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Find video protocols related to scientific articles indexed in Pubmed.
Identification of candidate biomarkers for the early detection of nasopharyngeal carcinoma by quantitative proteomic analysis.
J Proteomics
PUBLISHED: 05-12-2014
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Nasopharyngeal carcinoma (NPC) is a major head and neck cancer with high occurrence in Southeast Asia and southern China. To identify novel biomarkers for the early detection of NPC patients, 2D-DIGE combined with MALDI-TOF-MS analysis was performed to identify differentially expressed proteins in the carcinogenesis and progression of NPC using LCM-purified normal nasopharyngeal epithelial tissues and various stages of NPC biopsies. As a result, 26 differentially expressed proteins were identified, of which two proteins with sharp expressional changes in the carcinogenic process, ENO1 and CYPA, were validated by western blot analysis and identified as critical seed proteins in the functional network. Immunohistochemistry assay was further performed to detect the expression of the two proteins with a tissue microarray that included various stages of NPC tissues. The ability of these proteins to detect NPC early was evaluated via a receiver operating characteristic analysis. The results indicated that the combination of the two proteins could perfectly discriminate NNET and AH from stage I of NPC with high sensitivity and specificity, which is more effective than using either of the two proteins individually. In summary, the combination of ENO1 and CYPA can serve as potential molecular markers for the early detection of NPC.
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On plant bugs of conifers in Xinjiang (Western China)  (Hemiptera: Heteroptera: Miridae).
Zootaxa
PUBLISHED: 03-05-2014
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In the northern and central part of Xinjiang (Western China) are distributed 14 species of coniferous plant bugs of these, 10 species are recorded for the first time for China. In the Mongolian Altai are 9 species: 6 widely distributed in the Palearctic, Deraeocoris annulipes (Herrich-Schaeffer, 1842), Dichrooscytus intermedius Reuter, 1885, Pinalitus rubricatus (Fallén, 1807), Atractotomus morio J. Sahlberg, 1883, Plagiognathus vitellinus (Scholtz, 1847), Phoenicocoris obscurellus (Fallén, 1829), and 3 Siberian Psallus (Pityopsallus) laricinus Vinokurov, 1998, P. (P.) laticeps Reuter, 1878, P. (P.) sachaensis Vinokurov, 1998. In Chinese Tian Shan and Jungar Alatau occur 5 mountain-Central Asian species: Dichrooscytus consorbinus Horváth, 1904, D. josifovi Kerzhner, 1997, D. kerzhneri Josifov, 1974 and D. pseudosabinae Reuter, 1896, and Compsidolon schrenkianum Konstantinov, Vinokurov, 2011. A key for 5 species of the subgenus Pithyopsallus Wagn. is given.
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miR-181 subunits enhance the chemosensitivity of temozolomide by Rap1B-mediated cytoskeleton remodeling in glioblastoma cells.
Med. Oncol.
PUBLISHED: 02-11-2014
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Glioblastoma multiforme (GBM) is the most malignant and frequent brain tumor, with an aggressive growth pattern and poor prognosis despite best treatment modalities. Although chemotherapy with temozolomide (TMZ) may restrain tumor growth for some months, TMZ resistance is also common and accounts for many treatment failures. Research into microRNA's role in GBM has shown that microRNAs play a key regulatory role in the GBM, making it a potential therapeutic target. In this study, we demonstrated that the lower expression of miR-181a/b/c/d subunits contributes to astrocytoma tumorigenesis, and their overexpression could inhibit the invasive proliferation of glioblastoma cells by targeting Rap1B-mediated cytoskeleton remodeling and related molecular (Cdc42, RhoA and N-cadherin) changes, suggesting that miR-181 was a critical regulator and might be an important target for glioblastoma treatment. TMZ as a standard chemotherapeutic agent for GBM inhibited the Rap1B expression and actin cytoskeleton remodeling to exert its cell killing by upregulating miR-181a/b/c/d subunits; conversely, each miR-181a/b/c/d subunit enhanced the chemosensitivity of TMZ in glioblastoma cells.
