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Find video protocols related to scientific articles indexed in Pubmed.
Baohuoside I suppresses invasion of cervical and breast cancer cells through the downregulation of CXCR 4 chemokine receptor expression.
Biochemistry
PUBLISHED: 11-20-2014
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Over ninety percent of cancer-mediated deaths are due to metastasis, but the mechanisms that control metastasis remain poorly understood. Thus, the therapy targeting this process has been challenged constantly but no therapy has yet been approved. The CXC chemokine receptor 4 (CXCR4), a Gi protein coupled receptor for the CXC chemokine ligand (CXCL) 12 /stromal cell derived factor(SDF)-1?, is known to be expressed in various tumors. Recently, the CXCL12/CXCR4 axis has emerged as a key mediator of tumor metastasis, therefore it has been considered that identification of CXCR4 inhibitors can be a promising strategy for abrogating metastasis. In the present report, we investigated baohuoside I, a component of Epimedium koreanum, as a regulator of CXCR4 expression as well as function in cervical cancer and breast cancer cells. We observed that baohuoside I downregulated CXCR4 expression in a dose- and time-dependent manner in HeLa cells. Treatment with a pharmacological proteasome and lysosomal inhibitors did not have a substantial effect on baohuoside I's ability to decrease CXCR4 expression. When we investigated the molecular mechanism of action, it was observed that the suppression of CXCR4 expression occurred at the level of mRNA. Decrease of CXCR4 expression by baohuoside I was correlated with inhibition of the CXCL12-induced invasion of both cervical and breast cancer cells. Overall, our results show that baohuoside I exerts its anti-metastatic effect through the downregulation of CXCR4 expression, and thus, has a potential to play a role in the suppression of cancer metastasis.
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Highly efficient deep-blue phosphorescence from heptafluoropropyl-substituted iridium complexes.
Chem. Commun. (Camb.)
PUBLISHED: 11-20-2014
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New deep-blue iridium complexes, consisting of a heptafluoropropyl (HFP) substituent at the 3' position of 2',4''-difluorophenyl, have a deep HOMO level and decreased shoulder electronic transition and inhibit self-quenching due to the sterically hindered group without conjugation. An OLED using (HFP)2Ir(mpic) exhibited a maximum EQE of 21.4% with a CIE of (0.146, 0.165).
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Patient-Clinician Mobile Communication: Analyzing Text Messaging between Adolescents with Asthma and Nurse Case Managers.
Telemed J E Health
PUBLISHED: 11-18-2014
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Abstract Background: With the increasing penetration of digital mobile devices among adolescents, mobile texting messaging is emerging as a new channel for patient-clinician communication for this population. In particular, it can promote active communication between healthcare clinicians and adolescents with asthma. However, little is known about the content of the messages exchanged in medical encounters via mobile text messaging. Therefore, this study explored the content of text messaging between clinicians and adolescents with asthma. Materials and Methods: We collected a total of 2,953 text messages exchanged between 5 nurse case managers and 131 adolescents with asthma through a personal digital assistant. The text messages were coded using a scheme developed by adapting categories from the Roter Interaction Analysis System. Results: Nurse case managers sent more text messages (n=2,639) than adolescents with asthma. Most messages sent by nurse case managers were targeted messages (n=2,475) directed at all adolescents with asthma, whereas there were relatively few tailored messages (n=164) that were created personally for an individual adolescent. In addition, both targeted and tailored messages emphasized task-focused behaviors over socioemotional behaviors. Likewise, text messages (n=314) sent by adolescents also emphasized task-focused over socioemotional behaviors. Conclusions: Mobile texting messaging has the potential to play an important role in patient-clinician communication. It promotes not only active interaction, but also patient-centered communication with clinicians. In order to achieve this potential, healthcare clinicians may need to focus on socioemotional communication as well as task-oriented communication.
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Organic Field-Effect Transistors: Dramatic Inversion of Charge Polarity in Diketopyrrolopyrrole-Based Organic Field-Effect Transistors via a Simple Nitrile Group Substitution (Adv. Mater. 43/2014).
Adv. Mater. Weinheim
PUBLISHED: 11-14-2014
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A record-breaking high electron mobility (up to 7.0 cm(2) V(-1) s(-1) ) is demonstrated with a diketopyrrolopyrrole (DPP)-based semiconductor (PDPP-CNTVT), of which a vinylene unit is simply incorporated with only one nitrile group, as described by Y.-H. Kim, Y.-Y. Noh, as described by S.-K. Kwon, and co-workers on page 7300. Both the strong electron-withdrawing property of the nitrile group and its low steric-hindrance effect in the conjugation backbone can help the hole-dominant polymer (PDPP-TVT) to be converted dramatically into an outstanding electron-dominant semiconductor.
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Apolipoprotein E4 Affects Topographical Changes in Hippocampal and Cortical Atrophy in Alzheimer's Disease Dementia: A Five-Year Longitudinal Study.
J. Alzheimers Dis.
PUBLISHED: 11-14-2014
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Apolipoprotein E4 (APOE4) is a genetic risk factor for developing Alzheimer's disease (AD). Once AD manifests clinically, however, the effects of APOE4 are less clear. Therefore, we investigated the longitudinal effects of APOE4 on topographical changes in AD patient brain atrophy. We prospectively recruited 35 patients with AD (19 APOE4 carriers and 16 non-carriers), and 14 normal controls, then followed them for five years. We measured hippocampal deformities and cortical thickness. Hippocampal comparison between APOE4 carriers and non-carriers with AD showed carriers had rapid changes in the head and body, while non-carriers had rapid changes in a small portion of the body. Cortical thickness comparison between APOE4 carriers and non-carriers with AD dementia showed carriers had rapid thinning in the lateral frontal, temporal, and parietal regions, while no region showed more rapid cortical thinning in non-carriers than in carriers. These findings underlined the importance of the APOE4 allele for designing and interpreting future treatment trials in patients with AD dementia.
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Mutagenicity and tumor-promoting effects of Tiglium seed extract via PKC and MAPK signaling pathways.
Biosci. Biotechnol. Biochem.
PUBLISHED: 11-14-2014
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Tiglium seed is a seed of mature Croton Tiglium Linne containing croton oils, which have been traditionally used as laxative or purgative. As it contains phorbol derivatives, we investigated the mutagenicity and tumor-promoting activity of Tiglium seed. Tiglium seed extract produced the mutagenic responses in five Salmonella typhimurium strains in Ames assay, whereas it did not alter the frequencies of chromosomal aberrations or micronuclei, indicating that it exerted the mutagenic potential, not clastogenicity. Accompanied with phosphorylation of connexin43 (Cx43) and extracellular signal-regulated kinases 1/2 (ERK1/2), Tiglium seed extract inhibited gap junctional intercellular communication (GJIC) associated with tumor-promoting potential. Importantly, these effects were blocked by a protein kinase C (PKC) inhibitor or mitogen-activated protein kinase (MAPKs) inhibitors, suggesting that Tiglium seed-induced GJIC inhibition was regulated by phosphorylation of Cx43 via PKC and MAPKs signaling. In conclusion, Tiglium seed has mutagenicity, possibly linking to tumor-promoting potential through the dysfunction of GJIC.
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Effect of Goniosynechialysis During Phacoemulsification on IOP in Patients With Medically Well-controlled Chronic Angle-Closure Glaucoma.
