activation of the alkenyl C?H bond to be a key step.
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Cu(TFA)2-catalyzed oxidative tandem cyclization/1,2-alkyl migration of enamino amides for synthesis of pyrrolin-4-ones.
Org. Lett.
PUBLISHED: 08-29-2013
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A novel Cu(TFA)2-catalyzed oxidative tandem cyclization/1,2-alkyl migration of readily available enamino amides for the synthesis of pyrrolin-4-ones has been developed. The reaction tolerates a wide range of functional groups and is a reliable method for the rapid synthesis of substituted pyrrolin-4-ones in high yields under mild conditions.
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Clonal genetic and hematopoietic heterogeneity among human-induced pluripotent stem cell lines.
Blood
PUBLISHED: 08-12-2013
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Induced pluripotent stem cells (iPSCs) hold great promise for modeling human hematopoietic diseases. However, intrinsic variability in the capacities of different iPSC lines for hematopoietic development complicates comparative studies and is currently unexplained. We created and analyzed 3 separate iPSC clones from fibroblasts of 3 different normal individuals using a standardized approach that included excision of integrated reprogramming genes by Cre-Lox mediated recombination. Gene expression profiling and hematopoietic differentiation assays showed that independent lines from the same individual were generally more similar to one another than those from different individuals. However, one iPSC line (WT2.1) exhibited a distinctly different gene expression, proliferation rate, and hematopoietic developmental potential relative to all other iPSC lines. This "outlier" clone also acquired extensive copy number variations (CNVs) during reprogramming, which may be responsible for its divergent properties. Our data indicate how inherent and acquired genetic differences can influence iPSC properties, including hematopoietic potential.
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Oxidation of 2-arylindoles for synthesis of 2-arylbenzoxazinones with oxone as the sole oxidant.
Chem. Commun. (Camb.)
PUBLISHED: 08-09-2013
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A novel and efficient method for the oxidation of 2-arylindoles to synthesize 2-arylbenzoxazinones utilizing oxone as the sole oxidant has been developed. The reaction tolerates a wide range of functional groups and allows quick and atom-economical assembly of a variety of valuable 2-arylbenzoxazinones in high yields.
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What is Visualize?

JoVE Visualize is a tool created to match the last 5 years of PubMed publications to methods in JoVE's video library.

How does it work?

We use abstracts found on PubMed and match them to JoVE videos to create a list of 10 to 30 related methods videos.

Video X seems to be unrelated to Abstract Y...

In developing our video relationships, we compare around 5 million PubMed articles to our library of over 4,500 methods videos. In some cases the language used in the PubMed abstracts makes matching that content to a JoVE video difficult. In other cases, there happens not to be any content in our video library that is relevant to the topic of a given abstract. In these cases, our algorithms are trying their best to display videos with relevant content, which can sometimes result in matched videos with only a slight relation.