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Lactoferrin suppresses the Epstein-Barr virus-induced inflammatory response by interfering with pattern recognition of TLR2 and TLR9.
Lab. Invest.
PUBLISHED: 02-03-2014
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Epstein-Barr virus (EBV) infection contributes to tumorigenesis of various human malignancies including nasopharyngeal carcinoma (NPC). EBV triggers innate immune and inflammatory responses partly through Toll-like receptor (TLR) signaling. Lactoferrin (LF), with its anti-inflammatory properties, is an important component of the innate immune system. We previously reported that LF protects human B lymphocytes from EBV infection by its ability to bind to the EBV receptor CD21, but whether LF can suppress EBV-induced inflammation is unclear. Here, we report that LF reduced synthesis of IL-8 and monocyte chemoattractant protein-1 (MCP-1) induced by EBV in macrophages via its suppression of NF-?B activity. LF interacted with TLR2 and interfered with EBV-triggered TLR2-NF-?B activation. LF inhibited the ability of TLR9 to recognize dsDNA by binding to its co-receptor CD14, which blocked the interaction between CD14 and TLR9. EBV-induced inflammation was thus aggravated in the presence of CD14. In addition, LF expression levels were significantly downregulated in NPC specimens, and correlated inversely with IL-8 and MCP-1 expression. These findings suggest that LF may suppress the EBV-induced inflammatory response through interfering with the activation of TLR2 and TLR9.
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Spatial distribution, temporal variation, and sources of heavy metal pollution in groundwater of a century-old nonferrous metal mining and smelting area in China.
Environ Monit Assess
PUBLISHED: 01-14-2014
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This study first presents the spatial distribution, temporal variation, and sources of heavy metal pollution in groundwater of a nonferrous metal mine area in China. Unconfined groundwater was polluted by Pb, Zn, As, and Cu, in order, while confined karst water in the mines showed pollution in the following sequence: Zn, Cd, Cu, Pb, and As. Pollution by Pb was widespread, while Zn, As, Cu, and Cd were found to be high in the north-central industrial region and to decrease gradually with distance from smelters and tailings. Vertically, more Pb, Zn, Cu, and Cd have accumulated in shallow Quaternary groundwater, while more As have migrated into the deeper fracture groundwater in the local discharge area. Zn, Cd, and Cu concentrations in groundwater along the riverside diminished owing to reduced wastewater drainage since 1977, while samples in the confluence area were found to have increasing contents of Pb, Zn, As, Cu, and Cd since industrialization began in the 1990s. Sources of heavy metals in groundwater were of anthropogenic origin except for Cr. Pb originated primarily from airborne volatile particulates, wastewater, and waste residues and deposited continuously, while Zn, Cd, and Cu were derived from the wastewater of smelters and leakage of tailings, which corresponded to the related soil and surface residue researches. Elevated As values around factories might be the result of chemical reactions. Flow patterns in different hydrogeological units and adsorption capability of from Quaternary sediments restricted their cross-border diffusion.
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Prohibitin is an important biomarker for nasopharyngeal carcinoma progression and prognosis.
Eur. J. Cancer Prev.
PUBLISHED: 09-04-2013
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Prohibitin-1 (PHB, also known as PHB1) is a pleiotropic protein in cells. PHB is a cell-surface receptor and is involved in the regulation of proliferation, apoptosis, transcription, and mitochondrial protein folding. PHB is upregulated in 5-8F cells, which overexpress LPLUNC1 (long palate, lung, nasal epithelium clone 1, a candidate tumour suppressor gene), and was identified using two-dimensional fluorescence difference gel electrophoresis and matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF/TOF-MS/MS). Thus, we examined PHB mRNA levels using 24 nasopharyngeal carcinoma (NPC) and eight normal nasopharyngeal epithelium (NPE) tissues. Protein levels were detected using immunohistochemistry with a tissue microarray consisting of 323 NPC and NPE tissues. A Kaplan-Meier analysis was carried out, and the log-rank test was used to determine the statistical significance of the results using SPSS 15.0 software. PHB mRNA and protein expression levels were significantly downregulated in NPC tissue specimens compared with the NPE samples (P<0.01). In addition, decreased PHB expression correlates significantly with a poor prognosis, whereas decreased PHB protein expression is closely associated with advanced clinical stage and metastasis in NPC lesions. Therefore, we favour the hypothesis that the expression level of PHB could be used as a potential prognostic biomarker for NPC.