J. Glaucoma
PUBLISHED: 11-12-2014
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To evaluate and compare the efficacy and safety of combined phacoemulsification and goniosynechialysis (PEGS) to phacoemulsification alone (PE) in patients with medically well-controlled chronic angle-closure glaucoma (CACG) with cataracts.
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A tegument-specific venom allergen-like protein of Clonorchis sinensis.
Parasitol. Res.
PUBLISHED: 10-24-2014
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Venom allergen-like (VAL) proteins, members of the SCP/TAPS (sperm coating protein/Tpx-1/Ag5/PR-1/Sc7) protein superfamily, have been reported from several parasitic helminths. As little is known about their biological functions, a VAL protein of the Chinese liver fluke Clonorchis sinensis was cloned and characterized. A complementary DNA (cDNA) encoding a 25-kDa protein was identified from an EST database of C. sinensis. A BLAST search revealed that the protein shares 46 % sequence identity with Schistosoma mansoni VAL 13 protein, and thus, the protein was named CsVAL13. Multiple sequence alignment indicated that the SCP/TAPS domain of CsVAL13 shares 39-46 % sequence identity with VAL proteins from parasitic helminths. His and Tyr, which help to stabilize protein structure, were highly conserved across the VAL protein sequences. Phylogenetic analysis showed that the SCP/TAPS domain of the CsVAL13 sequence clusters together with other group 2 VAL protein sequences. In the homology-modeled structure of CsVAL13, an ?-?-? sandwich and residues for a putative active site were highly conserved. Immune sera were obtained from BALB/c mice immunized with the recombinant CsVAL13 protein. Immunohistochemical localization using the immune sera revealed that CsVAL13 was distributed mainly in the tegument and intrauterine eggs of adult C. sinensis. These findings suggest that CsVAL13 may be involved in host-parasite interactions and immune stimulation on the surrounding host environments.
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The Effect of Dexmedetomidine on Intraocular Pressure Increase in Patients During Robot-Assisted Laparoscopic Radical Prostatectomy in the Steep Trendelenburg Position.
J. Endourol.
PUBLISHED: 10-14-2014
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Abstract Purpose: This study was to evaluate the effect of intraoperative continuous infusion of dexmedetomidine on intraocular pressure (IOP) in patients undergoing robot-assisted laparoscopic radical prostatectomy (RALRP) in the steep Trendelenburg (ST). Materials and Methods: Sixty-eight patients were randomly divided into two groups. The dexmedetomidine group (Group D, n=34) received a continuous infusion of dexmedetomidine at a rate of 0.4??g kg(-1) hour(-1) from the induction of anesthesia until the end of the ST position, while the control group (Group C, n=34) received an equal volume of physiologic saline at the same rate under conventional general anesthesia with sevoflurane and remifentanil. IOP was measured at 11 predefined time points for all patients. Results: Significant differences in IOP were detected between the two groups by a linear mixed model analysis (p<0.001). The highest mean IOP was 19.9±5.0?mm Hg in Group D and 25.7±5.0?mm Hg in Group C; both were measured 60 minutes after the patients had been placed in the ST position. No significant between-group differences in ocular perfusion pressure, mean blood pressure, or heart rate were observed between the two groups. No ocular or other complications were noted. Conclusion: Intraoperative continuous infusion of dexmedetomidine may help alleviate IOP increase in patients undergoing RALRP in the ST position.
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Random copolymers: novel diketopyrroloppyrrole random copolymers: high charge-carrier mobility from environmentally benign processing (adv. Mater. 38/2014).
Adv. Mater. Weinheim
PUBLISHED: 10-11-2014
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On page 6612, D. S. Chung, Y.-H. Kim, S.-K. Kwon, and co-workers demonstrate a smart molecular-design strategy of polymer semiconductors to achieve their processability in environmentally benign solvents. Random copolymerization between two different DPP-based repeating units enables the polymer to be soluble in a wide range of green solvents including tetralin, and also leads to excellent charge-transport characteristics with a charge-carrier mobility of >5 cm(2) V(-1) s(-1) .
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RING Finger Protein RNF207: A Novel Regulator of Cardiac Excitation.
J. Biol. Chem.
PUBLISHED: 10-05-2014
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Two recent studies [Newton-Cheh et al., Nature Genetics 41, 399-406 (2009); Pfeufer et al., Nature Genetics 41, 407-414 (2009)] identified an association, with genome-wide significance, between a single nucleotide polymorphism within the gene encoding RING finger protein 207 (RNF207) and the QT interval. We sought to determine the role of RNF207 in cardiac electrophysiology. Morpholino knockdown of RNF207 in zebrafish embryos resulted in APD prolongation, occasionally a 2:1 atrioventricular block and slowing of conduction velocity. Conversely, neonatal rabbit cardiomyocytes infected with RNF207-expressing adenovirus exhibited shortened APD. Using transfections of U-2 OS and HEK293 cells, western blot and immunocytochemistry data demonstrate that RNF207 and the HERG (human ether-a-go-go-related gene) potassium channel interact and co-localize. Furthermore, RNF207 overexpression significantly elevated total and membrane HERG protein and HERG-encoded current density by approximately 30-50%, which was dependent on the intact N-terminal RING domain of RNF207. Finally, co-expression of RNF207 and HSP70 increased HERG expression as compared to HSP70 alone. This effect was dependent on the C-terminus of RNF207. Taken together, the evidence is strong that RNF207 is an important regulator of action potential duration likely via effects on HERG trafficking and localization in a heat shock protein-dependent manner.
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Visual Fields and OCT Role in Diagnosis of Glaucoma.
Optom Vis Sci
PUBLISHED: 10-04-2014
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To evaluate the clinical performance of visual field (VF) tests and optical coherence tomography (OCT) in diagnosing glaucoma.
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A Hierarchical Cluster Analysis of Normal-Tension Glaucoma Using Spectral-Domain Optical Coherence Tomography Parameters.
J. Glaucoma
PUBLISHED: 09-30-2014
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Normal-tension glaucoma (NTG) is a heterogenous disease, and there is still controversy about subclassifications of this disorder. On the basis of spectral-domain optical coherence tomography (SD-OCT), we subdivided NTG with hierarchical cluster analysis using optic nerve head (ONH) parameters and retinal nerve fiber layer (RNFL) thicknesses.
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Progesterone modulates SERCA2a expression and function in rabbit cardiomyocytes.
Am. J. Physiol., Cell Physiol.
PUBLISHED: 09-26-2014
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Background: We recently showed that progesterone treatment abolished arrhythmias and sudden cardiac death in a transgenic rabbit model of long QT syndrome type 2 (LQT2). Moreover, levels of cardiac sarcoplasmic reticulum Ca(2)(+) -ATPase2a (SERCA2a) were upregulated in LQT2 heart extracts. We hypothesized that progesterone treatment upregulated SERCA2a expression, thereby reducing Ca(2+)-dependent arrhythmias in LQT2 rabbits. We therefore investigated the effect of progesterone on SERCA2a regulation in isolated cardiomyocytes. Materials & methods: Cardiomyocytes from neonate rabbits (3-5 days old) were isolated, cultured, and treated with progesterone and other pharmacological agents. Immunoblotting was performed on total cell lysates and SR-enriched membrane fractions for protein abundance, and mRNA transcripts were quantified using real-time PCR. The effect of progesterone on baseline Ca(2+) transients and Ca(2+) clearance was determined using digital imaging. Results: Progesterone treatment increased the total pool of SERCA2a protein by slowing its degradation. Using various pharmacological inhibitors of degradation pathways, we showed that progesterone-associated degradation of SERCA2a involves ubiquitination, and progesterone significantly decreases the levels of ubiquitin-tagged SERCA2a polypeptides. Our digital imaging data revealed that progesterone significantly shortened the decay and duration of Ca(2+) transients. Conclusion: Progesterone treatment increases protein levels and activity of SERCA2a. Progesterone stabilizes SERCA2a, in part, by decreasing the ubiquitination level of SERCA2a polypeptides.
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Effects of cyano-substituents on the molecular packing structures of conjugated polymers for bulk-heterojunction solar cells.
ACS Appl Mater Interfaces
PUBLISHED: 09-08-2014
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The molecular packing structures of two conjugated polymers based on alkoxy naphthalene, one with cyano-substituents and one without, have been investigated to determine the effects of electron-withdrawing cyano-groups on the performance of bulk-heterojunction solar cells. The substituted cyano-groups facilitate the self-assembly of the polymer chains, and the cyano-substituted polymer:PC71BM blend exhibits enhanced exciton dissociation to PC71BM. Moreover, the electron-withdrawing cyano-groups lower the highest occupied molecular orbital (HOMO) and lowest unoccupied molecular orbital (LUMO) levels of the conjugated polymer, which leads to a higher open circuit voltage (V(OC)) and a lower energy loss during electron transfer from the donor to the acceptor. A bulk-heterojunction device fabricated with the cyano-substituted polymer:PC71BM blend has a higher V(OC) (0.89 V), a higher fill factor (FF) (51.4%), and a lower short circuit current (J(SC)) (7.4 mA/cm(2)) than that of the noncyano-substituted polymer:PC71BM blend under AM 1.5G illumination with an intensity of 100 mW cm(-2). Thus, the cyano-substitution of conjugated polymers may be an effective strategy for optimizing the domain size and crystallinity of the polymer:PC71BM blend, and for increasing V(OC) by tuning the HOMO and LUMO energy levels of the conjugated polymer.
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Gefitinib induces cytoplasmic translocation of the CDK inhibitor p27 and its binding to a cleaved intermediate of caspase 8 in non-small cell lung cancer cells.
Cell Oncol (Dordr)
PUBLISHED: 09-03-2014
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The epidermal growth factor receptor (EGFR) represents one of the first rationally selected molecules for targeted therapy in non-small cell lung cancer (NSCLC). Gefitinib is a reversible and highly selective tyrosine kinase inhibitor that competitively blocks the binding of adenosine triphosphate to its binding site in the tyrosine kinase domain of the EGFR. It has been found that treatment with gefitinib induces cell cycle arrest and apoptosis in NSCLC cells harboring activating EGFR mutations. Despite its clinical relevance, however, the mechanism underlying gefitinib-induced apoptosis has remained largely unknown.
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Metabolic engineering of Corynebacterium glutamicum for the production of L-ornithine.
Biotechnol. Bioeng.
PUBLISHED: 08-28-2014
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L-ornithine is a non-essential amino acid for various industrial applications in food industry. In this study, Corynebacterium glutamicum ATCC 13032 was metabolically engineered for the production of L-ornithine. First, the proB and argF genes were deleted to block the competitive branch pathway and to block the conversion of L-ornithine to citrulline, respectively. In addition, the argR gene encoding the regulatory repressor of the L-arginine operon was also deleted. The resulting strain produced 230?mg/L of L-ornithine from glucose in flask culture. This base strain was further engineered by the plasmid-based overexpression of the argCJBD genes from C. glutamicum ATCC 21831, which resulted in the production of 7.19?g/L of L-ornithine. To enrich the NADPH pool, the carbon flux was redirected towards the pentose phosphate pathway by changing the start codons of the pgi and zwf genes and replacing the native promoter of the tkt operon with the strong sod promoter. Fed-batch cultivation of this final strain YW06 (pSY223) allowed production of 51.5?g/L of L-ornithine in 40?h with the overall productivity of 1.29?g/L/h. The results obtained in this study demonstrate the possibility of efficiently producing L-ornithine by metabolically engineered C. glutamicum. Biotechnol. Bioeng. 2014;9999: 1-6. © 2014 Wiley Periodicals, Inc.
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Biopsychological traits of Sasang typology based on Sasang personality questionnaire and body mass index.
BMC Complement Altern Med
PUBLISHED: 08-26-2014
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The purpose of present study was to examine biological and psychological characteristics of people according to the Sasang typology, which is popular in Korea. We evaluated the Sasang Personality Questionnaire (SPQ) as a measure of temperament, and Body Mass Index (BMI) as a measure of the somatic properties of each Sasang type.
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Naphtho[2,1-b:3,4-b']dithiophene-based Bulk Heterojunction Solar Cells: How Molecular Structure Influences Nanoscale Morphology and Photovoltaic Properties.
Chemphyschem
PUBLISHED: 08-21-2014
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Organic bulk heterojunction photovoltaic devices based on a series of three naphtho[2,1-b:3,4-b']dithiophene (NDT) derivatives blended with phenyl-C71 -butyric acid methyl ester were studied. These three derivatives, which have NDT units with various thiophene-chain lengths, were employed as the donor polymers. The influence of their molecular structures on the correlation between their solar-cell performances and their degree of crystallization was assessed. The grazing-incidence angle X-ray diffraction and atomic force microscopy results showed that the three derivatives exhibit three distinct nanoscale morphologies. We correlated these morphologies with the device physics by determining the J-V characteristics and the hole and electron mobilities of the devices. On the basis of our results, we propose new rules for the design of future generations of NDT-based polymers for use in bulk heterojunction solar cells.
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Talking About Health Care: News Framing of Who Is Responsible for Rising Health Care Costs in the United States.
J Health Commun
PUBLISHED: 08-12-2014
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This content analysis examines how the American news media have presented the problem of high and rising health care costs, looking particularly at the question of who is responsible. More specifically, the authors examine how often the media have discussed the 5 major causes of the problem: (a) patients, (b) health care providers, (c) insurance companies, (d) the government, and (d) pharmaceutical companies. Results revealed that patients were most often mentioned as the cause of increasing health care costs. The authors also found that the media's attribution of responsibility to patients has increased over the years. Overall, media coverage of rising health care costs peaked in 1993, 2004, and 2009, suggesting that coverage was influenced by newsworthy events (e.g., the president endorsing legislation or signing a bill into law) that draw the public's attention.
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Toxicologic assessment of Paecilomyces tenuipes in rats: Renal toxicity and mutagenic potential.
Regul. Toxicol. Pharmacol.
PUBLISHED: 07-31-2014
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Paecilomyces tenuipes is entomogenous fungus that is called snow-flake Dongchunghacho in Korea. Although it is widely used in traditional medicines, its safety has not yet been comprehensively investigated. Therefore, the aim of this study was to evaluate the genotoxicity, acute and subchronic toxicity of P. tenuipes. The acute oral LD50 of P. tenuipes extract in rats was estimated to be greater than 2000mg/kg of body weight. In the subchronic study, the oral treatment of rats with 500, 1000 or 2000mg/kg P. tenuipes extract daily for 13weeks did not induce any dose-related changes (body weight, food consumption, clinical observation, urinalysis, hematology, clinical chemistry and organ weight). In contrast, histopathological observation revealed that P. tenuipes extract induced karyomegaly in outer medulla of kidney in all treated rats. Importantly, P. tenuipes extract exerted the mutagenic potential in Ames assay. Since karyomegalic alterations have been known to be associated with carcinogenicity, our finding on the mutagenicity of P. tenuipes extract supports the possibility on the potential involvement of P. tenuipes in carcinogenicity at least partially. In conclusion, the subchronic oral exposure of P. tenuipes may induce kidney abnormality at the concentration higher than 500mg/kg body weight, although further studies using other animal models are needed to identify the toxicity of P. tenuipes.