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Expression of miR-126 suppresses migration and invasion of colon cancer cells by targeting CXCR4.
Mol. Cell. Biochem.
PUBLISHED: 02-02-2013
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A previous study demonstrated that miR-126 expression was significantly downregulated in highly metastatic colon cancer cells. This study was to investigate the biological function of miR-126 and its regulation of target genes in colon cancer cells. Quantitative PCR was used to detect miR-126 expression in colon cancer SW480 and SW620 cells. MTT assay was to measure the changed cell viability after miR-126 mimics transfection. Wound healing and Transwell migration and invasion assays measured capacity of tumor cell migration and invasion of SW480 and SW620 cells after miR-126 transfection. Luciferase reporter assay and Western blot were used to assess both transcriptional and expression levels of one of the miR-126 target genes (i.e., CXCR4). Levels of miR-126 expression were lower in colon cancer SW480 and SW620 cells than in the adjacent normal epithelial tissues (P < 0.05). Transfection of miR-126 mimics significantly reduced colon cancer cell viability compared to NC cells (P < 0.05). The wound healing and Transwell migration and invasion assays showed that miR-126 mimics inhibited SW480 and SW620 cell migration and invasion capacity. Bioinformatics predicted that CXCR4 is one of the miR-126 target genes. Indeed, luciferase reporter assay and Western blot confirmed that CXCR4 is a miR-126 target gene. Expression of miR-126 inhibited colon cancer cell viability and reduced tumor cell migration and invasion capacity by its negative regulation of CXCR4 expression.
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Epstein-Barr virus downregulates microRNA 203 through the oncoprotein latent membrane protein 1: a contribution to increased tumor incidence in epithelial cells.
J. Virol.
PUBLISHED: 12-28-2011
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The Epstein-Barr virus (EBV) is highly associated with nasopharyngeal carcinoma (NPC), and it regulates some microRNAs (miRNAs) that are involved in the development of cancer. The role of EBV in the deregulation of cellular miRNAs and how this affects the progression of NPC remain to be investigated. An analysis of the miRNA profile in an EBV-infected cell line revealed that miRNA 203 (miR-203) was downregulated. miR-203 is expressed specifically in epithelial cells. This downregulation of miR-203 was further verified and functionally analyzed. miR-203 was downregulated substantially in epithelial cells and NPC tissues that were latently infected with EBV. Downregulation of miR-203 also occurred during the early stage of EBV infection. Furthermore, the viral oncoprotein, latent membrane protein 1 (LMP1), was responsible for downregulation of miR-203. Removal of the latent EBV genome or suppression of LMP1 resulted in restoration of miR-203 expression. EBV-LMP1 mediated the downregulation of miR-203 at the primary transcript level. E2F3 and CCNG1 were identified as target genes of miR-203. Ectopic expression of miR-203 inhibited EBV-induced S-phase entry and transformation in vivo. Overexpression of the targets overcame the effects of miR-203 mimics on the cell cycle, and the expression of target genes in tumor models was inhibited by miR-203. Inhibitors of Jun N-terminal protein kinase (JNK) and NF-?B blocked miR-203 downregulation. These results imply that EBV promotes malignancy by downregulating cellular miR-203, which contributes to the etiology of NPC.
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Tumor-conditioned mesenchymal stem cells display hematopoietic differentiation and diminished influx of Ca2+.
Stem Cells Dev.