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Improvement of diagnostic performance regarding retinal nerve fiber layer defect using shifting of the normative database according to vessel position.
Invest. Ophthalmol. Vis. Sci.
PUBLISHED: 07-31-2014
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To evaluate the clinical efficacy of early detection of retinal nerve fiber layer (RNFL) defect in a Korean population using shifting of the normative RNFL thickness database according to vessel position.
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Dramatic Inversion of Charge Polarity in Diketopyrrolopyrrole-Based Organic Field-Effect Transistors via a Simple Nitrile Group Substitution.
Adv. Mater. Weinheim
PUBLISHED: 07-19-2014
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A record-breaking high electron mobility of 7.0 cm(2) V(-1) s(-1) for n-channel polymer OFETs is reported. By the incorporation of only one nitrile group as an electron-withdrawing function in the vinyl linkage of the DPP-based copolymer, a dramatic inversion of majority charge-carriers from holes to electrons is achieved.
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Relationship between visual acuity and retinal structures measured by spectral domain optical coherence tomography in patients with open-angle glaucoma.
Invest. Ophthalmol. Vis. Sci.
PUBLISHED: 07-19-2014
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We assessed the relationship between retinal structures measured by spectral-domain optical coherence tomography (SD-OCT) and visual acuity in open-angle glaucoma (OAG) patients.
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Characterization of biomaterial-free cell sheets cultured from human oral mucosal epithelial cells.
J Tissue Eng Regen Med
PUBLISHED: 07-15-2014
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The purpose of this study was to report the characteristics of biomaterial-free sheets cultured from human oral mucosal epithelial cells without fibrin support, in vitro and after transplantation to limbal-deficient models. Human oral mucosal epithelial cells and limbal epithelial cells were cultured for 2 weeks, and the colony-forming efficiency (CFE) rates were compared. Markers of stem cells (p63), cell proliferation (Ki-67) and epithelial differentiation (cytokeratin; K1, K3, K4, K13) were observed in colonies and in biomaterial-free sheets. Biomaterial-free sheets which had been detached with 1% dispase or biomaterial-free sheets generated by fibrin support were transplanted to 12 limbal-deficient rabbit models. In vitro cell viability, in vivo stability and cytokeratin characteristics of biomaterial-free sheets were compared with those of sheets formed by fibrin-coated culture 1 week after transplantation. Mean CFE rate was significantly higher in human oral mucosal epithelial cells (44.8%) than in human limbal epithelial cells(17.7%). K3 and K4 were well expressed in both colonies and sheets. Biomaterial-free sheets had two to six layers of stratified cells and showed an average of 79.8% viable cells in the sheets after detachment. Cytokeratin expressions of biomaterial-free sheets were comparable to those of sheets cultured by fibrin support, in limbal-deficient models. Both p63 and Ki-67 were well expressed in colonies, isolated sheets and sheets transplanted to limbal-deficient models. Our results suggest that biomaterial-free sheets cultured from human oral mucosal epithelial cells without fibrin support can be an alternative option for cell therapy in use for the treatment of limbal-deficient diseases. Copyright © 2014 John Wiley & Sons, Ltd.
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Instability of 24-hour intraocular pressure fluctuation in healthy young subjects: a prospective, cross-sectional study.
BMC Ophthalmol
PUBLISHED: 06-09-2014
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Elevated intraocular pressure (IOP) is a major risk factor for the development and/or progression of glaucoma, and a large diurnal IOP fluctuation has been identified as an independent risk factor of glaucoma progression. However, most previous studies have not considered the repeatability of 24-hour IOP measurements. The aim of this study was to evaluate the instability of 24-hour IOP fluctuations in healthy young subjects.
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High performance organic nonvolatile flash memory transistors with high-resolution reduced graphene oxide patterns as a floating gate.
ACS Appl Mater Interfaces
PUBLISHED: 05-28-2014
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High-performance organic nonvolatile memory transistors (ONVMTs) are demonstrated, the construction of which is based on novel integration of a highly conductive polymer as a semiconductor layer, hydroxyl-free polymer as a tunneling dielectric layer, and high-resolution reduced graphene oxide (rGO) patterns as a floating gate. Finely patterned rGO, with a line width of 20-120 ?m, was embedded between SiO2 and the polymer dielectric layer, which functions as a nearly isolated charge-trapping center. The resulting ONVMTs demonstrated ideal memory behavior, and the transfer characteristics promptly responded to writing and erasing the gate bias. In particular, the retention time of written/erased states tended to increase as the rGO line width was reduced, implying that the line width is a critical factor in suppressing charge release from rGO. Using a 20-?m-wide rGO pattern, a nonvolatile large memory window (>20 V) was retained for more than 5 × 10(5) s, which is 50 times longer than non-patterned rGO films.
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Reduction of Continuous Theta Burst Stimulation-Induced Motor Plasticity in Healthy Elderly With COMT Val158Met Polymorphism.
Ann Rehabil Med
PUBLISHED: 05-15-2014
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To delineate whether cortical plasticity induced by continuous theta burst stimulation (cTBS) differed according to catechol-O-methyltransferase (COMT) gene polymorphism in healthy older adults.
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The Combination of irreversible EGFR TKIs and SAHA induces apoptosis and autophagy-mediated cell death to overcome acquired resistance in EGFR T790M-mutated lung cancer.
Int. J. Cancer
PUBLISHED: 05-09-2014
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To overcome T790M-mediated acquired resistance of lung cancer cells to epidermal growth factor receptor tyrosine kinase inhibitors (EGFR TKIs), second generation TKIs such as BIBW2992 (afatinib) and third generation TKIs including WZ4002 have been developed. However, clinical data on their efficacy in treating T790M mutant tumors are lacking. Histone deacetylase (HDAC) inhibitors have been reported to arrest cell growth and to lead to differentiation and apoptosis of various cancer cells, both in vitro and in vivo. In the present study, we assessed whether the combination of suberoylanilide hydroxamic acid (SAHA, vorinostat), a potent HDAC inhibitor, and BIBW2992 or WZ4002 could overcome EGFR TKI resistance associated with T790M mutation in lung cancer cells. While treatment with BIBW2992 or WZ4002 alone slightly reduced the viability of PC-9G and H1975 cells, which possess T790M mutation, combining them with SAHA resulted in significantly decreased cell viability through the activation of the apoptotic pathway. This combination also enhanced autophagy occurrence and inhibition of autophagy significantly reduced the apoptosis induced by the combination treatment, showing that autophagy is required for the enhanced apoptosis. Caspase-independent autophagic cell death was also induced by the combination treatment with SAHA and either BIBW2992 or WZ4002. Finally, the combined treatment with SAHA and either BIBW2992 or WZ4002 showed an enhanced anti-tumor effect on xenografts of H1975 cells in vivo. In conclusion, the combination of new generation EGFR TKIs and SAHA may be a new strategy to overcome the acquired resistance to EGFR TKIs in T790M mutant lung cancer. © 2014 Wiley Periodicals, Inc.
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Hierarchical cluster analysis of progression patterns in open-angle glaucoma patients with medical treatment.
Invest. Ophthalmol. Vis. Sci.
PUBLISHED: 05-01-2014
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To classify medically treated open-angle glaucoma (OAG) by the pattern of progression using hierarchical cluster analysis, and to determine OAG progression characteristics by comparing clusters.
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In situ recruitment of human BMSCs using chemokines for articular cartilage regeneration.
Cell Transplant
PUBLISHED: 04-25-2014
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Bone marrow-derived mesenchymal stem cells (BMSCs) are a good cell source for regeneration of cartilage as they can migrate directly to the site of cartilage injury and differentiate into articular chondrocytes. Articular cartilage defects do not heal completely due to the lack of chondrocytes or BMSCs at the site of injury. In this study, the chemotaxis of BMSCs toward chemokines, which may give rise to a complete regeneration of the articular cartilage, was investigated. CCR2, CCR4, CCR6, CXCR1, and CXCR2 were expressed in normal BMSCs and were increased significantly upon treatment with pro-inflammatory cytokines. BMSC migration was increased by MIP-3a and IL-8 more than by MCP-1 or SDF-1?. IL-8 and MIP-3? significantly enhanced the chemotaxis of BMSCs compared with MCP-1, SDF-1?, or the PBS. Human BMSC recruitment to transplanted scaffolds containing either IL-8 or MIP-3? significantly increased in vivo compared with that to scaffolds containing the PBS. Furthermore, IL-8- and MIP-3?-containing scaffolds enhanced tissue regeneration of ostechondral defect site formed in beagle knee articular cartilage. Therefore, this study suggests that IL-8 and MIP-3? are the candidates that induce the regeneration of damaged articular cartilage.
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Interleukin-6 induces the lineage commitment of bone marrow-derived mesenchymal multipotent cells through down-regulation of Sox2 by osteogenic transcription factors.
FASEB J.
PUBLISHED: 04-09-2014
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Bone marrow-derived mesenchymal stromal cells (BM-MSCs) are a heterogeneous population of cells that differ in size and morphology. BM-MSCs become committed to the osteogenic lineage as senescence approaches and lose multipotency. Nevertheless, little is known about the effects of cell-cell interaction between different populations on stemness loss and lineage commitment. The current study aimed to identify mechanisms by which cell-cell interactions between heterogeneous BM-MSCs affect stemness and lineage commitment of multipotent subpopulation. The lineage commitment of primitive multipotent cells was strongly induced in the presence of cytokines secreted by senescent-like cells in a cell culture insert system. Senescent-like cells secreted higher levels of interleukin-6 (IL-6) than primitive multipotent cells in a human cytokine array. IL-6 induced the lineage commitment and stemness loss in multipotent cells by decreasing Sox2 expression. Furthermore, we confirmed that IL-6 decreased the transcriptional activity of Sox2 through up-regulation of Runx2 and Dlx5. We suggest a mechanism by which IL-6 modulates the expression of Sox2, resulting in decreased multipotency and causing primitive multipotent cells to undergo osteogenic lineage commitment. This is the first study to identify mechanisms in which the cell-cell interactions between the different populations play important roles in the stemness loss and lineage commitment of multipotent populations.-Yoon, D. S., Kim, Y. H., Lee, S., Lee, K.-M., Park, K. H., Jang, Y., Lee, J. W. Interleukin-6 induces the lineage commitment of bone marrow-derived mesenchymal multipotent cells through down-regulation of Sox2 by osteogenic transcription factors.
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Adjusted color probability codes for peripapillary retinal nerve fiber layer thickness in healthy Koreans.
BMC Ophthalmol
PUBLISHED: 03-26-2014
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Though a newly developed spectral domain optical coherence tomography (OCT) is at the center of interests for many ophthalmologic researchers and clinicians, its own characteristics are not fully evaluated yet. The main purpose of this study was to establish the adjusted color probability codes for peripapillary retinal nerve fiber layer (RNFL) thickness in healthy Koreans and to compare them with original color codes provided by spectral domain OCT.
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The effect of branched versus linear alkyl side chains on the bulk heterojunction photovoltaic performance of small molecules containing both benzodithiophene and thienopyrroledione.
Phys Chem Chem Phys
PUBLISHED: 03-25-2014
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To evaluate the effect of side chain characteristics on the photovoltaic performance of small molecules containing both benzodithiophene (BDT) and thienopyrroledione (TPD), we designed and synthesized two such molecules, one containing a branched 2-ethylhexyl (2EH) side chain on the BDT unit (BDTEH-TTPD) and the other containing a linear n-octyl (C8) side chain on the BDT unit (BDTO-TTP). The optical and electrochemical properties and crystalline structures of these molecules were examined. Compared to BDTO-TTPD, BDTEH-TTPD, showed stronger light absorption, longer-range ordering and shorter ?-? stacking distances between backbones. As a result, the power conversion efficiency of a bulk heterojunction solar cell based on BDTEH-TTPD (2.40%) was substantially higher than that of the BDTO-TTPD device (1.12%).
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Efficient diketopyrrolopyrrole-based small-molecule bulk-heterojunction solar cells with different electron-donating end-groups.
Chem Asian J
PUBLISHED: 03-12-2014
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A series of small molecules that contained identical ?-spacers (ethyne), a central diketopyrrolopyrrole (DPP) unit, and different aromatic electron-donating end-groups were synthesized and used in organic solar cells (OSCs) to study the effect of electron-donating groups on the device performance. The three compounds, DPP-A-Ph, DPP-A-Na, and DPP-A-An, possessed intense absorption bands that covered a wide range, from 350 to 750?nm, and relatively low HOMO energy levels, from -5.50 to -5.55?eV. DPP-A-An, which contained anthracene end-groups, demonstrated a stronger absorbance and a higher hole mobility than DPP-A-Ph, which contained phenyl groups, and DPP-A-Na, which contained naphthalene units. The power-conversion efficiencies (PCEs) of OSCs based on organic:PC71BM blends (1:1, w/w) with a processed DIO additive were 3.93% for DPP-A-An, 3.02% for DPP-Na, and 2.26% for DPP-A-Ph. These findings suggest that a DPP core that is functionalized with electron-donating capping groups constitutes a promising new class of solution-processable small molecules for OSC applications.
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Chronic ocular hypertensive rat model using microbead injection: comparison of polyurethane, polymethylmethacrylate, silica and polystyene microbeads.
Curr. Eye Res.
PUBLISHED: 03-03-2014
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To establish and assess an ocular hypertensive rat model using intracameral injection with various microbeads of different sizes and materials.
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Oral administration of herbal medicines for the treatment of otitis media with effusion: protocol for a systematic review.
BMJ Open
PUBLISHED: 03-01-2014
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The purpose of this systematic review is to investigate the efficacy of the oral administration of herbal medicines for otitis media with effusion through analysing trial data.
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Novel diketopyrroloppyrrole random copolymers: high charge-carrier mobility from environmentally benign processing.
Adv. Mater. Weinheim
PUBLISHED: 02-24-2014
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The random copolymerization between two different diketopyrrolopyrole-based conducting units represents a suitable synthetic strategy to increase the solubility of polymer semiconductors in a non-chlorinated solvent, without compromising the high charge-carrier mobility. Highly performing thin-film transistors processed from environmentally benign solvents such as tetralin are demonstrated for the first time, resulting in a mobility of greater than 5 cm(2) V(-1) s(-1) .