PUBLISHED: 11-11-2011
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Mesenchymal stem cells (MSCs) that are present in many adult tissues can generate new cells either continuously or in response to injury/cancer. An increasing number of studies demonstrated that MSCs have the ability to differentiate into cells of mesodermal origin and transdifferentiate into cells such as hepatocytes, neural cells. There has been growing interest in the application of MSCs to cancer therapy. The relationship between MSCs and cancer cells remains highly controversial. In this study, we analyzed the interaction of bone marrow-derived MSCs and cancer cells by cell-cell contact and transwell culture system. The flow cytometry and real-time polymerase chain reaction showed that after coculture of MSCs and cancer cells, MSCs displayed the hematopoietic cell markers such as CD34, CD45, and CD11b. The CD68, MRCI, and CSF1R were dramatically upregulated after coculture. The cytokine array showed that MSCs after coculture secreted monokines and chemokines much more than that of intact MSCs. The MSCs under tumor conditions were responsive to stimulation with lipopolysaccharide by cytokines release. The tumor-conditioned MSCs showed phagocytic ability and enhanced release of nitric oxide, which are the characteristics of macrophages. Calcium ion is an important intracellular messenger responsible for differentiation and gene expression regulations. The influx of Ca(2+) into MSCs was obviously reduced after coculture. The blocking of calcium channel with verapamil obviously increased the expression of CD34, CD45, and CD11b, thus indicating that the diminished calcium ion influx is coupled with the hematopoietic differentiation of MSCs under tumor conditions. Taken together, in a cancer environment, MSCs could effectively differentiate into immune hematopoietic cells, precisely macrophages. Diminished transient influx of Ca(2+) may mediate the hematopoietic differentiation of MSCs.
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miR-149 promotes epithelial-mesenchymal transition and invasion in nasopharyngeal carcinoma cells.
Zhong Nan Da Xue Xue Bao Yi Xue Ban
PUBLISHED: 08-30-2011
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To investigate the function and mechanism of miR-149 in nasopharyngeal carcinoma (NPC).
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Cis-regulatory functions of overlapping HIF-1alpha/E-box/AP-1-like sequences of CD164.
BMC Mol. Biol.
PUBLISHED: 08-09-2011
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CD164 (also known as MGC-24v or endolyn) is a sialomucin which has been suggested to participate in regulating the proliferation, cell adhesion and differentiation of hematopoietic stem and progenitor cells. CD164 is also involved in the development of cancer. The functions of cis-regulatory elements of CD164 remain relatively unknown.
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The microRNA-processing enzymes: Drosha and Dicer can predict prognosis of nasopharyngeal carcinoma.
J. Cancer Res. Clin. Oncol.
PUBLISHED: 07-14-2011
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Dysregulation of microRNA (miRNA) metabolism has been observed in a variety of human cancers, but the expression patterns of the enzymes responsible for generating miRNAs remain largely unexplored. In this study, we investigated the expression profiles of the two most important enzymes of the miRNA machinery, Drosha and Dicer, which were closely correlated with nasopharyngeal carcinoma (NPC) and patient survival.
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Emergy evaluation of the natural value of water resources in Chinese rivers.
Environ Manage
PUBLISHED: 05-25-2009
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Emergy theory and method were used to evaluate the economy of China and the contributions of water resources in Chinese rivers to the real wealth of the Chinese economy. The water cycle and energy conversion were reviewed, and an emergy method for evaluating the natural value of water resources in a river watershed was developed. The indices for China calculated from the emergy evaluation were close to those of developing countries. Despite a small surplus in its balance of payments, China had a net emergy loss from its trade in 2002. The efficiency of Chinese natural resource use was still not high and did not match its economic growth rate. Furthermore, the Chinese economy placed a stress on its ecological environment and natural resources. Several indices of Chinese rivers from the emergy evaluation were close to those of average global river water. The main average indices of Chinese rivers were transformity (4.17 x 10(4) sej/J), emergy per volume (2.05 x 10(11) sej/m(3)), and emdollar per volume (0.06 $/m(3)). The total value of all the rivers water made up 13.0% of the GDP of China in 2002, and that of water consumption accounted for 2.1%. The value of the water resources in the Haiheluanhe River (11.39 x 10(4) sej/J) was the highest, followed by the Yellow River (10.27 x 10(4) sej/J), while the rivers in Southwest China had the lowest values (2.92 x 10(4) sej/J).