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Ixodid tick infestation in cattle and wild animals in maswa and iringa, Tanzania.
Korean J. Parasitol.
PUBLISHED: 02-18-2014
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Ticks and tick-borne diseases are important in human and livestock health worldwide. In November 2012, ixodid ticks were collected and identified morphologically from cattle and wild animals in the Maswa district and Iringa urban, Tanzania. Amblyomma gemma, A. lepidum, and A. variegatum were identified from Maswa cattle, and A. variegatum was the predominant species. A. marmoreum, Hyalomma impeltatum, and Rhipicephalus pulchellus were identified from Iringa cattle in addition to the above 3 Amblyomma species, and A. gemma was the most abundant species. Total 4 Amblyomma and 6 Rhipicephalus species were identified from wild animals of the 2 areas. A. lepidum was predominant in Maswa buffaloes, whereas A. gemma was predominant in Iringa buffaloes. Overall, A. variegatum in cattle was predominant in the Maswa district and A. gemma was predominant in Iringa, Tanzania.
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The relationship between gastroesophageal reflux disease and chronic periodontitis.
Gut Liver
PUBLISHED: 02-12-2014
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The most common cause of chronic periodontitis is poor oral hygiene. Gastroesophageal reflux disease (GERD) enhances the proximal migration of gastric contents and may cause poor oral hygiene. We hypothesized that GERD may increase the risk of chronic periodontitis and investigated this potential relationship.
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Analysis of Dosage Mutation in PARK2 among Korean Patients with Early-Onset or Familial Parkinson's Disease.
J Clin Neurol
PUBLISHED: 02-09-2014
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There is some controversy regarding heterozygous mutations of the gene encoding parkin (PARK2) as risk factors for Parkinson's disease (PD), and all previous studies have been performed in non-Asian populations. Dosage mutation of PARK2, rather than a point mutation or small insertion/deletion mutation, was reported to be a risk factor for familial PD; dosage mutation of PARK2 is common in Asian populations.
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Herbal medicines for treating tic disorders: a systematic review of randomised controlled trials.
Chin Med
PUBLISHED: 02-06-2014
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It was reported that 64% of tic disorder patients used complementary and alternative medicine. This review aims to evaluate the efficacy of herbal medicines in treating tic disorders.
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Thieno[3,4-c]pyrrole-4,6-dione-based small molecules for highly efficient solution-processed organic solar cells.
Chem Asian J
PUBLISHED: 01-29-2014
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Two small molecules named BT-TPD and TBDT-TTPD with a thieno[3,4-c]pyrrole-4,6-dione (TPD) unit were designed and synthesized for solution-processed bulk-heterojunction solar cells. Their thermal, electrochemical, optical, charge-transport, and photovoltaic characteristics were investigated. These compounds exhibit strong absorption at 460-560?nm and low highest occupied molecular orbital levels (-5.36?eV). Field-effect hole mobilities of these compounds are 1.7-7.7×10(-3) cm(2) V(-1) s(-1). Small-molecule organic solar cells based on blends of these donor molecules and a acceptor display power conversion efficiencies as high as 4.62% under the illumination of AM 1.5G, 100?mW?cm(-2).
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Ferroelectric coupling effect on the energy-band structure of hybrid heterojunctions with self-organized P(VDF-TrFE) nanomatrices.
Adv. Mater. Weinheim
PUBLISHED: 01-25-2014
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Ferroelectric coupling effects on the energy-band structure of hybrid heterojunctions are investigated using hybrid photovoltaic devices with poly(3-hexylthiophene-2,5-diyl) (P3HT)/ZnO and poly(vinylidene fluoride-trifluoroethylene) (P(VDF-TrFE)). The self-organized P(VDF-TrFE):P3HT photoactive layer forms a novel architecture consisting of P3HT domains in a P(VDF-TrFE) matrix. The energy-band structure at the interface of the p-n heterojunction is tuned by artificial control of the ferroelectric polarization of the P(VDF-TrFE) material, consequently modulating the photovoltaic performance of the hybrid photovoltaic devices.
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Variations of retinal nerve fiber layer thickness and ganglion cell-inner plexiform layer thickness according to the torsion direction of optic disc.
Invest. Ophthalmol. Vis. Sci.
PUBLISHED: 01-25-2014
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To examine the relationship between the optic disc torsion and peripapillary retinal nerve fiber layer (RNFL) thickness through a comparison with the macular ganglion cell inner plexiform layer complex (GCIPL) thickness measured by Cirrus optical coherence tomography (OCT).
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The distribution of intraocular pressure and associated systemic factors in a Korean population: The Korea National Health and Nutrition Examination Survey.
Acta Ophthalmol
PUBLISHED: 01-22-2014
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To investigate the distribution of intraocular pressure (IOP) and its associated factors in a large Korean population based on the data from the nationwide cross-sectional survey.
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Diurnal intraocular pressure with bimatoprost/timolol fixed combination versus latanoprost/timolol fixed combination in healthy subjects.
Korean J Ophthalmol
PUBLISHED: 01-21-2014
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To evaluate the effects of a bimatoprost/timolol fixed combination (BTFC) and a latanoprost/timolol fixed combination (LTFC) on diurnal intraocular pressure (IOP) and anterior ocular parameters in healthy subjects.
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Heritabilities of intraocular pressure in the population of Korea: the Korean National Health and Nutrition Examination Survey 2008-2009.
JAMA Ophthalmol
PUBLISHED: 01-18-2014
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Intraocular pressure (IOP) is the principal modifiable risk factor for the progression of primary open-angle glaucoma. Studies that have measured the IOP directly in large numbers of matched parent-offspring pairs are limited in Asian populations.
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Innovative strength training-induced neuroplasticity and increased muscle size and strength in children with spastic cerebral palsy: an experimenter-blind case study--three-month follow-up.
NeuroRehabilitation
PUBLISHED: 01-15-2014
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In children with cerebral palsy (CP), the never-learned-to-use (NLTU) effect and underutilization suppress the normal development of cortical plasticity in the paretic limb, which further inhibits its functional use and increases associated muscle weakness.
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Increase in intraocular pressure is less with propofol than with sevoflurane during laparoscopic surgery in the steep Trendelenburg position.
Can J Anaesth
PUBLISHED: 01-14-2014
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Intraocular pressure is increased during laparoscopic surgeries performed in a steep Trendelenburg position. This study compared the effects of propofol with those of sevoflurane on intraocular pressure in patients undergoing robot-assisted laparoscopic radical prostatectomy in a 30° Trendelenburg position.
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Sho-saiko-to, a traditional herbal medicine, regulates gene expression and biological function by way of microRNAs in primary mouse hepatocytes.
BMC Complement Altern Med
PUBLISHED: 01-11-2014
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Sho-saiko-to (SST) (also known as so-shi-ho-tang or xiao-chai-hu-tang) has been widely prescribed for chronic liver diseases in traditional Oriental medicine. Despite the substantial amount of clinical evidence for SST, its molecular mechanism has not been clearly identified at a genome-wide level.
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Expansion of the clinicopathological and mutational spectrum of Perry syndrome.
Parkinsonism Relat. Disord.
PUBLISHED: 01-09-2014
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Perry syndrome (PS) caused by DCTN1 gene mutation is clinically characterized by autosomal dominant parkinsonism, depression, severe weight loss, and hypoventilation. Previous pathological studies have reported relative sparing of the cerebral cortex in this syndrome. Here, we characterize novel clinical and neuroimaging features in 3 patients with PS.
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Determination of Polyphenol Components of Korean Prostrate Spurge (Euphorbia supina) by Using Liquid Chromatography-Tandem Mass Spectrometry: Overall Contribution to Antioxidant Activity.
J Anal Methods Chem
PUBLISHED: 01-02-2014
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The Korean prostrate spurge Euphorbia supina is a weed that has been used in folk medicine in Korea against a variety of diseases. Nine polyphenols were characterized for this plant by using high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) and the results were compared with the literature data. The individual components were validated using the calibration curves of structurally related external standards and quantified for the first time by using the validated method. Correlation coefficients (r (2)) were >0.9907. The limit of detection and limit of quantification of the method were >0.028?mg/L and 0.094?mg/L, respectively. Recoveries measured at 50?mg/L and 100?mg/L were 76.1-102.8% and 85.2-98.6%, respectively. The total amount of the identified polyphenols was 3352.9?±?2.8?mg/kg fresh plant. Quercetin and kaempferol derivatives formed 84.8% of the total polyphenols. The antioxidant activities of the flavonoids were evaluated in terms of 1,1-diphenyl-2-picrylhydrazyl and 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) radical cation-scavenging activity, and the reducing power showed a dose-dependent increase. Cell viability was effectively suppressed at polyphenol mixture concentrations >250?mg/L.
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Glaucoma diagnostic value of the total macular thickness and ganglion cell-inner plexiform layer thickness according to optic disc area.
Br J Ophthalmol
PUBLISHED: 01-02-2014
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This study examined the diagnostic value of the perimacular ganglion cell-inner plexiform layer (GCIPL) thickness in the detection of glaucoma according to the optic disc area.
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Neuroprotective and neurite outgrowth effects of maltol on retinal ganglion cells under oxidative stress.
Mol. Vis.
PUBLISHED: 01-01-2014
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To evaluate the neuroprotective and neurite outgrowth effects of maltol, a natural aroma compound, on retinal ganglion cells (RGCs) under oxidative stress in vitro.
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Systemic hypertension as a risk factor for open-angle glaucoma: a meta-analysis of population-based studies.
PLoS ONE
PUBLISHED: 01-01-2014
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Systemic hypertension is thought to increase the risk for developing open-angle glaucoma (OAG) through several mechanisms. However, previous epidemiological studies have shown conflicting results regarding this potential association. We systematically evaluated this issue by conducting a meta-analysis of population-based studies.
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Discrimination of Lycium chinense and Lycium barbarum by taste pattern and betaine analysis.
Int J Clin Exp Med
PUBLISHED: 01-01-2014
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Lycii Fructus was used as natural products with therapeutic properties for a long time. Betaine is a natural amino acid and one of the major constituents of Lycii Fructus. It is reported that this fruit plays a role in reducing blood levels of homocysteine, a toxic byproduct of the amino acid metabolism. This study was used to establish infra based on oriental medicine through the analysis of correlation of taste, contents of betaine, %Brix and physico-chemical properties of Lycii Fructus. To investigate betaine, quantitative analysis was performed using HPLC separation system. In addition, %Brix and saccharide were estimated. Taste pattern analysis was measured using the taste sensing system, SA402B equipped with six taste sensors including newly developed sweetness sensor. Betaine quantitative analysis showed that L. barbarum 0.64 ± 0.15% (n = 6) was significantly higher than L. chinense 0.55 ± 0.1% (n = 12). And %Brix and saccharide composition of Lycii Fructus analysis showed that L. barbarum was significantly higher than L. chinense. The results of taste pattern analysis between L. barbarum and L. chinense showed a significant difference in almost every taste. In contrast, sweetness of L. barbarum was higher than L. chinense. When clustering with sweetness and bitterness, the two species are distinctly separated. In conclusion, these taste patterns, %Brix, betaine, and saccharide composition analysis could be applied to the establishment of herbal medicine marker for identification of different species in various regions.
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Achieving high sensitivity in hybrid photodetectors based on an organic single crystal and an inorganic nanocrystal array.
Nanotechnology
PUBLISHED: 12-17-2013
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We demonstrate an approach to enhance the photosensitivity of an organic single-crystal photodetector by combining it with a nanocrystal (NC) array. A systematic study of the dependence of the photodetector performance on illumination wavelength and light power together with the charge carrier mobility reveals that charge separation at the single-crystal/CdSe NC interface and subsequent electron trapping in the CdSe NCs generates effective photoconductive gain by hole circulation through the high-mobility single crystal. As a result, the responsivity and specific detectivity reached values up to 50 A W(-1) and 2 × 10(9) cm Hz(1/2) W(-1), respectively.
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Herbal medicines for the treatment of acute otitis media: protocol for a systematic review.
BMJ Open
PUBLISHED: 12-03-2013
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The aim of this systematic review is to analyse the trial data on the efficacy of herbal medicines for acute otitis media.
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Record high hole mobility in polymer semiconductors via side-chain engineering.
J. Am. Chem. Soc.
PUBLISHED: 09-25-2013
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Charge carrier mobility is still the most challenging issue that should be overcome to realize everyday organic electronics in the near future. In this Communication, we show that introducing smart side-chain engineering to polymer semiconductors can facilitate intermolecular electronic communication. Two new polymers, P-29-DPPDBTE and P-29-DPPDTSE, which consist of a highly conductive diketopyrrolopyrrole backbone and an extended branching-position-adjusted side chain, showed unprecedented record high hole mobility of 12 cm(2)/(V·s). From photophysical and structural studies, we found that moving the branching position of the side chain away from the backbone of these polymers resulted in increased intermolecular interactions with extremely short ?-? stacking distances, without compromising solubility of the polymers. As a result, high hole mobility could be achieved even in devices fabricated using the polymers at room temperature.
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Body fat assessment method using CT images with separation mask algorithm.
J Digit Imaging
PUBLISHED: 09-21-2013
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In recent years, the number of obese population in Korea has been growing up along with the economic development, environmental factors, and the change in life style. Considering the growth of obese population and the adverse effect of obesity on health, it is getting more important to prevent and diagnose the obesity with the quantitative measurement of body fat that has become an important indicator for obesity. In this study, we proposed a procedure for the automated fat assessment from computed tomography (CT) data using image processing technique. The proposed method was applied to a single-CT image as well as CT-volume data, and results were correlated to those of dual-energy X-ray absorptiometry (DEXA) that is known as the reliable method for evaluating body fat. Using single-CT images, correlation coefficients between DEXA and the automated assessment and DEXA and the manual assessment were 0.038 and 0.058, respectively (P?>?0.05). Hence, there was no significant correlation between three methods using the proposed method with single-CT images. On the other hand, in case of CT-volume data, the above correlation coefficients were increased to 0.826, 0.812, and 0.805, respectively (P?
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Deep-blue phosphorescence from perfluoro carbonyl-substituted iridium complexes.