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Multidimensional GC-fourier transform ion cyclotron resonance MS analyses: utilizing gas-phase basicities to characterize multicomponent gasoline samples.
J Chromatogr Sci
PUBLISHED: 01-24-2009
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Hydrocarbon isomers, present in crude petroleum, may yield similar gas chromatography (GC) retention times and indistinguishable mass spectral patterns. Hence, conventional GC-mass spectrometry (MS) may not provide sufficient data for identification of hydrocarbon isomers. Real-time proton affinity or gas-phase basicity "bracketing" provides an additional dimension to GC-MS analyses. Our GC-fourier transform (FT)-ion cyclotron resonance (ICR)-MS yielded an average mass measurement error of less than 3 ppm for components of a retail gasoline sample. The combined use of concurrent thermo-chemical measurements with GC-FT-ICR-MS data analysis allowed differentiation of various isomers such as C(8)H(10) species.
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miR-18a promotes malignant progression by impairing microRNA biogenesis in nasopharyngeal carcinoma.
Carcinogenesis
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Dysregulation of microRNA (miRNA) biogenesis is implicated in cancer development and progression. Dicer and Drosha are established regulators of miRNA biogenesis. In this study, we used a miRNA array to evaluate the miRNA expression profiles in nasopharyngeal carcinoma (NPC) samples. The significance analysis of microarrays showed a global downregulation of miRNA expression in NPC samples compared with normal nasopharyngeal epithelial tissues. Notably, miR-18a, a member of the oncogenic miR-17-92 cluster, was upregulated in the NPC samples and cell lines. Clinical parameter studies showed that higher levels of miR-18a correlated with NPC advanced stage, lymph node metastasis, Epstein-Barr virus infection and a higher death rate from NPC, indicating oncogenic roles in NPC development. The expression levels of miR-18a and Dicer1 were inversely related in NPC tissues. Further studies demonstrated that miR-18a negatively regulated Dicer1 by binding to the 3 untranslated regions of Dicer1. In vitro and in vivo biological function assays showed that miR-18a promoted the growth, migration and invasion of NPC cells by regulating Dicer1 expression, which caused the global downregulation of miRNA expression levels including miR-200 family and miR-143. Furthermore, we found that the epithelial mesenchymal transition marker E-cadherin and the oncogene K-Ras were aberrantly expressed after miR-18a transduction, and these alterations were directly induced by downregulation of the miR-200 family and miR-143. Collectively, our findings indicate that miR-18a plays an oncogenic role in the development of NPC by widespread downregulation of the miRNome and could be a potential therapeutic target for NPC.
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Circulating miR-17, miR-20a, miR-29c, and miR-223 combined as non-invasive biomarkers in nasopharyngeal carcinoma.
PLoS ONE
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MicroRNAs have been considered as a kind of potential novel biomarker for cancer detection due to their remarkable stability in the blood and the characteristics of their expression profile in many diseases.
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An in silico analysis of dynamic changes in microRNA expression profiles in stepwise development of nasopharyngeal carcinoma.
BMC Med Genomics
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MicroRNAs (miRNAs) are small non-coding RNAs that participate in the spatiotemporal regulation of messenger RNA (mRNA) and protein synthesis. Recent studies have shown that some miRNAs are involved in the progression of nasopharyngeal carcinoma (NPC). However, the aberrant miRNAs implicated in different clinical stages of NPC remain unknown and their functions have not been systematically studied.
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What is Visualize?

JoVE Visualize is a tool created to match the last 5 years of PubMed publications to methods in JoVE's video library.

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In developing our video relationships, we compare around 5 million PubMed articles to our library of over 4,500 methods videos. In some cases the language used in the PubMed abstracts makes matching that content to a JoVE video difficult. In other cases, there happens not to be any content in our video library that is relevant to the topic of a given abstract. In these cases, our algorithms are trying their best to display videos with relevant content, which can sometimes result in matched videos with only a slight relation.