J. Am. Chem. Soc.
PUBLISHED: 09-16-2013
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The new deep-blue iridium(III) complexes, (TF)2Ir(pic), (TF)2Ir(fptz), (HF)2Ir(pic), and (HF)2Ir(fptz), consisting of 2,4?-difluororphenyl-3-methylpyridine with trifluoromethyl carbonyl or heptafluoropropyl carbonyl at the 3 position as the main ligand and a picolinate or a trifluoromethylated-triazole as the ancillary ligand, were synthesized and characterized for applications in organic light-emitting diodes (OLEDs). Density function theory (DFT) calculations showed that these iridium complexes had a wide band gap, owing to the introduction of the strong electron withdrawing perfluoro carbonyl group. Time-dependent DFT (TD-DFT) calculations suggested that their lowest triplet excited state was dominated by a HOMO ? LUMO transition and that the contribution of the metal-to-ligand charge transfer (MLCT) was higher than 34% for all four complexes, indicating that strong spin-orbit coupling exists in the complexes. The 10 wt % (TF)2Ir(pic) doped 9-(3-(9H-carbazole-9-yl)phenyl)-3-(dibromophenylphosphoryl)-9H-carbazole (mCPPO1) film exhibited the highest photoluminescence quantum yield of 74 ± 3% among the films based on the four complexes. Phosphorescent OLEDs based on (TF)2Ir(pic) and (TF)2Ir(fptz) exhibited maximum external quantum efficiencies of 17.1% and 8.4% and Commission Internationale de lEclairage (CIE) coordinates of (0.141, 0.158) and (0.147, 0.116), respectively. These CIE coordinates represent some of the deepest blue emissions ever achieved from phosphorescent OLEDs with considerably high EQEs.
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Determination of chokeberry (Aronia melanocarpa) polyphenol components using liquid chromatography-tandem mass spectrometry: Overall contribution to antioxidant activity.
Food Chem
PUBLISHED: 09-03-2013
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The type and content of plant polyphenols can be influenced by maturity. Korean chokeberry (Aronia melanocarpa) leaves of three different maturities (young, mature, and aged) were extracted with 70% aqueous methanol. The polyphenols in the leaves were analysed for the first time using high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) and comparison with reported data. Among the 12 characterised components, five flavonoids, 3, 4, and 10-12, and a dicaffeoylquinic acid derivative, 6, were characterised for the first time in chokeberry leaves. Each polyphenol component was validated and quantified using a representative polyphenol standard of the same group. The antioxidant activity of the three different mature leaf extracts was determined. The antioxidant activity was highest for young leaves, followed by mature and aged leaves. The results suggest that younger chokeberry leaves may be more favourable for processing a higher quality functional tea due to their higher polyphenol content.
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Charge transport characteristics of a high-mobility diketopyrrolopyrrole-based polymer.
Phys Chem Chem Phys
PUBLISHED: 08-03-2013
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In this study, we attempt to unveil the charge-transport abnormality of the widely studied diketopyrrolopyrrole (DPP)-based polymers with exceptionally high charge carrier mobility [>5 cm(2) V(-1) s(-1)]. Based on the electric field and temperature dependence of the charge-transport characteristics of the field effect transistor (FET) geometry of one of the highly conductive DPP derivatives, namely, (poly[2,5-bis(7-decylnonadecyl)pyrrolo[3,4-c]pyrrole-1,4(2H,5H)-dione-(E)-(1,2-bis(5-(thiophen-2-yl)selenophen-2-yl)ethene) (PDPPDTSE), we show that the high gate-source bias drew the carriers closer to the interface of the semiconductor/dielectric layers where the density of state (DOS) of the charge carrier is significantly broader than the bulk. We argue that the intrinsically narrow DOS in the PDPPDTSE bulk resulted in significantly different charge-transport behavior between the semiconductor bulk and the semiconductor/dielectric interface, which was not visible in the other low-mobility organic semiconductors that contain intrinsically high density of trap states in their bulk. To avoid these charge transport abnormalities, we try to operate the FETs under low gate bias without compromising the accumulated charge carrier density. By carefully employing a thin metal oxide covered with a self-assembled monolayer (SAM) as a dielectric layer, we can demonstrate low-voltage PDPPDTSE FETs with near-ideal performance both in terms of hysteresis-free operation and operating reliability while maintaining a high charge carrier mobility of ~2.8 cm(2) V(-1) s(-1).
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Highly Stretchable Piezoelectric-Pyroelectric Hybrid Nanogenerator.
Adv. Mater. Weinheim
PUBLISHED: 07-31-2013
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A highly stretchable hybrid nanogenerator has been developed using a micro-patterned piezoelectric polymer P(VDF-TrFE), PDMS-CNT composite, and graphene nanosheets. Mechanical and thermal energies were simultaneously harvested from single cell of device. The hybrid nanogenerator exhibited high robustness behavior even after 30% stretching and generated very stable piezoelectric and pyroelectric power outputs due to micro-pattern designing.
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Comparison of the optic nerve imaging by time-domain optical coherence tomography and Fourier-domain optical coherence tomography in distinguishing normal eyes from those with glaucoma.
J. Glaucoma
PUBLISHED: 07-10-2013
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To assess agreement in cup-to-disc ratio (CDR) estimation between stereoscopic optic disc photography, time-domain optical coherence tomography (OCT), and Fourier-domain OCT, and to compare the discriminating ability of optic nerve head (ONH) parameters by time-domain OCT and Fourier-domain OCT for glaucoma detection.
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Comparative analysis of mesenchymal stem cell surface marker expression for human dental mesenchymal stem cells.
Regen Med
PUBLISHED: 07-06-2013
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Human dental mesenchymal stem cells (hDMSCs) have been isolated from extracted human teeth and proven to have different proliferation and differentiation abilities among the subtypes. Despite increasing interest in the clinical use of hDMSCs, a well-defined specific marker has been absent for these stem cells. In this study, a comparative analysis with known mesenchymal stem cell surface markers such as STRO-1, CD90, CD146, CD34 and TfR (CD71) was performed.
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Synthesis and transistor properties of asymmetric oligothiophenes: relationship between molecular structure and device performance.
Chemistry
PUBLISHED: 07-04-2013
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A series of three thiophene-naphthalene-based asymmetric oligomers-5-decyl-2,2:5,2:5,2-quaterthiophene (DtT), 5-decyl-5-(naphthalen-2-yl)-2,2:5,2-terthiophene (D3TN), and 5-(4-decylphenyl)-5-(naphthalen-2-yl)-2,2-bithiophene (DP2TN)-was synthesized by Suzuki cross-coupling reactions. The long alkyl side chains improved both the solubility of the oligomers in solvents and their tendency to self-assemble. UV/Vis absorption measurements suggested that DtT, D3TN, and DP2TN form H-type aggregates with a face-to-face packing structure. In addition, the three oligomers were found to adopt vertically aligned crystalline structures in films deposited on substrates, as revealed by grazing-incidence wide-angle X-ray scattering. These oligomers were used as the active layers of p-type organic field-effect transistors, and the resulting devices showed field-effect mobilities of 3.3×10(-3) ?cm(2) ?V(-1) ?s(-1) for DtT, 1.6×10(-2) ?cm(2) ?V(-1) ?s(-1) for D3TN, and 3.7×10(-2) ?cm(2) ?V(-1) ?s(-1) for DP2TN. The differences in transistor performances were attributed to the degree of ? overlap and the morphological differences determined by the molecular structures.
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In developing our video relationships, we compare around 5 million PubMed articles to our library of over 4,500 methods videos. In some cases the language used in the PubMed abstracts makes matching that content to a JoVE video difficult. In other cases, there happens not to be any content in our video library that is relevant to the topic of a given abstract. In these cases, our algorithms are trying their best to display videos with relevant content, which can sometimes result in matched videos with only a slight relation.